AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene02497

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02497.t1 MTR_3g089055 94.690 565 30 0 1 565 6 570 0.0 1080
MS.gene02497.t1 MTR_3g089055 94.690 565 30 0 1 565 1 565 0.0 1077
MS.gene02497.t1 MTR_1g029420 69.950 599 139 7 1 565 1 592 0.0 794
MS.gene02497.t1 MTR_5g097480 50.962 416 198 1 156 565 129 544 8.08e-153 449
MS.gene02497.t1 MTR_2g082090 54.324 370 169 0 196 565 210 579 3.88e-145 431
MS.gene02497.t1 MTR_3g056110 49.606 381 191 1 186 565 162 542 2.12e-139 415
MS.gene02497.t1 MTR_5g094450 51.036 386 184 3 185 565 147 532 1.64e-137 410
MS.gene02497.t1 MTR_4g133660 47.568 370 193 1 196 564 173 542 3.15e-126 381
MS.gene02497.t1 MTR_2g026250 47.170 371 195 1 196 565 226 596 1.76e-122 373
MS.gene02497.t1 MTR_7g057230 38.767 454 255 10 119 564 218 656 9.70e-87 282
MS.gene02497.t1 MTR_6g047750 40.409 391 191 10 196 565 293 662 2.33e-80 265
MS.gene02497.t1 MTR_7g027190 37.726 387 211 5 197 565 299 673 1.38e-76 256
MS.gene02497.t1 MTR_3g065980 34.987 383 218 8 199 565 173 540 7.46e-67 226
MS.gene02497.t1 MTR_8g442410 34.908 381 223 7 199 565 156 525 8.58e-65 221
MS.gene02497.t1 MTR_4g064160 31.892 370 251 1 196 564 314 683 5.74e-63 219
MS.gene02497.t1 MTR_7g074650 36.339 366 218 6 201 565 441 792 4.96e-62 219
MS.gene02497.t1 MTR_4g104020 32.723 382 235 5 197 565 148 520 5.88e-61 210
MS.gene02497.t1 MTR_7g062120 30.479 397 269 2 176 565 344 740 2.47e-59 210
MS.gene02497.t1 MTR_4g064200 31.707 369 247 4 198 564 284 649 9.97e-58 204
MS.gene02497.t1 MTR_2g097310 30.376 372 252 3 196 564 270 637 4.13e-56 199
MS.gene02497.t1 MTR_2g097350 31.818 374 244 6 196 564 272 639 4.35e-56 199
MS.gene02497.t1 MTR_4g076140 28.791 455 253 8 171 565 26 469 4.85e-56 196
MS.gene02497.t1 MTR_2g097390 30.108 372 253 3 196 564 273 640 1.41e-55 198
MS.gene02497.t1 MTR_4g097080 30.423 378 250 6 196 565 131 503 1.87e-55 195
MS.gene02497.t1 MTR_4g064150 29.144 374 260 3 196 564 359 732 5.10e-55 198
MS.gene02497.t1 MTR_4g064120 28.054 442 303 5 130 562 188 623 1.52e-54 195
MS.gene02497.t1 MTR_4g102790 35.602 382 219 14 198 564 292 661 2.09e-54 196
MS.gene02497.t1 MTR_2g097410 30.585 376 250 7 196 564 369 740 4.43e-54 196
MS.gene02497.t1 MTR_4g064180 28.217 443 303 5 130 563 188 624 4.94e-54 194
MS.gene02497.t1 MTR_5g009080 28.605 430 240 7 197 565 55 478 5.26e-54 191
MS.gene02497.t1 MTR_3g022830 28.504 421 245 5 195 565 22 436 1.26e-51 183
MS.gene02497.t1 MTR_4g077760 32.181 376 232 8 199 565 194 555 1.27e-50 183
MS.gene02497.t1 MTR_2g097467 28.385 384 269 4 182 564 275 653 2.15e-50 184
MS.gene02497.t1 MTR_5g015490 31.486 397 247 10 185 565 103 490 4.91e-50 180
MS.gene02497.t1 MTR_4g076020 33.154 371 214 10 201 565 87 429 3.37e-49 176
MS.gene02497.t1 MTR_2g097463 28.804 368 258 3 198 564 290 654 4.75e-49 180
MS.gene02497.t1 MTR_2g097473 27.097 465 311 9 117 564 200 653 1.74e-48 179
MS.gene02497.t1 MTR_3g072710 31.863 408 237 12 185 564 105 499 8.64e-48 174
MS.gene02497.t1 MTR_1g096030 29.471 397 236 12 197 565 79 459 1.20e-45 167
MS.gene02497.t1 MTR_1g069725 32.181 376 224 11 198 564 109 462 1.02e-44 165
MS.gene02497.t1 MTR_5g015950 27.962 422 242 8 196 564 33 445 3.41e-43 160
MS.gene02497.t1 MTR_3g053270 27.962 422 242 8 196 564 33 445 3.41e-43 160
MS.gene02497.t1 MTR_2g097380 27.701 361 229 4 205 564 231 560 1.26e-41 158
MS.gene02497.t1 MTR_2g099110 29.049 389 246 8 197 565 73 451 4.02e-41 154
MS.gene02497.t1 MTR_2g089100 26.018 442 279 8 156 564 16 442 2.62e-40 152
MS.gene02497.t1 MTR_4g095500 26.835 436 273 7 174 564 33 467 1.04e-38 148
MS.gene02497.t1 MTR_5g058860 30.548 383 247 10 197 564 118 496 1.69e-38 148
MS.gene02497.t1 MTR_2g034250 26.098 410 263 11 178 565 194 585 2.63e-30 125
MS.gene02497.t1 MTR_0092s0100 27.957 372 247 8 199 565 376 731 2.00e-28 120
MS.gene02497.t1 MTR_0092s0100 27.957 372 247 8 199 565 373 728 2.13e-28 120
MS.gene02497.t1 MTR_2g034260 26.555 418 250 14 178 565 194 584 4.73e-28 119
MS.gene02497.t1 MTR_7g109580 26.649 379 256 10 197 565 188 554 8.82e-27 114
MS.gene02497.t1 MTR_2g034280 25.455 385 256 10 199 565 204 575 1.15e-26 114
MS.gene02497.t1 MTR_8g093070 27.441 379 241 12 198 564 139 495 6.55e-25 108
MS.gene02497.t1 MTR_4g026485 25.995 377 247 11 199 565 270 624 1.15e-23 105
MS.gene02497.t1 MTR_1g086970 27.875 287 185 9 287 565 206 478 6.31e-20 93.2
MS.gene02497.t1 MTR_8g020840 23.632 402 265 15 190 565 163 548 6.77e-20 93.6
MS.gene02497.t1 MTR_3g022580 30.864 162 106 2 404 565 28 183 5.39e-18 82.4
MS.gene02497.t1 MTR_3g022005 37.634 93 58 0 473 565 33 125 1.88e-13 67.8
MS.gene02497.t1 MTR_8g077940 21.891 402 236 14 199 565 183 541 8.72e-13 71.2
MS.gene02497.t1 MTR_4g074310 29.237 236 121 7 201 431 2 196 1.07e-12 68.2
MS.gene02497.t1 MTR_4g074320 32.558 172 89 6 171 337 39 188 5.73e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02497.t1 AT1G21450 59.496 595 194 16 1 565 16 593 0.0 644
MS.gene02497.t1 AT5G48150 52.911 395 183 2 172 565 98 490 2.66e-146 431
MS.gene02497.t1 AT5G48150 52.911 395 183 2 172 565 98 490 2.66e-146 431
MS.gene02497.t1 AT5G48150 52.911 395 183 2 172 565 98 490 2.66e-146 431
MS.gene02497.t1 AT5G48150 52.911 395 183 2 172 565 98 490 2.66e-146 431
MS.gene02497.t1 AT1G50600 51.948 385 184 1 182 565 142 526 6.95e-142 421
MS.gene02497.t1 AT1G50600 51.948 385 184 1 182 565 213 597 5.78e-141 421
MS.gene02497.t1 AT1G50600 51.948 385 184 1 182 565 217 601 9.64e-141 421
MS.gene02497.t1 AT2G04890 51.892 370 171 1 196 565 51 413 4.50e-135 399
MS.gene02497.t1 AT4G17230 47.027 370 196 0 196 565 156 525 3.07e-124 375
MS.gene02497.t1 AT4G17230 47.027 370 196 0 196 565 156 525 3.07e-124 375
MS.gene02497.t1 AT5G52510 39.481 385 208 5 196 565 266 640 3.99e-85 277
MS.gene02497.t1 AT1G66350 37.097 372 208 9 199 565 156 506 2.79e-69 232
MS.gene02497.t1 AT3G03450 36.340 377 215 9 199 565 184 545 8.78e-68 229
MS.gene02497.t1 AT5G17490 35.242 454 246 13 136 565 87 516 2.44e-67 227
MS.gene02497.t1 AT3G54220 36.856 369 213 8 201 564 296 649 4.36e-66 227
MS.gene02497.t1 AT1G07530 33.760 391 254 3 176 564 377 764 2.17e-64 224
MS.gene02497.t1 AT5G66770 36.340 377 217 9 199 565 221 584 1.95e-60 210
MS.gene02497.t1 AT1G14920 33.780 373 225 10 199 565 173 529 1.65e-59 207
MS.gene02497.t1 AT2G01570 34.301 379 215 12 199 565 225 581 2.92e-58 204
MS.gene02497.t1 AT2G29060 31.980 394 252 5 179 564 269 654 2.05e-57 204
MS.gene02497.t1 AT2G29060 31.980 394 252 5 179 564 306 691 2.94e-57 204
MS.gene02497.t1 AT2G37650 29.919 371 257 2 196 565 333 701 1.74e-56 202
MS.gene02497.t1 AT2G37650 29.919 371 257 2 196 565 345 713 1.90e-56 202
MS.gene02497.t1 AT5G59450 29.777 403 271 5 172 565 199 598 5.31e-56 199
MS.gene02497.t1 AT2G29065 30.423 378 254 5 196 564 250 627 9.66e-54 193
MS.gene02497.t1 AT3G46600 29.919 371 258 2 196 565 2 371 1.14e-53 187
MS.gene02497.t1 AT3G46600 29.919 371 258 2 196 565 210 579 1.91e-52 189
MS.gene02497.t1 AT3G46600 29.919 371 258 2 196 565 178 547 2.03e-52 188
MS.gene02497.t1 AT5G41920 32.718 379 223 10 198 565 43 400 1.92e-51 182
MS.gene02497.t1 AT3G50650 33.983 359 209 7 221 565 198 542 1.77e-50 182
MS.gene02497.t1 AT1G07520 27.820 399 272 5 182 564 130 528 6.97e-50 181
MS.gene02497.t1 AT1G07520 27.820 399 272 5 182 564 130 528 6.97e-50 181
MS.gene02497.t1 AT1G07520 27.820 399 272 5 182 564 294 692 1.30e-49 182
MS.gene02497.t1 AT1G07520 27.820 399 272 5 182 564 295 693 1.30e-49 182
MS.gene02497.t1 AT4G37650 29.206 428 259 11 165 565 119 529 1.57e-48 177
MS.gene02497.t1 AT1G63100 31.362 389 233 8 198 564 252 628 1.43e-43 164
MS.gene02497.t1 AT1G63100 33.141 347 212 7 198 536 277 611 1.62e-43 165
MS.gene02497.t1 AT4G08250 29.825 342 220 7 233 564 145 476 4.90e-42 158
MS.gene02497.t1 AT1G55580 27.119 413 249 9 196 565 42 445 1.96e-40 152
MS.gene02497.t1 AT3G49950 27.720 386 256 9 196 564 28 407 8.45e-39 147
MS.gene02497.t1 AT1G50420 32.857 210 132 2 197 403 56 259 1.16e-30 125
MS.gene02497.t1 AT1G50420 37.748 151 91 2 417 565 330 479 9.45e-28 117
MS.gene02497.t1 AT3G13840 26.000 400 267 11 178 565 128 510 4.58e-22 100
MS.gene02497.t1 AT3G60630 25.501 349 229 10 231 565 291 622 3.72e-21 97.8
MS.gene02497.t1 AT2G45160 26.214 309 202 8 268 565 346 639 5.70e-21 97.4
MS.gene02497.t1 AT4G00150 24.069 349 234 10 222 565 232 554 9.94e-19 90.1
MS.gene02497.t1 AT5G67411 33.668 199 119 6 315 502 2 198 5.98e-18 82.8
MS.gene02497.t1 AT4G36710 23.864 352 244 9 222 565 150 485 3.36e-16 82.0

Find 116 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTATGAGAATAAGAAATT+TGG 0.178504 3.2:-76111346 None:intergenic
GGTACAACCATGTCATTAAT+TGG 0.248675 3.2:+76111239 None:intergenic
CCATGTAGGCTGCAATTCTT+TGG 0.255636 3.2:-76111943 None:intergenic
GAAAAGGGTGCACTTCATTT+TGG 0.257256 3.2:+76112880 MS.gene02497:CDS
CACCAAATCTGATCCGAAAT+TGG 0.280682 3.2:-76111516 None:intergenic
GGTATGAAGTCGCGGGAAAA+TGG 0.287860 3.2:+76112746 MS.gene02497:CDS
GCTACTGATGCTGCTAATAT+TGG 0.307471 3.2:-76111774 None:intergenic
TCCACGACCGTTACAAGTTT+TGG 0.308347 3.2:-76112529 None:intergenic
AAGAAATAACGAAAATTTCT+AGG 0.316566 3.2:+76111804 MS.gene02497:CDS
GTCCAATTTCGGATCAGATT+TGG 0.328151 3.2:+76111514 MS.gene02497:CDS
TCGAAGCAAGTGTTTGAATC+AGG 0.332729 3.2:-76112201 None:intergenic
TTGATCCTAACATTATTGAT+TGG 0.340862 3.2:+76111675 MS.gene02497:CDS
CTTATGAGAATAAGAAATTT+GGG 0.359390 3.2:-76111345 None:intergenic
TCAGCACCACGTTGCCTTGT+TGG 0.374411 3.2:-76111488 None:intergenic
GGTGCTGATCAGTCCAATTT+CGG 0.378562 3.2:+76111503 MS.gene02497:CDS
TCACTCACATTTGTGCTCAT+TGG 0.383131 3.2:-76112805 None:intergenic
GTACAAGATAAAGGAGGAAA+AGG 0.383813 3.2:+76112864 MS.gene02497:CDS
GCCGCTCGCCTCGCTGAATC+CGG 0.387906 3.2:+76111977 MS.gene02497:CDS
ATTGAGGAAGAAGAGGAATA+TGG 0.391911 3.2:+76111632 MS.gene02497:CDS
TGATCCTAACATTATTGATT+GGG 0.392056 3.2:+76111676 MS.gene02497:CDS
CGACGAATTGTCTCAATTCA+AGG 0.402603 3.2:+76111911 MS.gene02497:CDS
GGCAAGGGATATTGTTAATA+CGG 0.403210 3.2:+76112693 MS.gene02497:CDS
CATTCATAAAGAAGTTGCTT+GGG 0.414263 3.2:-76111830 None:intergenic
AACAGCAGCATATTCTGAAT+TGG 0.421315 3.2:-76111710 None:intergenic
CTGTGATAGGTACAAGATAA+AGG 0.425096 3.2:+76112855 MS.gene02497:CDS
GGGTTAACATCGATTTCCTT+TGG 0.428305 3.2:-76111311 None:intergenic
GTGAATTCAATTGAAGTTGA+TGG 0.432801 3.2:+76111554 MS.gene02497:CDS
TCAGCCCAATCAATAATGTT+AGG 0.434921 3.2:-76111680 None:intergenic
ACCGTTGAAACGCTCTCGTC+AGG 0.440496 3.2:-76112463 None:intergenic
CCATCACATGTTAGATTGAC+CGG 0.440788 3.2:+76112235 MS.gene02497:CDS
ATGCTGCTAATATTGGAATC+TGG 0.446519 3.2:-76111767 None:intergenic
CATGTAGGCTGCAATTCTTT+GGG 0.448246 3.2:-76111942 None:intergenic
ATGCAAGGAACCTCCTTCTT+CGG 0.450058 3.2:+76112024 MS.gene02497:CDS
AAGTTAAGTTGTACTGTGAT+AGG 0.450787 3.2:+76112842 MS.gene02497:CDS
CCAAAGAATTGCAGCCTACA+TGG 0.451697 3.2:+76111943 MS.gene02497:CDS
GAGATCAAAACTAGAAGCAT+TGG 0.459866 3.2:+76111583 MS.gene02497:CDS
ACTTGATTGTTGCTTCAGCT+TGG 0.468732 3.2:+76112917 MS.gene02497:CDS
TCTGGAGACGAATACTCCTT+TGG 0.473491 3.2:-76111749 None:intergenic
TTCGGATCAGATTTGGTGAT+GGG 0.474390 3.2:+76111521 MS.gene02497:CDS
TGATTCAAACACTTGCTTCG+AGG 0.476019 3.2:+76112203 MS.gene02497:CDS
TACTCTTCCTAGAGAGAGCC+AGG 0.477900 3.2:+76112642 MS.gene02497:CDS
AGCATATTCTGAATTGGATC+AGG 0.480387 3.2:-76111704 None:intergenic
CGTCAGGCATGTGATGAAGC+TGG 0.484030 3.2:-76112447 None:intergenic
TTGCATGTGAAGGTGAAGAT+AGG 0.487421 3.2:+76112716 MS.gene02497:CDS
TCTCAACATTCATCCTATCC+TGG 0.491515 3.2:-76112660 None:intergenic
GTTGGAGGACTAAAGATCAT+AGG 0.492091 3.2:+76112289 MS.gene02497:CDS
AGCGATGCAGATACTGTTCG+AGG 0.503528 3.2:+76112057 MS.gene02497:CDS
ACGAGACCAACTTCTTCGCT+TGG 0.503960 3.2:+76112492 MS.gene02497:CDS
TGATTGTTGCTTCAGCTTGG+AGG 0.503995 3.2:+76112920 MS.gene02497:CDS
ACATTCATAAAGAAGTTGCT+TGG 0.509000 3.2:-76111831 None:intergenic
ATCCTATCCTGGCTCTCTCT+AGG 0.512100 3.2:-76112649 None:intergenic
TCTTTACCAAGCGAAGAAGT+TGG 0.520115 3.2:-76112498 None:intergenic
TCGCGGGAAAATGGAGAGCT+AGG 0.521293 3.2:+76112755 MS.gene02497:CDS
GCTGCTAGACGGTCCGAAGA+AGG 0.532544 3.2:-76112037 None:intergenic
TCTGTGCAGCGATCCGTTGG+AGG 0.533052 3.2:+76112274 MS.gene02497:CDS
CGAAGCAAGTGTTTGAATCA+GGG 0.537450 3.2:-76112200 None:intergenic
GTAGAGATGTCAGAACTAGA+TGG 0.537559 3.2:-76111455 None:intergenic
GAGTCTGTGCAGCGATCCGT+TGG 0.538684 3.2:+76112271 MS.gene02497:CDS
TCCAAAACTTGTAACGGTCG+TGG 0.541218 3.2:+76112528 MS.gene02497:CDS
TTATAGAGCTTTGAAATGCA+AGG 0.546542 3.2:+76112009 MS.gene02497:CDS
TACAAGATAAAGGAGGAAAA+GGG 0.547340 3.2:+76112865 MS.gene02497:CDS
AGAGCTAGGATGACAATGGC+TGG 0.548519 3.2:+76112769 MS.gene02497:CDS
TTTGAATCCAAAACTTGTAA+CGG 0.552516 3.2:+76112522 MS.gene02497:CDS
TAGTGACATTGAGGAAGAAG+AGG 0.556415 3.2:+76111625 MS.gene02497:CDS
ACGGATCGCTGCACAGACTC+AGG 0.557758 3.2:-76112268 None:intergenic
GAACAACTAGCAGAAACACT+TGG 0.558635 3.2:+76112322 MS.gene02497:CDS
ACTTGATGATAGTGACATTG+AGG 0.558721 3.2:+76111616 MS.gene02497:CDS
TTTCGGATCAGATTTGGTGA+TGG 0.559466 3.2:+76111520 MS.gene02497:CDS
ATATGGTAATTCTCAAAGCA+TGG 0.562265 3.2:+76111649 MS.gene02497:CDS
CCGGTCAATCTAACATGTGA+TGG 0.566500 3.2:-76112235 None:intergenic
CATCACATGTTAGATTGACC+GGG 0.567991 3.2:+76112236 MS.gene02497:CDS
CTCGTAACTCTCGCTCGAGT+AGG 0.572630 3.2:-76111393 None:intergenic
TTGAGAGGCAATGCTTGGCA+AGG 0.577561 3.2:+76112677 MS.gene02497:CDS
AATTGCAGCCTACATGGTGG+AGG 0.577669 3.2:+76111949 MS.gene02497:CDS
TAGGCTGCAATTCTTTGGGA+AGG 0.583102 3.2:-76111938 None:intergenic
CAAAACTAGAAGCATTGGAG+AGG 0.583413 3.2:+76111588 MS.gene02497:CDS
GAATGTTGAGAGGCAATGCT+TGG 0.583862 3.2:+76112672 MS.gene02497:CDS
CTACAAGTTTCATGCCAACA+AGG 0.584293 3.2:+76111474 MS.gene02497:CDS
TGTGCTACTGCAATTTCAGA+AGG 0.584377 3.2:+76111851 MS.gene02497:CDS
GCCTGACGAGAGCGTTTCAA+CGG 0.590858 3.2:+76112462 MS.gene02497:CDS
TCCGGATTCAGCGAGGCGAG+CGG 0.592435 3.2:-76111978 None:intergenic
AATACTCCTTTGGTGAGTCA+TGG 0.604923 3.2:-76111739 None:intergenic
CACTAAGCTCAAATCGCCAA+AGG 0.608012 3.2:+76111295 MS.gene02497:CDS
TATGATCTTTAGTCCTCCAA+CGG 0.609253 3.2:-76112287 None:intergenic
GTATCTGCATCGCTGCTAGA+CGG 0.609923 3.2:-76112048 None:intergenic
ATTCATAAAGAAGTTGCTTG+GGG 0.610084 3.2:-76111829 None:intergenic
GTTAATACGGTTGCATGTGA+AGG 0.612068 3.2:+76112706 MS.gene02497:CDS
ATAGAACGGTATGAAGTCGC+GGG 0.612894 3.2:+76112739 MS.gene02497:CDS
AGACTCAGGATCATCAATCC+CGG 0.614792 3.2:-76112254 None:intergenic
TAACTATGCAATAACAGAAG+CGG 0.616342 3.2:+76112114 MS.gene02497:CDS
GATAGAACGGTATGAAGTCG+CGG 0.616637 3.2:+76112738 MS.gene02497:CDS
TCGTCGTGGAGAAGCTCTTG+TGG 0.619304 3.2:+76112411 MS.gene02497:CDS
CCTTTGGTGAGTCATGGTCA+TGG 0.621918 3.2:-76111733 None:intergenic
AAGGTGAAGATAGGATAGAA+CGG 0.625100 3.2:+76112725 MS.gene02497:CDS
TTCCTAGAGAGAGCCAGGAT+AGG 0.625909 3.2:+76112647 MS.gene02497:CDS
TGAGAGGCAATGCTTGGCAA+GGG 0.635494 3.2:+76112678 MS.gene02497:CDS
TCGATGCTTGATTGTCGTCG+TGG 0.642078 3.2:+76112397 MS.gene02497:CDS
AAAACTAGAAGCATTGGAGA+GGG 0.649054 3.2:+76111589 MS.gene02497:CDS
AGAAGATCCAATTAATGACA+TGG 0.650375 3.2:-76111246 None:intergenic
ATTGCAGCCTACATGGTGGA+GGG 0.650477 3.2:+76111950 MS.gene02497:CDS
GGCGAGTCCCTCCACCATGT+AGG 0.652264 3.2:-76111957 None:intergenic
GCTAGACGGTCCGAAGAAGG+AGG 0.655079 3.2:-76112034 None:intergenic
TTCATAAAGAAGTTGCTTGG+GGG 0.656482 3.2:-76111828 None:intergenic
ATGAGAATAAGAAATTTGGG+AGG 0.658872 3.2:-76111342 None:intergenic
AGGATAGGATGAATGTTGAG+AGG 0.660140 3.2:+76112662 MS.gene02497:CDS
AATTTCAGAAGGAAATGAAG+AGG 0.662867 3.2:+76111862 MS.gene02497:CDS
ATGGAGAGCTAGGATGACAA+TGG 0.667953 3.2:+76112765 MS.gene02497:CDS
CCATGACCATGACTCACCAA+AGG 0.671972 3.2:+76111733 MS.gene02497:CDS
CTTGAATTGAGACAATTCGT+CGG 0.672967 3.2:-76111910 None:intergenic
AATTTCGAGCGGTCTCATCG+AGG 0.679065 3.2:+76112356 MS.gene02497:CDS
TTTGTCATTTGAATTTCGAG+CGG 0.680630 3.2:+76112345 MS.gene02497:CDS
AAGAATTGCAGCCTACATGG+TGG 0.682524 3.2:+76111946 MS.gene02497:CDS
TGATAGGTACAAGATAAAGG+AGG 0.692576 3.2:+76112858 MS.gene02497:CDS
ACGACAATCAAGCATCGATG+AGG 0.692866 3.2:-76112392 None:intergenic
ATAAACCCGAATTTAAAACA+CGG 0.716869 3.2:-76112085 None:intergenic
TTCATGCCAACAAGGCAACG+TGG 0.722675 3.2:+76111482 MS.gene02497:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAATAACGAAAATTTCT+AGG + chr3.2:76111804-76111823 MS.gene02497:CDS 20.0%
!! CTTATGAGAATAAGAAATTT+GGG - chr3.2:76111348-76111367 None:intergenic 20.0%
!!! AGAAATTTTCGTTATTTCTT+TGG - chr3.2:76111804-76111823 None:intergenic 20.0%
!!! ATAGATTTTGATATCAATCA+AGG + chr3.2:76112163-76112182 MS.gene02497:CDS 20.0%
! GCTTATGAGAATAAGAAATT+TGG - chr3.2:76111349-76111368 None:intergenic 25.0%
! TTGATCCTAACATTATTGAT+TGG + chr3.2:76111675-76111694 MS.gene02497:CDS 25.0%
! TTTGAATCCAAAACTTGTAA+CGG + chr3.2:76112522-76112541 MS.gene02497:CDS 25.0%
!! ATAAACCCGAATTTAAAACA+CGG - chr3.2:76112088-76112107 None:intergenic 25.0%
!! TGATCCTAACATTATTGATT+GGG + chr3.2:76111676-76111695 MS.gene02497:CDS 25.0%
!!! AATTTTCGTTATTTCTTTGG+TGG - chr3.2:76111801-76111820 None:intergenic 25.0%
!!! AGCTCTATAAATACTTTTTC+CGG - chr3.2:76111999-76112018 None:intergenic 25.0%
!!! AGTACAACTTAACTTGTTTT+CGG - chr3.2:76112837-76112856 None:intergenic 25.0%
AAATTTGGGAGGAAAAATCA+GGG - chr3.2:76111334-76111353 None:intergenic 30.0%
AATTTCAGAAGGAAATGAAG+AGG + chr3.2:76111862-76111881 MS.gene02497:CDS 30.0%
ACATTCATAAAGAAGTTGCT+TGG - chr3.2:76111834-76111853 None:intergenic 30.0%
ATATGGTAATTCTCAAAGCA+TGG + chr3.2:76111649-76111668 MS.gene02497:CDS 30.0%
ATGAGAATAAGAAATTTGGG+AGG - chr3.2:76111345-76111364 None:intergenic 30.0%
ATTCATAAAGAAGTTGCTTG+GGG - chr3.2:76111832-76111851 None:intergenic 30.0%
CATTCATAAAGAAGTTGCTT+GGG - chr3.2:76111833-76111852 None:intergenic 30.0%
GTGAATTCAATTGAAGTTGA+TGG + chr3.2:76111554-76111573 MS.gene02497:CDS 30.0%
TAACTATGCAATAACAGAAG+CGG + chr3.2:76112114-76112133 MS.gene02497:CDS 30.0%
TACAAGATAAAGGAGGAAAA+GGG + chr3.2:76112865-76112884 MS.gene02497:CDS 30.0%
TCAACAAATCTCTGCAAAAA+CGG - chr3.2:76112580-76112599 None:intergenic 30.0%
! AAGTTAAGTTGTACTGTGAT+AGG + chr3.2:76112842-76112861 MS.gene02497:CDS 30.0%
! TTTGTCATTTGAATTTCGAG+CGG + chr3.2:76112345-76112364 MS.gene02497:CDS 30.0%
!! GTTTGTCCGTGTTTTAAATT+CGG + chr3.2:76112079-76112098 MS.gene02497:CDS 30.0%
!! TTATAGAGCTTTGAAATGCA+AGG + chr3.2:76112009-76112028 MS.gene02497:CDS 30.0%
!! TTTGTCCGTGTTTTAAATTC+GGG + chr3.2:76112080-76112099 MS.gene02497:CDS 30.0%
AACAAATCTCTGCAAAAACG+GGG - chr3.2:76112578-76112597 None:intergenic 35.0%
AACAGCAGCATATTCTGAAT+TGG - chr3.2:76111713-76111732 None:intergenic 35.0%
AAGGTGAAGATAGGATAGAA+CGG + chr3.2:76112725-76112744 MS.gene02497:CDS 35.0%
AGCATATTCTGAATTGGATC+AGG - chr3.2:76111707-76111726 None:intergenic 35.0%
ATGCTGCTAATATTGGAATC+TGG - chr3.2:76111770-76111789 None:intergenic 35.0%
ATTGAGGAAGAAGAGGAATA+TGG + chr3.2:76111632-76111651 MS.gene02497:CDS 35.0%
CAACAAATCTCTGCAAAAAC+GGG - chr3.2:76112579-76112598 None:intergenic 35.0%
CTGTGATAGGTACAAGATAA+AGG + chr3.2:76112855-76112874 MS.gene02497:CDS 35.0%
CTTGAATTGAGACAATTCGT+CGG - chr3.2:76111913-76111932 None:intergenic 35.0%
GAAATTTGGGAGGAAAAATC+AGG - chr3.2:76111335-76111354 None:intergenic 35.0%
GGCAAGGGATATTGTTAATA+CGG + chr3.2:76112693-76112712 MS.gene02497:CDS 35.0%
GTACAAGATAAAGGAGGAAA+AGG + chr3.2:76112864-76112883 MS.gene02497:CDS 35.0%
TATGATCTTTAGTCCTCCAA+CGG - chr3.2:76112290-76112309 None:intergenic 35.0%
TCAGCCCAATCAATAATGTT+AGG - chr3.2:76111683-76111702 None:intergenic 35.0%
TGATAGGTACAAGATAAAGG+AGG + chr3.2:76112858-76112877 MS.gene02497:CDS 35.0%
TTCATAAAGAAGTTGCTTGG+GGG - chr3.2:76111831-76111850 None:intergenic 35.0%
! ACTTGATGATAGTGACATTG+AGG + chr3.2:76111616-76111635 MS.gene02497:CDS 35.0%
! AGCTTAGTGCTTTTATGTGA+TGG - chr3.2:76111284-76111303 None:intergenic 35.0%
! GAGATCAAAACTAGAAGCAT+TGG + chr3.2:76111583-76111602 MS.gene02497:CDS 35.0%
! TTAGTGCTTTTATGTGATGG+TGG - chr3.2:76111281-76111300 None:intergenic 35.0%
!! AAAACTAGAAGCATTGGAGA+GGG + chr3.2:76111589-76111608 MS.gene02497:CDS 35.0%
AATACTCCTTTGGTGAGTCA+TGG - chr3.2:76111742-76111761 None:intergenic 40.0%
AGGATAGGATGAATGTTGAG+AGG + chr3.2:76112662-76112681 MS.gene02497:CDS 40.0%
CACCAAATCTGATCCGAAAT+TGG - chr3.2:76111519-76111538 None:intergenic 40.0%
CATCACATGTTAGATTGACC+GGG + chr3.2:76112236-76112255 MS.gene02497:CDS 40.0%
CATGTAGGCTGCAATTCTTT+GGG - chr3.2:76111945-76111964 None:intergenic 40.0%
CCATCACATGTTAGATTGAC+CGG + chr3.2:76112235-76112254 MS.gene02497:CDS 40.0%
CGAAGCAAGTGTTTGAATCA+GGG - chr3.2:76112203-76112222 None:intergenic 40.0%
CGACGAATTGTCTCAATTCA+AGG + chr3.2:76111911-76111930 MS.gene02497:CDS 40.0%
CTACAAGTTTCATGCCAACA+AGG + chr3.2:76111474-76111493 MS.gene02497:CDS 40.0%
GAACAACTAGCAGAAACACT+TGG + chr3.2:76112322-76112341 MS.gene02497:CDS 40.0%
GCTACTGATGCTGCTAATAT+TGG - chr3.2:76111777-76111796 None:intergenic 40.0%
GTAGAGATGTCAGAACTAGA+TGG - chr3.2:76111458-76111477 None:intergenic 40.0%
GTCCAATTTCGGATCAGATT+TGG + chr3.2:76111514-76111533 MS.gene02497:CDS 40.0%
GTTAATACGGTTGCATGTGA+AGG + chr3.2:76112706-76112725 MS.gene02497:CDS 40.0%
GTTGGAGGACTAAAGATCAT+AGG + chr3.2:76112289-76112308 MS.gene02497:CDS 40.0%
TAGTGACATTGAGGAAGAAG+AGG + chr3.2:76111625-76111644 MS.gene02497:CDS 40.0%
TCACTCACATTTGTGCTCAT+TGG - chr3.2:76112808-76112827 None:intergenic 40.0%
TCGAAGCAAGTGTTTGAATC+AGG - chr3.2:76112204-76112223 None:intergenic 40.0%
TCTCAACATTCATCCTATCC+TGG - chr3.2:76112663-76112682 None:intergenic 40.0%
TCTTTACCAAGCGAAGAAGT+TGG - chr3.2:76112501-76112520 None:intergenic 40.0%
TGATTCAAACACTTGCTTCG+AGG + chr3.2:76112203-76112222 MS.gene02497:CDS 40.0%
TTGCATGTGAAGGTGAAGAT+AGG + chr3.2:76112716-76112735 MS.gene02497:CDS 40.0%
! ACTTGATTGTTGCTTCAGCT+TGG + chr3.2:76112917-76112936 MS.gene02497:CDS 40.0%
! TGTGCTACTGCAATTTCAGA+AGG + chr3.2:76111851-76111870 MS.gene02497:CDS 40.0%
!! CAAAACTAGAAGCATTGGAG+AGG + chr3.2:76111588-76111607 MS.gene02497:CDS 40.0%
!! GAAAAGGGTGCACTTCATTT+TGG + chr3.2:76112880-76112899 MS.gene02497:CDS 40.0%
!! GGGTTAACATCGATTTCCTT+TGG - chr3.2:76111314-76111333 None:intergenic 40.0%
!! TTCGGATCAGATTTGGTGAT+GGG + chr3.2:76111521-76111540 MS.gene02497:CDS 40.0%
!! TTTCGGATCAGATTTGGTGA+TGG + chr3.2:76111520-76111539 MS.gene02497:CDS 40.0%
AAATCTCTGCAAAAACGGGG+CGG - chr3.2:76112575-76112594 None:intergenic 45.0%
AAGAATTGCAGCCTACATGG+TGG + chr3.2:76111946-76111965 MS.gene02497:CDS 45.0%
AGACTCAGGATCATCAATCC+CGG - chr3.2:76112257-76112276 None:intergenic 45.0%
ATAGAACGGTATGAAGTCGC+GGG + chr3.2:76112739-76112758 MS.gene02497:CDS 45.0%
ATGCAAGGAACCTCCTTCTT+CGG + chr3.2:76112024-76112043 MS.gene02497:CDS 45.0%
ATGGAGAGCTAGGATGACAA+TGG + chr3.2:76112765-76112784 MS.gene02497:CDS 45.0%
CACTAAGCTCAAATCGCCAA+AGG + chr3.2:76111295-76111314 MS.gene02497:CDS 45.0%
CCAAAGAATTGCAGCCTACA+TGG + chr3.2:76111943-76111962 MS.gene02497:CDS 45.0%
CCATGTAGGCTGCAATTCTT+TGG - chr3.2:76111946-76111965 None:intergenic 45.0%
CCGGTCAATCTAACATGTGA+TGG - chr3.2:76112238-76112257 None:intergenic 45.0%
GAATGTTGAGAGGCAATGCT+TGG + chr3.2:76112672-76112691 MS.gene02497:CDS 45.0%
GATAGAACGGTATGAAGTCG+CGG + chr3.2:76112738-76112757 MS.gene02497:CDS 45.0%
TCCAAAACTTGTAACGGTCG+TGG + chr3.2:76112528-76112547 MS.gene02497:CDS 45.0%
TCTGGAGACGAATACTCCTT+TGG - chr3.2:76111752-76111771 None:intergenic 45.0%
! TAGGCTGCAATTCTTTGGGA+AGG - chr3.2:76111941-76111960 None:intergenic 45.0%
! TCCACGACCGTTACAAGTTT+TGG - chr3.2:76112532-76112551 None:intergenic 45.0%
! TGATTGTTGCTTCAGCTTGG+AGG + chr3.2:76112920-76112939 MS.gene02497:CDS 45.0%
!! ACGACAATCAAGCATCGATG+AGG - chr3.2:76112395-76112414 None:intergenic 45.0%
!! AGGGTGCACTTCATTTTGGT+TGG + chr3.2:76112884-76112903 MS.gene02497:CDS 45.0%
!! GGTGCTGATCAGTCCAATTT+CGG + chr3.2:76111503-76111522 MS.gene02497:CDS 45.0%
!!! GGGTGCACTTCATTTTGGTT+GGG + chr3.2:76112885-76112904 MS.gene02497:CDS 45.0%
!!! TACTTTTTCCGGATTCAGCG+AGG - chr3.2:76111988-76112007 None:intergenic 45.0%
AATTGCAGCCTACATGGTGG+AGG + chr3.2:76111949-76111968 MS.gene02497:CDS 50.0%
AATTTCGAGCGGTCTCATCG+AGG + chr3.2:76112356-76112375 MS.gene02497:CDS 50.0%
ACGAGACCAACTTCTTCGCT+TGG + chr3.2:76112492-76112511 MS.gene02497:CDS 50.0%
AGAGCTAGGATGACAATGGC+TGG + chr3.2:76112769-76112788 MS.gene02497:CDS 50.0%
AGCGATGCAGATACTGTTCG+AGG + chr3.2:76112057-76112076 MS.gene02497:CDS 50.0%
CCATGACCATGACTCACCAA+AGG + chr3.2:76111733-76111752 MS.gene02497:CDS 50.0%
CCTTTGGTGAGTCATGGTCA+TGG - chr3.2:76111736-76111755 None:intergenic 50.0%
GGTATGAAGTCGCGGGAAAA+TGG + chr3.2:76112746-76112765 MS.gene02497:CDS 50.0%
GTATCTGCATCGCTGCTAGA+CGG - chr3.2:76112051-76112070 None:intergenic 50.0%
TACTCTTCCTAGAGAGAGCC+AGG + chr3.2:76112642-76112661 MS.gene02497:CDS 50.0%
TCGATGCTTGATTGTCGTCG+TGG + chr3.2:76112397-76112416 MS.gene02497:CDS 50.0%
TGAGAGGCAATGCTTGGCAA+GGG + chr3.2:76112678-76112697 MS.gene02497:CDS 50.0%
TTCATGCCAACAAGGCAACG+TGG + chr3.2:76111482-76111501 MS.gene02497:CDS 50.0%
TTCCTAGAGAGAGCCAGGAT+AGG + chr3.2:76112647-76112666 MS.gene02497:CDS 50.0%
TTGAGAGGCAATGCTTGGCA+AGG + chr3.2:76112677-76112696 MS.gene02497:CDS 50.0%
! ATCCTATCCTGGCTCTCTCT+AGG - chr3.2:76112652-76112671 None:intergenic 50.0%
! ATTGCAGCCTACATGGTGGA+GGG + chr3.2:76111950-76111969 MS.gene02497:CDS 50.0%
ACCGTTGAAACGCTCTCGTC+AGG - chr3.2:76112466-76112485 None:intergenic 55.0%
CGTCAGGCATGTGATGAAGC+TGG - chr3.2:76112450-76112469 None:intergenic 55.0%
CTCGTAACTCTCGCTCGAGT+AGG - chr3.2:76111396-76111415 None:intergenic 55.0%
CTGCAAAAACGGGGCGGTAT+TGG - chr3.2:76112569-76112588 None:intergenic 55.0%
TCAGCACCACGTTGCCTTGT+TGG - chr3.2:76111491-76111510 None:intergenic 55.0%
TCGCGGGAAAATGGAGAGCT+AGG + chr3.2:76112755-76112774 MS.gene02497:CDS 55.0%
TCGTCGTGGAGAAGCTCTTG+TGG + chr3.2:76112411-76112430 MS.gene02497:CDS 55.0%
!! GCCTGACGAGAGCGTTTCAA+CGG + chr3.2:76112462-76112481 MS.gene02497:CDS 55.0%
ACGGATCGCTGCACAGACTC+AGG - chr3.2:76112271-76112290 None:intergenic 60.0%
GAGTCTGTGCAGCGATCCGT+TGG + chr3.2:76112271-76112290 MS.gene02497:CDS 60.0%
GCTAGACGGTCCGAAGAAGG+AGG - chr3.2:76112037-76112056 None:intergenic 60.0%
GCTGCTAGACGGTCCGAAGA+AGG - chr3.2:76112040-76112059 None:intergenic 60.0%
TCTGTGCAGCGATCCGTTGG+AGG + chr3.2:76112274-76112293 MS.gene02497:CDS 60.0%
GGCGAGTCCCTCCACCATGT+AGG - chr3.2:76111960-76111979 None:intergenic 65.0%
TCCGGATTCAGCGAGGCGAG+CGG - chr3.2:76111981-76112000 None:intergenic 65.0%
GCCGCTCGCCTCGCTGAATC+CGG + chr3.2:76111977-76111996 MS.gene02497:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 76111248 76112945 76111248 ID=MS.gene02497
chr3.2 mRNA 76111248 76112945 76111248 ID=MS.gene02497.t1;Parent=MS.gene02497
chr3.2 exon 76111248 76112945 76111248 ID=MS.gene02497.t1.exon1;Parent=MS.gene02497.t1
chr3.2 CDS 76111248 76112945 76111248 ID=cds.MS.gene02497.t1;Parent=MS.gene02497.t1
Gene Sequence

>MS.gene02497

ATGTCATTAATTGGATCTTCTGAGCTAGCTCCACCATCACATAAAAGCACTAAGCTCAAATCGCCAAAGGAAATCGATGTTAACCCTGATTTTTCCTCCCAAATTTCTTATTCTCATAAGCATAGAAGCATGTATATCACAGATACCTACTCGAGCGAGAGTTACGAGAAGTACTTCAATGACTCACAGACAGAAGAACTAATAGAACCATCTAGTTCTGACATCTCTACAAGTTTCATGCCAACAAGGCAACGTGGTGCTGATCAGTCCAATTTCGGATCAGATTTGGTGATGGGAAATCGAAGCGTGAATTCAATTGAAGTTGATGGTGCAATGAGATCAAAACTAGAAGCATTGGAGAGGGCATTACTTGATGATAGTGACATTGAGGAAGAAGAGGAATATGGTAATTCTCAAAGCATGGAAATTGATCCTAACATTATTGATTGGGCTGATCCTGATCCAATTCAGAATATGCTGCTGTTCCATGACCATGACTCACCAAAGGAGTATTCGTCTCCAGATTCCAATATTAGCAGCATCAGTAGCACCACCAAAGAAATAACGAAAATTTCTAGGACCCCCAAGCAACTTCTTTATGAATGTGCTACTGCAATTTCAGAAGGAAATGAAGAGGAAGCATCGTCGATGATAAACAATCTCCGACGAATTGTCTCAATTCAAGGAGAGCCTTCCCAAAGAATTGCAGCCTACATGGTGGAGGGACTCGCCGCTCGCCTCGCTGAATCCGGAAAAAGTATTTATAGAGCTTTGAAATGCAAGGAACCTCCTTCTTCGGACCGTCTAGCAGCGATGCAGATACTGTTCGAGGTTTGTCCGTGTTTTAAATTCGGGTTTATTGCAGCTAACTATGCAATAACAGAAGCGGTTAAAGATGAAATGAAGATTCATATAATAGATTTTGATATCAATCAAGGAAGTCAGTACATAACCCTGATTCAAACACTTGCTTCGAGGTCTTGTAAGCCATCACATGTTAGATTGACCGGGATTGATGATCCTGAGTCTGTGCAGCGATCCGTTGGAGGACTAAAGATCATAGGACAAAGACTTGAACAACTAGCAGAAACACTTGGTTTGTCATTTGAATTTCGAGCGGTCTCATCGAGGACTTCAATTGTCACCTCATCGATGCTTGATTGTCGTCGTGGAGAAGCTCTTGTGGTGAACTTTGCATTCCAGCTTCATCACATGCCTGACGAGAGCGTTTCAACGGTGAACGAACGAGACCAACTTCTTCGCTTGGTAAAGAGTTTGAATCCAAAACTTGTAACGGTCGTGGAGCAAGATGTAAACACCAATACCGCCCCGTTTTTGCAGAGATTTGTTGAAGCTTACAATTACTACTCTGCTGTGTTTGAGTCACTTGATGTTACTCTTCCTAGAGAGAGCCAGGATAGGATGAATGTTGAGAGGCAATGCTTGGCAAGGGATATTGTTAATACGGTTGCATGTGAAGGTGAAGATAGGATAGAACGGTATGAAGTCGCGGGAAAATGGAGAGCTAGGATGACAATGGCTGGATTCAGTTCATCACCAATGAGCACAAATGTGAGTGATGAAATCCGAAAACAAGTTAAGTTGTACTGTGATAGGTACAAGATAAAGGAGGAAAAGGGTGCACTTCATTTTGGTTGGGAAGACAAAAACTTGATTGTTGCTTCAGCTTGGAGGTGA
Protein sequence

>MS.gene02497.t1

MSLIGSSELAPPSHKSTKLKSPKEIDVNPDFSSQISYSHKHRSMYITDTYSSESYEKYFNDSQTEELIEPSSSDISTSFMPTRQRGADQSNFGSDLVMGNRSVNSIEVDGAMRSKLEALERALLDDSDIEEEEEYGNSQSMEIDPNIIDWADPDPIQNMLLFHDHDSPKEYSSPDSNISSISSTTKEITKISRTPKQLLYECATAISEGNEEEASSMINNLRRIVSIQGEPSQRIAAYMVEGLAARLAESGKSIYRALKCKEPPSSDRLAAMQILFEVCPCFKFGFIAANYAITEAVKDEMKIHIIDFDINQGSQYITLIQTLASRSCKPSHVRLTGIDDPESVQRSVGGLKIIGQRLEQLAETLGLSFEFRAVSSRTSIVTSSMLDCRRGEALVVNFAFQLHHMPDESVSTVNERDQLLRLVKSLNPKLVTVVEQDVNTNTAPFLQRFVEAYNYYSAVFESLDVTLPRESQDRMNVERQCLARDIVNTVACEGEDRIERYEVAGKWRARMTMAGFSSSPMSTNVSDEIRKQVKLYCDRYKIKEEKGALHFGWEDKNLIVASAWR