Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene02594.t1 | XP_013461323.1 | 100 | 151 | 0 | 0 | 1 | 151 | 103 | 253 | 2.30E-77 | 298.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene02594.t1 | Q6DBN1 | 73.5 | 147 | 39 | 0 | 1 | 147 | 88 | 234 | 3.8e-57 | 222.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene02594.t1 | I3T836 | 100.0 | 151 | 0 | 0 | 1 | 151 | 103 | 253 | 1.6e-77 | 298.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene02594.t1 | TR | TRAF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene02594.t1 | MTR_3g088660 | 100.000 | 151 | 0 | 0 | 1 | 151 | 103 | 253 | 1.96e-109 | 311 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene02594.t1 | AT4G08455 | 73.469 | 147 | 39 | 0 | 1 | 147 | 115 | 261 | 3.83e-79 | 235 |
| MS.gene02594.t1 | AT1G01640 | 28.667 | 150 | 106 | 1 | 2 | 150 | 25 | 174 | 2.40e-13 | 64.7 |
| MS.gene02594.t1 | AT4G37610 | 33.113 | 151 | 89 | 4 | 4 | 144 | 78 | 226 | 3.52e-13 | 65.9 |
| MS.gene02594.t1 | AT1G01640 | 29.496 | 139 | 97 | 1 | 2 | 139 | 46 | 184 | 3.65e-13 | 64.3 |
| MS.gene02594.t1 | AT2G05330 | 30.065 | 153 | 101 | 2 | 2 | 148 | 37 | 189 | 2.26e-11 | 59.7 |
Find 32 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTAAAACTAATAGGTTAC+AGG | 0.263882 | 3.3:-74501196 | None:intergenic |
| ATTTGATCTCCAAACTGAAT+TGG | 0.305803 | 3.3:+74501262 | MS.gene02594:CDS |
| TGCAGCCACGCTAGGGTTAT+AGG | 0.318170 | 3.3:+74501476 | MS.gene02594:CDS |
| TCAAGCATGGCTCTGAAAAC+TGG | 0.323355 | 3.3:-74501054 | None:intergenic |
| GGCAGTGATCATGGAGAATA+TGG | 0.334507 | 3.3:+74501353 | MS.gene02594:CDS |
| CTAATAGGTTACAGGCCATT+TGG | 0.355315 | 3.3:-74501188 | None:intergenic |
| AGCTTTCTCCCAATTCAGTT+TGG | 0.363821 | 3.3:-74501271 | None:intergenic |
| TGAAGCTTATCTTGCCAAAC+AGG | 0.369315 | 3.3:+74501446 | MS.gene02594:CDS |
| CCAATTGCAAGATGCAGCTT+TGG | 0.375309 | 3.3:+74501332 | MS.gene02594:CDS |
| GGCTGCAGTATTAACCTGTT+TGG | 0.400932 | 3.3:-74501460 | None:intergenic |
| TTTGATCTCCAAACTGAATT+GGG | 0.405372 | 3.3:+74501263 | MS.gene02594:CDS |
| CCAAAGCTGCATCTTGCAAT+TGG | 0.405862 | 3.3:-74501332 | None:intergenic |
| TACAGGCCATTTGGTTATCA+AGG | 0.418138 | 3.3:-74501179 | None:intergenic |
| CGAAACATCAGCAATCTTGA+TGG | 0.418642 | 3.3:-74501106 | None:intergenic |
| TGCAGCTTTGGCAGTGATCA+TGG | 0.425358 | 3.3:+74501344 | MS.gene02594:CDS |
| AGCCATGCTTGAGAATGATA+TGG | 0.435569 | 3.3:+74501065 | MS.gene02594:CDS |
| GTTAATACTGCAGCCACGCT+AGG | 0.472280 | 3.3:+74501468 | MS.gene02594:CDS |
| AGCTGCATCTTGCAATTGGT+TGG | 0.493566 | 3.3:-74501328 | None:intergenic |
| AACTAATCCTCGTTTAGTTG+TGG | 0.493649 | 3.3:+74501416 | MS.gene02594:CDS |
| GTATCAGGTGAAGCATCTTA+AGG | 0.500142 | 3.3:+74501224 | MS.gene02594:CDS |
| CTCCATATCATTCTCAAGCA+TGG | 0.507732 | 3.3:-74501067 | None:intergenic |
| TGAAGATTACACGGAGTTGG+TGG | 0.522112 | 3.3:+74501392 | MS.gene02594:CDS |
| AAATGAAGATTACACGGAGT+TGG | 0.527013 | 3.3:+74501389 | MS.gene02594:CDS |
| AGCATGCCTTGATAACCAAA+TGG | 0.551457 | 3.3:+74501173 | MS.gene02594:CDS |
| TTAATACTGCAGCCACGCTA+GGG | 0.570249 | 3.3:+74501469 | MS.gene02594:CDS |
| AGAATGATATGGAGGAAAGC+CGG | 0.570838 | 3.3:+74501076 | MS.gene02594:CDS |
| GGCATTGTGTTGATGTGCAA+AGG | 0.583175 | 3.3:-74501307 | None:intergenic |
| CAATCTTGATGGTGCCACTC+CGG | 0.589211 | 3.3:-74501095 | None:intergenic |
| CATGCTTGAGAATGATATGG+AGG | 0.646700 | 3.3:+74501068 | MS.gene02594:CDS |
| TATATTTCCACAACTAAACG+AGG | 0.674137 | 3.3:-74501423 | None:intergenic |
| GATATGGAGGAAAGCCGGAG+TGG | 0.686483 | 3.3:+74501081 | MS.gene02594:CDS |
| GCATTCCTATAACCCTAGCG+TGG | 0.731788 | 3.3:-74501481 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CACTAAAAATGAAGATTACA+CGG | + | chr3.3:74501383-74501402 | MS.gene02594:CDS | 25.0% |
| !! | ACTTTTCTCCTAAAACTAAT+AGG | - | chr3.3:74501206-74501225 | None:intergenic | 25.0% |
| !!! | TGTAATCTTCATTTTTAGTG+AGG | - | chr3.3:74501383-74501402 | None:intergenic | 25.0% |
| ATTTGATCTCCAAACTGAAT+TGG | + | chr3.3:74501262-74501281 | MS.gene02594:CDS | 30.0% | |
| TATATTTCCACAACTAAACG+AGG | - | chr3.3:74501426-74501445 | None:intergenic | 30.0% | |
| TCCTAAAACTAATAGGTTAC+AGG | - | chr3.3:74501199-74501218 | None:intergenic | 30.0% | |
| TTTGATCTCCAAACTGAATT+GGG | + | chr3.3:74501263-74501282 | MS.gene02594:CDS | 30.0% | |
| !!! | AGTTTTAGGAGAAAAGTATC+AGG | + | chr3.3:74501209-74501228 | MS.gene02594:CDS | 30.0% |
| AAATGAAGATTACACGGAGT+TGG | + | chr3.3:74501389-74501408 | MS.gene02594:CDS | 35.0% | |
| AACTAATCCTCGTTTAGTTG+TGG | + | chr3.3:74501416-74501435 | MS.gene02594:CDS | 35.0% | |
| !!! | GCCTGTAACCTATTAGTTTT+AGG | + | chr3.3:74501195-74501214 | MS.gene02594:CDS | 35.0% |
| AGAATGATATGGAGGAAAGC+CGG | + | chr3.3:74501076-74501095 | MS.gene02594:CDS | 40.0% | |
| AGCCATGCTTGAGAATGATA+TGG | + | chr3.3:74501065-74501084 | MS.gene02594:CDS | 40.0% | |
| AGCTTTCTCCCAATTCAGTT+TGG | - | chr3.3:74501274-74501293 | None:intergenic | 40.0% | |
| CATGCTTGAGAATGATATGG+AGG | + | chr3.3:74501068-74501087 | MS.gene02594:CDS | 40.0% | |
| CGAAACATCAGCAATCTTGA+TGG | - | chr3.3:74501109-74501128 | None:intergenic | 40.0% | |
| CTAATAGGTTACAGGCCATT+TGG | - | chr3.3:74501191-74501210 | None:intergenic | 40.0% | |
| CTCCATATCATTCTCAAGCA+TGG | - | chr3.3:74501070-74501089 | None:intergenic | 40.0% | |
| TACAGGCCATTTGGTTATCA+AGG | - | chr3.3:74501182-74501201 | None:intergenic | 40.0% | |
| TGAAGCTTATCTTGCCAAAC+AGG | + | chr3.3:74501446-74501465 | MS.gene02594:CDS | 40.0% | |
| ! | AGCATGCCTTGATAACCAAA+TGG | + | chr3.3:74501173-74501192 | MS.gene02594:CDS | 40.0% |
| ! | GTATCAGGTGAAGCATCTTA+AGG | + | chr3.3:74501224-74501243 | MS.gene02594:CDS | 40.0% |
| AGCTGCATCTTGCAATTGGT+TGG | - | chr3.3:74501331-74501350 | None:intergenic | 45.0% | |
| CCAAAGCTGCATCTTGCAAT+TGG | - | chr3.3:74501335-74501354 | None:intergenic | 45.0% | |
| CCAATTGCAAGATGCAGCTT+TGG | + | chr3.3:74501332-74501351 | MS.gene02594:CDS | 45.0% | |
| GGCAGTGATCATGGAGAATA+TGG | + | chr3.3:74501353-74501372 | MS.gene02594:CDS | 45.0% | |
| GGCTGCAGTATTAACCTGTT+TGG | - | chr3.3:74501463-74501482 | None:intergenic | 45.0% | |
| TGAAGATTACACGGAGTTGG+TGG | + | chr3.3:74501392-74501411 | MS.gene02594:CDS | 45.0% | |
| TTAATACTGCAGCCACGCTA+GGG | + | chr3.3:74501469-74501488 | MS.gene02594:CDS | 45.0% | |
| ! | GGCATTGTGTTGATGTGCAA+AGG | - | chr3.3:74501310-74501329 | None:intergenic | 45.0% |
| !! | TCAAGCATGGCTCTGAAAAC+TGG | - | chr3.3:74501057-74501076 | None:intergenic | 45.0% |
| GTTAATACTGCAGCCACGCT+AGG | + | chr3.3:74501468-74501487 | MS.gene02594:CDS | 50.0% | |
| ! | TGCAGCTTTGGCAGTGATCA+TGG | + | chr3.3:74501344-74501363 | MS.gene02594:CDS | 50.0% |
| !! | CAATCTTGATGGTGCCACTC+CGG | - | chr3.3:74501098-74501117 | None:intergenic | 50.0% |
| GATATGGAGGAAAGCCGGAG+TGG | + | chr3.3:74501081-74501100 | MS.gene02594:CDS | 55.0% | |
| TGCAGCCACGCTAGGGTTAT+AGG | + | chr3.3:74501476-74501495 | MS.gene02594:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 74501042 | 74501497 | 74501042 | ID=MS.gene02594 |
| chr3.3 | mRNA | 74501042 | 74501497 | 74501042 | ID=MS.gene02594.t1;Parent=MS.gene02594 |
| chr3.3 | exon | 74501042 | 74501497 | 74501042 | ID=MS.gene02594.t1.exon1;Parent=MS.gene02594.t1 |
| chr3.3 | CDS | 74501042 | 74501497 | 74501042 | ID=cds.MS.gene02594.t1;Parent=MS.gene02594.t1 |
| Gene Sequence |
| Protein sequence |
