Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026022.t1 | AFK34644.1 | 57.7 | 383 | 137 | 10 | 5 | 371 | 12 | 385 | 2.40E-88 | 336.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026022.t1 | Q99090 | 39.6 | 361 | 192 | 9 | 21 | 362 | 28 | 381 | 2.1e-48 | 194.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026022.t1 | G7L1X5 | 57.7 | 383 | 137 | 10 | 5 | 371 | 12 | 385 | 1.7e-88 | 336.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene026022.t1 | TF | bZIP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026022.t1 | MTR_7g115120 | 59.635 | 384 | 128 | 10 | 5 | 371 | 12 | 385 | 3.37e-117 | 350 |
| MS.gene026022.t1 | MTR_1g080920 | 49.527 | 317 | 131 | 11 | 68 | 362 | 82 | 391 | 2.51e-69 | 227 |
| MS.gene026022.t1 | MTR_7g115140 | 76.471 | 153 | 21 | 4 | 100 | 250 | 67 | 206 | 1.35e-65 | 211 |
| MS.gene026022.t1 | MTR_7g115180 | 96.341 | 82 | 3 | 0 | 405 | 486 | 63 | 144 | 7.92e-45 | 154 |
| MS.gene026022.t1 | MTR_1g080910 | 71.951 | 82 | 18 | 1 | 405 | 486 | 63 | 139 | 3.82e-30 | 114 |
| MS.gene026022.t1 | MTR_7g115180 | 72.414 | 58 | 16 | 0 | 405 | 462 | 63 | 120 | 3.38e-19 | 83.6 |
| MS.gene026022.t1 | MTR_1g080910 | 94.737 | 38 | 2 | 0 | 405 | 442 | 63 | 100 | 3.20e-17 | 77.8 |
| MS.gene026022.t1 | MTR_1g087050 | 77.778 | 45 | 9 | 1 | 442 | 486 | 88 | 131 | 1.66e-12 | 65.1 |
| MS.gene026022.t1 | MTR_3g109310 | 36.296 | 135 | 77 | 4 | 131 | 259 | 76 | 207 | 3.24e-12 | 67.8 |
| MS.gene026022.t1 | MTR_1g008990 | 42.500 | 120 | 66 | 2 | 150 | 268 | 142 | 259 | 5.36e-12 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026022.t1 | AT5G28770 | 39.700 | 267 | 109 | 11 | 17 | 271 | 20 | 246 | 2.06e-29 | 117 |
| MS.gene026022.t1 | AT5G28770 | 39.245 | 265 | 106 | 10 | 17 | 271 | 20 | 239 | 6.02e-29 | 116 |
| MS.gene026022.t1 | AT5G28750 | 61.053 | 95 | 35 | 1 | 394 | 486 | 53 | 147 | 1.45e-27 | 108 |
| MS.gene026022.t1 | AT5G28770 | 38.618 | 246 | 99 | 11 | 17 | 250 | 20 | 225 | 2.10e-22 | 96.7 |
| MS.gene026022.t1 | AT4G02640 | 39.738 | 229 | 112 | 9 | 47 | 267 | 102 | 312 | 5.59e-22 | 98.2 |
| MS.gene026022.t1 | AT4G02640 | 39.738 | 229 | 112 | 9 | 47 | 267 | 102 | 312 | 6.19e-22 | 98.2 |
| MS.gene026022.t1 | AT4G02640 | 39.823 | 226 | 110 | 8 | 47 | 267 | 102 | 306 | 7.60e-22 | 97.8 |
| MS.gene026022.t1 | AT4G02640 | 39.823 | 226 | 110 | 8 | 47 | 267 | 102 | 306 | 9.70e-22 | 97.8 |
| MS.gene026022.t1 | AT5G24800 | 42.069 | 145 | 66 | 5 | 133 | 264 | 71 | 210 | 1.42e-20 | 92.0 |
| MS.gene026022.t1 | AT3G54620 | 29.712 | 313 | 147 | 8 | 21 | 282 | 40 | 330 | 3.47e-19 | 90.1 |
Find 103 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTCAGGATGCTCCAAAAT+AGG | 0.198060 | 7.1:-2225793 | MS.gene026022:intron |
| GGACTTCAGCCTACTGTAAA+AGG | 0.236825 | 7.1:-2226202 | MS.gene026022:intron |
| GTTTAGCTGATTCCCTATTT+TGG | 0.286405 | 7.1:+2225780 | None:intergenic |
| TACTGAGGTTTCTAGTTCTA+AGG | 0.292945 | 7.1:-2215677 | MS.gene026022:CDS |
| GTTTGAGTCTGAGCTTAAAA+AGG | 0.305627 | 7.1:-2215767 | MS.gene026022:CDS |
| CCCTTCTGAATTACAAAATA+AGG | 0.310861 | 7.1:-2226039 | MS.gene026022:CDS |
| CAATGGTTATTCCCGTTAAT+AGG | 0.318207 | 7.1:-2219898 | MS.gene026022:CDS |
| GAGAGATGTAAAAGATTAAC+TGG | 0.323266 | 7.1:-2225241 | MS.gene026022:CDS |
| GGATCTGATCCTTCTGATAT+TGG | 0.324824 | 7.1:-2226253 | MS.gene026022:CDS |
| TGTCTAAAGGAAATTGATAA+AGG | 0.341441 | 7.1:-2227673 | MS.gene026022:CDS |
| GGAGAGGTTAATATGAATAT+TGG | 0.345096 | 7.1:-2225912 | MS.gene026022:CDS |
| ACAAAGGTGGAAGAAGTTAA+AGG | 0.357578 | 7.1:+2227622 | None:intergenic |
| AGGGGAAGAGAAAGCTTCTC+AGG | 0.359196 | 7.1:-2225934 | MS.gene026022:CDS |
| CCTGAACTCGTTGTTATTGC+TGG | 0.366575 | 7.1:-2217757 | MS.gene026022:CDS |
| GGCGCATTCAAGATCAAGTT+TGG | 0.367531 | 7.1:+2227528 | None:intergenic |
| GATATGAGAGGAGTCAATAA+TGG | 0.367652 | 7.1:-2225049 | MS.gene026022:CDS |
| TATTAGGTCTCTACATTAAG+AGG | 0.369226 | 7.1:-2225648 | MS.gene026022:intron |
| ACGCACGCACGCGAAGCTTT+CGG | 0.377409 | 7.1:+2219875 | None:intergenic |
| AAGATTAACTGGATCAATTC+CGG | 0.379673 | 7.1:-2225230 | MS.gene026022:CDS |
| AACTGTCAAAAGCTTTCAAC+AGG | 0.389906 | 7.1:-2217674 | MS.gene026022:intron |
| CCGATACTGCGACCAACTTC+AGG | 0.394518 | 7.1:+2217700 | None:intergenic |
| ATTGATGTTGCCTCAGACAT+TGG | 0.396102 | 7.1:+2225193 | None:intergenic |
| GAAGCTTTCGGCCTATTAAC+GGG | 0.398529 | 7.1:+2219887 | None:intergenic |
| GGCTCCCCTCCTGAGGAATC+TGG | 0.401880 | 7.1:+2215742 | None:intergenic |
| TTTCAGGATGCTCCAAAATA+GGG | 0.416451 | 7.1:-2225792 | MS.gene026022:intron |
| CGAAGCTTTCGGCCTATTAA+CGG | 0.421945 | 7.1:+2219886 | None:intergenic |
| ACATCTGGTCCTTCAGAACT+TGG | 0.423513 | 7.1:-2226223 | MS.gene026022:CDS |
| GACATTGGATTATTAAAAGT+TGG | 0.427671 | 7.1:+2225079 | None:intergenic |
| ACATACCCAAGTCATAATAA+AGG | 0.434312 | 7.1:+2227504 | None:intergenic |
| AGAGGAGTCAATAATGGGTT+GGG | 0.448583 | 7.1:-2225043 | MS.gene026022:CDS |
| GCAGCAGGATGGAACGAGAT+AGG | 0.450336 | 7.1:-2224980 | MS.gene026022:CDS |
| TGATGACCAGGCTCTTCTTA+AGG | 0.454270 | 7.1:-2227551 | MS.gene026022:CDS |
| CAAAGCTATCCACCCGCGGC+AGG | 0.455051 | 7.1:+2224945 | None:intergenic |
| CGCCGCCTTTATTATGACTT+GGG | 0.455555 | 7.1:-2227509 | MS.gene026022:intron |
| TGAAACCTGTTGTCACAATC+AGG | 0.455873 | 7.1:-2225977 | MS.gene026022:CDS |
| GACATGATCATAGTTGCTGC+TGG | 0.457615 | 7.1:-2226017 | MS.gene026022:CDS |
| ATGGGAATGCCATTACTTGA+TGG | 0.462778 | 7.1:-2225172 | MS.gene026022:CDS |
| AATGTCTGAGGCAACATCAA+TGG | 0.467879 | 7.1:-2225191 | MS.gene026022:CDS |
| GTCTAAAGGAAATTGATAAA+GGG | 0.468058 | 7.1:-2227672 | MS.gene026022:CDS |
| AAAGCTTCGCGTGCGTGCGT+CGG | 0.471695 | 7.1:-2219872 | MS.gene026022:intron |
| GAGAGGAGTCAATAATGGGT+TGG | 0.473171 | 7.1:-2225044 | MS.gene026022:CDS |
| AAGGAGAAACAAAGATGAAT+CGG | 0.475504 | 7.1:-2227723 | MS.gene026022:CDS |
| ATGTCTGAGGCAACATCAAT+GGG | 0.480461 | 7.1:-2225190 | MS.gene026022:CDS |
| CCAGCAATAACAACGAGTTC+AGG | 0.481286 | 7.1:+2217757 | None:intergenic |
| TCAACGACAAGTGGATCCTC+AGG | 0.483871 | 7.1:-2225954 | MS.gene026022:CDS |
| GCGCCGCCTTTATTATGACT+TGG | 0.484716 | 7.1:-2227510 | MS.gene026022:CDS |
| ATATGAGAGGAGTCAATAAT+GGG | 0.486438 | 7.1:-2225048 | MS.gene026022:CDS |
| GATATTGGACGACAGACATC+TGG | 0.495965 | 7.1:-2226238 | MS.gene026022:CDS |
| TTATTTGGATCTTCTTGCAC+AGG | 0.496736 | 7.1:+2225115 | None:intergenic |
| GGTGAAAAGAATGTCATCAA+TGG | 0.497878 | 7.1:-2227779 | None:intergenic |
| TTTGAGATTGTTATTAACAA+AGG | 0.498642 | 7.1:+2227606 | None:intergenic |
| CCTGAAGTTGGTCGCAGTAT+CGG | 0.502726 | 7.1:-2217700 | MS.gene026022:CDS |
| GTTGACCTGATTGTGACAAC+AGG | 0.513826 | 7.1:+2225972 | None:intergenic |
| GGATAGCTTTGATCATCAGC+TGG | 0.514472 | 7.1:-2224933 | MS.gene026022:CDS |
| ACAGGTTTCAGTGGCATGTA+GGG | 0.514510 | 7.1:+2225990 | None:intergenic |
| GTGCAGAAGAACACTGCAGC+AGG | 0.518310 | 7.1:-2224995 | MS.gene026022:CDS |
| GGAACAATATTTGTTGATGT+CGG | 0.520331 | 7.1:-2227758 | MS.gene026022:CDS |
| AACAAATTCCCTGCCGCGGG+TGG | 0.522410 | 7.1:-2224954 | MS.gene026022:CDS |
| GTAAATCATGTAGGCAGCAA+AGG | 0.522582 | 7.1:-2215791 | MS.gene026022:intron |
| ACAGCAATTGATTTCTCAGA+TGG | 0.525895 | 7.1:+2215721 | None:intergenic |
| CGACGGCGGCGAAGGGGCAA+TGG | 0.532985 | 7.1:-2219915 | MS.gene026022:CDS |
| ATTGGCATTGCATTAAATGC+CGG | 0.535991 | 7.1:+2225211 | None:intergenic |
| GACTCCTCTCATATCATGAC+TGG | 0.537853 | 7.1:+2225057 | None:intergenic |
| CTCATATCATGACTGGACAT+TGG | 0.537888 | 7.1:+2225064 | None:intergenic |
| GAGATTGTTATTAACAAAGG+TGG | 0.550741 | 7.1:+2227609 | None:intergenic |
| ACATGATCATAGTTGCTGCT+GGG | 0.551656 | 7.1:-2226016 | MS.gene026022:CDS |
| GTTTGGCCTTAAGAAGAGCC+TGG | 0.554569 | 7.1:+2227545 | None:intergenic |
| CTGATGCAAAACGAGTTAAG+AGG | 0.557366 | 7.1:-2225878 | MS.gene026022:intron |
| AGAGAAAGCTTCTCAGGGAG+AGG | 0.567096 | 7.1:-2225928 | MS.gene026022:CDS |
| AAGAACAAATTCCCTGCCGC+GGG | 0.569746 | 7.1:-2224957 | MS.gene026022:CDS |
| AACAGGTTTCAGTGGCATGT+AGG | 0.571055 | 7.1:+2225989 | None:intergenic |
| CTCAGATGGCTCCCCTCCTG+AGG | 0.577964 | 7.1:+2215735 | None:intergenic |
| ACAATCAGGTCAACGACAAG+TGG | 0.581189 | 7.1:-2225963 | MS.gene026022:CDS |
| TTTCGTCGACGGCGGCGAAG+GGG | 0.588349 | 7.1:-2219921 | MS.gene026022:CDS |
| GTCTGTCGTCCAATATCAGA+AGG | 0.588926 | 7.1:+2226244 | None:intergenic |
| GAAGGACTTCCATCAAGTAA+TGG | 0.591255 | 7.1:+2225163 | None:intergenic |
| TAATGCAATGCCAATGTCTG+AGG | 0.599128 | 7.1:-2225203 | MS.gene026022:CDS |
| AAGGTGGAAGAAGTTAAAGG+TGG | 0.599798 | 7.1:+2227625 | None:intergenic |
| GGCTGAAGTCCAAGTTCTGA+AGG | 0.600351 | 7.1:+2226214 | None:intergenic |
| AAAGCTATCCACCCGCGGCA+GGG | 0.601789 | 7.1:+2224946 | None:intergenic |
| GGGGAAGAGAAAGCTTCTCA+GGG | 0.602774 | 7.1:-2225933 | MS.gene026022:CDS |
| CATGATCATAGTTGCTGCTG+GGG | 0.605839 | 7.1:-2226015 | MS.gene026022:CDS |
| TTTAAAGAAAGATGATGACC+AGG | 0.607833 | 7.1:-2227563 | MS.gene026022:CDS |
| GGAACCAGATTCCTCAGGAG+GGG | 0.609899 | 7.1:-2215746 | MS.gene026022:CDS |
| AAGGAACCAGATTCCTCAGG+AGG | 0.613287 | 7.1:-2215748 | MS.gene026022:CDS |
| AAATACTAAGCTTGAAACAC+AGG | 0.618412 | 7.1:-2225732 | MS.gene026022:intron |
| AGGAACCAGATTCCTCAGGA+GGG | 0.620434 | 7.1:-2215747 | MS.gene026022:CDS |
| GCATCAGCTGATAACTCAGA+AGG | 0.620964 | 7.1:+2225145 | None:intergenic |
| TGGATCCATAGGCTGGTGGT+TGG | 0.621385 | 7.1:-2219948 | MS.gene026022:intron |
| CAACGACAAGTGGATCCTCA+GGG | 0.625807 | 7.1:-2225953 | MS.gene026022:CDS |
| ATTGTGACAACAGGTTTCAG+TGG | 0.628600 | 7.1:+2225981 | None:intergenic |
| GGAGTCAATAATGGGTTGGG+AGG | 0.632977 | 7.1:-2225040 | MS.gene026022:CDS |
| ATTTGTTGATGTCGGATACG+AGG | 0.633519 | 7.1:-2227750 | MS.gene026022:CDS |
| TCGCTGGATCCATAGGCTGG+TGG | 0.642076 | 7.1:-2219952 | MS.gene026022:intron |
| AGAGAAGAAGCAAGATACTG+AGG | 0.643616 | 7.1:-2215692 | MS.gene026022:CDS |
| ATGTCGGATACGAGGAACGA+AGG | 0.644161 | 7.1:-2227742 | MS.gene026022:CDS |
| GAAGAACAAATTCCCTGCCG+CGG | 0.646610 | 7.1:-2224958 | MS.gene026022:CDS |
| AGAAGCTTTCTCTTCCCCTG+AGG | 0.650082 | 7.1:+2225938 | None:intergenic |
| ATGTCCAGTCATGATATGAG+AGG | 0.664118 | 7.1:-2225061 | MS.gene026022:CDS |
| TACCCAAGTCATAATAAAGG+CGG | 0.666788 | 7.1:+2227507 | None:intergenic |
| AGAAGAACACTGCAGCAGGA+TGG | 0.669756 | 7.1:-2224991 | MS.gene026022:CDS |
| AACGACAAGTGGATCCTCAG+GGG | 0.710138 | 7.1:-2225952 | MS.gene026022:CDS |
| TGATCAAAGCTATCCACCCG+CGG | 0.714097 | 7.1:+2224941 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 2215666 | 2227791 | 2215666 | ID=MS.gene026022 |
| chr7.1 | mRNA | 2215666 | 2227791 | 2215666 | ID=MS.gene026022.t1;Parent=MS.gene026022 |
| chr7.1 | exon | 2227510 | 2227791 | 2227510 | ID=MS.gene026022.t1.exon1;Parent=MS.gene026022.t1 |
| chr7.1 | CDS | 2227510 | 2227791 | 2227510 | ID=cds.MS.gene026022.t1;Parent=MS.gene026022.t1 |
| chr7.1 | exon | 2226203 | 2226275 | 2226203 | ID=MS.gene026022.t1.exon2;Parent=MS.gene026022.t1 |
| chr7.1 | CDS | 2226203 | 2226275 | 2226203 | ID=cds.MS.gene026022.t1;Parent=MS.gene026022.t1 |
| chr7.1 | exon | 2225879 | 2226068 | 2225879 | ID=MS.gene026022.t1.exon3;Parent=MS.gene026022.t1 |
| chr7.1 | CDS | 2225879 | 2226068 | 2225879 | ID=cds.MS.gene026022.t1;Parent=MS.gene026022.t1 |
| chr7.1 | exon | 2225733 | 2225808 | 2225733 | ID=MS.gene026022.t1.exon4;Parent=MS.gene026022.t1 |
| chr7.1 | CDS | 2225733 | 2225808 | 2225733 | ID=cds.MS.gene026022.t1;Parent=MS.gene026022.t1 |
| chr7.1 | exon | 2225539 | 2225664 | 2225539 | ID=MS.gene026022.t1.exon5;Parent=MS.gene026022.t1 |
| chr7.1 | CDS | 2225539 | 2225664 | 2225539 | ID=cds.MS.gene026022.t1;Parent=MS.gene026022.t1 |
| chr7.1 | exon | 2224897 | 2225278 | 2224897 | ID=MS.gene026022.t1.exon6;Parent=MS.gene026022.t1 |
| chr7.1 | CDS | 2224897 | 2225278 | 2224897 | ID=cds.MS.gene026022.t1;Parent=MS.gene026022.t1 |
| chr7.1 | exon | 2219873 | 2219959 | 2219873 | ID=MS.gene026022.t1.exon7;Parent=MS.gene026022.t1 |
| chr7.1 | CDS | 2219873 | 2219959 | 2219873 | ID=cds.MS.gene026022.t1;Parent=MS.gene026022.t1 |
| chr7.1 | exon | 2217675 | 2217784 | 2217675 | ID=MS.gene026022.t1.exon8;Parent=MS.gene026022.t1 |
| chr7.1 | CDS | 2217675 | 2217784 | 2217675 | ID=cds.MS.gene026022.t1;Parent=MS.gene026022.t1 |
| chr7.1 | exon | 2215666 | 2215800 | 2215666 | ID=MS.gene026022.t1.exon9;Parent=MS.gene026022.t1 |
| chr7.1 | CDS | 2215666 | 2215800 | 2215666 | ID=cds.MS.gene026022.t1;Parent=MS.gene026022.t1 |
| Gene Sequence |
| Protein sequence |
