AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025402

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025402.t1 MTR_2g045360 95.972 422 16 1 1 421 8 429 0.0 797
MS.gene025402.t1 MTR_3g053330 48.261 230 104 7 178 402 225 444 5.71e-58 197
MS.gene025402.t1 MTR_7g110720 35.262 363 181 12 57 408 92 411 2.66e-53 184
MS.gene025402.t1 MTR_7g110720 41.111 270 130 9 150 408 133 384 1.46e-52 181
MS.gene025402.t1 MTR_4g132430 37.410 278 159 4 61 331 114 383 2.22e-49 174
MS.gene025402.t1 MTR_2g088000 35.872 407 178 15 59 401 167 554 7.92e-49 175
MS.gene025402.t1 MTR_4g132430 38.521 257 143 4 82 331 1 249 1.49e-47 166
MS.gene025402.t1 MTR_2g023930 33.002 403 197 11 61 404 143 531 3.87e-45 164
MS.gene025402.t1 MTR_4g049790 35.241 332 164 7 61 350 131 453 3.23e-44 162
MS.gene025402.t1 MTR_4g049760 55.696 158 63 3 175 331 261 412 3.30e-41 153
MS.gene025402.t1 MTR_4g094412 50.331 151 66 3 188 331 218 366 4.88e-37 141
MS.gene025402.t1 MTR_4g094412 50.331 151 66 3 188 331 227 375 5.21e-37 141
MS.gene025402.t1 MTR_4g107970 63.158 95 31 1 174 264 144 238 8.28e-37 141
MS.gene025402.t1 MTR_4g107970 63.158 95 31 1 174 264 221 315 1.82e-36 141
MS.gene025402.t1 MTR_7g009730 61.386 101 38 1 178 277 192 292 4.94e-36 139
MS.gene025402.t1 MTR_4g047580 38.314 261 134 8 159 401 239 490 1.27e-34 135
MS.gene025402.t1 MTR_4g007060 32.110 218 97 8 185 399 107 276 3.76e-24 101
MS.gene025402.t1 MTR_4g007060 42.857 105 58 2 185 287 141 245 6.38e-24 101
MS.gene025402.t1 MTR_5g074400 49.020 102 50 2 165 266 382 481 1.59e-23 103
MS.gene025402.t1 MTR_2g105060 40.458 131 68 3 187 312 72 197 1.40e-22 96.3
MS.gene025402.t1 MTR_1g013790 48.235 85 42 2 202 284 99 183 2.98e-22 95.5
MS.gene025402.t1 MTR_2g105060 45.714 105 52 2 187 286 147 251 3.10e-22 97.1
MS.gene025402.t1 MTR_3g106060 50.602 83 38 1 202 284 136 215 3.30e-22 96.3
MS.gene025402.t1 MTR_7g109800 37.671 146 80 4 188 323 129 273 4.37e-22 96.3
MS.gene025402.t1 MTR_1g013760 50.685 73 36 0 189 261 125 197 5.68e-22 95.5
MS.gene025402.t1 MTR_5g094430 45.556 90 48 1 188 277 236 324 2.21e-21 95.9
MS.gene025402.t1 MTR_5g094430 45.556 90 48 1 188 277 214 302 2.27e-21 95.9
MS.gene025402.t1 MTR_3g009470 37.584 149 81 5 154 292 435 581 2.86e-21 96.7
MS.gene025402.t1 MTR_3g009470 39.362 94 54 3 176 268 251 342 2.40e-13 72.4
MS.gene025402.t1 MTR_5g094430 50.633 79 38 1 188 266 348 425 3.60e-21 96.3
MS.gene025402.t1 MTR_7g079010 35.088 171 85 6 109 274 58 207 8.04e-21 90.9
MS.gene025402.t1 MTR_7g100510 44.954 109 52 3 177 280 329 434 8.43e-21 94.4
MS.gene025402.t1 MTR_7g100510 42.308 78 43 2 205 282 190 265 3.49e-12 68.2
MS.gene025402.t1 MTR_3g031220 51.282 78 37 1 187 264 348 424 1.01e-20 94.7
MS.gene025402.t1 MTR_3g056100 50.633 79 38 1 188 266 255 332 1.28e-20 94.0
MS.gene025402.t1 MTR_3g056100 50.633 79 38 1 188 266 242 319 1.28e-20 94.0
MS.gene025402.t1 MTR_3g056100 50.633 79 38 1 188 266 399 476 1.51e-20 94.4
MS.gene025402.t1 MTR_3g056100 50.633 79 38 1 188 266 386 463 1.61e-20 94.4
MS.gene025402.t1 MTR_5g044695 65.517 58 19 1 176 232 48 105 1.66e-20 86.7
MS.gene025402.t1 MTR_4g013260 50.000 82 37 2 186 264 315 395 2.88e-20 93.2
MS.gene025402.t1 MTR_4g013260 42.647 68 38 1 205 272 185 251 9.53e-12 67.0
MS.gene025402.t1 MTR_7g011820 47.674 86 44 1 191 276 397 481 5.85e-20 92.4
MS.gene025402.t1 MTR_7g011820 48.333 60 29 2 205 264 229 286 1.23e-11 66.6
MS.gene025402.t1 MTR_7g080020 48.148 81 41 1 186 266 475 554 7.65e-20 92.4
MS.gene025402.t1 MTR_7g080020 45.312 64 33 2 205 268 281 342 2.68e-11 65.9
MS.gene025402.t1 MTR_4g082580 45.977 87 46 1 189 275 263 348 7.92e-20 90.9
MS.gene025402.t1 MTR_7g080000 36.641 131 70 2 186 304 414 543 9.78e-20 92.0
MS.gene025402.t1 MTR_7g080000 48.387 62 30 2 203 264 233 292 2.21e-11 66.2
MS.gene025402.t1 MTR_4g122530 47.674 86 43 2 188 273 192 275 1.58e-19 90.5
MS.gene025402.t1 MTR_5g042920 45.161 93 47 2 191 280 307 398 1.67e-19 90.5
MS.gene025402.t1 MTR_5g042920 50.000 60 28 2 205 264 152 209 5.73e-12 67.4
MS.gene025402.t1 MTR_6g038890 65.517 58 19 1 176 232 159 216 2.87e-19 86.3
MS.gene025402.t1 MTR_3g095040 51.351 74 35 1 191 264 105 177 3.28e-19 85.9
MS.gene025402.t1 MTR_2g024350 49.296 71 36 0 205 275 9 79 4.31e-19 86.3
MS.gene025402.t1 MTR_2g033820 45.122 82 44 1 188 269 187 267 5.46e-19 88.6
MS.gene025402.t1 MTR_5g097490 40.708 113 61 3 175 283 223 333 6.82e-19 88.2
MS.gene025402.t1 MTR_7g028415 45.263 95 47 2 171 261 56 149 1.17e-18 83.6
MS.gene025402.t1 MTR_7g028710 45.263 95 47 2 171 261 56 149 1.17e-18 83.6
MS.gene025402.t1 MTR_5g091390 34.711 121 75 2 151 268 101 220 1.18e-18 86.7
MS.gene025402.t1 MTR_1g086790 44.444 81 44 1 188 268 142 221 1.25e-18 86.7
MS.gene025402.t1 MTR_7g071120 47.500 80 41 1 187 266 307 385 2.02e-18 87.4
MS.gene025402.t1 MTR_2g016770 55.738 61 27 0 205 265 36 96 3.08e-18 83.6
MS.gene025402.t1 MTR_4g094588 44.872 78 42 1 191 268 165 241 3.28e-18 85.5
MS.gene025402.t1 MTR_2g024380 35.433 127 76 1 155 275 196 322 4.15e-18 86.7
MS.gene025402.t1 MTR_2g016770 55.738 61 27 0 205 265 28 88 4.19e-18 82.8
MS.gene025402.t1 MTR_8g092010 47.297 74 38 1 191 264 95 167 5.12e-18 82.0
MS.gene025402.t1 MTR_1g015140 49.315 73 36 1 189 261 87 158 7.76e-18 80.9
MS.gene025402.t1 MTR_4g094908 54.839 62 28 0 205 266 267 328 7.81e-18 84.7
MS.gene025402.t1 MTR_8g087000 56.667 60 26 0 205 264 78 137 2.21e-17 81.6
MS.gene025402.t1 MTR_8g087000 56.667 60 26 0 205 264 77 136 2.52e-17 81.3
MS.gene025402.t1 MTR_3g104750 39.560 91 54 1 171 261 71 160 2.56e-17 79.3
MS.gene025402.t1 MTR_5g016610 41.837 98 48 1 175 263 161 258 3.36e-17 81.6
MS.gene025402.t1 MTR_8g096730 36.893 103 61 2 174 273 37 138 3.68e-17 78.6
MS.gene025402.t1 MTR_5g043880 42.683 82 46 1 181 262 101 181 7.04e-17 80.9
MS.gene025402.t1 MTR_4g130900 44.156 77 42 1 188 264 343 418 1.00e-16 82.4
MS.gene025402.t1 MTR_1g077780 44.156 77 42 1 188 264 46 121 1.04e-16 77.0
MS.gene025402.t1 MTR_1g070455 52.459 61 29 0 205 265 294 354 1.18e-16 81.3
MS.gene025402.t1 MTR_7g109600 50.725 69 34 0 205 273 54 122 1.81e-16 79.3
MS.gene025402.t1 MTR_1g009613 52.542 59 28 0 205 263 263 321 4.63e-16 79.3
MS.gene025402.t1 MTR_2g083870 55.000 60 27 0 205 264 81 140 6.43e-16 77.8
MS.gene025402.t1 MTR_1g099600 43.678 87 49 0 204 290 185 271 7.83e-16 78.2
MS.gene025402.t1 MTR_8g007270 45.882 85 41 2 181 261 127 210 8.79e-16 76.6
MS.gene025402.t1 MTR_3g095080 40.625 96 54 2 187 282 127 219 1.33e-15 75.9
MS.gene025402.t1 MTR_2g032030 39.394 99 59 1 179 276 241 339 1.57e-15 78.6
MS.gene025402.t1 MTR_3g095080 40.625 96 54 2 187 282 151 243 1.60e-15 76.3
MS.gene025402.t1 MTR_2g032030 39.394 99 59 1 179 276 241 339 1.89e-15 78.2
MS.gene025402.t1 MTR_8g028565 51.429 70 34 0 205 274 156 225 2.27e-15 77.4
MS.gene025402.t1 MTR_8g098945 42.353 85 47 1 203 287 128 210 8.82e-15 74.7
MS.gene025402.t1 MTR_7g079040 50.000 70 35 0 205 274 155 224 1.24e-14 75.1
MS.gene025402.t1 MTR_0117s0040 49.153 59 30 0 204 262 74 132 1.60e-14 73.2
MS.gene025402.t1 MTR_4g127040 52.632 57 27 0 205 261 246 302 1.91e-14 74.3
MS.gene025402.t1 MTR_8g092140 48.438 64 32 1 198 261 154 216 6.91e-14 71.2
MS.gene025402.t1 MTR_3g108960 39.744 78 47 0 188 265 298 375 1.04e-13 72.8
MS.gene025402.t1 MTR_1g078260 28.272 191 127 5 72 261 123 304 1.24e-13 72.8
MS.gene025402.t1 MTR_3g009470 39.362 94 54 3 176 268 251 342 2.38e-13 72.0
MS.gene025402.t1 MTR_6g086555 52.308 65 29 2 205 268 207 270 5.20e-13 70.5
MS.gene025402.t1 MTR_4g007060 44.444 63 35 0 179 241 137 199 5.76e-13 68.6
MS.gene025402.t1 MTR_7g062220 52.632 57 26 1 205 261 125 180 1.14e-12 68.9
MS.gene025402.t1 MTR_8g069935 41.667 60 35 0 205 264 332 391 3.41e-12 68.2
MS.gene025402.t1 MTR_8g067650 45.000 60 33 0 205 264 340 399 5.81e-12 67.8
MS.gene025402.t1 MTR_6g053200 41.667 60 35 0 205 264 331 390 8.43e-12 67.4
MS.gene025402.t1 MTR_8g067635 45.000 60 33 0 205 264 350 409 8.66e-12 67.4
MS.gene025402.t1 MTR_3g031220 45.588 68 30 3 205 272 199 259 1.38e-11 66.2
MS.gene025402.t1 MTR_3g090860 40.230 87 44 2 205 289 134 214 3.13e-11 64.7
MS.gene025402.t1 MTR_5g074200 40.909 88 47 4 205 290 221 305 3.50e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025402.t1 AT4G04450 38.585 311 141 7 63 331 20 322 1.30e-54 187
MS.gene025402.t1 AT4G04450 38.585 311 141 7 63 331 114 416 1.44e-53 187
MS.gene025402.t1 AT1G62300 37.065 402 170 17 67 404 158 540 4.41e-51 181
MS.gene025402.t1 AT4G01720 34.865 370 190 9 59 399 96 443 9.04e-48 170
MS.gene025402.t1 AT4G22070 37.152 323 149 9 59 334 108 423 1.05e-44 163
MS.gene025402.t1 AT4G22070 37.152 323 149 9 59 334 108 423 1.05e-44 163
MS.gene025402.t1 AT4G22070 37.152 323 149 9 59 334 108 423 1.05e-44 163
MS.gene025402.t1 AT4G22070 37.152 323 149 9 59 334 108 423 1.05e-44 163
MS.gene025402.t1 AT5G15130 35.030 334 142 10 61 333 35 354 3.20e-42 156
MS.gene025402.t1 AT5G15130 51.534 163 63 4 180 333 157 312 8.83e-42 154
MS.gene025402.t1 AT1G68150 56.818 132 56 1 182 313 213 343 6.85e-41 149
MS.gene025402.t1 AT1G18860 32.951 349 152 9 61 340 41 376 9.10e-37 141
MS.gene025402.t1 AT1G69810 44.785 163 77 3 173 333 114 265 1.35e-33 128
MS.gene025402.t1 AT1G69810 45.570 158 73 3 178 333 177 323 1.63e-33 129
MS.gene025402.t1 AT5G07100 39.130 138 73 4 139 273 81 210 1.79e-25 103
MS.gene025402.t1 AT5G07100 39.130 138 73 4 139 273 81 210 1.79e-25 103
MS.gene025402.t1 AT5G07100 39.130 138 73 4 139 273 81 210 1.79e-25 103
MS.gene025402.t1 AT5G07100 39.130 138 73 4 139 273 81 210 1.79e-25 103
MS.gene025402.t1 AT5G07100 38.571 140 75 4 137 273 172 303 2.64e-25 105
MS.gene025402.t1 AT1G80840 31.658 199 106 6 68 264 36 206 1.80e-22 97.4
MS.gene025402.t1 AT2G38470 42.857 119 64 3 149 266 308 423 4.76e-22 98.6
MS.gene025402.t1 AT4G31800 56.000 75 32 1 198 271 104 178 8.63e-22 94.4
MS.gene025402.t1 AT2G47260 48.810 84 42 1 188 271 158 240 8.89e-22 95.9
MS.gene025402.t1 AT4G31800 56.000 75 32 1 198 271 169 243 9.25e-22 95.5
MS.gene025402.t1 AT1G29860 48.148 81 41 1 188 268 120 199 1.69e-21 94.4
MS.gene025402.t1 AT2G30250 47.826 92 45 2 179 268 301 391 2.47e-21 95.5
MS.gene025402.t1 AT5G41570 48.352 91 42 2 175 261 65 154 3.14e-21 90.9
MS.gene025402.t1 AT2G04880 49.351 77 38 1 190 266 269 344 4.41e-21 95.5
MS.gene025402.t1 AT2G04880 49.351 77 38 1 190 266 293 368 5.43e-21 95.5
MS.gene025402.t1 AT4G18170 45.714 105 51 4 162 264 131 231 7.08e-21 93.2
MS.gene025402.t1 AT2G25000 55.556 72 31 1 197 267 95 166 7.71e-21 91.3
MS.gene025402.t1 AT2G25000 55.556 72 31 1 197 267 95 166 7.71e-21 91.3
MS.gene025402.t1 AT2G25000 55.556 72 31 1 197 267 117 188 1.40e-20 90.9
MS.gene025402.t1 AT2G25000 55.556 72 31 1 197 267 138 209 1.70e-20 91.3
MS.gene025402.t1 AT5G56270 48.864 88 44 1 179 266 462 548 3.71e-20 93.6
MS.gene025402.t1 AT4G26640 49.351 77 38 1 188 264 189 264 4.06e-20 92.0
MS.gene025402.t1 AT4G26640 49.351 77 38 1 188 264 189 264 4.06e-20 92.0
MS.gene025402.t1 AT4G26640 49.351 77 38 1 188 264 189 264 4.06e-20 92.0
MS.gene025402.t1 AT4G26640 49.351 77 38 1 188 264 189 264 4.06e-20 92.0
MS.gene025402.t1 AT1G64000 51.190 84 37 2 191 274 101 180 4.29e-20 88.2
MS.gene025402.t1 AT4G26640 48.718 78 39 1 187 264 324 400 6.05e-20 92.4
MS.gene025402.t1 AT4G26640 48.718 78 39 1 187 264 292 368 7.07e-20 92.0
MS.gene025402.t1 AT4G26640 49.351 77 38 1 188 264 365 440 8.04e-20 92.0
MS.gene025402.t1 AT1G13960 50.000 74 36 1 191 264 243 315 1.54e-19 89.7
MS.gene025402.t1 AT1G13960 50.000 60 28 2 205 264 77 134 1.11e-11 66.2
MS.gene025402.t1 AT1G13960 50.000 74 36 1 191 264 396 468 1.97e-19 90.9
MS.gene025402.t1 AT1G13960 50.000 60 28 2 205 264 230 287 7.20e-12 67.4
MS.gene025402.t1 AT5G26170 51.389 72 34 1 190 261 99 169 3.43e-19 85.1
MS.gene025402.t1 AT3G62340 44.037 109 55 2 191 294 105 212 3.48e-19 87.4
MS.gene025402.t1 AT2G03340 48.684 76 38 1 191 266 402 476 3.55e-19 90.1
MS.gene025402.t1 AT2G03340 51.667 60 27 2 205 264 251 308 6.80e-13 70.9
MS.gene025402.t1 AT5G46350 41.667 96 54 2 191 286 170 263 4.20e-19 88.2
MS.gene025402.t1 AT5G49520 45.161 93 49 2 188 279 205 296 5.04e-19 89.0
MS.gene025402.t1 AT2G46130 45.263 95 48 2 180 274 6 96 5.49e-19 82.4
MS.gene025402.t1 AT5G13080 48.193 83 39 2 191 273 54 132 6.16e-19 83.6
MS.gene025402.t1 AT5G64810 48.276 87 41 2 176 262 85 167 6.23e-19 84.7
MS.gene025402.t1 AT3G01080 48.649 74 37 1 191 264 293 365 1.16e-18 87.8
MS.gene025402.t1 AT3G01080 48.649 74 37 1 191 264 331 403 1.19e-18 88.2
MS.gene025402.t1 AT1G69310 45.238 84 45 1 179 262 122 204 1.67e-18 85.9
MS.gene025402.t1 AT1G69310 45.238 84 45 1 179 262 122 204 1.67e-18 85.9
MS.gene025402.t1 AT1G69310 45.238 84 45 1 179 262 122 204 1.67e-18 85.9
MS.gene025402.t1 AT3G01970 50.000 74 36 1 190 263 51 123 1.74e-18 82.4
MS.gene025402.t1 AT4G26440 34.459 148 83 5 154 288 311 457 2.03e-18 87.8
MS.gene025402.t1 AT4G30935 38.136 118 67 3 175 288 303 418 8.97e-18 85.5
MS.gene025402.t1 AT2G23320 57.627 59 25 0 205 263 241 299 1.16e-17 84.0
MS.gene025402.t1 AT2G37260 39.695 131 59 6 150 267 211 334 1.37e-17 84.0
MS.gene025402.t1 AT2G37260 44.156 77 39 3 204 280 85 157 2.38e-13 71.2
MS.gene025402.t1 AT2G37260 39.695 131 59 6 150 267 209 332 1.41e-17 84.0
MS.gene025402.t1 AT2G37260 44.156 77 39 3 204 280 83 155 2.44e-13 71.2
MS.gene025402.t1 AT2G37260 41.221 131 57 7 150 267 291 414 2.25e-17 84.0
MS.gene025402.t1 AT2G37260 44.156 77 39 3 204 280 165 237 3.16e-13 71.6
MS.gene025402.t1 AT5G28650 57.895 57 24 0 205 261 263 319 5.22e-17 82.0
MS.gene025402.t1 AT2G30590 54.688 64 28 1 205 267 314 377 5.30e-17 82.8
MS.gene025402.t1 AT3G04670 57.895 57 24 0 205 261 263 319 7.89e-17 81.6
MS.gene025402.t1 AT4G24240 55.932 59 26 0 205 263 282 340 9.74e-17 81.6
MS.gene025402.t1 AT1G29280 49.333 75 30 1 205 279 75 141 3.08e-16 78.6
MS.gene025402.t1 AT4G31550 44.872 78 40 1 205 282 247 321 3.14e-16 79.7
MS.gene025402.t1 AT4G31550 44.872 78 40 1 205 282 246 320 3.32e-16 79.7
MS.gene025402.t1 AT2G44745 43.956 91 46 2 175 261 112 201 3.63e-16 77.4
MS.gene025402.t1 AT1G55600 42.697 89 46 2 182 266 281 368 3.82e-16 80.5
MS.gene025402.t1 AT2G24570 53.448 58 27 0 205 262 244 301 7.47e-16 78.6
MS.gene025402.t1 AT1G30650 43.529 85 47 1 181 264 193 277 1.47e-15 78.6
MS.gene025402.t1 AT4G01250 36.957 138 62 3 201 338 125 237 1.57e-15 77.4
MS.gene025402.t1 AT2G34830 43.529 85 47 1 181 264 191 275 1.66e-15 78.6
MS.gene025402.t1 AT3G58710 55.000 60 27 0 205 264 72 131 2.09e-15 76.6
MS.gene025402.t1 AT2G34830 43.529 85 47 1 181 264 200 284 2.10e-15 78.2
MS.gene025402.t1 AT3G58710 55.000 60 27 0 205 264 71 130 2.16e-15 76.3
MS.gene025402.t1 AT4G23550 43.373 83 47 0 202 284 138 220 2.42e-15 77.0
MS.gene025402.t1 AT5G52830 50.000 70 35 0 205 274 166 235 1.28e-14 75.1
MS.gene025402.t1 AT2G46130 42.105 95 47 3 180 274 6 92 3.87e-14 68.6
MS.gene025402.t1 AT4G39410 40.000 85 50 1 177 261 196 279 4.42e-14 73.2
MS.gene025402.t1 AT1G69310 41.667 84 44 2 179 262 122 200 1.22e-13 71.2
MS.gene025402.t1 AT5G45050 36.842 114 69 1 205 315 1181 1294 2.74e-13 72.8
MS.gene025402.t1 AT5G45050 36.842 114 69 1 205 315 1042 1155 2.74e-13 72.4
MS.gene025402.t1 AT5G45050 36.842 114 69 1 205 315 1153 1266 2.80e-13 72.4
MS.gene025402.t1 AT4G31800 62.222 45 16 1 198 241 169 213 4.22e-12 66.2
MS.gene025402.t1 AT5G43290 47.541 61 31 1 205 265 115 174 4.46e-12 66.6
MS.gene025402.t1 AT2G21900 46.667 60 31 1 205 264 110 168 4.91e-12 65.1
MS.gene025402.t1 AT2G46400 37.000 100 61 1 183 280 83 182 6.92e-12 66.2
MS.gene025402.t1 AT3G01080 46.667 60 30 2 205 264 206 263 8.51e-11 63.5
MS.gene025402.t1 AT2G40740 42.683 82 36 3 205 275 174 255 9.73e-11 62.8

Find 93 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCATTGTTTATCATATT+TGG 0.055350 2.2:+41997131 None:intergenic
CACTACTCTTACTAGGTAAT+TGG 0.189983 2.2:+41996985 None:intergenic
ATTAATCACTTACTTGGATT+TGG 0.221328 2.2:+42000288 None:intergenic
TTAATCACTTACTTGGATTT+GGG 0.256367 2.2:+42000289 None:intergenic
GAAAACTTACAGTATTCTTA+TGG 0.285405 2.2:+42000752 None:intergenic
TCTTCAGAATCGTTTACTTT+TGG 0.317553 2.2:-41999971 MS.gene025402:CDS
ATGATTATGGTTTCCTTCAT+AGG 0.322830 2.2:+41997463 None:intergenic
AGCCTACTATCGTTGCAGTA+TGG 0.326812 2.2:-41999010 MS.gene025402:CDS
GGTTGTCAATGGAGAAAATA+TGG 0.329418 2.2:-41999066 MS.gene025402:CDS
GGGGATTTCCTTGAATTTGA+TGG 0.332291 2.2:+42000309 None:intergenic
AAAATCTGATCGTGCCCTTA+TGG 0.343745 2.2:+41999226 None:intergenic
TAGTTTACCTGCTTACGAAC+TGG 0.359165 2.2:+41998976 None:intergenic
TCTTTATTGTTATTATCATA+TGG 0.359665 2.2:+42000808 None:intergenic
GTAGCTATAGAATTTGAGTT+TGG 0.360803 2.2:+41997299 None:intergenic
TCAACAAATTTACAACAATA+TGG 0.363714 2.2:-41997368 MS.gene025402:CDS
GAAGTTGTGTTGTGTTATTA+AGG 0.369494 2.2:+42000333 None:intergenic
TCTTACTAGGTAATTGGCTT+TGG 0.372010 2.2:+41996991 None:intergenic
TTTGAGTTTGGTGGAGATAA+TGG 0.375274 2.2:+41997311 None:intergenic
AATTAACCACTACTCTTACT+AGG 0.379801 2.2:+41996978 None:intergenic
GATGATGTTAAGTTTGCTAT+TGG 0.388021 2.2:+41997419 None:intergenic
TTTAAGAGGTATGCTAAATC+TGG 0.393699 2.2:-42000013 MS.gene025402:CDS
CTGGTGGAAGTTGATGATTA+TGG 0.407669 2.2:+41997450 None:intergenic
CAATGAGAGAAAACTTGCTT+TGG 0.409723 2.2:-41997117 MS.gene025402:CDS
GCTATAGAATTTGAGTTTGG+TGG 0.412545 2.2:+41997302 None:intergenic
AGTTCAAACAATGAAATTGC+TGG 0.417191 2.2:-42000848 None:intergenic
TGTAATGTTTAGATGGTTGA+TGG 0.426715 2.2:-41999087 MS.gene025402:intron
CGATAGTAGGCTCGTGGACA+AGG 0.430122 2.2:+41999021 None:intergenic
GTAAAATCAAGTGTAATTGT+AGG 0.435039 2.2:+41997254 None:intergenic
GGAGAGCTAGAGTGTCCATA+AGG 0.442520 2.2:-41999241 MS.gene025402:CDS
GGGATTTCCTTGAATTTGAT+GGG 0.451310 2.2:+42000310 None:intergenic
AAATAATGGATTTGCCATAG+TGG 0.457421 2.2:-41999366 MS.gene025402:CDS
TATTGACCTTGGAATGGAAA+AGG 0.459010 2.2:+41997176 None:intergenic
TGAAATAAATAGCAAAATAG+AGG 0.463782 2.2:-41999288 MS.gene025402:CDS
AAGGAGAATGAAAATTTAAG+AGG 0.466438 2.2:-42000027 MS.gene025402:CDS
CAACTTCCCCATCAAATTCA+AGG 0.467550 2.2:-42000317 MS.gene025402:CDS
GCCTACTATCGTTGCAGTAT+GGG 0.470435 2.2:-41999009 MS.gene025402:CDS
TGCACCCAACCTCAGATGAT+TGG 0.470879 2.2:+41999264 None:intergenic
TTGAATTGCAAATAGTTACT+TGG 0.470909 2.2:+41997224 None:intergenic
ACAGGTGCAAAGGTGTTTCA+AGG 0.472939 2.2:-41997507 MS.gene025402:intron
GCCATGTCCTCGTTCACAAA+TGG 0.479556 2.2:-41997035 MS.gene025402:CDS
TTTGGAAACACCTCAAATGA+TGG 0.495575 2.2:+41997149 None:intergenic
AGTTTACCTGCTTACGAACT+GGG 0.502879 2.2:+41998977 None:intergenic
AGATGGTTGATGGTTGTCAA+TGG 0.503311 2.2:-41999077 MS.gene025402:CDS
CCCCATACTGCAACGATAGT+AGG 0.503349 2.2:+41999008 None:intergenic
GAAGCTTGATGAAGCAAAGA+AGG 0.508309 2.2:-42000046 MS.gene025402:CDS
AGGACCAATCATCTGAGGTT+GGG 0.511884 2.2:-41999268 MS.gene025402:CDS
GAGAGCTAGAGTGTCCATAA+GGG 0.513998 2.2:-41999240 MS.gene025402:CDS
TTCATGCCCAGTTCGTAAGC+AGG 0.514284 2.2:-41998983 MS.gene025402:intron
CTTAATAAAAGAGTAGATCC+TGG 0.516100 2.2:+42000362 None:intergenic
TGTAAATTTGTTGATGAGGT+AGG 0.517802 2.2:+41997377 None:intergenic
AGTATTCTTATGGAAGGAAG+AGG 0.520852 2.2:+42000762 None:intergenic
TAAAATCAAGTGTAATTGTA+GGG 0.521053 2.2:+41997255 None:intergenic
AAATAATCACAACTTGCTGC+TGG 0.523498 2.2:-41999914 MS.gene025402:intron
GAGGACCAATCATCTGAGGT+TGG 0.528239 2.2:-41999269 MS.gene025402:CDS
TCGTGGACAAGGGTTGCCCT+TGG 0.531338 2.2:+41999032 None:intergenic
ATTGTAGGGCATGTTGGAGA+TGG 0.532259 2.2:+41997269 None:intergenic
AGTGTAATTGTAGGGCATGT+TGG 0.533130 2.2:+41997263 None:intergenic
AATCATCTGAGGTTGGGTGC+AGG 0.533609 2.2:-41999262 MS.gene025402:CDS
TTGCTACAATCTGAAGGTGA+AGG 0.539687 2.2:+41999389 None:intergenic
GAGATAATGGTGAAGAGAAG+AGG 0.540861 2.2:+41997324 None:intergenic
AATTGCAAATAGTTACTTGG+AGG 0.545616 2.2:+41997227 None:intergenic
TCCATTTGTGAACGAGGACA+TGG 0.553076 2.2:+41997034 None:intergenic
GTGTTCATCACAACCTATGA+AGG 0.556151 2.2:-41997476 MS.gene025402:CDS
ATGATGGAGGGTATTGACCT+TGG 0.561075 2.2:+41997165 None:intergenic
ATTGACCTTGGAATGGAAAA+GGG 0.562633 2.2:+41997177 None:intergenic
ATTCCAATTAACAGGTGCAA+AGG 0.570557 2.2:-41997517 MS.gene025402:intron
TCAATACCCTCCATCATTTG+AGG 0.572138 2.2:-41997159 MS.gene025402:CDS
AACTGACGAGTAGGTAACAC+TGG 0.572998 2.2:+41999422 None:intergenic
ACTTACAGTATTCTTATGGA+AGG 0.574288 2.2:+42000756 None:intergenic
AATTTGTTGATGAGGTAGGA+AGG 0.579387 2.2:+41997381 None:intergenic
TAATCACTTACTTGGATTTG+GGG 0.580090 2.2:+42000290 None:intergenic
AATAATGCTTCCTTGAGAGA+AGG 0.583998 2.2:+41997062 None:intergenic
ATTGCAAATAGTTACTTGGA+GGG 0.585471 2.2:+41997228 None:intergenic
CCTACTATCGTTGCAGTATG+GGG 0.587972 2.2:-41999008 MS.gene025402:CDS
TTATTGTTATTATCATATGG+TGG 0.589100 2.2:+42000811 None:intergenic
GCATGTGAATACATTAATGA+AGG 0.599725 2.2:-41999311 MS.gene025402:CDS
GGAAACACCTCAAATGATGG+AGG 0.600501 2.2:+41997152 None:intergenic
GGAGGGTATTGACCTTGGAA+TGG 0.604564 2.2:+41997170 None:intergenic
GAAGGGAAATACACAAGATG+CGG 0.615352 2.2:-41999450 MS.gene025402:CDS
TCATCAAAGAACTGACGAGT+AGG 0.619422 2.2:+41999413 None:intergenic
TATGGACAAAAGACAGCCAA+GGG 0.623915 2.2:-41999048 MS.gene025402:CDS
ATATGGACAAAAGACAGCCA+AGG 0.629423 2.2:-41999049 MS.gene025402:CDS
TTGTTGTAAATTTGTTGATG+AGG 0.630686 2.2:+41997373 None:intergenic
CTGCAACGATAGTAGGCTCG+TGG 0.637106 2.2:+41999015 None:intergenic
CAATTACCTAGTAAGAGTAG+TGG 0.638666 2.2:-41996984 MS.gene025402:CDS
GAAACACCTCAAATGATGGA+GGG 0.639752 2.2:+41997153 None:intergenic
GATAGTAGGCTCGTGGACAA+GGG 0.644110 2.2:+41999022 None:intergenic
GGAAAACAATGAAAACAACA+TGG 0.645971 2.2:-41999345 MS.gene025402:CDS
GGATTTCCTTGAATTTGATG+GGG 0.651214 2.2:+42000311 None:intergenic
AATAGAGGACCAATCATCTG+AGG 0.655874 2.2:-41999273 MS.gene025402:CDS
AGAATCTCCATTTGTGAACG+AGG 0.664014 2.2:+41997028 None:intergenic
GAAAACAATGAAAACAACAT+GGG 0.672904 2.2:-41999344 MS.gene025402:CDS
AATTGCTGGAAGTTGATGAA+AGG 0.676322 2.2:-42000834 MS.gene025402:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATCAAATATCAAATA+TGG + chr2.2:41997126-41997145 None:intergenic 10.0%
!!! AATTTTAATGCATTAAAAAA+GGG - chr2.2:41999345-41999364 MS.gene025402:CDS 10.0%
!!! AATTTTTTTTAGAATTTGTT+GGG + chr2.2:41999029-41999048 None:intergenic 10.0%
!!! GATAAATTAAACTTTAATTT+TGG - chr2.2:41998236-41998255 MS.gene025402:intron 10.0%
!!! TAATTTTTTTTAGAATTTGT+TGG + chr2.2:41999030-41999049 None:intergenic 10.0%
!! TTCTTCATAATTAATTAGAA+GGG - chr2.2:41998351-41998370 MS.gene025402:intron 15.0%
!! TTTCTTCATAATTAATTAGA+AGG - chr2.2:41998350-41998369 MS.gene025402:intron 15.0%
!!! ATTAATTTGTAATGTTTAGA+TGG - chr2.2:41998724-41998743 MS.gene025402:intron 15.0%
!!! ATTTTAAAAAAGAATTACAC+CGG + chr2.2:42000221-42000240 None:intergenic 15.0%
!!! ATTTTAATGCATTAAAAAAG+GGG - chr2.2:41999346-41999365 MS.gene025402:CDS 15.0%
!!! ATTTTCAAATTTTTTGAACA+TGG - chr2.2:41999603-41999622 MS.gene025402:intron 15.0%
!!! CAATTTTAATGCATTAAAAA+AGG - chr2.2:41999344-41999363 MS.gene025402:CDS 15.0%
!!! TCTTTATTGTTATTATCATA+TGG + chr2.2:41997013-41997032 None:intergenic 15.0%
!!! TTAAACTTTAATTTTGGATT+TGG - chr2.2:41998242-41998261 MS.gene025402:intron 15.0%
!!! TTTTCAAATTTTTTGAACAT+GGG - chr2.2:41999604-41999623 MS.gene025402:intron 15.0%
!! AATAAGATTAATCACTTACT+TGG + chr2.2:41997539-41997558 None:intergenic 20.0%
!! ATTTAATGATCTCATGTATT+TGG - chr2.2:41998008-41998027 MS.gene025402:intron 20.0%
!! TAAAATCAAGTGTAATTGTA+GGG + chr2.2:42000566-42000585 None:intergenic 20.0%
!! TAGGTAATATCATATGAAAA+TGG + chr2.2:41998890-41998909 None:intergenic 20.0%
!! TATGAAGAAAAAATGTACAA+AGG + chr2.2:41998340-41998359 None:intergenic 20.0%
!! TCAACAAATTTACAACAATA+TGG - chr2.2:42000450-42000469 MS.gene025402:intron 20.0%
!! TGAAATAAATAGCAAAATAG+AGG - chr2.2:41998530-41998549 MS.gene025402:intron 20.0%
!! TTAATTACATTCCAATTAAC+AGG - chr2.2:42000293-42000312 MS.gene025402:intron 20.0%
!! TTTATCTATTTGTAACTAGA+CGG + chr2.2:41998087-41998106 None:intergenic 20.0%
!!! ATAAGGATGACAATATTTTA+AGG - chr2.2:42000029-42000048 MS.gene025402:CDS 20.0%
!!! GGTGTAATTCTTTTTTAAAA+TGG - chr2.2:42000220-42000239 MS.gene025402:intron 20.0%
!!! TAATAACTTGACATTTTGAT+GGG - chr2.2:41997421-41997440 MS.gene025402:CDS 20.0%
!!! TACAACTTATGATTGTTTTT+AGG + chr2.2:41998909-41998928 None:intergenic 20.0%
!!! TGTTGTTTATGTAATTAACA+TGG - chr2.2:41998162-41998181 MS.gene025402:intron 20.0%
!!! TTACAAGTTGAAAATATCAT+AGG + chr2.2:41998295-41998314 None:intergenic 20.0%
!!! TTATTGTTATTATCATATGG+TGG + chr2.2:41997010-41997029 None:intergenic 20.0%
!!! TTTCAAATTTTTTGAACATG+GGG - chr2.2:41999605-41999624 MS.gene025402:intron 20.0%
!!! TTTTAAGGAACAAATTATTG+TGG - chr2.2:42000044-42000063 MS.gene025402:CDS 20.0%
!!! TTTTTAAAATGGTGTGTATA+TGG - chr2.2:42000231-42000250 MS.gene025402:intron 20.0%
! AAATGAGTGTGAATCAAATA+AGG + chr2.2:41997961-41997980 None:intergenic 25.0%
! AAATGTCAAGTTATTAGGTT+AGG + chr2.2:41997417-41997436 None:intergenic 25.0%
! AAGGAGAATGAAAATTTAAG+AGG - chr2.2:41997791-41997810 MS.gene025402:intron 25.0%
! ATTAATCACTTACTTGGATT+TGG + chr2.2:41997533-41997552 None:intergenic 25.0%
! CATCAAAATGTCAAGTTATT+AGG + chr2.2:41997422-41997441 None:intergenic 25.0%
! CTCTCATTGTTTATCATATT+TGG + chr2.2:42000690-42000709 None:intergenic 25.0%
! GAAAACAATGAAAACAACAT+GGG - chr2.2:41998474-41998493 MS.gene025402:intron 25.0%
! GAAAACTTACAGTATTCTTA+TGG + chr2.2:41997069-41997088 None:intergenic 25.0%
! GAACAAAACTTACTATACAT+AGG - chr2.2:41999963-41999982 MS.gene025402:CDS 25.0%
! GATAATGCATATTATATGCA+GGG + chr2.2:42000169-42000188 None:intergenic 25.0%
! GTAAAATCAAGTGTAATTGT+AGG + chr2.2:42000567-42000586 None:intergenic 25.0%
! TATCAAAGCTCAAATAAACA+AGG + chr2.2:41999735-41999754 None:intergenic 25.0%
! TCAGATTGTAGCAAAAATAA+TGG - chr2.2:41998438-41998457 MS.gene025402:intron 25.0%
! TTAATCACTTACTTGGATTT+GGG + chr2.2:41997532-41997551 None:intergenic 25.0%
! TTAGCTTATGATGAAAATCA+CGG - chr2.2:41997327-41997346 MS.gene025402:CDS 25.0%
! TTATCTATTTGTAACTAGAC+GGG + chr2.2:41998086-41998105 None:intergenic 25.0%
! TTGAATTGCAAATAGTTACT+TGG + chr2.2:42000597-42000616 None:intergenic 25.0%
! TTGAGCAAATATGTTGTTAT+GGG - chr2.2:41999264-41999283 MS.gene025402:CDS 25.0%
! TTTGAGCAAATATGTTGTTA+TGG - chr2.2:41999263-41999282 MS.gene025402:CDS 25.0%
!! AAGAGTCATATACTTTGTTT+AGG - chr2.2:41997220-41997239 MS.gene025402:CDS 25.0%
!! ATTCTCAGTTTCATTCTTTT+AGG + chr2.2:41999771-41999790 None:intergenic 25.0%
!! CTAATAACTTGACATTTTGA+TGG - chr2.2:41997420-41997439 MS.gene025402:CDS 25.0%
!!! TTATTTTTGCTACAATCTGA+AGG + chr2.2:41998438-41998457 None:intergenic 25.0%
!!! TTGTTGTAAATTTGTTGATG+AGG + chr2.2:42000448-42000467 None:intergenic 25.0%
!!! TTTGATAATTGCTCTAAGAT+TGG - chr2.2:41997737-41997756 MS.gene025402:intron 25.0%
AATTGCAAATAGTTACTTGG+AGG + chr2.2:42000594-42000613 None:intergenic 30.0%
ACTTACAGTATTCTTATGGA+AGG + chr2.2:41997065-41997084 None:intergenic 30.0%
ATGATTATGGTTTCCTTCAT+AGG + chr2.2:42000358-42000377 None:intergenic 30.0%
ATTGCAAATAGTTACTTGGA+GGG + chr2.2:42000593-42000612 None:intergenic 30.0%
CTTAATAAAAGAGTAGATCC+TGG + chr2.2:41997459-41997478 None:intergenic 30.0%
GCATGTGAATACATTAATGA+AGG - chr2.2:41998507-41998526 MS.gene025402:intron 30.0%
GCTATAGAAATTCACCTTAA+AGG - chr2.2:42000109-42000128 MS.gene025402:intron 30.0%
GGAAAACAATGAAAACAACA+TGG - chr2.2:41998473-41998492 MS.gene025402:intron 30.0%
GGATAATGCATATTATATGC+AGG + chr2.2:42000170-42000189 None:intergenic 30.0%
TAATCACTTACTTGGATTTG+GGG + chr2.2:41997531-41997550 None:intergenic 30.0%
TAATCCAGTATACACAAACA+TGG + chr2.2:41997099-41997118 None:intergenic 30.0%
TAGCTTATGATGAAAATCAC+GGG - chr2.2:41997328-41997347 MS.gene025402:CDS 30.0%
TCCAGAACTAACTATTGTAT+AGG + chr2.2:41999804-41999823 None:intergenic 30.0%
TCCTATACAATAGTTAGTTC+TGG - chr2.2:41999800-41999819 MS.gene025402:intron 30.0%
TGAAATGAAACCAGAAACAA+GGG - chr2.2:41999697-41999716 MS.gene025402:intron 30.0%
TGTAATGTTTAGATGGTTGA+TGG - chr2.2:41998731-41998750 MS.gene025402:intron 30.0%
TTAATGCATTAAAAAAGGGG+AGG - chr2.2:41999349-41999368 MS.gene025402:CDS 30.0%
TTGAAATGAAACCAGAAACA+AGG - chr2.2:41999696-41999715 MS.gene025402:intron 30.0%
TTGTAGTGCATCATCAATTA+AGG + chr2.2:41999311-41999330 None:intergenic 30.0%
! GAAGTTGTGTTGTGTTATTA+AGG + chr2.2:41997488-41997507 None:intergenic 30.0%
! GATCAGATTTTGCATTTGTA+AGG - chr2.2:41998604-41998623 MS.gene025402:intron 30.0%
! GTAGCTATAGAATTTGAGTT+TGG + chr2.2:42000522-42000541 None:intergenic 30.0%
! GTTTTCATTGTTTTCCACTA+TGG + chr2.2:41998469-41998488 None:intergenic 30.0%
! TCTTCAGAATCGTTTACTTT+TGG - chr2.2:41997847-41997866 MS.gene025402:intron 30.0%
! TTTAAGAGGTATGCTAAATC+TGG - chr2.2:41997805-41997824 MS.gene025402:intron 30.0%
!! AAATAATGGATTTGCCATAG+TGG - chr2.2:41998452-41998471 MS.gene025402:intron 30.0%
!! AGCAAGTTGTGATTATTTTG+AGG + chr2.2:41997902-41997921 None:intergenic 30.0%
!! GATGATGTTAAGTTTGCTAT+TGG + chr2.2:42000402-42000421 None:intergenic 30.0%
!! TGTAAATTTGTTGATGAGGT+AGG + chr2.2:42000444-42000463 None:intergenic 30.0%
!!! AATTTTGGATTTGGTGAATG+TGG - chr2.2:41998251-41998270 MS.gene025402:intron 30.0%
!!! TTCTTTTAGGTTGAATGTGT+AGG + chr2.2:41999758-41999777 None:intergenic 30.0%
!!! TTTTTTTTCAAGTAGCCTAG+CGG - chr2.2:42000087-42000106 MS.gene025402:intron 30.0%
AAAGAATGCTACCAAAGAGA+TGG - chr2.2:41999471-41999490 MS.gene025402:intron 35.0%
AAAGTTTGTGAAAGGAAAGG+TGG - chr2.2:41999524-41999543 MS.gene025402:intron 35.0%
AACTTTCACCTCCATTTAGT+TGG + chr2.2:41999510-41999529 None:intergenic 35.0%
AAGTTTGTGAAAGGAAAGGT+GGG - chr2.2:41999525-41999544 MS.gene025402:intron 35.0%
AATAATGCTTCCTTGAGAGA+AGG + chr2.2:42000759-42000778 None:intergenic 35.0%
ACCTTAAAGGTCAATATGTG+GGG - chr2.2:42000122-42000141 MS.gene025402:intron 35.0%
AGAAAAAGATCCTTCTCTCA+AGG - chr2.2:42000746-42000765 MS.gene025402:intron 35.0%
AGTATTCTTATGGAAGGAAG+AGG + chr2.2:41997059-41997078 None:intergenic 35.0%
ATGCATATTATATGCAGGGA+TGG + chr2.2:42000165-42000184 None:intergenic 35.0%
ATTCCAATTAACAGGTGCAA+AGG - chr2.2:42000301-42000320 MS.gene025402:CDS 35.0%
ATTGACCTTGGAATGGAAAA+GGG + chr2.2:42000644-42000663 None:intergenic 35.0%
CAATTACCTAGTAAGAGTAG+TGG - chr2.2:42000834-42000853 MS.gene025402:CDS 35.0%
CACCTTAAAGGTCAATATGT+GGG - chr2.2:42000121-42000140 MS.gene025402:intron 35.0%
CACTACTCTTACTAGGTAAT+TGG + chr2.2:42000836-42000855 None:intergenic 35.0%
CGGACAGACTATTTATTTGA+GGG - chr2.2:41999127-41999146 MS.gene025402:intron 35.0%
GGACAGACTATTTATTTGAG+GGG - chr2.2:41999128-41999147 MS.gene025402:intron 35.0%
GGATTTCCTTGAATTTGATG+GGG + chr2.2:41997510-41997529 None:intergenic 35.0%
GGGATTTCCTTGAATTTGAT+GGG + chr2.2:41997511-41997530 None:intergenic 35.0%
GGTTGTCAATGGAGAAAATA+TGG - chr2.2:41998752-41998771 MS.gene025402:intron 35.0%
GTGAAAGTTTGTGAAAGGAA+AGG - chr2.2:41999521-41999540 MS.gene025402:intron 35.0%
TAGTGTTGTCGATCATCAAT+TGG - chr2.2:41998196-41998215 MS.gene025402:intron 35.0%
TATGCCATGTTTGTGTATAC+TGG - chr2.2:41997092-41997111 MS.gene025402:CDS 35.0%
TATTGACCTTGGAATGGAAA+AGG + chr2.2:42000645-42000664 None:intergenic 35.0%
TCACCTTAAAGGTCAATATG+TGG - chr2.2:42000120-42000139 MS.gene025402:intron 35.0%
TGCAAGCTCTCTTCAAATTT+AGG - chr2.2:41999377-41999396 MS.gene025402:CDS 35.0%
TGCATATTATATGCAGGGAT+GGG + chr2.2:42000164-42000183 None:intergenic 35.0%
TTTGGAAACACCTCAAATGA+TGG + chr2.2:42000672-42000691 None:intergenic 35.0%
! ATCTCTTTGGTAGCATTCTT+TGG + chr2.2:41999472-41999491 None:intergenic 35.0%
! CAATGAGAGAAAACTTGCTT+TGG - chr2.2:42000701-42000720 MS.gene025402:intron 35.0%
! GAGTTTAGCTCACTTGATAA+GGG + chr2.2:42000191-42000210 None:intergenic 35.0%
! GCTATAGAATTTGAGTTTGG+TGG + chr2.2:42000519-42000538 None:intergenic 35.0%
! TCGATTTTATAGAAGCGAGT+TGG - chr2.2:41998955-41998974 MS.gene025402:intron 35.0%
! TCTTACTAGGTAATTGGCTT+TGG + chr2.2:42000830-42000849 None:intergenic 35.0%
! TGAGTTTAGCTCACTTGATA+AGG + chr2.2:42000192-42000211 None:intergenic 35.0%
! TTTGAGTTTGGTGGAGATAA+TGG + chr2.2:42000510-42000529 None:intergenic 35.0%
!! AAATAATCACAACTTGCTGC+TGG - chr2.2:41997904-41997923 MS.gene025402:intron 35.0%
!! AATTGCTGGAAGTTGATGAA+AGG - chr2.2:41996984-41997003 MS.gene025402:CDS 35.0%
!! AATTTGTTGATGAGGTAGGA+AGG + chr2.2:42000440-42000459 None:intergenic 35.0%
!!! GTTGTGATTATTTTGAGGCA+AGG + chr2.2:41997897-41997916 None:intergenic 35.0%
AAAATCTGATCGTGCCCTTA+TGG + chr2.2:41998595-41998614 None:intergenic 40.0%
AATAGAGGACCAATCATCTG+AGG - chr2.2:41998545-41998564 MS.gene025402:intron 40.0%
ACACCTTTGCACCTGTTAAT+TGG + chr2.2:42000307-42000326 None:intergenic 40.0%
ACCCCACATATTGACCTTTA+AGG + chr2.2:42000126-42000145 None:intergenic 40.0%
AGAATCTCCATTTGTGAACG+AGG + chr2.2:42000793-42000812 None:intergenic 40.0%
AGTGTAATTGTAGGGCATGT+TGG + chr2.2:42000558-42000577 None:intergenic 40.0%
AGTTTACCTGCTTACGAACT+GGG + chr2.2:41998844-41998863 None:intergenic 40.0%
ATATGGACAAAAGACAGCCA+AGG - chr2.2:41998769-41998788 MS.gene025402:intron 40.0%
ATGAAACCAGAAACAAGGGA+AGG - chr2.2:41999701-41999720 MS.gene025402:intron 40.0%
CAACTTCCCCATCAAATTCA+AGG - chr2.2:41997501-41997520 MS.gene025402:CDS 40.0%
CCTCTCTATTGAGAATGATG+CGG - chr2.2:41999107-41999126 MS.gene025402:intron 40.0%
GAAGCTTGATGAAGCAAAGA+AGG - chr2.2:41997772-41997791 MS.gene025402:intron 40.0%
GAAGGGAAATACACAAGATG+CGG - chr2.2:41998368-41998387 MS.gene025402:intron 40.0%
GACTCTTGGTTCCAACTAAA+TGG - chr2.2:41999496-41999515 MS.gene025402:intron 40.0%
GCATATTATATGCAGGGATG+GGG + chr2.2:42000163-42000182 None:intergenic 40.0%
GCGGACAGACTATTTATTTG+AGG - chr2.2:41999126-41999145 MS.gene025402:intron 40.0%
GGGGATTTCCTTGAATTTGA+TGG + chr2.2:41997512-41997531 None:intergenic 40.0%
GTGTTCATCACAACCTATGA+AGG - chr2.2:42000342-42000361 MS.gene025402:CDS 40.0%
GTTGTCGATCATCAATTGGT+TGG - chr2.2:41998200-41998219 MS.gene025402:intron 40.0%
TAGTTTACCTGCTTACGAAC+TGG + chr2.2:41998845-41998864 None:intergenic 40.0%
TATGATGAAAATCACGGGTG+TGG - chr2.2:41997333-41997352 MS.gene025402:CDS 40.0%
TATGGACAAAAGACAGCCAA+GGG - chr2.2:41998770-41998789 MS.gene025402:intron 40.0%
TCAAGTGAGCTAAACTCACA+TGG - chr2.2:42000194-42000213 MS.gene025402:intron 40.0%
TCAATACCCTCCATCATTTG+AGG - chr2.2:42000659-42000678 MS.gene025402:intron 40.0%
TCATCAAAGAACTGACGAGT+AGG + chr2.2:41998408-41998427 None:intergenic 40.0%
TCTTGGTTCCAACTAAATGG+AGG - chr2.2:41999499-41999518 MS.gene025402:intron 40.0%
TGGAGGTGAAAGTTTGTGAA+AGG - chr2.2:41999516-41999535 MS.gene025402:intron 40.0%
TGTATGAATCGAGATCATCG+AGG + chr2.2:41997154-41997173 None:intergenic 40.0%
TTGCTACAATCTGAAGGTGA+AGG + chr2.2:41998432-41998451 None:intergenic 40.0%
! GAAACACCTCAAATGATGGA+GGG + chr2.2:42000668-42000687 None:intergenic 40.0%
! GAGATAATGGTGAAGAGAAG+AGG + chr2.2:42000497-42000516 None:intergenic 40.0%
! TTGTTTCCTTCCCTTGTTTC+TGG + chr2.2:41999710-41999729 None:intergenic 40.0%
!! AGATGGTTGATGGTTGTCAA+TGG - chr2.2:41998741-41998760 MS.gene025402:intron 40.0%
!! CTGGTGGAAGTTGATGATTA+TGG + chr2.2:42000371-42000390 None:intergenic 40.0%
!!! TTTGCTATTGGTTTTGCTGC+TGG + chr2.2:42000390-42000409 None:intergenic 40.0%
AACTGACGAGTAGGTAACAC+TGG + chr2.2:41998399-41998418 None:intergenic 45.0%
AGCCTACTATCGTTGCAGTA+TGG - chr2.2:41998808-41998827 MS.gene025402:intron 45.0%
ATTGTAGGGCATGTTGGAGA+TGG + chr2.2:42000552-42000571 None:intergenic 45.0%
CCAAAGAGATGGAAGACTCT+TGG - chr2.2:41999482-41999501 MS.gene025402:intron 45.0%
CCAAGAGTCTTCCATCTCTT+TGG + chr2.2:41999485-41999504 None:intergenic 45.0%
CCGCATCATTCTCAATAGAG+AGG + chr2.2:41999110-41999129 None:intergenic 45.0%
CCTACTATCGTTGCAGTATG+GGG - chr2.2:41998810-41998829 MS.gene025402:intron 45.0%
GCCTACTATCGTTGCAGTAT+GGG - chr2.2:41998809-41998828 MS.gene025402:intron 45.0%
GGAAACACCTCAAATGATGG+AGG + chr2.2:42000669-42000688 None:intergenic 45.0%
GGAAAGGTGGGAAATGAACT+AGG - chr2.2:41999537-41999556 MS.gene025402:intron 45.0%
GGTGAATTTCTATAGCCGCT+AGG + chr2.2:42000105-42000124 None:intergenic 45.0%
TCCATTTGTGAACGAGGACA+TGG + chr2.2:42000787-42000806 None:intergenic 45.0%
TGAGCTAAACTCACATGGAC+CGG - chr2.2:42000199-42000218 MS.gene025402:intron 45.0%
! AGGACCAATCATCTGAGGTT+GGG - chr2.2:41998550-41998569 MS.gene025402:intron 45.0%
! GAGAGCTAGAGTGTCCATAA+GGG - chr2.2:41998578-41998597 MS.gene025402:intron 45.0%
! TTGCTCCCTTTTCCATTCCA+AGG - chr2.2:42000636-42000655 MS.gene025402:intron 45.0%
!! ACAGGTGCAAAGGTGTTTCA+AGG - chr2.2:42000311-42000330 MS.gene025402:CDS 45.0%
!! ATGATGGAGGGTATTGACCT+TGG + chr2.2:42000656-42000675 None:intergenic 45.0%
!!! AAAAGTATAAATAAATTATA+AGG - chr2.2:42000012-42000031 MS.gene025402:CDS 5.0%
!!! TAATTATTTGTTTATTTTTT+GGG + chr2.2:41998110-41998129 None:intergenic 5.0%
!!! TTAATTATTTGTTTATTTTT+TGG + chr2.2:41998111-41998130 None:intergenic 5.0%
!!! TTTTTTGTAATATATATTTA+TGG - chr2.2:41999067-41999086 MS.gene025402:CDS 5.0%
CCCCATACTGCAACGATAGT+AGG + chr2.2:41998813-41998832 None:intergenic 50.0%
GATAGTAGGCTCGTGGACAA+GGG + chr2.2:41998799-41998818 None:intergenic 50.0%
GATGGGAAAATCATGCAGCC+AGG - chr2.2:41997438-41997457 MS.gene025402:CDS 50.0%
GCCATGTCCTCGTTCACAAA+TGG - chr2.2:42000783-42000802 MS.gene025402:CDS 50.0%
TGCACCCAACCTCAGATGAT+TGG + chr2.2:41998557-41998576 None:intergenic 50.0%
TTCATGCCCAGTTCGTAAGC+AGG - chr2.2:41998835-41998854 MS.gene025402:intron 50.0%
! AATCATCTGAGGTTGGGTGC+AGG - chr2.2:41998556-41998575 MS.gene025402:intron 50.0%
! AGGTCAATATGTGGGGTGTC+TGG - chr2.2:42000129-42000148 MS.gene025402:intron 50.0%
! GAGGACCAATCATCTGAGGT+TGG - chr2.2:41998549-41998568 MS.gene025402:intron 50.0%
! GGAGAGCTAGAGTGTCCATA+AGG - chr2.2:41998577-41998596 MS.gene025402:intron 50.0%
! GGTCAATATGTGGGGTGTCT+GGG - chr2.2:42000130-42000149 MS.gene025402:intron 50.0%
! GTCAATATGTGGGGTGTCTG+GGG - chr2.2:42000131-42000150 MS.gene025402:intron 50.0%
!! GGAGGGTATTGACCTTGGAA+TGG + chr2.2:42000651-42000670 None:intergenic 50.0%
!!! GCTATTGGTTTTGCTGCTGG+TGG + chr2.2:42000387-42000406 None:intergenic 50.0%
CGATAGTAGGCTCGTGGACA+AGG + chr2.2:41998800-41998819 None:intergenic 55.0%
CTGCAACGATAGTAGGCTCG+TGG + chr2.2:41998806-41998825 None:intergenic 55.0%
TCGTGGACAAGGGTTGCCCT+TGG + chr2.2:41998789-41998808 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 41996980 42000860 41996980 ID=MS.gene025402
chr2.2 mRNA 41996980 42000860 41996980 ID=MS.gene025402.t1;Parent=MS.gene025402
chr2.2 exon 42000762 42000860 42000762 ID=MS.gene025402.t1.exon1;Parent=MS.gene025402.t1
chr2.2 CDS 42000762 42000860 42000762 ID=cds.MS.gene025402.t1;Parent=MS.gene025402.t1
chr2.2 exon 42000301 42000384 42000301 ID=MS.gene025402.t1.exon2;Parent=MS.gene025402.t1
chr2.2 CDS 42000301 42000384 42000301 ID=cds.MS.gene025402.t1;Parent=MS.gene025402.t1
chr2.2 exon 41999915 42000085 41999915 ID=MS.gene025402.t1.exon3;Parent=MS.gene025402.t1
chr2.2 CDS 41999915 42000085 41999915 ID=cds.MS.gene025402.t1;Parent=MS.gene025402.t1
chr2.2 exon 41999220 41999471 41999220 ID=MS.gene025402.t1.exon4;Parent=MS.gene025402.t1
chr2.2 CDS 41999220 41999471 41999220 ID=cds.MS.gene025402.t1;Parent=MS.gene025402.t1
chr2.2 exon 41998984 41999097 41998984 ID=MS.gene025402.t1.exon5;Parent=MS.gene025402.t1
chr2.2 CDS 41998984 41999097 41998984 ID=cds.MS.gene025402.t1;Parent=MS.gene025402.t1
chr2.2 exon 41996980 41997525 41996980 ID=MS.gene025402.t1.exon6;Parent=MS.gene025402.t1
chr2.2 CDS 41996980 41997525 41996980 ID=cds.MS.gene025402.t1;Parent=MS.gene025402.t1
Gene Sequence

>MS.gene025402

ATGAAATTGCTGGAAGTTGATGAAAGGCCACCATATGATAATAACAATAAAGATCTACAAAACAAGATCGATGAATCCTCTTCCTTCCATAAGAATACTGTAAGTTTTCATTTATGCCATGTTTGTGTATACTGGATTAATTTCCATATTTGATATTTGATTATTAATTAGCCTCGATGATCTCGATTCATACATAAAATTTACAATTTTGTTCATTGCTTTATTTTCTAATGCTTTATAAAGAGTCATATACTTTGTTTAGGTAAATAGACTTGAGCATCTAATGCTTTATAAGTTGAAGTAGTTTTTTTTCTTTTTAAAGAAGTTGAAGTAGTTTTTGTTCTTTGTTAGCTTATGATGAAAATCACGGGTGTGGACAAAGATTTCATCTCATCATTCTTTTATCTGAATTACTTATTTTGTTAACAAAAAAACCTAACCTAATAACTTGACATTTTGATGGGAAAATCATGCAGCCAGGATCTACTCTTTTATTAAGTTTGAGCCTTAATAACACAACACAACTTCCCCATCAAATTCAAGGAAATCCCCAAATCCAAGTAAGTGATTAATCTTATTAGTGAATTTAAAGCATGTTTTTTTTAATTAACTTTCACACTTAAATTTTAATTTATTTATATGAGAGTATATAAAGTAATTAATCTTATTAGTGAATTAAGAGCATGTGTTATTTTTAATTAACTTTCACACATAAATTTAAATTTATTTATATAATAGTATAAAAGTAATTTTTTGATTTGATAATTGCTCTAAGATTGGCAATATACGAGTGAAGCTTGATGAAGCAAAGAAGGAGAATGAAAATTTAAGAGGTATGCTAAATCTGGTCAACGATCGATGCAATGTTCTTCAGAATCGTTTACTTTTGGCTATGCATATGCATCAATCATCTTCCTTGCCTCAAAATAATCACAACTTGCTGCTGGTAAGTTTGACTTCAAAATTTTGTTCACATATCCTTATTTGATTCACACTCATTTTTTTGTTCTATTAAATTTGCTTGAAATATTTAATGATCTCATGTATTTGGTCAACACGTCTTAATATTTTCAATAGTTATAGCTACAATTAATTAATTATATCCCGTCTAGTTACAAATAGATAAACCCAAAAAATAAACAAATAATTAAGATTATAAAAAATTAGAAACGTGTAATTTTTTGTTGTTTATGTAATTAACATGGCTTAGATGCTGTAGTGTTGTCGATCATCAATTGGTTGGACACATTCAATATGATAAATTAAACTTTAATTTTGGATTTGGTGAATGTGGTCAATTAAACTTTTTCAACCTATGATATTTTCAACTTGTAATAAATATAATTGCATATATTAACCTTTGTACATTTTTTCTTCATAATTAATTAGAAGGGAAATACACAAGATGCGGAGAAACCAGTGTTACCTACTCGTCAGTTCTTTGATGAGCCTTCACCTTCAGATTGTAGCAAAAATAATGGATTTGCCATAGTGGAAAACAATGAAAACAACATGGGAAGAAATCTTGCATGTGAATACATTAATGAAGGTGAAATAAATAGCAAAATAGAGGACCAATCATCTGAGGTTGGGTGCAGGAGAGCTAGAGTGTCCATAAGGGCACGATCAGATTTTGCATTTGTAAGGACATGCATGCAACTCTTCTTTTTTTATTAAGTACAATAATCTAATAATCTACTAATCAATTACTACTTATGATGATTGATCAGTATTTTAATTATTTATTAATTTGTAATGTTTAGATGGTTGATGGTTGTCAATGGAGAAAATATGGACAAAAGACAGCCAAGGGCAACCCTTGTCCACGAGCCTACTATCGTTGCAGTATGGGGACTTCATGCCCAGTTCGTAAGCAGGTAAACTATAAATTAATTTGTTAGATCATACCATTTTCATATGATATTACCTAAAAACAATCATAAGTTGTAGTTTTTTTTTCTTCTTATCTTACAAATCGATTTTATAGAAGCGAGTTGGCTTTTATGTAAAATATAAGAATTTCAATTGAAAATTGATAAATTTAAGCCCAACAAATTCTAAAAAAAATTATTTCAAAAATTGAATCTTTTTTTGTAATATATATTTATGGTATCACTTCACTTGCTTCCTCTCTATTGAGAATGATGCGGACAGACTATTTATTTGAGGGGAAAATTAAAGAAAAAATACGATGTTCTCAATATATTTTTGTTGTCAATTTTTTTTTCTTGTTTTTATATGTATAATAGAAATGAAGAAAGTACAATATTTTATATCTTGATATTTGAGCAAATATGTTGTTATGGGTTACTTTGTTCTAGTGTTCGTCCTTAATTGATGATGCACTACAATGAGACATCACAACAATTTTAATGCATTAAAAAAGGGGAGGAACATTGCAAGCTCTCTTCAAATTTAGGCTCTAATTACTTTCTAAAAACTTTTTCAGTTTTTATGTTTTAAAATTTTATGTTCATTTTAAAATGCTACCAAAGAATGCTACCAAAGAGATGGAAGACTCTTGGTTCCAACTAAATGGAGGTGAAAGTTTGTGAAAGGAAAGGTGGGAAATGAACTAGGAAGAACAAATTTTCAAAATTAATGAAAAAAAGATGTATTTAGCATTTTCAAATTTTTTGAACATGGGGTTTATCTGTTTTTGAATATATGAAAAAAGATGATGATGAAAGTATTCTTTTATGTTTAATGATTTAAGTTGAAATGAAACCAGAAACAAGGGAAGGAAACAATACCTTGTTTATTTGAGCTTTGATACCTACACATTCAACCTAAAAGAATGAAACTGAGAATTTCTATAGCTCCTATACAATAGTTAGTTCTGGAATTTTGCAACACTTAAAATAAACATTATTTTAACATAAATTTTCAATTATTTATTAAAATGACATTTTTCACTTATTTAACTTCTCATATACACTATATACTTTTGTTCAAAATAATGAAAACTTGTCTCATTTAAAATGAACAAAACTTACTATACATAGGTGTAATTGTAAGTGCACTTCTTCATAAAAAGTATAAATAAATTATAAGGATGACAATATTTTAAGGAACAAATTATTGTGGTGTAATTCTCTGTTTTTTTTTTTTTTTTCAAGTAGCCTAGCGGCTATAGAAATTCACCTTAAAGGTCAATATGTGGGGTGTCTGGGGTTCGAACCCCATCCCTGCATATAATATGCATTATCCCTTATCAAGTGAGCTAAACTCACATGGACCGGTGTAATTCTTTTTTAAAATGGTGTGTATATGGATGAAATTTTCTTGCATATTGTTAACTCAAAATATGATATTAATTACATTCCAATTAACAGGTGCAAAGGTGTTTCAAGGATGAAAGTGTGTTCATCACAACCTATGAAGGAAACCATAATCATCAACTTCCACCAGCAGCAAAACCAATAGCAAACTTAACATCATCAGCACTCAACACATTCCTTCCTACCTCATCAACAAATTTACAACAATATGGCAACAATCTAACAAACACATTCCTCTTCTCTTCACCATTATCTCCACCAAACTCAAATTCTATAGCTACTTTCTCTCCATCTCCAACATGCCCTACAATTACACTTGATTTTACACTCCCTCCAAGTAACTATTTGCAATTCAAAAATCATAAACAATCATCATTGCTCCCTTTTCCATTCCAAGGTCAATACCCTCCATCATTTGAGGTGTTTCCAAATATGATAAACAATGAGAGAAAACTTGCTTTGGTTGATGTTGTGAGTGAAGCTCTAGAAAAAGATCCTTCTCTCAAGGAAGCATTATTTTCAGCCATGTCCTCGTTCACAAATGGAGATTCTTCAAATATTAATAACCAAAGCCAATTACCTAGTAAGAGTAGTGGTTAA
Protein sequence

>MS.gene025402.t1

MKLLEVDERPPYDNNNKDLQNKIDESSSFHKNTPGSTLLLSLSLNNTTQLPHQIQGNPQIQIGNIRVKLDEAKKENENLRGMLNLVNDRCNVLQNRLLLAMHMHQSSSLPQNNHNLLLKGNTQDAEKPVLPTRQFFDEPSPSDCSKNNGFAIVENNENNMGRNLACEYINEGEINSKIEDQSSEVGCRRARVSIRARSDFAFMVDGCQWRKYGQKTAKGNPCPRAYYRCSMGTSCPVRKQVQRCFKDESVFITTYEGNHNHQLPPAAKPIANLTSSALNTFLPTSSTNLQQYGNNLTNTFLFSSPLSPPNSNSIATFSPSPTCPTITLDFTLPPSNYLQFKNHKQSSLLPFPFQGQYPPSFEVFPNMINNERKLALVDVVSEALEKDPSLKEALFSAMSSFTNGDSSNINNQSQLPSKSSG