Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene02558.t1 | XP_013461302.1 | 97.6 | 124 | 3 | 0 | 1 | 124 | 1 | 124 | 6.90E-54 | 221.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene02558.t1 | Q5Q995 | 39.5 | 119 | 64 | 2 | 4 | 114 | 5 | 123 | 1.1e-12 | 75.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene02558.t1 | I3T7J3 | 97.6 | 124 | 3 | 0 | 1 | 124 | 1 | 124 | 5.0e-54 | 221.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene02558.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene02558.t1 | MTR_3g088525 | 97.581 | 124 | 3 | 0 | 1 | 124 | 1 | 124 | 1.42e-78 | 238 |
| MS.gene02558.t1 | MTR_3g088515 | 65.761 | 184 | 19 | 1 | 121 | 260 | 7 | 190 | 7.63e-71 | 223 |
| MS.gene02558.t1 | MTR_1g046340 | 80.180 | 111 | 22 | 0 | 126 | 236 | 1 | 111 | 3.32e-57 | 190 |
| MS.gene02558.t1 | MTR_1g046320 | 71.560 | 109 | 31 | 0 | 126 | 234 | 1 | 109 | 1.84e-46 | 162 |
| MS.gene02558.t1 | MTR_1g046200 | 71.963 | 107 | 30 | 0 | 130 | 236 | 2 | 108 | 1.65e-45 | 159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene02558.t1 | AT1G77350 | 74.590 | 122 | 30 | 1 | 1 | 121 | 1 | 122 | 1.52e-55 | 179 |
| MS.gene02558.t1 | AT1G77350 | 74.590 | 122 | 30 | 1 | 1 | 121 | 1 | 122 | 1.52e-55 | 179 |
Find 83 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGCAGTGTTGGTCATAATT+TGG | 0.253758 | 3.2:+75431353 | None:intergenic |
| TGAGAAGCCATGGCGGGGTC+TGG | 0.324778 | 3.2:-75435693 | None:intergenic |
| CAGTTAGGAGCACAAGAAAT+AGG | 0.332990 | 3.2:+75435550 | None:intergenic |
| ACTTTCCCGGCGGTGGAAAC+GGG | 0.333345 | 3.2:-75428393 | MS.gene02558:CDS |
| AACTTTCCCGGCGGTGGAAA+CGG | 0.347641 | 3.2:-75428394 | MS.gene02558:CDS |
| CAGAGAAGAAAATTCATTAG+AGG | 0.353395 | 3.2:-75430943 | MS.gene02558:CDS |
| TGCCATATTGCGTGTCCGCC+TGG | 0.359320 | 3.2:+75428553 | None:intergenic |
| ATGAGCTTCACAAAAGCAAT+TGG | 0.382357 | 3.2:+75431086 | None:intergenic |
| GAGACAGCCGGTGCAAAATC+TGG | 0.384994 | 3.2:-75434875 | MS.gene02558:CDS |
| TGAATGCAGTTTCCTGTTCT+CGG | 0.393893 | 3.2:-75433698 | MS.gene02558:intron |
| CGCGTCAAAGCACATTCAAT+TGG | 0.401347 | 3.2:-75431002 | MS.gene02558:CDS |
| AGCTCAAACGGAGGCGGTGG+TGG | 0.412381 | 3.2:-75428517 | MS.gene02558:CDS |
| ACTTTCCTAGTGGTGGTAAA+GGG | 0.412711 | 3.2:-75428483 | MS.gene02558:CDS |
| GGCCAGGCGGACACGCAATA+TGG | 0.417457 | 3.2:-75428555 | MS.gene02558:CDS |
| GTCATAAACTTTCCCGGCGG+TGG | 0.417743 | 3.2:-75428400 | MS.gene02558:CDS |
| GCAAAATCTGGCTGGGGTGC+TGG | 0.425345 | 3.2:-75434863 | MS.gene02558:intron |
| TCGGAATTATTCACAATACT+TGG | 0.430748 | 3.2:-75435591 | MS.gene02558:CDS |
| GAGAAGCCATGGCGGGGTCT+GGG | 0.434467 | 3.2:-75435692 | None:intergenic |
| AACTTTCCTAGTGGTGGTAA+AGG | 0.439112 | 3.2:-75428484 | MS.gene02558:CDS |
| GTGTTGATGGCTATATAGTT+TGG | 0.439892 | 3.2:-75428576 | MS.gene02558:CDS |
| CTCGGCTGTGTTGCTCTATG+AGG | 0.447834 | 3.2:-75433680 | MS.gene02558:CDS |
| GTATGTGTTGTGTTGATTTG+TGG | 0.448016 | 3.2:+75429836 | None:intergenic |
| GCAAATGTCATAAACTTTCC+CGG | 0.460290 | 3.2:-75428406 | MS.gene02558:CDS |
| AGCTCCAGTGGAAGCTCAAA+CGG | 0.465910 | 3.2:-75428529 | MS.gene02558:CDS |
| GCCTCCGTTTGAGCTTCCAC+TGG | 0.472448 | 3.2:+75428525 | None:intergenic |
| TTGCTATGAGAAGCCATGGC+GGG | 0.473545 | 3.2:-75435699 | None:intergenic |
| AGAGCAACACAGCCGAGAAC+AGG | 0.475210 | 3.2:+75433686 | None:intergenic |
| AGCCGGTGCAAAATCTGGCT+GGG | 0.475968 | 3.2:-75434870 | MS.gene02558:CDS |
| CAGCCGGTGCAAAATCTGGC+TGG | 0.479798 | 3.2:-75434871 | MS.gene02558:CDS |
| ATGAAAGCAGAAAAGAAGAA+AGG | 0.488071 | 3.2:-75430621 | MS.gene02558:intron |
| CTCTTCCTCTATGATCCCTT+AGG | 0.492258 | 3.2:+75431030 | None:intergenic |
| TTGTGGCAATATGTGATGAT+AGG | 0.496982 | 3.2:+75429853 | None:intergenic |
| GATGACAATGAATAGAAGAA+AGG | 0.500365 | 3.2:+75435653 | None:intergenic |
| TCTCATAATGCTTCTCTATG+AGG | 0.504327 | 3.2:+75431302 | None:intergenic |
| TATGAGGTTAACAAGCTCTC+TGG | 0.505681 | 3.2:-75433664 | MS.gene02558:CDS |
| GGGATCATAGAGGAAGAGAT+TGG | 0.510517 | 3.2:-75431025 | MS.gene02558:CDS |
| CTTTCCCGGCGGTGGAAACG+GGG | 0.511813 | 3.2:-75428392 | MS.gene02558:CDS |
| GGAAGCTCAAACGGAGGCGG+TGG | 0.512147 | 3.2:-75428520 | MS.gene02558:CDS |
| ATCATAAACTTTCCTAGTGG+TGG | 0.514983 | 3.2:-75428490 | MS.gene02558:CDS |
| AAGCACATTCAATTGGTGGC+AGG | 0.516729 | 3.2:-75430995 | MS.gene02558:CDS |
| AAGAAGAAAGGAGTTGACAA+GGG | 0.522584 | 3.2:-75430609 | MS.gene02558:CDS |
| GAGGGACATAAGTTAAGCGT+TGG | 0.524422 | 3.2:-75428349 | MS.gene02558:CDS |
| TAGAAGAAAGGAATACATCA+TGG | 0.524516 | 3.2:+75435665 | None:intergenic |
| AGAGAAGCATTATGAGAGAA+TGG | 0.527328 | 3.2:-75431296 | MS.gene02558:intron |
| GATGGCTATATAGTTTGGCC+AGG | 0.530422 | 3.2:-75428571 | MS.gene02558:CDS |
| GAATTATTCACAATACTTGG+AGG | 0.534232 | 3.2:-75435588 | MS.gene02558:CDS |
| AATTATTCACAATACTTGGA+GGG | 0.538766 | 3.2:-75435587 | MS.gene02558:CDS |
| AAAGAAGAAAGGAGTTGACA+AGG | 0.542225 | 3.2:-75430610 | MS.gene02558:CDS |
| ACCATGTAGTGATGCATACT+CGG | 0.544399 | 3.2:+75434558 | None:intergenic |
| TGAGCTTCACAAAAGCAATT+GGG | 0.545679 | 3.2:+75431087 | None:intergenic |
| GTCAAAGCACATTCAATTGG+TGG | 0.546694 | 3.2:-75430999 | MS.gene02558:CDS |
| TCCAGTGGAAGCTCAAACGG+AGG | 0.548477 | 3.2:-75428526 | MS.gene02558:CDS |
| TTTCAATATGTGTGAAAACA+AGG | 0.554136 | 3.2:+75430910 | None:intergenic |
| GAGGAACACCTTGGCAGTGT+TGG | 0.557953 | 3.2:+75431342 | None:intergenic |
| GCCGGTGCAAAATCTGGCTG+GGG | 0.558097 | 3.2:-75434869 | MS.gene02558:CDS |
| ATTTGTAGTTGGTGAGACAC+AGG | 0.559962 | 3.2:-75428617 | MS.gene02558:intron |
| AGCTCATGCTTTCGAAACAA+AGG | 0.561664 | 3.2:-75431070 | MS.gene02558:CDS |
| GGAAGACTTTGAAGAAGTTG+AGG | 0.564880 | 3.2:+75431323 | None:intergenic |
| CCGTGGGAAACTTGCATCGT+CGG | 0.565118 | 3.2:-75435610 | MS.gene02558:CDS |
| GCTCATGCTTTCGAAACAAA+GGG | 0.566004 | 3.2:-75431069 | MS.gene02558:CDS |
| TGCTATGAGAAGCCATGGCG+GGG | 0.568516 | 3.2:-75435698 | None:intergenic |
| AGTGGAAGCTCAAACGGAGG+CGG | 0.571893 | 3.2:-75428523 | MS.gene02558:CDS |
| TTTCCTAGTGGTGGTAAAGG+GGG | 0.582225 | 3.2:-75428481 | MS.gene02558:CDS |
| CACTTCAAGAAATGTACCGT+GGG | 0.587600 | 3.2:-75435626 | MS.gene02558:CDS |
| CTTTCCTAGTGGTGGTAAAG+GGG | 0.587943 | 3.2:-75428482 | MS.gene02558:CDS |
| GGCTATATAGTTTGGCCAGG+CGG | 0.594243 | 3.2:-75428568 | MS.gene02558:CDS |
| GAAGAAGTTGAGGAACACCT+TGG | 0.598409 | 3.2:+75431333 | None:intergenic |
| ACCGAGTATGCATCACTACA+TGG | 0.601750 | 3.2:-75434559 | MS.gene02558:intron |
| TTTCCCGGCGGTGGAAACGG+GGG | 0.606258 | 3.2:-75428391 | MS.gene02558:CDS |
| TTTATCCTAAGGGATCATAG+AGG | 0.616153 | 3.2:-75431035 | MS.gene02558:CDS |
| GCAATATGGCAAAGCTCCAG+TGG | 0.616159 | 3.2:-75428541 | MS.gene02558:CDS |
| GGAACTCCCAGACCCCGCCA+TGG | 0.620572 | 3.2:+75435686 | None:intergenic |
| AATTATGACCAACACTGCCA+AGG | 0.625272 | 3.2:-75431350 | MS.gene02558:CDS |
| GGTACATTTCTTGAAGTGAG+AGG | 0.637005 | 3.2:+75435631 | None:intergenic |
| CCCGGAAGTGGAAAACGGGG+TGG | 0.655848 | 3.2:-75428433 | MS.gene02558:CDS |
| ACAACACATACCGAAAAGAG+AGG | 0.656927 | 3.2:-75429824 | MS.gene02558:CDS |
| CCGACGATGCAAGTTTCCCA+CGG | 0.661665 | 3.2:+75435610 | None:intergenic |
| GGAGTCACTTGTTGAAACAG+CGG | 0.662603 | 3.2:+75433639 | None:intergenic |
| ATTATCATAAACTTTCCTAG+TGG | 0.664481 | 3.2:-75428493 | MS.gene02558:CDS |
| TCACTTCAAGAAATGTACCG+TGG | 0.674064 | 3.2:-75435627 | MS.gene02558:CDS |
| TTTCCCGGAAGTGGAAAACG+GGG | 0.695053 | 3.2:-75428436 | MS.gene02558:CDS |
| ATGTAGTGATGCATACTCGG+TGG | 0.725698 | 3.2:+75434561 | None:intergenic |
| AATGTCATAAACTTTCCCGG+CGG | 0.795660 | 3.2:-75428403 | MS.gene02558:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 75428327 | 75435706 | 75428327 | ID=MS.gene02558 |
| chr3.2 | mRNA | 75428327 | 75435706 | 75428327 | ID=MS.gene02558.t1;Parent=MS.gene02558 |
| chr3.2 | exon | 75435551 | 75435706 | 75435551 | ID=MS.gene02558.t1.exon1;Parent=MS.gene02558.t1 |
| chr3.2 | CDS | 75435551 | 75435706 | 75435551 | ID=cds.MS.gene02558.t1;Parent=MS.gene02558.t1 |
| chr3.2 | exon | 75434864 | 75434915 | 75434864 | ID=MS.gene02558.t1.exon2;Parent=MS.gene02558.t1 |
| chr3.2 | CDS | 75434864 | 75434915 | 75434864 | ID=cds.MS.gene02558.t1;Parent=MS.gene02558.t1 |
| chr3.2 | exon | 75434560 | 75434626 | 75434560 | ID=MS.gene02558.t1.exon3;Parent=MS.gene02558.t1 |
| chr3.2 | CDS | 75434560 | 75434626 | 75434560 | ID=cds.MS.gene02558.t1;Parent=MS.gene02558.t1 |
| chr3.2 | exon | 75433623 | 75433711 | 75433623 | ID=MS.gene02558.t1.exon4;Parent=MS.gene02558.t1 |
| chr3.2 | CDS | 75433623 | 75433711 | 75433623 | ID=cds.MS.gene02558.t1;Parent=MS.gene02558.t1 |
| chr3.2 | exon | 75431297 | 75431379 | 75431297 | ID=MS.gene02558.t1.exon5;Parent=MS.gene02558.t1 |
| chr3.2 | CDS | 75431297 | 75431379 | 75431297 | ID=cds.MS.gene02558.t1;Parent=MS.gene02558.t1 |
| chr3.2 | exon | 75430854 | 75431114 | 75430854 | ID=MS.gene02558.t1.exon6;Parent=MS.gene02558.t1 |
| chr3.2 | CDS | 75430854 | 75431114 | 75430854 | ID=cds.MS.gene02558.t1;Parent=MS.gene02558.t1 |
| chr3.2 | exon | 75430562 | 75430633 | 75430562 | ID=MS.gene02558.t1.exon7;Parent=MS.gene02558.t1 |
| chr3.2 | CDS | 75430562 | 75430633 | 75430562 | ID=cds.MS.gene02558.t1;Parent=MS.gene02558.t1 |
| chr3.2 | exon | 75429816 | 75429900 | 75429816 | ID=MS.gene02558.t1.exon8;Parent=MS.gene02558.t1 |
| chr3.2 | CDS | 75429816 | 75429900 | 75429816 | ID=cds.MS.gene02558.t1;Parent=MS.gene02558.t1 |
| chr3.2 | exon | 75428327 | 75428631 | 75428327 | ID=MS.gene02558.t1.exon9;Parent=MS.gene02558.t1 |
| chr3.2 | CDS | 75428327 | 75428631 | 75428327 | ID=cds.MS.gene02558.t1;Parent=MS.gene02558.t1 |
| Gene Sequence |
| Protein sequence |
