Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025916.t1 | XP_003626559.1 | 89 | 145 | 9 | 1 | 1 | 145 | 1 | 138 | 4.60E-63 | 250.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025916.t1 | Q8H0Y8 | 44.7 | 161 | 60 | 9 | 8 | 145 | 7 | 161 | 6.5e-18 | 92.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025916.t1 | G7L427 | 89.0 | 145 | 9 | 1 | 1 | 145 | 1 | 138 | 3.3e-63 | 250.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene025916.t1 | TF | WRKY |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025916.t1 | MTR_7g117200 | 88.966 | 145 | 9 | 1 | 1 | 145 | 1 | 138 | 1.24e-87 | 259 |
| MS.gene025916.t1 | MTR_8g027860 | 45.000 | 160 | 69 | 4 | 1 | 145 | 1 | 156 | 2.17e-32 | 118 |
| MS.gene025916.t1 | MTR_3g090860 | 44.079 | 152 | 62 | 9 | 12 | 145 | 7 | 153 | 1.89e-25 | 99.8 |
| MS.gene025916.t1 | MTR_8g099350 | 36.364 | 154 | 87 | 5 | 1 | 145 | 1 | 152 | 1.68e-17 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025916.t1 | AT4G11070 | 43.478 | 161 | 62 | 8 | 8 | 145 | 7 | 161 | 9.37e-24 | 95.1 |
| MS.gene025916.t1 | AT5G24110 | 40.602 | 133 | 61 | 5 | 14 | 145 | 18 | 133 | 2.45e-20 | 85.9 |
| MS.gene025916.t1 | AT4G11070 | 41.135 | 141 | 62 | 6 | 8 | 145 | 7 | 129 | 9.57e-20 | 84.0 |
| MS.gene025916.t1 | AT4G23810 | 34.091 | 176 | 65 | 7 | 11 | 145 | 13 | 178 | 4.85e-19 | 82.4 |
| MS.gene025916.t1 | AT2G46400 | 38.519 | 135 | 64 | 4 | 12 | 145 | 8 | 124 | 5.54e-13 | 65.5 |
Find 31 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTATGATGACCTCACTTT+TGG | 0.188414 | 7.1:-976949 | None:intergenic |
| TCAACATAGTAAGTGCTTTC+TGG | 0.207919 | 7.1:-976851 | None:intergenic |
| CAAGAATTCATTGGTTTCAT+TGG | 0.239108 | 7.1:-976805 | None:intergenic |
| TTCACTTGTTCCTTCCATTT+AGG | 0.269402 | 7.1:-977306 | None:intergenic |
| CATTGGATGATATAATTATA+TGG | 0.275590 | 7.1:-976788 | None:intergenic |
| AGGATTATCTGAAGGGTCTT+TGG | 0.285031 | 7.1:+977344 | MS.gene025916:CDS |
| CTTTCCTTGGATTAATTCAT+TGG | 0.302740 | 7.1:-976742 | None:intergenic |
| GCAGAAAGAGCATGCCTAAA+TGG | 0.338464 | 7.1:+977292 | MS.gene025916:intron |
| AGAAGACAAAGTGCTTATTA+AGG | 0.359138 | 7.1:+977784 | MS.gene025916:CDS |
| TATGGTCAAAAGGATATTCT+TGG | 0.360560 | 7.1:+977390 | MS.gene025916:CDS |
| GAATATAGCTGGAGAAAATA+TGG | 0.367378 | 7.1:+977372 | MS.gene025916:CDS |
| TTTGTCAATCAAGAATTCAT+TGG | 0.407531 | 7.1:-976814 | None:intergenic |
| GCTTTGCTAACTCCTTTCCT+TGG | 0.408135 | 7.1:-976755 | None:intergenic |
| GGACTCTCGTTTGTAAAAGA+AGG | 0.431784 | 7.1:-976927 | None:intergenic |
| CTGGAGAAAATATGGTCAAA+AGG | 0.471109 | 7.1:+977380 | MS.gene025916:CDS |
| GGAGAGACTAAGACCATTGA+TGG | 0.480847 | 7.1:+976885 | MS.gene025916:CDS |
| GACCATTGATGGTAAAATGA+AGG | 0.490394 | 7.1:+976896 | MS.gene025916:CDS |
| ACAATGCTCTCTTCAAGAAA+AGG | 0.501466 | 7.1:+976985 | MS.gene025916:CDS |
| CTTTGGATGATGAATATAGC+TGG | 0.515960 | 7.1:+977361 | MS.gene025916:CDS |
| GAGCATTGTGGTTAAACTCC+TGG | 0.533299 | 7.1:-976971 | None:intergenic |
| CATAGTAAGTGCTTTCTGGT+AGG | 0.575281 | 7.1:-976847 | None:intergenic |
| CAATGAATTAATCCAAGGAA+AGG | 0.585373 | 7.1:+976743 | MS.gene025916:CDS |
| CTTACTATGTTGAATGTGAG+AGG | 0.591736 | 7.1:+976861 | MS.gene025916:CDS |
| AAAGAGCATGCCTAAATGGA+AGG | 0.601574 | 7.1:+977296 | MS.gene025916:CDS |
| GTGAAGATTTGCACAAACAC+AGG | 0.602509 | 7.1:+977324 | MS.gene025916:CDS |
| ATAACCAATGAATTAATCCA+AGG | 0.604601 | 7.1:+976738 | MS.gene025916:CDS |
| ACAAACACAGGATTATCTGA+AGG | 0.625241 | 7.1:+977336 | MS.gene025916:CDS |
| AAGTGAGGTCATCATAGACC+AGG | 0.627405 | 7.1:+976953 | MS.gene025916:CDS |
| CAAACACAGGATTATCTGAA+GGG | 0.645284 | 7.1:+977337 | MS.gene025916:CDS |
| AAACGAGAGTCCCAAAAGTG+AGG | 0.654222 | 7.1:+976938 | MS.gene025916:CDS |
| ACTATGTTGAATGTGAGAGG+AGG | 0.670945 | 7.1:+976864 | MS.gene025916:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTTTTTTGTTAAATATTTA+AGG | + | chr7.1:977665-977684 | MS.gene025916:intron | 10.0% |
| !!! | ATTAATAATATTTGTTTTAG+TGG | + | chr7.1:977490-977509 | MS.gene025916:intron | 10.0% |
| !! | AAATGATACTTGTAATAAAA+CGG | + | chr7.1:977161-977180 | MS.gene025916:intron | 15.0% |
| !! | CTATGTTAATAAAAAAGTTA+AGG | - | chr7.1:977084-977103 | None:intergenic | 15.0% |
| !! | CATTGGATGATATAATTATA+TGG | - | chr7.1:976791-976810 | None:intergenic | 20.0% |
| !! | TATGTAATCATTTGAATTTG+TGG | + | chr7.1:977268-977287 | MS.gene025916:intron | 20.0% |
| !!! | ATATTGCTCAACTATTTTTT+TGG | + | chr7.1:977626-977645 | MS.gene025916:intron | 20.0% |
| !!! | TGCACTATTTGTTAATAATA+AGG | + | chr7.1:977735-977754 | MS.gene025916:intron | 20.0% |
| ! | ATAACCAATGAATTAATCCA+AGG | + | chr7.1:976738-976757 | MS.gene025916:CDS | 25.0% |
| ! | ATCTTGAGTAATGAAATAGA+AGG | - | chr7.1:977714-977733 | None:intergenic | 25.0% |
| ! | TTTGTCAATCAAGAATTCAT+TGG | - | chr7.1:976817-976836 | None:intergenic | 25.0% |
| !! | ATAGTTGAGCAATATTGATT+TGG | - | chr7.1:977621-977640 | None:intergenic | 25.0% |
| !!! | AAGCGTTTTGAATTTGATAT+AGG | + | chr7.1:977528-977547 | MS.gene025916:intron | 25.0% |
| !!! | CAATATTGATTTGGTTAACA+GGG | - | chr7.1:977612-977631 | None:intergenic | 25.0% |
| CAATGAATTAATCCAAGGAA+AGG | + | chr7.1:976743-976762 | MS.gene025916:CDS | 30.0% | |
| CTTTCCTTGGATTAATTCAT+TGG | - | chr7.1:976745-976764 | None:intergenic | 30.0% | |
| GAATATAGCTGGAGAAAATA+TGG | + | chr7.1:977372-977391 | MS.gene025916:CDS | 30.0% | |
| TATGGTCAAAAGGATATTCT+TGG | + | chr7.1:977390-977409 | MS.gene025916:CDS | 30.0% | |
| ! | AGAAGACAAAGTGCTTATTA+AGG | + | chr7.1:977784-977803 | MS.gene025916:CDS | 30.0% |
| ! | ATCCTTCATTTTACCATCAA+TGG | - | chr7.1:976901-976920 | None:intergenic | 30.0% |
| !! | CAAGAATTCATTGGTTTCAT+TGG | - | chr7.1:976808-976827 | None:intergenic | 30.0% |
| !! | GCAATATTGATTTGGTTAAC+AGG | - | chr7.1:977613-977632 | None:intergenic | 30.0% |
| ACAAACACAGGATTATCTGA+AGG | + | chr7.1:977336-977355 | MS.gene025916:CDS | 35.0% | |
| ACAATGCTCTCTTCAAGAAA+AGG | + | chr7.1:976985-977004 | MS.gene025916:CDS | 35.0% | |
| ACTGAAATACGTTCAAGCTA+GGG | - | chr7.1:977249-977268 | None:intergenic | 35.0% | |
| CAAACACAGGATTATCTGAA+GGG | + | chr7.1:977337-977356 | MS.gene025916:CDS | 35.0% | |
| CTGGAGAAAATATGGTCAAA+AGG | + | chr7.1:977380-977399 | MS.gene025916:CDS | 35.0% | |
| CTTACTATGTTGAATGTGAG+AGG | + | chr7.1:976861-976880 | MS.gene025916:CDS | 35.0% | |
| CTTTGGATGATGAATATAGC+TGG | + | chr7.1:977361-977380 | MS.gene025916:CDS | 35.0% | |
| TACTGAAATACGTTCAAGCT+AGG | - | chr7.1:977250-977269 | None:intergenic | 35.0% | |
| TCAACATAGTAAGTGCTTTC+TGG | - | chr7.1:976854-976873 | None:intergenic | 35.0% | |
| TTCACTTGTTCCTTCCATTT+AGG | - | chr7.1:977309-977328 | None:intergenic | 35.0% | |
| TTCATGTGTGTGAATTGTCA+AGG | + | chr7.1:977217-977236 | MS.gene025916:intron | 35.0% | |
| ! | GACCATTGATGGTAAAATGA+AGG | + | chr7.1:976896-976915 | MS.gene025916:CDS | 35.0% |
| ! | TTCTTGGAGCTAAGTTTTCA+AGG | + | chr7.1:977406-977425 | MS.gene025916:CDS | 35.0% |
| !! | TCTATGATGACCTCACTTTT+GGG | - | chr7.1:976951-976970 | None:intergenic | 35.0% |
| !!! | TTTTCTTGAAGAGAGCATTG+TGG | - | chr7.1:976986-977005 | None:intergenic | 35.0% |
| ACTATGTTGAATGTGAGAGG+AGG | + | chr7.1:976864-976883 | MS.gene025916:CDS | 40.0% | |
| AGGATTATCTGAAGGGTCTT+TGG | + | chr7.1:977344-977363 | MS.gene025916:CDS | 40.0% | |
| GGACTCTCGTTTGTAAAAGA+AGG | - | chr7.1:976930-976949 | None:intergenic | 40.0% | |
| GGTTAACAGGGCTAAAAACA+GGG | - | chr7.1:977600-977619 | None:intergenic | 40.0% | |
| GTGAAGATTTGCACAAACAC+AGG | + | chr7.1:977324-977343 | MS.gene025916:CDS | 40.0% | |
| TGGTTAACAGGGCTAAAAAC+AGG | - | chr7.1:977601-977620 | None:intergenic | 40.0% | |
| TGTAAGCATCTTAGTGTCAC+AGG | + | chr7.1:977558-977577 | MS.gene025916:intron | 40.0% | |
| TGTGTGAATTGTCAAGGTCA+AGG | + | chr7.1:977223-977242 | MS.gene025916:intron | 40.0% | |
| ! | AAAGAGCATGCCTAAATGGA+AGG | + | chr7.1:977296-977315 | MS.gene025916:CDS | 40.0% |
| ! | CATAGTAAGTGCTTTCTGGT+AGG | - | chr7.1:976850-976869 | None:intergenic | 40.0% |
| ! | GTCTATGATGACCTCACTTT+TGG | - | chr7.1:976952-976971 | None:intergenic | 40.0% |
| AAACGAGAGTCCCAAAAGTG+AGG | + | chr7.1:976938-976957 | MS.gene025916:CDS | 45.0% | |
| AAGTGAGGTCATCATAGACC+AGG | + | chr7.1:976953-976972 | MS.gene025916:CDS | 45.0% | |
| GCTTTGCTAACTCCTTTCCT+TGG | - | chr7.1:976758-976777 | None:intergenic | 45.0% | |
| GGAGAGACTAAGACCATTGA+TGG | + | chr7.1:976885-976904 | MS.gene025916:CDS | 45.0% | |
| ! | GAGCATTGTGGTTAAACTCC+TGG | - | chr7.1:976974-976993 | None:intergenic | 45.0% |
| ! | GCAGAAAGAGCATGCCTAAA+TGG | + | chr7.1:977292-977311 | MS.gene025916:intron | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 976705 | 977823 | 976705 | ID=MS.gene025916 |
| chr7.1 | mRNA | 976705 | 977823 | 976705 | ID=MS.gene025916.t1;Parent=MS.gene025916 |
| chr7.1 | exon | 976705 | 977006 | 976705 | ID=MS.gene025916.t1.exon1;Parent=MS.gene025916.t1 |
| chr7.1 | CDS | 976705 | 977006 | 976705 | ID=cds.MS.gene025916.t1;Parent=MS.gene025916.t1 |
| chr7.1 | exon | 977296 | 977427 | 977296 | ID=MS.gene025916.t1.exon2;Parent=MS.gene025916.t1 |
| chr7.1 | CDS | 977296 | 977427 | 977296 | ID=cds.MS.gene025916.t1;Parent=MS.gene025916.t1 |
| chr7.1 | exon | 977757 | 977823 | 977757 | ID=MS.gene025916.t1.exon3;Parent=MS.gene025916.t1 |
| chr7.1 | CDS | 977757 | 977823 | 977757 | ID=cds.MS.gene025916.t1;Parent=MS.gene025916.t1 |
| Gene Sequence |
| Protein sequence |
