AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025132

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025132.t1 MTR_2g039880 95.719 584 25 0 1 584 1 584 0.0 1100
MS.gene025132.t1 MTR_2g028950 44.234 529 268 9 46 556 45 564 1.63e-142 425
MS.gene025132.t1 MTR_5g090770 40.150 533 293 10 46 561 48 571 3.27e-122 373
MS.gene025132.t1 MTR_2g028950 43.656 465 236 8 46 492 45 501 3.39e-120 365
MS.gene025132.t1 MTR_5g090770 41.463 410 217 8 46 440 48 449 2.90e-94 296
MS.gene025132.t1 MTR_1g051025 31.129 363 207 8 74 400 32 387 2.38e-38 148
MS.gene025132.t1 MTR_7g090020 30.376 372 216 7 65 400 24 388 8.26e-36 140
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025132.t1 AT1G64280 52.021 569 242 9 7 556 7 563 0.0 550
MS.gene025132.t1 AT4G26120 49.912 571 258 10 12 567 16 573 3.14e-174 507
MS.gene025132.t1 AT4G26120 49.912 571 258 10 12 567 15 572 3.31e-174 507
MS.gene025132.t1 AT5G45110 41.825 526 282 9 46 554 41 559 6.19e-121 370
MS.gene025132.t1 AT4G19660 40.437 549 284 12 29 553 22 551 1.36e-120 369
MS.gene025132.t1 AT4G19660 38.715 576 283 13 29 553 22 578 1.96e-115 356
MS.gene025132.t1 AT5G45110 40.956 481 260 9 46 512 41 511 9.97e-107 331
MS.gene025132.t1 AT3G57130 32.095 377 199 12 65 400 26 386 3.60e-39 150
MS.gene025132.t1 AT2G41370 30.159 378 213 9 65 400 25 393 1.10e-34 137

Find 99 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTGAATTTACTCCGAATT+TGG 0.080716 2.4:+50181030 MS.gene025132:CDS
ACTACTGAGCTTCTTGATTT+TGG 0.125114 2.4:+50180452 MS.gene025132:CDS
GGTGAGGAGTAGTTTCTTTA+AGG 0.187941 2.4:+50179510 MS.gene025132:CDS
TATATTATTTGTTTCACTAT+AGG 0.288345 2.4:-50180239 None:intergenic
GCGCTTCACTTGTCGATCTT+CGG 0.290085 2.4:-50181521 None:intergenic
ACTAGACATTCTTGACAAAA+TGG 0.299539 2.4:+50180079 MS.gene025132:CDS
AGAATTGCTTGCTCTTTATC+AGG 0.305066 2.4:+50179777 MS.gene025132:CDS
ATGTTTGTCTAGAAGATTTA+CGG 0.318420 2.4:-50180297 None:intergenic
CGCTTCACTTGTCGATCTTC+GGG 0.329375 2.4:-50181520 None:intergenic
GCTACAAGAAGTTCTTAATA+AGG 0.338445 2.4:+50181558 MS.gene025132:CDS
ATCTAAAGCTCTGTGTATTC+TGG 0.354720 2.4:-50180324 None:intergenic
GAAGTAGTAATAGTTGAATT+TGG 0.361003 2.4:-50179337 None:intergenic
TCGATTGTGTATCGAGATAT+TGG 0.362910 2.4:+50180694 MS.gene025132:CDS
TCTGAATTTACTCCGAATTT+GGG 0.369283 2.4:+50181031 MS.gene025132:CDS
AATTCAGATGTGCCATCAAT+TGG 0.381830 2.4:-50181016 None:intergenic
AAAGAGGAGTTTGATAAATC+TGG 0.386748 2.4:+50181595 MS.gene025132:CDS
GTGACTAAGGATTATGATGT+TGG 0.390092 2.4:+50179577 MS.gene025132:CDS
TCATCTTGAGCAGTGGAGTT+GGG 0.392046 2.4:+50181406 MS.gene025132:intron
ATTGTTTACACTTTGGTTGT+TGG 0.395598 2.4:+50179698 MS.gene025132:CDS
TCATCCTCTTCCACGTCAAT+AGG 0.396489 2.4:+50181628 MS.gene025132:CDS
CTCTGGCAACCACCCAAATT+CGG 0.404385 2.4:-50181043 None:intergenic
GCTTCAATACTAGATGATAC+AGG 0.421643 2.4:+50179412 MS.gene025132:CDS
CAAACTCCTCTTTGTCCTCA+TGG 0.421934 2.4:-50181585 None:intergenic
TAAAAGAGGAGCATCTAATT+CGG 0.429046 2.4:+50181101 MS.gene025132:CDS
GGAAACTTCTCGACCATCTC+TGG 0.429174 2.4:-50179467 None:intergenic
TCTCTTTCTCTTGCTAAGGC+AGG 0.429539 2.4:+50180755 MS.gene025132:CDS
CAGATGATGTAGAATTAGTA+AGG 0.438766 2.4:+50180348 MS.gene025132:CDS
CTCTTTCTCTTGCTAAGGCA+GGG 0.442710 2.4:+50180756 MS.gene025132:CDS
GTCATCTTGAGCAGTGGAGT+TGG 0.451507 2.4:+50181405 MS.gene025132:intron
GTTCTGCGTTATTTGTATAG+TGG 0.456294 2.4:+50179619 MS.gene025132:CDS
ATCATACTATTATTAGCCCT+TGG 0.460763 2.4:-50181655 None:intergenic
CCTATCGCAGCTCGCATGCA+TGG 0.465300 2.4:+50181486 MS.gene025132:CDS
GGCATCTCTTTCTCTTGCTA+AGG 0.467473 2.4:+50180751 MS.gene025132:CDS
ATGGATATCAATCCAATTGA+TGG 0.475189 2.4:+50181004 MS.gene025132:CDS
TGACTAAGGATTATGATGTT+GGG 0.476464 2.4:+50179578 MS.gene025132:CDS
CTTTCTATAGCAAACATTTG+TGG 0.480797 2.4:+50180125 MS.gene025132:CDS
ACGGTTCTTCCCTCGTTGCT+CGG 0.483600 2.4:+50181432 MS.gene025132:CDS
TGAAGCATGAAATCAACAAC+AGG 0.485589 2.4:-50179721 None:intergenic
GTCGAGAAGTTTCCGTGCAC+CGG 0.497677 2.4:+50179476 MS.gene025132:CDS
TGGTTGCCAGAGGACAACTA+TGG 0.504234 2.4:+50181054 MS.gene025132:CDS
AGAAACGACGCTGCAACTAA+AGG 0.505993 2.4:+50179552 MS.gene025132:CDS
CATTCCTATTGACGTGGAAG+AGG 0.507555 2.4:-50181632 None:intergenic
ATCACAACCTTTGCTTCCAT+CGG 0.509584 2.4:-50180983 None:intergenic
TCTGGCGATGATCTTTGAAT+CGG 0.509702 2.4:-50179449 None:intergenic
GAGCAGTGGAGTTGGGGAAA+CGG 0.514130 2.4:+50181413 MS.gene025132:intron
GCAACTAAAGGAAGTGACTA+AGG 0.518727 2.4:+50179564 MS.gene025132:CDS
TCTTTCTCTTGCTAAGGCAG+GGG 0.519191 2.4:+50180757 MS.gene025132:CDS
TCAAAGATCATCGCCAGAGA+TGG 0.520757 2.4:+50179454 MS.gene025132:CDS
ATTGGAGCAAGCTGAGAGAA+GGG 0.524671 2.4:+50180712 MS.gene025132:CDS
GATGATGATTGTTTACACTT+TGG 0.524715 2.4:+50179691 MS.gene025132:CDS
ATTGTATCTTTGTTAACGAA+GGG 0.525145 2.4:+50180554 MS.gene025132:CDS
TCAAGTCCATAGTTGTCCTC+TGG 0.532702 2.4:-50181060 None:intergenic
GGAAGTGTTGAACAAGATCA+TGG 0.534097 2.4:+50181453 MS.gene025132:CDS
CTATCGCAGCTCGCATGCAT+GGG 0.540766 2.4:+50181487 MS.gene025132:CDS
GTAATGATGTACTTGAGAAG+TGG 0.540852 2.4:+50179250 None:intergenic
TATTGGAGCAAGCTGAGAGA+AGG 0.543360 2.4:+50180711 MS.gene025132:CDS
TATTATAAATCTACTGAGGA+AGG 0.543578 2.4:+50180653 MS.gene025132:CDS
ATGAAGGCGTTGTCTAGAGC+AGG 0.543907 2.4:+50181124 MS.gene025132:CDS
AAGCAATTCTGAAATTTGAA+AGG 0.544332 2.4:-50179764 None:intergenic
ATATACAAACGCAAAGAACT+AGG 0.544709 2.4:+50180257 MS.gene025132:CDS
TAAATCTACTGAGGAAGGAA+AGG 0.549862 2.4:+50180658 MS.gene025132:CDS
GGCACCATTTAAAATAAAAG+AGG 0.551112 2.4:+50181087 MS.gene025132:CDS
TATTGTATCTTTGTTAACGA+AGG 0.551812 2.4:+50180553 MS.gene025132:CDS
CCTTCTGAACTCACTTTCGA+TGG 0.555532 2.4:+50180584 MS.gene025132:CDS
GGAGCATCTAATTCGGATGA+AGG 0.559886 2.4:+50181108 MS.gene025132:CDS
CATCTTGAGCAGTGGAGTTG+GGG 0.560586 2.4:+50181407 MS.gene025132:intron
TGCGAGCTGCGATAGGTCAT+CGG 0.561459 2.4:-50181479 None:intergenic
AGAGATGCCTCACCATGTAA+AGG 0.569360 2.4:-50180737 None:intergenic
CCATGCATGCGAGCTGCGAT+AGG 0.569820 2.4:-50181486 None:intergenic
GAGAGAAGGGAGCCTTTACA+TGG 0.569857 2.4:+50180725 MS.gene025132:CDS
CGACAAAATGCACCGGTGCA+CGG 0.570525 2.4:-50179488 None:intergenic
CCATCGAAAGTGAGTTCAGA+AGG 0.573318 2.4:-50180584 None:intergenic
TAAAGATGTTTGTGTTTGTG+TGG 0.576566 2.4:+50179669 MS.gene025132:CDS
TTTGTTTCCGATGGAAGCAA+AGG 0.576921 2.4:+50180976 MS.gene025132:CDS
CTTCAATACTAGATGATACA+GGG 0.581620 2.4:+50179413 MS.gene025132:CDS
GACAACTATGGACTTGAATG+AGG 0.586182 2.4:+50181066 MS.gene025132:CDS
CGAATTTGGGTGGTTGCCAG+AGG 0.588649 2.4:+50181044 MS.gene025132:CDS
GAATTTACTCCGAATTTGGG+TGG 0.588904 2.4:+50181034 MS.gene025132:CDS
GATGGAAGCAAAGGTTGTGA+TGG 0.592814 2.4:+50180985 MS.gene025132:CDS
CCACGTCAATAGGAATGCCA+AGG 0.596790 2.4:+50181638 MS.gene025132:CDS
AAGCTAGATTATCTGAGAGA+CGG 0.602323 2.4:-50179393 None:intergenic
TCCTCACCGACAAAATGCAC+CGG 0.603407 2.4:-50179495 None:intergenic
AACGAAATCAGCAACAACAC+TGG 0.604355 2.4:+50179289 MS.gene025132:CDS
CCTTGGCATTCCTATTGACG+TGG 0.613810 2.4:-50181638 None:intergenic
AAAGCGTAGTACGAAAGCTG+TGG 0.616351 2.4:+50180628 MS.gene025132:CDS
AAAATCAAGAAGCTCAGTAG+TGG 0.618055 2.4:-50180450 None:intergenic
AAGGGAGCCTTTACATGGTG+AGG 0.619314 2.4:+50180730 MS.gene025132:CDS
GGCAATAGATGACATGTTGG+TGG 0.623200 2.4:+50180100 MS.gene025132:CDS
GGTCTTCCATGAGGACAAAG+AGG 0.638424 2.4:+50181579 MS.gene025132:CDS
AGTGAAGCGCAGACGATACG+CGG 0.639553 2.4:+50181534 MS.gene025132:CDS
AATGGCAATAGATGACATGT+TGG 0.639924 2.4:+50180097 MS.gene025132:CDS
CACGTCAATAGGAATGCCAA+GGG 0.651995 2.4:+50181639 MS.gene025132:CDS
AGGACACACTACTCTAGATG+AGG 0.655016 2.4:+50180385 MS.gene025132:CDS
TTATCTGAGAGACGGTTCAG+AGG 0.662304 2.4:-50179385 None:intergenic
TCTTAATAAGGTCTTCCATG+AGG 0.672788 2.4:+50181570 MS.gene025132:CDS
TCAACACTTCCGAGCAACGA+GGG 0.684586 2.4:-50181441 None:intergenic
TTCAACACTTCCGAGCAACG+AGG 0.701823 2.4:-50181442 None:intergenic
GGATTATTATAAATCTACTG+AGG 0.714422 2.4:+50180649 MS.gene025132:CDS
TTGTTGTACCTAGAAAACCG+AGG 0.809311 2.4:+50180797 MS.gene025132:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATTAATTTAACTGAA+AGG - chr2.4:50179993-50180012 None:intergenic 10.0%
!! AAAGTTAAAGTAATTTCATA+GGG + chr2.4:50179848-50179867 MS.gene025132:intron 15.0%
!! ATCTAACTTATAATGAAAAT+TGG - chr2.4:50181322-50181341 None:intergenic 15.0%
!! ATGTAAATCATAAAAATCTT+AGG + chr2.4:50180483-50180502 MS.gene025132:CDS 15.0%
!! TATATTATTTGTTTCACTAT+AGG - chr2.4:50180242-50180261 None:intergenic 15.0%
!! TCAAAAATAAAATGAAAGAT+GGG - chr2.4:50181166-50181185 None:intergenic 15.0%
!! TCAACATTTATTATGAAATT+CGG - chr2.4:50180914-50180933 None:intergenic 15.0%
!! TGCAAAATTTAACAAATAAA+TGG - chr2.4:50181353-50181372 None:intergenic 15.0%
!! TGTAAATCATAAAAATCTTA+GGG + chr2.4:50180484-50180503 MS.gene025132:CDS 15.0%
!!! AACAAAGATACAATAATTTT+CGG - chr2.4:50180548-50180567 None:intergenic 15.0%
!!! CATTTTAGATTTATATACAA+TGG - chr2.4:50181231-50181250 None:intergenic 15.0%
!! AAGTTAAAGTAATTTCATAG+GGG + chr2.4:50179849-50179868 MS.gene025132:intron 20.0%
!! CAACATTTATTATGAAATTC+GGG - chr2.4:50180913-50180932 None:intergenic 20.0%
!! CTCAAAAATAAAATGAAAGA+TGG - chr2.4:50181167-50181186 None:intergenic 20.0%
!! GAAAGTTAAAGTAATTTCAT+AGG + chr2.4:50179847-50179866 MS.gene025132:intron 20.0%
!! GTAAAGAAAGAAAAATAGAA+TGG - chr2.4:50181257-50181276 None:intergenic 20.0%
!! GTAAATCATAAAAATCTTAG+GGG + chr2.4:50180485-50180504 MS.gene025132:CDS 20.0%
!!! ATTTTTGAGTACTTTCTTAA+AGG + chr2.4:50181178-50181197 MS.gene025132:intron 20.0%
!!! TTCTTTAAGGATGTTTTTAA+GGG + chr2.4:50179523-50179542 MS.gene025132:CDS 20.0%
!!! TTTCTTTAAGGATGTTTTTA+AGG + chr2.4:50179522-50179541 MS.gene025132:CDS 20.0%
! ATGTTTGTCTAGAAGATTTA+CGG - chr2.4:50180300-50180319 None:intergenic 25.0%
! ATTGTATCTTTGTTAACGAA+GGG + chr2.4:50180554-50180573 MS.gene025132:CDS 25.0%
! GAAGTAGTAATAGTTGAATT+TGG - chr2.4:50179340-50179359 None:intergenic 25.0%
! GGATTATTATAAATCTACTG+AGG + chr2.4:50180649-50180668 MS.gene025132:CDS 25.0%
! GTTCGAAAACAATTGATATA+GGG + chr2.4:50181295-50181314 MS.gene025132:intron 25.0%
! TATTATAAATCTACTGAGGA+AGG + chr2.4:50180653-50180672 MS.gene025132:CDS 25.0%
! TATTGTATCTTTGTTAACGA+AGG + chr2.4:50180553-50180572 MS.gene025132:CDS 25.0%
! TTTCTTTACTTGTATCTATC+TGG + chr2.4:50181268-50181287 MS.gene025132:intron 25.0%
!!! AAGCAATTCTGAAATTTGAA+AGG - chr2.4:50179767-50179786 None:intergenic 25.0%
!!! TGCTCCTCTTTTATTTTAAA+TGG - chr2.4:50181094-50181113 None:intergenic 25.0%
AAAGAGGAGTTTGATAAATC+TGG + chr2.4:50181595-50181614 MS.gene025132:CDS 30.0%
ACTAGACATTCTTGACAAAA+TGG + chr2.4:50180079-50180098 MS.gene025132:CDS 30.0%
ATATACAAACGCAAAGAACT+AGG + chr2.4:50180257-50180276 MS.gene025132:CDS 30.0%
ATCATACTATTATTAGCCCT+TGG - chr2.4:50181658-50181677 None:intergenic 30.0%
ATCTGAATTTACTCCGAATT+TGG + chr2.4:50181030-50181049 MS.gene025132:CDS 30.0%
ATGGATATCAATCCAATTGA+TGG + chr2.4:50181004-50181023 MS.gene025132:CDS 30.0%
ATTGTTTACACTTTGGTTGT+TGG + chr2.4:50179698-50179717 MS.gene025132:CDS 30.0%
CAGATGATGTAGAATTAGTA+AGG + chr2.4:50180348-50180367 MS.gene025132:CDS 30.0%
CTTCAATACTAGATGATACA+GGG + chr2.4:50179413-50179432 MS.gene025132:CDS 30.0%
CTTTCTATAGCAAACATTTG+TGG + chr2.4:50180125-50180144 MS.gene025132:CDS 30.0%
GAACATGTAAGAAAATACCT+CGG - chr2.4:50180817-50180836 None:intergenic 30.0%
GATGATGATTGTTTACACTT+TGG + chr2.4:50179691-50179710 MS.gene025132:CDS 30.0%
GCTACAAGAAGTTCTTAATA+AGG + chr2.4:50181558-50181577 MS.gene025132:CDS 30.0%
GGTTCGAAAACAATTGATAT+AGG + chr2.4:50181294-50181313 MS.gene025132:intron 30.0%
TAAAATGAAAGATGGGAAAC+AGG - chr2.4:50181159-50181178 None:intergenic 30.0%
TAAAGATGTTTGTGTTTGTG+TGG + chr2.4:50179669-50179688 MS.gene025132:CDS 30.0%
TCTGAATTTACTCCGAATTT+GGG + chr2.4:50181031-50181050 MS.gene025132:CDS 30.0%
TGACTAAGGATTATGATGTT+GGG + chr2.4:50179578-50179597 MS.gene025132:CDS 30.0%
TTACTTGTATCTATCTGGTT+CGG + chr2.4:50181273-50181292 MS.gene025132:intron 30.0%
! GGCACCATTTAAAATAAAAG+AGG + chr2.4:50181087-50181106 MS.gene025132:CDS 30.0%
!! ACGATTCATTGTTTTTCAGT+TGG + chr2.4:50180941-50180960 MS.gene025132:intron 30.0%
!! TAAAAGAGGAGCATCTAATT+CGG + chr2.4:50181101-50181120 MS.gene025132:CDS 30.0%
AAAATCAAGAAGCTCAGTAG+TGG - chr2.4:50180453-50180472 None:intergenic 35.0%
AAGCTAGATTATCTGAGAGA+CGG - chr2.4:50179396-50179415 None:intergenic 35.0%
AATGGCAATAGATGACATGT+TGG + chr2.4:50180097-50180116 MS.gene025132:CDS 35.0%
AATTCAGATGTGCCATCAAT+TGG - chr2.4:50181019-50181038 None:intergenic 35.0%
AGAATTGCTTGCTCTTTATC+AGG + chr2.4:50179777-50179796 MS.gene025132:CDS 35.0%
AGCCTCTTGTGTAAAAAACA+GGG - chr2.4:50179948-50179967 None:intergenic 35.0%
AGTACTTTCTTAAAGGTCGA+AGG + chr2.4:50181185-50181204 MS.gene025132:intron 35.0%
GCTTCAATACTAGATGATAC+AGG + chr2.4:50179412-50179431 MS.gene025132:CDS 35.0%
GTGACTAAGGATTATGATGT+TGG + chr2.4:50179577-50179596 MS.gene025132:CDS 35.0%
GTTCTGCGTTATTTGTATAG+TGG + chr2.4:50179619-50179638 MS.gene025132:CDS 35.0%
TAAATCTACTGAGGAAGGAA+AGG + chr2.4:50180658-50180677 MS.gene025132:CDS 35.0%
TCGATTGTGTATCGAGATAT+TGG + chr2.4:50180694-50180713 MS.gene025132:CDS 35.0%
TCTTAATAAGGTCTTCCATG+AGG + chr2.4:50181570-50181589 MS.gene025132:CDS 35.0%
TGAAGCATGAAATCAACAAC+AGG - chr2.4:50179724-50179743 None:intergenic 35.0%
! ACTACTGAGCTTCTTGATTT+TGG + chr2.4:50180452-50180471 MS.gene025132:CDS 35.0%
! AGAAAATACCTCGGTTTTCT+AGG - chr2.4:50180808-50180827 None:intergenic 35.0%
! ATCTAAAGCTCTGTGTATTC+TGG - chr2.4:50180327-50180346 None:intergenic 35.0%
!! AGCAAAACTTTTGTTTCCGA+TGG + chr2.4:50180967-50180986 MS.gene025132:CDS 35.0%
!! CAGTTGATTTGATTACTTGC+AGG + chr2.4:50180049-50180068 MS.gene025132:intron 35.0%
!!! AGTCAGTGATTTTTCAAGAG+TGG - chr2.4:50180216-50180235 None:intergenic 35.0%
!!! GTAAGGTTGCTTTTGAAAGA+AGG + chr2.4:50180365-50180384 MS.gene025132:CDS 35.0%
!!! TACCCTGTTTTTTACACAAG+AGG + chr2.4:50179943-50179962 MS.gene025132:intron 35.0%
AACGAAATCAGCAACAACAC+TGG + chr2.4:50179289-50179308 MS.gene025132:CDS 40.0%
AAGGTCACAAGTTCAAGTCT+CGG - chr2.4:50179974-50179993 None:intergenic 40.0%
ACGCGTACAATACAGCAAAA+TGG - chr2.4:50179920-50179939 None:intergenic 40.0%
ATCACAACCTTTGCTTCCAT+CGG - chr2.4:50180986-50181005 None:intergenic 40.0%
ATTCGGGAGTAACATATGTC+AGG - chr2.4:50180897-50180916 None:intergenic 40.0%
CAGCCTCTTGTGTAAAAAAC+AGG - chr2.4:50179949-50179968 None:intergenic 40.0%
GAATTTACTCCGAATTTGGG+TGG + chr2.4:50181034-50181053 MS.gene025132:CDS 40.0%
GACAACTATGGACTTGAATG+AGG + chr2.4:50181066-50181085 MS.gene025132:CDS 40.0%
GCAACTAAAGGAAGTGACTA+AGG + chr2.4:50179564-50179583 MS.gene025132:CDS 40.0%
GGTGAGGAGTAGTTTCTTTA+AGG + chr2.4:50179510-50179529 MS.gene025132:CDS 40.0%
TCTGGCGATGATCTTTGAAT+CGG - chr2.4:50179452-50179471 None:intergenic 40.0%
TTCGGGAGTAACATATGTCA+GGG - chr2.4:50180896-50180915 None:intergenic 40.0%
TTGTTGTACCTAGAAAACCG+AGG + chr2.4:50180797-50180816 MS.gene025132:CDS 40.0%
TTTGTTTCCGATGGAAGCAA+AGG + chr2.4:50180976-50180995 MS.gene025132:CDS 40.0%
!! GGAAGTGTTGAACAAGATCA+TGG + chr2.4:50181453-50181472 MS.gene025132:CDS 40.0%
!! TTTTTGGTCATCTTGAGCAG+TGG + chr2.4:50181399-50181418 MS.gene025132:intron 40.0%
AAAATGGTAAGACCCCTTGC+AGG - chr2.4:50179904-50179923 None:intergenic 45.0%
AAAGCGTAGTACGAAAGCTG+TGG + chr2.4:50180628-50180647 MS.gene025132:CDS 45.0%
AGAAACGACGCTGCAACTAA+AGG + chr2.4:50179552-50179571 MS.gene025132:CDS 45.0%
AGAGATGCCTCACCATGTAA+AGG - chr2.4:50180740-50180759 None:intergenic 45.0%
AGGACACACTACTCTAGATG+AGG + chr2.4:50180385-50180404 MS.gene025132:CDS 45.0%
ATTGGAGCAAGCTGAGAGAA+GGG + chr2.4:50180712-50180731 MS.gene025132:CDS 45.0%
CAAACTCCTCTTTGTCCTCA+TGG - chr2.4:50181588-50181607 None:intergenic 45.0%
CACGTCAATAGGAATGCCAA+GGG + chr2.4:50181639-50181658 MS.gene025132:CDS 45.0%
CATTCCTATTGACGTGGAAG+AGG - chr2.4:50181635-50181654 None:intergenic 45.0%
CCATCGAAAGTGAGTTCAGA+AGG - chr2.4:50180587-50180606 None:intergenic 45.0%
CCTTCTGAACTCACTTTCGA+TGG + chr2.4:50180584-50180603 MS.gene025132:CDS 45.0%
CTCTTTCTCTTGCTAAGGCA+GGG + chr2.4:50180756-50180775 MS.gene025132:CDS 45.0%
GATGGAAGCAAAGGTTGTGA+TGG + chr2.4:50180985-50181004 MS.gene025132:CDS 45.0%
GGCAATAGATGACATGTTGG+TGG + chr2.4:50180100-50180119 MS.gene025132:CDS 45.0%
GGCATCTCTTTCTCTTGCTA+AGG + chr2.4:50180751-50180770 MS.gene025132:CDS 45.0%
TATTGGAGCAAGCTGAGAGA+AGG + chr2.4:50180711-50180730 MS.gene025132:CDS 45.0%
TCAAAGATCATCGCCAGAGA+TGG + chr2.4:50179454-50179473 MS.gene025132:CDS 45.0%
TCAAGTCCATAGTTGTCCTC+TGG - chr2.4:50181063-50181082 None:intergenic 45.0%
TCATCCTCTTCCACGTCAAT+AGG + chr2.4:50181628-50181647 MS.gene025132:CDS 45.0%
TCATCTTGAGCAGTGGAGTT+GGG + chr2.4:50181406-50181425 MS.gene025132:intron 45.0%
TCGTGCACTAAAACTAGGCA+GGG + chr2.4:50179877-50179896 MS.gene025132:intron 45.0%
TCTCTTTCTCTTGCTAAGGC+AGG + chr2.4:50180755-50180774 MS.gene025132:CDS 45.0%
TCTTTCTCTTGCTAAGGCAG+GGG + chr2.4:50180757-50180776 MS.gene025132:CDS 45.0%
TTATCTGAGAGACGGTTCAG+AGG - chr2.4:50179388-50179407 None:intergenic 45.0%
! GGAGCATCTAATTCGGATGA+AGG + chr2.4:50181108-50181127 MS.gene025132:CDS 45.0%
!!! TATATTTTATTTTGATTTTT+TGG + chr2.4:50181383-50181402 MS.gene025132:intron 5.0%
AAGGGAGCCTTTACATGGTG+AGG + chr2.4:50180730-50180749 MS.gene025132:CDS 50.0%
CAGCCTCGTGCACTAAAACT+AGG + chr2.4:50179872-50179891 MS.gene025132:intron 50.0%
CATCTTGAGCAGTGGAGTTG+GGG + chr2.4:50181407-50181426 MS.gene025132:intron 50.0%
CCACGTCAATAGGAATGCCA+AGG + chr2.4:50181638-50181657 MS.gene025132:CDS 50.0%
CCTTGGCATTCCTATTGACG+TGG - chr2.4:50181641-50181660 None:intergenic 50.0%
CGCTTCACTTGTCGATCTTC+GGG - chr2.4:50181523-50181542 None:intergenic 50.0%
CTCGTGCACTAAAACTAGGC+AGG + chr2.4:50179876-50179895 MS.gene025132:intron 50.0%
CTCTGGCAACCACCCAAATT+CGG - chr2.4:50181046-50181065 None:intergenic 50.0%
GAGAGAAGGGAGCCTTTACA+TGG + chr2.4:50180725-50180744 MS.gene025132:CDS 50.0%
GCGCTTCACTTGTCGATCTT+CGG - chr2.4:50181524-50181543 None:intergenic 50.0%
GGAAACTTCTCGACCATCTC+TGG - chr2.4:50179470-50179489 None:intergenic 50.0%
GGTCTTCCATGAGGACAAAG+AGG + chr2.4:50181579-50181598 MS.gene025132:CDS 50.0%
GTCATCTTGAGCAGTGGAGT+TGG + chr2.4:50181405-50181424 MS.gene025132:intron 50.0%
TCAACACTTCCGAGCAACGA+GGG - chr2.4:50181444-50181463 None:intergenic 50.0%
TCCTCACCGACAAAATGCAC+CGG - chr2.4:50179498-50179517 None:intergenic 50.0%
TGGTTGCCAGAGGACAACTA+TGG + chr2.4:50181054-50181073 MS.gene025132:CDS 50.0%
TTCAACACTTCCGAGCAACG+AGG - chr2.4:50181445-50181464 None:intergenic 50.0%
!! ATGAAGGCGTTGTCTAGAGC+AGG + chr2.4:50181124-50181143 MS.gene025132:CDS 50.0%
!!! CTGCCTAGTTTTAGTGCACG+AGG - chr2.4:50179878-50179897 None:intergenic 50.0%
AAACTAGGCAGGGTCCTGCA+AGG + chr2.4:50179887-50179906 MS.gene025132:intron 55.0%
AACTAGGCAGGGTCCTGCAA+GGG + chr2.4:50179888-50179907 MS.gene025132:intron 55.0%
ACGGTTCTTCCCTCGTTGCT+CGG + chr2.4:50181432-50181451 MS.gene025132:CDS 55.0%
AGTGAAGCGCAGACGATACG+CGG + chr2.4:50181534-50181553 MS.gene025132:CDS 55.0%
CGAATTTGGGTGGTTGCCAG+AGG + chr2.4:50181044-50181063 MS.gene025132:CDS 55.0%
CTATCGCAGCTCGCATGCAT+GGG + chr2.4:50181487-50181506 MS.gene025132:CDS 55.0%
GAGCAGTGGAGTTGGGGAAA+CGG + chr2.4:50181413-50181432 MS.gene025132:intron 55.0%
GTCGAGAAGTTTCCGTGCAC+CGG + chr2.4:50179476-50179495 MS.gene025132:CDS 55.0%
TGCGAGCTGCGATAGGTCAT+CGG - chr2.4:50181482-50181501 None:intergenic 55.0%
! ACCGGTGCATTTTGTCGGTG+AGG + chr2.4:50179494-50179513 MS.gene025132:CDS 55.0%
! CGACAAAATGCACCGGTGCA+CGG - chr2.4:50179491-50179510 None:intergenic 55.0%
!! CGTGCACCGGTGCATTTTGT+CGG + chr2.4:50179489-50179508 MS.gene025132:CDS 55.0%
ACTAGGCAGGGTCCTGCAAG+GGG + chr2.4:50179889-50179908 MS.gene025132:intron 60.0%
CCATGCATGCGAGCTGCGAT+AGG - chr2.4:50181489-50181508 None:intergenic 60.0%
CCTATCGCAGCTCGCATGCA+TGG + chr2.4:50181486-50181505 MS.gene025132:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 50179253 50181693 50179253 ID=MS.gene025132
chr2.4 mRNA 50179253 50181693 50179253 ID=MS.gene025132.t1;Parent=MS.gene025132
chr2.4 exon 50179253 50179798 50179253 ID=MS.gene025132.t1.exon1;Parent=MS.gene025132.t1
chr2.4 CDS 50179253 50179798 50179253 ID=cds.MS.gene025132.t1;Parent=MS.gene025132.t1
chr2.4 exon 50180071 50180818 50180071 ID=MS.gene025132.t1.exon2;Parent=MS.gene025132.t1
chr2.4 CDS 50180071 50180818 50180071 ID=cds.MS.gene025132.t1;Parent=MS.gene025132.t1
chr2.4 exon 50180960 50181145 50180960 ID=MS.gene025132.t1.exon3;Parent=MS.gene025132.t1
chr2.4 CDS 50180960 50181145 50180960 ID=cds.MS.gene025132.t1;Parent=MS.gene025132.t1
chr2.4 exon 50181419 50181693 50181419 ID=MS.gene025132.t1.exon4;Parent=MS.gene025132.t1
chr2.4 CDS 50181419 50181693 50181419 ID=cds.MS.gene025132.t1;Parent=MS.gene025132.t1
Gene Sequence

>MS.gene025132

ATGATGTACTTGAGAAGTGGATTCTCTGAATATTCAAACGAAATCAGCAACAACACTGGCAGTGAGTTATGCTGCACAACAGCACCAAATTCAACTATTACTACTTCACAAGATATCATCACAATCACACAACCTCTGAACCGTCTCTCAGATAATCTAGCTTCAATACTAGATGATACAGGGTTTGACTTCTTTTCCGATTCAAAGATCATCGCCAGAGATGGTCGAGAAGTTTCCGTGCACCGGTGCATTTTGTCGGTGAGGAGTAGTTTCTTTAAGGATGTTTTTAAGGGAAAGAAAGAAACGACGCTGCAACTAAAGGAAGTGACTAAGGATTATGATGTTGGGTTTGACGCGCTTAACGTTGTTCTGCGTTATTTGTATAGTGGCAGAGTTGATCATCATCATTTGTCTGCTAAAGATGTTTGTGTTTGTGTGGATGATGATTGTTTACACTTTGGTTGTTGGCCTGTTGTTGATTTCATGCTTCAACTTCTCTATGCTTCATTCACCTTTCAAATTTCAGAATTGCTTGCTCTTTATCAGGTAAGAATTAAGCACTTATAGATGCTATTTTAATAATAAAAGATGAAAGAAAGTTAAAGTAATTTCATAGGGGCAGCCTCGTGCACTAAAACTAGGCAGGGTCCTGCAAGGGGTCTTACCATTTTGCTGTATTGTACGCGTTCTTACCCTGTTTTTTACACAAGAGGCTGTTCCGAGACTTGAACTTGTGACCTTTCAGTTAAATTAATTTTATATAATTTATTAAAATAATCTTAAAACAATTTCTTAACAGTTGATTTGATTACTTGCAGGATCATCTACTAGACATTCTTGACAAAATGGCAATAGATGACATGTTGGTGGTTCTTTCTATAGCAAACATTTGTGGTAAAACATGTGATAAACTACTAACAAGATGTACAGAGATAATAGTTGAATCTAATGTTGATATAACCACTCTTGAAAAATCACTGACTCAACCTATAGTGAAACAAATAATATACAAACGCAAAGAACTAGGTTTAGATATGTATGAAACCGTAAATCTTCTAGACAAACATGTCACCAGAATACACAGAGCTTTAGATTCAGATGATGTAGAATTAGTAAGGTTGCTTTTGAAAGAAGGACACACTACTCTAGATGAGGCTCATGCACTTCACTATGCTGTTGCATATTGTGATGTCAAAACCACTACTGAGCTTCTTGATTTTGGACTTGCTGATGTAAATCATAAAAATCTTAGGGGCTATAGCGTGCTACATGTTGCTGCGAAGAGAAAAGAACCGAAAATTATTGTATCTTTGTTAACGAAGGGAGCTCAACCTTCTGAACTCACTTTCGATGGCAGAAAAGCTCTTCAAATTTCAAAGCGTAGTACGAAAGCTGTGGATTATTATAAATCTACTGAGGAAGGAAAGGTTTCTTCGAATGATCGATTGTGTATCGAGATATTGGAGCAAGCTGAGAGAAGGGAGCCTTTACATGGTGAGGCATCTCTTTCTCTTGCTAAGGCAGGGGATGATTTACGTATGAAATTGTTGTACCTAGAAAACCGAGGTATTTTCTTACATGTTCATATTTTTCGATTTATTTTCTTTTTTTCGATAAATGTTGAATCAACATTTGTGTCGCCCTGACATATGTTACTCCCGAATTTCATAATAAATGTTGATCAACTGACGATTCATTGTTTTTCAGTTGGACTAGCAAAACTTTTGTTTCCGATGGAAGCAAAGGTTGTGATGGATATCAATCCAATTGATGGCACATCTGAATTTACTCCGAATTTGGGTGGTTGCCAGAGGACAACTATGGACTTGAATGAGGCACCATTTAAAATAAAAGAGGAGCATCTAATTCGGATGAAGGCGTTGTCTAGAGCAGGTAATATATACCTGTTTCCCATCTTTCATTTTATTTTTGAGTACTTTCTTAAAGGTCGAAGGCTCAAAGTAATTCTTATCAACCATTGTATATAAATCTAAAATGTTTCCATTCTATTTTTCTTTCTTTACTTGTATCTATCTGGTTCGGTTCGAAAACAATTGATATAGGGACCAATTTTCATTATAAGTTAGATTTTAGTCACCATTTATTTGTTAAATTTTGCAAATTGATGCTTATATTTTATTTTGATTTTTTGGTCATCTTGAGCAGTGGAGTTGGGGAAACGGTTCTTCCCTCGTTGCTCGGAAGTGTTGAACAAGATCATGGATGCCGATGACCTATCGCAGCTCGCATGCATGGGTCATGACAGTCCCGAAGATCGACAAGTGAAGCGCAGACGATACGCGGAGCTACAAGAAGTTCTTAATAAGGTCTTCCATGAGGACAAAGAGGAGTTTGATAAATCTGGCATGTCTTCATCATCCTCTTCCACGTCAATAGGAATGCCAAGGGCTAATAATAGTATGATAGCAATGAAACATTGA
Protein sequence

>MS.gene025132.t1

MMYLRSGFSEYSNEISNNTGSELCCTTAPNSTITTSQDIITITQPLNRLSDNLASILDDTGFDFFSDSKIIARDGREVSVHRCILSVRSSFFKDVFKGKKETTLQLKEVTKDYDVGFDALNVVLRYLYSGRVDHHHLSAKDVCVCVDDDCLHFGCWPVVDFMLQLLYASFTFQISELLALYQDHLLDILDKMAIDDMLVVLSIANICGKTCDKLLTRCTEIIVESNVDITTLEKSLTQPIVKQIIYKRKELGLDMYETVNLLDKHVTRIHRALDSDDVELVRLLLKEGHTTLDEAHALHYAVAYCDVKTTTELLDFGLADVNHKNLRGYSVLHVAAKRKEPKIIVSLLTKGAQPSELTFDGRKALQISKRSTKAVDYYKSTEEGKVSSNDRLCIEILEQAERREPLHGEASLSLAKAGDDLRMKLLYLENRVGLAKLLFPMEAKVVMDINPIDGTSEFTPNLGGCQRTTMDLNEAPFKIKEEHLIRMKALSRAVELGKRFFPRCSEVLNKIMDADDLSQLACMGHDSPEDRQVKRRRYAELQEVLNKVFHEDKEEFDKSGMSSSSSSTSIGMPRANNSMIAMKH