Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025631.t1 | XP_013450173.1 | 99 | 199 | 2 | 0 | 1 | 199 | 1 | 199 | 1.10E-98 | 369.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025631.t1 | Q3EAL1 | 40.4 | 203 | 69 | 5 | 1 | 199 | 1 | 155 | 1.1e-24 | 114.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025631.t1 | G7ZUW3 | 99.0 | 199 | 2 | 0 | 1 | 199 | 1 | 199 | 7.6e-99 | 369.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene025631.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025631.t1 | MTR_7g105790 | 98.995 | 199 | 2 | 0 | 1 | 199 | 1 | 199 | 5.02e-149 | 411 |
| MS.gene025631.t1 | MTR_0907s0020 | 56.771 | 192 | 68 | 3 | 1 | 188 | 1 | 181 | 2.43e-64 | 197 |
| MS.gene025631.t1 | MTR_4g052040 | 37.234 | 188 | 98 | 5 | 1 | 180 | 1 | 176 | 8.65e-29 | 106 |
| MS.gene025631.t1 | MTR_4g052060 | 35.227 | 176 | 89 | 3 | 1 | 172 | 1 | 155 | 2.04e-24 | 94.7 |
| MS.gene025631.t1 | MTR_5g079710 | 36.875 | 160 | 74 | 4 | 18 | 174 | 20 | 155 | 2.54e-24 | 96.7 |
| MS.gene025631.t1 | MTR_8g103520 | 47.297 | 74 | 39 | 0 | 101 | 174 | 215 | 288 | 1.93e-17 | 78.6 |
| MS.gene025631.t1 | MTR_5g075210 | 47.826 | 69 | 36 | 0 | 110 | 178 | 280 | 348 | 1.67e-16 | 76.6 |
| MS.gene025631.t1 | MTR_4g028930 | 42.029 | 69 | 40 | 0 | 103 | 171 | 307 | 375 | 3.02e-14 | 70.5 |
| MS.gene025631.t1 | MTR_7g072875 | 40.000 | 70 | 42 | 0 | 102 | 171 | 211 | 280 | 5.95e-14 | 68.9 |
| MS.gene025631.t1 | MTR_7g072630 | 40.000 | 70 | 42 | 0 | 102 | 171 | 273 | 342 | 8.99e-14 | 68.9 |
| MS.gene025631.t1 | MTR_3g111780 | 52.778 | 72 | 34 | 0 | 110 | 181 | 198 | 269 | 2.16e-12 | 64.7 |
| MS.gene025631.t1 | MTR_8g087530 | 42.647 | 68 | 39 | 0 | 108 | 175 | 256 | 323 | 2.47e-12 | 64.7 |
| MS.gene025631.t1 | MTR_2g018030 | 42.857 | 63 | 36 | 0 | 113 | 175 | 280 | 342 | 3.86e-12 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025631.t1 | AT2G36026 | 47.024 | 168 | 60 | 4 | 8 | 174 | 11 | 150 | 6.08e-35 | 122 |
| MS.gene025631.t1 | AT3G52525 | 78.125 | 64 | 14 | 0 | 108 | 171 | 64 | 127 | 7.76e-33 | 115 |
| MS.gene025631.t1 | AT2G18500 | 56.923 | 65 | 28 | 0 | 110 | 174 | 226 | 290 | 1.15e-20 | 87.8 |
| MS.gene025631.t1 | AT5G19650 | 52.381 | 63 | 30 | 0 | 109 | 171 | 153 | 215 | 4.67e-18 | 79.3 |
| MS.gene025631.t1 | AT5G58360 | 38.462 | 78 | 46 | 1 | 96 | 171 | 208 | 285 | 8.19e-14 | 68.9 |
| MS.gene025631.t1 | AT2G30400 | 48.387 | 62 | 32 | 0 | 110 | 171 | 252 | 313 | 1.40e-13 | 68.2 |
| MS.gene025631.t1 | AT1G06920 | 43.077 | 65 | 37 | 0 | 110 | 174 | 243 | 307 | 1.52e-13 | 68.2 |
| MS.gene025631.t1 | AT5G01840 | 44.118 | 68 | 38 | 0 | 114 | 181 | 202 | 269 | 1.28e-12 | 65.1 |
| MS.gene025631.t1 | AT4G18830 | 44.118 | 68 | 38 | 0 | 105 | 172 | 277 | 344 | 3.04e-12 | 64.7 |
| MS.gene025631.t1 | AT5G22240 | 44.444 | 63 | 35 | 0 | 110 | 172 | 96 | 158 | 6.70e-12 | 62.0 |
Find 45 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATCCTTATCTTGATTTC+AGG | 0.232091 | 7.3:+8653097 | MS.gene025631:CDS |
| TAAGTGGGGTTCTTGGATTT+TGG | 0.239783 | 7.3:-8652844 | None:intergenic |
| TTCCATGCTTCAAATGATAT+TGG | 0.263152 | 7.3:+8653122 | MS.gene025631:CDS |
| CAAAGGAGAGTAGGTGGTTT+AGG | 0.273409 | 7.3:+8653033 | MS.gene025631:CDS |
| TGTTTGTGGTTGTAGTGTTT+GGG | 0.298136 | 7.3:-8652945 | None:intergenic |
| GTGTTTGTGGTTGTAGTGTT+TGG | 0.310441 | 7.3:-8652946 | None:intergenic |
| AACAGTTCAAAAGCTCTCTA+AGG | 0.371057 | 7.3:-8653170 | None:intergenic |
| CTAAACCACCTACTCTCCTT+TGG | 0.387710 | 7.3:-8653032 | None:intergenic |
| AAATGTGGTGGCAGTGTTTG+TGG | 0.395839 | 7.3:-8652959 | None:intergenic |
| GTTAATGTTGACGTGGCTTA+CGG | 0.407041 | 7.3:-8653320 | None:intergenic |
| GGTACGAGATGTATGCAAAC+TGG | 0.419878 | 7.3:-8653286 | None:intergenic |
| GTGGCTTACGGGTACGGGTA+CGG | 0.421387 | 7.3:-8653308 | None:intergenic |
| TTAATGTTGACGTGGCTTAC+GGG | 0.438584 | 7.3:-8653319 | None:intergenic |
| GTAGGTGGTTTAGGAAGAGC+TGG | 0.439315 | 7.3:+8653042 | MS.gene025631:CDS |
| TTAGGAAGAGCTGGAGGAGA+AGG | 0.440207 | 7.3:+8653051 | MS.gene025631:CDS |
| GTTGACGTGGCTTACGGGTA+CGG | 0.444382 | 7.3:-8653314 | None:intergenic |
| TGGCTTACGGGTACGGGTAC+GGG | 0.455892 | 7.3:-8653307 | None:intergenic |
| GTATGCAAACTGGGTGAACT+AGG | 0.462797 | 7.3:-8653276 | None:intergenic |
| ACAATAACACCATGATGATA+AGG | 0.476139 | 7.3:-8653210 | None:intergenic |
| AATAACCAGTACTCTTCATC+TGG | 0.479074 | 7.3:+8652886 | MS.gene025631:CDS |
| GGAATGGAGTTTCAACTATG+AGG | 0.482299 | 7.3:+8653253 | MS.gene025631:CDS |
| TTGACGTGGCTTACGGGTAC+GGG | 0.503790 | 7.3:-8653313 | None:intergenic |
| TGGAGGAGAAGGTGTGGCGG+TGG | 0.505343 | 7.3:+8653062 | MS.gene025631:CDS |
| CTCCAATATCATTTGAAGCA+TGG | 0.514713 | 7.3:-8653124 | None:intergenic |
| AGTACTCTTCATCTGGTCCT+TGG | 0.522312 | 7.3:+8652893 | MS.gene025631:CDS |
| CTGAATGCACCTTATCATCA+TGG | 0.534801 | 7.3:+8653201 | MS.gene025631:CDS |
| TCATATTTGTCTTCAGTCCA+AGG | 0.540198 | 7.3:-8652910 | None:intergenic |
| CTTTATAGTTAATGTTGACG+TGG | 0.566022 | 7.3:-8653327 | None:intergenic |
| AAGGACCAGATGAAGAGTAC+TGG | 0.567259 | 7.3:-8652891 | None:intergenic |
| GAATGCCTGAAATCAAGATA+AGG | 0.568676 | 7.3:-8653102 | None:intergenic |
| TGATTCAGATGCTTATGCAA+AGG | 0.569548 | 7.3:+8653008 | MS.gene025631:CDS |
| AACACTGTGTCGGTGAAACT+AGG | 0.582378 | 7.3:+8652772 | MS.gene025631:CDS |
| GTTTGTGGTTGTAGTGTTTG+GGG | 0.590441 | 7.3:-8652944 | None:intergenic |
| GCAAAGGACCAAAGGAGAGT+AGG | 0.596139 | 7.3:+8653024 | MS.gene025631:CDS |
| ATGCTTATGCAAAGGACCAA+AGG | 0.599434 | 7.3:+8653016 | MS.gene025631:CDS |
| GTACGAGATGTATGCAAACT+GGG | 0.606095 | 7.3:-8653285 | None:intergenic |
| TAGGTTGTGGAAGCTGCAGA+AGG | 0.607735 | 7.3:+8652791 | MS.gene025631:CDS |
| AAGAGCTGGAGGAGAAGGTG+TGG | 0.611252 | 7.3:+8653056 | MS.gene025631:CDS |
| GGTGGTTTAGGAAGAGCTGG+AGG | 0.612792 | 7.3:+8653045 | MS.gene025631:CDS |
| GTGTCGGTGAAACTAGGTTG+TGG | 0.621800 | 7.3:+8652778 | MS.gene025631:CDS |
| GCTTCTTCTGAACACTGTGT+CGG | 0.624101 | 7.3:+8652762 | MS.gene025631:CDS |
| GGAATAGTATGGTGAAAATG+TGG | 0.635363 | 7.3:-8652974 | None:intergenic |
| AGCTGGAGGAGAAGGTGTGG+CGG | 0.646351 | 7.3:+8653059 | MS.gene025631:CDS |
| AAGGACCAAAGGAGAGTAGG+TGG | 0.647195 | 7.3:+8653027 | MS.gene025631:CDS |
| ATAGTATGGTGAAAATGTGG+TGG | 0.720920 | 7.3:-8652971 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTGTAAAGTTTGTGTTTT+TGG | - | chr7.3:8652870-8652889 | None:intergenic | 20.0% |
| ! | ATCTGAATCAGAAAAATTTG+AGG | - | chr7.3:8652998-8653017 | None:intergenic | 25.0% |
| ! | GAAAAATTTGAGGAATAGTA+TGG | - | chr7.3:8652988-8653007 | None:intergenic | 25.0% |
| AAGATCCTTATCTTGATTTC+AGG | + | chr7.3:8653097-8653116 | MS.gene025631:CDS | 30.0% | |
| ACAATAACACCATGATGATA+AGG | - | chr7.3:8653213-8653232 | None:intergenic | 30.0% | |
| TTCCATGCTTCAAATGATAT+TGG | + | chr7.3:8653122-8653141 | MS.gene025631:CDS | 30.0% | |
| ! | TTAGAGCTTTTACTGAGATT+TGG | + | chr7.3:8653232-8653251 | MS.gene025631:CDS | 30.0% |
| !!! | AAGTTTGTGTTTTTGGTAAG+TGG | - | chr7.3:8652863-8652882 | None:intergenic | 30.0% |
| !!! | AGTTTGTGTTTTTGGTAAGT+GGG | - | chr7.3:8652862-8652881 | None:intergenic | 30.0% |
| AACAGTTCAAAAGCTCTCTA+AGG | - | chr7.3:8653173-8653192 | None:intergenic | 35.0% | |
| AATAACCAGTACTCTTCATC+TGG | + | chr7.3:8652886-8652905 | MS.gene025631:CDS | 35.0% | |
| ATAGTATGGTGAAAATGTGG+TGG | - | chr7.3:8652974-8652993 | None:intergenic | 35.0% | |
| GAATGCCTGAAATCAAGATA+AGG | - | chr7.3:8653105-8653124 | None:intergenic | 35.0% | |
| GGAATAGTATGGTGAAAATG+TGG | - | chr7.3:8652977-8652996 | None:intergenic | 35.0% | |
| TCATATTTGTCTTCAGTCCA+AGG | - | chr7.3:8652913-8652932 | None:intergenic | 35.0% | |
| TGATTCAGATGCTTATGCAA+AGG | + | chr7.3:8653008-8653027 | MS.gene025631:CDS | 35.0% | |
| TGTTTGTGGTTGTAGTGTTT+GGG | - | chr7.3:8652948-8652967 | None:intergenic | 35.0% | |
| ! | CTCCAATATCATTTGAAGCA+TGG | - | chr7.3:8653127-8653146 | None:intergenic | 35.0% |
| ! | GCTTTTACTGAGATTTGGAA+TGG | + | chr7.3:8653237-8653256 | MS.gene025631:CDS | 35.0% |
| !!! | GGTTCTTGGATTTTGGTTTT+GGG | - | chr7.3:8652840-8652859 | None:intergenic | 35.0% |
| !!! | GTTTGTGTTTTTGGTAAGTG+GGG | - | chr7.3:8652861-8652880 | None:intergenic | 35.0% |
| ATGCTTATGCAAAGGACCAA+AGG | + | chr7.3:8653016-8653035 | MS.gene025631:CDS | 40.0% | |
| CTGAATGCACCTTATCATCA+TGG | + | chr7.3:8653201-8653220 | MS.gene025631:CDS | 40.0% | |
| GGAATGGAGTTTCAACTATG+AGG | + | chr7.3:8653253-8653272 | MS.gene025631:CDS | 40.0% | |
| GTACGAGATGTATGCAAACT+GGG | - | chr7.3:8653288-8653307 | None:intergenic | 40.0% | |
| GTGTTTGTGGTTGTAGTGTT+TGG | - | chr7.3:8652949-8652968 | None:intergenic | 40.0% | |
| GTTTGTGGTTGTAGTGTTTG+GGG | - | chr7.3:8652947-8652966 | None:intergenic | 40.0% | |
| TTAATGTTGACGTGGCTTAC+GGG | - | chr7.3:8653322-8653341 | None:intergenic | 40.0% | |
| ! | TAAGTGGGGTTCTTGGATTT+TGG | - | chr7.3:8652847-8652866 | None:intergenic | 40.0% |
| !! | TTTTTGGTAAGTGGGGTTCT+TGG | - | chr7.3:8652854-8652873 | None:intergenic | 40.0% |
| !!! | GGGTTCTTGGATTTTGGTTT+TGG | - | chr7.3:8652841-8652860 | None:intergenic | 40.0% |
| AAATGTGGTGGCAGTGTTTG+TGG | - | chr7.3:8652962-8652981 | None:intergenic | 45.0% | |
| AGTACTCTTCATCTGGTCCT+TGG | + | chr7.3:8652893-8652912 | MS.gene025631:CDS | 45.0% | |
| CAAAGGAGAGTAGGTGGTTT+AGG | + | chr7.3:8653033-8653052 | MS.gene025631:CDS | 45.0% | |
| CTAAACCACCTACTCTCCTT+TGG | - | chr7.3:8653035-8653054 | None:intergenic | 45.0% | |
| GCTTCTTCTGAACACTGTGT+CGG | + | chr7.3:8652762-8652781 | MS.gene025631:CDS | 45.0% | |
| GGTACGAGATGTATGCAAAC+TGG | - | chr7.3:8653289-8653308 | None:intergenic | 45.0% | |
| GTATGCAAACTGGGTGAACT+AGG | - | chr7.3:8653279-8653298 | None:intergenic | 45.0% | |
| ! | AACACTGTGTCGGTGAAACT+AGG | + | chr7.3:8652772-8652791 | MS.gene025631:CDS | 45.0% |
| ! | AAGGACCAGATGAAGAGTAC+TGG | - | chr7.3:8652894-8652913 | None:intergenic | 45.0% |
| AAGGACCAAAGGAGAGTAGG+TGG | + | chr7.3:8653027-8653046 | MS.gene025631:CDS | 50.0% | |
| GCAAAGGACCAAAGGAGAGT+AGG | + | chr7.3:8653024-8653043 | MS.gene025631:CDS | 50.0% | |
| GTAGGTGGTTTAGGAAGAGC+TGG | + | chr7.3:8653042-8653061 | MS.gene025631:CDS | 50.0% | |
| TAGGTTGTGGAAGCTGCAGA+AGG | + | chr7.3:8652791-8652810 | MS.gene025631:CDS | 50.0% | |
| TTAGGAAGAGCTGGAGGAGA+AGG | + | chr7.3:8653051-8653070 | MS.gene025631:CDS | 50.0% | |
| ! | GTGTCGGTGAAACTAGGTTG+TGG | + | chr7.3:8652778-8652797 | MS.gene025631:CDS | 50.0% |
| AAGAGCTGGAGGAGAAGGTG+TGG | + | chr7.3:8653056-8653075 | MS.gene025631:CDS | 55.0% | |
| GGTGGTTTAGGAAGAGCTGG+AGG | + | chr7.3:8653045-8653064 | MS.gene025631:CDS | 55.0% | |
| GTTGACGTGGCTTACGGGTA+CGG | - | chr7.3:8653317-8653336 | None:intergenic | 55.0% | |
| TTGACGTGGCTTACGGGTAC+GGG | - | chr7.3:8653316-8653335 | None:intergenic | 55.0% | |
| GTGGCTTACGGGTACGGGTA+CGG | - | chr7.3:8653311-8653330 | None:intergenic | 60.0% | |
| TGGCTTACGGGTACGGGTAC+GGG | - | chr7.3:8653310-8653329 | None:intergenic | 60.0% | |
| ! | AGCTGGAGGAGAAGGTGTGG+CGG | + | chr7.3:8653059-8653078 | MS.gene025631:CDS | 60.0% |
| ! | TGGAGGAGAAGGTGTGGCGG+TGG | + | chr7.3:8653062-8653081 | MS.gene025631:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 8652742 | 8653341 | 8652742 | ID=MS.gene025631 |
| chr7.3 | mRNA | 8652742 | 8653341 | 8652742 | ID=MS.gene025631.t1;Parent=MS.gene025631 |
| chr7.3 | exon | 8652742 | 8653341 | 8652742 | ID=MS.gene025631.t1.exon1;Parent=MS.gene025631.t1 |
| chr7.3 | CDS | 8652742 | 8653341 | 8652742 | ID=cds.MS.gene025631.t1;Parent=MS.gene025631.t1 |
| Gene Sequence |
| Protein sequence |
