AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025890.t1 MTR_7g117705 81.967 488 35 7 1 462 1 461 0.0 739
MS.gene025890.t1 MTR_1g013180 65.019 526 118 11 1 461 152 676 0.0 608
MS.gene025890.t1 MTR_1g013170 61.807 487 108 9 1 410 1 486 0.0 516
MS.gene025890.t1 MTR_3g106220 63.950 319 103 4 3 316 6 317 1.98e-128 386
MS.gene025890.t1 MTR_2g450070 47.570 391 163 11 3 364 16 393 5.15e-98 308
MS.gene025890.t1 MTR_2g450070 47.570 391 163 11 3 364 16 393 8.38e-98 308
MS.gene025890.t1 MTR_2g034960 52.249 289 128 3 1 282 25 310 1.09e-89 285
MS.gene025890.t1 MTR_4g131580 48.083 339 166 5 5 343 17 345 4.06e-89 283
MS.gene025890.t1 MTR_4g131600 47.273 330 159 6 1 323 16 337 2.15e-86 276
MS.gene025890.t1 MTR_4g131570 44.598 361 168 8 3 348 15 358 2.60e-86 276
MS.gene025890.t1 MTR_4g121020 55.385 260 109 2 2 258 28 283 1.13e-85 276
MS.gene025890.t1 MTR_4g121020 55.385 260 109 2 2 258 28 283 1.53e-85 276
MS.gene025890.t1 MTR_1g032570 50.909 275 116 4 4 265 10 278 4.22e-84 271
MS.gene025890.t1 MTR_3g086100 51.866 268 118 3 5 266 13 275 2.18e-82 265
MS.gene025890.t1 MTR_8g079940 51.812 276 108 5 3 266 23 285 1.10e-80 259
MS.gene025890.t1 MTR_1g013160 82.550 149 25 1 67 214 1 149 3.82e-72 225
MS.gene025890.t1 MTR_3g102590 51.538 260 72 3 2 258 5 213 4.79e-72 228
MS.gene025890.t1 MTR_3g102590 51.464 239 64 2 2 239 5 192 5.63e-65 208
MS.gene025890.t1 MTR_1g032570 50.909 220 79 7 64 265 1 209 1.33e-57 199
MS.gene025890.t1 MTR_3g086100 49.761 209 94 3 64 266 1 204 1.91e-55 192
MS.gene025890.t1 MTR_4g021760 39.506 243 120 4 5 247 11 226 4.05e-52 178
MS.gene025890.t1 MTR_3g102600 68.217 129 41 0 2 130 5 133 3.55e-51 172
MS.gene025890.t1 MTR_3g102600 62.264 53 20 0 189 241 131 183 7.72e-17 79.0
MS.gene025890.t1 MTR_3g102600 67.692 130 42 0 1 130 4 133 8.10e-51 172
MS.gene025890.t1 MTR_3g102600 45.902 122 33 4 189 309 131 220 1.09e-22 96.7
MS.gene025890.t1 MTR_4g021790 38.776 245 123 4 2 246 7 224 1.14e-48 168
MS.gene025890.t1 MTR_4g021845 37.815 238 121 4 2 239 7 217 1.77e-46 160
MS.gene025890.t1 MTR_4g021855 37.354 257 133 5 2 258 7 235 2.75e-46 162
MS.gene025890.t1 MTR_0450s0040 36.502 263 138 5 9 265 8 247 6.66e-46 163
MS.gene025890.t1 MTR_7g026400 37.238 239 128 4 32 265 14 235 9.25e-41 154
MS.gene025890.t1 MTR_4g121020 47.753 178 86 2 84 258 6 179 1.23e-39 150
MS.gene025890.t1 MTR_8g105600 31.922 307 191 6 5 301 9 307 7.31e-39 148
MS.gene025890.t1 MTR_6g045327 35.656 244 128 5 4 246 13 228 2.90e-37 140
MS.gene025890.t1 MTR_3g102600 67.857 84 27 0 47 130 1 84 5.31e-33 122
MS.gene025890.t1 MTR_3g102600 62.264 53 20 0 189 241 82 134 3.25e-17 78.6
MS.gene025890.t1 MTR_3g102600 67.857 84 27 0 47 130 1 84 2.08e-32 122
MS.gene025890.t1 MTR_3g102600 45.902 122 33 4 189 309 82 171 6.77e-23 95.9
MS.gene025890.t1 MTR_5g055260 32.653 245 111 6 2 246 7 197 3.05e-30 118
MS.gene025890.t1 MTR_8g032710 27.899 276 174 7 8 262 20 291 1.69e-27 115
MS.gene025890.t1 MTR_4g064730 47.788 113 56 2 164 274 103 214 1.22e-23 101
MS.gene025890.t1 MTR_6g016850 34.646 127 80 2 3 127 7 132 1.25e-21 92.0
MS.gene025890.t1 MTR_3g037390 31.928 166 106 2 9 171 24 185 5.00e-21 96.3
MS.gene025890.t1 MTR_4g023980 30.723 166 89 3 51 216 35 174 9.09e-20 87.0
MS.gene025890.t1 MTR_8g024260 32.738 168 99 3 11 164 38 205 1.00e-19 92.4
MS.gene025890.t1 MTR_3g092780 32.374 139 89 2 9 143 56 193 1.43e-19 92.0
MS.gene025890.t1 MTR_3g092780 32.374 139 89 2 9 143 56 193 1.55e-19 92.0
MS.gene025890.t1 MTR_3g092780 34.646 127 78 2 9 131 56 181 2.34e-19 91.3
MS.gene025890.t1 MTR_3g092780 34.646 127 78 2 9 131 56 181 2.73e-19 91.3
MS.gene025890.t1 MTR_7g089010 63.793 58 21 0 189 246 90 147 3.32e-19 88.2
MS.gene025890.t1 MTR_8g093040 35.484 124 80 0 4 127 13 136 3.71e-19 87.8
MS.gene025890.t1 MTR_7g118260 31.053 190 105 5 2 182 5 177 5.64e-19 90.1
MS.gene025890.t1 MTR_4g108880 29.518 166 110 2 9 171 33 194 3.38e-18 87.8
MS.gene025890.t1 MTR_7g118260 34.307 137 79 3 2 130 5 138 8.75e-18 86.3
MS.gene025890.t1 MTR_1g067110 33.607 122 78 2 9 127 85 206 9.58e-17 83.6
MS.gene025890.t1 MTR_1g067110 33.607 122 78 2 9 127 85 206 1.06e-16 83.2
MS.gene025890.t1 MTR_2g090675 38.053 113 65 2 183 293 154 263 1.24e-16 82.0
MS.gene025890.t1 MTR_4g061360 35.246 122 76 1 9 127 89 210 2.57e-16 82.0
MS.gene025890.t1 MTR_6g453220 54.762 84 32 1 207 284 380 463 4.74e-16 80.9
MS.gene025890.t1 MTR_4g078810 43.590 78 44 0 185 262 153 230 5.00e-16 80.5
MS.gene025890.t1 MTR_5g014040 59.016 61 25 0 188 248 309 369 1.10e-15 79.7
MS.gene025890.t1 MTR_5g014040 34.400 125 76 3 5 127 14 134 1.17e-11 67.0
MS.gene025890.t1 MTR_4g098870 57.377 61 26 0 188 248 308 368 5.03e-15 77.8
MS.gene025890.t1 MTR_4g098870 32.258 124 80 2 5 127 14 134 3.72e-11 65.5
MS.gene025890.t1 MTR_4g098870 57.377 61 26 0 188 248 328 388 5.16e-15 77.8
MS.gene025890.t1 MTR_4g098870 32.258 124 80 2 5 127 34 154 6.80e-11 64.7
MS.gene025890.t1 MTR_4g098870 57.377 61 26 0 188 248 328 388 5.16e-15 77.8
MS.gene025890.t1 MTR_4g098870 32.258 124 80 2 5 127 34 154 4.48e-11 65.5
MS.gene025890.t1 MTR_4g098870 57.377 61 26 0 188 248 308 368 5.50e-15 77.8
MS.gene025890.t1 MTR_4g098870 32.258 124 80 2 5 127 14 134 6.46e-11 64.7
MS.gene025890.t1 MTR_4g098870 57.377 61 26 0 188 248 347 407 5.61e-15 77.8
MS.gene025890.t1 MTR_4g098870 32.258 124 80 2 5 127 53 173 4.96e-11 65.5
MS.gene025890.t1 MTR_1g093080 45.238 84 45 1 176 258 197 280 7.39e-15 76.3
MS.gene025890.t1 MTR_5g014040 54.545 66 25 1 188 248 269 334 8.79e-14 73.6
MS.gene025890.t1 MTR_5g014040 54.545 66 25 1 188 248 309 374 1.13e-13 73.6
MS.gene025890.t1 MTR_5g014040 34.400 125 76 3 5 127 14 134 1.33e-11 67.0
MS.gene025890.t1 MTR_4g106590 33.588 131 74 2 9 126 26 156 1.63e-13 70.1
MS.gene025890.t1 MTR_5g017980 46.479 71 38 0 187 257 189 259 9.11e-13 69.3
MS.gene025890.t1 MTR_5g017980 46.479 71 38 0 187 257 190 260 9.70e-13 69.3
MS.gene025890.t1 MTR_5g054300 51.562 64 30 1 184 246 192 255 2.17e-12 68.6
MS.gene025890.t1 MTR_7g115530 48.193 83 38 2 188 269 216 294 2.22e-12 68.2
MS.gene025890.t1 MTR_7g115530 48.193 83 38 2 188 269 216 294 3.63e-12 67.8
MS.gene025890.t1 MTR_4g086835 48.529 68 33 1 189 254 273 340 3.67e-12 68.2
MS.gene025890.t1 MTR_7g115530 48.193 83 38 2 188 269 216 294 4.21e-12 68.2
MS.gene025890.t1 MTR_7g115530 52.778 72 33 1 188 258 216 287 5.52e-12 67.4
MS.gene025890.t1 MTR_8g038620 30.435 138 76 3 11 128 11 148 5.54e-12 64.7
MS.gene025890.t1 MTR_1g080330 56.897 58 24 1 186 242 171 228 6.07e-12 66.6
MS.gene025890.t1 MTR_6g088950 29.032 124 87 1 7 129 5 128 6.75e-12 65.1
MS.gene025890.t1 MTR_1g080330 56.897 58 24 1 186 242 171 228 8.69e-12 67.0
MS.gene025890.t1 MTR_2g027800 50.794 63 30 1 186 247 35 97 1.45e-11 65.5
MS.gene025890.t1 MTR_1g053830 46.269 67 33 2 192 255 20 86 1.56e-11 61.2
MS.gene025890.t1 MTR_5g017980 47.143 70 36 1 189 257 192 261 2.23e-11 65.5
MS.gene025890.t1 MTR_4g113140 33.333 126 58 2 122 246 192 292 3.87e-11 65.1
MS.gene025890.t1 MTR_4g086835 47.826 69 33 2 189 254 273 341 6.49e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025890.t1 AT2G25180 58.466 313 120 6 3 310 11 318 1.02e-107 332
MS.gene025890.t1 AT4G31920 52.601 346 130 7 3 333 11 337 2.63e-104 321
MS.gene025890.t1 AT3G16857 43.229 384 153 10 3 351 31 384 9.88e-86 276
MS.gene025890.t1 AT3G16857 43.229 384 153 10 3 351 31 384 1.95e-85 276
MS.gene025890.t1 AT5G58080 50.152 329 123 10 3 313 12 317 1.20e-83 270
MS.gene025890.t1 AT2G01760 47.423 291 139 5 1 283 3 287 1.53e-79 252
MS.gene025890.t1 AT1G67710 52.964 253 109 4 6 252 8 256 2.10e-77 251
MS.gene025890.t1 AT2G01760 43.260 319 139 6 1 283 3 315 2.11e-73 237
MS.gene025890.t1 AT3G16857 42.274 343 133 11 44 351 2 314 4.59e-68 229
MS.gene025890.t1 AT2G01760 45.175 228 111 5 64 283 1 222 1.43e-51 177
MS.gene025890.t1 AT1G67710 51.282 195 85 4 64 252 1 191 2.89e-50 177
MS.gene025890.t1 AT5G49240 37.815 238 142 3 5 239 38 272 4.50e-47 164
MS.gene025890.t1 AT3G62670 39.095 243 122 8 10 242 42 268 1.44e-42 156
MS.gene025890.t1 AT1G49190 30.796 289 152 6 4 266 29 295 1.42e-36 139
MS.gene025890.t1 AT1G67710 45.714 175 85 4 84 252 12 182 6.41e-36 138
MS.gene025890.t1 AT5G07210 29.562 274 156 7 7 249 14 281 1.45e-31 128
MS.gene025890.t1 AT5G07210 28.723 282 156 8 7 249 14 289 4.50e-29 120
MS.gene025890.t1 AT2G27070 28.155 309 153 8 7 271 14 297 1.99e-28 118
MS.gene025890.t1 AT3G46640 39.333 150 86 3 177 324 129 275 1.25e-23 101
MS.gene025890.t1 AT3G46640 39.333 150 86 3 177 324 129 275 2.31e-23 100
MS.gene025890.t1 AT3G46640 39.333 150 86 3 177 324 129 275 2.31e-23 100
MS.gene025890.t1 AT5G59570 50.000 98 47 1 170 265 121 218 8.12e-23 98.6
MS.gene025890.t1 AT5G59570 50.000 98 47 1 170 265 121 218 8.12e-23 98.6
MS.gene025890.t1 AT1G49190 29.442 197 117 3 4 200 29 203 3.85e-22 100
MS.gene025890.t1 AT2G46790 37.398 123 72 2 9 127 37 158 1.79e-21 97.1
MS.gene025890.t1 AT2G46790 37.398 123 72 2 9 127 37 158 2.10e-21 97.1
MS.gene025890.t1 AT5G05090 46.000 100 53 1 175 273 65 164 3.10e-20 90.5
MS.gene025890.t1 AT2G40970 63.793 58 21 0 189 246 104 161 6.40e-20 89.4
MS.gene025890.t1 AT3G10760 63.793 58 21 0 189 246 104 161 1.39e-19 90.1
MS.gene025890.t1 AT5G61380 30.723 166 108 2 9 171 19 180 2.35e-19 91.3
MS.gene025890.t1 AT5G24470 34.375 128 79 2 7 130 48 174 3.44e-19 90.9
MS.gene025890.t1 AT5G44190 46.512 86 46 0 182 267 139 224 3.25e-18 86.3
MS.gene025890.t1 AT5G44190 48.750 80 41 0 188 267 145 224 4.01e-18 86.3
MS.gene025890.t1 AT5G60100 34.646 127 79 2 5 127 59 185 1.58e-17 84.7
MS.gene025890.t1 AT5G60100 34.646 127 79 2 5 127 59 185 1.58e-17 84.7
MS.gene025890.t1 AT5G60100 34.646 127 79 2 5 127 59 185 1.58e-17 84.7
MS.gene025890.t1 AT5G60100 34.646 127 79 2 5 127 59 185 2.41e-17 84.7
MS.gene025890.t1 AT5G60100 34.646 127 79 2 5 127 59 185 2.41e-17 84.7
MS.gene025890.t1 AT5G60100 34.646 127 79 2 5 127 59 185 2.41e-17 84.7
MS.gene025890.t1 AT5G60100 34.646 127 79 2 5 127 59 185 3.88e-17 84.3
MS.gene025890.t1 AT5G02810 33.607 122 78 1 9 127 78 199 1.30e-16 83.2
MS.gene025890.t1 AT2G20570 48.684 76 39 0 187 262 150 225 2.75e-16 81.3
MS.gene025890.t1 AT2G20570 50.704 71 35 0 192 262 171 241 1.37e-15 79.0
MS.gene025890.t1 AT4G18020 37.398 123 57 2 164 286 272 374 6.09e-14 74.3
MS.gene025890.t1 AT4G18020 37.398 123 57 2 164 286 272 374 6.09e-14 74.3
MS.gene025890.t1 AT4G18020 37.398 123 57 2 164 286 272 374 6.09e-14 74.3
MS.gene025890.t1 AT4G18020 37.398 123 57 2 164 286 272 374 6.09e-14 74.3
MS.gene025890.t1 AT4G18020 37.398 123 57 2 164 286 272 374 6.09e-14 74.3
MS.gene025890.t1 AT4G18020 37.398 123 57 2 164 286 272 374 7.26e-14 73.9
MS.gene025890.t1 AT4G18020 37.398 123 57 2 164 286 272 374 7.26e-14 73.9
MS.gene025890.t1 AT4G18020 37.398 123 57 2 164 286 272 374 7.26e-14 73.9
MS.gene025890.t1 AT4G18020 37.398 123 57 2 164 286 272 374 7.26e-14 73.9
MS.gene025890.t1 AT4G18020 37.398 123 57 2 164 286 272 374 7.26e-14 73.9
MS.gene025890.t1 AT4G18020 37.398 123 57 2 164 286 272 374 7.26e-14 73.9
MS.gene025890.t1 AT3G57040 28.834 163 94 2 11 151 11 173 8.45e-13 68.2
MS.gene025890.t1 AT3G57040 28.834 163 94 2 11 151 11 173 8.45e-13 68.2
MS.gene025890.t1 AT3G04030 42.683 82 42 1 190 271 45 121 6.62e-12 67.4
MS.gene025890.t1 AT5G18240 40.566 106 54 3 190 294 45 142 7.60e-12 67.4
MS.gene025890.t1 AT5G18240 40.566 106 54 3 190 294 45 142 7.60e-12 67.4
MS.gene025890.t1 AT5G18240 40.566 106 54 3 190 294 45 142 8.67e-12 67.0
MS.gene025890.t1 AT5G18240 40.566 106 54 3 190 294 45 142 8.67e-12 67.0
MS.gene025890.t1 AT5G18240 40.566 106 54 3 190 294 45 142 8.99e-12 67.0
MS.gene025890.t1 AT1G25550 47.297 74 32 2 174 246 199 266 1.55e-11 65.9
MS.gene025890.t1 AT3G04030 43.373 83 41 2 190 271 45 122 2.23e-11 65.9
MS.gene025890.t1 AT3G04030 43.373 83 41 2 190 271 45 122 2.39e-11 65.9
MS.gene025890.t1 AT2G03500 52.542 59 27 1 189 246 232 290 4.25e-11 65.1
MS.gene025890.t1 AT1G10470 30.282 142 87 2 11 140 22 163 8.83e-11 62.4

Find 87 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCTCTTTACATGGAAAA+AGG 0.209902 7.1:+678737 MS.gene025890:CDS
GGAAGGTAAGAATTTGTTTC+AGG 0.212805 7.1:-678793 None:intergenic
CTTGCTGTTGTTAATCAATT+AGG 0.232624 7.1:+677730 MS.gene025890:CDS
TACCAATCACATGATTATTA+TGG 0.258074 7.1:-678873 None:intergenic
TAGAACTTGTCTCAAGGTTT+TGG 0.268683 7.1:+676305 MS.gene025890:CDS
TGATTTGGCAACATGTAATT+AGG 0.275585 7.1:+677476 MS.gene025890:CDS
CCATTTCCACTATCAAAATT+AGG 0.281786 7.1:-677535 None:intergenic
TATCTGAAAGGAATTAGTTC+TGG 0.286831 7.1:+678445 MS.gene025890:CDS
ATGAATGATCAGTTTCCTAT+TGG 0.311031 7.1:+676255 MS.gene025890:CDS
ATGAAAAGCCAACTAGAAAA+AGG 0.314880 7.1:+677593 MS.gene025890:CDS
GCCTCATGTGGTTTCTTTGA+AGG 0.325335 7.1:-678946 None:intergenic
CATAAATTATATTACCTGTT+TGG 0.347407 7.1:-679055 None:intergenic
TGAAAGAGGTACAGATGATT+TGG 0.347739 7.1:+677461 MS.gene025890:CDS
CATGTGATTGGTAGTTCAAT+AGG 0.356360 7.1:+678883 MS.gene025890:CDS
GGCATTCTTTGAACATGGTT+CGG 0.368479 7.1:-678679 None:intergenic
GTTCGGATTGGCGTGAGCTT+TGG 0.373257 7.1:-678662 None:intergenic
TTTAAGCTTCTTGAGCTTGT+TGG 0.375182 7.1:+677245 MS.gene025890:CDS
TGAGAGGCTACAGAAGAAAT+TGG 0.392769 7.1:-678700 None:intergenic
AATGGGATGTATTTGCTTGT+TGG 0.392821 7.1:-678474 None:intergenic
AACCATAATAATCATGTGAT+TGG 0.416988 7.1:+678871 MS.gene025890:CDS
ATTCCTTTCATCACCATCCT+TGG 0.417097 7.1:-677397 None:intergenic
AGAAGAAGCCTCGAGTTGTT+TGG 0.418038 7.1:+677683 MS.gene025890:CDS
CGAGGCTTCTTCTGTGCTGT+TGG 0.425164 7.1:-677673 None:intergenic
TACCATGGTATTGACACCTT+TGG 0.431203 7.1:-676338 None:intergenic
ATCTGAAAGGAATTAGTTCT+GGG 0.433081 7.1:+678446 MS.gene025890:CDS
ACTAGCAATGTGCCATTTCA+AGG 0.436943 7.1:+678838 MS.gene025890:CDS
GCAAGAACACGCATTCCAAT+AGG 0.445918 7.1:-676270 None:intergenic
TGAGAGAACTGAATCTTGAA+TGG 0.446366 7.1:-678492 None:intergenic
TGTACCTCTTTCAATGTGAC+AGG 0.446657 7.1:-677451 None:intergenic
AATGGTCTAAAAGCACTGTT+AGG 0.452029 7.1:-678568 None:intergenic
TGAGCTTGTTGGACTTGAGA+TGG 0.457732 7.1:+677256 MS.gene025890:CDS
AAACTTGCGGTGTAAGTCAT+TGG 0.458916 7.1:-677707 None:intergenic
TTCAGTTCTCTCACTAGAGT+TGG 0.460910 7.1:+678502 MS.gene025890:CDS
TCTCGTTCAACATTTACAAC+AGG 0.467597 7.1:-678919 None:intergenic
AGCTTAAATCCATCCATGTC+TGG 0.471845 7.1:-677230 None:intergenic
CAATATGCATGGATTTCAAC+CGG 0.482097 7.1:+678639 MS.gene025890:CDS
AGACCATTTCCATCTGGTGG+TGG 0.485592 7.1:+678583 MS.gene025890:CDS
GGATAAGAATGATGATATTG+AGG 0.489929 7.1:+677618 MS.gene025890:CDS
CCATGCATATTGACACTAGC+AGG 0.493898 7.1:-678628 None:intergenic
ATACCACCACCAGATGGAAA+TGG 0.500539 7.1:-678586 None:intergenic
TGACTTTGATGACGTCTTCG+AGG 0.509927 7.1:-679029 None:intergenic
TAATGTGGCCAGCCATCTCC+AGG 0.513129 7.1:+678139 MS.gene025890:CDS
TTTAGACCATTTCCATCTGG+TGG 0.515382 7.1:+678580 MS.gene025890:CDS
TCTTTGAACATGGTTCGGAT+TGG 0.518790 7.1:-678674 None:intergenic
CATCAAAGTCATGACCAAAC+AGG 0.521398 7.1:+679041 MS.gene025890:CDS
GGATTGGCGTGAGCTTTGGC+CGG 0.526522 7.1:-678658 None:intergenic
CAATTATATGCTAATTACTC+TGG 0.530133 7.1:+679886 MS.gene025890:CDS
GACTTTGATGACGTCTTCGA+GGG 0.537259 7.1:-679028 None:intergenic
TATGAACATTACTGATTACA+AGG 0.547400 7.1:-677205 None:intergenic
TTGTAAATGTTGAACGAGAA+GGG 0.549447 7.1:+678923 MS.gene025890:CDS
GAAACCTGTCACATTGAAAG+AGG 0.553386 7.1:+677447 MS.gene025890:CDS
CCTGCTAGTGTCAATATGCA+TGG 0.555451 7.1:+678628 MS.gene025890:CDS
CTCATCATATATGCCTCATG+TGG 0.556475 7.1:-678958 None:intergenic
TGAATGTTGAAAATCTTACA+AGG 0.561053 7.1:+678114 MS.gene025890:CDS
GTTGTAAATGTTGAACGAGA+AGG 0.564050 7.1:+678922 MS.gene025890:CDS
GAGAGAACTGAATCTTGAAT+GGG 0.567242 7.1:-678491 None:intergenic
GCAATGTGCCATTTCAAGGA+TGG 0.575878 7.1:+678842 MS.gene025890:CDS
TATATGCTAATTACTCTGGT+GGG 0.582478 7.1:+679890 MS.gene025890:CDS
TTATATGCTAATTACTCTGG+TGG 0.583480 7.1:+679889 MS.gene025890:CDS
GTTCATATGCCAGACATGGA+TGG 0.584384 7.1:+677221 MS.gene025890:CDS
AAATCTTACAAGGGATAATG+TGG 0.585895 7.1:+678124 MS.gene025890:CDS
AAATTGGCATTCTTTGAACA+TGG 0.589855 7.1:-678684 None:intergenic
GGTTCGTGCACTTTGAGCAG+TGG 0.595278 7.1:-677144 None:intergenic
AAATATAGACTCTATCTGAA+AGG 0.596380 7.1:+678433 MS.gene025890:CDS
TTGCATAACTCACTTATGAC+AGG 0.597051 7.1:-677284 None:intergenic
AAATTCTTACCTTCCACAAG+GGG 0.600441 7.1:+678801 MS.gene025890:CDS
CTACTCATACCACCACCAGA+TGG 0.600621 7.1:-678592 None:intergenic
TAAGAATGATGATATTGAGG+AGG 0.601304 7.1:+677621 MS.gene025890:CDS
GTGATGAAAGGAATTGATCA+TGG 0.607173 7.1:+677406 MS.gene025890:CDS
TGATGATAGAACTTGTCTCA+AGG 0.612252 7.1:+676299 MS.gene025890:CDS
CAAATTCTTACCTTCCACAA+GGG 0.612572 7.1:+678800 MS.gene025890:CDS
TAATGTTCATATGCCAGACA+TGG 0.614385 7.1:+677217 MS.gene025890:CDS
AGAAAACAGTGGATGTTGAG+AGG 0.619813 7.1:-678716 None:intergenic
TTTCTGCAAATGATGATCCA+AGG 0.640841 7.1:+677380 MS.gene025890:CDS
GATCCAAGGATGGTGATGAA+AGG 0.644591 7.1:+677394 MS.gene025890:CDS
ACAAATTCTTACCTTCCACA+AGG 0.649192 7.1:+678799 MS.gene025890:CDS
CTCCAAAGGTGTCAATACCA+TGG 0.650196 7.1:+676336 MS.gene025890:CDS
ATATATGATGAGCAACAAGA+TGG 0.653513 7.1:+678969 MS.gene025890:CDS
TGCAAATGATGATCCAAGGA+TGG 0.655257 7.1:+677384 MS.gene025890:CDS
GAATGTTGAAAATCTTACAA+GGG 0.660347 7.1:+678115 MS.gene025890:CDS
ATAACTCACTTATGACAGGT+AGG 0.663328 7.1:-677280 None:intergenic
TGTAAAGAGCAAGAAAACAG+TGG 0.667428 7.1:-678727 None:intergenic
TCATTGGACCAAACAACTCG+AGG 0.676972 7.1:-677691 None:intergenic
TTCTCTCAACAAGTTCAATG+CGG 0.677431 7.1:-677165 None:intergenic
GCCTTCAAAGAAACCACATG+AGG 0.683194 7.1:+678945 MS.gene025890:CDS
TCTGAAAGGAATTAGTTCTG+GGG 0.683638 7.1:+678447 MS.gene025890:CDS
TAACAACAGCAAGAAACTTG+CGG 0.687001 7.1:-677720 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATAAATCTAATCGT+TGG - chr7.1:677019-677038 None:intergenic 15.0%
!!! AAACTTTAAAGATATATACA+TGG - chr7.1:679811-679830 None:intergenic 15.0%
!!! AATTTTCTTTGATATTGTTT+AGG + chr7.1:676783-676802 MS.gene025890:intron 15.0%
!!! ATATCTATTTTTTCTACATT+AGG + chr7.1:676720-676739 MS.gene025890:intron 15.0%
!!! ATATGATTATATCATTCTTT+TGG - chr7.1:679611-679630 None:intergenic 15.0%
!! AATAATTAATTCGCTAAACT+TGG + chr7.1:679681-679700 MS.gene025890:intron 20.0%
!! AGAATGATATAATCATATAG+AGG + chr7.1:679613-679632 MS.gene025890:intron 20.0%
!! ATAAAGACGAATATATCAAT+TGG - chr7.1:677817-677836 None:intergenic 20.0%
!! ATCGTCAAAAAATCATATAA+TGG + chr7.1:679148-679167 MS.gene025890:intron 20.0%
!! ATGTAATATAATCTAACGTA+TGG + chr7.1:679101-679120 MS.gene025890:intron 20.0%
!! CATAAATTATATTACCTGTT+TGG - chr7.1:679058-679077 None:intergenic 20.0%
!! TATCAAAGAAAATTTAAGTC+TGG - chr7.1:676777-676796 None:intergenic 20.0%
!! TATTAGATACAACATAGATT+CGG + chr7.1:676413-676432 MS.gene025890:intron 20.0%
!! TTTAATTGCATAGAAATTGT+TGG + chr7.1:676959-676978 MS.gene025890:intron 20.0%
!!! ATCAATTCAAGAATCTTTTT+TGG - chr7.1:678095-678114 None:intergenic 20.0%
!!! ATTTCATAGATTTTATGTTG+TGG + chr7.1:677048-677067 MS.gene025890:intron 20.0%
!!! TGCTGCTTAAATATTAAAAA+TGG + chr7.1:676364-676383 MS.gene025890:intron 20.0%
!!! TTTTGTTCTTTAAATCCTTT+TGG + chr7.1:676514-676533 MS.gene025890:intron 20.0%
! AAAAAGGTTCAATGACATTT+GGG + chr7.1:678753-678772 MS.gene025890:CDS 25.0%
! AAATATAGACTCTATCTGAA+AGG + chr7.1:678433-678452 MS.gene025890:CDS 25.0%
! AAATGTATCAGTAACAAGAT+TGG - chr7.1:677314-677333 None:intergenic 25.0%
! AACCATAATAATCATGTGAT+TGG + chr7.1:678871-678890 MS.gene025890:CDS 25.0%
! AATATATACTTTACCTCGTT+TGG + chr7.1:679773-679792 MS.gene025890:intron 25.0%
! AATCTGTGAAAGAATTTGAT+CGG - chr7.1:676829-676848 None:intergenic 25.0%
! CAATTATATGCTAATTACTC+TGG + chr7.1:679886-679905 MS.gene025890:CDS 25.0%
! GAATGTTGAAAATCTTACAA+GGG + chr7.1:678115-678134 MS.gene025890:CDS 25.0%
! GTCAAAATCAATTATTGTCA+CGG - chr7.1:679188-679207 None:intergenic 25.0%
! TACCAATCACATGATTATTA+TGG - chr7.1:678876-678895 None:intergenic 25.0%
! TCAAGTAGAATCAATTATGA+AGG + chr7.1:679320-679339 MS.gene025890:intron 25.0%
! TGAATGTTGAAAATCTTACA+AGG + chr7.1:678114-678133 MS.gene025890:CDS 25.0%
! TTAATTCACGAAAACTTACT+TGG + chr7.1:678376-678395 MS.gene025890:intron 25.0%
! TTGAATATGTTTGTTTGAGT+AGG + chr7.1:677851-677870 MS.gene025890:intron 25.0%
!! AATTGATTCATACAAAGCAT+TGG - chr7.1:676751-676770 None:intergenic 25.0%
!! TATGAACATTACTGATTACA+AGG - chr7.1:677208-677227 None:intergenic 25.0%
!! TCTTTGATATTGTTTAGGTT+CGG + chr7.1:676788-676807 MS.gene025890:intron 25.0%
!! TTTTTATTCCATCCTTGAAA+TGG - chr7.1:678853-678872 None:intergenic 25.0%
!!! GTCAGTTTGTTTGTTTATTT+TGG + chr7.1:676622-676641 MS.gene025890:intron 25.0%
!!! TGTGATCTTATGTTGTTTTT+TGG + chr7.1:676926-676945 MS.gene025890:intron 25.0%
AAATCTTACAAGGGATAATG+TGG + chr7.1:678124-678143 MS.gene025890:CDS 30.0%
AAATTGGCATTCTTTGAACA+TGG - chr7.1:678687-678706 None:intergenic 30.0%
AAGAGATAAAGAAAAACACC+TGG - chr7.1:678160-678179 None:intergenic 30.0%
AATCATATGTGTTTGTGTGT+AGG + chr7.1:676473-676492 MS.gene025890:intron 30.0%
ACACATATCTCCAAAACTTA+AGG - chr7.1:676692-676711 None:intergenic 30.0%
ACGAATAAACCACATACTTA+CGG - chr7.1:679585-679604 None:intergenic 30.0%
ATATATGATGAGCAACAAGA+TGG + chr7.1:678969-678988 MS.gene025890:CDS 30.0%
ATATCAATTGGATTGCTATG+AGG - chr7.1:677805-677824 None:intergenic 30.0%
ATGAAAAGCCAACTAGAAAA+AGG + chr7.1:677593-677612 MS.gene025890:CDS 30.0%
ATGAATGATCAGTTTCCTAT+TGG + chr7.1:676255-676274 MS.gene025890:CDS 30.0%
CATATAGAGGTAGCAATAAA+CGG + chr7.1:679626-679645 MS.gene025890:intron 30.0%
CCATTTCCACTATCAAAATT+AGG - chr7.1:677538-677557 None:intergenic 30.0%
GAAAAAGGTTCAATGACATT+TGG + chr7.1:678752-678771 MS.gene025890:CDS 30.0%
GATCAAATTCTTTCACAGAT+TGG + chr7.1:676828-676847 MS.gene025890:intron 30.0%
GGATAAGAATGATGATATTG+AGG + chr7.1:677618-677637 MS.gene025890:CDS 30.0%
TAAGAATGATGATATTGAGG+AGG + chr7.1:677621-677640 MS.gene025890:CDS 30.0%
TACTCTCCTATGATTAATCT+AGG + chr7.1:677955-677974 MS.gene025890:intron 30.0%
TAGTTGTACCTATCCTTTAA+GGG - chr7.1:679439-679458 None:intergenic 30.0%
TATATGCTAATTACTCTGGT+GGG + chr7.1:679890-679909 MS.gene025890:CDS 30.0%
TATCGAAAAACTTCATAGCA+CGG - chr7.1:678023-678042 None:intergenic 30.0%
TATCTGAAAGGAATTAGTTC+TGG + chr7.1:678445-678464 MS.gene025890:CDS 30.0%
TTATATGCTAATTACTCTGG+TGG + chr7.1:679889-679908 MS.gene025890:CDS 30.0%
TTCACTCCTAGATTAATCAT+AGG - chr7.1:677964-677983 None:intergenic 30.0%
TTGTAAATGTTGAACGAGAA+GGG + chr7.1:678923-678942 MS.gene025890:CDS 30.0%
TTTGTGTGAAAATCAATTCC+AGG + chr7.1:679274-679293 MS.gene025890:intron 30.0%
! ATCTGAAAGGAATTAGTTCT+GGG + chr7.1:678446-678465 MS.gene025890:CDS 30.0%
! ATTGATTCTACTTGAAGCTA+TGG - chr7.1:679315-679334 None:intergenic 30.0%
! TGATTTGGCAACATGTAATT+AGG + chr7.1:677476-677495 MS.gene025890:CDS 30.0%
! TTTGACAATAAGTGAGTTGA+AGG + chr7.1:679202-679221 MS.gene025890:intron 30.0%
!! AAGGTAAAGTGTTTTGTGTT+TGG + chr7.1:679221-679240 MS.gene025890:intron 30.0%
!! CTTGCTGTTGTTAATCAATT+AGG + chr7.1:677730-677749 MS.gene025890:CDS 30.0%
!! TAATCATAGGAGAGTATTTG+AGG - chr7.1:677951-677970 None:intergenic 30.0%
!! TTATCCTTCCTTTTTCTAGT+TGG - chr7.1:677604-677623 None:intergenic 30.0%
!!! CCTAATTTTGATAGTGGAAA+TGG + chr7.1:677535-677554 MS.gene025890:CDS 30.0%
!!! TGAATTTAGTTTTCAGCTCA+AGG - chr7.1:679728-679747 None:intergenic 30.0%
!!! TTGTGACACAACTTTTTTGA+CGG + chr7.1:676651-676670 MS.gene025890:intron 30.0%
AAAAGAGTTGCATTACATCC+TGG - chr7.1:676996-677015 None:intergenic 35.0%
AAAGCCAACTAGAAAAAGGA+AGG + chr7.1:677597-677616 MS.gene025890:CDS 35.0%
AAAGGATAGGTACAACTACA+GGG + chr7.1:679441-679460 MS.gene025890:intron 35.0%
AAATTCTTACCTTCCACAAG+GGG + chr7.1:678801-678820 MS.gene025890:CDS 35.0%
AATGGGATGTATTTGCTTGT+TGG - chr7.1:678477-678496 None:intergenic 35.0%
ACAAATTCTTACCTTCCACA+AGG + chr7.1:678799-678818 MS.gene025890:CDS 35.0%
ATAACTCACTTATGACAGGT+AGG - chr7.1:677283-677302 None:intergenic 35.0%
CAAATTCTTACCTTCCACAA+GGG + chr7.1:678800-678819 MS.gene025890:CDS 35.0%
CAATATGCATGGATTTCAAC+CGG + chr7.1:678639-678658 MS.gene025890:CDS 35.0%
CTGTTAAAAATAACCCCTTG+TGG - chr7.1:678817-678836 None:intergenic 35.0%
CTTGCTCTTTACATGGAAAA+AGG + chr7.1:678737-678756 MS.gene025890:CDS 35.0%
GAGAGAACTGAATCTTGAAT+GGG - chr7.1:678494-678513 None:intergenic 35.0%
GTAAGTATGTGGTTTATTCG+TGG + chr7.1:679584-679603 MS.gene025890:intron 35.0%
GTAGTTGTACCTATCCTTTA+AGG - chr7.1:679440-679459 None:intergenic 35.0%
GTGATGAAAGGAATTGATCA+TGG + chr7.1:677406-677425 MS.gene025890:CDS 35.0%
GTTCCAACGTATATTTGAGT+TGG - chr7.1:679654-679673 None:intergenic 35.0%
GTTGTAAATGTTGAACGAGA+AGG + chr7.1:678922-678941 MS.gene025890:CDS 35.0%
TAAAAATAACCCCTTGTGGA+AGG - chr7.1:678813-678832 None:intergenic 35.0%
TAAAGAAAAACACCTGGAGA+TGG - chr7.1:678154-678173 None:intergenic 35.0%
TAAAGGATAGGTACAACTAC+AGG + chr7.1:679440-679459 MS.gene025890:intron 35.0%
TAACAACAGCAAGAAACTTG+CGG - chr7.1:677723-677742 None:intergenic 35.0%
TAATGTTCATATGCCAGACA+TGG + chr7.1:677217-677236 MS.gene025890:CDS 35.0%
TACAACATAGATTCGGACTT+CGG + chr7.1:676420-676439 MS.gene025890:intron 35.0%
TCATCATGATTACAATGCCT+AGG - chr7.1:676540-676559 None:intergenic 35.0%
TCTCGTTCAACATTTACAAC+AGG - chr7.1:678922-678941 None:intergenic 35.0%
TGAGAGAACTGAATCTTGAA+TGG - chr7.1:678495-678514 None:intergenic 35.0%
TGATGATAGAACTTGTCTCA+AGG + chr7.1:676299-676318 MS.gene025890:CDS 35.0%
TGTAAAGAGCAAGAAAACAG+TGG - chr7.1:678730-678749 None:intergenic 35.0%
TTCACAGATTGGACATGATT+TGG + chr7.1:676839-676858 MS.gene025890:intron 35.0%
TTCTCTCAACAAGTTCAATG+CGG - chr7.1:677168-677187 None:intergenic 35.0%
TTGCATAACTCACTTATGAC+AGG - chr7.1:677287-677306 None:intergenic 35.0%
TTGTTGGATTCATTAGATCC+AGG + chr7.1:676975-676994 MS.gene025890:intron 35.0%
TTTAAGCTTCTTGAGCTTGT+TGG + chr7.1:677245-677264 MS.gene025890:CDS 35.0%
TTTCTGCAAATGATGATCCA+AGG + chr7.1:677380-677399 MS.gene025890:CDS 35.0%
! AAAGATAACACCTTCTAGAG+AGG - chr7.1:679400-679419 None:intergenic 35.0%
! AAGATAACACCTTCTAGAGA+GGG - chr7.1:679399-679418 None:intergenic 35.0%
! CATGTGATTGGTAGTTCAAT+AGG + chr7.1:678883-678902 MS.gene025890:CDS 35.0%
! CTGTTTTCTTGCTCTTTACA+TGG + chr7.1:678730-678749 MS.gene025890:CDS 35.0%
! GATGGTGTTTCCTTAAGTTT+TGG + chr7.1:676679-676698 MS.gene025890:intron 35.0%
! GGAAGGTAAGAATTTGTTTC+AGG - chr7.1:678796-678815 None:intergenic 35.0%
! TAGAACTTGTCTCAAGGTTT+TGG + chr7.1:676305-676324 MS.gene025890:CDS 35.0%
! TCTTTAAATCCTTTTGGCCT+AGG + chr7.1:676520-676539 MS.gene025890:intron 35.0%
! TGAAAGAGGTACAGATGATT+TGG + chr7.1:677461-677480 MS.gene025890:CDS 35.0%
!! AATGGTCTAAAAGCACTGTT+AGG - chr7.1:678571-678590 None:intergenic 35.0%
!! GATGTGCCTAATTTTGATAG+TGG + chr7.1:677529-677548 MS.gene025890:CDS 35.0%
!! TCTGAAAGGAATTAGTTCTG+GGG + chr7.1:678447-678466 MS.gene025890:CDS 35.0%
!!! ACTTTTTTGACGGATCTTGA+TGG + chr7.1:676661-676680 MS.gene025890:intron 35.0%
AAACTTGCGGTGTAAGTCAT+TGG - chr7.1:677710-677729 None:intergenic 40.0%
AGAAAACAGTGGATGTTGAG+AGG - chr7.1:678719-678738 None:intergenic 40.0%
AGCTTAAATCCATCCATGTC+TGG - chr7.1:677233-677252 None:intergenic 40.0%
ATTACAATGCCTAGGCCAAA+AGG - chr7.1:676532-676551 None:intergenic 40.0%
ATTCCTTTCATCACCATCCT+TGG - chr7.1:677400-677419 None:intergenic 40.0%
ATTTAAGCAGCAGCATACCA+TGG - chr7.1:676356-676375 None:intergenic 40.0%
CAATTTGTACCCTCTCTAGA+AGG + chr7.1:679387-679406 MS.gene025890:intron 40.0%
CAGCTCAAGGTCAATTCATT+TGG - chr7.1:679715-679734 None:intergenic 40.0%
CATCAAAGTCATGACCAAAC+AGG + chr7.1:679041-679060 MS.gene025890:CDS 40.0%
CATGATCGCAAAACCCTTAA+AGG + chr7.1:679423-679442 MS.gene025890:intron 40.0%
CGATTAGACTCAAGTCCAAA+AGG - chr7.1:679495-679514 None:intergenic 40.0%
CTCATCATATATGCCTCATG+TGG - chr7.1:678961-678980 None:intergenic 40.0%
GAAACCTGTCACATTGAAAG+AGG + chr7.1:677447-677466 MS.gene025890:CDS 40.0%
GAGCCAACTCAAATATACGT+TGG + chr7.1:679648-679667 MS.gene025890:intron 40.0%
GGCATTCTTTGAACATGGTT+CGG - chr7.1:678682-678701 None:intergenic 40.0%
GTTTAATCGTGTACCAAACG+AGG - chr7.1:679789-679808 None:intergenic 40.0%
TCGCAAAACCCTTAAAGGAT+AGG + chr7.1:679428-679447 MS.gene025890:intron 40.0%
TGAGAGGCTACAGAAGAAAT+TGG - chr7.1:678703-678722 None:intergenic 40.0%
TGCAAATGATGATCCAAGGA+TGG + chr7.1:677384-677403 MS.gene025890:CDS 40.0%
TGTACCTCTTTCAATGTGAC+AGG - chr7.1:677454-677473 None:intergenic 40.0%
TTCAGTTCTCTCACTAGAGT+TGG + chr7.1:678502-678521 MS.gene025890:CDS 40.0%
TTTAGACCATTTCCATCTGG+TGG + chr7.1:678580-678599 MS.gene025890:CDS 40.0%
! GCTTTTAGACCATTTCCATC+TGG + chr7.1:678577-678596 MS.gene025890:CDS 40.0%
! TCTTTGAACATGGTTCGGAT+TGG - chr7.1:678677-678696 None:intergenic 40.0%
!! AAGGGTGTGTGTAATCCTTT+TGG + chr7.1:679477-679496 MS.gene025890:intron 40.0%
!! ACTAGCAATGTGCCATTTCA+AGG + chr7.1:678838-678857 MS.gene025890:CDS 40.0%
!! GATTCATACAAAGCATTGGC+TGG - chr7.1:676747-676766 None:intergenic 40.0%
!! GTTGATAGAAGGTGTTGAGA+AGG + chr7.1:679522-679541 MS.gene025890:intron 40.0%
!! TACCATGGTATTGACACCTT+TGG - chr7.1:676341-676360 None:intergenic 40.0%
!! TTTTGGAGAGACTTCTCCAA+AGG + chr7.1:676322-676341 MS.gene025890:CDS 40.0%
ATACCACCACCAGATGGAAA+TGG - chr7.1:678589-678608 None:intergenic 45.0%
CCATGCATATTGACACTAGC+AGG - chr7.1:678631-678650 None:intergenic 45.0%
CCTGCTAGTGTCAATATGCA+TGG + chr7.1:678628-678647 MS.gene025890:CDS 45.0%
GCAAGAACACGCATTCCAAT+AGG - chr7.1:676273-676292 None:intergenic 45.0%
GCACATGCACTTCAGATTGA+AGG - chr7.1:678337-678356 None:intergenic 45.0%
GCCTCATGTGGTTTCTTTGA+AGG - chr7.1:678949-678968 None:intergenic 45.0%
GCCTTCAAAGAAACCACATG+AGG + chr7.1:678945-678964 MS.gene025890:CDS 45.0%
GTTCATATGCCAGACATGGA+TGG + chr7.1:677221-677240 MS.gene025890:CDS 45.0%
TCATTGGACCAAACAACTCG+AGG - chr7.1:677694-677713 None:intergenic 45.0%
! AGAAGAAGCCTCGAGTTGTT+TGG + chr7.1:677683-677702 MS.gene025890:CDS 45.0%
! GATCCAAGGATGGTGATGAA+AGG + chr7.1:677394-677413 MS.gene025890:CDS 45.0%
! TGGTGTGTAGCTTTTGATCC+TGG - chr7.1:679295-679314 None:intergenic 45.0%
!! ATCGTCTGCGAGTTGATAGA+AGG + chr7.1:679511-679530 MS.gene025890:intron 45.0%
!! CTCCAAAGGTGTCAATACCA+TGG + chr7.1:676336-676355 MS.gene025890:CDS 45.0%
!! GACTTTGATGACGTCTTCGA+GGG - chr7.1:679031-679050 None:intergenic 45.0%
!! GATAGAAGGTGTTGAGAAGG+AGG + chr7.1:679525-679544 MS.gene025890:intron 45.0%
!! GCAATGTGCCATTTCAAGGA+TGG + chr7.1:678842-678861 MS.gene025890:CDS 45.0%
!! TGACTTTGATGACGTCTTCG+AGG - chr7.1:679032-679051 None:intergenic 45.0%
!! TGAGCTTGTTGGACTTGAGA+TGG + chr7.1:677256-677275 MS.gene025890:CDS 45.0%
ACAGGGTGCATATCTTCGCA+AGG + chr7.1:679458-679477 MS.gene025890:intron 50.0%
AGACCATTTCCATCTGGTGG+TGG + chr7.1:678583-678602 MS.gene025890:CDS 50.0%
ATACTTACGGCTGACGAAGG+CGG - chr7.1:679572-679591 None:intergenic 50.0%
CACATACTTACGGCTGACGA+AGG - chr7.1:679575-679594 None:intergenic 50.0%
CAGGGTGCATATCTTCGCAA+GGG + chr7.1:679459-679478 MS.gene025890:intron 50.0%
CTACTCATACCACCACCAGA+TGG - chr7.1:678595-678614 None:intergenic 50.0%
CTTCGTCAGCCGTAAGTATG+TGG + chr7.1:679573-679592 MS.gene025890:intron 50.0%
GAAAAACACCTGGAGATGGC+TGG - chr7.1:678150-678169 None:intergenic 50.0%
CGAGGCTTCTTCTGTGCTGT+TGG - chr7.1:677676-677695 None:intergenic 55.0%
GGTTCGTGCACTTTGAGCAG+TGG - chr7.1:677147-677166 None:intergenic 55.0%
TAATGTGGCCAGCCATCTCC+AGG + chr7.1:678139-678158 MS.gene025890:CDS 55.0%
!! GTTCGGATTGGCGTGAGCTT+TGG - chr7.1:678665-678684 None:intergenic 55.0%
AGGAGGCCTCCGTCGTTGAT+CGG + chr7.1:679542-679561 MS.gene025890:intron 60.0%
! GGATTGGCGTGAGCTTTGGC+CGG - chr7.1:678661-678680 None:intergenic 60.0%
GGATCGCCGATCAACGACGG+AGG - chr7.1:679551-679570 None:intergenic 65.0%
GGCGGATCGCCGATCAACGA+CGG - chr7.1:679554-679573 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 676255 679924 676255 ID=MS.gene025890
chr7.1 mRNA 676255 679924 676255 ID=MS.gene025890.t1;Parent=MS.gene025890
chr7.1 exon 676255 676357 676255 ID=MS.gene025890.t1.exon1;Parent=MS.gene025890.t1
chr7.1 CDS 676255 676357 676255 ID=cds.MS.gene025890.t1;Parent=MS.gene025890.t1
chr7.1 exon 677141 677293 677141 ID=MS.gene025890.t1.exon2;Parent=MS.gene025890.t1
chr7.1 CDS 677141 677293 677141 ID=cds.MS.gene025890.t1;Parent=MS.gene025890.t1
chr7.1 exon 677377 677760 677377 ID=MS.gene025890.t1.exon3;Parent=MS.gene025890.t1
chr7.1 CDS 677377 677760 677377 ID=cds.MS.gene025890.t1;Parent=MS.gene025890.t1
chr7.1 exon 678084 678160 678084 ID=MS.gene025890.t1.exon4;Parent=MS.gene025890.t1
chr7.1 CDS 678084 678160 678084 ID=cds.MS.gene025890.t1;Parent=MS.gene025890.t1
chr7.1 exon 678433 679062 678433 ID=MS.gene025890.t1.exon5;Parent=MS.gene025890.t1
chr7.1 CDS 678433 679062 678433 ID=cds.MS.gene025890.t1;Parent=MS.gene025890.t1
chr7.1 exon 679883 679924 679883 ID=MS.gene025890.t1.exon6;Parent=MS.gene025890.t1
chr7.1 CDS 679883 679924 679883 ID=cds.MS.gene025890.t1;Parent=MS.gene025890.t1
Gene Sequence

>MS.gene025890

ATGAATGATCAGTTTCCTATTGGAATGCGTGTTCTTGCTGTTGATGATGATAGAACTTGTCTCAAGGTTTTGGAGAGACTTCTCCAAAGGTGTCAATACCATGGTATGCTGCTGCTTAAATATTAAAAATGGATTGTTGACTAAGTAGTGACACAGAATATTAGATACAACATAGATTCGGACTTCGGTTTTAATTTTATAAAATGACATTTGAATGTAATCATATGTGTTTGTGTGTAGGTGCTACTGAAGTTTTTGATTTTGTTCTTTAAATCCTTTTGGCCTAGGCATTGTAATCATGATGATAACATTAATAGTTTCTGTTTTTTTTTTTTTGTTTGTTGTAAACTTGAATTTGTCAAGTTGAGTCAGTTTGTTTGTTTATTTTGGTTTTTGTTGTGACACAACTTTTTTGACGGATCTTGATGGTGTTTCCTTAAGTTTTGGAGATATGTGTATTTATGTATATCTATTTTTTCTACATTAGGTCCAGCCAATGCTTTGTATGAATCAATTATGCCAGACTTAAATTTTCTTTGATATTGTTTAGGTTCGGCTAAATTATATTTGACCGATCAAATTCTTTCACAGATTGGACATGATTTGGTTTTTAAAGTTCAAATTTTTAAAAAAATAAATAAAAATAAATAAAAATTGTGTGTCTAATCTTTTGTGATCTTATGTTGTTTTTTGGATTTCAAAGATTTAATTGCATAGAAATTGTTGGATTCATTAGATCCAGGATGTAATGCAACTCTTTTCCAACGATTAGATTTATTATTTTTAAAGTAACATTTCATAGATTTTATGTTGTGGTTGTATCTGCAATGTATCATTTTTACTCTTTCAAAATGATATTAATTGTAGTTATGATTATCTGTTTCAGTAACCACTGCTCAAAGTGCACGAACCGCATTGAACTTGTTGAGAGAAAACAAAAGCAATTTTGACCTTGTAATCAGTAATGTTCATATGCCAGACATGGATGGATTTAAGCTTCTTGAGCTTGTTGGACTTGAGATGGACCTACCTGTCATAAGTGAGTTATGCAATTCTCCAATCTTGTTACTGATACATTTATAACAAATTATAGTGTTTTGATTACAAAGTTTTTGATTTCAGTGTTTTCTGCAAATGATGATCCAAGGATGGTGATGAAAGGAATTGATCATGGTGCTTGTGATTATCTGCTGAAACCTGTCACATTGAAAGAGGTACAGATGATTTGGCAACATGTAATTAGGAAGAAGAAAACTAGCAAAAGAAGCAATCATGATGTGCCTAATTTTGATAGTGGAAATGGAATAGATTCAGCTGTGACAAGAAATTCTGATCAAAATGAAAAGCCAACTAGAAAAAGGAAGGATAAGAATGATGATATTGAGGAGGAGAATGAAGATGATCATGACAATGATGATCCAACAGCACAGAAGAAGCCTCGAGTTGTTTGGTCCAATGACTTACACCGCAAGTTTCTTGCTGTTGTTAATCAATTAGGCATTGAAAGTACGTTCTTTCGTCTGTCTTCAATGCGCAAAAATAATTGTCCTCATAGCAATCCAATTGATATATTCGTCTTTATTTTTTCTCATTTTATTGAATATGTTTGTTTGAGTAGGTTATAGCAATTCAATGTTGAGATTAATATTATAATATGAAAATGAAAATTTTACTTTGTATTATTTGAAAACTTCCTCAAATACTCTCCTATGATTAATCTAGGAGTGAATGTGAATCTCATGTCATGATCATTACTCATGTTTAACCGTGCTATGAAGTTTTTCGATACTAAACTGTGTTTCTTATTTTAGTTGTTATTTTGCTTGCAGATGCTATACCAAAAAAGATTCTTGAATTGATGAATGTTGAAAATCTTACAAGGGATAATGTGGCCAGCCATCTCCAGGTGTTTTTCTTTATCTCTTATGCTTTACTTTTATACGCTATGAAGACAACAATGACATTAACGTAGATGCTAAACATGACACTGACATAGTGACACTGCTAATAATTTACAAATTTGAAGTAATTAAATTAAATGTAATCACACGTATCAGCGTCTGACACTAGACAAACACACCTTCAATCTGAAGTGCATGTGCTACGAAGTTCTCAAGCACTTTAATTCACGAAAACTTACTTGGTTCTTTTTTTTTTTTTTTTTTTTTAAATCTGCAGAAATATAGACTCTATCTGAAAGGAATTAGTTCTGGGGAAAACCAACAAGCAAATACATCCCATTCAAGATTCAGTTCTCTCACTAGAGTTGGAGAACATTTTCATACATTGAATAACTCTAGACAGTTTCATAATCCTAACAGTGCTTTTAGACCATTTCCATCTGGTGGTGGTATGAGTAGCAGATTGAACACTCCTGCTAGTGTCAATATGCATGGATTTCAACCGGCCAAAGCTCACGCCAATCCGAACCATGTTCAAAGAATGCCAATTTCTTCTGTAGCCTCTCAACATCCACTGTTTTCTTGCTCTTTACATGGAAAAAGGTTCAATGACATTTGGGCAAGCACTACGCAATTACCTGAAACAAATTCTTACCTTCCACAAGGGGTTATTTTTAACAGCACTAGCAATGTGCCATTTCAAGGATGGAATAAAAACCATAATAATCATGTGATTGGTAGTTCAATAGGCTCTTCTACAACTCCTGTTGTAAATGTTGAACGAGAAGGGCCTTCAAAGAAACCACATGAGGCATATATGATGAGCAACAAGATGGAGTCTAAAAGTTGCTGTATCTCTAATGATATTTGTTCCCTCGAAGACGTCATCAAAGTCATGACCAAACAGGTAATATAATTTATGCAATGTGATGAAGTTCTTATGCAATGTAATATAATCTAACGTATGGTTGTTGCTTTGAGCATAATTAATTATCGTCAAAAAATCATATAATGGCTTAAACATGTCTGCCGTGACAATAATTGATTTTGACAATAAGTGAGTTGAAGGTAAAGTGTTTTGTGTTTGGAACATATGTAAAAATGAGTTGAACTATAAATTTGTGTGAAAATCAATTCCAGGATCAAAAGCTACACACCATAGCTTCAAGTAGAATCAATTATGAAGGTAGAATCAATTCTTATTCTACTTAAAAAAAAATAATGTCAAAATCAATTTGTACCCTCTCTAGAAGGTGTTATCTTTGCGCATGATCGCAAAACCCTTAAAGGATAGGTACAACTACAGGGTGCATATCTTCGCAAGGGTGTGTGTAATCCTTTTGGACTTGAGTCTAATCGTCTGCGAGTTGATAGAAGGTGTTGAGAAGGAGGCCTCCGTCGTTGATCGGCGATCCGCCTTCGTCAGCCGTAAGTATGTGGTTTATTCGTGGACCAAAAGAATGATATAATCATATAGAGGTAGCAATAAACGGAGCCAACTCAAATATACGTTGGAACTCAATTTAATAATTAATTCGCTAAACTTGGTTTATGAACCAAATGAATTGACCTTGAGCTGAAAACTAAATTCATTAACTAAATAAGCAGAACTTGAGCAATATATACTTTACCTCGTTTGGTACACGATTAAACCATGTATATATCTTTAAAGTTTTATTATACAATTTTCTCTATTGCTTGTTGATAAATATTTTCTTTTGATGCAGAAACAATTATATGCTAATTACTCTGGTGGGAAATCAAAGTGA
Protein sequence

>MS.gene025890.t1

MNDQFPIGMRVLAVDDDRTCLKVLERLLQRCQYHVTTAQSARTALNLLRENKSNFDLVISNVHMPDMDGFKLLELVGLEMDLPVIMFSANDDPRMVMKGIDHGACDYLLKPVTLKEVQMIWQHVIRKKKTSKRSNHDVPNFDSGNGIDSAVTRNSDQNEKPTRKRKDKNDDIEEENEDDHDNDDPTAQKKPRVVWSNDLHRKFLAVVNQLGIENAIPKKILELMNVENLTRDNVASHLQKYRLYLKGISSGENQQANTSHSRFSSLTRVGEHFHTLNNSRQFHNPNSAFRPFPSGGGMSSRLNTPASVNMHGFQPAKAHANPNHVQRMPISSVASQHPLFSCSLHGKRFNDIWASTTQLPETNSYLPQGVIFNSTSNVPFQGWNKNHNNHVIGSSIGSSTTPVVNVEREGPSKKPHEAYMMSNKMESKSCCISNDICSLEDVIKVMTKQKQLYANYSGGKSK