Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026129.t1 | RHN78466.1 | 92 | 100 | 5 | 1 | 1 | 97 | 575 | 674 | 8.10E-44 | 186 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026129.t1 | Q8VZG8 | 42.6 | 94 | 50 | 1 | 1 | 94 | 955 | 1044 | 5.7e-14 | 78.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026129.t1 | A0A072VHT7 | 92.0 | 100 | 5 | 1 | 1 | 97 | 767 | 866 | 5.8e-44 | 186.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026129.t1 | MTR_1g040525 | 92.000 | 100 | 5 | 1 | 1 | 97 | 767 | 866 | 5.30e-59 | 193 |
| MS.gene026129.t1 | MTR_1g040615 | 90.000 | 100 | 7 | 1 | 1 | 97 | 2024 | 2123 | 1.47e-56 | 187 |
| MS.gene026129.t1 | MTR_1g040615 | 86.957 | 92 | 9 | 1 | 1 | 89 | 1025 | 1116 | 3.51e-49 | 166 |
| MS.gene026129.t1 | MTR_1g040545 | 90.000 | 100 | 7 | 1 | 1 | 97 | 907 | 1006 | 2.97e-56 | 186 |
| MS.gene026129.t1 | MTR_1g039220 | 80.000 | 100 | 17 | 1 | 1 | 97 | 1032 | 1131 | 5.43e-51 | 171 |
| MS.gene026129.t1 | MTR_0400s0040 | 77.000 | 100 | 20 | 1 | 1 | 97 | 1058 | 1157 | 3.04e-48 | 164 |
| MS.gene026129.t1 | MTR_0365s0030 | 77.000 | 100 | 20 | 1 | 1 | 97 | 1058 | 1157 | 3.04e-48 | 164 |
| MS.gene026129.t1 | MTR_1g039240 | 76.000 | 100 | 21 | 1 | 1 | 97 | 1058 | 1157 | 4.66e-48 | 163 |
| MS.gene026129.t1 | MTR_1g040625 | 70.213 | 94 | 23 | 3 | 8 | 97 | 478 | 570 | 3.23e-35 | 125 |
| MS.gene026129.t1 | MTR_8g445960 | 57.000 | 100 | 37 | 2 | 1 | 97 | 63 | 159 | 5.97e-33 | 112 |
| MS.gene026129.t1 | MTR_8g044940 | 57.000 | 100 | 37 | 2 | 1 | 97 | 63 | 159 | 5.97e-33 | 112 |
| MS.gene026129.t1 | MTR_1g038890 | 60.000 | 100 | 34 | 2 | 1 | 97 | 1033 | 1129 | 1.11e-32 | 119 |
| MS.gene026129.t1 | MTR_1g039080 | 58.000 | 100 | 36 | 2 | 1 | 97 | 192 | 288 | 2.87e-32 | 114 |
| MS.gene026129.t1 | MTR_0400s0030 | 60.000 | 100 | 34 | 2 | 1 | 97 | 791 | 887 | 3.67e-32 | 117 |
| MS.gene026129.t1 | MTR_0365s0020 | 60.000 | 100 | 34 | 2 | 1 | 97 | 791 | 887 | 3.67e-32 | 117 |
| MS.gene026129.t1 | MTR_1g039310 | 60.204 | 98 | 33 | 2 | 3 | 97 | 1051 | 1145 | 1.12e-31 | 116 |
| MS.gene026129.t1 | MTR_7g045860 | 56.566 | 99 | 34 | 3 | 2 | 97 | 984 | 1076 | 1.00e-29 | 110 |
| MS.gene026129.t1 | MTR_7g051040 | 53.465 | 101 | 43 | 2 | 1 | 97 | 843 | 943 | 9.90e-29 | 108 |
| MS.gene026129.t1 | MTR_7g050990 | 53.000 | 100 | 44 | 1 | 1 | 97 | 819 | 918 | 3.56e-28 | 106 |
| MS.gene026129.t1 | MTR_7g045710 | 53.763 | 93 | 37 | 2 | 2 | 94 | 972 | 1058 | 2.00e-27 | 104 |
| MS.gene026129.t1 | MTR_7g446160 | 50.515 | 97 | 42 | 2 | 1 | 94 | 887 | 980 | 2.20e-27 | 104 |
| MS.gene026129.t1 | MTR_7g045510 | 54.839 | 93 | 39 | 1 | 2 | 94 | 1091 | 1180 | 1.27e-26 | 102 |
| MS.gene026129.t1 | MTR_7g039330 | 54.839 | 93 | 39 | 1 | 2 | 94 | 1189 | 1278 | 2.33e-26 | 101 |
| MS.gene026129.t1 | MTR_7g081720 | 41.667 | 96 | 55 | 1 | 1 | 96 | 964 | 1058 | 1.79e-23 | 93.2 |
| MS.gene026129.t1 | MTR_7g481630 | 45.000 | 100 | 48 | 2 | 1 | 97 | 80 | 175 | 3.24e-23 | 88.2 |
| MS.gene026129.t1 | MTR_5g085700 | 44.792 | 96 | 52 | 1 | 2 | 97 | 921 | 1015 | 8.63e-23 | 91.3 |
| MS.gene026129.t1 | MTR_7g081570 | 45.455 | 99 | 47 | 2 | 1 | 96 | 939 | 1033 | 1.88e-22 | 90.5 |
| MS.gene026129.t1 | MTR_7g481590 | 45.000 | 100 | 48 | 2 | 1 | 97 | 203 | 298 | 7.31e-22 | 87.0 |
| MS.gene026129.t1 | MTR_7g081480 | 46.316 | 95 | 44 | 3 | 1 | 92 | 949 | 1039 | 1.13e-20 | 85.1 |
| MS.gene026129.t1 | MTR_7g007540 | 44.792 | 96 | 47 | 3 | 1 | 95 | 1 | 91 | 1.17e-20 | 80.1 |
| MS.gene026129.t1 | MTR_4g037015 | 41.667 | 96 | 51 | 1 | 1 | 96 | 777 | 867 | 2.63e-20 | 84.3 |
| MS.gene026129.t1 | MTR_7g081410 | 45.455 | 99 | 47 | 3 | 1 | 96 | 946 | 1040 | 2.66e-20 | 84.3 |
| MS.gene026129.t1 | MTR_7g028432 | 41.667 | 96 | 51 | 1 | 1 | 96 | 853 | 943 | 3.06e-20 | 84.0 |
| MS.gene026129.t1 | MTR_7g081410 | 45.455 | 99 | 47 | 3 | 1 | 96 | 826 | 920 | 3.54e-20 | 84.0 |
| MS.gene026129.t1 | MTR_4g037720 | 41.489 | 94 | 50 | 1 | 1 | 94 | 848 | 936 | 4.09e-20 | 83.6 |
| MS.gene026129.t1 | MTR_7g446190 | 46.429 | 84 | 39 | 2 | 1 | 81 | 815 | 895 | 2.18e-19 | 81.6 |
| MS.gene026129.t1 | MTR_7g081780 | 44.211 | 95 | 46 | 4 | 1 | 92 | 947 | 1037 | 3.37e-18 | 78.2 |
| MS.gene026129.t1 | MTR_7g007630 | 42.708 | 96 | 49 | 2 | 1 | 95 | 666 | 756 | 5.85e-18 | 77.4 |
| MS.gene026129.t1 | MTR_7g407130 | 42.708 | 96 | 49 | 2 | 1 | 95 | 696 | 786 | 7.12e-18 | 77.4 |
| MS.gene026129.t1 | MTR_7g096980 | 34.021 | 97 | 62 | 1 | 1 | 97 | 1108 | 1202 | 2.20e-17 | 75.9 |
| MS.gene026129.t1 | MTR_7g007560 | 43.158 | 95 | 48 | 2 | 1 | 94 | 653 | 742 | 2.85e-17 | 75.5 |
| MS.gene026129.t1 | MTR_7g010000 | 39.394 | 99 | 50 | 3 | 1 | 96 | 746 | 837 | 2.16e-15 | 70.1 |
| MS.gene026129.t1 | MTR_7g007620 | 43.750 | 96 | 48 | 2 | 1 | 95 | 620 | 710 | 2.58e-15 | 70.1 |
| MS.gene026129.t1 | MTR_7g009970 | 39.796 | 98 | 47 | 3 | 1 | 94 | 669 | 758 | 2.87e-14 | 67.0 |
| MS.gene026129.t1 | MTR_8g047220 | 42.708 | 96 | 49 | 2 | 1 | 95 | 831 | 921 | 2.90e-14 | 67.0 |
| MS.gene026129.t1 | MTR_0491s0030 | 41.667 | 96 | 50 | 2 | 1 | 95 | 704 | 794 | 3.11e-14 | 67.0 |
| MS.gene026129.t1 | MTR_8g047210 | 42.708 | 96 | 49 | 2 | 1 | 95 | 768 | 858 | 3.47e-14 | 66.6 |
| MS.gene026129.t1 | MTR_8g047230 | 41.667 | 96 | 50 | 2 | 1 | 95 | 875 | 965 | 3.81e-14 | 66.6 |
| MS.gene026129.t1 | MTR_7g013680 | 36.842 | 95 | 54 | 3 | 1 | 94 | 619 | 708 | 5.54e-14 | 66.2 |
| MS.gene026129.t1 | MTR_7g013350 | 40.000 | 95 | 51 | 3 | 1 | 94 | 295 | 384 | 3.41e-13 | 63.9 |
| MS.gene026129.t1 | MTR_8g047160 | 41.573 | 89 | 46 | 2 | 1 | 88 | 531 | 614 | 6.44e-13 | 63.2 |
| MS.gene026129.t1 | MTR_7g009940 | 36.082 | 97 | 50 | 2 | 3 | 95 | 700 | 788 | 7.28e-13 | 63.2 |
| MS.gene026129.t1 | MTR_7g407090 | 37.895 | 95 | 53 | 2 | 1 | 94 | 651 | 740 | 7.55e-13 | 62.8 |
| MS.gene026129.t1 | MTR_2g070020 | 34.021 | 97 | 52 | 2 | 1 | 90 | 987 | 1078 | 5.19e-12 | 60.5 |
| MS.gene026129.t1 | MTR_1g079520 | 32.432 | 111 | 51 | 3 | 1 | 97 | 946 | 1046 | 8.60e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026129.t1 | AT4G08850 | 40.426 | 94 | 52 | 1 | 1 | 94 | 955 | 1044 | 2.28e-18 | 78.6 |
| MS.gene026129.t1 | AT1G35710 | 41.489 | 94 | 50 | 2 | 1 | 94 | 1031 | 1119 | 1.16e-17 | 76.6 |
| MS.gene026129.t1 | AT1G17230 | 37.255 | 102 | 42 | 4 | 1 | 90 | 977 | 1068 | 1.08e-12 | 62.8 |
| MS.gene026129.t1 | AT1G17230 | 37.255 | 102 | 42 | 4 | 1 | 90 | 977 | 1068 | 1.09e-12 | 62.8 |
| MS.gene026129.t1 | AT2G33170 | 35.238 | 105 | 55 | 4 | 1 | 97 | 1003 | 1102 | 1.36e-11 | 59.3 |
| MS.gene026129.t1 | AT2G33170 | 35.238 | 105 | 55 | 4 | 1 | 97 | 1003 | 1102 | 1.36e-11 | 59.3 |
| MS.gene026129.t1 | AT2G01210 | 34.831 | 89 | 56 | 1 | 4 | 90 | 616 | 704 | 9.59e-11 | 57.0 |
Find 23 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCGCATTCTCGCCCTACTA+TGG | 0.314752 | 1.2:+25000548 | MS.gene026129:CDS |
| AAATGTGATGTGCTTAGTTT+TGG | 0.323764 | 1.2:+25000324 | MS.gene026129:CDS |
| CTCTTGAACAATGGTATTTG+TGG | 0.410722 | 1.2:-25000481 | None:intergenic |
| CTGCAGACGTGCTCCATAGT+AGG | 0.433015 | 1.2:-25000561 | None:intergenic |
| GAGATATTGTAACTTATTTG+TGG | 0.436360 | 1.2:+25000385 | MS.gene026129:CDS |
| AATGCCATTGATAGATAAGT+TGG | 0.448507 | 1.2:+25000449 | MS.gene026129:CDS |
| CTACTATGGAGCACGTCTGC+AGG | 0.462298 | 1.2:+25000562 | MS.gene026129:CDS |
| CCATAGTAGGGCGAGAATGC+GGG | 0.475941 | 1.2:-25000548 | None:intergenic |
| AAGAGGTGGCATCGATGATA+AGG | 0.477261 | 1.2:+25000499 | MS.gene026129:CDS |
| ATGGCATTGTATCAAGTCTC+AGG | 0.500257 | 1.2:-25000434 | None:intergenic |
| AAATAAGTTACAATATCTCC+AGG | 0.507529 | 1.2:-25000381 | None:intergenic |
| TGCAGACGTGCTCCATAGTA+GGG | 0.523643 | 1.2:-25000560 | None:intergenic |
| GGGTCCAACTTATCTATCAA+TGG | 0.537807 | 1.2:-25000453 | None:intergenic |
| CACAAATACCATTGTTCAAG+AGG | 0.544989 | 1.2:+25000482 | MS.gene026129:CDS |
| ACAACCTTCACAAAGTGTTA+CGG | 0.557625 | 1.2:+25000410 | MS.gene026129:CDS |
| ACAATGGTATTTGTGGGATG+AGG | 0.558404 | 1.2:-25000474 | None:intergenic |
| TCCATAGTAGGGCGAGAATG+CGG | 0.563103 | 1.2:-25000549 | None:intergenic |
| CGATGCCACCTCTTGAACAA+TGG | 0.568011 | 1.2:-25000490 | None:intergenic |
| TCTTGAACAATGGTATTTGT+GGG | 0.579159 | 1.2:-25000480 | None:intergenic |
| CAATGGTATTTGTGGGATGA+GGG | 0.597210 | 1.2:-25000473 | None:intergenic |
| AGGTCCGTAACACTTTGTGA+AGG | 0.636371 | 1.2:-25000414 | None:intergenic |
| AAATACCATTGTTCAAGAGG+TGG | 0.646197 | 1.2:+25000485 | MS.gene026129:CDS |
| AATAAGTTACAATATCTCCA+GGG | 0.667842 | 1.2:-25000380 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATAAGTTACAATATCTCC+AGG | - | chr1.2:25000384-25000403 | None:intergenic | 25.0% |
| ! | AATAAGTTACAATATCTCCA+GGG | - | chr1.2:25000383-25000402 | None:intergenic | 25.0% |
| ! | GAGATATTGTAACTTATTTG+TGG | + | chr1.2:25000385-25000404 | MS.gene026129:CDS | 25.0% |
| !! | TTAATCTTGGAAATGCTTTT+TGG | + | chr1.2:25000351-25000370 | MS.gene026129:CDS | 25.0% |
| !!! | TAGTTTTGGGATATTAATCT+TGG | + | chr1.2:25000338-25000357 | MS.gene026129:CDS | 25.0% |
| AATGCCATTGATAGATAAGT+TGG | + | chr1.2:25000449-25000468 | MS.gene026129:CDS | 30.0% | |
| TCTTGAACAATGGTATTTGT+GGG | - | chr1.2:25000483-25000502 | None:intergenic | 30.0% | |
| ! | AAATGTGATGTGCTTAGTTT+TGG | + | chr1.2:25000324-25000343 | MS.gene026129:CDS | 30.0% |
| !! | AATGTGATGTGCTTAGTTTT+GGG | + | chr1.2:25000325-25000344 | MS.gene026129:CDS | 30.0% |
| AAATACCATTGTTCAAGAGG+TGG | + | chr1.2:25000485-25000504 | MS.gene026129:CDS | 35.0% | |
| CACAAATACCATTGTTCAAG+AGG | + | chr1.2:25000482-25000501 | MS.gene026129:CDS | 35.0% | |
| CTCTTGAACAATGGTATTTG+TGG | - | chr1.2:25000484-25000503 | None:intergenic | 35.0% | |
| ! | ACAACCTTCACAAAGTGTTA+CGG | + | chr1.2:25000410-25000429 | MS.gene026129:CDS | 35.0% |
| ACAATGGTATTTGTGGGATG+AGG | - | chr1.2:25000477-25000496 | None:intergenic | 40.0% | |
| ATGGCATTGTATCAAGTCTC+AGG | - | chr1.2:25000437-25000456 | None:intergenic | 40.0% | |
| CAATGGTATTTGTGGGATGA+GGG | - | chr1.2:25000476-25000495 | None:intergenic | 40.0% | |
| GGGTCCAACTTATCTATCAA+TGG | - | chr1.2:25000456-25000475 | None:intergenic | 40.0% | |
| AGGTCCGTAACACTTTGTGA+AGG | - | chr1.2:25000417-25000436 | None:intergenic | 45.0% | |
| !! | AAGAGGTGGCATCGATGATA+AGG | + | chr1.2:25000499-25000518 | MS.gene026129:CDS | 45.0% |
| !!! | ATGCTTTTTGGAAAGCACCC+TGG | + | chr1.2:25000363-25000382 | MS.gene026129:CDS | 45.0% |
| CGATGCCACCTCTTGAACAA+TGG | - | chr1.2:25000493-25000512 | None:intergenic | 50.0% | |
| TCCATAGTAGGGCGAGAATG+CGG | - | chr1.2:25000552-25000571 | None:intergenic | 50.0% | |
| TGCAGACGTGCTCCATAGTA+GGG | - | chr1.2:25000563-25000582 | None:intergenic | 50.0% | |
| CCATAGTAGGGCGAGAATGC+GGG | - | chr1.2:25000551-25000570 | None:intergenic | 55.0% | |
| CTACTATGGAGCACGTCTGC+AGG | + | chr1.2:25000562-25000581 | MS.gene026129:CDS | 55.0% | |
| CTGCAGACGTGCTCCATAGT+AGG | - | chr1.2:25000564-25000583 | None:intergenic | 55.0% | |
| CCCGCATTCTCGCCCTACTA+TGG | + | chr1.2:25000548-25000567 | MS.gene026129:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 25000309 | 25000602 | 25000309 | ID=MS.gene026129 |
| chr1.2 | mRNA | 25000309 | 25000602 | 25000309 | ID=MS.gene026129.t1;Parent=MS.gene026129 |
| chr1.2 | exon | 25000309 | 25000602 | 25000309 | ID=MS.gene026129.t1.exon1;Parent=MS.gene026129.t1 |
| chr1.2 | CDS | 25000309 | 25000602 | 25000309 | ID=cds.MS.gene026129.t1;Parent=MS.gene026129.t1 |
| Gene Sequence |
| Protein sequence |
