AlfalfaGEDB | MS.gene026324

M. sativa cultivar XinJiangDaYe / MS.gene026324

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene026324.t1	RHN78223.1	73.9	337	61	3	71	396	13	333	9.10E-118	433.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene026324.t1	Q8L3W1	23.8	319	200	9	78	356	21	336	3.7e-11	70.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene026324.t1	A0A396JPN4	73.9	337	61	3	71	396	13	333	6.5e-118	433.7

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene026324.t1	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene026324.t1	MTR_1g035570	70.091	331	71	4	71	394	13	322	4.07e-146	421
MS.gene026324.t1	MTR_1g035640	50.585	342	118	4	71	395	13	320	5.81e-105	318
MS.gene026324.t1	MTR_1g035640	58.182	55	23	0	1	55	243	297	4.04e-12	67.8
MS.gene026324.t1	MTR_1g035620	45.062	324	126	8	78	391	24	305	2.06e-80	253
MS.gene026324.t1	MTR_1g035490	65.753	219	51	5	189	394	20	227	7.75e-77	241
MS.gene026324.t1	MTR_1g050845	44.615	325	114	8	78	391	24	293	2.82e-73	244
MS.gene026324.t1	MTR_6g061070	35.000	300	172	7	79	361	34	327	7.00e-49	172
MS.gene026324.t1	MTR_6g061070	75.510	49	12	0	1	49	285	333	7.24e-18	85.5
MS.gene026324.t1	MTR_1g035650	43.750	208	80	4	78	276	24	203	2.71e-45	155
MS.gene026324.t1	MTR_7g050710	31.922	307	174	10	78	366	28	317	3.30e-34	129

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene026324.t1	AT3G18990	25.076	327	196	9	76	359	19	339	3.67e-18	85.9
MS.gene026324.t1	AT3G18990	25.231	325	194	9	76	357	19	337	7.87e-18	84.3

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Find 67 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATACCAAAGCAAACTCAA+AGG	0.654830	1.1:+20028740	MS.gene026324:CDS
AAATACCTTGAAACTGCTCC+AGG	0.519839	1.1:-20027069	None:intergenic
AAGACATTGGAGGAACATCT+AGG	0.526190	1.1:+20029736	MS.gene026324:CDS
AAGAGATATCCATATTTGAA+AGG	0.520085	1.1:-20028463	None:intergenic
AAGTGATGAATCAAGTGATG+AGG	0.567308	1.1:+20028657	MS.gene026324:CDS
AATATCAAATCAGTTGATGA+AGG	0.540855	1.1:+20028562	MS.gene026324:CDS
AATATTGTATGGGTGGTGGA+TGG	0.587908	1.1:+20029434	MS.gene026324:CDS
AATGGCAAGAGAAAATTCTC+AGG	0.523324	1.1:+20028769	MS.gene026324:CDS
ACTTACAAAGAAATAGCCAT+TGG	0.605595	1.1:-20029225	None:intergenic
ACTTTCTTCACCTTCTTCAA+TGG	0.380996	1.1:-20028683	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAATATTGTATGGGTGG+TGG	+	chr1.1:20029430-20029449	MS.gene026324:CDS	30.0%
AAATACCAAAGCAAACTCAA+AGG	+	chr1.1:20028740-20028759	MS.gene026324:CDS	30.0%
AAGATGATCCTATGAAAATC+TGG	-	chr1.1:20028158-20028177	None:intergenic	30.0%
ACTTACAAAGAAATAGCCAT+TGG	-	chr1.1:20029228-20029247	None:intergenic	30.0%
AGAAAACCTAGTGATTTCTT+TGG	-	chr1.1:20029577-20029596	None:intergenic	30.0%
CAGAAAAAATATTGTATGGG+TGG	+	chr1.1:20029427-20029446	MS.gene026324:CDS	30.0%
CATATACTACACACACAAAT+AGG	+	chr1.1:20027651-20027670	MS.gene026324:intron	30.0%
CCATGACAAGGTTATAAAAA+AGG	+	chr1.1:20029681-20029700	MS.gene026324:intron	30.0%
GAATGAAACATTCAATGACT+CGG	+	chr1.1:20027612-20027631	MS.gene026324:intron	30.0%
TAAACCATTACTTGAAACAG+TGG	+	chr1.1:20029296-20029315	MS.gene026324:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	20026938	20029777	20026938	ID=MS.gene026324
chr1.1	mRNA	20026938	20029777	20026938	ID=MS.gene026324.t1;Parent=MS.gene026324
chr1.1	exon	20026938	20027085	20026938	ID=MS.gene026324.t1.exon1;Parent=MS.gene026324.t1
chr1.1	CDS	20026938	20027085	20026938	ID=cds.MS.gene026324.t1;Parent=MS.gene026324.t1
chr1.1	exon	20027312	20027366	20027312	ID=MS.gene026324.t1.exon2;Parent=MS.gene026324.t1
chr1.1	CDS	20027312	20027366	20027312	ID=cds.MS.gene026324.t1;Parent=MS.gene026324.t1
chr1.1	exon	20027822	20027849	20027822	ID=MS.gene026324.t1.exon3;Parent=MS.gene026324.t1
chr1.1	CDS	20027822	20027849	20027822	ID=cds.MS.gene026324.t1;Parent=MS.gene026324.t1
chr1.1	exon	20028313	20028790	20028313	ID=MS.gene026324.t1.exon4;Parent=MS.gene026324.t1
chr1.1	CDS	20028313	20028790	20028313	ID=cds.MS.gene026324.t1;Parent=MS.gene026324.t1
chr1.1	exon	20029123	20029241	20029123	ID=MS.gene026324.t1.exon5;Parent=MS.gene026324.t1
chr1.1	CDS	20029123	20029241	20029123	ID=cds.MS.gene026324.t1;Parent=MS.gene026324.t1
chr1.1	exon	20029316	20029589	20029316	ID=MS.gene026324.t1.exon6;Parent=MS.gene026324.t1
chr1.1	CDS	20029316	20029589	20029316	ID=cds.MS.gene026324.t1;Parent=MS.gene026324.t1
chr1.1	exon	20029691	20029777	20029691	ID=MS.gene026324.t1.exon7;Parent=MS.gene026324.t1
chr1.1	CDS	20029691	20029777	20029691	ID=cds.MS.gene026324.t1;Parent=MS.gene026324.t1

Gene Sequence

>MS.gene026324

ATGCGAGCTGGTTGGAAGTCGTTTGTGCAGGAACATAACCTGCAAGCTGGTGATGTATGTAAATTTGTGATGACTCAACGAAAACCACTTTCATTCACAATTACTATTATCCCAGCTACAAAGGAACCATGCCTGGAGCAGTTTCAAGGTATTTCTTTCGTTATTATTGTTTTTGGAAAGATACATATTTTATTGTAGTTTAATCTTGATACATTTGATCAATGTAAAAAGTTTTTTAATTTGTCAACAAATTACAATCAATCATATATGTTCATCATGGAATAGTCACCATAATATATAAGTATTTGGTTTTAGTCATTCTAATATTGGCTACATAAAGACTAAAATAAATATTATGCATGTATATATTACAGGGATTAATTCTAACATACACAAATTTTACAAGCACAAACAGAACAATGGTCAAAAGTAAAAAATGATATGTTCACTATGATTGAAGTTTTTTTCCAAGTACTAAAGGTGATATATAACAAATTTAAACTAATTTTTAATGATCTAAGATCATTGATTTATACTGTAAATATCTTTACACATTCATATGAGTTAATTTAATCAATTTTGTTAGTTGGTTTTTACCTAAAAAGTGATTTGAGCCAAAACTATGTCATCATAAAGTAACTAAAGCTATCTAATTAATTAGACATTAGAAAACAGAATGAAACATTCAATGACTCGGTCATGTTAAAGTATGACATATACTACACACACAAATAGGACAAAGCAAATCCACTTTTGTGGCAAATTAAAAGTAGCTTCTATTTATAGCTTACATTGTGAGCTCATGTGAAATGGACCCTACAGTACCTTATGTAAAAATGGCAGATATTAAGGGATCAAACCATTGTGTTTTTTAATGTATGCAGTGTGAGACTGTTGGATCATCTCAAGCATGTATAGCTTTCACTGAATTGTACTAACTGCTAAACACTTTTTTATCGTGTCATAAATGGCAAGTTCAATCCAACACCAAAGTAGTAACACCAGAAAAAATTAGATAGAAAATTCATCATTTCTCTACATGACCTGTACTGGACAGTAATAAAGTCTTGGTTTTCTACCATACTACAAAAAATATCTTTAACTTCATAACATTCACACTTCATAATATTCAGACCTAACACTTAAAATCTTTCACCTTACTTCTCTAAGCCTTAGCTCAATACTCACTCACTAACATCATGTCATTTCAATATATTCCAGATTTTCATAGGATCATCTTGCAAGATAAGAAACTTGTAAGCATATTAGAAAGAGTTATCTTGCATATTTACATTCTGTTTCATTTATAATATATTATTTATGTATCATTTTTTAAAAAATGTTTATGTATGATTTTTGAATTTCTTTGTTGCAGAGGGTTCCAAAGAAATATGTGGAGAAATATTGGAAAGGAATATCAAACCCTATATTCCTTAAGTTTCCTAACGGTGTTGAACAAAAAATATTTTGGGTGGAGAGTAATGGTGATATTTGGTTTCAAAAAAATTGGGAAAATTTTGCAAAGCCTTTCAAATATGGATATCTCTTAACTTTCAAATTCATTGGTGGATCATATTTTAAGGTTAAGATATTTGGTGCCAATACTTTAGAAATAAACTATTCCAATATCAAATCAGTTGATGAAGGTGCTGAAGACACAGAAGAAGGTAAAGAAAGAGATGAATCAAGTGATGATGAAGTCACAAAAGAAGCTCAAGAAAGTGATGAATCAAGTGATGAGGAAGCCATTGAAGAAGGTGAAGAAAGTGATGAAGAGAGTAATGAGAGTTCAGATGAGGTTGAAATACCAAAGCAAACTCAAAGGACCAGAAATGGCAAGAGAAAATTCTCAGGTTGATCTTTTTTGGGTAGATTTAGGCTTGTCTGTTACTCTATTTCTTTAAAAATAAATCACTTGTTTTTATAGAAATAATTATTTTAAGAGTTATTGAATGGTTTGTTGGAATTTTATGTCTGAAAGGTTGAAAATCTTTGAAAGAAAAATGTCTTCAAGTGCTGAGTAGATACTTTTTATATAAAAATGTGATTTTTTTTTTGTAGGAAAAATATGTGATTTTTATTATGATAAAAAAAATAATAAATACTTAATTGGTTTTCTTAAAATCTTCTAAAAACAATATCTAAATCAAATGTATAATAAATAGTTAATGCCTAAACTTGGCAGGTAGTAACAAAGTGGCCATGGTAAAGAAGGCTAGAAAATGCCTAAGATCTGAAGCTGTTAATGAGAATCCCTCTTTTGAAGTTATAATGTCACAATCTTACGCCAATGGCTATTTCTTTGTAAGTTTGCTATACAAAGTATCTATTAATTTTAGCTTTAATTATTATATCTAATAAACCATTACTTGAAACAGTGGATACCAAATGAGTTTTCAAGAGAACACTTGAAAGATTTTAATGGAACCACAACAATAAGGGTTGGTAATGAAAGGAGTGTGGAAGTGAGTGTGAGGTATTATGATACCCAGAAAAAATATTGTATGGGTGGTGGATGGAAAATATTCAGGCAAAAATATAATTTGCAAGTTGATGATGTTTGTAAATTTGAGATGATTCAACGGAGACCAATTTCATTCAATGTTACTATTACTCGTGCCAAAAACGAACCAAAGCCAAAGAAATCACTAGGTTTTCTTTCTTTCTTTTTTATTTGGTTTTCTGTATGTTATAATTTTTTTTATTTGATATTTGATTAACATCTAATATTTTTTTTTGTTATCCATGACAAGGTTATAAAAAAGGGGTCTCTTGTGGTAAGAGTGTTGTAAAGAAGCAAGACATTGGAGGAACATCTAGGAGCCATCCAAAAGTAAAGG

Protein sequence

>MS.gene026324.t1

MRAGWKSFVQEHNLQAGDVCKFVMTQRKPLSFTITIIPATKEPCLEQFQGINSNIHKFYKHKQNNGQNVRLLDHLKHRVPKKYVEKYWKGISNPIFLKFPNGVEQKIFWVESNGDIWFQKNWENFAKPFKYGYLLTFKFIGGSYFKVKIFGANTLEINYSNIKSVDEGAEDTEEGKERDESSDDEVTKEAQESDESSDEEAIEEGEESDEESNESSDEVEIPKQTQRTRNGKRKFSGSNKVAMVKKARKCLRSEAVNENPSFEVIMSQSYANGYFFWIPNEFSREHLKDFNGTTTIRVGNERSVEVSVRYYDTQKKYCMGGGWKIFRQKYNLQVDDVCKFEMIQRRPISFNVTITRAKNEPKPKKSLGYKKGVSCGKSVVKKQDIGGTSRSHPKVK