Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025630.t1 | XP_013450172.1 | 91.1 | 270 | 23 | 1 | 1 | 269 | 1 | 270 | 2.00E-103 | 385.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025630.t1 | Q9FMC8 | 39.4 | 226 | 97 | 10 | 1 | 199 | 1 | 213 | 1.2e-27 | 125.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025630.t1 | G7ZUW4 | 91.1 | 270 | 23 | 1 | 1 | 269 | 1 | 270 | 1.5e-103 | 385.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene025630.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025630.t1 | MTR_7g105780 | 97.561 | 287 | 6 | 1 | 1 | 286 | 1 | 287 | 0.0 | 558 |
| MS.gene025630.t1 | MTR_1g111640 | 49.282 | 209 | 83 | 7 | 1 | 196 | 1 | 199 | 6.48e-56 | 181 |
| MS.gene025630.t1 | MTR_1g058000 | 41.935 | 217 | 100 | 7 | 31 | 239 | 23 | 221 | 2.40e-40 | 140 |
| MS.gene025630.t1 | MTR_7g095150 | 33.766 | 154 | 78 | 5 | 65 | 198 | 51 | 200 | 4.94e-17 | 78.2 |
| MS.gene025630.t1 | MTR_1g072110 | 38.168 | 131 | 49 | 5 | 96 | 198 | 108 | 234 | 2.05e-16 | 77.0 |
| MS.gene025630.t1 | MTR_1g103640 | 38.261 | 115 | 50 | 6 | 97 | 198 | 73 | 179 | 1.23e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025630.t1 | AT5G04820 | 40.079 | 252 | 111 | 13 | 1 | 222 | 1 | 242 | 2.29e-40 | 141 |
| MS.gene025630.t1 | AT2G36050 | 40.805 | 174 | 72 | 7 | 31 | 200 | 29 | 175 | 6.06e-25 | 100 |
| MS.gene025630.t1 | AT3G52540 | 40.306 | 196 | 100 | 8 | 86 | 270 | 91 | 280 | 1.11e-21 | 92.4 |
| MS.gene025630.t1 | AT1G05420 | 37.963 | 108 | 61 | 3 | 97 | 198 | 127 | 234 | 2.12e-11 | 63.2 |
| MS.gene025630.t1 | AT1G05420 | 37.963 | 108 | 61 | 3 | 97 | 198 | 111 | 218 | 2.12e-11 | 62.8 |
Find 35 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCAAGTAAACTGAGTTAA+TGG | 0.182052 | 7.3:+8667717 | None:intergenic |
| CTTTGTTGTTAGAACAAGTT+AGG | 0.274105 | 7.3:-8667111 | MS.gene025630:CDS |
| TTCGATGGAATCAACTTGTT+TGG | 0.312302 | 7.3:+8667621 | None:intergenic |
| AATAATAAAGCTGCTGCAAT+AGG | 0.359001 | 7.3:-8667526 | MS.gene025630:CDS |
| CACTCGTTGTGTTTCTTGTT+CGG | 0.365942 | 7.3:-8667170 | MS.gene025630:CDS |
| GACTTTCGAAAATCGATATA+AGG | 0.369168 | 7.3:+8667439 | None:intergenic |
| TGAGACCAACTCCATCTTAG+AGG | 0.373515 | 7.3:-8667551 | MS.gene025630:CDS |
| AACCATTAACTCAGTTTACT+TGG | 0.384415 | 7.3:-8667719 | MS.gene025630:CDS |
| AAGATGGAGTTGGTCTCATC+TGG | 0.396490 | 7.3:+8667556 | None:intergenic |
| ATCAATCATGGCTATATTGT+TGG | 0.406010 | 7.3:-8667328 | MS.gene025630:CDS |
| GAAAAGCTAGAATCACAGTT+TGG | 0.407128 | 7.3:+8667667 | None:intergenic |
| GAAGAACTATAAAACGATAA+AGG | 0.409329 | 7.3:+8667211 | None:intergenic |
| AAGCTCATGATGTTAAAGAT+TGG | 0.417820 | 7.3:-8667399 | MS.gene025630:CDS |
| TTTATTATTAACCTCTAAGA+TGG | 0.418526 | 7.3:+8667540 | None:intergenic |
| AGCTCATGATGTTAAAGATT+GGG | 0.430684 | 7.3:-8667398 | MS.gene025630:CDS |
| TTCGGAAATTCGCGAGGATG+AGG | 0.438055 | 7.3:-8667152 | MS.gene025630:CDS |
| AATATTCATCTTCTTCATCA+TGG | 0.472237 | 7.3:-8667816 | MS.gene025630:CDS |
| TTTGTTGTTAGAACAAGTTA+GGG | 0.507023 | 7.3:-8667110 | MS.gene025630:CDS |
| TCCATCGAAACTGTGATTCG+AGG | 0.507125 | 7.3:-8667607 | MS.gene025630:CDS |
| TAAAACGATAAAGGAGAAGA+AGG | 0.515224 | 7.3:+8667220 | None:intergenic |
| GTCAATGAGAAAATCAATCA+TGG | 0.520445 | 7.3:-8667340 | MS.gene025630:CDS |
| AACACAACGAGTGCTATAAG+AGG | 0.520738 | 7.3:+8667180 | None:intergenic |
| TTCGAGGATTATCTTCTGAT+CGG | 0.524502 | 7.3:-8667591 | MS.gene025630:CDS |
| TCCTCGAATCACAGTTTCGA+TGG | 0.529971 | 7.3:+8667606 | None:intergenic |
| ATTAACCTCTAAGATGGAGT+TGG | 0.540469 | 7.3:+8667546 | None:intergenic |
| AATAAAGCTGCTGCAATAGG+AGG | 0.582511 | 7.3:-8667523 | MS.gene025630:CDS |
| GGTTGGTGACAATATGGCCA+TGG | 0.583924 | 7.3:+8667793 | None:intergenic |
| CATCTTCTTCATCATGGCCA+TGG | 0.594079 | 7.3:-8667810 | MS.gene025630:CDS |
| AAAGCTGCTGCAATAGGAGG+TGG | 0.598773 | 7.3:-8667520 | MS.gene025630:CDS |
| TCTAACAACAAAGAAGAACT+AGG | 0.600863 | 7.3:+8667121 | None:intergenic |
| GTTGTTAGAACAAGTTAGGG+AGG | 0.604085 | 7.3:-8667107 | MS.gene025630:CDS |
| AAAACTACACTGTCTTTGAA+CGG | 0.622124 | 7.3:+8667481 | None:intergenic |
| ATCACAGTTTGGAGAAACAG+TGG | 0.633886 | 7.3:+8667678 | None:intergenic |
| CATGATGTTAAAGATTGGGA+AGG | 0.635146 | 7.3:-8667394 | MS.gene025630:CDS |
| TTCTTGTTCGGAAATTCGCG+AGG | 0.660121 | 7.3:-8667158 | MS.gene025630:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATTATTAACCTCTAAGA+TGG | + | chr7.3:8667367-8667386 | None:intergenic | 20.0% |
| ! | AATATTCATCTTCTTCATCA+TGG | - | chr7.3:8667088-8667107 | MS.gene025630:CDS | 25.0% |
| ! | GAAGAACTATAAAACGATAA+AGG | + | chr7.3:8667696-8667715 | None:intergenic | 25.0% |
| !!! | TTTGTTGTTAGAACAAGTTA+GGG | - | chr7.3:8667794-8667813 | MS.gene025630:CDS | 25.0% |
| AAAACTACACTGTCTTTGAA+CGG | + | chr7.3:8667426-8667445 | None:intergenic | 30.0% | |
| AACCATTAACTCAGTTTACT+TGG | - | chr7.3:8667185-8667204 | MS.gene025630:CDS | 30.0% | |
| AAGCTCATGATGTTAAAGAT+TGG | - | chr7.3:8667505-8667524 | MS.gene025630:CDS | 30.0% | |
| AATAATAAAGCTGCTGCAAT+AGG | - | chr7.3:8667378-8667397 | MS.gene025630:CDS | 30.0% | |
| AGCTCATGATGTTAAAGATT+GGG | - | chr7.3:8667506-8667525 | MS.gene025630:CDS | 30.0% | |
| ATCAATCATGGCTATATTGT+TGG | - | chr7.3:8667576-8667595 | MS.gene025630:CDS | 30.0% | |
| GACTTTCGAAAATCGATATA+AGG | + | chr7.3:8667468-8667487 | None:intergenic | 30.0% | |
| GTCAATGAGAAAATCAATCA+TGG | - | chr7.3:8667564-8667583 | MS.gene025630:CDS | 30.0% | |
| TAAAACGATAAAGGAGAAGA+AGG | + | chr7.3:8667687-8667706 | None:intergenic | 30.0% | |
| TCTAACAACAAAGAAGAACT+AGG | + | chr7.3:8667786-8667805 | None:intergenic | 30.0% | |
| TTCCAAGTAAACTGAGTTAA+TGG | + | chr7.3:8667190-8667209 | None:intergenic | 30.0% | |
| !! | CTTTGTTGTTAGAACAAGTT+AGG | - | chr7.3:8667793-8667812 | MS.gene025630:CDS | 30.0% |
| !!! | AGACAGTGTAGTTTTATCTA+TGG | - | chr7.3:8667431-8667450 | MS.gene025630:CDS | 30.0% |
| !!! | GCTTTTGTTGATTTATTGGT+TGG | - | chr7.3:8667600-8667619 | MS.gene025630:CDS | 30.0% |
| !!! | TAAAAGAGAGTGTTTTTGGT+TGG | + | chr7.3:8667131-8667150 | None:intergenic | 30.0% |
| !!! | TATCGATTTTCGAAAGTCTA+TGG | - | chr7.3:8667470-8667489 | MS.gene025630:CDS | 30.0% |
| !!! | TGGTGCTTTTGTTGATTTAT+TGG | - | chr7.3:8667596-8667615 | MS.gene025630:CDS | 30.0% |
| CATGATGTTAAAGATTGGGA+AGG | - | chr7.3:8667510-8667529 | MS.gene025630:CDS | 35.0% | |
| GAAAAGCTAGAATCACAGTT+TGG | + | chr7.3:8667240-8667259 | None:intergenic | 35.0% | |
| TTCGAGGATTATCTTCTGAT+CGG | - | chr7.3:8667313-8667332 | MS.gene025630:CDS | 35.0% | |
| ! | TTCGATGGAATCAACTTGTT+TGG | + | chr7.3:8667286-8667305 | None:intergenic | 35.0% |
| !! | ATTAACCTCTAAGATGGAGT+TGG | + | chr7.3:8667361-8667380 | None:intergenic | 35.0% |
| !!! | GCTCTAAAAGAGAGTGTTTT+TGG | + | chr7.3:8667135-8667154 | None:intergenic | 35.0% |
| !!! | GCTTTTGAGTTGGTATTTGA+AGG | - | chr7.3:8667542-8667561 | MS.gene025630:CDS | 35.0% |
| !!! | GTTTTTGGTTGGTGACAATA+TGG | + | chr7.3:8667120-8667139 | None:intergenic | 35.0% |
| AACACAACGAGTGCTATAAG+AGG | + | chr7.3:8667727-8667746 | None:intergenic | 40.0% | |
| AATAAAGCTGCTGCAATAGG+AGG | - | chr7.3:8667381-8667400 | MS.gene025630:CDS | 40.0% | |
| ATCACAGTTTGGAGAAACAG+TGG | + | chr7.3:8667229-8667248 | None:intergenic | 40.0% | |
| CACTCGTTGTGTTTCTTGTT+CGG | - | chr7.3:8667734-8667753 | MS.gene025630:CDS | 40.0% | |
| GTTGTTAGAACAAGTTAGGG+AGG | - | chr7.3:8667797-8667816 | MS.gene025630:CDS | 40.0% | |
| ! | CGATTTTCGAAAGTCTATGG+AGG | - | chr7.3:8667473-8667492 | MS.gene025630:CDS | 40.0% |
| !! | GTCTTCAAGAGCTTTTGAGT+TGG | - | chr7.3:8667532-8667551 | MS.gene025630:CDS | 40.0% |
| CATCTTCTTCATCATGGCCA+TGG | - | chr7.3:8667094-8667113 | MS.gene025630:CDS | 45.0% | |
| TCCATCGAAACTGTGATTCG+AGG | - | chr7.3:8667297-8667316 | MS.gene025630:CDS | 45.0% | |
| TCCTCGAATCACAGTTTCGA+TGG | + | chr7.3:8667301-8667320 | None:intergenic | 45.0% | |
| TGAGACCAACTCCATCTTAG+AGG | - | chr7.3:8667353-8667372 | MS.gene025630:CDS | 45.0% | |
| TTCTTGTTCGGAAATTCGCG+AGG | - | chr7.3:8667746-8667765 | MS.gene025630:CDS | 45.0% | |
| ! | AAGATGGAGTTGGTCTCATC+TGG | + | chr7.3:8667351-8667370 | None:intergenic | 45.0% |
| AAAGCTGCTGCAATAGGAGG+TGG | - | chr7.3:8667384-8667403 | MS.gene025630:CDS | 50.0% | |
| TTCGGAAATTCGCGAGGATG+AGG | - | chr7.3:8667752-8667771 | MS.gene025630:CDS | 50.0% | |
| !! | GGTTGGTGACAATATGGCCA+TGG | + | chr7.3:8667114-8667133 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 8667033 | 8667893 | 8667033 | ID=MS.gene025630 |
| chr7.3 | mRNA | 8667033 | 8667893 | 8667033 | ID=MS.gene025630.t1;Parent=MS.gene025630 |
| chr7.3 | exon | 8667033 | 8667893 | 8667033 | ID=MS.gene025630.t1.exon1;Parent=MS.gene025630.t1 |
| chr7.3 | CDS | 8667033 | 8667893 | 8667033 | ID=cds.MS.gene025630.t1;Parent=MS.gene025630.t1 |
| Gene Sequence |
| Protein sequence |
