Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025702.t1 | KEH19406.1 | 78.6 | 103 | 20 | 1 | 8 | 110 | 41 | 141 | 2.30E-39 | 171.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025702.t1 | Q9FNG6 | 48.3 | 151 | 28 | 1 | 4 | 104 | 128 | 278 | 2.0e-31 | 136.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025702.t1 | A0A072TQE4 | 78.6 | 103 | 20 | 1 | 8 | 110 | 41 | 141 | 1.7e-39 | 171.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene025702.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025702.t1 | MTR_8g058607 | 78.641 | 103 | 20 | 1 | 8 | 110 | 41 | 141 | 4.77e-56 | 170 |
| MS.gene025702.t1 | MTR_7g108868 | 78.218 | 101 | 20 | 1 | 8 | 108 | 28 | 126 | 8.97e-55 | 169 |
| MS.gene025702.t1 | MTR_8g058593 | 78.218 | 101 | 20 | 1 | 8 | 108 | 28 | 126 | 8.97e-55 | 169 |
| MS.gene025702.t1 | MTR_7g109250 | 50.658 | 152 | 25 | 1 | 7 | 108 | 86 | 237 | 2.32e-45 | 149 |
| MS.gene025702.t1 | MTR_7g109250 | 50.658 | 152 | 25 | 1 | 7 | 108 | 146 | 297 | 3.60e-44 | 147 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025702.t1 | AT5G06420 | 49.045 | 157 | 30 | 1 | 4 | 110 | 128 | 284 | 1.19e-42 | 143 |
| MS.gene025702.t1 | AT5G06420 | 49.045 | 157 | 30 | 1 | 4 | 110 | 128 | 284 | 1.19e-42 | 143 |
| MS.gene025702.t1 | AT1G01350 | 48.408 | 157 | 31 | 1 | 4 | 110 | 93 | 249 | 1.31e-41 | 140 |
Find 28 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGACTCAAAATGAAAGT+TGG | 0.376024 | 7.4:-6352646 | None:intergenic |
| AAAATGAAAGTTGGGCTTCT+CGG | 0.382812 | 7.4:-6352637 | None:intergenic |
| TTGAAGTCTTTGCATATGTC+AGG | 0.387439 | 7.4:-6352817 | None:intergenic |
| GAAGACTCAAAATGAAAGTT+GGG | 0.391037 | 7.4:-6352645 | None:intergenic |
| GACCGAGGCGACTACAATTC+CGG | 0.402115 | 7.4:+6352886 | MS.gene025702:CDS |
| TGACATATGCAAAGACTTCA+AGG | 0.411330 | 7.4:+6352819 | MS.gene025702:CDS |
| TCAGCGCACCCTCCTCTTCT+CGG | 0.422760 | 7.4:-6352938 | None:intergenic |
| TAGCAAAGCAACAGCAACTT+TGG | 0.436558 | 7.4:+6352693 | MS.gene025702:CDS |
| TGCAAAGACTTCAAGGAGAC+CGG | 0.477457 | 7.4:+6352826 | MS.gene025702:CDS |
| TTCTTTGTCCAACTGCCATC+CGG | 0.488671 | 7.4:-6352905 | None:intergenic |
| GATGGCAGTTGGACAAAGAA+TGG | 0.494461 | 7.4:+6352908 | MS.gene025702:CDS |
| TTCAAGGAGACCGGGTATTG+TGG | 0.545327 | 7.4:+6352835 | MS.gene025702:CDS |
| AGAAGAGGAGGGTGCGCTGA+TGG | 0.548558 | 7.4:+6352941 | MS.gene025702:CDS |
| CTACAATTCCGGATGGCAGT+TGG | 0.553003 | 7.4:+6352897 | MS.gene025702:CDS |
| ATCCGGAATTGTAGTCGCCT+CGG | 0.556890 | 7.4:-6352888 | None:intergenic |
| GGAATGATGCCGAGAAGAGG+AGG | 0.558808 | 7.4:+6352929 | MS.gene025702:CDS |
| TGCAAGTTTCTTCACGACCG+AGG | 0.564969 | 7.4:+6352871 | MS.gene025702:CDS |
| GAGACCGGGTATTGTGGCTA+CGG | 0.578795 | 7.4:+6352841 | MS.gene025702:CDS |
| ATCGCCGTAGCCACAATACC+CGG | 0.586901 | 7.4:-6352845 | None:intergenic |
| GAAACTCTAATATGAGTCGA+AGG | 0.595574 | 7.4:-6352775 | None:intergenic |
| AATGGAATGATGCCGAGAAG+AGG | 0.596970 | 7.4:+6352926 | MS.gene025702:CDS |
| GCAAAGACTTCAAGGAGACC+GGG | 0.633254 | 7.4:+6352827 | MS.gene025702:CDS |
| GAATGATGCCGAGAAGAGGA+GGG | 0.646762 | 7.4:+6352930 | MS.gene025702:CDS |
| ATCCGTGGGTCATACTCACA+CGG | 0.649413 | 7.4:+6352742 | MS.gene025702:CDS |
| GTGTGAGTATGACCCACGGA+TGG | 0.652577 | 7.4:-6352740 | None:intergenic |
| GACCGTGTGAGTATGACCCA+CGG | 0.674066 | 7.4:-6352744 | None:intergenic |
| GAGGCGACTACAATTCCGGA+TGG | 0.682453 | 7.4:+6352890 | MS.gene025702:CDS |
| ATATGAGTCGAAGGTCTGAG+AGG | 0.697705 | 7.4:-6352766 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAAGACTCAAAATGAAAGTT+GGG | - | chr7.4:6352648-6352667 | None:intergenic | 30.0% | |
| TGAAGACTCAAAATGAAAGT+TGG | - | chr7.4:6352649-6352668 | None:intergenic | 30.0% | |
| AAAATGAAAGTTGGGCTTCT+CGG | - | chr7.4:6352640-6352659 | None:intergenic | 35.0% | |
| GAAACTCTAATATGAGTCGA+AGG | - | chr7.4:6352778-6352797 | None:intergenic | 35.0% | |
| TGACATATGCAAAGACTTCA+AGG | + | chr7.4:6352819-6352838 | MS.gene025702:CDS | 35.0% | |
| TTGAAGTCTTTGCATATGTC+AGG | - | chr7.4:6352820-6352839 | None:intergenic | 35.0% | |
| !!! | ATTTTTCAAGTTCCATCCGT+GGG | + | chr7.4:6352728-6352747 | MS.gene025702:CDS | 35.0% |
| TAGCAAAGCAACAGCAACTT+TGG | + | chr7.4:6352693-6352712 | MS.gene025702:CDS | 40.0% | |
| !!! | CATTTTTCAAGTTCCATCCG+TGG | + | chr7.4:6352727-6352746 | MS.gene025702:CDS | 40.0% |
| AATGGAATGATGCCGAGAAG+AGG | + | chr7.4:6352926-6352945 | MS.gene025702:CDS | 45.0% | |
| ATATGAGTCGAAGGTCTGAG+AGG | - | chr7.4:6352769-6352788 | None:intergenic | 45.0% | |
| GATGGCAGTTGGACAAAGAA+TGG | + | chr7.4:6352908-6352927 | MS.gene025702:CDS | 45.0% | |
| TGCAAAGACTTCAAGGAGAC+CGG | + | chr7.4:6352826-6352845 | MS.gene025702:CDS | 45.0% | |
| TTCTTTGTCCAACTGCCATC+CGG | - | chr7.4:6352908-6352927 | None:intergenic | 45.0% | |
| ATCCGGAATTGTAGTCGCCT+CGG | - | chr7.4:6352891-6352910 | None:intergenic | 50.0% | |
| ATCCGTGGGTCATACTCACA+CGG | + | chr7.4:6352742-6352761 | MS.gene025702:CDS | 50.0% | |
| CTACAATTCCGGATGGCAGT+TGG | + | chr7.4:6352897-6352916 | MS.gene025702:CDS | 50.0% | |
| GAATGATGCCGAGAAGAGGA+GGG | + | chr7.4:6352930-6352949 | MS.gene025702:CDS | 50.0% | |
| GCAAAGACTTCAAGGAGACC+GGG | + | chr7.4:6352827-6352846 | MS.gene025702:CDS | 50.0% | |
| TGCAAGTTTCTTCACGACCG+AGG | + | chr7.4:6352871-6352890 | MS.gene025702:CDS | 50.0% | |
| ! | TTCAAGGAGACCGGGTATTG+TGG | + | chr7.4:6352835-6352854 | MS.gene025702:CDS | 50.0% |
| ATCGCCGTAGCCACAATACC+CGG | - | chr7.4:6352848-6352867 | None:intergenic | 55.0% | |
| GACCGAGGCGACTACAATTC+CGG | + | chr7.4:6352886-6352905 | MS.gene025702:CDS | 55.0% | |
| GACCGTGTGAGTATGACCCA+CGG | - | chr7.4:6352747-6352766 | None:intergenic | 55.0% | |
| GAGGCGACTACAATTCCGGA+TGG | + | chr7.4:6352890-6352909 | MS.gene025702:CDS | 55.0% | |
| GGAATGATGCCGAGAAGAGG+AGG | + | chr7.4:6352929-6352948 | MS.gene025702:CDS | 55.0% | |
| GTGTGAGTATGACCCACGGA+TGG | - | chr7.4:6352743-6352762 | None:intergenic | 55.0% | |
| ! | GAGACCGGGTATTGTGGCTA+CGG | + | chr7.4:6352841-6352860 | MS.gene025702:CDS | 55.0% |
| TCAGCGCACCCTCCTCTTCT+CGG | - | chr7.4:6352941-6352960 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 6352628 | 6352960 | 6352628 | ID=MS.gene025702 |
| chr7.4 | mRNA | 6352628 | 6352960 | 6352628 | ID=MS.gene025702.t1;Parent=MS.gene025702 |
| chr7.4 | exon | 6352628 | 6352960 | 6352628 | ID=MS.gene025702.t1.exon1;Parent=MS.gene025702.t1 |
| chr7.4 | CDS | 6352628 | 6352960 | 6352628 | ID=cds.MS.gene025702.t1;Parent=MS.gene025702.t1 |
| Gene Sequence |
| Protein sequence |
