AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene024698

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024698.t1 MTR_5g009080 99.376 481 3 0 1 481 1 481 0.0 990
MS.gene024698.t1 MTR_4g076140 75.783 479 108 6 4 481 1 472 0.0 742
MS.gene024698.t1 MTR_3g022830 54.139 447 193 5 34 480 4 438 7.63e-170 486
MS.gene024698.t1 MTR_7g027190 34.658 453 209 7 45 478 289 673 5.81e-83 270
MS.gene024698.t1 MTR_1g106590 88.983 118 13 0 50 167 124 241 9.84e-67 218
MS.gene024698.t1 MTR_1g106590 90.722 97 9 0 286 382 244 340 4.84e-39 145
MS.gene024698.t1 MTR_5g097480 30.254 433 233 8 53 478 174 544 1.15e-59 205
MS.gene024698.t1 MTR_8g442410 30.455 440 236 10 49 479 148 526 1.23e-59 205
MS.gene024698.t1 MTR_1g029420 28.605 430 240 7 55 478 224 592 9.58e-54 190
MS.gene024698.t1 MTR_3g089055 28.140 430 242 7 55 478 202 570 2.43e-52 186
MS.gene024698.t1 MTR_3g089055 28.140 430 242 7 55 478 197 565 2.47e-52 186
MS.gene024698.t1 MTR_4g064160 28.009 432 237 7 56 477 316 683 9.18e-47 172
MS.gene024698.t1 MTR_4g064120 25.959 443 260 6 42 475 240 623 5.89e-46 169
MS.gene024698.t1 MTR_4g104020 27.563 439 249 13 50 479 143 521 8.36e-46 167
MS.gene024698.t1 MTR_4g064150 27.189 434 248 7 53 477 358 732 1.41e-45 169
MS.gene024698.t1 MTR_3g022580 42.857 203 75 5 279 481 25 186 4.02e-45 156
MS.gene024698.t1 MTR_4g064200 26.620 432 247 7 53 477 281 649 4.10e-45 167
MS.gene024698.t1 MTR_4g064180 25.734 443 261 7 42 475 240 623 3.88e-43 161
MS.gene024698.t1 MTR_7g062120 27.040 429 247 7 56 478 372 740 2.94e-42 160
MS.gene024698.t1 MTR_2g097310 26.531 441 258 9 42 477 258 637 3.25e-42 159
MS.gene024698.t1 MTR_4g133660 26.512 430 246 10 56 477 175 542 4.44e-42 157
MS.gene024698.t1 MTR_1g069725 28.899 436 236 11 48 480 101 465 1.07e-41 155
MS.gene024698.t1 MTR_2g097390 25.792 442 262 9 41 477 260 640 3.86e-41 156
MS.gene024698.t1 MTR_7g074650 41.146 192 108 2 60 249 442 630 2.40e-39 152
MS.gene024698.t1 MTR_7g074650 38.255 149 87 3 333 478 646 792 3.10e-23 103
MS.gene024698.t1 MTR_2g097350 25.850 441 261 9 42 477 260 639 3.84e-39 150
MS.gene024698.t1 MTR_4g077760 26.147 436 242 9 53 478 190 555 4.08e-38 146
MS.gene024698.t1 MTR_2g034280 27.056 462 256 12 33 478 179 575 1.47e-37 145
MS.gene024698.t1 MTR_4g102790 33.023 215 140 1 49 259 285 499 2.02e-37 145
MS.gene024698.t1 MTR_4g102790 32.692 156 98 5 328 477 507 661 2.87e-19 91.3
MS.gene024698.t1 MTR_3g065980 34.685 222 131 3 44 260 160 372 2.20e-36 141
MS.gene024698.t1 MTR_3g065980 37.576 165 90 3 327 478 376 540 6.66e-30 123
MS.gene024698.t1 MTR_2g097380 24.700 417 223 9 64 477 232 560 1.95e-34 136
MS.gene024698.t1 MTR_2g097463 27.166 427 250 10 53 477 287 654 1.99e-34 137
MS.gene024698.t1 MTR_2g089100 24.943 441 274 9 50 477 46 442 5.14e-34 133
MS.gene024698.t1 MTR_2g082090 35.238 210 129 2 53 256 209 417 1.17e-31 128
MS.gene024698.t1 MTR_2g082090 41.611 149 86 1 329 477 431 578 2.97e-29 121
MS.gene024698.t1 MTR_3g022005 54.082 98 44 1 384 481 32 128 2.56e-31 117
MS.gene024698.t1 MTR_5g094450 28.972 214 144 3 53 258 158 371 3.67e-29 120
MS.gene024698.t1 MTR_5g094450 36.879 141 88 1 338 478 393 532 6.85e-24 105
MS.gene024698.t1 MTR_3g027430 48.305 118 40 1 139 256 184 280 1.60e-28 115
MS.gene024698.t1 MTR_2g034260 24.675 462 265 12 33 478 190 584 3.43e-28 118
MS.gene024698.t1 MTR_3g056110 27.645 293 187 7 53 329 172 455 1.02e-26 113
MS.gene024698.t1 MTR_3g056110 36.170 141 89 1 338 478 403 542 3.25e-25 108
MS.gene024698.t1 MTR_2g097410 29.048 210 142 2 56 258 371 580 2.39e-26 113
MS.gene024698.t1 MTR_2g097410 28.188 149 107 0 329 477 592 740 2.55e-18 88.6
MS.gene024698.t1 MTR_2g026250 30.317 221 137 5 57 267 229 442 6.06e-26 111
MS.gene024698.t1 MTR_2g026250 34.507 142 92 1 338 479 457 597 7.59e-21 96.3
MS.gene024698.t1 MTR_7g109580 25.877 456 256 13 36 478 168 554 9.81e-26 110
MS.gene024698.t1 MTR_5g058860 26.350 463 245 15 41 477 104 496 1.19e-25 110
MS.gene024698.t1 MTR_5g015950 24.658 438 287 11 56 477 35 445 1.29e-25 109
MS.gene024698.t1 MTR_3g053270 24.658 438 287 11 56 477 35 445 1.29e-25 109
MS.gene024698.t1 MTR_4g095500 23.729 472 285 12 38 477 39 467 2.97e-25 108
MS.gene024698.t1 MTR_4g097080 29.091 220 132 4 55 258 132 343 6.67e-25 107
MS.gene024698.t1 MTR_4g097080 30.247 162 106 4 322 478 344 503 2.23e-13 72.8
MS.gene024698.t1 MTR_2g097467 31.100 209 134 2 56 258 289 493 6.74e-25 108
MS.gene024698.t1 MTR_2g097467 30.201 149 104 0 329 477 505 653 1.07e-17 86.7
MS.gene024698.t1 MTR_2g097473 31.100 209 134 2 56 258 289 493 1.80e-24 107
MS.gene024698.t1 MTR_2g097473 29.530 149 105 0 329 477 505 653 2.48e-16 82.0
MS.gene024698.t1 MTR_7g057230 27.586 348 176 10 141 477 374 656 1.09e-23 105
MS.gene024698.t1 MTR_5g015490 30.769 221 137 5 50 258 108 324 1.12e-23 103
MS.gene024698.t1 MTR_5g015490 29.032 155 103 4 329 478 338 490 5.22e-11 65.1
MS.gene024698.t1 MTR_4g076020 29.534 193 120 4 60 249 88 267 2.19e-22 99.8
MS.gene024698.t1 MTR_4g076020 39.456 147 87 2 332 478 285 429 3.70e-21 96.3
MS.gene024698.t1 MTR_2g034250 33.974 156 94 3 328 478 434 585 3.13e-22 100
MS.gene024698.t1 MTR_2g034250 29.032 248 160 6 33 264 186 433 5.91e-20 93.2
MS.gene024698.t1 MTR_8g093070 24.598 435 243 16 53 477 136 495 7.91e-22 98.6
MS.gene024698.t1 MTR_2g099110 26.512 215 148 4 47 252 65 278 1.62e-20 94.4
MS.gene024698.t1 MTR_3g021320 38.158 152 64 3 156 307 1 122 1.81e-20 87.4
MS.gene024698.t1 MTR_6g047750 23.570 437 249 12 57 478 296 662 1.01e-19 92.8
MS.gene024698.t1 MTR_1g096030 27.839 273 159 9 47 288 71 336 1.39e-19 91.7
MS.gene024698.t1 MTR_1g096030 29.143 175 106 4 323 479 286 460 7.97e-19 89.4
MS.gene024698.t1 MTR_4g026485 22.430 428 255 11 57 479 270 625 5.56e-19 90.5
MS.gene024698.t1 MTR_3g072710 28.163 245 136 8 41 255 105 339 3.29e-18 87.8
MS.gene024698.t1 MTR_3g072710 31.707 164 98 5 321 477 343 499 1.07e-13 73.6
MS.gene024698.t1 MTR_1g086970 29.570 186 117 5 297 478 303 478 7.41e-18 86.3
MS.gene024698.t1 MTR_1g086970 29.388 245 154 9 36 263 84 326 1.79e-13 72.8
MS.gene024698.t1 MTR_0092s0100 30.303 165 113 1 33 197 349 511 5.84e-13 71.6
MS.gene024698.t1 MTR_0092s0100 30.303 165 113 1 33 197 352 514 6.23e-13 71.6
MS.gene024698.t1 MTR_8g020840 23.409 440 245 16 50 466 165 535 7.35e-13 71.2
MS.gene024698.t1 MTR_3g025340 49.180 61 31 0 114 174 9 69 8.72e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024698.t1 AT1G50420 65.914 487 155 7 1 481 1 482 0.0 650
MS.gene024698.t1 AT3G54220 33.258 442 218 10 45 480 282 652 1.09e-60 210
MS.gene024698.t1 AT1G50600 29.476 458 251 10 31 478 131 526 6.53e-55 192
MS.gene024698.t1 AT1G50600 29.476 458 251 10 31 478 202 597 2.19e-54 192
MS.gene024698.t1 AT1G50600 29.476 458 251 10 31 478 206 601 2.20e-54 192
MS.gene024698.t1 AT1G21450 29.002 431 238 5 55 478 224 593 2.82e-54 192
MS.gene024698.t1 AT4G17230 26.622 447 259 7 39 478 141 525 7.37e-47 170
MS.gene024698.t1 AT4G17230 26.622 447 259 7 39 478 141 525 7.37e-47 170
MS.gene024698.t1 AT1G07520 24.099 444 268 6 45 477 143 528 8.00e-44 162
MS.gene024698.t1 AT1G07520 24.099 444 268 6 45 477 143 528 8.00e-44 162
MS.gene024698.t1 AT1G07520 24.099 444 268 6 45 477 308 693 4.38e-43 162
MS.gene024698.t1 AT1G07520 24.099 444 268 6 45 477 307 692 4.52e-43 162
MS.gene024698.t1 AT3G46600 26.097 433 253 6 55 481 3 374 3.42e-41 151
MS.gene024698.t1 AT1G07530 25.408 429 253 7 56 477 396 764 1.75e-40 155
MS.gene024698.t1 AT5G59450 25.688 436 253 9 53 478 224 598 3.01e-40 153
MS.gene024698.t1 AT3G46600 26.097 433 253 6 55 481 179 550 3.73e-40 152
MS.gene024698.t1 AT3G46600 26.097 433 253 6 55 481 211 582 6.85e-40 152
MS.gene024698.t1 AT2G29065 25.513 439 247 10 56 477 252 627 8.41e-39 149
MS.gene024698.t1 AT1G66350 35.616 219 132 4 44 260 143 354 2.80e-36 140
MS.gene024698.t1 AT1G66350 37.179 156 93 2 327 481 358 509 4.41e-31 126
MS.gene024698.t1 AT1G14920 36.161 224 129 4 44 260 160 376 1.06e-34 136
MS.gene024698.t1 AT1G14920 34.868 152 95 2 328 478 381 529 9.21e-27 113
MS.gene024698.t1 AT2G01570 35.683 227 126 5 44 260 212 428 1.84e-34 136
MS.gene024698.t1 AT2G01570 37.500 152 91 3 328 478 433 581 4.95e-27 114
MS.gene024698.t1 AT5G17490 34.404 218 132 4 47 260 151 361 2.47e-34 135
MS.gene024698.t1 AT5G17490 38.562 153 92 2 327 478 365 516 2.12e-27 115
MS.gene024698.t1 AT5G48150 28.975 283 189 4 57 329 123 403 6.90e-34 134
MS.gene024698.t1 AT5G48150 36.709 158 99 1 321 478 334 490 9.52e-27 113
MS.gene024698.t1 AT5G48150 28.975 283 189 4 57 329 123 403 6.90e-34 134
MS.gene024698.t1 AT5G48150 36.709 158 99 1 321 478 334 490 9.52e-27 113
MS.gene024698.t1 AT5G48150 28.975 283 189 4 57 329 123 403 6.90e-34 134
MS.gene024698.t1 AT5G48150 36.709 158 99 1 321 478 334 490 9.52e-27 113
MS.gene024698.t1 AT5G48150 28.975 283 189 4 57 329 123 403 6.90e-34 134
MS.gene024698.t1 AT5G48150 36.709 158 99 1 321 478 334 490 9.52e-27 113
MS.gene024698.t1 AT5G66770 27.397 438 236 13 53 478 217 584 4.04e-33 132
MS.gene024698.t1 AT3G03450 32.287 223 142 4 44 260 171 390 1.23e-32 130
MS.gene024698.t1 AT3G03450 39.355 155 92 2 325 478 392 545 2.39e-31 127
MS.gene024698.t1 AT1G63100 32.110 218 139 3 48 259 244 458 2.29e-32 130
MS.gene024698.t1 AT1G63100 36.196 163 91 3 328 477 466 628 9.55e-25 108
MS.gene024698.t1 AT1G63100 32.110 218 139 3 48 259 269 483 2.85e-32 130
MS.gene024698.t1 AT1G63100 36.196 163 91 3 328 477 491 653 1.28e-24 107
MS.gene024698.t1 AT5G41920 32.338 201 132 3 53 249 40 240 1.53e-31 125
MS.gene024698.t1 AT5G41920 36.774 155 91 3 327 480 254 402 1.93e-20 93.6
MS.gene024698.t1 AT2G37650 24.186 430 256 7 56 478 347 713 3.61e-31 127
MS.gene024698.t1 AT2G37650 24.186 430 256 7 56 478 335 701 5.03e-31 127
MS.gene024698.t1 AT2G04890 33.491 212 136 3 56 267 53 259 9.51e-31 123
MS.gene024698.t1 AT2G04890 40.000 150 89 1 329 478 265 413 9.03e-28 115
MS.gene024698.t1 AT5G52510 25.397 441 233 11 61 478 273 640 2.84e-30 124
MS.gene024698.t1 AT1G55580 36.184 152 96 1 328 478 294 445 3.76e-28 116
MS.gene024698.t1 AT1G55580 24.375 320 197 7 52 329 40 356 7.71e-15 77.0
MS.gene024698.t1 AT3G49950 24.831 443 255 12 52 477 26 407 5.26e-25 107
MS.gene024698.t1 AT2G29060 29.245 212 141 3 56 259 321 531 3.38e-24 106
MS.gene024698.t1 AT2G29060 27.273 154 103 3 329 477 542 691 2.10e-15 79.3
MS.gene024698.t1 AT2G29060 29.245 212 141 3 56 259 284 494 3.83e-24 106
MS.gene024698.t1 AT2G29060 27.273 154 103 3 329 477 505 654 1.70e-15 79.7
MS.gene024698.t1 AT4G37650 26.432 227 135 4 56 258 146 364 1.70e-22 100
MS.gene024698.t1 AT4G37650 26.415 159 107 3 329 478 372 529 6.41e-11 65.1
MS.gene024698.t1 AT4G08250 29.596 223 139 7 47 255 98 316 3.59e-20 93.6
MS.gene024698.t1 AT3G50650 30.876 217 133 4 58 267 177 383 6.17e-20 93.2
MS.gene024698.t1 AT3G50650 28.859 149 99 3 337 478 394 542 1.79e-13 73.2

Find 92 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAATTCTTGTGGAATTTA+AGG 0.089377 5.4:+3798646 None:intergenic
TATGCTTTCTGAAGAAATTA+TGG 0.230947 5.4:-3798624 MS.gene024698:CDS
TTTGAGTTTCTTGACATAAT+AGG 0.254589 5.4:+3798176 None:intergenic
TGCAGCATTGTTTGATTGTT+TGG 0.259936 5.4:-3797886 MS.gene024698:CDS
CAGCTTCTTCAGAAAGCTTA+TGG 0.264058 5.4:+3798366 None:intergenic
TCATCAGCAGAAAGAGGTTT+TGG 0.271986 5.4:-3798399 MS.gene024698:CDS
AACATACCATAATAGCTTAA+AGG 0.277960 5.4:+3797696 None:intergenic
ACAATGCTGCATAAGAATAT+AGG 0.281076 5.4:+3797898 None:intergenic
TCAAGCTATTGTTGAATCTA+TGG 0.285430 5.4:-3798543 MS.gene024698:CDS
TGCTAAGAACAGGGTTGAAT+TGG 0.312189 5.4:+3798324 None:intergenic
CTCAAATGAGGAGGACCTTC+AGG 0.312197 5.4:+3798434 None:intergenic
TTGTGGAATTTAAGGCTCTA+TGG 0.321651 5.4:+3798654 None:intergenic
ATCTATGGAAGGTGAGAAAA+TGG 0.335503 5.4:-3798528 MS.gene024698:CDS
TCTGCAGTTACATTCCCTTT+TGG 0.339325 5.4:-3798234 MS.gene024698:CDS
AACATCCCTAGAGAGATTAA+AGG 0.354098 5.4:-3797847 MS.gene024698:CDS
AAGCTATTATGGTATGTTGC+AGG 0.357915 5.4:-3797691 MS.gene024698:CDS
AATCACCTAATGATGTGTTT+TGG 0.364016 5.4:+3798120 None:intergenic
TAGTAACTTGCTGGCAAGAT+AGG 0.366004 5.4:-3797605 MS.gene024698:CDS
CAATGCTGCATAAGAATATA+GGG 0.366018 5.4:+3797899 None:intergenic
GACAAACTTCGTGTGAAAAC+CGG 0.371883 5.4:-3798281 MS.gene024698:CDS
AATGTGCCTTTAAGCTATTA+TGG 0.377946 5.4:-3797702 MS.gene024698:CDS
AGACTTGATTCGTGTGGATT+TGG 0.381293 5.4:-3797726 MS.gene024698:CDS
ATGAACCAAAACACATCATT+AGG 0.381360 5.4:-3798125 MS.gene024698:CDS
TGTGCAAATCATGTAGCTTC+TGG 0.411195 5.4:-3798812 MS.gene024698:CDS
TCACCTAGCATAGGAGGAAT+AGG 0.416008 5.4:-3798911 MS.gene024698:CDS
TATAGAATGAGAGAAGAAAA+TGG 0.416830 5.4:-3797633 MS.gene024698:CDS
CTGATAGGATACTTAAAACT+TGG 0.427400 5.4:-3798685 MS.gene024698:CDS
AGAAGGTTTCTACAGAGTTA+TGG 0.432910 5.4:-3797666 MS.gene024698:CDS
TCACTGAAGCACTTGCTGAT+AGG 0.433040 5.4:-3798700 MS.gene024698:CDS
ATGGTTGTGTAGTAACTTGC+TGG 0.448692 5.4:-3797614 MS.gene024698:CDS
TCGGTTACAACCATGACTTT+TGG 0.460163 5.4:+3797972 None:intergenic
TCACAACGGTTCAACATTGA+TGG 0.461983 5.4:-3797937 MS.gene024698:CDS
TTGCACAAGTGATTAACAAA+TGG 0.462673 5.4:+3798828 None:intergenic
CAACAACAGCTTGGAGAGCT+AGG 0.464849 5.4:-3797572 MS.gene024698:CDS
TCTCAACCTTTAATCTCTCT+AGG 0.466126 5.4:+3797841 None:intergenic
ATTCATCATCCAAGGCCAAA+AGG 0.470579 5.4:+3798219 None:intergenic
GATGATGTTGAATCGGGACT+TGG 0.470973 5.4:+3798065 None:intergenic
GTTGTGATTCGAGTCCTGTT+CGG 0.477667 5.4:+3797953 None:intergenic
GAATCTATGTTACAAGAAGA+AGG 0.480629 5.4:-3798986 MS.gene024698:CDS
AGAATTGAAATCTGAGGAAA+GGG 0.483022 5.4:-3798864 MS.gene024698:CDS
ATATATGCCACCTTCAAGAA+AGG 0.483743 5.4:+3798575 None:intergenic
ATTATGTCAAGAAACTCAAA+TGG 0.483850 5.4:-3798173 MS.gene024698:CDS
CCTCCTCATTTGAGAATCAC+AGG 0.490720 5.4:-3798425 MS.gene024698:CDS
AAGGTTGAGAAGATGCTGTT+TGG 0.498109 5.4:-3797828 MS.gene024698:CDS
AGAAAGCATAATAATTCTTG+TGG 0.500494 5.4:+3798637 None:intergenic
CTCTGCATAGTGTCTCCATC+AGG 0.502156 5.4:+3798737 None:intergenic
AGTCATGGTTGTAACCGAAC+AGG 0.503090 5.4:-3797967 MS.gene024698:CDS
CGACGATGATGATGTTGAAT+CGG 0.503259 5.4:+3798058 None:intergenic
GCTAGGTGAAACTGACATCA+TGG 0.505670 5.4:+3798925 None:intergenic
GAGAATTGAAATCTGAGGAA+AGG 0.506938 5.4:-3798865 MS.gene024698:CDS
TCTCTACTAAGCCATGGATA+AGG 0.507017 5.4:+3798884 None:intergenic
AGGATACTTAAAACTTGGCC+TGG 0.515401 5.4:-3798680 MS.gene024698:CDS
CTACAGAGTTATGGTTGTGA+GGG 0.516522 5.4:-3797657 MS.gene024698:CDS
TCACAGCTTGCAACTCCTGA+TGG 0.516747 5.4:-3798752 MS.gene024698:CDS
TTCATCATCCAAGGCCAAAA+GGG 0.519127 5.4:+3798220 None:intergenic
TTCAATTCTCTACTAAGCCA+TGG 0.520245 5.4:+3798878 None:intergenic
CTCAACCTTTAATCTCTCTA+GGG 0.522051 5.4:+3797842 None:intergenic
TATATGCCACCTTCAAGAAA+GGG 0.525767 5.4:+3798576 None:intergenic
TTGTGAAGGAGCTGAAAGAA+AGG 0.532875 5.4:-3797781 MS.gene024698:CDS
AAAAGAGCAATCCATTGTGC+AGG 0.535750 5.4:+3798476 None:intergenic
AGGGATTCATCAGCAGAAAG+AGG 0.542783 5.4:-3798405 MS.gene024698:CDS
TTCATCTGCTTCAATGAATG+TGG 0.545313 5.4:-3798027 MS.gene024698:CDS
TTTAAGGCTCTATGGAGTCC+AGG 0.546774 5.4:+3798662 None:intergenic
ACTAATTGCAAGCGCCTCGC+CGG 0.552940 5.4:+3798262 None:intergenic
GACGATGATGATGTTGAATC+GGG 0.555408 5.4:+3798059 None:intergenic
ATGGTATGTTGCAGGCAAGA+AGG 0.557823 5.4:-3797683 MS.gene024698:CDS
GATCCTATTCCTCCTATGCT+AGG 0.562234 5.4:+3798908 None:intergenic
TCAGTTTCACCTAGCATAGG+AGG 0.562988 5.4:-3798917 MS.gene024698:CDS
AGAACAGGGTTGAATTGGAA+TGG 0.565131 5.4:+3798329 None:intergenic
TCTACAGAGTTATGGTTGTG+AGG 0.567967 5.4:-3797658 MS.gene024698:CDS
ATTGAAGCAGATGAAGATGC+TGG 0.572949 5.4:+3798035 None:intergenic
GAGTTTGTCACCAAAAGTCA+TGG 0.575658 5.4:-3797982 MS.gene024698:CDS
AAGAACATAATTGCTTGTGA+AGG 0.580873 5.4:-3797795 MS.gene024698:CDS
ATCCCTGTGATTCTCAAATG+AGG 0.584689 5.4:+3798422 None:intergenic
TGAGGATGATTCATCATCCA+AGG 0.589137 5.4:+3798211 None:intergenic
AATCTATGTTACAAGAAGAA+GGG 0.598643 5.4:-3798985 MS.gene024698:CDS
GAATAGGATCACCTTATCCA+TGG 0.599511 5.4:-3798895 MS.gene024698:CDS
GCTATTGTTGAATCTATGGA+AGG 0.607832 5.4:-3798539 MS.gene024698:CDS
TGAGAAGATGCTGTTTGGTG+AGG 0.611876 5.4:-3797823 MS.gene024698:CDS
ATGTCAGTTTCACCTAGCAT+AGG 0.614550 5.4:-3798920 MS.gene024698:CDS
ATCATGGAAAATAACTGTAG+AGG 0.617197 5.4:+3798941 None:intergenic
CTCCTCATTTGAGAATCACA+GGG 0.617281 5.4:-3798424 MS.gene024698:CDS
TTTCTAGCTTGCTAAGAACA+GGG 0.622441 5.4:+3798315 None:intergenic
ACGGTTCAACATTGATGGAG+AGG 0.639461 5.4:-3797932 MS.gene024698:CDS
TAGTAGAGAATTGAAATCTG+AGG 0.644490 5.4:-3798870 MS.gene024698:CDS
TGGATGATGAATCATCCTCA+AGG 0.648643 5.4:-3798208 MS.gene024698:CDS
ATGCTGCTGAACCTGCACAA+TGG 0.650823 5.4:-3798487 MS.gene024698:CDS
GAATTGAAATCTGAGGAAAG+GGG 0.651416 5.4:-3798863 MS.gene024698:CDS
TTTATGAGACTTGATTCGTG+TGG 0.658284 5.4:-3797732 MS.gene024698:CDS
CCTGTGATTCTCAAATGAGG+AGG 0.663636 5.4:+3798425 None:intergenic
ACTTCGTGTGAAAACCGGCG+AGG 0.733150 5.4:-3798276 MS.gene024698:CDS
GAACAGGACTCGAATCACAA+CGG 0.767714 5.4:-3797951 MS.gene024698:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATTCTTGTGGAATTTA+AGG + chr5.4:3797912-3797931 None:intergenic 20.0%
!! TGATTTACTTGAAAAAGATA+TGG - chr5.4:3798453-3798472 MS.gene024698:CDS 20.0%
! AACATACCATAATAGCTTAA+AGG + chr5.4:3798862-3798881 None:intergenic 25.0%
! AATCTATGTTACAAGAAGAA+GGG - chr5.4:3797570-3797589 MS.gene024698:CDS 25.0%
! ATTATGTCAAGAAACTCAAA+TGG - chr5.4:3798382-3798401 MS.gene024698:CDS 25.0%
! CTTGAAAAAGATATGGTTAA+TGG - chr5.4:3798460-3798479 MS.gene024698:CDS 25.0%
! GACATGAAAAACTTGATAAA+TGG - chr5.4:3798800-3798819 MS.gene024698:CDS 25.0%
! TATAGAATGAGAGAAGAAAA+TGG - chr5.4:3798922-3798941 MS.gene024698:CDS 25.0%
!! AGAAAGCATAATAATTCTTG+TGG + chr5.4:3797921-3797940 None:intergenic 25.0%
!! TATGCTTTCTGAAGAAATTA+TGG - chr5.4:3797931-3797950 MS.gene024698:CDS 25.0%
!! TTTGAGTTTCTTGACATAAT+AGG + chr5.4:3798382-3798401 None:intergenic 25.0%
AAGAACATAATTGCTTGTGA+AGG - chr5.4:3798760-3798779 MS.gene024698:CDS 30.0%
AATGTGCCTTTAAGCTATTA+TGG - chr5.4:3798853-3798872 MS.gene024698:CDS 30.0%
ACAATGCTGCATAAGAATAT+AGG + chr5.4:3798660-3798679 None:intergenic 30.0%
AGAATTGAAATCTGAGGAAA+GGG - chr5.4:3797691-3797710 MS.gene024698:CDS 30.0%
ATCATGGAAAATAACTGTAG+AGG + chr5.4:3797617-3797636 None:intergenic 30.0%
ATGAACCAAAACACATCATT+AGG - chr5.4:3798430-3798449 MS.gene024698:CDS 30.0%
CAATGCTGCATAAGAATATA+GGG + chr5.4:3798659-3798678 None:intergenic 30.0%
CTGATAGGATACTTAAAACT+TGG - chr5.4:3797870-3797889 MS.gene024698:CDS 30.0%
GAATCTATGTTACAAGAAGA+AGG - chr5.4:3797569-3797588 MS.gene024698:CDS 30.0%
TAGTAGAGAATTGAAATCTG+AGG - chr5.4:3797685-3797704 MS.gene024698:CDS 30.0%
TCAAGCTATTGTTGAATCTA+TGG - chr5.4:3798012-3798031 MS.gene024698:CDS 30.0%
TTGCACAAGTGATTAACAAA+TGG + chr5.4:3797730-3797749 None:intergenic 30.0%
! AATCACCTAATGATGTGTTT+TGG + chr5.4:3798438-3798457 None:intergenic 30.0%
! AGAGTTTTCTCAATGCTTTA+TGG - chr5.4:3798551-3798570 MS.gene024698:CDS 30.0%
AACATCCCTAGAGAGATTAA+AGG - chr5.4:3798708-3798727 MS.gene024698:CDS 35.0%
AAGCTATTATGGTATGTTGC+AGG - chr5.4:3798864-3798883 MS.gene024698:CDS 35.0%
AGAAGGTTTCTACAGAGTTA+TGG - chr5.4:3798889-3798908 MS.gene024698:CDS 35.0%
ATATATGCCACCTTCAAGAA+AGG + chr5.4:3797983-3798002 None:intergenic 35.0%
ATCTATGGAAGGTGAGAAAA+TGG - chr5.4:3798027-3798046 MS.gene024698:CDS 35.0%
CTCAACCTTTAATCTCTCTA+GGG + chr5.4:3798716-3798735 None:intergenic 35.0%
GAATTGAAATCTGAGGAAAG+GGG - chr5.4:3797692-3797711 MS.gene024698:CDS 35.0%
GAGAATTGAAATCTGAGGAA+AGG - chr5.4:3797690-3797709 MS.gene024698:CDS 35.0%
GCTATTGTTGAATCTATGGA+AGG - chr5.4:3798016-3798035 MS.gene024698:CDS 35.0%
TATATGCCACCTTCAAGAAA+GGG + chr5.4:3797982-3798001 None:intergenic 35.0%
TCTCAACCTTTAATCTCTCT+AGG + chr5.4:3798717-3798736 None:intergenic 35.0%
TGCAGCATTGTTTGATTGTT+TGG - chr5.4:3798669-3798688 MS.gene024698:CDS 35.0%
TTCAATTCTCTACTAAGCCA+TGG + chr5.4:3797680-3797699 None:intergenic 35.0%
TTCATCTGCTTCAATGAATG+TGG - chr5.4:3798528-3798547 MS.gene024698:CDS 35.0%
! AATAGGAGTTTTTCTCCTTG+AGG + chr5.4:3798365-3798384 None:intergenic 35.0%
! TTGTGGAATTTAAGGCTCTA+TGG + chr5.4:3797904-3797923 None:intergenic 35.0%
! TTTATGAGACTTGATTCGTG+TGG - chr5.4:3798823-3798842 MS.gene024698:CDS 35.0%
!! TTTCTAGCTTGCTAAGAACA+GGG + chr5.4:3798243-3798262 None:intergenic 35.0%
!!! AAAGAGGTTTTGGAGCAAAT+GGG - chr5.4:3798166-3798185 MS.gene024698:CDS 35.0%
!!! CTTTGTTTTCAACAACAGCT+TGG - chr5.4:3798974-3798993 MS.gene024698:CDS 35.0%
!!! TGTGTTTTGGTTCATAAGCA+TGG + chr5.4:3798425-3798444 None:intergenic 35.0%
!!! TTTTCTAGCTTGCTAAGAAC+AGG + chr5.4:3798244-3798263 None:intergenic 35.0%
AAAAGAGCAATCCATTGTGC+AGG + chr5.4:3798082-3798101 None:intergenic 40.0%
AAGGTTGAGAAGATGCTGTT+TGG - chr5.4:3798727-3798746 MS.gene024698:CDS 40.0%
AGAACAGGGTTGAATTGGAA+TGG + chr5.4:3798229-3798248 None:intergenic 40.0%
AGGATACTTAAAACTTGGCC+TGG - chr5.4:3797875-3797894 MS.gene024698:CDS 40.0%
ATCCCTGTGATTCTCAAATG+AGG + chr5.4:3798136-3798155 None:intergenic 40.0%
ATGTCAGTTTCACCTAGCAT+AGG - chr5.4:3797635-3797654 MS.gene024698:CDS 40.0%
ATTCATCATCCAAGGCCAAA+AGG + chr5.4:3798339-3798358 None:intergenic 40.0%
ATTGAAGCAGATGAAGATGC+TGG + chr5.4:3798523-3798542 None:intergenic 40.0%
CAGCTTCTTCAGAAAGCTTA+TGG + chr5.4:3798192-3798211 None:intergenic 40.0%
CTACAGAGTTATGGTTGTGA+GGG - chr5.4:3798898-3798917 MS.gene024698:CDS 40.0%
CTCCTCATTTGAGAATCACA+GGG - chr5.4:3798131-3798150 MS.gene024698:CDS 40.0%
GAATAGGATCACCTTATCCA+TGG - chr5.4:3797660-3797679 MS.gene024698:CDS 40.0%
GACAAACTTCGTGTGAAAAC+CGG - chr5.4:3798274-3798293 MS.gene024698:CDS 40.0%
GAGTTTGTCACCAAAAGTCA+TGG - chr5.4:3798573-3798592 MS.gene024698:CDS 40.0%
TCACAACGGTTCAACATTGA+TGG - chr5.4:3798618-3798637 MS.gene024698:CDS 40.0%
TCTACAGAGTTATGGTTGTG+AGG - chr5.4:3798897-3798916 MS.gene024698:CDS 40.0%
TCTCTACTAAGCCATGGATA+AGG + chr5.4:3797674-3797693 None:intergenic 40.0%
TGAGGATGATTCATCATCCA+AGG + chr5.4:3798347-3798366 None:intergenic 40.0%
TGCTAAGAACAGGGTTGAAT+TGG + chr5.4:3798234-3798253 None:intergenic 40.0%
TGGATGATGAATCATCCTCA+AGG - chr5.4:3798347-3798366 MS.gene024698:CDS 40.0%
TGTGCAAATCATGTAGCTTC+TGG - chr5.4:3797743-3797762 MS.gene024698:CDS 40.0%
TTCATCATCCAAGGCCAAAA+GGG + chr5.4:3798338-3798357 None:intergenic 40.0%
TTGTGAAGGAGCTGAAAGAA+AGG - chr5.4:3798774-3798793 MS.gene024698:CDS 40.0%
! AGACTTGATTCGTGTGGATT+TGG - chr5.4:3798829-3798848 MS.gene024698:CDS 40.0%
! ATGGTTGTGTAGTAACTTGC+TGG - chr5.4:3798941-3798960 MS.gene024698:CDS 40.0%
! CGACGATGATGATGTTGAAT+CGG + chr5.4:3798500-3798519 None:intergenic 40.0%
! GACGATGATGATGTTGAATC+GGG + chr5.4:3798499-3798518 None:intergenic 40.0%
! TCATAAGCATGGCTTTTTGC+AGG + chr5.4:3798414-3798433 None:intergenic 40.0%
! TCATCAGCAGAAAGAGGTTT+TGG - chr5.4:3798156-3798175 MS.gene024698:CDS 40.0%
! TCGGTTACAACCATGACTTT+TGG + chr5.4:3798586-3798605 None:intergenic 40.0%
! TCTGCAGTTACATTCCCTTT+TGG - chr5.4:3798321-3798340 MS.gene024698:CDS 40.0%
! TGAGCTTTTCCCTTTCTTGA+AGG - chr5.4:3797970-3797989 MS.gene024698:CDS 40.0%
!! TAGTAACTTGCTGGCAAGAT+AGG - chr5.4:3798950-3798969 MS.gene024698:CDS 40.0%
!!! GAAAGAGGTTTTGGAGCAAA+TGG - chr5.4:3798165-3798184 MS.gene024698:CDS 40.0%
ACGGTTCAACATTGATGGAG+AGG - chr5.4:3798623-3798642 MS.gene024698:CDS 45.0%
AGGGATTCATCAGCAGAAAG+AGG - chr5.4:3798150-3798169 MS.gene024698:CDS 45.0%
AGTCATGGTTGTAACCGAAC+AGG - chr5.4:3798588-3798607 MS.gene024698:CDS 45.0%
ATGGTATGTTGCAGGCAAGA+AGG - chr5.4:3798872-3798891 MS.gene024698:CDS 45.0%
CCTCCTCATTTGAGAATCAC+AGG - chr5.4:3798130-3798149 MS.gene024698:CDS 45.0%
CCTGTGATTCTCAAATGAGG+AGG + chr5.4:3798133-3798152 None:intergenic 45.0%
GAACAGGACTCGAATCACAA+CGG - chr5.4:3798604-3798623 MS.gene024698:CDS 45.0%
GATCCTATTCCTCCTATGCT+AGG + chr5.4:3797650-3797669 None:intergenic 45.0%
GATGATGTTGAATCGGGACT+TGG + chr5.4:3798493-3798512 None:intergenic 45.0%
GCTAGGTGAAACTGACATCA+TGG + chr5.4:3797633-3797652 None:intergenic 45.0%
GTTGTGATTCGAGTCCTGTT+CGG + chr5.4:3798605-3798624 None:intergenic 45.0%
TCACCTAGCATAGGAGGAAT+AGG - chr5.4:3797644-3797663 MS.gene024698:CDS 45.0%
TCAGTTTCACCTAGCATAGG+AGG - chr5.4:3797638-3797657 MS.gene024698:CDS 45.0%
! GCTTTTCCCTTTCTTGAAGG+TGG - chr5.4:3797973-3797992 MS.gene024698:CDS 45.0%
! GTTACATTCCCTTTTGGCCT+TGG - chr5.4:3798327-3798346 MS.gene024698:CDS 45.0%
! TCACTGAAGCACTTGCTGAT+AGG - chr5.4:3797855-3797874 MS.gene024698:CDS 45.0%
! TGAGAAGATGCTGTTTGGTG+AGG - chr5.4:3798732-3798751 MS.gene024698:CDS 45.0%
! TTTAAGGCTCTATGGAGTCC+AGG + chr5.4:3797896-3797915 None:intergenic 45.0%
!! GTTTTGAGTGCAAGACCTGA+AGG - chr5.4:3798106-3798125 MS.gene024698:CDS 45.0%
ATGCTGCTGAACCTGCACAA+TGG - chr5.4:3798068-3798087 MS.gene024698:CDS 50.0%
CAACAACAGCTTGGAGAGCT+AGG - chr5.4:3798983-3799002 MS.gene024698:CDS 50.0%
CTCAAATGAGGAGGACCTTC+AGG + chr5.4:3798124-3798143 None:intergenic 50.0%
CTCTGCATAGTGTCTCCATC+AGG + chr5.4:3797821-3797840 None:intergenic 50.0%
TCACAGCTTGCAACTCCTGA+TGG - chr5.4:3797803-3797822 MS.gene024698:CDS 50.0%
ACTAATTGCAAGCGCCTCGC+CGG + chr5.4:3798296-3798315 None:intergenic 55.0%
ACTTCGTGTGAAAACCGGCG+AGG - chr5.4:3798279-3798298 MS.gene024698:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 3797566 3799011 3797566 ID=MS.gene024698
chr5.4 mRNA 3797566 3799011 3797566 ID=MS.gene024698.t1;Parent=MS.gene024698
chr5.4 exon 3797566 3799011 3797566 ID=MS.gene024698.t1.exon1;Parent=MS.gene024698.t1
chr5.4 CDS 3797566 3799011 3797566 ID=cds.MS.gene024698.t1;Parent=MS.gene024698.t1
Gene Sequence

>MS.gene024698

ATGGAATCTATGTTACAAGAAGAAGGGTCTTCATCAGTAACTTGTTCACCTCTACAGTTATTTTCCATGATGTCAGTTTCACCTAGCATAGGAGGAATAGGATCACCTTATCCATGGCTTAGTAGAGAATTGAAATCTGAGGAAAGGGGTTTGTATTTGATCCATTTGTTAATCACTTGTGCAAATCATGTAGCTTCTGGTAGTCTTGAAAGTGCAAACACAACACTTGAACAGATTTCACAGCTTGCAACTCCTGATGGAGACACTATGCAGAGAATTGCTGCTTATTTCACTGAAGCACTTGCTGATAGGATACTTAAAACTTGGCCTGGACTCCATAGAGCCTTAAATTCCACAAGAATTATTATGCTTTCTGAAGAAATTATGGTTCAGAAGTTTTTCTTTGAGCTTTTCCCTTTCTTGAAGGTGGCATATATTTTGACAAATCAAGCTATTGTTGAATCTATGGAAGGTGAGAAAATGGTTCATATTATTGATCTTAATGCTGCTGAACCTGCACAATGGATTGCTCTTTTACAAGTTTTGAGTGCAAGACCTGAAGGTCCTCCTCATTTGAGAATCACAGGGATTCATCAGCAGAAAGAGGTTTTGGAGCAAATGGGCCATAAGCTTTCTGAAGAAGCTGAGAAATTAGATATACCATTCCAATTCAACCCTGTTCTTAGCAAGCTAGAAAATCTTGATTTCGACAAACTTCGTGTGAAAACCGGCGAGGCGCTTGCAATTAGTTCTATTCTGCAGTTACATTCCCTTTTGGCCTTGGATGATGAATCATCCTCAAGGAGAAAAACTCCTATTATGTCAAGAAACTCAAATGGACTTCACCTGCAAAAAGCCATGCTTATGAACCAAAACACATCATTAGGTGATTTACTTGAAAAAGATATGGTTAATGGCTATAGTCCAAGTCCCGATTCAACATCATCATCGTCGCCAGCATCTTCATCTGCTTCAATGAATGTGGAGAGTTTTCTCAATGCTTTATGGAGTTTGTCACCAAAAGTCATGGTTGTAACCGAACAGGACTCGAATCACAACGGTTCAACATTGATGGAGAGGCTGCTTGAAGCCCTATATTCTTATGCAGCATTGTTTGATTGTTTGGAGTCAACTGTATCAAGAACATCCCTAGAGAGATTAAAGGTTGAGAAGATGCTGTTTGGTGAGGAAATCAAGAACATAATTGCTTGTGAAGGAGCTGAAAGAAAGGAAAGACATGAAAAACTTGATAAATGGTTTATGAGACTTGATTCGTGTGGATTTGGTAATGTGCCTTTAAGCTATTATGGTATGTTGCAGGCAAGAAGGTTTCTACAGAGTTATGGTTGTGAGGGATATAGAATGAGAGAAGAAAATGGTTGTGTAGTAACTTGCTGGCAAGATAGGTCTTTGTTTTCAACAACAGCTTGGAGAGCTAGGAAGTAA
Protein sequence

>MS.gene024698.t1

MESMLQEEGSSSVTCSPLQLFSMMSVSPSIGGIGSPYPWLSRELKSEERGLYLIHLLITCANHVASGSLESANTTLEQISQLATPDGDTMQRIAAYFTEALADRILKTWPGLHRALNSTRIIMLSEEIMVQKFFFELFPFLKVAYILTNQAIVESMEGEKMVHIIDLNAAEPAQWIALLQVLSARPEGPPHLRITGIHQQKEVLEQMGHKLSEEAEKLDIPFQFNPVLSKLENLDFDKLRVKTGEALAISSILQLHSLLALDDESSSRRKTPIMSRNSNGLHLQKAMLMNQNTSLGDLLEKDMVNGYSPSPDSTSSSSPASSSASMNVESFLNALWSLSPKVMVVTEQDSNHNGSTLMERLLEALYSYAALFDCLESTVSRTSLERLKVEKMLFGEEIKNIIACEGAERKERHEKLDKWFMRLDSCGFGNVPLSYYGMLQARRFLQSYGCEGYRMREENGCVVTCWQDRSLFSTTAWRARK