AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025665

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025665.t1 MTR_7g106320 97.001 667 13 1 1 667 1 660 0.0 1336
MS.gene025665.t1 MTR_1g057790 67.054 689 165 20 1 667 1 649 0.0 814
MS.gene025665.t1 MTR_4g051532 45.455 759 274 24 1 667 1 711 1.19e-164 489
MS.gene025665.t1 MTR_1g023050 56.146 301 97 8 153 433 146 431 5.41e-100 319
MS.gene025665.t1 MTR_3g112300 54.676 278 94 6 172 435 163 422 2.44e-92 296
MS.gene025665.t1 MTR_3g112300 54.676 278 94 6 172 435 163 422 1.35e-91 296
MS.gene025665.t1 MTR_8g078480 53.929 280 122 2 172 447 389 665 1.73e-86 289
MS.gene025665.t1 MTR_5g018860 46.061 330 156 4 172 490 339 657 4.32e-86 286
MS.gene025665.t1 MTR_8g098815 52.416 269 115 4 160 421 170 432 1.26e-80 268
MS.gene025665.t1 MTR_3g112300 52.419 248 86 6 172 405 163 392 6.33e-75 246
MS.gene025665.t1 MTR_3g112290 46.622 296 129 7 169 451 163 442 2.10e-71 238
MS.gene025665.t1 MTR_4g019450 42.675 314 137 6 175 481 200 477 4.28e-65 226
MS.gene025665.t1 MTR_7g065050 43.796 274 129 6 175 445 141 392 3.46e-62 216
MS.gene025665.t1 MTR_3g112300 49.780 227 82 6 172 384 163 371 7.84e-62 211
MS.gene025665.t1 MTR_2g095050 38.253 332 165 7 118 430 201 511 2.22e-61 216
MS.gene025665.t1 MTR_1g016490 38.519 270 132 7 172 427 244 493 1.91e-48 178
MS.gene025665.t1 MTR_7g114930 70.270 37 11 0 547 583 9 45 2.89e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025665.t1 AT2G35940 48.797 748 234 33 1 667 1 680 7.91e-170 501
MS.gene025665.t1 AT2G35940 48.797 748 234 33 1 667 1 680 7.91e-170 501
MS.gene025665.t1 AT2G35940 48.797 748 234 33 1 667 1 680 7.91e-170 501
MS.gene025665.t1 AT2G16400 52.769 307 113 5 163 455 98 386 1.02e-96 306
MS.gene025665.t1 AT4G34610 52.308 325 129 7 132 446 94 402 1.46e-92 297
MS.gene025665.t1 AT4G34610 52.308 325 129 7 132 446 94 402 1.46e-92 297
MS.gene025665.t1 AT4G34610 52.308 325 129 7 132 446 94 402 1.46e-92 297
MS.gene025665.t1 AT4G34610 52.308 325 129 7 132 446 94 402 1.46e-92 297
MS.gene025665.t1 AT1G75410 53.608 291 114 7 172 458 168 441 3.13e-92 296
MS.gene025665.t1 AT1G75410 53.608 291 114 7 172 458 168 441 3.13e-92 296
MS.gene025665.t1 AT1G19700 55.017 289 112 8 162 441 156 435 2.26e-89 289
MS.gene025665.t1 AT1G19700 55.017 289 112 8 162 441 156 435 2.26e-89 289
MS.gene025665.t1 AT1G19700 55.017 289 112 8 162 441 156 435 2.26e-89 289
MS.gene025665.t1 AT1G19700 55.017 289 112 8 162 441 156 435 2.26e-89 289
MS.gene025665.t1 AT1G19700 55.017 289 112 8 162 441 156 435 2.26e-89 289
MS.gene025665.t1 AT2G27220 51.211 289 98 7 157 442 64 312 2.70e-82 267
MS.gene025665.t1 AT2G27220 51.211 289 98 7 157 442 64 312 2.70e-82 267
MS.gene025665.t1 AT4G36870 49.502 301 124 5 170 449 310 603 2.88e-79 267
MS.gene025665.t1 AT4G36870 49.502 301 124 5 170 449 310 603 2.88e-79 267
MS.gene025665.t1 AT2G27220 48.208 307 98 8 157 442 64 330 1.97e-78 257
MS.gene025665.t1 AT2G23760 50.758 264 117 3 169 425 234 491 3.41e-76 256
MS.gene025665.t1 AT2G23760 50.758 264 117 3 169 425 234 491 3.41e-76 256
MS.gene025665.t1 AT2G23760 50.758 264 117 3 169 425 234 491 3.41e-76 256
MS.gene025665.t1 AT5G41410 44.795 317 161 5 170 472 192 508 4.70e-76 255
MS.gene025665.t1 AT1G75410 53.361 238 95 6 172 405 168 393 2.56e-73 242
MS.gene025665.t1 AT2G23760 42.405 316 117 4 169 425 234 543 3.50e-66 230
MS.gene025665.t1 AT4G36870 49.180 244 110 4 170 406 310 546 4.55e-63 219
MS.gene025665.t1 AT4G36870 48.347 242 111 4 170 404 310 544 6.17e-62 216
MS.gene025665.t1 AT1G75430 40.830 289 143 7 172 448 17 289 1.27e-60 205
MS.gene025665.t1 AT5G02030 54.595 185 75 3 253 428 234 418 3.33e-57 204
MS.gene025665.t1 AT4G32980 39.700 267 142 5 172 437 202 450 7.73e-52 186
MS.gene025665.t1 AT4G32980 39.700 267 142 5 172 437 202 450 7.73e-52 186
MS.gene025665.t1 AT2G27990 38.081 344 158 12 106 430 189 496 2.77e-51 187
MS.gene025665.t1 AT1G75430 34.177 237 107 5 172 384 17 228 3.57e-32 126
MS.gene025665.t1 AT2G27990 31.879 298 148 12 106 384 189 450 5.64e-25 108

Find 173 sgRNAs with CRISPR-Local

Find 286 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGAGTTCAAAGTATTTA+AGG 0.159295 7.1:-6454963 MS.gene025665:CDS
TTAACATCAATGAAACTATT+AGG 0.199947 7.1:+6451702 None:intergenic
ATCTTTCAGGCAATCCTCTT+TGG 0.211678 7.1:+6452582 None:intergenic
TCTTTCAGGCAATCCTCTTT+GGG 0.217697 7.1:+6452583 None:intergenic
TATTGGAGTTTCATGGCTTT+TGG 0.219698 7.1:+6451869 None:intergenic
ACAAACTTCATTGGTGGATT+TGG 0.220206 7.1:-6451591 MS.gene025665:CDS
AACTGTGCAGCAAACCTCTT+TGG 0.253744 7.1:+6451336 None:intergenic
TAAAGCTACAAATAAGAGTT+TGG 0.254411 7.1:-6452726 MS.gene025665:CDS
TTCTTCCTAGTTGAATGTTT+TGG 0.269559 7.1:+6451439 None:intergenic
AATTCAGGATTCAGCTTCAT+TGG 0.282072 7.1:-6451795 MS.gene025665:CDS
GAGGAACTATCAATGAAATT+TGG 0.286124 7.1:-6451660 MS.gene025665:CDS
GGTCTAGAAACAGAAATTAT+TGG 0.288557 7.1:+6451852 None:intergenic
ATGAAGGAAAATGAACTAAA+TGG 0.289721 7.1:-6452011 MS.gene025665:CDS
GTATCACGCGCCGCCGTGTT+TGG 0.299903 7.1:+6455120 None:intergenic
CGCCGCCGCCACCACTTGAT+TGG 0.300629 7.1:+6455442 None:intergenic
AATTGCAACAGGTTTCAAAT+TGG 0.308221 7.1:-6452094 MS.gene025665:intron
GGAAATGTTAGAAACAATTC+AGG 0.311379 7.1:-6451810 MS.gene025665:CDS
GGTTCGCCGTCTCCGGCTTC+TGG 0.313206 7.1:-6455027 MS.gene025665:CDS
GGAGGAGATGGATCAATAAT+TGG 0.316947 7.1:-6454823 MS.gene025665:CDS
CTGTTTCTGTTCTTCGTGCT+TGG 0.318849 7.1:-6452559 MS.gene025665:CDS
CAATACCCAATTGGTGATAT+TGG 0.320766 7.1:-6451567 MS.gene025665:CDS
CAAAACAAACAGGGCTTACT+AGG 0.321411 7.1:-6452207 MS.gene025665:CDS
TAACATCAATGAAACTATTA+GGG 0.331113 7.1:+6451703 None:intergenic
CAGAAATTATTGGAGTTTCA+TGG 0.342483 7.1:+6451862 None:intergenic
ACAACGTGCTCTACAACAAT+TGG 0.343052 7.1:-6452633 MS.gene025665:CDS
ATCCACCAATGAAGTTTGTT+TGG 0.345852 7.1:+6451595 None:intergenic
GAATAATAACAATGGGAATC+CGG 0.361123 7.1:-6454995 MS.gene025665:CDS
TAGGGTTATGCATAAGTTCA+TGG 0.365082 7.1:+6451721 None:intergenic
ATTATTTAGGACACAAAATC+TGG 0.366418 7.1:+6451309 None:intergenic
ATAAGTGAACCAAATGAGTT+TGG 0.373207 7.1:-6451408 MS.gene025665:CDS
CACATGCTTGCAAAACAAAC+AGG 0.376982 7.1:-6452217 MS.gene025665:CDS
CGTCATGGTGCGCGTGGATT+TGG 0.380286 7.1:+6455205 None:intergenic
GGTGAAGAGGATTGCTTTGG+AGG 0.380432 7.1:-6452704 MS.gene025665:CDS
TGTCCTAGCTGAACCGATTC+CGG 0.381221 7.1:+6452820 None:intergenic
TTGGGTGAAGAGGATTGCTT+TGG 0.382173 7.1:-6452707 MS.gene025665:CDS
TTTATAGAACCAAACTCATT+TGG 0.385263 7.1:+6451399 None:intergenic
TGTGAATAGTGGTGTTGAAT+TGG 0.390097 7.1:-6454908 MS.gene025665:CDS
AACCTACCAATATCACCAAT+TGG 0.390271 7.1:+6451561 None:intergenic
ATTGGATCATCAGAACTAGA+AGG 0.393321 7.1:-6451777 MS.gene025665:CDS
AAAGCTACAAATAAGAGTTT+GGG 0.393739 7.1:-6452725 MS.gene025665:CDS
TGTGTGGAATTGTAACCTTT+AGG 0.394460 7.1:+6451900 None:intergenic
GAGGTAAAATTGAAGGTTCA+AGG 0.397374 7.1:-6452685 MS.gene025665:CDS
ACTGTGCAGCAAACCTCTTT+GGG 0.397435 7.1:+6451337 None:intergenic
TATCACGCGCCGCCGTGTTT+GGG 0.400157 7.1:+6455121 None:intergenic
TGGTGTTGTTGGTGGTTAAG+AGG 0.400409 7.1:+6455225 None:intergenic
TGCATAAGTTCATGGTTCCT+TGG 0.400560 7.1:+6451729 None:intergenic
GCAAGATTCGTTTCTATGTC+TGG 0.404625 7.1:-6455051 MS.gene025665:CDS
GACATAGAAACGAATCTTGC+CGG 0.407425 7.1:+6455054 None:intergenic
TAAATGCTCGAGTCAGGCTT+TGG 0.408513 7.1:-6452067 MS.gene025665:CDS
GCGCGTGGATTTGGTGTTGT+TGG 0.411629 7.1:+6455214 None:intergenic
TCTGAGTTCAAAGTATTTAA+GGG 0.411868 7.1:-6454962 MS.gene025665:CDS
GTTGAGGAAAACATAGTTAC+CGG 0.412150 7.1:+6455320 None:intergenic
GGAATGAGCAAGGTGAATAT+TGG 0.415474 7.1:-6454868 MS.gene025665:CDS
GGAAACCAAACAAACTTCAT+TGG 0.423223 7.1:-6451600 MS.gene025665:CDS
GGTTTATAAATGCTCGAGTC+AGG 0.425092 7.1:-6452073 MS.gene025665:CDS
GGGGTCTCCAAGCGTTATGC+TGG 0.431536 7.1:+6452603 None:intergenic
AGAACAGAAACAGATCTTTC+AGG 0.435978 7.1:+6452569 None:intergenic
CTTGGTCTTGATTCAATACA+AGG 0.436754 7.1:-6451486 MS.gene025665:CDS
AATAATAACAATGGGAATCC+GGG 0.437333 7.1:-6454994 MS.gene025665:CDS
TCAATAATTGGTGGTGGGGA+GGG 0.443667 7.1:-6454811 MS.gene025665:CDS
GTGAATATTGGAGAATCATC+AGG 0.448018 7.1:-6454856 MS.gene025665:CDS
TTCGAACAAGCCGCCGGAAT+CGG 0.449398 7.1:-6452833 MS.gene025665:CDS
ATTGGTGGTGGGGAGGGAAG+TGG 0.452026 7.1:-6454805 MS.gene025665:CDS
ATCAATAATTGGTGGTGGGG+AGG 0.457926 7.1:-6454812 MS.gene025665:CDS
AAACAATCTCAAAGCAGTTT+CGG 0.458580 7.1:-6452778 MS.gene025665:CDS
TGCTTTGGAGGTAAAATTGA+AGG 0.465481 7.1:-6452692 MS.gene025665:CDS
AAACCACAACCTCCATTTGA+TGG 0.467737 7.1:+6452861 None:intergenic
GTCTGGTGGTTCGCCGTCTC+CGG 0.468228 7.1:-6455034 MS.gene025665:CDS
GTGAATAGTGGTGTTGAATT+GGG 0.472967 7.1:-6454907 MS.gene025665:CDS
TGTTTGAAAGATGCAATAAC+AGG 0.474143 7.1:-6452755 MS.gene025665:CDS
CCACAATCATCACCACTTGT+TGG 0.474765 7.1:-6451831 MS.gene025665:CDS
ATGTTGATACTTTCATATTG+AGG 0.481269 7.1:+6451366 None:intergenic
CACACATGCAGTACAATCAA+TGG 0.483257 7.1:-6455155 MS.gene025665:CDS
ATGTGCTTGTCTGAATCCTT+TGG 0.484894 7.1:+6452235 None:intergenic
AATAATGTTTCACTCACTCT+TGG 0.485275 7.1:-6451504 MS.gene025665:CDS
CAACCTTCTCATGCCGCTGC+CGG 0.488739 7.1:-6455339 MS.gene025665:CDS
TCAGAATATTGTATATAGCC+TGG 0.489333 7.1:+6455387 None:intergenic
TTACAACGAATAATAACAAT+GGG 0.489888 7.1:-6455002 MS.gene025665:CDS
GACGTTGTACCTTCTTTGCA+TGG 0.491466 7.1:-6455186 MS.gene025665:CDS
AGTGGTGGCGGCGGCGCCGA+CGG 0.491733 7.1:-6455435 MS.gene025665:CDS
CCATGATAGTACGTTGCCAT+TGG 0.498403 7.1:+6455480 None:intergenic
CCACCATCAAATGGAGGTTG+TGG 0.501135 7.1:-6452864 MS.gene025665:CDS
ACCTACCAATATCACCAATT+GGG 0.505940 7.1:+6451562 None:intergenic
CAGACAGTACCACCATCAAA+TGG 0.506484 7.1:-6452873 MS.gene025665:CDS
AACAGGGCTTACTAGGAGTC+AGG 0.508298 7.1:-6452200 MS.gene025665:intron
AACCAAACAAACTTCATTGG+TGG 0.508445 7.1:-6451597 MS.gene025665:CDS
ACCTTTAGGTTCTGTTTCAG+AGG 0.511607 7.1:+6451914 None:intergenic
ACGTACATTTCTTCCACCAT+TGG 0.511797 7.1:+6452041 None:intergenic
TTTCTAACATTTCCAACAAG+TGG 0.513849 7.1:+6451819 None:intergenic
ATCCAATCAAGTGGTGGCGG+CGG 0.518066 7.1:-6455444 MS.gene025665:CDS
GAACTCAGAATCTGAATCCC+CGG 0.518621 7.1:+6454976 None:intergenic
CAACGTGCTCTACAACAATT+GGG 0.519313 7.1:-6452632 MS.gene025665:CDS
CTTTCAGGCAATCCTCTTTG+GGG 0.520504 7.1:+6452584 None:intergenic
AAATAAGAGTTTGGGTGAAG+AGG 0.522513 7.1:-6452717 MS.gene025665:CDS
GATGGATCAATAATTGGTGG+TGG 0.523887 7.1:-6454817 MS.gene025665:CDS
AATGTACGTGGAAGAAATGA+AGG 0.525935 7.1:-6452027 MS.gene025665:CDS
CGTGGATTTGGTGTTGTTGG+TGG 0.528203 7.1:+6455217 None:intergenic
GAATCATCAGGAGTTGGTAG+TGG 0.530198 7.1:-6454844 MS.gene025665:CDS
AGGTGGCGCGTGAGGTGAGA+AGG 0.530811 7.1:+6455275 None:intergenic
GGAAGGAAGCCATGCAAAGA+AGG 0.533172 7.1:+6455177 None:intergenic
TTCGTTGTAACACCAGAAGC+CGG 0.536997 7.1:+6455015 None:intergenic
CCAATGGCAACGTACTATCA+TGG 0.542137 7.1:-6455480 MS.gene025665:CDS
ACATTATTACTTGAAAACCT+TGG 0.542336 7.1:+6451519 None:intergenic
GGATTTGGACAATACCCAAT+TGG 0.542874 7.1:-6451576 MS.gene025665:CDS
GGAGTTGGTAGTGGAGGAGA+TGG 0.547177 7.1:-6454835 MS.gene025665:CDS
AAAAGTGTACTTACGGGTGG+AGG 0.547866 7.1:+6452522 None:intergenic
ACACTTGTTCTCATGAATCC+AGG 0.550346 7.1:-6455405 MS.gene025665:CDS
TCCTCGTTCGAACAAGCCGC+CGG 0.551758 7.1:-6452839 MS.gene025665:CDS
ACCCAATTGGTGATATTGGT+AGG 0.552228 7.1:-6451563 MS.gene025665:CDS
GAATGATCCAGCATAACGCT+TGG 0.554984 7.1:-6452610 MS.gene025665:CDS
TCCAACACTCAACAATTCGT+TGG 0.555631 7.1:-6455252 MS.gene025665:CDS
ACCTCTGAAACAGAACCTAA+AGG 0.555839 7.1:-6451915 MS.gene025665:CDS
TCTTAGTCTCTCTTCACAGC+CGG 0.560177 7.1:-6455073 MS.gene025665:CDS
CATTGATTGTACTGCATGTG+TGG 0.560839 7.1:+6455156 None:intergenic
TTGAGATTGTTTGTAGTGCA+AGG 0.562020 7.1:+6452789 None:intergenic
CCGCCGGAATCGGTTCAGCT+AGG 0.562168 7.1:-6452823 MS.gene025665:CDS
ATTGGAGAATCATCAGGAGT+TGG 0.562542 7.1:-6454850 MS.gene025665:CDS
TTGGGAAAGAAGAGTGGTGG+TGG 0.562954 7.1:-6454889 MS.gene025665:CDS
GAAAACATAGTTACCGGCAG+CGG 0.567137 7.1:+6455326 None:intergenic
TCATCAGGAGTTGGTAGTGG+AGG 0.567311 7.1:-6454841 MS.gene025665:CDS
GTTACAACGAATAATAACAA+TGG 0.571166 7.1:-6455003 MS.gene025665:CDS
GCCAACGAATTGTTGAGTGT+TGG 0.571349 7.1:+6455251 None:intergenic
ATGGATCAATAATTGGTGGT+GGG 0.575349 7.1:-6454816 MS.gene025665:CDS
ATGAAATACCTCATCAAGCA+TGG 0.576005 7.1:+6454714 None:intergenic
TGAGATTGTTTGTAGTGCAA+GGG 0.576129 7.1:+6452790 None:intergenic
GCACAATGAGGCAAATAATG+AGG 0.576266 7.1:-6451679 MS.gene025665:CDS
CCAAACCAAAACATTCAACT+AGG 0.579234 7.1:-6451444 MS.gene025665:CDS
GAAATCCAATCAAGTGGTGG+CGG 0.581239 7.1:-6455447 MS.gene025665:CDS
TTTCAGCAGCTGTAGAGTTG+AGG 0.581659 7.1:+6455304 None:intergenic
CATTGATGTTAAGCACAATG+AGG 0.585091 7.1:-6451691 MS.gene025665:CDS
GTTGGTTGAAGGTGGAGCAA+AGG 0.585799 7.1:-6452898 MS.gene025665:intron
GATGAGAGACAAAATAGAGA+TGG 0.586332 7.1:-6451636 MS.gene025665:CDS
TTACCGGCAGCGGCATGAGA+AGG 0.586375 7.1:+6455336 None:intergenic
TCAACATGCAAAACCCAAAG+AGG 0.590340 7.1:-6451350 MS.gene025665:CDS
AACAAGTGTTTGAAGTCCGT+CGG 0.592444 7.1:+6455419 None:intergenic
TTGGATCATCAGAACTAGAA+GGG 0.594241 7.1:-6451776 MS.gene025665:CDS
AGTCAGGCTTTGGAAGCCAA+TGG 0.594390 7.1:-6452057 MS.gene025665:CDS
GAGTGGTGGTGGAATGAGCA+AGG 0.594607 7.1:-6454878 MS.gene025665:CDS
GCTAATTACCATGCTTGATG+AGG 0.595737 7.1:-6454722 MS.gene025665:intron
GTACAACGTCATGGTGCGCG+TGG 0.595803 7.1:+6455199 None:intergenic
GACAGTCCTTGTTGAGCGCG+TGG 0.597975 7.1:+6455096 None:intergenic
GATTGTACTGCATGTGTGGA+AGG 0.599960 7.1:+6455160 None:intergenic
CCAACAAGTGGTGATGATTG+TGG 0.604006 7.1:+6451831 None:intergenic
AAATGAACTAAATGGATCTG+AGG 0.605958 7.1:-6452003 MS.gene025665:CDS
TGGATCAATAATTGGTGGTG+GGG 0.613381 7.1:-6454815 MS.gene025665:CDS
GATACTTTCATATTGAGGAG+AGG 0.614077 7.1:+6451371 None:intergenic
AATTCTGAAATCCAATCAAG+TGG 0.614202 7.1:-6455453 MS.gene025665:CDS
AGATTCGTTTCTATGTCTGG+TGG 0.614647 7.1:-6455048 MS.gene025665:CDS
GAACACAATTGACCCAAACA+CGG 0.622706 7.1:-6455133 MS.gene025665:CDS
AATTGTTGAGTGTTGGAAGG+TGG 0.623899 7.1:+6455258 None:intergenic
GCAAAGAAGGTACAACGTCA+TGG 0.625334 7.1:+6455190 None:intergenic
GATACACCACGCGCTCAACA+AGG 0.625868 7.1:-6455102 MS.gene025665:CDS
CAGGCTTTGGAAGCCAATGG+TGG 0.629052 7.1:-6452054 MS.gene025665:CDS
GAGGTTGTGAATGTGAATAG+TGG 0.635951 7.1:-6454919 MS.gene025665:CDS
ACGAATTGTTGAGTGTTGGA+AGG 0.639184 7.1:+6455255 None:intergenic
GTAACACCAGAAGCCGGAGA+CGG 0.643060 7.1:+6455021 None:intergenic
GTGTTGGAAGGTGGCGCGTG+AGG 0.647584 7.1:+6455267 None:intergenic
ATAATAACAATGGGAATCCG+GGG 0.647699 7.1:-6454993 MS.gene025665:CDS
CCACAACCTCCATTTGATGG+TGG 0.650106 7.1:+6452864 None:intergenic
TCCGGCGGCTTGTTCGAACG+AGG 0.652183 7.1:+6452838 None:intergenic
ACATGCTTGCAAAACAAACA+GGG 0.653038 7.1:-6452216 MS.gene025665:CDS
ACTCAGAACAATTCACACCA+AGG 0.656664 7.1:-6451536 MS.gene025665:CDS
TCTGAAATCCAATCAAGTGG+TGG 0.660822 7.1:-6455450 MS.gene025665:CDS
GTTGAATTGGGAAAGAAGAG+TGG 0.679581 7.1:-6454895 MS.gene025665:CDS
GGAGATGGATCAATAATTGG+TGG 0.682848 7.1:-6454820 MS.gene025665:CDS
AACGCTTGGAGACCCCAAAG+AGG 0.685460 7.1:-6452596 MS.gene025665:CDS
GAACTAGAAGGGATACCACA+AGG 0.698540 7.1:-6451765 MS.gene025665:CDS
GAATTGGGAAAGAAGAGTGG+TGG 0.708823 7.1:-6454892 MS.gene025665:CDS
ACAGTACCACCATCAAATGG+AGG 0.710282 7.1:-6452870 MS.gene025665:CDS
CACAATTGACCCAAACACGG+CGG 0.712861 7.1:-6455130 MS.gene025665:CDS
CCTAGCTGAACCGATTCCGG+CGG 0.726597 7.1:+6452823 None:intergenic
ATGGCAACGTACTATCATGG+TGG 0.734896 7.1:-6455477 MS.gene025665:CDS
AATGGTGGAAGAAATGTACG+TGG 0.752334 7.1:-6452039 MS.gene025665:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTAATTAAATTTTTAT+AGG - chr7.1:6452349-6452368 MS.gene025665:intron 0.0%
!! AAAATGTAATTATAAAAATG+AGG + chr7.1:6453807-6453826 None:intergenic 10.0%
!! TAAAATATTGAAATGATTTA+TGG + chr7.1:6453678-6453697 None:intergenic 10.0%
!!! AAAAGTTTAGTATAATTTTT+TGG - chr7.1:6452261-6452280 MS.gene025665:intron 10.0%
!!! ACTTTTATAATGAATTAAAA+TGG + chr7.1:6452287-6452306 None:intergenic 10.0%
!!! TAATTACATTTTGAAATTAA+CGG - chr7.1:6453815-6453834 MS.gene025665:intron 10.0%
!!! TTAACAATTTTTTTTTTAAC+TGG - chr7.1:6452606-6452625 MS.gene025665:CDS 10.0%
!! TGTGATAAAATATTAATGTA+AGG + chr7.1:6452789-6452808 None:intergenic 15.0%
!! TGTTTATGATATATACTATT+TGG - chr7.1:6453166-6453185 MS.gene025665:intron 15.0%
!!! AATACAATCTTTTAAAGTAA+AGG - chr7.1:6453460-6453479 MS.gene025665:intron 15.0%
!!! GTAATCAATCAATATAATTT+TGG - chr7.1:6454613-6454632 MS.gene025665:intron 15.0%
!! ACTAAAATGAATTTACTTGT+TGG - chr7.1:6453875-6453894 MS.gene025665:intron 20.0%
!! ATAGCAATAAATGACATAAA+TGG - chr7.1:6453436-6453455 MS.gene025665:intron 20.0%
!! ATATATCATAAACACCAAAT+AGG + chr7.1:6453160-6453179 None:intergenic 20.0%
!! ATTTGAATATGCCAAAAATA+AGG + chr7.1:6454651-6454670 None:intergenic 20.0%
!! CATCAAGGTTAAATTATAAA+TGG + chr7.1:6452883-6452902 None:intergenic 20.0%
!! TAACATCAATGAAACTATTA+GGG + chr7.1:6455091-6455110 None:intergenic 20.0%
!! TTAACATCAATGAAACTATT+AGG + chr7.1:6455092-6455111 None:intergenic 20.0%
!! TTACAACGAATAATAACAAT+GGG - chr7.1:6451789-6451808 MS.gene025665:CDS 20.0%
!! TTTCAATAAAACTAATTGCA+TGG - chr7.1:6453702-6453721 MS.gene025665:intron 20.0%
!!! AAATTAGAAAAGTGTACTTA+CGG + chr7.1:6454279-6454298 None:intergenic 20.0%
!!! AATAGTGAATTTTCATTAGA+TGG + chr7.1:6453774-6453793 None:intergenic 20.0%
!!! ACAATTTCTTTCCTTATTTT+TGG - chr7.1:6454637-6454656 MS.gene025665:intron 20.0%
!!! GAGTCATTTAGTAAATTTTT+TGG - chr7.1:6453212-6453231 MS.gene025665:intron 20.0%
!!! TAATATTTTATCACAGTCAA+TGG - chr7.1:6452794-6452813 MS.gene025665:CDS 20.0%
!!! TATTTTCTCTAACACTTTAT+TGG + chr7.1:6454350-6454369 None:intergenic 20.0%
! AAAAACTACTCTTGTACTTA+AGG - chr7.1:6452674-6452693 MS.gene025665:CDS 25.0%
! AAAGCTACAAATAAGAGTTT+GGG - chr7.1:6454066-6454085 MS.gene025665:intron 25.0%
! ACATTATTACTTGAAAACCT+TGG + chr7.1:6455275-6455294 None:intergenic 25.0%
! ACTTATAACTCAACATGATT+TGG - chr7.1:6452733-6452752 MS.gene025665:CDS 25.0%
! ACTTATTGATACCAAATCTA+AGG - chr7.1:6453098-6453117 MS.gene025665:intron 25.0%
! ATGAAGGAAAATGAACTAAA+TGG - chr7.1:6454780-6454799 MS.gene025665:CDS 25.0%
! ATGTTGATACTTTCATATTG+AGG + chr7.1:6455428-6455447 None:intergenic 25.0%
! GTTACAACGAATAATAACAA+TGG - chr7.1:6451788-6451807 MS.gene025665:CDS 25.0%
! TAAAGCTACAAATAAGAGTT+TGG - chr7.1:6454065-6454084 MS.gene025665:intron 25.0%
! TAATCAATATCACATTCTTC+CGG + chr7.1:6453245-6453264 None:intergenic 25.0%
! TTTATAGAACCAAACTCATT+TGG + chr7.1:6455395-6455414 None:intergenic 25.0%
!! AATTAGAAAAGTGTACTTAC+GGG + chr7.1:6454278-6454297 None:intergenic 25.0%
!! TCTGAGTTCAAAGTATTTAA+GGG - chr7.1:6451829-6451848 MS.gene025665:CDS 25.0%
!! TTCTGAGTTCAAAGTATTTA+AGG - chr7.1:6451828-6451847 MS.gene025665:CDS 25.0%
!!! AATTTTGTTTGAATTGCAAC+AGG - chr7.1:6454686-6454705 MS.gene025665:intron 25.0%
!!! ATTAGGAATAGTTATTTCTC+TGG - chr7.1:6453038-6453057 MS.gene025665:intron 25.0%
!!! TAAATTTTTTGGTGTTAGAC+CGG - chr7.1:6453223-6453242 MS.gene025665:intron 25.0%
!!! TCAATTTTTAGCTTGAAACT+TGG - chr7.1:6453344-6453363 MS.gene025665:intron 25.0%
!!! TTAGTCTTAGGTTTCTAATT+AGG - chr7.1:6453021-6453040 MS.gene025665:intron 25.0%
!!! TTTAACTTTTTATCTCTACG+TGG - chr7.1:6452418-6452437 MS.gene025665:intron 25.0%
AAACAATCTCAAAGCAGTTT+CGG - chr7.1:6454013-6454032 MS.gene025665:intron 30.0%
AAATGAACTAAATGGATCTG+AGG - chr7.1:6454788-6454807 MS.gene025665:CDS 30.0%
AAGACTAATATACCCAACAA+TGG + chr7.1:6453009-6453028 None:intergenic 30.0%
AATAATAACAATGGGAATCC+GGG - chr7.1:6451797-6451816 MS.gene025665:CDS 30.0%
AATAATGTTTCACTCACTCT+TGG - chr7.1:6455287-6455306 MS.gene025665:CDS 30.0%
AATGATAGAAAAACTGTGAG+TGG + chr7.1:6452547-6452566 None:intergenic 30.0%
AATTGCAACAGGTTTCAAAT+TGG - chr7.1:6454697-6454716 MS.gene025665:intron 30.0%
ATAAGTGAACCAAATGAGTT+TGG - chr7.1:6455383-6455402 MS.gene025665:CDS 30.0%
ATCAAGAAGGTAGTCTAAAT+GGG + chr7.1:6452908-6452927 None:intergenic 30.0%
ATTTAGACTACCTTCTTGAT+AGG - chr7.1:6452908-6452927 MS.gene025665:intron 30.0%
ATTTATACATCTTGAGCACT+TGG + chr7.1:6454451-6454470 None:intergenic 30.0%
CAGAAATTATTGGAGTTTCA+TGG + chr7.1:6454932-6454951 None:intergenic 30.0%
CATATACTGGTATTATTGCT+AGG - chr7.1:6452851-6452870 MS.gene025665:CDS 30.0%
CCCTATTTGTATTAATCACA+CGG - chr7.1:6453388-6453407 MS.gene025665:intron 30.0%
CCGTGTGATTAATACAAATA+GGG + chr7.1:6453391-6453410 None:intergenic 30.0%
CCTATTTGTATTAATCACAC+GGG - chr7.1:6453389-6453408 MS.gene025665:intron 30.0%
CTATCATTCGAGTATTTAAG+CGG - chr7.1:6452559-6452578 MS.gene025665:CDS 30.0%
CTTGAAACTTGGATATACAT+AGG - chr7.1:6453355-6453374 MS.gene025665:intron 30.0%
GAATAATAACAATGGGAATC+CGG - chr7.1:6451796-6451815 MS.gene025665:CDS 30.0%
GAGGAACTATCAATGAAATT+TGG - chr7.1:6455131-6455150 MS.gene025665:CDS 30.0%
GCTATTCCTTGCAATTTATA+AGG + chr7.1:6452832-6452851 None:intergenic 30.0%
GGAAATGTTAGAAACAATTC+AGG - chr7.1:6454981-6455000 MS.gene025665:CDS 30.0%
GGTCTAGAAACAGAAATTAT+TGG + chr7.1:6454942-6454961 None:intergenic 30.0%
TACTCTTGTACTTAAGGAAA+GGG - chr7.1:6452680-6452699 MS.gene025665:CDS 30.0%
TATCAAGAAGGTAGTCTAAA+TGG + chr7.1:6452909-6452928 None:intergenic 30.0%
TCAGAATATTGTATATAGCC+TGG + chr7.1:6451407-6451426 None:intergenic 30.0%
TGTTTGAAAGATGCAATAAC+AGG - chr7.1:6454036-6454055 MS.gene025665:intron 30.0%
TTTCTAACATTTCCAACAAG+TGG + chr7.1:6454975-6454994 None:intergenic 30.0%
! ACTCTAAAGAAAGGTTTGTT+AGG - chr7.1:6452969-6452988 MS.gene025665:intron 30.0%
! AGAGATGGTTACTCTTTTAT+GGG - chr7.1:6455170-6455189 MS.gene025665:CDS 30.0%
! CTTAAGTTACACCTTTTCAA+AGG + chr7.1:6453545-6453564 None:intergenic 30.0%
! TAGAGATGGTTACTCTTTTA+TGG - chr7.1:6455169-6455188 MS.gene025665:CDS 30.0%
! TCTTCACTTTCATGTGATTT+TGG - chr7.1:6452106-6452125 MS.gene025665:intron 30.0%
! TGAATTTACTTGTTGGTTGA+AGG - chr7.1:6453882-6453901 MS.gene025665:intron 30.0%
! TTCTTCCTAGTTGAATGTTT+TGG + chr7.1:6455355-6455374 None:intergenic 30.0%
! TTTAGAGTATCTCTAGCTAA+TGG + chr7.1:6452957-6452976 None:intergenic 30.0%
!! AATTCTGAAATCCAATCAAG+TGG - chr7.1:6451338-6451357 MS.gene025665:CDS 30.0%
!! ATTTGTTTTCAGCTATCCAA+AGG - chr7.1:6454540-6454559 MS.gene025665:intron 30.0%
!! CTCTAAAGAAAGGTTTGTTA+GGG - chr7.1:6452970-6452989 MS.gene025665:intron 30.0%
!! GTTGAGTTTTTCCTTTAGAA+AGG + chr7.1:6452764-6452783 None:intergenic 30.0%
!! TAACTCAACATGATTTGGTT+TGG - chr7.1:6452738-6452757 MS.gene025665:CDS 30.0%
!! TTGTTGGGTATATTAGTCTT+AGG - chr7.1:6453009-6453028 MS.gene025665:intron 30.0%
!!! CAAGAGTAGTTTTTTTTCCA+AGG + chr7.1:6452668-6452687 None:intergenic 30.0%
!!! GAGAATTGTTTTTGCTAAAG+TGG - chr7.1:6452440-6452459 MS.gene025665:intron 30.0%
!!! TTTTTTCCAAGGTGTTGAAT+TGG + chr7.1:6452657-6452676 None:intergenic 30.0%
AAATAAGAGTTTGGGTGAAG+AGG - chr7.1:6454074-6454093 MS.gene025665:intron 35.0%
AACCAAACAAACTTCATTGG+TGG - chr7.1:6455194-6455213 MS.gene025665:CDS 35.0%
AACCTACCAATATCACCAAT+TGG + chr7.1:6455233-6455252 None:intergenic 35.0%
AAGGAAGTCCTAAAAAACCA+AGG - chr7.1:6455045-6455064 MS.gene025665:CDS 35.0%
AATATACCCAACAATGGAGA+TGG + chr7.1:6453003-6453022 None:intergenic 35.0%
AATGTACGTGGAAGAAATGA+AGG - chr7.1:6454764-6454783 MS.gene025665:CDS 35.0%
AATGTGCACAAACCAAATTG+AGG - chr7.1:6453273-6453292 MS.gene025665:intron 35.0%
AATTCAGGATTCAGCTTCAT+TGG - chr7.1:6454996-6455015 MS.gene025665:CDS 35.0%
ACATGCTTGCAAAACAAACA+GGG - chr7.1:6454575-6454594 MS.gene025665:intron 35.0%
ACCTACCAATATCACCAATT+GGG + chr7.1:6455232-6455251 None:intergenic 35.0%
ACTCTTGTACTTAAGGAAAG+GGG - chr7.1:6452681-6452700 MS.gene025665:CDS 35.0%
AGAACAGAAACAGATCTTTC+AGG + chr7.1:6454225-6454244 None:intergenic 35.0%
ATAATAACAATGGGAATCCG+GGG - chr7.1:6451798-6451817 MS.gene025665:CDS 35.0%
ATATACTATTTGGACGTGCA+TGG - chr7.1:6453176-6453195 MS.gene025665:intron 35.0%
ATGAAATACCTCATCAAGCA+TGG + chr7.1:6452080-6452099 None:intergenic 35.0%
ATTGGATCATCAGAACTAGA+AGG - chr7.1:6455014-6455033 MS.gene025665:CDS 35.0%
CACTTCAAAGTTATGAAGCA+CGG - chr7.1:6454304-6454323 MS.gene025665:intron 35.0%
CATTGATGTTAAGCACAATG+AGG - chr7.1:6455100-6455119 MS.gene025665:CDS 35.0%
CCAAACCAAAACATTCAACT+AGG - chr7.1:6455347-6455366 MS.gene025665:CDS 35.0%
CCCGTGTGATTAATACAAAT+AGG + chr7.1:6453392-6453411 None:intergenic 35.0%
CTACTCTTGTACTTAAGGAA+AGG - chr7.1:6452679-6452698 MS.gene025665:CDS 35.0%
GAGGTAAAATTGAAGGTTCA+AGG - chr7.1:6454106-6454125 MS.gene025665:intron 35.0%
GATACTTTCATATTGAGGAG+AGG + chr7.1:6455423-6455442 None:intergenic 35.0%
GATGAGAGACAAAATAGAGA+TGG - chr7.1:6455155-6455174 MS.gene025665:CDS 35.0%
GCTCAAGATGTATAAATGCT+CGG - chr7.1:6454454-6454473 MS.gene025665:intron 35.0%
GGAAACCAAACAAACTTCAT+TGG - chr7.1:6455191-6455210 MS.gene025665:CDS 35.0%
GTGAATATTGGAGAATCATC+AGG - chr7.1:6451935-6451954 MS.gene025665:CDS 35.0%
GTTGAGGAAAACATAGTTAC+CGG + chr7.1:6451474-6451493 None:intergenic 35.0%
TAATGGCAGAGGTGATTAAA+TGG + chr7.1:6452940-6452959 None:intergenic 35.0%
TACTAGAGAGAAGAGAAAGT+TGG + chr7.1:6453134-6453153 None:intergenic 35.0%
TACTGGTATTATTGCTAGGT+AGG - chr7.1:6452855-6452874 MS.gene025665:CDS 35.0%
TAGGGTTATGCATAAGTTCA+TGG + chr7.1:6455073-6455092 None:intergenic 35.0%
TAGTCTAAATGGGTACATCA+AGG + chr7.1:6452898-6452917 None:intergenic 35.0%
TGAGATTGTTTGTAGTGCAA+GGG + chr7.1:6454004-6454023 None:intergenic 35.0%
TGAGCTAGCTCCAAATTAAT+GGG + chr7.1:6453067-6453086 None:intergenic 35.0%
TGTGTGGAATTGTAACCTTT+AGG + chr7.1:6454894-6454913 None:intergenic 35.0%
TTGAGATTGTTTGTAGTGCA+AGG + chr7.1:6454005-6454024 None:intergenic 35.0%
TTGCAAGGAATAGCATATAC+TGG - chr7.1:6452838-6452857 MS.gene025665:CDS 35.0%
TTGGATCATCAGAACTAGAA+GGG - chr7.1:6455015-6455034 MS.gene025665:CDS 35.0%
! ATACCAAATCTAAGGTCTAG+AGG - chr7.1:6453106-6453125 MS.gene025665:intron 35.0%
! ATCCACCAATGAAGTTTGTT+TGG + chr7.1:6455199-6455218 None:intergenic 35.0%
! ATGGATCAATAATTGGTGGT+GGG - chr7.1:6451975-6451994 MS.gene025665:CDS 35.0%
! ATGTTCAACAAGTGTTGTGT+TGG + chr7.1:6454419-6454438 None:intergenic 35.0%
! ATTTACTTGTTGGTTGAAGG+TGG - chr7.1:6453885-6453904 MS.gene025665:intron 35.0%
! CAATACCCAATTGGTGATAT+TGG - chr7.1:6455224-6455243 MS.gene025665:CDS 35.0%
! CTTACTCTTGTCCTTTGAAA+AGG - chr7.1:6453531-6453550 MS.gene025665:intron 35.0%
! CTTTTCAAAGGACAAGAGTA+AGG + chr7.1:6453533-6453552 None:intergenic 35.0%
! GCTAGAGATACTCTAAAGAA+AGG - chr7.1:6452960-6452979 MS.gene025665:intron 35.0%
! GTGAATAGTGGTGTTGAATT+GGG - chr7.1:6451884-6451903 MS.gene025665:CDS 35.0%
! TATTGGAGTTTCATGGCTTT+TGG + chr7.1:6454925-6454944 None:intergenic 35.0%
! TGTGAATAGTGGTGTTGAAT+TGG - chr7.1:6451883-6451902 MS.gene025665:CDS 35.0%
!! ACAAACTTCATTGGTGGATT+TGG - chr7.1:6455200-6455219 MS.gene025665:CDS 35.0%
!! ATTTGGTTTGGCCTTTCTAA+AGG - chr7.1:6452750-6452769 MS.gene025665:CDS 35.0%
!! CTCGGAGTCTTTAAAATCTT+CGG - chr7.1:6454472-6454491 MS.gene025665:intron 35.0%
!! TGCTTTGGAGGTAAAATTGA+AGG - chr7.1:6454099-6454118 MS.gene025665:intron 35.0%
!!! CCTAGTTGAATGTTTTGGTT+TGG + chr7.1:6455350-6455369 None:intergenic 35.0%
!!! TCATGGTTCCTTGGTTTTTT+AGG + chr7.1:6455056-6455075 None:intergenic 35.0%
AAACCACAACCTCCATTTGA+TGG + chr7.1:6453933-6453952 None:intergenic 40.0%
ACAACGTGCTCTACAACAAT+TGG - chr7.1:6454158-6454177 MS.gene025665:intron 40.0%
ACACTTGTTCTCATGAATCC+AGG - chr7.1:6451386-6451405 MS.gene025665:CDS 40.0%
ACGTACATTTCTTCCACCAT+TGG + chr7.1:6454753-6454772 None:intergenic 40.0%
ACTCAGAACAATTCACACCA+AGG - chr7.1:6455255-6455274 MS.gene025665:CDS 40.0%
AGATTCGTTTCTATGTCTGG+TGG - chr7.1:6451743-6451762 MS.gene025665:CDS 40.0%
ATCTTTCAGGCAATCCTCTT+TGG + chr7.1:6454212-6454231 None:intergenic 40.0%
ATGCTTTCTGAGCCTCAATT+TGG + chr7.1:6453288-6453307 None:intergenic 40.0%
ATGTGCTTGTCTGAATCCTT+TGG + chr7.1:6454559-6454578 None:intergenic 40.0%
ATTGGAGAATCATCAGGAGT+TGG - chr7.1:6451941-6451960 MS.gene025665:CDS 40.0%
CAAAACAAACAGGGCTTACT+AGG - chr7.1:6454584-6454603 MS.gene025665:intron 40.0%
CAACGTGCTCTACAACAATT+GGG - chr7.1:6454159-6454178 MS.gene025665:intron 40.0%
CACACATGCAGTACAATCAA+TGG - chr7.1:6451636-6451655 MS.gene025665:CDS 40.0%
CACATGCTTGCAAAACAAAC+AGG - chr7.1:6454574-6454593 MS.gene025665:intron 40.0%
CGTGGACCTTATAAATTGCA+AGG - chr7.1:6452823-6452842 MS.gene025665:CDS 40.0%
CTCTTGTACTTAAGGAAAGG+GGG - chr7.1:6452682-6452701 MS.gene025665:CDS 40.0%
GAAAGGGGGTTATGATAATC+TGG - chr7.1:6452696-6452715 MS.gene025665:CDS 40.0%
GAACACAATTGACCCAAACA+CGG - chr7.1:6451658-6451677 MS.gene025665:CDS 40.0%
GACATAGAAACGAATCTTGC+CGG + chr7.1:6451740-6451759 None:intergenic 40.0%
GAGCTAGCTCCAAATTAATG+GGG + chr7.1:6453066-6453085 None:intergenic 40.0%
GAGGTTGTGAATGTGAATAG+TGG - chr7.1:6451872-6451891 MS.gene025665:CDS 40.0%
GATAGAAAAACTGTGAGTGG+TGG + chr7.1:6452544-6452563 None:intergenic 40.0%
GATGGATCAATAATTGGTGG+TGG - chr7.1:6451974-6451993 MS.gene025665:CDS 40.0%
GCAAGATTCGTTTCTATGTC+TGG - chr7.1:6451740-6451759 MS.gene025665:CDS 40.0%
GCACAATGAGGCAAATAATG+AGG - chr7.1:6455112-6455131 MS.gene025665:CDS 40.0%
GCTAATTACCATGCTTGATG+AGG - chr7.1:6452069-6452088 MS.gene025665:CDS 40.0%
GGAATGAGCAAGGTGAATAT+TGG - chr7.1:6451923-6451942 MS.gene025665:CDS 40.0%
GGAGATGGATCAATAATTGG+TGG - chr7.1:6451971-6451990 MS.gene025665:CDS 40.0%
GGAGGAGATGGATCAATAAT+TGG - chr7.1:6451968-6451987 MS.gene025665:CDS 40.0%
GGATTTGGACAATACCCAAT+TGG - chr7.1:6455215-6455234 MS.gene025665:CDS 40.0%
GGCTTAGAGTCAACAATAGA+GGG - chr7.1:6453497-6453516 MS.gene025665:intron 40.0%
GGTTTATAAATGCTCGAGTC+AGG - chr7.1:6454718-6454737 MS.gene025665:intron 40.0%
GTAAAGGTCGTCAAACATAG+TGG - chr7.1:6453476-6453495 MS.gene025665:intron 40.0%
GTGAGCTAGCTCCAAATTAA+TGG + chr7.1:6453068-6453087 None:intergenic 40.0%
GTTGAATTGGGAAAGAAGAG+TGG - chr7.1:6451896-6451915 MS.gene025665:CDS 40.0%
TCAACATGCAAAACCCAAAG+AGG - chr7.1:6455441-6455460 MS.gene025665:CDS 40.0%
TCCAACACTCAACAATTCGT+TGG - chr7.1:6451539-6451558 MS.gene025665:CDS 40.0%
TCTCTCTAGTAGAGCCTATT+TGG - chr7.1:6453143-6453162 MS.gene025665:intron 40.0%
TCTCTGGTACCCCATTAATT+TGG - chr7.1:6453054-6453073 MS.gene025665:intron 40.0%
TCTTTCAGGCAATCCTCTTT+GGG + chr7.1:6454211-6454230 None:intergenic 40.0%
TGCATAAGTTCATGGTTCCT+TGG + chr7.1:6455065-6455084 None:intergenic 40.0%
TGGCTTAGAGTCAACAATAG+AGG - chr7.1:6453496-6453515 MS.gene025665:intron 40.0%
TTAGAGTCAACAATAGAGGG+TGG - chr7.1:6453500-6453519 MS.gene025665:intron 40.0%
! AACAAGTGTTTGAAGTCCGT+CGG + chr7.1:6451375-6451394 None:intergenic 40.0%
! AATGGTGGAAGAAATGTACG+TGG - chr7.1:6454752-6454771 MS.gene025665:CDS 40.0%
! ACCCAATTGGTGATATTGGT+AGG - chr7.1:6455228-6455247 MS.gene025665:CDS 40.0%
! ACCTTTAGGTTCTGTTTCAG+AGG + chr7.1:6454880-6454899 None:intergenic 40.0%
! CATTGATTGTACTGCATGTG+TGG + chr7.1:6451638-6451657 None:intergenic 40.0%
! TAGAAAAGTGTACTTACGGG+TGG + chr7.1:6454275-6454294 None:intergenic 40.0%
! TCTGAAATCCAATCAAGTGG+TGG - chr7.1:6451341-6451360 MS.gene025665:CDS 40.0%
!! AATTGTTGAGTGTTGGAAGG+TGG + chr7.1:6451536-6451555 None:intergenic 40.0%
!! ACCTCTGAAACAGAACCTAA+AGG - chr7.1:6454876-6454895 MS.gene025665:CDS 40.0%
!! ACGAATTGTTGAGTGTTGGA+AGG + chr7.1:6451539-6451558 None:intergenic 40.0%
!! AGCACATGAGCTTTTAGAAG+AGG - chr7.1:6451853-6451872 MS.gene025665:CDS 40.0%
!! GCTTTTGGAAATGTCTTGTG+TGG + chr7.1:6454910-6454929 None:intergenic 40.0%
!! TGGATCAATAATTGGTGGTG+GGG - chr7.1:6451976-6451995 MS.gene025665:CDS 40.0%
!! TTCCAAGGTGTTGAATTGGA+GGG + chr7.1:6452653-6452672 None:intergenic 40.0%
!! TTTCCAAGGTGTTGAATTGG+AGG + chr7.1:6452654-6452673 None:intergenic 40.0%
!!! GGTTTTTTAGGACTTCCTTG+TGG + chr7.1:6455044-6455063 None:intergenic 40.0%
AACTGTGCAGCAAACCTCTT+TGG + chr7.1:6455458-6455477 None:intergenic 45.0%
ACACATGCCATCTCCATTGT+TGG - chr7.1:6452993-6453012 MS.gene025665:intron 45.0%
ACAGTACCACCATCAAATGG+AGG - chr7.1:6453921-6453940 MS.gene025665:intron 45.0%
ACTGTGCAGCAAACCTCTTT+GGG + chr7.1:6455457-6455476 None:intergenic 45.0%
ATGGCAACGTACTATCATGG+TGG - chr7.1:6451314-6451333 MS.gene025665:CDS 45.0%
ATGGCAAGCACCTATCAAGA+AGG + chr7.1:6452921-6452940 None:intergenic 45.0%
CACATGCCATCTCCATTGTT+GGG - chr7.1:6452994-6453013 MS.gene025665:intron 45.0%
CAGACAGTACCACCATCAAA+TGG - chr7.1:6453918-6453937 MS.gene025665:intron 45.0%
CCACAATCATCACCACTTGT+TGG - chr7.1:6454960-6454979 MS.gene025665:CDS 45.0%
CTTTCAGGCAATCCTCTTTG+GGG + chr7.1:6454210-6454229 None:intergenic 45.0%
GAAAACATAGTTACCGGCAG+CGG + chr7.1:6451468-6451487 None:intergenic 45.0%
GAAATCCAATCAAGTGGTGG+CGG - chr7.1:6451344-6451363 MS.gene025665:CDS 45.0%
GAACTAGAAGGGATACCACA+AGG - chr7.1:6455026-6455045 MS.gene025665:CDS 45.0%
GAACTCAGAATCTGAATCCC+CGG + chr7.1:6451818-6451837 None:intergenic 45.0%
GAATGATCCAGCATAACGCT+TGG - chr7.1:6454181-6454200 MS.gene025665:intron 45.0%
GAATTGGGAAAGAAGAGTGG+TGG - chr7.1:6451899-6451918 MS.gene025665:CDS 45.0%
GACGTTGTACCTTCTTTGCA+TGG - chr7.1:6451605-6451624 MS.gene025665:CDS 45.0%
GATTGTACTGCATGTGTGGA+AGG + chr7.1:6451634-6451653 None:intergenic 45.0%
GCCAACGAATTGTTGAGTGT+TGG + chr7.1:6451543-6451562 None:intergenic 45.0%
GTATCTCTAGCTAATGGCAG+AGG + chr7.1:6452951-6452970 None:intergenic 45.0%
GTGCCTCTAGACCTTAGATT+TGG + chr7.1:6453112-6453131 None:intergenic 45.0%
TAAATGCTCGAGTCAGGCTT+TGG - chr7.1:6454724-6454743 MS.gene025665:CDS 45.0%
TCTTAGTCTCTCTTCACAGC+CGG - chr7.1:6451718-6451737 MS.gene025665:CDS 45.0%
TTCGTTGTAACACCAGAAGC+CGG + chr7.1:6451779-6451798 None:intergenic 45.0%
TTGGGTGAAGAGGATTGCTT+TGG - chr7.1:6454084-6454103 MS.gene025665:intron 45.0%
TTTCAGCAGCTGTAGAGTTG+AGG + chr7.1:6451490-6451509 None:intergenic 45.0%
! AAAAGTGTACTTACGGGTGG+AGG + chr7.1:6454272-6454291 None:intergenic 45.0%
! CCAACAAGTGGTGATGATTG+TGG + chr7.1:6454963-6454982 None:intergenic 45.0%
! GCAAAGAAGGTACAACGTCA+TGG + chr7.1:6451604-6451623 None:intergenic 45.0%
!! ATCAATAATTGGTGGTGGGG+AGG - chr7.1:6451979-6451998 MS.gene025665:CDS 45.0%
!! CTGTTTCTGTTCTTCGTGCT+TGG - chr7.1:6454232-6454251 MS.gene025665:intron 45.0%
!! GAATCATCAGGAGTTGGTAG+TGG - chr7.1:6451947-6451966 MS.gene025665:CDS 45.0%
!! GGTGAGAGCTTTTTCTTGAG+AGG + chr7.1:6454859-6454878 None:intergenic 45.0%
!! TCAATAATTGGTGGTGGGGA+GGG - chr7.1:6451980-6451999 MS.gene025665:CDS 45.0%
!! TCCAAGGTGTTGAATTGGAG+GGG + chr7.1:6452652-6452671 None:intergenic 45.0%
!! TGGTGTTGTTGGTGGTTAAG+AGG + chr7.1:6451569-6451588 None:intergenic 45.0%
!!! TTTTTATAGATTAATTTTTT+TGG - chr7.1:6452165-6452184 MS.gene025665:intron 5.0%
AACAGGGCTTACTAGGAGTC+AGG - chr7.1:6454591-6454610 MS.gene025665:intron 50.0%
CACAATTGACCCAAACACGG+CGG - chr7.1:6451661-6451680 MS.gene025665:CDS 50.0%
CACAGTCAATGGTACGTACG+TGG - chr7.1:6452805-6452824 MS.gene025665:CDS 50.0%
CCACAACCTCCATTTGATGG+TGG + chr7.1:6453930-6453949 None:intergenic 50.0%
CCACCATCAAATGGAGGTTG+TGG - chr7.1:6453927-6453946 MS.gene025665:intron 50.0%
GGAAGGAAGCCATGCAAAGA+AGG + chr7.1:6451617-6451636 None:intergenic 50.0%
GGTGAAGAGGATTGCTTTGG+AGG - chr7.1:6454087-6454106 MS.gene025665:intron 50.0%
GTTGGTTGAAGGTGGAGCAA+AGG - chr7.1:6453893-6453912 MS.gene025665:intron 50.0%
TTGGGAAAGAAGAGTGGTGG+TGG - chr7.1:6451902-6451921 MS.gene025665:CDS 50.0%
! TGTCCTAGCTGAACCGATTC+CGG + chr7.1:6453974-6453993 None:intergenic 50.0%
!! AGTCAGGCTTTGGAAGCCAA+TGG - chr7.1:6454734-6454753 MS.gene025665:CDS 50.0%
!! CGTGGATTTGGTGTTGTTGG+TGG + chr7.1:6451577-6451596 None:intergenic 50.0%
!! TCATCAGGAGTTGGTAGTGG+AGG - chr7.1:6451950-6451969 MS.gene025665:CDS 50.0%
AACGCTTGGAGACCCCAAAG+AGG - chr7.1:6454195-6454214 MS.gene025665:intron 55.0%
GATACACCACGCGCTCAACA+AGG - chr7.1:6451689-6451708 MS.gene025665:CDS 55.0%
GCCCCTCCAATTCAACACCT+TGG - chr7.1:6452648-6452667 MS.gene025665:CDS 55.0%
GTAACACCAGAAGCCGGAGA+CGG + chr7.1:6451773-6451792 None:intergenic 55.0%
TTCGAACAAGCCGCCGGAAT+CGG - chr7.1:6453958-6453977 MS.gene025665:intron 55.0%
!! ATCCAATCAAGTGGTGGCGG+CGG - chr7.1:6451347-6451366 MS.gene025665:CDS 55.0%
!! CAGGCTTTGGAAGCCAATGG+TGG - chr7.1:6454737-6454756 MS.gene025665:CDS 55.0%
!! GAGTGGTGGTGGAATGAGCA+AGG - chr7.1:6451913-6451932 MS.gene025665:CDS 55.0%
!! GCGCGTGGATTTGGTGTTGT+TGG + chr7.1:6451580-6451599 None:intergenic 55.0%
!! GGAGTTGGTAGTGGAGGAGA+TGG - chr7.1:6451956-6451975 MS.gene025665:CDS 55.0%
CAACCTTCTCATGCCGCTGC+CGG - chr7.1:6451452-6451471 MS.gene025665:CDS 60.0%
CGTCATGGTGCGCGTGGATT+TGG + chr7.1:6451589-6451608 None:intergenic 60.0%
GACAGTCCTTGTTGAGCGCG+TGG + chr7.1:6451698-6451717 None:intergenic 60.0%
GGGGTCTCCAAGCGTTATGC+TGG + chr7.1:6454191-6454210 None:intergenic 60.0%
GTACAACGTCATGGTGCGCG+TGG + chr7.1:6451595-6451614 None:intergenic 60.0%
TATCACGCGCCGCCGTGTTT+GGG + chr7.1:6451673-6451692 None:intergenic 60.0%
TCCTCGTTCGAACAAGCCGC+CGG - chr7.1:6453952-6453971 MS.gene025665:intron 60.0%
TTACCGGCAGCGGCATGAGA+AGG + chr7.1:6451458-6451477 None:intergenic 60.0%
! CCTAGCTGAACCGATTCCGG+CGG + chr7.1:6453971-6453990 None:intergenic 60.0%
!! ATTGGTGGTGGGGAGGGAAG+TGG - chr7.1:6451986-6452005 MS.gene025665:CDS 60.0%
AGGTGGCGCGTGAGGTGAGA+AGG + chr7.1:6451519-6451538 None:intergenic 65.0%
GTATCACGCGCCGCCGTGTT+TGG + chr7.1:6451674-6451693 None:intergenic 65.0%
GTCTGGTGGTTCGCCGTCTC+CGG - chr7.1:6451757-6451776 MS.gene025665:CDS 65.0%
GTGTTGGAAGGTGGCGCGTG+AGG + chr7.1:6451527-6451546 None:intergenic 65.0%
! CCGCCGGAATCGGTTCAGCT+AGG - chr7.1:6453968-6453987 MS.gene025665:intron 65.0%
! TCCGGCGGCTTGTTCGAACG+AGG + chr7.1:6453956-6453975 None:intergenic 65.0%
CGCCGCCGCCACCACTTGAT+TGG + chr7.1:6451352-6451371 None:intergenic 70.0%
GGTTCGCCGTCTCCGGCTTC+TGG - chr7.1:6451764-6451783 MS.gene025665:CDS 70.0%
!!! AGTGGTGGCGGCGGCGCCGA+CGG - chr7.1:6451356-6451375 MS.gene025665:CDS 80.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 6451314 6455499 6451314 ID=MS.gene025665
chr7.1 mRNA 6451314 6455499 6451314 ID=MS.gene025665.t1;Parent=MS.gene025665
chr7.1 exon 6454723 6455499 6454723 ID=MS.gene025665.t1.exon1;Parent=MS.gene025665.t1
chr7.1 CDS 6454723 6455499 6454723 ID=cds.MS.gene025665.t1;Parent=MS.gene025665.t1
chr7.1 exon 6452536 6452909 6452536 ID=MS.gene025665.t1.exon2;Parent=MS.gene025665.t1
chr7.1 CDS 6452536 6452909 6452536 ID=cds.MS.gene025665.t1;Parent=MS.gene025665.t1
chr7.1 exon 6452201 6452261 6452201 ID=MS.gene025665.t1.exon3;Parent=MS.gene025665.t1
chr7.1 CDS 6452201 6452261 6452201 ID=cds.MS.gene025665.t1;Parent=MS.gene025665.t1
chr7.1 exon 6451314 6452105 6451314 ID=MS.gene025665.t1.exon4;Parent=MS.gene025665.t1
chr7.1 CDS 6451314 6452105 6451314 ID=cds.MS.gene025665.t1;Parent=MS.gene025665.t1
Gene Sequence

>MS.gene025665

ATGGCAACGTACTATCATGGTGGTAATTCTGAAATCCAATCAAGTGGTGGCGGCGGCGCCGACGGACTTCAAACACTTGTTCTCATGAATCCAGGCTATATACAATATTCTGATGCTACACAGCAACAACAAGCTCAACAACCTTCTCATGCCGCTGCCGGTAACTATGTTTTCCTCAACTCTACAGCTGCTGAAAACAACTCCTTCTCACCTCACGCGCCACCTTCCAACACTCAACAATTCGTTGGCATACCTCTTAACCACCAACAACACCAAATCCACGCGCACCATGACGTTGTACCTTCTTTGCATGGCTTCCTTCCACACATGCAGTACAATCAATGGAACACAATTGACCCAAACACGGCGGCGCGTGATACACCACGCGCTCAACAAGGACTGTCTCTTAGTCTCTCTTCACAGCCGGCAAGATTCGTTTCTATGTCTGGTGGTTCGCCGTCTCCGGCTTCTGGTGTTACAACGAATAATAACAATGGGAATCCGGGGATTCAGATTCTGAGTTCAAAGTATTTAAGGGCAGCACATGAGCTTTTAGAAGAGGTTGTGAATGTGAATAGTGGTGTTGAATTGGGAAAGAAGAGTGGTGGTGGAATGAGCAAGGTGAATATTGGAGAATCATCAGGAGTTGGTAGTGGAGGAGATGGATCAATAATTGGTGGTGGGGAGGGAAGTGGAAAACGTAGCTCTGAGCTATCAACAGCTGAAAGACAAGAAATACAGATGAAGAAAGCTAAGCTAATTACCATGCTTGATGAGGTATTTCATTTCATTTCTTCACTTTCATGTGATTTTGGCTATTTGCAATAATTAATTAATTCAAACTATGATATTTTTTATAGATTAATTTTTTTGGTGAATGTATATAATTTTATTTTATAGATTATAATTATGTATTTTATAAGTTTAGTATAATTCTTGTTCAAAAAAAAAGTTTAGTATAATTTTTTGGCCATTTTAATTCATTATAAAAGTTAGAGACTAAAAATATATGTTTTTTGTAAAAGATAATGAATAAAAATTAATTAAATTTTTATAGGATTTAAAATTAGAAAATTGACATTTTGTAGTTTTACTAAAATTATATTTAACTTTTTATCTCTACGTGGAGAATTGTTTTTGCTAAAGTGGAGCAACTGAGCAAGATTGTTACAAGTATGATGTATACTGCTTTCATTGATTTATCATTGCAATGCAATGAATGAAAATCCACCACTCACAGTTTTTCTATCATTCGAGTATTTAAGCGGTTTCACTTGTACAAGTTATTTATTTTAACAATTTTTTTTTTAACTGGTGTATTCAATATAACTACTGCCCCTCCAATTCAACACCTTGGAAAAAAAACTACTCTTGTACTTAAGGAAAGGGGGTTATGATAATCTGGAATTTCTAACAATAACTTATAACTCAACATGATTTGGTTTGGCCTTTCTAAAGGAAAAACTCAACATCCTTACATTAATATTTTATCACAGTCAATGGTACGTACGTGGACCTTATAAATTGCAAGGAATAGCATATACTGGTATTATTGCTAGGTAGGCACCATTTATAATTTAACCTTGATGTACCCATTTAGACTACCTTCTTGATAGGTGCTTGCCATTTAATCACCTCTGCCATTAGCTAGAGATACTCTAAAGAAAGGTTTGTTAGGGACACATGCCATCTCCATTGTTGGGTATATTAGTCTTAGGTTTCTAATTAGGAATAGTTATTTCTCTGGTACCCCATTAATTTGGAGCTAGCTCACTTTGATGCGTACTTATTGATACCAAATCTAAGGTCTAGAGGCACCAACTTTCTCTTCTCTCTAGTAGAGCCTATTTGGTGTTTATGATATATACTATTTGGACGTGCATGGAGTTATTATTTGTGAGTCATTTAGTAAATTTTTTGGTGTTAGACCGGAAGAATGTGATATTGATTAGATATTTAAATGTGCACAAACCAAATTGAGGCTCAGAAAGCATAGAGTGAATATACTCTCAAATCTCAAAAGATAAAACTCAATTTTTAGCTTGAAACTTGGATATACATAGGTAGATATTGTCCCTATTTGTATTAATCACACGGGTCAATCTACAGTAGAAATAATATTATAGCAATAAATGACATAAATGGAAATACAATCTTTTAAAGTAAAGGTCGTCAAACATAGTGGCTTAGAGTCAACAATAGAGGGTGGTGCAAATCCTTACTCTTGTCCTTTGAAAAGGTGTAACTTAAGTAATTGTTGATATTAGTGAAATTTTAAATAGACTTAATAAAAACTTTTGATTCTATTAGTATTAGTAATTTTGATTGTGTCAATTTAGTTCATGAAAATAGAGACGAATACCATAAATCATTTCAATATTTTATTAATTTCAATAAAACTAATTGCATGGATTAATTAAATGAAATGAATTGTAAATATAAATTGACTTTACAAAGCCATCTAATGAAAATTCACTATTTTGTCACATGCCTCATTTTTATAATTACATTTTGAAATTAACGGTATGATATAAAAATAAAAGATTCTTGTTAAATGTCTGACTAAAATGAATTTACTTGTTGGTTGAAGGTGGAGCAAAGGTACAGACAGTACCACCATCAAATGGAGGTTGTGGTTTCCTCGTTCGAACAAGCCGCCGGAATCGGTTCAGCTAGGACATACACTGCCCTTGCACTACAAACAATCTCAAAGCAGTTTCGGTGTTTGAAAGATGCAATAACAGGACAAATTAAAGCTACAAATAAGAGTTTGGGTGAAGAGGATTGCTTTGGAGGTAAAATTGAAGGTTCAAGGCTCAAATATGTTGATCATCAACTACGTCAACAACGTGCTCTACAACAATTGGGAATGATCCAGCATAACGCTTGGAGACCCCAAAGAGGATTGCCTGAAAGATCTGTTTCTGTTCTTCGTGCTTGGCTCTTTGAACATTTCCTCCACCCGTAAGTACACTTTTCTAATTTTTACTCACTTCAAAGTTATGAAGCACGGACACTAATTACACGTTGATACCAATAAAGTGTTAGAGAAAATAACATAATTCAATGTAATCACAAGTGTTGATGTCGTCGTGTTTGTGTCCAACACAACACTTGTTGAACATCGTGACATGCCAAGTGCTCAAGATGTATAAATGCTCGGAGTCTTTAAAATCTTCGGTATATAGTCTTTAAACTCTTGTCAATTTTATGCTGATATCACTGAATTTGTTTTCAGCTATCCAAAGGATTCAGACAAGCACATGCTTGCAAAACAAACAGGGCTTACTAGGAGTCAGGTAATCAATCAATATAATTTTGGAACAATTTCTTTCCTTATTTTTGGCATATTCAAATTCAAAGTGTTAATAAAATTTTGTTTGAATTGCAACAGGTTTCAAATTGGTTTATAAATGCTCGAGTCAGGCTTTGGAAGCCAATGGTGGAAGAAATGTACGTGGAAGAAATGAAGGAAAATGAACTAAATGGATCTGAGGATAAACAAAGTAAGAGCAATGAAGATTCTTCAATGAAAATAACATCCTCTCAAGAAAAAGCTCTCACCTCTGAAACAGAACCTAAAGGTTACAATTCCACACAAGACATTTCCAAAAGCCATGAAACTCCAATAATTTCTGTTTCTAGACCACAATCATCACCACTTGTTGGAAATGTTAGAAACAATTCAGGATTCAGCTTCATTGGATCATCAGAACTAGAAGGGATACCACAAGGAAGTCCTAAAAAACCAAGGAACCATGAACTTATGCATAACCCTAATAGTTTCATTGATGTTAAGCACAATGAGGCAAATAATGAGGAACTATCAATGAAATTTGGTGATGAGAGACAAAATAGAGATGGTTACTCTTTTATGGGAAACCAAACAAACTTCATTGGTGGATTTGGACAATACCCAATTGGTGATATTGGTAGGTTTGACTCAGAACAATTCACACCAAGGTTTTCAAGTAATAATGTTTCACTCACTCTTGGTCTTGATTCAATACAAGGAAATCATCAAACATTTCTACCAAACCAAAACATTCAACTAGGAAGAAGCATTGATATAAGTGAACCAAATGAGTTTGGTTCTATAAACACTTCCTCTCCTCAATATGAAAGTATCAACATGCAAAACCCAAAGAGGTTTGCTGCACAGTTGTTACCAGATTTTGTGTCCTAA
Protein sequence

>MS.gene025665.t1

MATYYHGGNSEIQSSGGGGADGLQTLVLMNPGYIQYSDATQQQQAQQPSHAAAGNYVFLNSTAAENNSFSPHAPPSNTQQFVGIPLNHQQHQIHAHHDVVPSLHGFLPHMQYNQWNTIDPNTAARDTPRAQQGLSLSLSSQPARFVSMSGGSPSPASGVTTNNNNGNPGIQILSSKYLRAAHELLEEVVNVNSGVELGKKSGGGMSKVNIGESSGVGSGGDGSIIGGGEGSGKRSSELSTAERQEIQMKKAKLITMLDEVEQRYRQYHHQMEVVVSSFEQAAGIGSARTYTALALQTISKQFRCLKDAITGQIKATNKSLGEEDCFGGKIEGSRLKYVDHQLRQQRALQQLGMIQHNAWRPQRGLPERSVSVLRAWLFEHFLHPYPKDSDKHMLAKQTGLTRSQVSNWFINARVRLWKPMVEEMYVEEMKENELNGSEDKQSKSNEDSSMKITSSQEKALTSETEPKGYNSTQDISKSHETPIISVSRPQSSPLVGNVRNNSGFSFIGSSELEGIPQGSPKKPRNHELMHNPNSFIDVKHNEANNEELSMKFGDERQNRDGYSFMGNQTNFIGGFGQYPIGDIGRFDSEQFTPRFSSNNVSLTLGLDSIQGNHQTFLPNQNIQLGRSIDISEPNEFGSINTSSPQYESINMQNPKRFAAQLLPDFVS