AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene026335


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026335.t1 MTR_4g119990 66.159 328 97 7 1 319 1 323 1.40e-144 412
MS.gene026335.t1 MTR_6g043890 65.902 305 90 6 1 299 1 297 9.11e-134 384
MS.gene026335.t1 MTR_1g035440 59.671 243 72 4 1 217 1 243 2.14e-76 234
MS.gene026335.t1 MTR_3g098790 30.293 307 191 8 9 307 7 298 4.40e-33 125
MS.gene026335.t1 MTR_5g044670 30.792 341 193 14 1 322 1 317 1.12e-32 124
MS.gene026335.t1 MTR_7g050720 29.206 315 195 9 13 307 8 314 2.03e-31 120
MS.gene026335.t1 MTR_1g108780 27.241 290 195 7 31 312 13 294 1.39e-30 120
MS.gene026335.t1 MTR_1g035460 29.605 304 186 9 31 316 2 295 2.97e-30 118
MS.gene026335.t1 MTR_3g061370 28.387 310 180 10 9 307 3 281 1.15e-29 114
MS.gene026335.t1 MTR_7g050710 29.167 312 161 9 13 293 11 293 1.31e-29 115
MS.gene026335.t1 MTR_7g050560 29.268 287 176 7 13 293 11 276 2.23e-29 114
MS.gene026335.t1 MTR_8g103940 26.367 311 214 8 13 316 37 339 5.14e-29 116
MS.gene026335.t1 MTR_3g061350 29.085 306 175 11 13 307 10 284 1.20e-28 112
MS.gene026335.t1 MTR_3g061320 28.197 305 174 10 13 307 10 279 3.17e-28 110
MS.gene026335.t1 MTR_1g035570 29.730 296 175 8 24 301 14 294 6.96e-28 112
MS.gene026335.t1 MTR_6g061070 27.327 333 209 12 9 319 12 333 1.04e-27 112
MS.gene026335.t1 MTR_3g061310 29.276 304 178 10 13 307 10 285 2.92e-27 108
MS.gene026335.t1 MTR_1g035640 30.547 311 178 13 24 322 14 298 3.60e-27 111
MS.gene026335.t1 MTR_3g061340 29.153 295 162 12 15 300 12 268 1.58e-26 106
MS.gene026335.t1 MTR_7g050580 29.514 288 174 7 13 293 11 276 2.91e-26 106
MS.gene026335.t1 MTR_3g061330 27.734 256 150 8 13 262 10 236 6.69e-24 98.6
MS.gene026335.t1 MTR_1g033740 32.178 202 102 8 1 180 1 189 3.54e-22 92.4
MS.gene026335.t1 MTR_1g035620 28.328 293 164 9 15 295 9 267 8.27e-22 95.5
MS.gene026335.t1 MTR_7g050590 28.723 282 165 10 31 293 2 266 2.26e-21 92.4
MS.gene026335.t1 MTR_1g035480 33.702 181 94 7 1 167 1 169 3.69e-21 89.4
MS.gene026335.t1 MTR_3g061290 38.000 150 81 7 13 153 10 156 1.10e-20 88.6
MS.gene026335.t1 MTR_1g050845 27.875 287 161 8 15 295 9 255 2.93e-20 92.0
MS.gene026335.t1 MTR_1g035510 30.088 226 121 9 31 242 2 204 6.67e-20 87.0
MS.gene026335.t1 MTR_1g035500 33.136 169 90 6 9 165 19 176 1.77e-19 85.1
MS.gene026335.t1 MTR_3g005490 27.063 303 148 11 14 307 20 258 5.93e-19 85.1
MS.gene026335.t1 MTR_1g021380 27.653 311 183 12 13 293 20 318 1.05e-16 80.1
MS.gene026335.t1 MTR_3g005480 35.965 114 66 2 14 124 20 129 1.30e-16 78.6
MS.gene026335.t1 MTR_5g070440 27.703 296 164 11 14 307 20 267 1.99e-16 78.2
MS.gene026335.t1 MTR_1g035470 40.625 96 53 3 31 126 2 93 7.93e-16 73.6
MS.gene026335.t1 MTR_3g005460 25.352 284 169 9 13 293 260 503 3.12e-15 76.6
MS.gene026335.t1 MTR_3g005460 23.642 313 148 10 14 314 24 257 1.57e-13 71.6
MS.gene026335.t1 MTR_1g035650 30.464 151 98 3 15 165 9 152 2.39e-14 71.2
MS.gene026335.t1 MTR_3g005495 30.636 173 106 4 14 175 242 411 3.45e-14 73.2
MS.gene026335.t1 MTR_3g005495 24.316 329 203 13 31 320 29 350 1.32e-11 65.5
MS.gene026335.t1 MTR_1g021360 26.780 295 158 12 26 293 193 456 4.54e-14 73.2
MS.gene026335.t1 MTR_1g021400 24.159 327 186 11 13 293 28 338 9.22e-13 68.6
MS.gene026335.t1 MTR_3g005420 24.415 299 170 10 14 307 288 535 1.27e-12 68.6
MS.gene026335.t1 MTR_3g061220 30.909 110 60 2 13 122 10 103 2.46e-11 60.5
MS.gene026335.t1 MTR_4g068320 35.135 111 69 2 15 123 6 115 5.76e-11 63.5
MS.gene026335.t1 MTR_1g021410 24.367 316 194 13 16 293 23 331 7.88e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026335.t1 AT4G33280 24.620 329 197 8 13 315 23 326 3.42e-16 78.6
MS.gene026335.t1 AT3G18960 33.333 111 73 1 5 115 20 129 2.66e-14 71.2
MS.gene026335.t1 AT3G18960 33.333 111 73 1 5 115 20 129 3.03e-14 70.9
MS.gene026335.t1 AT1G49475 36.036 111 69 2 15 125 34 142 6.44e-14 69.7
MS.gene026335.t1 AT4G01580 31.532 111 75 1 5 115 20 129 3.56e-13 67.4

Find 0 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATAAAAAATCTCATTA+TGG - 10221:22518-22537 MS.gene026335:intergenic 10.0%
!!! AACAATTTATGAAAATTTTA+TGG + 10221:23084-23103 MS.gene026335:intron 10.0%
!!! TATTTTTTGTCTTATTTTAT+AGG + 10221:22128-22147 MS.gene026335:intron 10.0%
!! GGAAATTCATTTAATATAAT+GGG + 10221:23048-23067 MS.gene026335:intron 15.0%
!! TATAATTTACCTTCAAAATA+AGG - 10221:23011-23030 MS.gene026335:intergenic 15.0%
!!! TGAAATTATTAATCTAAAAC+AGG + 10221:22728-22747 MS.gene026335:intron 15.0%
!! AAAATGTGATCTTATGATAT+TGG + 10221:21410-21429 MS.gene026335:intron 20.0%
!! AATAGTGATTGTTCAAATAT+AGG + 10221:21454-21473 MS.gene026335:intron 20.0%
!! AATCTCATTATGGTATAATT+TGG - 10221:22508-22527 MS.gene026335:intergenic 20.0%
!! AGAAAAACAATTTCTTCATT+AGG - 10221:21362-21381 MS.gene026335:intergenic 20.0%
!! ATTTATCTTTGTCAACAAAA+AGG - 10221:22815-22834 MS.gene026335:intergenic 20.0%
!! ATTTCAAATTATTCTCTTCA+CGG - 10221:22896-22915 MS.gene026335:intergenic 20.0%
!! CAAATATGCATTACAAAATT+TGG + 10221:21258-21277 MS.gene026335:intron 20.0%
!! CAATATAATAATTCATGTGT+TGG + 10221:23129-23148 MS.gene026335:intron 20.0%
!! CGGAAATTCATTTAATATAA+TGG + 10221:23047-23066 MS.gene026335:intron 20.0%
!! GAAAAACAATTTCTTCATTA+GGG - 10221:21361-21380 MS.gene026335:intergenic 20.0%
!! TATATTAAATGAATTTCCGA+TGG - 10221:23046-23065 MS.gene026335:intergenic 20.0%
!! TTCAAGATAAAGTAGTTAAA+TGG - 10221:22712-22731 MS.gene026335:intergenic 20.0%
!! TTTATCTTTGTCAACAAAAA+GGG - 10221:22814-22833 MS.gene026335:intergenic 20.0%
!!! GAAGAGAATAATTTGAAATT+TGG + 10221:22897-22916 MS.gene026335:CDS 20.0%
!!! TAAAATGTATGTGTATTGAT+AGG + 10221:22674-22693 MS.gene026335:intron 20.0%
!!! TGATCACTTTTATTTTTGAA+AGG + 10221:22343-22362 MS.gene026335:intron 20.0%
!!! TGTTGCTACTAATTTTTTTT+TGG + 10221:22151-22170 MS.gene026335:intron 20.0%
!!! TTTGAATGAATCACAATTTT+TGG + 10221:21780-21799 MS.gene026335:CDS 20.0%
!!! TTTTTGTTGACAAAGATAAA+TGG + 10221:22814-22833 MS.gene026335:CDS 20.0%
! AAGTAGCTGTAATGATATTA+AGG + 10221:22198-22217 MS.gene026335:CDS 25.0%
! CAAAAAATTTCTTCACTTTC+TGG - 10221:22243-22262 MS.gene026335:intergenic 25.0%
! CTATTCTTTGATGAGATTAA+TGG + 10221:22449-22468 MS.gene026335:intron 25.0%
! CTCTTTAATGTGATTGTATT+CGG + 10221:21826-21845 MS.gene026335:CDS 25.0%
! GAAGAATAACCTTAAAAAAG+TGG - 10221:21169-21188 MS.gene026335:intergenic 25.0%
! GAGAAAATAACAACTTGAAA+TGG - 10221:22966-22985 MS.gene026335:intergenic 25.0%
! TATATTTGATTAGTGTCCAT+CGG + 10221:23027-23046 MS.gene026335:intron 25.0%
! TGAATTTGTCAAGAAACATA+TGG + 10221:22761-22780 MS.gene026335:CDS 25.0%
! TGCATATTGTGAAAAATCAA+GGG - 10221:21240-21259 MS.gene026335:intergenic 25.0%
! TGTTTCTTGACAAATTCATT+TGG - 10221:22759-22778 MS.gene026335:intergenic 25.0%
! TTGCATATTGTGAAAAATCA+AGG - 10221:21241-21260 MS.gene026335:intergenic 25.0%
!! ATACCTTTTCACAAAACTTA+TGG - 10221:21629-21648 MS.gene026335:intergenic 25.0%
!! TACCAATTTTTCTTCTTGAA+GGG - 10221:21198-21217 MS.gene026335:intergenic 25.0%
!! TACCTTTTCACAAAACTTAT+GGG - 10221:21628-21647 MS.gene026335:intergenic 25.0%
!! TATGATGTTACATTTTGCAA+TGG + 10221:21730-21749 MS.gene026335:CDS 25.0%
!! TTACCAATTTTTCTTCTTGA+AGG - 10221:21199-21218 MS.gene026335:intergenic 25.0%
!!! GAATTCTCAATTTTTTTAGC+AGG + 10221:22175-22194 MS.gene026335:intron 25.0%
!!! TAAGTTTTGTGAAAAGGTAT+TGG + 10221:21629-21648 MS.gene026335:CDS 25.0%
!!! TCAAGTTGTTATTTTCTCTT+TGG + 10221:22968-22987 MS.gene026335:CDS 25.0%
!!! TGTTATTTTCTCTTTGGAAA+AGG + 10221:22974-22993 MS.gene026335:CDS 25.0%
!!! TTGAAAACTCCTTATTTTGA+AGG + 10221:22999-23018 MS.gene026335:CDS 25.0%
AGTGATTGTTCAAATATAGG+TGG + 10221:21457-21476 MS.gene026335:intron 30.0%
ATACATTGATAGAGATCTCT+TGG + 10221:22303-22322 MS.gene026335:CDS 30.0%
ATCAACTCGAATGAACAAAA+AGG + 10221:22006-22025 MS.gene026335:CDS 30.0%
CCAATCACAATCTATTATGT+AGG - 10221:22564-22583 MS.gene026335:intergenic 30.0%
CCTACATAATAGATTGTGAT+TGG + 10221:22561-22580 MS.gene026335:intron 30.0%
TACCCTTCAAGAAGAAAAAT+TGG + 10221:21193-21212 MS.gene026335:CDS 30.0%
TTCTTACACACTTTCATAGA+CGG - 10221:21979-21998 MS.gene026335:intergenic 30.0%
! ATGTTACATTTTGCAATGGT+TGG + 10221:21734-21753 MS.gene026335:CDS 30.0%
!! TGTGTTTTTGTGCTAAACAA+AGG + 10221:22927-22946 MS.gene026335:CDS 30.0%
!! TTTGTGAAAAGGTATTGGAA+AGG + 10221:21634-21653 MS.gene026335:CDS 30.0%
!!! GGTGGAAAAGAAAGTTTTTT+GGG + 10221:21693-21712 MS.gene026335:CDS 30.0%
!!! TAATGGTGTCCACTTTTTTA+AGG + 10221:21157-21176 MS.gene026335:CDS 30.0%
!!! TGGTGGAAAAGAAAGTTTTT+TGG + 10221:21692-21711 MS.gene026335:CDS 30.0%
AAACAATCTGCACTAACTTG+TGG + 10221:22858-22877 MS.gene026335:CDS 35.0%
CAAGAAACATATGGACATGA+TGG + 10221:22770-22789 MS.gene026335:CDS 35.0%
GAACATGATCATCAGAGTAA+TGG + 10221:21140-21159 MS.gene026335:CDS 35.0%
GTGATTGTATTCGGTAAATG+CGG + 10221:21835-21854 MS.gene026335:CDS 35.0%
TAACAAACCCAGTTACTCTA+AGG + 10221:21659-21678 MS.gene026335:CDS 35.0%
TTGGAAACCTTAGAGTAACT+GGG - 10221:21669-21688 MS.gene026335:intergenic 35.0%
TTTGGAAACCTTAGAGTAAC+TGG - 10221:21670-21689 MS.gene026335:intergenic 35.0%
! AAACATATGGACATGATGGA+AGG + 10221:22774-22793 MS.gene026335:CDS 35.0%
! ACTTTCTTTTCCACCAAGTT+TGG - 10221:21688-21707 MS.gene026335:intergenic 35.0%
! CTTCACTAGTTTCTCTTGAA+GGG - 10221:21876-21895 MS.gene026335:intergenic 35.0%
! TCTTCACTAGTTTCTCTTGA+AGG - 10221:21877-21896 MS.gene026335:intergenic 35.0%
!! GTTTTTGAGCATGTGAACAA+TGG + 10221:22066-22085 MS.gene026335:CDS 35.0%
!! TACTCTAAGGTTTCCAAACT+TGG + 10221:21672-21691 MS.gene026335:CDS 35.0%
!! TGCCCATAAGTTTTGTGAAA+AGG + 10221:21623-21642 MS.gene026335:CDS 35.0%
AAGGATCTAAGCATGAGAAG+AGG + 10221:22025-22044 MS.gene026335:CDS 40.0%
TTGAGCATGTGAACAATGGT+TGG + 10221:22070-22089 MS.gene026335:intron 40.0%
! AATCTGCACTAACTTGTGGT+TGG + 10221:22862-22881 MS.gene026335:CDS 40.0%
! TCATGTGTTGGTCTATTGCA+GGG + 10221:23141-23160 MS.gene026335:intron 40.0%
! TCTAAGGTTTCCAAACTTGG+TGG + 10221:21675-21694 MS.gene026335:CDS 40.0%
! TTCATGTGTTGGTCTATTGC+AGG + 10221:23140-23159 MS.gene026335:intron 40.0%
ATTAGGGCTGCATCCAACAA+AGG - 10221:21345-21364 MS.gene026335:intergenic 45.0%
CTTCATCATCACGCGGTAAA+AGG + 10221:21938-21957 MS.gene026335:CDS 45.0%
GAGAATCCTTCATCATCACG+CGG + 10221:21931-21950 MS.gene026335:CDS 45.0%
! CATCCAACAAAGGCTAGCTA+GGG - 10221:21335-21354 MS.gene026335:intergenic 45.0%
!! CTTTTACCGCGTGATGATGA+AGG - 10221:21940-21959 MS.gene026335:intergenic 45.0%
CACACTTTCATAGACGGTGC+TGG - 10221:21973-21992 MS.gene026335:intergenic 50.0%
! GCATCCAACAAAGGCTAGCT+AGG - 10221:21336-21355 MS.gene026335:intergenic 50.0%
! GTGTTGGTCTATTGCAGGGT+TGG + 10221:23145-23164 MS.gene026335:intron 50.0%
!! CATCCCTAGCTAGCCTTTGT+TGG + 10221:21329-21348 MS.gene026335:intron 50.0%


Chromosome Type Strat End Strand Name
10221 gene 21128 23172 21128 ID=MS.gene026335
10221 mRNA 21128 23172 21128 ID=MS.gene026335.t1;Parent=MS.gene026335
10221 exon 21128 21214 21128 ID=MS.gene026335.t1.exon1;Parent=MS.gene026335.t1
10221 CDS 21128 21214 21128 ID=cds.MS.gene026335.t1;Parent=MS.gene026335.t1
10221 exon 21619 22087 21619 ID=MS.gene026335.t1.exon2;Parent=MS.gene026335.t1
10221 CDS 21619 22087 21619 ID=cds.MS.gene026335.t1;Parent=MS.gene026335.t1
10221 exon 22194 22324 22194 ID=MS.gene026335.t1.exon3;Parent=MS.gene026335.t1
10221 CDS 22194 22324 22194 ID=cds.MS.gene026335.t1;Parent=MS.gene026335.t1
10221 exon 22750 23020 22750 ID=MS.gene026335.t1.exon4;Parent=MS.gene026335.t1
10221 CDS 22750 23020 22750 ID=cds.MS.gene026335.t1;Parent=MS.gene026335.t1
10221 exon 23162 23172 23162 ID=MS.gene026335.t1.exon5;Parent=MS.gene026335.t1
10221 CDS 23162 23172 23162 ID=cds.MS.gene026335.t1;Parent=MS.gene026335.t1
Gene Sequence

>MS.gene026335

ATGTCTTCTCAAGAACATGATCATCAGAGTAATGGTGTCCACTTTTTTAAGGTTATTCTTCAAAGTACCCTTCAAGAAGAAAAATTGGTAACAATTTTTGTTCTTTCTTCCCTTGATTTTTCACAATATGCAAATATGCATTACAAAATTTGGATTATGAAGTTATCAGTGAAAAATAGTTTACGAACTCTATACATTAATCATCCCTAGCTAGCCTTTGTTGGATGCAGCCCTAATGAAGAAATTGTTTTTCTTTATAATAATTGATATTATTAGTATTGAAAAATGTGATCTTATGATATTGGAAATTAAAACTTTTGATCTGAAATAGTGATTGTTCAAATATAGGTGGATGAATATAGTTTTTTATATAAATTTTGCTAGTTCTCACTTTCATATAACAACAATGCTTGTTTTAATCATAATAGAAATAGTTCGATCTAAAACATTGTATTAGTATTGATTAATGTTGTGTGTTTAATTTGTTACAGAGTGTGCCCATAAGTTTTGTGAAAAGGTATTGGAAAGGAATAACAAACCCAGTTACTCTAAGGTTTCCAAACTTGGTGGAAAAGAAAGTTTTTTGGGAGAAAACTAGTGATTATGATGTTACATTTTGCAATGGTTGGAAAGAACTTGCAAATTATTTGTCTTTGAATGAATCACAATTTTTGGTGTTTCAATATCAAGAAAAGTCTCTCTTTAATGTGATTGTATTCGGTAAATGCGGATTAGAAATAAAATACCCTTCAAGAGAAACTAGTGAAGAATCTGAAGAGAGTGATTGTTCTTTGAAAATTATTGAGAATCCTTCATCATCACGCGGTAAAAGGCTAAAATCTCCAGCACCGTCTATGAAAGTGTGTAAGAAGATGAAAATCAACTCGAATGAACAAAAAGGATCTAAGCATGAGAAGAGGATAGTCGCTAGATTTCATGTTTTTGAGCATGTGAACAATGGTTGGTTTTGTTCTATCATCATGTTATGTTTCTTTTTATCTATTTTTTGTCTTATTTTATAGGTGTTGCTACTAATTTTTTTTTGGTGAATTCTCAATTTTTTTAGCAGGAAGTAGCTGTAATGATATTAAGGAGAGAAGTAGAATTTTGTGCCAGAAAGTGAAGAAATTTTTTGTCTCTGATAACGACTTTTTTGCATGTATGATGCTAAAGACATACATTGATAGAGATCTCTTGGTAATAAACTATTTTATTTGATCACTTTTATTTTTGAAAGGTTATTTGATCACTTTAATTAGTAATAATTCATTAATATATGTGAACATTATCAAAGCAGTGTAGATAAATTCTCATCAATTATCTATTCTTTGATGAGATTAATGGTGTTTATGTAAATATTTTTTACATATCATTCAACCAAATTATACCATAATGAGATTTTTTATATTTTTTCTACGAACACTATAAAATTACCTACATAATAGATTGTGATTGGTTGCATAAGTAAAAAATATAAACAATGCATACTAATTAAATCACTCATTGATATGTCTTATGTATATTTGTGTATTTTTTTTTTTTTGAATAAAATGTATGTGTATTGATAGGTTCAAATTGCATCCATTTAACTACTTTATCTTGAAATTATTAATCTAAAACAGGTCATACCAAATGAATTTGTCAAGAAACATATGGACATGATGGAAGGAAGAGATGATGCTACCCTTTTTGTTGACAAAGATAAATGGAATGTGAAGCTCACTTTAAATAAACAATCTGCACTAACTTGTGGTTGGAGAGAATTCCGTGAAGAGAATAATTTGAAATTTGGTGATGTTTGTGTTTTTGTGCTAAACAAAGGCAAAGAAACAGTTCCATTTCAAGTTGTTATTTTCTCTTTGGAAAAGGACTTGAAAACTCCTTATTTTGAAGGTAAATTATATTTGATTAGTGTCCATCGGAAATTCATTTAATATAATGGGAAAATTGTGATCTAACAATTTATGAAAATTTTATGGTTTGTAAAATTATTTTTTTCATCAATATAATAATTCATGTGTTGGTCTATTGCAGGGTTGGCTTGA

Protein sequence

>MS.gene026335.t1

MSSQEHDHQSNGVHFFKVILQSTLQEEKLSVPISFVKRYWKGITNPVTLRFPNLVEKKVFWEKTSDYDVTFCNGWKELANYLSLNESQFLVFQYQEKSLFNVIVFGKCGLEIKYPSRETSEESEESDCSLKIIENPSSSRGKRLKSPAPSMKVCKKMKINSNEQKGSKHEKRIVARFHVFEHVNNAGSSCNDIKERSRILCQKVKKFFVSDNDFFACMMLKTYIDRDLLVIPNEFVKKHMDMMEGRDDATLFVDKDKWNVKLTLNKQSALTCGWREFREENNLKFGDVCVFVLNKGKETVPFQVVIFSLEKDLKTPYFEGLA