AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene024962

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024962.t1 MTR_5g005110 91.892 333 18 3 1 326 1 331 0.0 619
MS.gene024962.t1 MTR_5g005110 90.943 265 15 3 1 258 1 263 6.19e-173 480
MS.gene024962.t1 MTR_1g106460 49.271 343 125 12 1 309 1 328 1.50e-83 256
MS.gene024962.t1 MTR_1g106470 48.243 313 130 8 1 308 1 286 7.61e-78 239
MS.gene024962.t1 MTR_3g103030 50.909 220 92 7 93 307 111 319 2.62e-56 185
MS.gene024962.t1 MTR_1g106460 41.216 296 115 12 1 257 1 276 5.58e-46 157
MS.gene024962.t1 MTR_1g018090 58.491 159 53 4 139 294 201 349 2.67e-44 155
MS.gene024962.t1 MTR_1g106470 40.909 264 125 7 1 260 1 237 8.91e-44 150
MS.gene024962.t1 MTR_1g106480 56.081 148 30 4 193 308 12 156 1.12e-39 137
MS.gene024962.t1 MTR_5g079500 62.500 72 27 0 216 287 215 286 1.31e-25 104
MS.gene024962.t1 MTR_1g018090 49.206 126 51 4 139 261 201 316 5.26e-19 86.3
MS.gene024962.t1 MTR_4g131160 60.000 65 26 0 218 282 195 259 1.00e-18 86.7
MS.gene024962.t1 MTR_4g131160 60.000 65 26 0 218 282 198 262 1.10e-18 86.3
MS.gene024962.t1 MTR_8g093500 66.071 56 19 0 228 283 123 178 2.07e-18 84.0
MS.gene024962.t1 MTR_3g101810 61.111 54 21 0 228 281 180 233 9.36e-17 79.3
MS.gene024962.t1 MTR_7g069590 60.000 55 22 0 226 280 33 87 1.07e-16 76.6
MS.gene024962.t1 MTR_1g080890 58.462 65 27 0 218 282 192 256 1.12e-16 80.1
MS.gene024962.t1 MTR_1g019240 39.604 101 54 1 203 296 62 162 1.63e-15 75.9
MS.gene024962.t1 MTR_3g449770 45.570 79 36 2 212 290 304 375 7.61e-14 72.4
MS.gene024962.t1 MTR_1g084980 46.970 66 35 0 217 282 460 525 1.33e-13 72.0
MS.gene024962.t1 MTR_5g017040 43.939 66 37 0 230 295 116 181 7.70e-13 68.9
MS.gene024962.t1 MTR_4g033295 52.239 67 31 1 218 283 117 183 1.53e-12 65.5
MS.gene024962.t1 MTR_4g033295 54.545 66 29 1 218 282 117 182 1.75e-12 65.9
MS.gene024962.t1 MTR_1g069155 45.455 66 36 0 217 282 315 380 2.25e-12 68.2
MS.gene024962.t1 MTR_5g048860 33.951 162 87 4 129 282 280 429 3.89e-12 67.4
MS.gene024962.t1 MTR_7g053410 51.562 64 30 1 220 282 318 381 1.14e-11 65.9
MS.gene024962.t1 MTR_7g053410 51.562 64 30 1 220 282 318 381 1.26e-11 65.9
MS.gene024962.t1 MTR_7g099540 45.588 68 36 1 215 282 249 315 1.33e-11 65.5
MS.gene024962.t1 MTR_8g062240 50.000 64 31 1 220 282 152 215 1.38e-11 64.3
MS.gene024962.t1 MTR_8g012290 51.562 64 30 1 220 282 292 355 1.49e-11 65.5
MS.gene024962.t1 MTR_8g012290 51.562 64 30 1 220 282 292 355 1.51e-11 65.5
MS.gene024962.t1 MTR_3g116770 42.708 96 46 3 188 282 168 255 2.53e-11 64.3
MS.gene024962.t1 MTR_4g070320 32.520 123 65 2 178 282 85 207 2.65e-11 64.3
MS.gene024962.t1 MTR_3g116770 42.708 96 46 3 188 282 168 255 2.68e-11 64.3
MS.gene024962.t1 MTR_8g065740 42.553 94 35 2 208 282 276 369 2.89e-11 64.7
MS.gene024962.t1 MTR_1g116790 50.000 68 33 1 218 284 264 331 8.87e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024962.t1 AT2G14760 41.390 331 146 13 10 305 6 323 3.75e-61 198
MS.gene024962.t1 AT4G33880 39.828 349 147 11 10 301 6 348 1.48e-58 192
MS.gene024962.t1 AT2G14760 40.580 345 142 15 10 305 6 336 1.87e-57 189
MS.gene024962.t1 AT1G27740 67.544 114 36 1 190 302 137 250 1.66e-47 160
MS.gene024962.t1 AT5G43175 54.140 157 53 5 155 302 69 215 8.28e-45 152
MS.gene024962.t1 AT5G37800 70.000 60 18 0 228 287 210 269 2.49e-24 100
MS.gene024962.t1 AT1G66470 71.667 60 17 0 228 287 204 263 2.55e-24 100
MS.gene024962.t1 AT2G24260 63.077 65 24 0 218 282 34 98 3.64e-21 91.3
MS.gene024962.t1 AT2G24260 63.077 65 24 0 218 282 137 201 2.39e-20 90.5
MS.gene024962.t1 AT4G30980 63.077 65 24 0 218 282 129 193 2.66e-20 90.1
MS.gene024962.t1 AT4G30980 63.077 65 24 0 218 282 177 241 4.80e-20 89.7
MS.gene024962.t1 AT5G58010 58.462 65 27 0 218 282 98 162 2.05e-18 84.3
MS.gene024962.t1 AT5G01305 57.377 61 26 0 218 278 36 96 1.95e-17 79.0
MS.gene024962.t1 AT3G21330 57.143 56 24 0 226 281 276 331 4.71e-16 78.6
MS.gene024962.t1 AT4G00120 51.852 54 26 0 228 281 121 174 3.36e-15 73.6
MS.gene024962.t1 AT2G46970 32.520 123 67 4 202 308 166 288 2.02e-13 70.9
MS.gene024962.t1 AT2G46970 32.520 123 67 4 202 308 175 297 2.64e-13 70.5
MS.gene024962.t1 AT2G46970 32.520 123 67 4 202 308 197 319 2.71e-13 70.5
MS.gene024962.t1 AT2G20180 45.679 81 43 1 202 282 191 270 5.12e-13 69.7
MS.gene024962.t1 AT2G20180 45.679 81 43 1 202 282 191 270 5.12e-13 69.7
MS.gene024962.t1 AT2G20180 45.679 81 43 1 202 282 191 270 5.12e-13 69.7
MS.gene024962.t1 AT2G20180 45.679 81 43 1 202 282 262 341 5.66e-13 69.7
MS.gene024962.t1 AT2G20180 45.679 81 43 1 202 282 262 341 5.66e-13 69.7
MS.gene024962.t1 AT2G20180 45.679 81 43 1 202 282 262 341 5.66e-13 69.7
MS.gene024962.t1 AT2G20180 45.679 81 43 1 202 282 262 341 5.66e-13 69.7
MS.gene024962.t1 AT1G09530 41.463 82 44 1 214 291 328 409 6.88e-13 69.7
MS.gene024962.t1 AT1G09530 41.463 82 44 1 214 291 328 409 6.88e-13 69.7
MS.gene024962.t1 AT1G09530 41.463 82 44 1 214 291 328 409 6.88e-13 69.7
MS.gene024962.t1 AT1G09530 41.463 82 44 1 214 291 328 409 6.88e-13 69.7
MS.gene024962.t1 AT1G09530 41.463 82 44 1 214 291 328 409 6.88e-13 69.7
MS.gene024962.t1 AT1G09530 41.463 82 44 1 214 291 328 409 6.88e-13 69.7
MS.gene024962.t1 AT5G50915 46.753 77 39 2 220 295 137 212 1.76e-12 67.4
MS.gene024962.t1 AT5G50915 46.753 77 39 2 220 295 137 212 1.76e-12 67.4
MS.gene024962.t1 AT5G50915 46.753 77 39 2 220 295 137 212 1.85e-12 67.0
MS.gene024962.t1 AT5G50915 46.753 77 39 2 220 295 137 212 1.85e-12 67.0
MS.gene024962.t1 AT2G20180 45.570 79 42 1 202 280 262 339 1.96e-12 67.8
MS.gene024962.t1 AT4G36930 38.889 90 53 2 226 313 198 287 2.00e-12 67.8
MS.gene024962.t1 AT4G00050 37.607 117 56 4 174 286 69 172 3.88e-12 66.2
MS.gene024962.t1 AT1G73830 36.806 144 74 6 148 282 76 211 4.71e-12 65.5
MS.gene024962.t1 AT1G59640 43.478 92 44 3 220 310 137 221 5.15e-12 65.5
MS.gene024962.t1 AT1G73830 36.806 144 74 6 148 282 76 211 5.28e-12 65.5
MS.gene024962.t1 AT1G25330 53.125 64 29 1 220 282 105 168 5.61e-12 64.7
MS.gene024962.t1 AT4G00050 37.607 117 56 4 174 286 171 274 6.69e-12 66.2
MS.gene024962.t1 AT4G00050 37.607 117 56 4 174 286 171 274 7.02e-12 66.2
MS.gene024962.t1 AT4G09180 50.000 68 33 1 218 284 183 250 9.89e-12 64.7
MS.gene024962.t1 AT1G35460 51.515 66 31 1 218 282 180 245 1.50e-11 64.3
MS.gene024962.t1 AT1G59640 43.011 93 45 3 220 311 137 222 1.54e-11 65.1
MS.gene024962.t1 AT3G07340 51.562 64 30 1 220 282 259 322 1.71e-11 65.1
MS.gene024962.t1 AT3G07340 51.562 64 30 1 220 282 259 322 2.03e-11 65.1
MS.gene024962.t1 AT5G48560 51.562 64 30 1 220 282 302 365 2.27e-11 65.1
MS.gene024962.t1 AT5G48560 51.562 64 30 1 220 282 302 365 2.35e-11 64.7
MS.gene024962.t1 AT1G51140 50.769 65 31 1 218 281 303 367 2.37e-11 64.7
MS.gene024962.t1 AT1G26260 51.562 64 30 1 220 282 173 236 2.61e-11 64.3
MS.gene024962.t1 AT1G26260 51.562 64 30 1 220 282 202 265 2.98e-11 64.3
MS.gene024962.t1 AT1G26260 51.562 64 30 1 220 282 224 287 3.12e-11 64.3
MS.gene024962.t1 AT1G26260 51.562 64 30 1 220 282 224 287 3.12e-11 64.3
MS.gene024962.t1 AT1G26260 51.562 64 30 1 220 282 224 287 3.12e-11 64.3
MS.gene024962.t1 AT1G26260 51.562 64 30 1 220 282 224 287 3.12e-11 64.3
MS.gene024962.t1 AT4G34530 50.000 64 31 1 220 282 173 236 4.32e-11 63.5
MS.gene024962.t1 AT4G28790 39.326 89 53 1 208 296 260 347 4.78e-11 63.9
MS.gene024962.t1 AT5G67110 50.980 51 25 0 232 282 100 150 4.92e-11 62.0
MS.gene024962.t1 AT1G68920 50.000 64 31 1 220 282 303 366 9.84e-11 63.2
MS.gene024962.t1 AT1G68920 50.000 64 31 1 220 282 304 367 9.95e-11 63.2
MS.gene024962.t1 AT1G68920 50.000 64 31 1 220 282 304 367 9.95e-11 63.2
MS.gene024962.t1 AT1G68920 50.000 64 31 1 220 282 304 367 9.95e-11 63.2

Find 62 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACATTCCTCTTGTTCTT+TGG 0.181067 5.3:-498834 None:intergenic
CAGCTTTGACTATATTTCTA+TGG 0.187224 5.3:+498467 MS.gene024962:CDS
TGACATTCCTCTTGTTCTTT+GGG 0.254260 5.3:-498833 None:intergenic
TGTTGTACATGGAGTGAATT+TGG 0.258460 5.3:-498508 None:intergenic
ATTTAGCCGTAGAATCATTC+TGG 0.309924 5.3:-499004 None:intergenic
ATATAGTCAAAGCTGGTTAC+TGG 0.320414 5.3:-498459 None:intergenic
AGTTTCAGTACCCAACAAAT+TGG 0.360695 5.3:-498352 None:intergenic
AGTGAATTTGGAATGATCTA+TGG 0.365652 5.3:-498496 None:intergenic
ACCACAAGCGCAGAAAATTC+AGG 0.383330 5.3:+498621 MS.gene024962:CDS
TATCAAAATTCCAATTTGTT+GGG 0.383401 5.3:+498342 MS.gene024962:CDS
GAAAGTTACCTTAGTTCCAT+TGG 0.390559 5.3:-499447 None:intergenic
AAAGTTACCTTAGTTCCATT+GGG 0.395654 5.3:-499446 None:intergenic
CTATCAAAATTCCAATTTGT+TGG 0.398328 5.3:+498341 MS.gene024962:CDS
CTGAGCTTATTTCTCAAGAA+TGG 0.409782 5.3:+498232 MS.gene024962:CDS
GACTTTATGAACCAGTTACT+TGG 0.418424 5.3:+498291 MS.gene024962:CDS
ACGTAGCTCATGAGAAGAAT+TGG 0.419048 5.3:-498958 None:intergenic
TTCATTCCATTGTAAGCAAT+GGG 0.427456 5.3:-499756 None:intergenic
ATGAGGAGTTACCAAGTAAC+TGG 0.430149 5.3:-498302 None:intergenic
TGGGTACTGAAACTGCATTT+TGG 0.433806 5.3:+498361 MS.gene024962:CDS
GAATACAAGTCCTGGTTCTC+AGG 0.435078 5.3:+498899 MS.gene024962:CDS
GATTCTACGGCTAAATTAAG+TGG 0.448568 5.3:+499011 MS.gene024962:CDS
CCTTAGTTCCATTGGGGACA+AGG 0.451533 5.3:-499439 None:intergenic
CCTTGTCCCCAATGGAACTA+AGG 0.456805 5.3:+499439 MS.gene024962:CDS
GTTCATTCCATTGTAAGCAA+TGG 0.476694 5.3:-499757 None:intergenic
AGCTTTGACTATATTTCTAT+GGG 0.482058 5.3:+498468 MS.gene024962:CDS
TCATCAGATTGTGTTGTACA+TGG 0.498142 5.3:-498519 None:intergenic
ATGAACATTGGACTTGAACT+TGG 0.504815 5.3:+499774 MS.gene024962:CDS
ACAAGAGGAATGTCAAATGT+AGG 0.531678 5.3:+498841 MS.gene024962:CDS
CATAGAAATATAGTCAAAGC+TGG 0.533612 5.3:-498466 None:intergenic
GCTTACAATGGAATGAACAT+TGG 0.534664 5.3:+499762 MS.gene024962:CDS
AGGCTCTGTGGATCAGTAGC+AGG 0.543740 5.3:-499056 None:intergenic
TTCTCATGAGCTACGTGGAA+GGG 0.546048 5.3:+498965 MS.gene024962:CDS
TCTTGAGAAATAAGCTCAGT+AGG 0.547757 5.3:-498228 None:intergenic
GAAGAGAAAGAATAAATGAA+AGG 0.551355 5.3:+499399 MS.gene024962:CDS
TACTCTCCCATTGCTTACAA+TGG 0.559351 5.3:+499750 MS.gene024962:CDS
TTTGAGCCAGAATGATTCTA+CGG 0.561478 5.3:+498998 MS.gene024962:CDS
CTACAAAACCTTGTCCCCAA+TGG 0.563548 5.3:+499431 MS.gene024962:CDS
ACTAGTTTCTGAACCAGAAG+AGG 0.570837 5.3:+498593 MS.gene024962:CDS
TATAGGTCCCAAAGAACAAG+AGG 0.573850 5.3:+498826 MS.gene024962:intron
ACTGAAGAGAATACAAGTCC+TGG 0.575082 5.3:+498891 MS.gene024962:CDS
AACGTCTGATCCTGAGAACC+AGG 0.576280 5.3:-498909 None:intergenic
CTTCTCATGAGCTACGTGGA+AGG 0.576985 5.3:+498964 MS.gene024962:CDS
AAGATCTAGGAGCTCAAATG+AGG 0.582801 5.3:+498647 MS.gene024962:CDS
CTTGTATCAGAAGTTGAGGT+AGG 0.584296 5.3:-498420 None:intergenic
TCTCAGGATCAGACGTTAAG+CGG 0.590949 5.3:+498915 MS.gene024962:CDS
GGATATCAGCACCATGCTTG+AGG 0.592275 5.3:+499564 MS.gene024962:CDS
GCAACTTGTATCAGAAGTTG+AGG 0.594320 5.3:-498424 None:intergenic
GAAATGTTGTGATGAGAATG+AGG 0.597945 5.3:-498319 None:intergenic
AACTTGTGCAGTAACAATTG+TGG 0.601327 5.3:-498397 None:intergenic
GTTAAGCGGTTATTGTTCAG+AGG 0.601995 5.3:+498929 MS.gene024962:CDS
TTGGACAGCTTCCTCAAGCA+TGG 0.610000 5.3:-499575 None:intergenic
TACCCCAACAAAAGAAACAA+AGG 0.623892 5.3:+499800 MS.gene024962:CDS
AAAGACTTACTCTTGCATAG+AGG 0.624768 5.3:-499076 None:intergenic
AAGTTACCTTAGTTCCATTG+GGG 0.630156 5.3:-499445 None:intergenic
TTCTAAGCTCTGATGACATG+TGG 0.645185 5.3:+499724 MS.gene024962:CDS
GGAAAATCAAGATCTAGTAG+AGG 0.649455 5.3:+499032 MS.gene024962:CDS
CAGTGGACTCTACACTGCTG+AGG 0.657902 5.3:+498263 MS.gene024962:CDS
ACTCTTGCATAGAGGCTCTG+TGG 0.664485 5.3:-499068 None:intergenic
CAAGAATGGACTTCTCTCAG+TGG 0.692150 5.3:+498246 MS.gene024962:CDS
AATTCTTCTCATGAGCTACG+TGG 0.695539 5.3:+498960 MS.gene024962:CDS
GTAACAATTGTGGAATCATG+TGG 0.707912 5.3:-498387 None:intergenic
ACAATTGTGGAATCATGTGG+TGG 0.728994 5.3:-498384 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATTTAGAAGATTAATTCA+TGG - chr5.3:498688-498707 None:intergenic 20.0%
!! ACTATATATTGAACAACAAT+TGG + chr5.3:499493-499512 MS.gene024962:intron 20.0%
!! AGAAATAAAAAACAGTAGTA+AGG - chr5.3:498714-498733 None:intergenic 20.0%
!! CATTTAGAAGATTAATTCAT+GGG - chr5.3:498687-498706 None:intergenic 20.0%
!! GAAATAAAAAACAGTAGTAA+GGG - chr5.3:498713-498732 None:intergenic 20.0%
!! TCGATATACAAACATTAAAT+AGG - chr5.3:499108-499127 None:intergenic 20.0%
!!! GTTTTTACAACTTCAAATTA+AGG + chr5.3:499606-499625 MS.gene024962:CDS 20.0%
!!! TATCAAAATTCCAATTTGTT+GGG + chr5.3:498342-498361 MS.gene024962:CDS 20.0%
!!! TCTTTTCTATACAATACAAA+AGG - chr5.3:499381-499400 None:intergenic 20.0%
!!! TTTGAAATTAGAAAACTAGA+AGG - chr5.3:499137-499156 None:intergenic 20.0%
! AAAATTCAGGAAAAAGATCT+AGG + chr5.3:498634-498653 MS.gene024962:CDS 25.0%
! ACCTATAAAATCAAATGACA+CGG - chr5.3:498813-498832 None:intergenic 25.0%
! ATACAATACAAAAGGAAACA+TGG - chr5.3:499373-499392 None:intergenic 25.0%
! CTATCAAAATTCCAATTTGT+TGG + chr5.3:498341-498360 MS.gene024962:CDS 25.0%
! GAAGAGAAAGAATAAATGAA+AGG + chr5.3:499399-499418 MS.gene024962:CDS 25.0%
! GTGCTTATTCATAATGTATT+TGG + chr5.3:499299-499318 MS.gene024962:intron 25.0%
! GTTCCATTCAAATTCATATA+GGG - chr5.3:499701-499720 None:intergenic 25.0%
! GTTGTAAAAACTTCACATAT+TGG - chr5.3:499597-499616 None:intergenic 25.0%
! TGTTCAATATATAGTCTGAA+TGG - chr5.3:499488-499507 None:intergenic 25.0%
! TGTTCCATTCAAATTCATAT+AGG - chr5.3:499702-499721 None:intergenic 25.0%
!! AGCTTTGACTATATTTCTAT+GGG + chr5.3:498468-498487 MS.gene024962:CDS 25.0%
!! TGCATATCTTTTATGACTTA+CGG - chr5.3:499193-499212 None:intergenic 25.0%
!!! ATATCCTTTGTTTCTTTTGT+TGG - chr5.3:499807-499826 None:intergenic 25.0%
!!! ATGTTAATTGTTAAGTTGGT+AGG + chr5.3:499267-499286 MS.gene024962:intron 25.0%
!!! TATCCTTTGTTTCTTTTGTT+GGG - chr5.3:499806-499825 None:intergenic 25.0%
AAAACGAAATGCATATCACT+TGG - chr5.3:498742-498761 None:intergenic 30.0%
AAAATCCAAACCATAAACTG+TGG - chr5.3:498768-498787 None:intergenic 30.0%
AAAGTTACCTTAGTTCCATT+GGG - chr5.3:499449-499468 None:intergenic 30.0%
AATATATAGTCTGAATGGCA+TGG - chr5.3:499483-499502 None:intergenic 30.0%
CAACTTCAAATTAAGGTTTG+AGG + chr5.3:499613-499632 MS.gene024962:intron 30.0%
CATAGAAATATAGTCAAAGC+TGG - chr5.3:498469-498488 None:intergenic 30.0%
GATCCCTATATGAATTTGAA+TGG + chr5.3:499695-499714 MS.gene024962:intron 30.0%
GTGGATGTTAATTGTTAAGT+TGG + chr5.3:499263-499282 MS.gene024962:intron 30.0%
TTCAATCAAGCTCGATTAAA+AGG + chr5.3:499233-499252 MS.gene024962:intron 30.0%
TTCATTCCATTGTAAGCAAT+GGG - chr5.3:499759-499778 None:intergenic 30.0%
! AGTGAATTTGGAATGATCTA+TGG - chr5.3:498499-498518 None:intergenic 30.0%
! CAGCTTTGACTATATTTCTA+TGG + chr5.3:498467-498486 MS.gene024962:CDS 30.0%
! TTTTAAATGCAGAGAGAGAA+AGG - chr5.3:499644-499663 None:intergenic 30.0%
!! ATCCTTTGTTTCTTTTGTTG+GGG - chr5.3:499805-499824 None:intergenic 30.0%
!! TCGATTAAAAGGTGTTTATG+TGG + chr5.3:499244-499263 MS.gene024962:intron 30.0%
!!! TCCGTGTCATTTGATTTTAT+AGG + chr5.3:498809-498828 MS.gene024962:intron 30.0%
AAACATGGTAAGACCTTACA+TGG - chr5.3:499358-499377 None:intergenic 35.0%
AAAGACTTACTCTTGCATAG+AGG - chr5.3:499079-499098 None:intergenic 35.0%
AACTTGTGCAGTAACAATTG+TGG - chr5.3:498400-498419 None:intergenic 35.0%
AAGTTACCTTAGTTCCATTG+GGG - chr5.3:499448-499467 None:intergenic 35.0%
ACAAGAGGAATGTCAAATGT+AGG + chr5.3:498841-498860 MS.gene024962:CDS 35.0%
AGTTTCAGTACCCAACAAAT+TGG - chr5.3:498355-498374 None:intergenic 35.0%
ATATAGTCAAAGCTGGTTAC+TGG - chr5.3:498462-498481 None:intergenic 35.0%
ATGAACATTGGACTTGAACT+TGG + chr5.3:499774-499793 MS.gene024962:CDS 35.0%
ATTTAGCCGTAGAATCATTC+TGG - chr5.3:499007-499026 None:intergenic 35.0%
CAAATGAGGTAACAAACAGA+AGG + chr5.3:498661-498680 MS.gene024962:intron 35.0%
GAAAGTTACCTTAGTTCCAT+TGG - chr5.3:499450-499469 None:intergenic 35.0%
GAAATGTTGTGATGAGAATG+AGG - chr5.3:498322-498341 None:intergenic 35.0%
GACTTTATGAACCAGTTACT+TGG + chr5.3:498291-498310 MS.gene024962:CDS 35.0%
GATTCTACGGCTAAATTAAG+TGG + chr5.3:499011-499030 MS.gene024962:CDS 35.0%
GCTTACAATGGAATGAACAT+TGG + chr5.3:499762-499781 MS.gene024962:CDS 35.0%
GGAAAATCAAGATCTAGTAG+AGG + chr5.3:499032-499051 MS.gene024962:CDS 35.0%
GTAACAATTGTGGAATCATG+TGG - chr5.3:498390-498409 None:intergenic 35.0%
GTTCATTCCATTGTAAGCAA+TGG - chr5.3:499760-499779 None:intergenic 35.0%
TAATCGAGCTTGATTGAAAG+CGG - chr5.3:499231-499250 None:intergenic 35.0%
TACCCCAACAAAAGAAACAA+AGG + chr5.3:499800-499819 MS.gene024962:CDS 35.0%
TCATCAGATTGTGTTGTACA+TGG - chr5.3:498522-498541 None:intergenic 35.0%
TCTAGCTAACAAAAACTTGC+AGG + chr5.3:498548-498567 MS.gene024962:CDS 35.0%
TCTTGAGAAATAAGCTCAGT+AGG - chr5.3:498231-498250 None:intergenic 35.0%
TGACATTCCTCTTGTTCTTT+GGG - chr5.3:498836-498855 None:intergenic 35.0%
TGTTGTACATGGAGTGAATT+TGG - chr5.3:498511-498530 None:intergenic 35.0%
TTGACATTCCTCTTGTTCTT+TGG - chr5.3:498837-498856 None:intergenic 35.0%
TTTGAGCCAGAATGATTCTA+CGG + chr5.3:498998-499017 MS.gene024962:CDS 35.0%
! ACTGCTGCATTCTCTTTTAT+TGG - chr5.3:499843-499862 None:intergenic 35.0%
! ATGCAAGAGTAAGTCTTTTG+TGG + chr5.3:499081-499100 MS.gene024962:intron 35.0%
! CTGAGCTTATTTCTCAAGAA+TGG + chr5.3:498232-498251 MS.gene024962:CDS 35.0%
! GTACTTCACGTTCTGATTTT+CGG - chr5.3:498575-498594 None:intergenic 35.0%
! TACTTCACGTTCTGATTTTC+GGG - chr5.3:498574-498593 None:intergenic 35.0%
!! TTTTTGTTGATTAACCCTGC+AGG + chr5.3:499540-499559 MS.gene024962:intron 35.0%
AAGATCTAGGAGCTCAAATG+AGG + chr5.3:498647-498666 MS.gene024962:CDS 40.0%
AATCGAGCTTGATTGAAAGC+GGG - chr5.3:499230-499249 None:intergenic 40.0%
AATTCTTCTCATGAGCTACG+TGG + chr5.3:498960-498979 MS.gene024962:CDS 40.0%
ACAATTGTGGAATCATGTGG+TGG - chr5.3:498387-498406 None:intergenic 40.0%
ACGTAGCTCATGAGAAGAAT+TGG - chr5.3:498961-498980 None:intergenic 40.0%
ACTGAAGAGAATACAAGTCC+TGG + chr5.3:498891-498910 MS.gene024962:CDS 40.0%
ATCCAAACCATAAACTGTGG+TGG - chr5.3:498765-498784 None:intergenic 40.0%
ATGAGGAGTTACCAAGTAAC+TGG - chr5.3:498305-498324 None:intergenic 40.0%
ATTCTGTTGCTGTCCATGTA+AGG + chr5.3:499342-499361 MS.gene024962:intron 40.0%
CTTGTATCAGAAGTTGAGGT+AGG - chr5.3:498423-498442 None:intergenic 40.0%
GCAACTTGTATCAGAAGTTG+AGG - chr5.3:498427-498446 None:intergenic 40.0%
GTTAAGCGGTTATTGTTCAG+AGG + chr5.3:498929-498948 MS.gene024962:CDS 40.0%
TACTCTCCCATTGCTTACAA+TGG + chr5.3:499750-499769 MS.gene024962:CDS 40.0%
TATAGGTCCCAAAGAACAAG+AGG + chr5.3:498826-498845 MS.gene024962:intron 40.0%
TTCCACCACAGTTTATGGTT+TGG + chr5.3:498760-498779 MS.gene024962:intron 40.0%
! ACTAGTTTCTGAACCAGAAG+AGG + chr5.3:498593-498612 MS.gene024962:CDS 40.0%
! TCGTTTTCCACCACAGTTTA+TGG + chr5.3:498755-498774 MS.gene024962:intron 40.0%
! TTCTAAGCTCTGATGACATG+TGG + chr5.3:499724-499743 MS.gene024962:CDS 40.0%
!!! CTTGTGGTTTTATCCTCTTC+TGG - chr5.3:498609-498628 None:intergenic 40.0%
!!! TGGGTACTGAAACTGCATTT+TGG + chr5.3:498361-498380 MS.gene024962:CDS 40.0%
ACCACAAGCGCAGAAAATTC+AGG + chr5.3:498621-498640 MS.gene024962:CDS 45.0%
CTACAAAACCTTGTCCCCAA+TGG + chr5.3:499431-499450 MS.gene024962:CDS 45.0%
TCTCAGGATCAGACGTTAAG+CGG + chr5.3:498915-498934 MS.gene024962:CDS 45.0%
TTCTCATGAGCTACGTGGAA+GGG + chr5.3:498965-498984 MS.gene024962:CDS 45.0%
! CAAGAATGGACTTCTCTCAG+TGG + chr5.3:498246-498265 MS.gene024962:CDS 45.0%
! GAATACAAGTCCTGGTTCTC+AGG + chr5.3:498899-498918 MS.gene024962:CDS 45.0%
! TCCTGAATTTTCTGCGCTTG+TGG - chr5.3:498625-498644 None:intergenic 45.0%
! TTGTTGATTAACCCTGCAGG+TGG + chr5.3:499543-499562 MS.gene024962:intron 45.0%
!! CATTGGGGACAAGGTTTTGT+AGG - chr5.3:499433-499452 None:intergenic 45.0%
AACGTCTGATCCTGAGAACC+AGG - chr5.3:498912-498931 None:intergenic 50.0%
ACTCTTGCATAGAGGCTCTG+TGG - chr5.3:499071-499090 None:intergenic 50.0%
CCTTAGTTCCATTGGGGACA+AGG - chr5.3:499442-499461 None:intergenic 50.0%
CCTTGTCCCCAATGGAACTA+AGG + chr5.3:499439-499458 MS.gene024962:CDS 50.0%
CTTCTCATGAGCTACGTGGA+AGG + chr5.3:498964-498983 MS.gene024962:CDS 50.0%
GGATATCAGCACCATGCTTG+AGG + chr5.3:499564-499583 MS.gene024962:CDS 50.0%
TTGGACAGCTTCCTCAAGCA+TGG - chr5.3:499578-499597 None:intergenic 50.0%
AGGCTCTGTGGATCAGTAGC+AGG - chr5.3:499059-499078 None:intergenic 55.0%
! CAGTGGACTCTACACTGCTG+AGG + chr5.3:498263-498282 MS.gene024962:CDS 55.0%
!! GGTGCTGATATCCACCTGCA+GGG - chr5.3:499557-499576 None:intergenic 55.0%
!! TGGTGCTGATATCCACCTGC+AGG - chr5.3:499558-499577 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 498222 499866 498222 ID=MS.gene024962
chr5.3 mRNA 498222 499866 498222 ID=MS.gene024962.t1;Parent=MS.gene024962
chr5.3 exon 498222 498668 498222 ID=MS.gene024962.t1.exon1;Parent=MS.gene024962.t1
chr5.3 CDS 498222 498668 498222 ID=cds.MS.gene024962.t1;Parent=MS.gene024962.t1
chr5.3 exon 498831 499088 498831 ID=MS.gene024962.t1.exon2;Parent=MS.gene024962.t1
chr5.3 CDS 498831 499088 498831 ID=cds.MS.gene024962.t1;Parent=MS.gene024962.t1
chr5.3 exon 499395 499460 499395 ID=MS.gene024962.t1.exon3;Parent=MS.gene024962.t1
chr5.3 CDS 499395 499460 499395 ID=cds.MS.gene024962.t1;Parent=MS.gene024962.t1
chr5.3 exon 499562 499627 499562 ID=MS.gene024962.t1.exon4;Parent=MS.gene024962.t1
chr5.3 CDS 499562 499627 499562 ID=cds.MS.gene024962.t1;Parent=MS.gene024962.t1
chr5.3 exon 499723 499866 499723 ID=MS.gene024962.t1.exon5;Parent=MS.gene024962.t1
chr5.3 CDS 499723 499866 499723 ID=cds.MS.gene024962.t1;Parent=MS.gene024962.t1
Gene Sequence

>MS.gene024962

ATGGAACCTACTGAGCTTATTTCTCAAGAATGGACTTCTCTCAGTGGACTCTACACTGCTGAGGAATCTGACTTTATGAACCAGTTACTTGGTAACTCCTCATTCTCATCACAACATTTCTATCAAAATTCCAATTTGTTGGGTACTGAAACTGCATTTTGGCCACCACATGATTCCACAATTGTTACTGCACAAGTTCCTACCTCAACTTCTGATACAAGTTGCTTCAACAACAATCCAGTAACCAGCTTTGACTATATTTCTATGGGTCTTTCCATAGATCATTCCAAATTCACTCCATGTACAACACAATCTGATGAACAAGTTCTAGCTAACAAAAACTTGCAGGCCCGAAAATCAGAACGTGAAGTACTAGTTTCTGAACCAGAAGAGGATAAAACCACAAGCGCAGAAAATTCAGGAAAAAGATCTAGGAGCTCAAATGAGGTAACAAACAGAAGGCCCATGAATTAATCTTCTAAATGTTTCCCTTACTACTGTTTTTTATTTCTTCTTTCCAAGTGATATGCATTTCGTTTTCCACCACAGTTTATGGTTTGGATTTTGATTTTGATTTTCTGAAATTCTCCGTGTCATTTGATTTTATAGGTCCCAAAGAACAAGAGGAATGTCAAATGTAGGAAGATTATAAGATGTGCTTCTATAAGCACTGAAGAGAATACAAGTCCTGGTTCTCAGGATCAGACGTTAAGCGGTTATTGTTCAGAGGATGAGTCCAATTCTTCTCATGAGCTACGTGGAAGGGAATCTTCAAGTTTGAGCCAGAATGATTCTACGGCTAAATTAAGTGGAAAATCAAGATCTAGTAGAGGTCCTGCTACTGATCCACAGAGCCTCTATGCAAGAGTAAGTCTTTTGTGGTCCTATTTAATGTTTGTATATCGAACTAATCCTTCTAGTTTTCTAATTTCAAATTGATCATTTATGTTAGTTAAAATGAACTTATTCCGTAAGTCATAAAAGATATGCACTATTAGTCTATTACCCGCTTTCAATCAAGCTCGATTAAAAGGTGTTTATGTGGATGTTAATTGTTAAGTTGGTAGGTTCTAAAAAGTGCTTATTCATAATGTATTTGGTCTTTCTAATAATATATGATATTCTGTTGCTGTCCATGTAAGGTCTTACCATGTTTCCTTTTGTATTGTATAGAAAAGAAGAGAAAGAATAAATGAAAGGTTGAAAATCCTACAAAACCTTGTCCCCAATGGAACTAAGGTAACTTTCTTTCTCAATGCCATGCCATTCAGACTATATATTGAACAACAATTGGTTCATCTGCTAAAAATTCTTTTATTTTTTGTTGATTAACCCTGCAGGTGGATATCAGCACCATGCTTGAGGAAGCTGTCCAATATGTGAAGTTTTTACAACTTCAAATTAAGGTTTGAGGATTCTCCTTTCTCTCTCTGCATTTAAAATTTGAGCTCAATGCAAAGACAATTACGTACTGATCCCTATATGAATTTGAATGGAACAGCTTCTAAGCTCTGATGACATGTGGATGTACTCTCCCATTGCTTACAATGGAATGAACATTGGACTTGAACTTGGTATTACCCCAACAAAAGAAACAAAGGATATGCATAACAACATACCAATAAAAGAGAATGCAGCAGTTTAA
Protein sequence

>MS.gene024962.t1

MEPTELISQEWTSLSGLYTAEESDFMNQLLGNSSFSSQHFYQNSNLLGTETAFWPPHDSTIVTAQVPTSTSDTSCFNNNPVTSFDYISMGLSIDHSKFTPCTTQSDEQVLANKNLQARKSEREVLVSEPEEDKTTSAENSGKRSRSSNEVPKNKRNVKCRKIIRCASISTEENTSPGSQDQTLSGYCSEDESNSSHELRGRESSSLSQNDSTAKLSGKSRSSRGPATDPQSLYARKRRERINERLKILQNLVPNGTKVDISTMLEEAVQYVKFLQLQIKLLSSDDMWMYSPIAYNGMNIGLELGITPTKETKDMHNNIPIKENAAV