AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene026348

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026348.t1 MTR_1g035420 96.828 662 18 2 1 662 1 659 0.0 1325
MS.gene026348.t1 MTR_1g035400 81.939 526 86 3 146 662 53 578 0.0 870
MS.gene026348.t1 MTR_8g070070 73.673 471 120 3 194 661 299 768 0.0 747
MS.gene026348.t1 MTR_8g071030 61.141 561 173 5 145 661 189 748 0.0 712
MS.gene026348.t1 MTR_8g070075 56.149 618 199 7 102 658 152 758 0.0 705
MS.gene026348.t1 MTR_6g044690 57.118 576 200 12 105 649 5 564 0.0 626
MS.gene026348.t1 MTR_0376s0030 54.811 582 216 11 114 662 11 578 0.0 597
MS.gene026348.t1 MTR_6g043960 54.864 514 173 10 163 649 1 482 0.0 531
MS.gene026348.t1 MTR_2g054460 52.115 520 164 12 156 649 4 464 9.83e-172 498
MS.gene026348.t1 MTR_7g088370 41.400 500 260 14 161 649 203 680 4.50e-113 355
MS.gene026348.t1 MTR_5g013420 36.271 590 334 17 79 654 504 1065 1.96e-107 350
MS.gene026348.t1 MTR_5g013420 36.271 590 334 17 79 654 504 1065 1.99e-107 350
MS.gene026348.t1 MTR_3g011440 37.897 504 286 11 161 648 141 633 2.16e-107 339
MS.gene026348.t1 MTR_8g042750 39.960 503 279 14 161 650 167 659 3.50e-105 334
MS.gene026348.t1 MTR_4g010830 37.302 504 286 11 161 654 473 956 5.60e-100 328
MS.gene026348.t1 MTR_4g010550 37.161 479 237 14 187 654 15 440 2.11e-91 291
MS.gene026348.t1 MTR_6g061270 38.509 483 269 13 192 654 196 670 1.05e-88 291
MS.gene026348.t1 MTR_7g084090 38.763 485 266 14 192 654 258 733 3.68e-88 291
MS.gene026348.t1 MTR_6g061200 36.842 475 259 14 192 654 196 641 1.37e-79 266
MS.gene026348.t1 MTR_7g084090 41.551 361 190 11 192 538 258 611 3.39e-71 243
MS.gene026348.t1 MTR_1g048950 32.341 504 285 12 160 647 44 507 8.67e-58 205
MS.gene026348.t1 MTR_7g445710 30.103 485 237 16 195 653 110 518 2.14e-52 189
MS.gene026348.t1 MTR_7g445530 30.063 479 233 16 195 647 110 512 8.75e-52 187
MS.gene026348.t1 MTR_7g450730 30.472 466 229 14 195 655 107 482 1.56e-49 181
MS.gene026348.t1 MTR_7g052480 32.051 312 198 8 355 656 227 534 5.01e-39 152
MS.gene026348.t1 MTR_6g044000 45.833 120 47 1 163 264 1 120 7.44e-29 113
MS.gene026348.t1 MTR_6g044000 77.358 53 12 0 597 649 117 169 1.14e-21 92.8
MS.gene026348.t1 MTR_7g445600 32.667 150 64 2 498 647 243 355 1.78e-21 97.1
MS.gene026348.t1 MTR_7g445600 36.508 126 69 3 195 320 106 220 5.47e-15 77.4
MS.gene026348.t1 MTR_5g098680 37.179 156 85 3 220 369 267 415 3.98e-19 92.0
MS.gene026348.t1 MTR_8g078490 27.208 283 159 8 399 647 960 1229 5.53e-19 92.4
MS.gene026348.t1 MTR_1g080340 27.070 314 156 12 399 655 269 566 9.07e-19 90.9
MS.gene026348.t1 MTR_4g132610 28.804 184 117 3 466 647 852 1023 6.71e-18 89.0
MS.gene026348.t1 MTR_1g069570 24.839 310 163 10 399 653 430 724 7.23e-18 88.2
MS.gene026348.t1 MTR_5g018850 30.667 225 119 7 445 648 1274 1482 9.55e-18 88.6
MS.gene026348.t1 MTR_8g078505 29.508 183 115 4 466 647 346 515 1.41e-17 86.7
MS.gene026348.t1 MTR_8g102580 34.615 156 89 3 220 369 261 409 1.48e-17 87.0
MS.gene026348.t1 MTR_6g027690 27.027 222 135 6 443 662 215 411 5.60e-17 84.3
MS.gene026348.t1 MTR_7g098390 24.679 312 151 13 399 649 388 676 3.94e-15 79.7
MS.gene026348.t1 MTR_7g445810 34.591 159 84 6 195 348 123 266 7.69e-14 72.8
MS.gene026348.t1 MTR_3g114910 32.000 150 91 3 220 369 187 325 1.01e-13 73.9
MS.gene026348.t1 MTR_1g069570 25.191 262 127 9 399 606 430 676 1.80e-13 74.3
MS.gene026348.t1 MTR_1g064770 43.750 64 35 1 478 540 28 91 3.90e-12 63.2
MS.gene026348.t1 MTR_4g131630 53.846 52 21 1 594 645 1 49 6.30e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026348.t1 AT4G13460 54.669 664 271 11 10 662 6 650 0.0 703
MS.gene026348.t1 AT4G13460 54.669 664 271 11 10 662 6 650 0.0 703
MS.gene026348.t1 AT2G33290 52.061 655 269 10 21 662 29 651 0.0 671
MS.gene026348.t1 AT2G33290 52.061 655 269 10 21 662 29 651 0.0 671
MS.gene026348.t1 AT2G33290 52.061 655 269 10 21 662 29 651 0.0 671
MS.gene026348.t1 AT2G33290 52.061 655 269 10 21 662 29 651 0.0 671
MS.gene026348.t1 AT5G04940 38.889 504 277 12 161 650 155 641 2.84e-103 329
MS.gene026348.t1 AT5G04940 38.889 504 277 12 161 650 155 641 2.84e-103 329
MS.gene026348.t1 AT1G73100 40.215 465 255 12 196 650 189 640 2.75e-99 318
MS.gene026348.t1 AT2G22740 36.328 512 293 9 161 654 270 766 1.43e-96 315
MS.gene026348.t1 AT2G22740 36.328 512 293 9 161 654 270 766 1.43e-96 315
MS.gene026348.t1 AT2G35160 36.260 524 274 12 148 654 254 734 1.55e-95 311
MS.gene026348.t1 AT2G35160 36.260 524 274 12 148 654 290 770 2.54e-95 311
MS.gene026348.t1 AT2G35160 36.260 524 274 12 148 654 290 770 2.54e-95 311
MS.gene026348.t1 AT2G35160 36.260 524 274 12 148 654 290 770 2.54e-95 311
MS.gene026348.t1 AT2G35160 36.260 524 274 12 148 654 290 770 2.54e-95 311
MS.gene026348.t1 AT5G13960 36.253 491 273 16 192 656 126 602 3.18e-87 285
MS.gene026348.t1 AT5G13960 36.383 470 262 14 212 656 11 468 4.12e-86 278
MS.gene026348.t1 AT1G17770 37.124 466 269 12 197 650 209 662 2.33e-80 269
MS.gene026348.t1 AT2G24740 36.454 502 283 14 161 657 262 732 2.43e-76 259
MS.gene026348.t1 AT5G47150 36.331 278 161 7 94 369 61 324 1.98e-35 137
MS.gene026348.t1 AT5G47160 37.674 215 122 6 154 365 202 407 1.03e-28 119
MS.gene026348.t1 AT5G47160 37.674 215 122 6 154 365 202 407 1.03e-28 119
MS.gene026348.t1 AT5G47160 37.674 215 122 6 154 365 202 407 1.03e-28 119
MS.gene026348.t1 AT2G05900 36.257 171 104 3 257 427 26 191 2.87e-21 95.5
MS.gene026348.t1 AT2G23740 32.632 190 104 6 466 648 1184 1356 3.69e-21 99.4
MS.gene026348.t1 AT2G23740 32.632 190 104 6 466 648 1184 1356 3.69e-21 99.4
MS.gene026348.t1 AT2G23740 32.632 190 104 6 466 648 1184 1356 3.69e-21 99.4
MS.gene026348.t1 AT2G23740 32.632 190 104 6 466 648 1177 1349 3.81e-21 99.4
MS.gene026348.t1 AT3G03750 29.808 208 120 6 442 648 141 323 2.21e-18 87.8
MS.gene026348.t1 AT1G57820 35.669 157 87 4 220 369 277 426 5.72e-18 88.6
MS.gene026348.t1 AT1G57820 35.669 157 87 4 220 369 277 426 5.72e-18 88.6
MS.gene026348.t1 AT1G57820 35.065 154 89 4 220 369 277 423 7.12e-17 85.1
MS.gene026348.t1 AT3G04380 23.856 306 163 10 399 649 130 420 6.92e-16 81.3
MS.gene026348.t1 AT3G04380 23.856 306 163 10 399 649 146 436 8.72e-16 80.9
MS.gene026348.t1 AT3G04380 23.856 306 163 10 399 649 146 436 8.74e-16 80.9
MS.gene026348.t1 AT3G04380 23.856 306 163 10 399 649 119 409 8.91e-16 80.9
MS.gene026348.t1 AT3G04380 23.856 306 163 10 399 649 103 393 1.05e-15 80.5
MS.gene026348.t1 AT3G04380 23.856 306 163 10 399 649 119 409 1.16e-15 80.5
MS.gene026348.t1 AT5G39550 33.121 157 91 4 220 369 262 411 5.77e-15 79.0
MS.gene026348.t1 AT1G04050 25.076 331 160 11 387 649 387 697 7.81e-15 78.6
MS.gene026348.t1 AT1G04050 25.076 331 160 11 387 649 387 697 7.81e-15 78.6
MS.gene026348.t1 AT1G04050 25.076 331 160 11 387 649 283 593 8.35e-15 78.6
MS.gene026348.t1 AT2G23740 33.108 148 75 6 466 606 1184 1314 1.43e-14 78.2
MS.gene026348.t1 AT2G23740 33.108 148 75 6 466 606 1184 1314 1.43e-14 78.2
MS.gene026348.t1 AT1G66040 33.566 143 82 3 220 356 262 397 8.60e-14 75.1
MS.gene026348.t1 AT1G66050 32.867 143 83 2 220 356 262 397 9.65e-14 75.1
MS.gene026348.t1 AT5G43990 27.011 174 109 4 478 649 503 660 3.52e-13 73.2
MS.gene026348.t1 AT5G43990 27.011 174 109 4 478 649 503 660 3.52e-13 73.2
MS.gene026348.t1 AT5G43990 27.011 174 109 4 478 649 503 660 3.52e-13 73.2
MS.gene026348.t1 AT5G43990 27.011 174 109 4 478 649 503 660 3.52e-13 73.2
MS.gene026348.t1 AT5G43990 27.011 174 109 4 478 649 526 683 4.32e-13 73.2
MS.gene026348.t1 AT5G43990 27.011 174 109 4 478 649 526 683 4.32e-13 73.2
MS.gene026348.t1 AT5G43990 27.011 174 109 4 478 649 546 703 4.97e-13 72.8
MS.gene026348.t1 AT5G43990 27.011 174 109 4 478 649 546 703 4.97e-13 72.8
MS.gene026348.t1 AT5G43990 27.011 174 109 4 478 649 523 680 5.08e-13 72.8
MS.gene026348.t1 AT5G43990 27.011 174 109 4 478 649 523 680 5.08e-13 72.8
MS.gene026348.t1 AT5G43990 27.011 174 109 4 478 649 523 680 5.08e-13 72.8
MS.gene026348.t1 AT5G43990 27.011 174 109 4 478 649 523 680 5.08e-13 72.8
MS.gene026348.t1 AT5G43990 27.011 174 109 4 478 649 434 591 5.14e-13 72.8
MS.gene026348.t1 AT5G43990 27.586 174 108 4 478 649 454 611 6.89e-13 72.4
MS.gene026348.t1 AT5G43990 27.586 174 108 4 478 649 454 611 6.89e-13 72.4
MS.gene026348.t1 AT5G43990 27.586 174 108 4 478 649 454 611 6.89e-13 72.4
MS.gene026348.t1 AT5G43990 27.586 174 108 4 478 649 454 611 6.89e-13 72.4
MS.gene026348.t1 AT1G57800 31.646 158 93 5 220 369 289 439 3.00e-12 70.5
MS.gene026348.t1 AT1G57800 32.857 140 80 4 220 352 289 421 7.48e-12 68.9

Find 181 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTACTAGTGTGATTGTTTC+TGG 0.079078 1.4:+19950035 MS.gene026348:CDS
CTAGGTTCAGACTTAGATTT+AGG 0.191105 1.4:-19949404 None:intergenic
TTAGATTTAGGTTTAGATTT+TGG 0.203811 1.4:-19949392 None:intergenic
CGGAACCGTGTTACGCAAAA+AGG 0.220274 1.4:+19950737 MS.gene026348:CDS
ACCGGATCAGCGTCAGTTTC+GGG 0.257025 1.4:+19949716 MS.gene026348:CDS
TTATGATTCGGTTCGTGTTT+TGG 0.278166 1.4:+19949764 MS.gene026348:CDS
CTTGCATCTGCATTTGCATC+TGG 0.286549 1.4:-19949576 None:intergenic
AGAAGCCCGCTTTGACTATA+AGG 0.293128 1.4:-19950653 None:intergenic
GGGAGACTGGTTGGGGAGTT+AGG 0.302694 1.4:+19950792 MS.gene026348:CDS
TACCGGATCAGCGTCAGTTT+CGG 0.304741 1.4:+19949715 MS.gene026348:CDS
GTATCCATCAATTCCTCCTT+TGG 0.310358 1.4:+19950997 MS.gene026348:CDS
AGAGAGTTCAGCCTTGATTA+TGG 0.322787 1.4:+19951175 MS.gene026348:CDS
GCAAGCCACATTCCTCATTC+TGG 0.324681 1.4:-19951038 None:intergenic
GGTTGGAAGTGTTTAGATCT+AGG 0.329004 1.4:+19950771 MS.gene026348:CDS
AGGATTGTTGGCGCGATTCC+TGG 0.334668 1.4:+19949891 MS.gene026348:CDS
CGGCGTCGACGCCGCCAAAA+TGG 0.341222 1.4:+19949479 MS.gene026348:CDS
TCGGAAAATCTCCGCCATTT+TGG 0.341308 1.4:-19949493 None:intergenic
GATGTTGGTAAAAGTGGTTT+TGG 0.352738 1.4:+19950302 MS.gene026348:CDS
CTCTTTGTGTTTCATGAAAG+TGG 0.353540 1.4:+19950554 MS.gene026348:CDS
GTATGGAGCGTGTCGGTATA+AGG 0.360786 1.4:+19950462 MS.gene026348:CDS
GAAGGTACTTCTTCGACTTC+GGG 0.363101 1.4:+19950230 MS.gene026348:CDS
AGCTGGGATTGATTATTTGC+CGG 0.365503 1.4:+19949983 MS.gene026348:CDS
ATGGAGAGGAGTATGCATTA+TGG 0.365705 1.4:+19950173 MS.gene026348:CDS
TTGTGTGTGGTTGGTTTACA+TGG 0.365764 1.4:+19949951 MS.gene026348:CDS
GGCGGCGTCGACGCCGACGC+CGG 0.369583 1.4:-19949472 None:intergenic
GTCAGTTTCGGGACGTTGTT+AGG 0.371088 1.4:+19949727 MS.gene026348:CDS
TTTAAGTTATGGAGGATTGA+TGG 0.371705 1.4:+19950335 MS.gene026348:CDS
TAGATCTAGGGAGACTGGTT+GGG 0.375144 1.4:+19950784 MS.gene026348:CDS
TGCATTATGGGATTGAGGTT+AGG 0.376465 1.4:+19950186 MS.gene026348:CDS
GCTGATGGAAATGTTTGGTT+TGG 0.379538 1.4:-19949270 None:intergenic
GCCGACGCCGGCGCCGGTGG+TGG 0.380151 1.4:-19949460 None:intergenic
GTTTGGATTATGAGGATTCA+TGG 0.380762 1.4:-19949253 None:intergenic
GCATTATGGGATTGAGGTTA+GGG 0.381831 1.4:+19950187 MS.gene026348:CDS
TTCAAACAGGTGGACGGAAT+GGG 0.394764 1.4:+19950934 MS.gene026348:CDS
TGAAGGTACTTCTTCGACTT+CGG 0.398545 1.4:+19950229 MS.gene026348:CDS
ATGTTTATGATGGTTTGTAT+AGG 0.399652 1.4:+19950261 MS.gene026348:CDS
GTCGACGCCGACGCCGGCGC+CGG 0.402932 1.4:-19949466 None:intergenic
GACGAGGATGGTTTATGATT+CGG 0.406490 1.4:+19949752 MS.gene026348:CDS
AATGACGGATGTTGGGTTAC+CGG 0.408229 1.4:+19949698 MS.gene026348:CDS
TCATCCCGAGGCTCTTGCTT+CGG 0.409571 1.4:-19949324 None:intergenic
TAAATGCACCAGCTTGAATA+AGG 0.411317 1.4:-19950823 None:intergenic
TGGAGAGGAGTATGCATTAT+GGG 0.415278 1.4:+19950174 MS.gene026348:CDS
TGTATAGGATTATTGAATGC+TGG 0.416427 1.4:+19950276 MS.gene026348:CDS
GTAGTTGTCCGCCTCATTGT+CGG 0.419366 1.4:+19950717 MS.gene026348:CDS
ACAAACTGAACCATCACATT+AGG 0.419575 1.4:-19951082 None:intergenic
GCGATTCCTGGGATTTGTAT+TGG 0.420322 1.4:+19949903 MS.gene026348:CDS
GACGCCGGCGCCGGTGGTGG+TGG 0.420938 1.4:-19949457 None:intergenic
CATTTATATGCGAGTACACT+GGG 0.423254 1.4:+19950840 MS.gene026348:CDS
AAATCCAAAGGAGGAATTGA+TGG 0.424705 1.4:-19951001 None:intergenic
TCTTGATATTTCCAAGGGTA+TGG 0.441501 1.4:+19950445 MS.gene026348:CDS
ACCACCACCGGCGCCGGCGT+CGG 0.443708 1.4:+19949459 MS.gene026348:CDS
GGTAAGGTTTATGTTTATGA+TGG 0.452304 1.4:+19950251 MS.gene026348:CDS
GATTGATGGTCAAGCTAAGA+TGG 0.453129 1.4:+19950349 MS.gene026348:CDS
GGGGTGCTGATGGAAATGTT+TGG 0.454594 1.4:-19949275 None:intergenic
AATCGCGATAAGAGGATTGT+TGG 0.454649 1.4:+19949879 MS.gene026348:CDS
GTTGGAAGTGTTTAGATCTA+GGG 0.456470 1.4:+19950772 MS.gene026348:CDS
GTGTTTAGATCTAGGGAGAC+TGG 0.456502 1.4:+19950779 MS.gene026348:CDS
TGTGGTTGAATCGCGATAAG+AGG 0.456612 1.4:+19949871 MS.gene026348:CDS
CTTGTTCGAATGACGGATGT+TGG 0.459771 1.4:+19949690 MS.gene026348:CDS
GCTATTGTTCCGGTTTCTGC+TGG 0.463618 1.4:+19949600 MS.gene026348:CDS
CTCTTGATGTATCCAGAATG+AGG 0.465074 1.4:+19951026 MS.gene026348:CDS
GATTGTTTCTGGTGGGTATG+AGG 0.465601 1.4:+19950046 MS.gene026348:CDS
TTGTTCGAATGACGGATGTT+GGG 0.470012 1.4:+19949691 MS.gene026348:CDS
AATGTTTGGTTTGGATTATG+AGG 0.470379 1.4:-19949261 None:intergenic
AGATCTAGGGAGACTGGTTG+GGG 0.471997 1.4:+19950785 MS.gene026348:CDS
GTTATTATTTACTCGGGTCA+TGG 0.472036 1.4:+19950089 MS.gene026348:CDS
TACTTCTTCGACTTCGGGTA+AGG 0.474074 1.4:+19950235 MS.gene026348:CDS
TGGTTTGATGTTGGTAAAAG+TGG 0.475144 1.4:+19950296 MS.gene026348:CDS
GCCCTTGAGAAACAAAATCT+AGG 0.476619 1.4:-19949422 None:intergenic
GAACAACCAACCACCACCAC+CGG 0.476859 1.4:+19949447 MS.gene026348:CDS
AGGCGATGTTATTATTTACT+CGG 0.481383 1.4:+19950082 MS.gene026348:CDS
TCCCGAAACTGACGCTGATC+CGG 0.482112 1.4:-19949717 None:intergenic
GGATTGTTGGCGCGATTCCT+GGG 0.482675 1.4:+19949892 MS.gene026348:CDS
TTGGCTATGGCTGAAGAAGA+AGG 0.484128 1.4:+19949783 MS.gene026348:CDS
AGGATGGAGTTGTGTGTGGT+TGG 0.488005 1.4:+19949942 MS.gene026348:CDS
CCGGCGCCGGTGGTGGTGGT+TGG 0.488719 1.4:-19949453 None:intergenic
GAGTATGCATTATGGGATTG+AGG 0.491128 1.4:+19950181 MS.gene026348:CDS
GCTGGGATTGATTATTTGCC+GGG 0.497020 1.4:+19949984 MS.gene026348:CDS
GCATTTATATGCGAGTACAC+TGG 0.498857 1.4:+19950839 MS.gene026348:CDS
TTTCAAACAGGTGGACGGAA+TGG 0.498875 1.4:+19950933 MS.gene026348:CDS
TACACCGAAGCAAGAGCCTC+GGG 0.501130 1.4:+19949320 MS.gene026348:CDS
ACCGACACGCTCCATACCCT+TGG 0.501891 1.4:-19950456 None:intergenic
GGCGATGTTATTATTTACTC+GGG 0.502452 1.4:+19950083 MS.gene026348:CDS
AATTTATCTCTGATTCAAGA+TGG 0.504650 1.4:+19950959 MS.gene026348:CDS
GACGCCGACGCCGGCGCCGG+TGG 0.505428 1.4:-19949463 None:intergenic
TGCCCCTCCTCCTGCTGGTG+AGG 0.508629 1.4:+19949641 MS.gene026348:CDS
CATGAAAGTGGGAATGCGAC+GGG 0.511204 1.4:+19950566 MS.gene026348:CDS
AAAAGGGCTTAAACATAGGT+TGG 0.511958 1.4:+19950754 MS.gene026348:CDS
AATAATGCCCCTCCTCCTGC+TGG 0.512692 1.4:+19949636 MS.gene026348:CDS
TATATCTCTTGATATTTCCA+AGG 0.512997 1.4:+19950439 MS.gene026348:CDS
TCACATTATGCTGTTTGCGA+TGG 0.516973 1.4:+19951135 MS.gene026348:CDS
TCTTTGTGTTTCATGAAAGT+GGG 0.518767 1.4:+19950555 MS.gene026348:CDS
TTACTCGGGTCATGGTGGAC+AGG 0.519127 1.4:+19950097 MS.gene026348:CDS
ATTCGCGGTGTGAGGTATGA+AGG 0.522477 1.4:+19950212 MS.gene026348:CDS
TTATTCTCACCAGCAGAAAC+CGG 0.525158 1.4:-19949609 None:intergenic
CTAGTGTGATTGTTTCTGGT+GGG 0.526236 1.4:+19950039 MS.gene026348:CDS
AGCATAGGAAAAGGAATACC+AGG 0.526910 1.4:-19949297 None:intergenic
GGATATTGAAATTCTTGCGT+TGG 0.527882 1.4:-19949348 None:intergenic
ACTAGTGTGATTGTTTCTGG+TGG 0.533046 1.4:+19950038 MS.gene026348:CDS
TACATGGTCAACCACAAGCT+GGG 0.533174 1.4:+19949967 MS.gene026348:CDS
ACTTTCCTTATAGTCAAAGC+GGG 0.536291 1.4:+19950648 MS.gene026348:CDS
ATTGAATGCTGGTTTGATGT+TGG 0.540002 1.4:+19950287 MS.gene026348:CDS
TCAAGGCTGAACTCTCTCAA+TGG 0.540411 1.4:-19951169 None:intergenic
ACAATCACACTAGTAGCAAT+CGG 0.540891 1.4:-19950029 None:intergenic
TTAGATCTAGGGAGACTGGT+TGG 0.541898 1.4:+19950783 MS.gene026348:CDS
CCAACCACCACCACCGGCGC+CGG 0.541973 1.4:+19949453 MS.gene026348:CDS
ATATCTCTTGATATTTCCAA+GGG 0.542298 1.4:+19950440 MS.gene026348:CDS
TCATTGGACCTTATTCAAGC+TGG 0.545200 1.4:+19950815 MS.gene026348:CDS
TTCATCAGAAGCTGGAAGGT+GGG 0.546457 1.4:+19950141 MS.gene026348:CDS
TTCGGTGTAAGCATAGGAAA+AGG 0.548026 1.4:-19949306 None:intergenic
TTCCGACGCTCATTCACTGA+AGG 0.548549 1.4:+19949510 MS.gene026348:CDS
TGGTTGGGGAGTTAGGTCAT+TGG 0.553990 1.4:+19950799 MS.gene026348:CDS
TATTAATGAATCACCACTCA+TGG 0.554494 1.4:-19950897 None:intergenic
CAAAGCGGGCTTCTCTTGAA+AGG 0.554951 1.4:+19950662 MS.gene026348:CDS
GCTTCTCTTGAAAGGAAAGC+CGG 0.556611 1.4:+19950670 MS.gene026348:CDS
AACCTCACCAGCAGGAGGAG+GGG 0.558398 1.4:-19949643 None:intergenic
ATTGATGGTCAAGCTAAGAT+GGG 0.559091 1.4:+19950350 MS.gene026348:CDS
AAGAGTGGAAAAGTAGCCCT+AGG 0.561014 1.4:-19950536 None:intergenic
TTTCATCAGAAGCTGGAAGG+TGG 0.563122 1.4:+19950140 MS.gene026348:CDS
ATAACCTCACCAGCAGGAGG+AGG 0.563671 1.4:-19949645 None:intergenic
CGTTGTTAGGAGGACGAGGA+TGG 0.564376 1.4:+19949740 MS.gene026348:CDS
TTACATGGTCAACCACAAGC+TGG 0.564688 1.4:+19949966 MS.gene026348:CDS
TCATGAAAGTGGGAATGCGA+CGG 0.565558 1.4:+19950565 MS.gene026348:CDS
TCAAACAGGTGGACGGAATG+GGG 0.569248 1.4:+19950935 MS.gene026348:CDS
GTGGACAGGATAAGAATTCG+AGG 0.571781 1.4:+19950111 MS.gene026348:CDS
CCGGGGAGTATGAGTTCGAA+TGG 0.571907 1.4:+19950002 MS.gene026348:CDS
ATTGAGGTTAGGGTTATTCG+CGG 0.572582 1.4:+19950197 MS.gene026348:CDS
ACAGGATAAGAATTCGAGGC+AGG 0.572812 1.4:+19950115 MS.gene026348:CDS
AGATGCAAGAGCTATTGTTC+CGG 0.573112 1.4:+19949590 MS.gene026348:CDS
CATCAGCTACACCATAATCA+AGG 0.573838 1.4:-19951186 None:intergenic
CGATGAGGAGTCGAGGATTG+TGG 0.574012 1.4:+19949853 MS.gene026348:CDS
TGGCTATGGCTGAAGAAGAA+GGG 0.574030 1.4:+19949784 MS.gene026348:CDS
GTATAGGATGGAGTTGTGTG+TGG 0.574388 1.4:+19949938 MS.gene026348:CDS
GGCTGAACTCTCTCAATGGA+GGG 0.575518 1.4:-19951165 None:intergenic
GGAAGGTGGGAATCTTGCTA+TGG 0.576684 1.4:+19950154 MS.gene026348:CDS
TAAAGAGCTTGTTCGAATGA+CGG 0.576987 1.4:+19949683 MS.gene026348:CDS
TCTTGCTTCGGTGTAAGCAT+AGG 0.581620 1.4:-19949312 None:intergenic
ACATTCATAACCTCACCAGC+AGG 0.581786 1.4:-19949651 None:intergenic
TAGCTCAACTCCTAATGTGA+TGG 0.586959 1.4:+19951072 MS.gene026348:CDS
TAACCTCACCAGCAGGAGGA+GGG 0.592169 1.4:-19949644 None:intergenic
CTTTCATGAAACACAAAGAG+TGG 0.592723 1.4:-19950551 None:intergenic
TTACACCGAAGCAAGAGCCT+CGG 0.592731 1.4:+19949319 MS.gene026348:CDS
GGGACGTTGTTAGGAGGACG+AGG 0.594825 1.4:+19949736 MS.gene026348:CDS
AGGCTGAACTCTCTCAATGG+AGG 0.596120 1.4:-19951166 None:intergenic
CGTCGACGCCGCCAAAATGG+CGG 0.596135 1.4:+19949482 MS.gene026348:CDS
AGTTTCGGGACGTTGTTAGG+AGG 0.598043 1.4:+19949730 MS.gene026348:CDS
TCAAGAGATATAACACACAT+AGG 0.602330 1.4:-19950428 None:intergenic
TCTTGCGTTGGTTCATCCCG+AGG 0.604080 1.4:-19949336 None:intergenic
GGAGGAATTGATGGATACGA+AGG 0.604652 1.4:-19950992 None:intergenic
CTGGTGAGGTTATGAATGTA+CGG 0.606823 1.4:+19949655 MS.gene026348:CDS
TTAGGGTTATTCGCGGTGTG+AGG 0.610018 1.4:+19950204 MS.gene026348:CDS
ATCCTTCAGTGAATGAGCGT+CGG 0.615611 1.4:-19949512 None:intergenic
ACATCACCAATACAAATCCC+AGG 0.617403 1.4:-19949909 None:intergenic
TCAGAAATTATGACCATGAG+TGG 0.618462 1.4:+19950884 MS.gene026348:CDS
GACTTTCCTTATAGTCAAAG+CGG 0.621269 1.4:+19950647 MS.gene026348:CDS
ACACTGGGGTTGTTCTGACA+AGG 0.627478 1.4:+19950855 MS.gene026348:CDS
CCATTCGAACTCATACTCCC+CGG 0.628119 1.4:-19950002 None:intergenic
TATGAGGATGATGTTGATGA+AGG 0.628797 1.4:+19950062 MS.gene026348:CDS
GTGGGAATCTTGCTATGGAG+AGG 0.630151 1.4:+19950159 MS.gene026348:CDS
GGAATTGATGGATACGAAGG+AGG 0.638458 1.4:-19950989 None:intergenic
ATCGCAAACAGCATAATGTG+AGG 0.644756 1.4:-19951133 None:intergenic
AAATAATCAATCCCAGCTTG+TGG 0.646654 1.4:-19949978 None:intergenic
GTAGCTGATGATGAGTTGAC+TGG 0.647668 1.4:+19951199 MS.gene026348:CDS
CACATAGGTTTATAACACAA+AGG 0.649300 1.4:-19950413 None:intergenic
CTGGGATTGATTATTTGCCG+GGG 0.652302 1.4:+19949985 MS.gene026348:CDS
TCGCAAACAGCATAATGTGA+GGG 0.654166 1.4:-19951132 None:intergenic
TGAAAGCTTCTACTACGATG+AGG 0.657632 1.4:+19949838 MS.gene026348:CDS
TTCATAACCTCACCAGCAGG+AGG 0.665106 1.4:-19949648 None:intergenic
TGAGGCGGACAACTACAAGA+AGG 0.667023 1.4:-19950710 None:intergenic
CACTGGGGTTGTTCTGACAA+GGG 0.671410 1.4:+19950856 MS.gene026348:CDS
ATTATTTACTCGGGTCATGG+TGG 0.676989 1.4:+19950092 MS.gene026348:CDS
TCTACTACGATGAGGAGTCG+AGG 0.680653 1.4:+19949846 MS.gene026348:CDS
ACACGGTTCCGACAATGAGG+CGG 0.681770 1.4:-19950725 None:intergenic
TCCAAGGGTATGGAGCGTGT+CGG 0.686880 1.4:+19950455 MS.gene026348:CDS
GGAAGATACTCAAAACACAT+CGG 0.687151 1.4:-19950515 None:intergenic
GCATAGGAAAAGGAATACCA+GGG 0.688262 1.4:-19949296 None:intergenic
GTAACACGGTTCCGACAATG+AGG 0.724410 1.4:-19950728 None:intergenic
CATAGGAAAAGGAATACCAG+GGG 0.729249 1.4:-19949295 None:intergenic
TGTATCCAGAATGAGGAATG+TGG 0.734351 1.4:+19951033 MS.gene026348:CDS
ATTTATATGCGAGTACACTG+GGG 0.744900 1.4:+19950841 MS.gene026348:CDS
ATAGGAAAAGGAATACCAGG+GGG 0.791262 1.4:-19949294 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTTTGTTTCATAAAAAA+TGG + chr1.4:19950620-19950639 MS.gene026348:CDS 15.0%
!!! GTGTTTTTAAGTTTAAGTTA+TGG + chr1.4:19950324-19950343 MS.gene026348:CDS 20.0%
!!! TTAGATTTAGGTTTAGATTT+TGG - chr1.4:19949395-19949414 None:intergenic 20.0%
!!! TTTTTAAGTTTAAGTTATGG+AGG + chr1.4:19950327-19950346 MS.gene026348:CDS 20.0%
! AATTTATCTCTGATTCAAGA+TGG + chr1.4:19950959-19950978 MS.gene026348:CDS 25.0%
! ATATCTCTTGATATTTCCAA+GGG + chr1.4:19950440-19950459 MS.gene026348:CDS 25.0%
! TATATCTCTTGATATTTCCA+AGG + chr1.4:19950439-19950458 MS.gene026348:CDS 25.0%
!! ATGTTTATGATGGTTTGTAT+AGG + chr1.4:19950261-19950280 MS.gene026348:CDS 25.0%
!!! AAGAAGGGAATTTTAATGTT+AGG + chr1.4:19949799-19949818 MS.gene026348:CDS 25.0%
!!! TTTAGGTTTAGATTTTGGTT+CGG - chr1.4:19949390-19949409 None:intergenic 25.0%
ACATCAAGAGAAAAATCCAA+AGG - chr1.4:19951016-19951035 None:intergenic 30.0%
AGGCGATGTTATTATTTACT+CGG + chr1.4:19950082-19950101 MS.gene026348:CDS 30.0%
CACATAGGTTTATAACACAA+AGG - chr1.4:19950416-19950435 None:intergenic 30.0%
GGTAAGGTTTATGTTTATGA+TGG + chr1.4:19950251-19950270 MS.gene026348:CDS 30.0%
TATTAATGAATCACCACTCA+TGG - chr1.4:19950900-19950919 None:intergenic 30.0%
TCAAGAGATATAACACACAT+AGG - chr1.4:19950431-19950450 None:intergenic 30.0%
TCTTTGTGTTTCATGAAAGT+GGG + chr1.4:19950555-19950574 MS.gene026348:CDS 30.0%
TGTATAGGATTATTGAATGC+TGG + chr1.4:19950276-19950295 MS.gene026348:CDS 30.0%
! TTCGGTAAATTTTCTAGGTT+AGG - chr1.4:19949372-19949391 None:intergenic 30.0%
! TTTAAGTTATGGAGGATTGA+TGG + chr1.4:19950335-19950354 MS.gene026348:CDS 30.0%
!! AACCTAGATTTTGTTTCTCA+AGG + chr1.4:19949420-19949439 MS.gene026348:CDS 30.0%
!! AATGTTTGGTTTGGATTATG+AGG - chr1.4:19949264-19949283 None:intergenic 30.0%
!! ACCTAGATTTTGTTTCTCAA+GGG + chr1.4:19949421-19949440 MS.gene026348:CDS 30.0%
!! GATGGGTAGTTTGATTTTAA+AGG + chr1.4:19950367-19950386 MS.gene026348:CDS 30.0%
!! TGATGTGTTTTTGTATAGGA+TGG + chr1.4:19949926-19949945 MS.gene026348:CDS 30.0%
!!! TTGGTGATGTGTTTTTGTAT+AGG + chr1.4:19949922-19949941 MS.gene026348:CDS 30.0%
!!! TTTGGTTCGGTAAATTTTCT+AGG - chr1.4:19949377-19949396 None:intergenic 30.0%
AAAAGGGCTTAAACATAGGT+TGG + chr1.4:19950754-19950773 MS.gene026348:CDS 35.0%
AAATAATCAATCCCAGCTTG+TGG - chr1.4:19949981-19950000 None:intergenic 35.0%
AAATCCAAAGGAGGAATTGA+TGG - chr1.4:19951004-19951023 None:intergenic 35.0%
ACAAACTGAACCATCACATT+AGG - chr1.4:19951085-19951104 None:intergenic 35.0%
ACAATCACACTAGTAGCAAT+CGG - chr1.4:19950032-19950051 None:intergenic 35.0%
ACTTTCCTTATAGTCAAAGC+GGG + chr1.4:19950648-19950667 MS.gene026348:CDS 35.0%
ATTTATATGCGAGTACACTG+GGG + chr1.4:19950841-19950860 MS.gene026348:CDS 35.0%
CACCTGTTTGAAAAACGATT+CGG - chr1.4:19950926-19950945 None:intergenic 35.0%
CATTTATATGCGAGTACACT+GGG + chr1.4:19950840-19950859 MS.gene026348:CDS 35.0%
CTAGGTTCAGACTTAGATTT+AGG - chr1.4:19949407-19949426 None:intergenic 35.0%
CTCTTTGTGTTTCATGAAAG+TGG + chr1.4:19950554-19950573 MS.gene026348:CDS 35.0%
CTTTCATGAAACACAAAGAG+TGG - chr1.4:19950554-19950573 None:intergenic 35.0%
GACTTTCCTTATAGTCAAAG+CGG + chr1.4:19950647-19950666 MS.gene026348:CDS 35.0%
GGAAGATACTCAAAACACAT+CGG - chr1.4:19950518-19950537 None:intergenic 35.0%
GGATATTGAAATTCTTGCGT+TGG - chr1.4:19949351-19949370 None:intergenic 35.0%
GGCGATGTTATTATTTACTC+GGG + chr1.4:19950083-19950102 MS.gene026348:CDS 35.0%
GTTATTATTTACTCGGGTCA+TGG + chr1.4:19950089-19950108 MS.gene026348:CDS 35.0%
TAAATGCACCAGCTTGAATA+AGG - chr1.4:19950826-19950845 None:intergenic 35.0%
TCAAGAGAAAAATCCAAAGG+AGG - chr1.4:19951013-19951032 None:intergenic 35.0%
TCAGAAATTATGACCATGAG+TGG + chr1.4:19950884-19950903 MS.gene026348:CDS 35.0%
TCTTGATATTTCCAAGGGTA+TGG + chr1.4:19950445-19950464 MS.gene026348:CDS 35.0%
! ATTGATGGTCAAGCTAAGAT+GGG + chr1.4:19950350-19950369 MS.gene026348:CDS 35.0%
! TAAAGAGCTTGTTCGAATGA+CGG + chr1.4:19949683-19949702 MS.gene026348:CDS 35.0%
! TATGAGGATGATGTTGATGA+AGG + chr1.4:19950062-19950081 MS.gene026348:CDS 35.0%
! TGGTTTGATGTTGGTAAAAG+TGG + chr1.4:19950296-19950315 MS.gene026348:CDS 35.0%
!! ATCCGAATCGTTTTTCAAAC+AGG + chr1.4:19950921-19950940 MS.gene026348:CDS 35.0%
!! ATTGAATGCTGGTTTGATGT+TGG + chr1.4:19950287-19950306 MS.gene026348:CDS 35.0%
!! GATGTTGGTAAAAGTGGTTT+TGG + chr1.4:19950302-19950321 MS.gene026348:CDS 35.0%
!! GGGTAGTTTGATTTTAAAGG+AGG + chr1.4:19950370-19950389 MS.gene026348:CDS 35.0%
!! GTGTTTTGAGTATCTTCCTA+GGG + chr1.4:19950520-19950539 MS.gene026348:CDS 35.0%
!! GTTGGAAGTGTTTAGATCTA+GGG + chr1.4:19950772-19950791 MS.gene026348:CDS 35.0%
!! TGTGTTTTGAGTATCTTCCT+AGG + chr1.4:19950519-19950538 MS.gene026348:CDS 35.0%
!!! TAAAGGAGGCTTTTTTGCTT+AGG + chr1.4:19950384-19950403 MS.gene026348:CDS 35.0%
!!! TTATGATTCGGTTCGTGTTT+TGG + chr1.4:19949764-19949783 MS.gene026348:CDS 35.0%
AATCGCGATAAGAGGATTGT+TGG + chr1.4:19949879-19949898 MS.gene026348:CDS 40.0%
ACATCACCAATACAAATCCC+AGG - chr1.4:19949912-19949931 None:intergenic 40.0%
AGAGAGTTCAGCCTTGATTA+TGG + chr1.4:19951175-19951194 MS.gene026348:CDS 40.0%
AGATGCAAGAGCTATTGTTC+CGG + chr1.4:19949590-19949609 MS.gene026348:CDS 40.0%
AGCATAGGAAAAGGAATACC+AGG - chr1.4:19949300-19949319 None:intergenic 40.0%
AGCTGGGATTGATTATTTGC+CGG + chr1.4:19949983-19950002 MS.gene026348:CDS 40.0%
ATAGGAAAAGGAATACCAGG+GGG - chr1.4:19949297-19949316 None:intergenic 40.0%
ATCGCAAACAGCATAATGTG+AGG - chr1.4:19951136-19951155 None:intergenic 40.0%
ATTATTTACTCGGGTCATGG+TGG + chr1.4:19950092-19950111 MS.gene026348:CDS 40.0%
ATTGAGGTTAGGGTTATTCG+CGG + chr1.4:19950197-19950216 MS.gene026348:CDS 40.0%
CATAGGAAAAGGAATACCAG+GGG - chr1.4:19949298-19949317 None:intergenic 40.0%
CATCAGCTACACCATAATCA+AGG - chr1.4:19951189-19951208 None:intergenic 40.0%
CGCAAAAAGGGCTTAAACAT+AGG + chr1.4:19950750-19950769 MS.gene026348:CDS 40.0%
CTCTTGATGTATCCAGAATG+AGG + chr1.4:19951026-19951045 MS.gene026348:CDS 40.0%
CTGGTGAGGTTATGAATGTA+CGG + chr1.4:19949655-19949674 MS.gene026348:CDS 40.0%
GACGAGGATGGTTTATGATT+CGG + chr1.4:19949752-19949771 MS.gene026348:CDS 40.0%
GAGTATGCATTATGGGATTG+AGG + chr1.4:19950181-19950200 MS.gene026348:CDS 40.0%
GCATAGGAAAAGGAATACCA+GGG - chr1.4:19949299-19949318 None:intergenic 40.0%
GCATTATGGGATTGAGGTTA+GGG + chr1.4:19950187-19950206 MS.gene026348:CDS 40.0%
GCATTTATATGCGAGTACAC+TGG + chr1.4:19950839-19950858 MS.gene026348:CDS 40.0%
GCCCTTGAGAAACAAAATCT+AGG - chr1.4:19949425-19949444 None:intergenic 40.0%
GCTACTAGTGTGATTGTTTC+TGG + chr1.4:19950035-19950054 MS.gene026348:CDS 40.0%
GCTGATGGAAATGTTTGGTT+TGG - chr1.4:19949273-19949292 None:intergenic 40.0%
GGACCACATTCAAAAATGAC+CGG - chr1.4:19950692-19950711 None:intergenic 40.0%
GTATCCATCAATTCCTCCTT+TGG + chr1.4:19950997-19951016 MS.gene026348:CDS 40.0%
TAGCTCAACTCCTAATGTGA+TGG + chr1.4:19951072-19951091 MS.gene026348:CDS 40.0%
TCATTGGACCTTATTCAAGC+TGG + chr1.4:19950815-19950834 MS.gene026348:CDS 40.0%
TCGCAAACAGCATAATGTGA+GGG - chr1.4:19951135-19951154 None:intergenic 40.0%
TGAAAGCTTCTACTACGATG+AGG + chr1.4:19949838-19949857 MS.gene026348:CDS 40.0%
TGCATTATGGGATTGAGGTT+AGG + chr1.4:19950186-19950205 MS.gene026348:CDS 40.0%
TGTATCCAGAATGAGGAATG+TGG + chr1.4:19951033-19951052 MS.gene026348:CDS 40.0%
TTATTCTCACCAGCAGAAAC+CGG - chr1.4:19949612-19949631 None:intergenic 40.0%
! ATGGAGAGGAGTATGCATTA+TGG + chr1.4:19950173-19950192 MS.gene026348:CDS 40.0%
! GATTGATGGTCAAGCTAAGA+TGG + chr1.4:19950349-19950368 MS.gene026348:CDS 40.0%
! GTCAATGTGAGAGCTTTTGT+GGG + chr1.4:19950591-19950610 MS.gene026348:CDS 40.0%
! GTTTTTCATCAGAAGCTGGA+AGG + chr1.4:19950137-19950156 MS.gene026348:CDS 40.0%
! TAAGCCCTTTTTGCGTAACA+CGG - chr1.4:19950745-19950764 None:intergenic 40.0%
! TCAATGTGAGAGCTTTTGTG+GGG + chr1.4:19950592-19950611 MS.gene026348:CDS 40.0%
! TCACATTATGCTGTTTGCGA+TGG + chr1.4:19951135-19951154 MS.gene026348:CDS 40.0%
! TGGAGAGGAGTATGCATTAT+GGG + chr1.4:19950174-19950193 MS.gene026348:CDS 40.0%
! TGTCAATGTGAGAGCTTTTG+TGG + chr1.4:19950590-19950609 MS.gene026348:CDS 40.0%
! TTCGGTGTAAGCATAGGAAA+AGG - chr1.4:19949309-19949328 None:intergenic 40.0%
!! ACTAGTGTGATTGTTTCTGG+TGG + chr1.4:19950038-19950057 MS.gene026348:CDS 40.0%
!! CGAATCGTTTTTCAAACAGG+TGG + chr1.4:19950924-19950943 MS.gene026348:CDS 40.0%
!! CTAGTGTGATTGTTTCTGGT+GGG + chr1.4:19950039-19950058 MS.gene026348:CDS 40.0%
!! GGTTGGAAGTGTTTAGATCT+AGG + chr1.4:19950771-19950790 MS.gene026348:CDS 40.0%
!! TCGTTTTTCAAACAGGTGGA+CGG + chr1.4:19950928-19950947 MS.gene026348:CDS 40.0%
!! TGAAGGTACTTCTTCGACTT+CGG + chr1.4:19950229-19950248 MS.gene026348:CDS 40.0%
!! TTGTGTGTGGTTGGTTTACA+TGG + chr1.4:19949951-19949970 MS.gene026348:CDS 40.0%
!! TTGTTCGAATGACGGATGTT+GGG + chr1.4:19949691-19949710 MS.gene026348:CDS 40.0%
!!! AAGCCGGTCATTTTTGAATG+TGG + chr1.4:19950686-19950705 MS.gene026348:CDS 40.0%
AAGAGTGGAAAAGTAGCCCT+AGG - chr1.4:19950539-19950558 None:intergenic 45.0%
ACAGGATAAGAATTCGAGGC+AGG + chr1.4:19950115-19950134 MS.gene026348:CDS 45.0%
ACATTCATAACCTCACCAGC+AGG - chr1.4:19949654-19949673 None:intergenic 45.0%
AGAAGCCCGCTTTGACTATA+AGG - chr1.4:19950656-19950675 None:intergenic 45.0%
ATCCTTCAGTGAATGAGCGT+CGG - chr1.4:19949515-19949534 None:intergenic 45.0%
CTGGGATTGATTATTTGCCG+GGG + chr1.4:19949985-19950004 MS.gene026348:CDS 45.0%
CTTGCATCTGCATTTGCATC+TGG - chr1.4:19949579-19949598 None:intergenic 45.0%
GCGATTCCTGGGATTTGTAT+TGG + chr1.4:19949903-19949922 MS.gene026348:CDS 45.0%
GCTGGGATTGATTATTTGCC+GGG + chr1.4:19949984-19950003 MS.gene026348:CDS 45.0%
GCTTCTCTTGAAAGGAAAGC+CGG + chr1.4:19950670-19950689 MS.gene026348:CDS 45.0%
GGAACCGTGTTACGCAAAAA+GGG + chr1.4:19950738-19950757 MS.gene026348:CDS 45.0%
GTATAGGATGGAGTTGTGTG+TGG + chr1.4:19949938-19949957 MS.gene026348:CDS 45.0%
GTGGACAGGATAAGAATTCG+AGG + chr1.4:19950111-19950130 MS.gene026348:CDS 45.0%
GTGTTTAGATCTAGGGAGAC+TGG + chr1.4:19950779-19950798 MS.gene026348:CDS 45.0%
TACATGGTCAACCACAAGCT+GGG + chr1.4:19949967-19949986 MS.gene026348:CDS 45.0%
TACTTCTTCGACTTCGGGTA+AGG + chr1.4:19950235-19950254 MS.gene026348:CDS 45.0%
TCAAGGCTGAACTCTCTCAA+TGG - chr1.4:19951172-19951191 None:intergenic 45.0%
TCATGAAAGTGGGAATGCGA+CGG + chr1.4:19950565-19950584 MS.gene026348:CDS 45.0%
TGGCTATGGCTGAAGAAGAA+GGG + chr1.4:19949784-19949803 MS.gene026348:CDS 45.0%
TGTGGTTGAATCGCGATAAG+AGG + chr1.4:19949871-19949890 MS.gene026348:CDS 45.0%
TTACATGGTCAACCACAAGC+TGG + chr1.4:19949966-19949985 MS.gene026348:CDS 45.0%
TTAGATCTAGGGAGACTGGT+TGG + chr1.4:19950783-19950802 MS.gene026348:CDS 45.0%
TTCAAACAGGTGGACGGAAT+GGG + chr1.4:19950934-19950953 MS.gene026348:CDS 45.0%
TTCATCAGAAGCTGGAAGGT+GGG + chr1.4:19950141-19950160 MS.gene026348:CDS 45.0%
TTGGCTATGGCTGAAGAAGA+AGG + chr1.4:19949783-19949802 MS.gene026348:CDS 45.0%
TTTCAAACAGGTGGACGGAA+TGG + chr1.4:19950933-19950952 MS.gene026348:CDS 45.0%
TTTCATCAGAAGCTGGAAGG+TGG + chr1.4:19950140-19950159 MS.gene026348:CDS 45.0%
! GCAGGTTTTTCATCAGAAGC+TGG + chr1.4:19950133-19950152 MS.gene026348:CDS 45.0%
! GGAATTGATGGATACGAAGG+AGG - chr1.4:19950992-19951011 None:intergenic 45.0%
! GGAGGAATTGATGGATACGA+AGG - chr1.4:19950995-19951014 None:intergenic 45.0%
! GTAGCTGATGATGAGTTGAC+TGG + chr1.4:19951199-19951218 MS.gene026348:CDS 45.0%
! TAGATCTAGGGAGACTGGTT+GGG + chr1.4:19950784-19950803 MS.gene026348:CDS 45.0%
! TCGGAAAATCTCCGCCATTT+TGG - chr1.4:19949496-19949515 None:intergenic 45.0%
! TCTTGCTTCGGTGTAAGCAT+AGG - chr1.4:19949315-19949334 None:intergenic 45.0%
!! AATGACGGATGTTGGGTTAC+CGG + chr1.4:19949698-19949717 MS.gene026348:CDS 45.0%
!! CTTGTTCGAATGACGGATGT+TGG + chr1.4:19949690-19949709 MS.gene026348:CDS 45.0%
!! GAAAATCTCCGCCATTTTGG+CGG - chr1.4:19949493-19949512 None:intergenic 45.0%
!! GAAGGTACTTCTTCGACTTC+GGG + chr1.4:19950230-19950249 MS.gene026348:CDS 45.0%
!! GATTGTTTCTGGTGGGTATG+AGG + chr1.4:19950046-19950065 MS.gene026348:CDS 45.0%
!!! TTCGGTTCGTGTTTTGGCTA+TGG + chr1.4:19949770-19949789 MS.gene026348:CDS 45.0%
ACACTGGGGTTGTTCTGACA+AGG + chr1.4:19950855-19950874 MS.gene026348:CDS 50.0%
AGGATGGAGTTGTGTGTGGT+TGG + chr1.4:19949942-19949961 MS.gene026348:CDS 50.0%
AGGCTGAACTCTCTCAATGG+AGG - chr1.4:19951169-19951188 None:intergenic 50.0%
AGTTTCGGGACGTTGTTAGG+AGG + chr1.4:19949730-19949749 MS.gene026348:CDS 50.0%
ATTCGCGGTGTGAGGTATGA+AGG + chr1.4:19950212-19950231 MS.gene026348:CDS 50.0%
CACTGGGGTTGTTCTGACAA+GGG + chr1.4:19950856-19950875 MS.gene026348:CDS 50.0%
CATGAAAGTGGGAATGCGAC+GGG + chr1.4:19950566-19950585 MS.gene026348:CDS 50.0%
CCATTCGAACTCATACTCCC+CGG - chr1.4:19950005-19950024 None:intergenic 50.0%
CGGAACCGTGTTACGCAAAA+AGG + chr1.4:19950737-19950756 MS.gene026348:CDS 50.0%
GCAAGCCACATTCCTCATTC+TGG - chr1.4:19951041-19951060 None:intergenic 50.0%
GCTATTGTTCCGGTTTCTGC+TGG + chr1.4:19949600-19949619 MS.gene026348:CDS 50.0%
GGAAGGTGGGAATCTTGCTA+TGG + chr1.4:19950154-19950173 MS.gene026348:CDS 50.0%
GGCTGAACTCTCTCAATGGA+GGG - chr1.4:19951168-19951187 None:intergenic 50.0%
GTAACACGGTTCCGACAATG+AGG - chr1.4:19950731-19950750 None:intergenic 50.0%
GTAGTTGTCCGCCTCATTGT+CGG + chr1.4:19950717-19950736 MS.gene026348:CDS 50.0%
GTCAGTTTCGGGACGTTGTT+AGG + chr1.4:19949727-19949746 MS.gene026348:CDS 50.0%
TACCGGATCAGCGTCAGTTT+CGG + chr1.4:19949715-19949734 MS.gene026348:CDS 50.0%
TCAAACAGGTGGACGGAATG+GGG + chr1.4:19950935-19950954 MS.gene026348:CDS 50.0%
TCTACTACGATGAGGAGTCG+AGG + chr1.4:19949846-19949865 MS.gene026348:CDS 50.0%
TGAGGCGGACAACTACAAGA+AGG - chr1.4:19950713-19950732 None:intergenic 50.0%
TGGTTGGGGAGTTAGGTCAT+TGG + chr1.4:19950799-19950818 MS.gene026348:CDS 50.0%
TTACACCGAAGCAAGAGCCT+CGG + chr1.4:19949319-19949338 MS.gene026348:CDS 50.0%
TTAGGGTTATTCGCGGTGTG+AGG + chr1.4:19950204-19950223 MS.gene026348:CDS 50.0%
TTCATAACCTCACCAGCAGG+AGG - chr1.4:19949651-19949670 None:intergenic 50.0%
TTCCGACGCTCATTCACTGA+AGG + chr1.4:19949510-19949529 MS.gene026348:CDS 50.0%
! AGATCTAGGGAGACTGGTTG+GGG + chr1.4:19950785-19950804 MS.gene026348:CDS 50.0%
! CAAAGCGGGCTTCTCTTGAA+AGG + chr1.4:19950662-19950681 MS.gene026348:CDS 50.0%
! GTATGGAGCGTGTCGGTATA+AGG + chr1.4:19950462-19950481 MS.gene026348:CDS 50.0%
! GTGGGAATCTTGCTATGGAG+AGG + chr1.4:19950159-19950178 MS.gene026348:CDS 50.0%
! TGTGAGAGCTTTTGTGGGGA+AGG + chr1.4:19950596-19950615 MS.gene026348:CDS 50.0%
!! GGGGTGCTGATGGAAATGTT+TGG - chr1.4:19949278-19949297 None:intergenic 50.0%
AATAATGCCCCTCCTCCTGC+TGG + chr1.4:19949636-19949655 MS.gene026348:CDS 55.0%
ACACGGTTCCGACAATGAGG+CGG - chr1.4:19950728-19950747 None:intergenic 55.0%
ACATTTCCATCAGCACCCCC+TGG + chr1.4:19949279-19949298 MS.gene026348:CDS 55.0%
ACCGGATCAGCGTCAGTTTC+GGG + chr1.4:19949716-19949735 MS.gene026348:CDS 55.0%
AGGATTGTTGGCGCGATTCC+TGG + chr1.4:19949891-19949910 MS.gene026348:CDS 55.0%
ATAACCTCACCAGCAGGAGG+AGG - chr1.4:19949648-19949667 None:intergenic 55.0%
CGATGAGGAGTCGAGGATTG+TGG + chr1.4:19949853-19949872 MS.gene026348:CDS 55.0%
CGTTGTTAGGAGGACGAGGA+TGG + chr1.4:19949740-19949759 MS.gene026348:CDS 55.0%
GAACAACCAACCACCACCAC+CGG + chr1.4:19949447-19949466 MS.gene026348:CDS 55.0%
GGATTGTTGGCGCGATTCCT+GGG + chr1.4:19949892-19949911 MS.gene026348:CDS 55.0%
TAACCTCACCAGCAGGAGGA+GGG - chr1.4:19949647-19949666 None:intergenic 55.0%
TACACCGAAGCAAGAGCCTC+GGG + chr1.4:19949320-19949339 MS.gene026348:CDS 55.0%
TCATCCCGAGGCTCTTGCTT+CGG - chr1.4:19949327-19949346 None:intergenic 55.0%
TCCCGAAACTGACGCTGATC+CGG - chr1.4:19949720-19949739 None:intergenic 55.0%
! CCGGGGAGTATGAGTTCGAA+TGG + chr1.4:19950002-19950021 MS.gene026348:CDS 55.0%
! TCCAAGGGTATGGAGCGTGT+CGG + chr1.4:19950455-19950474 MS.gene026348:CDS 55.0%
!! TCTTGCGTTGGTTCATCCCG+AGG - chr1.4:19949339-19949358 None:intergenic 55.0%
!! TTACTCGGGTCATGGTGGAC+AGG + chr1.4:19950097-19950116 MS.gene026348:CDS 55.0%
AACCTCACCAGCAGGAGGAG+GGG - chr1.4:19949646-19949665 None:intergenic 60.0%
ACCGACACGCTCCATACCCT+TGG - chr1.4:19950459-19950478 None:intergenic 60.0%
GGGACGTTGTTAGGAGGACG+AGG + chr1.4:19949736-19949755 MS.gene026348:CDS 60.0%
! GGGAGACTGGTTGGGGAGTT+AGG + chr1.4:19950792-19950811 MS.gene026348:CDS 60.0%
!! GGAATACCAGGGGGTGCTGA+TGG - chr1.4:19949288-19949307 None:intergenic 60.0%
CGTCGACGCCGCCAAAATGG+CGG + chr1.4:19949482-19949501 MS.gene026348:CDS 65.0%
TGCCCCTCCTCCTGCTGGTG+AGG + chr1.4:19949641-19949660 MS.gene026348:CDS 70.0%
!! CGGCGTCGACGCCGCCAAAA+TGG + chr1.4:19949479-19949498 MS.gene026348:CDS 70.0%
CCAACCACCACCACCGGCGC+CGG + chr1.4:19949453-19949472 MS.gene026348:CDS 75.0%
!!! ACCACCACCGGCGCCGGCGT+CGG + chr1.4:19949459-19949478 MS.gene026348:CDS 80.0%
!!! CCGGCGCCGGTGGTGGTGGT+TGG - chr1.4:19949456-19949475 None:intergenic 80.0%
! GTCGACGCCGACGCCGGCGC+CGG - chr1.4:19949469-19949488 None:intergenic 85.0%
!!! GACGCCGGCGCCGGTGGTGG+TGG - chr1.4:19949460-19949479 None:intergenic 85.0%
!!! GGCGGCGTCGACGCCGACGC+CGG - chr1.4:19949475-19949494 None:intergenic 85.0%
!! GACGCCGACGCCGGCGCCGG+TGG - chr1.4:19949466-19949485 None:intergenic 90.0%
!!! GCCGACGCCGGCGCCGGTGG+TGG - chr1.4:19949463-19949482 None:intergenic 90.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 19949255 19951243 19949255 ID=MS.gene026348
chr1.4 mRNA 19949255 19951243 19949255 ID=MS.gene026348.t1;Parent=MS.gene026348
chr1.4 exon 19949255 19951243 19949255 ID=MS.gene026348.t1.exon1;Parent=MS.gene026348.t1
chr1.4 CDS 19949255 19951243 19949255 ID=cds.MS.gene026348.t1;Parent=MS.gene026348.t1
Gene Sequence

>MS.gene026348

ATGAATCCTCATAATCCAAACCAAACATTTCCATCAGCACCCCCTGGTATTCCTTTTCCTATGCTTACACCGAAGCAAGAGCCTCGGGATGAACCAACGCAAGAATTTCAATATCCTAACCTAGAAAATTTACCGAACCAAAATCTAAACCTAAATCTAAGTCTGAACCTAGATTTTGTTTCTCAAGGGCTAGAACAACCAACCACCACCACCGGCGCCGGCGTCGGCGTCGACGCCGCCAAAATGGCGGAGATTTTCCGACGCTCATTCACTGAAGGATTACAACGACAGATTCAAAACAATGATGCTGTTGATGAAAATCCAGATGCAAATGCAGATGCAAGAGCTATTGTTCCGGTTTCTGCTGGTGAGAATAATTACAATAATGCCCCTCCTCCTGCTGGTGAGGTTATGAATGTACGGAAACATAAAGAGCTTGTTCGAATGACGGATGTTGGGTTACCGGATCAGCGTCAGTTTCGGGACGTTGTTAGGAGGACGAGGATGGTTTATGATTCGGTTCGTGTTTTGGCTATGGCTGAAGAAGAAGGGAATTTTAATGTTAGGAGAGTGAGAAGTGATTTGAAAGCTTCTACTACGATGAGGAGTCGAGGATTGTGGTTGAATCGCGATAAGAGGATTGTTGGCGCGATTCCTGGGATTTGTATTGGTGATGTGTTTTTGTATAGGATGGAGTTGTGTGTGGTTGGTTTACATGGTCAACCACAAGCTGGGATTGATTATTTGCCGGGGAGTATGAGTTCGAATGGTGAACCGATTGCTACTAGTGTGATTGTTTCTGGTGGGTATGAGGATGATGTTGATGAAGGCGATGTTATTATTTACTCGGGTCATGGTGGACAGGATAAGAATTCGAGGCAGGTTTTTCATCAGAAGCTGGAAGGTGGGAATCTTGCTATGGAGAGGAGTATGCATTATGGGATTGAGGTTAGGGTTATTCGCGGTGTGAGGTATGAAGGTACTTCTTCGACTTCGGGTAAGGTTTATGTTTATGATGGTTTGTATAGGATTATTGAATGCTGGTTTGATGTTGGTAAAAGTGGTTTTGGTGTTTTTAAGTTTAAGTTATGGAGGATTGATGGTCAAGCTAAGATGGGTAGTTTGATTTTAAAGGAGGCTTTTTTGCTTAGGAGAGATCCTTTGTGTTATAAACCTATGTGTGTTATATCTCTTGATATTTCCAAGGGTATGGAGCGTGTCGGTATAAGGCTTTTTAATGATATTGATAAATGTAATGATCCGATGTGTTTTGAGTATCTTCCTAGGGCTACTTTTCCACTCTTTGTGTTTCATGAAAGTGGGAATGCGACGGGTTGTCAATGTGAGAGCTTTTGTGGGGAAGGATGTTTTTGTTTCATAAAAAATGGAAATGACTTTCCTTATAGTCAAAGCGGGCTTCTCTTGAAAGGAAAGCCGGTCATTTTTGAATGTGGTCCTTCTTGTAGTTGTCCGCCTCATTGTCGGAACCGTGTTACGCAAAAAGGGCTTAAACATAGGTTGGAAGTGTTTAGATCTAGGGAGACTGGTTGGGGAGTTAGGTCATTGGACCTTATTCAAGCTGGTGCATTTATATGCGAGTACACTGGGGTTGTTCTGACAAGGGAGCAATCAGAAATTATGACCATGAGTGGTGATTCATTAATATATCCGAATCGTTTTTCAAACAGGTGGACGGAATGGGGTAATTTATCTCTGATTCAAGATGGTTATGTGCCTCCTTCGTATCCATCAATTCCTCCTTTGGATTTTTCTCTTGATGTATCCAGAATGAGGAATGTGGCTTGCTATATAAGTCATAGCTCAACTCCTAATGTGATGGTTCAGTTTGTTTTGTATGATCATAATAATTTGATGTTCCCTCACATTATGCTGTTTGCGATGGAGAACATCCCTCCATTGAGAGAGTTCAGCCTTGATTATGGTGTAGCTGATGATGAGTTGACTGGCAAGCTCGTAATATGCAACTGA
Protein sequence

>MS.gene026348.t1

MNPHNPNQTFPSAPPGIPFPMLTPKQEPRDEPTQEFQYPNLENLPNQNLNLNLSLNLDFVSQGLEQPTTTTGAGVGVDAAKMAEIFRRSFTEGLQRQIQNNDAVDENPDANADARAIVPVSAGENNYNNAPPPAGEVMNVRKHKELVRMTDVGLPDQRQFRDVVRRTRMVYDSVRVLAMAEEEGNFNVRRVRSDLKASTTMRSRGLWLNRDKRIVGAIPGICIGDVFLYRMELCVVGLHGQPQAGIDYLPGSMSSNGEPIATSVIVSGGYEDDVDEGDVIIYSGHGGQDKNSRQVFHQKLEGGNLAMERSMHYGIEVRVIRGVRYEGTSSTSGKVYVYDGLYRIIECWFDVGKSGFGVFKFKLWRIDGQAKMGSLILKEAFLLRRDPLCYKPMCVISLDISKGMERVGIRLFNDIDKCNDPMCFEYLPRATFPLFVFHESGNATGCQCESFCGEGCFCFIKNGNDFPYSQSGLLLKGKPVIFECGPSCSCPPHCRNRVTQKGLKHRLEVFRSRETGWGVRSLDLIQAGAFICEYTGVVLTREQSEIMTMSGDSLIYPNRFSNRWTEWGNLSLIQDGYVPPSYPSIPPLDFSLDVSRMRNVACYISHSSTPNVMVQFVLYDHNNLMFPHIMLFAMENIPPLREFSLDYGVADDELTGKLVICN