AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene026358

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026358.t1 MTR_0223s0040 96.517 201 7 0 1 201 1 201 7.74e-146 407
MS.gene026358.t1 MTR_1g112370 62.941 170 49 6 2 158 3 171 7.86e-61 193
MS.gene026358.t1 MTR_2g023580 78.788 66 14 0 9 74 46 111 8.86e-33 118
MS.gene026358.t1 MTR_2g084230 69.737 76 23 0 5 80 84 159 1.03e-31 117
MS.gene026358.t1 MTR_2g084230 69.737 76 23 0 5 80 84 159 1.47e-31 118
MS.gene026358.t1 MTR_8g086410 68.000 75 24 0 6 80 97 171 1.08e-30 116
MS.gene026358.t1 MTR_8g086410 68.000 75 24 0 6 80 99 173 1.08e-30 116
MS.gene026358.t1 MTR_2g016220 64.286 84 25 1 6 84 77 160 5.60e-30 114
MS.gene026358.t1 MTR_2g016220 64.286 84 25 1 6 84 77 160 5.65e-30 114
MS.gene026358.t1 MTR_7g069660 65.278 72 25 0 3 74 56 127 1.03e-28 110
MS.gene026358.t1 MTR_8g086410 62.195 82 24 1 6 80 99 180 3.94e-28 109
MS.gene026358.t1 MTR_2g016220 61.364 88 25 2 6 84 77 164 8.39e-28 108
MS.gene026358.t1 MTR_3g450310 63.636 66 24 0 10 75 11 76 3.42e-25 97.8
MS.gene026358.t1 MTR_6g071625 64.407 59 21 0 17 75 201 259 2.93e-22 92.8
MS.gene026358.t1 MTR_8g066310 67.241 58 19 0 17 74 172 229 4.67e-22 92.0
MS.gene026358.t1 MTR_5g029470 49.000 100 43 2 17 111 218 314 2.44e-21 90.9
MS.gene026358.t1 MTR_3g064840 65.517 58 20 0 17 74 273 330 5.24e-21 90.1
MS.gene026358.t1 MTR_1g021520 59.701 67 26 1 9 74 116 182 6.71e-21 88.2
MS.gene026358.t1 MTR_1g080330 69.643 56 17 0 19 74 176 231 6.72e-21 88.2
MS.gene026358.t1 MTR_1g080330 69.643 56 17 0 19 74 176 231 6.80e-21 89.4
MS.gene026358.t1 MTR_7g115530 56.579 76 31 1 19 94 219 292 1.19e-20 87.8
MS.gene026358.t1 MTR_7g115530 56.579 76 31 1 19 94 219 292 2.26e-20 87.4
MS.gene026358.t1 MTR_7g115530 67.857 56 18 0 19 74 219 274 2.48e-20 88.2
MS.gene026358.t1 MTR_7g098250 67.857 56 18 0 19 74 263 318 2.62e-20 88.2
MS.gene026358.t1 MTR_7g115530 67.857 56 18 0 19 74 219 274 7.63e-20 86.3
MS.gene026358.t1 MTR_7g115530 67.308 52 17 0 19 70 219 270 3.14e-18 80.9
MS.gene026358.t1 MTR_5g027440 53.247 77 30 1 1 71 2 78 4.15e-18 80.5
MS.gene026358.t1 MTR_7g115530 70.000 50 15 0 19 68 219 268 4.18e-18 80.5
MS.gene026358.t1 MTR_5g041350 51.316 76 34 1 19 94 16 88 8.46e-18 80.5
MS.gene026358.t1 MTR_6g032990 60.000 60 21 1 19 75 43 102 2.19e-17 79.7
MS.gene026358.t1 MTR_5g060610 58.824 68 13 2 1 67 1 54 5.38e-17 72.0
MS.gene026358.t1 MTR_6g444980 39.062 128 58 3 19 146 36 143 1.26e-16 77.4
MS.gene026358.t1 MTR_6g444980 39.062 128 58 3 19 146 36 143 1.49e-16 77.0
MS.gene026358.t1 MTR_7g093030 55.738 61 27 0 14 74 208 268 1.95e-16 75.9
MS.gene026358.t1 MTR_2g086450 60.377 53 21 0 19 71 46 98 1.95e-16 77.0
MS.gene026358.t1 MTR_2g027800 52.055 73 29 1 19 85 40 112 2.66e-16 75.9
MS.gene026358.t1 MTR_7g093030 55.738 61 27 0 14 74 208 268 2.74e-16 76.3
MS.gene026358.t1 MTR_1g090670 62.264 53 20 0 19 71 45 97 1.52e-15 73.6
MS.gene026358.t1 MTR_1g053835 52.857 70 32 1 2 71 243 311 1.61e-15 74.3
MS.gene026358.t1 MTR_2g027800 62.264 53 20 0 19 71 40 92 2.14e-15 73.2
MS.gene026358.t1 MTR_2g027860 53.425 73 33 1 20 91 51 123 2.17e-15 73.6
MS.gene026358.t1 MTR_7g088070 67.308 52 17 0 20 71 52 103 2.87e-15 72.8
MS.gene026358.t1 MTR_7g088070 67.308 52 17 0 20 71 52 103 3.04e-15 72.4
MS.gene026358.t1 MTR_1g053830 38.298 94 51 1 1 87 1 94 6.36e-15 67.8
MS.gene026358.t1 MTR_4g081710 58.491 53 22 0 19 71 18 70 7.93e-15 72.0
MS.gene026358.t1 MTR_1g053800 60.377 53 21 0 20 72 179 231 1.83e-14 70.9
MS.gene026358.t1 MTR_4g021790 48.529 68 33 2 5 71 154 220 1.83e-13 67.4
MS.gene026358.t1 MTR_4g086835 63.265 49 18 0 23 71 279 327 2.28e-13 68.2
MS.gene026358.t1 MTR_4g113140 61.224 49 19 0 23 71 240 288 1.25e-12 65.9
MS.gene026358.t1 MTR_4g021855 43.529 85 43 3 5 85 154 237 5.30e-12 63.5
MS.gene026358.t1 MTR_4g086835 63.265 49 17 1 23 71 279 326 7.46e-12 63.5
MS.gene026358.t1 MTR_4g064730 49.231 65 32 1 7 71 120 183 1.43e-11 62.4
MS.gene026358.t1 MTR_7g068600 56.364 55 21 1 20 71 15 69 1.99e-11 61.6
MS.gene026358.t1 MTR_2g450070 50.909 55 26 1 17 71 212 265 2.53e-11 62.4
MS.gene026358.t1 MTR_7g093010 52.830 53 25 0 20 72 215 267 2.68e-11 62.0
MS.gene026358.t1 MTR_2g450070 50.909 55 26 1 17 71 212 265 2.75e-11 62.4
MS.gene026358.t1 MTR_7g089010 56.364 55 23 1 17 71 90 143 3.67e-11 61.2
MS.gene026358.t1 MTR_4g131600 53.030 66 30 1 17 82 200 264 5.14e-11 61.6
MS.gene026358.t1 MTR_4g121020 42.857 84 43 2 17 100 214 292 5.91e-11 61.2
MS.gene026358.t1 MTR_4g121020 42.857 84 43 2 17 100 214 292 6.68e-11 61.2
MS.gene026358.t1 MTR_4g121020 42.857 84 43 2 17 100 110 188 6.72e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026358.t1 AT1G14600 72.152 79 17 1 1 74 1 79 1.42e-32 118
MS.gene026358.t1 AT2G02060 82.258 62 11 0 10 71 21 82 2.44e-31 115
MS.gene026358.t1 AT2G02060 82.258 62 11 0 10 71 21 82 2.44e-31 115
MS.gene026358.t1 AT4G04605 68.493 73 23 0 9 81 5 77 4.86e-31 113
MS.gene026358.t1 AT2G40260 73.913 69 18 0 8 76 72 140 3.38e-29 112
MS.gene026358.t1 AT2G38300 69.014 71 22 0 10 80 46 116 1.25e-27 107
MS.gene026358.t1 AT2G42660 69.841 63 19 0 10 72 42 104 8.69e-27 103
MS.gene026358.t1 AT4G04555 53.333 75 35 0 6 80 2 76 3.36e-23 89.7
MS.gene026358.t1 AT1G32240 65.517 58 20 0 17 74 212 269 1.95e-21 90.5
MS.gene026358.t1 AT1G32240 65.517 58 20 0 17 74 212 269 2.08e-21 90.9
MS.gene026358.t1 AT5G16560 65.517 58 20 0 17 74 218 275 2.37e-21 90.9
MS.gene026358.t1 AT5G42630 62.069 58 22 0 17 74 104 161 3.66e-21 87.4
MS.gene026358.t1 AT4G17695 61.017 59 23 0 17 75 163 221 5.15e-21 89.0
MS.gene026358.t1 AT5G42630 62.069 58 22 0 17 74 104 161 5.63e-21 88.2
MS.gene026358.t1 AT4G04580 52.055 73 35 0 9 81 5 77 1.80e-20 84.3
MS.gene026358.t1 AT4G28610 55.263 76 33 1 20 95 227 301 4.18e-19 84.7
MS.gene026358.t1 AT5G45580 57.143 70 30 0 14 83 3 72 1.45e-18 81.3
MS.gene026358.t1 AT5G45580 57.143 70 30 0 14 83 19 88 1.62e-18 81.6
MS.gene026358.t1 AT3G04450 63.636 55 20 0 20 74 200 254 2.54e-18 82.4
MS.gene026358.t1 AT3G04450 63.636 55 20 0 20 74 200 254 2.54e-18 82.4
MS.gene026358.t1 AT3G04450 63.636 55 20 0 20 74 240 294 2.55e-18 81.6
MS.gene026358.t1 AT5G29000 63.636 55 20 0 20 74 190 244 2.94e-18 82.0
MS.gene026358.t1 AT5G29000 63.636 55 20 0 20 74 190 244 2.94e-18 82.0
MS.gene026358.t1 AT3G04450 63.636 55 20 0 20 74 240 294 2.96e-18 82.4
MS.gene026358.t1 AT5G29000 63.636 55 20 0 20 74 233 287 4.07e-18 81.6
MS.gene026358.t1 AT5G29000 63.636 55 20 0 20 74 233 287 4.18e-18 80.9
MS.gene026358.t1 AT4G13640 37.255 153 89 4 19 170 38 184 5.61e-18 80.1
MS.gene026358.t1 AT2G06020 43.478 92 46 2 13 98 81 172 7.76e-17 77.4
MS.gene026358.t1 AT3G24120 38.400 125 74 2 19 142 42 164 8.32e-17 77.0
MS.gene026358.t1 AT2G20400 63.636 55 20 0 20 74 233 287 8.76e-17 77.8
MS.gene026358.t1 AT2G20400 63.636 55 20 0 20 74 233 287 8.79e-17 77.8
MS.gene026358.t1 AT4G13640 42.500 120 66 3 19 137 38 155 1.15e-16 76.6
MS.gene026358.t1 AT3G13040 67.308 52 17 0 20 71 243 294 1.86e-16 77.0
MS.gene026358.t1 AT3G13040 67.308 52 17 0 20 71 243 294 1.86e-16 77.0
MS.gene026358.t1 AT3G13040 67.308 52 17 0 20 71 243 294 1.86e-16 77.0
MS.gene026358.t1 AT5G18240 49.296 71 33 1 19 86 46 116 2.38e-16 76.6
MS.gene026358.t1 AT5G18240 49.296 71 33 1 19 86 46 116 2.38e-16 76.6
MS.gene026358.t1 AT5G18240 49.296 71 33 1 19 86 46 116 2.61e-16 76.6
MS.gene026358.t1 AT5G18240 49.296 71 33 1 19 86 46 116 2.79e-16 76.6
MS.gene026358.t1 AT5G18240 49.296 71 33 1 19 86 46 116 2.79e-16 76.6
MS.gene026358.t1 AT3G12730 62.264 53 20 0 19 71 24 76 2.83e-16 74.7
MS.gene026358.t1 AT3G04030 56.604 53 23 0 19 71 46 98 6.29e-16 75.5
MS.gene026358.t1 AT3G04030 56.604 53 23 0 19 71 46 98 6.56e-16 75.5
MS.gene026358.t1 AT3G24120 39.167 120 70 2 19 137 42 159 8.57e-16 74.3
MS.gene026358.t1 AT1G79430 62.264 53 20 0 19 71 35 87 1.27e-15 74.3
MS.gene026358.t1 AT2G01060 67.308 52 17 0 20 71 17 68 1.84e-15 73.2
MS.gene026358.t1 AT1G69580 40.698 86 48 1 19 101 35 120 7.19e-15 72.0
MS.gene026358.t1 AT1G69580 40.698 86 48 1 19 101 35 120 7.82e-15 72.0
MS.gene026358.t1 AT5G06800 61.538 52 20 0 20 71 194 245 3.57e-14 69.7
MS.gene026358.t1 AT5G06800 61.538 52 20 0 20 71 194 245 6.19e-14 69.7
MS.gene026358.t1 AT5G06800 61.538 52 20 0 20 71 194 245 6.41e-14 69.7
MS.gene026358.t1 AT3G04030 52.830 53 24 1 19 71 46 97 4.45e-13 67.4
MS.gene026358.t1 AT4G37180 55.932 59 26 0 13 71 205 263 9.02e-13 66.2
MS.gene026358.t1 AT2G03500 61.224 49 19 0 23 71 238 286 1.08e-12 66.2
MS.gene026358.t1 AT4G37180 55.932 59 26 0 13 71 212 270 1.15e-12 65.9
MS.gene026358.t1 AT5G44190 56.897 58 24 1 17 74 146 202 1.57e-12 65.9
MS.gene026358.t1 AT5G44190 56.897 58 24 1 17 74 146 202 2.00e-12 65.1
MS.gene026358.t1 AT1G25550 59.184 49 20 0 23 71 214 262 3.03e-12 64.7
MS.gene026358.t1 AT5G59570 55.357 56 24 1 16 71 139 193 3.06e-12 64.3
MS.gene026358.t1 AT5G59570 55.357 56 24 1 16 71 139 193 3.06e-12 64.3
MS.gene026358.t1 AT3G62670 46.269 67 32 1 16 78 209 275 3.18e-12 64.7
MS.gene026358.t1 AT3G46640 54.545 55 24 1 17 71 143 196 1.35e-11 62.8
MS.gene026358.t1 AT3G46640 54.545 55 24 1 17 71 143 196 1.35e-11 62.8
MS.gene026358.t1 AT3G10760 56.364 55 23 1 17 71 104 157 1.37e-11 62.8
MS.gene026358.t1 AT5G05090 56.364 55 23 1 17 71 80 133 1.56e-11 62.0
MS.gene026358.t1 AT3G46640 54.545 55 24 1 17 71 143 196 1.86e-11 62.4
MS.gene026358.t1 AT4G37180 54.386 57 26 0 13 69 205 261 1.96e-11 62.0
MS.gene026358.t1 AT2G40970 56.364 55 23 1 17 71 104 157 4.00e-11 60.8
MS.gene026358.t1 AT1G25550 55.102 49 22 0 23 71 214 262 4.30e-11 60.8
MS.gene026358.t1 AT1G49190 46.875 64 31 1 11 71 208 271 7.91e-11 60.8

Find 38 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCAGTTGATGGATGTTAA+AGG 0.259007 1.4:+19659869 MS.gene026358:CDS
TAAGCTTGTTCTTCAGTTGA+TGG 0.287799 1.4:+19659859 MS.gene026358:CDS
GAACAAATCAGTTTCTTGTT+TGG 0.311474 1.4:-19661442 None:intergenic
AACAAATCAGTTTCTTGTTT+GGG 0.342359 1.4:-19661441 None:intergenic
ACAGAAATTTCTCCCCAAAA+AGG 0.367281 1.4:+19660620 MS.gene026358:CDS
ACATGTTAAGAGCCATTTGC+AGG 0.373744 1.4:+19659904 MS.gene026358:CDS
GTTTATGCCATTGAAACTCT+TGG 0.378805 1.4:+19659694 MS.gene026358:CDS
ATATTCTTTCTCTGCATTCA+AGG 0.392029 1.4:-19661269 None:intergenic
GCAACAGCAGAAGGGAATAA+AGG 0.396273 1.4:+19661350 MS.gene026358:CDS
TTTGTTGTGTTGGTTCAATA+TGG 0.396998 1.4:+19661376 MS.gene026358:CDS
TTCAGTTGATGGATGTTAAA+GGG 0.399431 1.4:+19659870 MS.gene026358:CDS
ACAAAGCAACGATGAAGTTC+AGG 0.401903 1.4:-19659673 None:intergenic
TTTAGAGATCATGAATCTCT+TGG 0.412921 1.4:+19660571 MS.gene026358:CDS
TTTGTTTAAACCAGATGTAT+AGG 0.415716 1.4:+19660010 MS.gene026358:intron
CAAAGGTTCCTCGTTTAAGA+TGG 0.432536 1.4:+19659647 MS.gene026358:CDS
ATGACCACCAAGAGTTTCAA+TGG 0.434104 1.4:-19659701 None:intergenic
ACAAGAAACTGATTTGTTCC+AGG 0.436372 1.4:+19661446 MS.gene026358:CDS
GGAAAGATTGTGAAATATTG+AGG 0.465565 1.4:-19661411 None:intergenic
GAGAAGAGAAATTCCCTCTC+AGG 0.479292 1.4:+19661243 MS.gene026358:CDS
TTCTGGAGCATGATGATGAT+GGG 0.488907 1.4:+19660548 MS.gene026358:CDS
TATCAAAATCAACCTGCAAA+TGG 0.495949 1.4:-19659916 None:intergenic
GTTTCTTGTTTGGGTAGCAC+AGG 0.498612 1.4:-19661432 None:intergenic
ATAAAGGAGATTTGTTGTGT+TGG 0.503985 1.4:+19661366 MS.gene026358:CDS
ATCAACTGAAGAACAAGCTT+AGG 0.508937 1.4:-19659857 None:intergenic
ATTGTGAAATATTGAGGTTG+AGG 0.511226 1.4:-19661405 None:intergenic
TTTCTGGAGCATGATGATGA+TGG 0.516346 1.4:+19660547 MS.gene026358:CDS
CATGGTTCAGCAACAGCAGA+AGG 0.534523 1.4:+19661341 MS.gene026358:CDS
ATGAGTAGTTGTGGAAGGGA+AGG 0.542254 1.4:+19659601 MS.gene026358:CDS
TTATGATTATGTGCAGGACA+TGG 0.561583 1.4:+19661323 MS.gene026358:CDS
AGATCATGAATCTCTTGGAA+TGG 0.569091 1.4:+19660576 MS.gene026358:CDS
GCATTCAAGGATTCCTGAGA+GGG 0.578921 1.4:-19661256 None:intergenic
GAGACAATATGTAAGATCAA+AGG 0.602756 1.4:+19659630 MS.gene026358:CDS
AGAGGTGACTCATGCAAACA+AGG 0.619444 1.4:+19660039 MS.gene026358:CDS
TATGCCATTGAAACTCTTGG+TGG 0.619663 1.4:+19659697 MS.gene026358:CDS
CAGATGTATAGGAGTATGAG+AGG 0.628713 1.4:+19660021 MS.gene026358:intron
TGCATTCAAGGATTCCTGAG+AGG 0.645375 1.4:-19661257 None:intergenic
ATGGTTCAGCAACAGCAGAA+GGG 0.660060 1.4:+19661342 MS.gene026358:CDS
TCAGGTGTCCATCTTAAACG+AGG 0.752182 1.4:-19659655 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAATTTGATTAACATT+TGG + chr1.4:19661190-19661209 MS.gene026358:intron 10.0%
!! ATTAAGATTATATAGTATAT+CGG + chr1.4:19660807-19660826 MS.gene026358:intron 10.0%
!! GAAAATTATAATATAAATGA+TGG - chr1.4:19660288-19660307 None:intergenic 10.0%
!!! ATTATTTTTAAACCATAAAA+TGG - chr1.4:19661173-19661192 None:intergenic 10.0%
!!! TTTTGTTTTGTTAATTTATT+AGG + chr1.4:19659977-19659996 MS.gene026358:intron 10.0%
!! ATAAAATGGAAAAGTAAAAA+TGG - chr1.4:19661159-19661178 None:intergenic 15.0%
!! GATATACTATATAATCTTAA+TGG - chr1.4:19660808-19660827 None:intergenic 15.0%
!!! AAAAGATTGTTTTTTATGTA+CGG - chr1.4:19660530-19660549 None:intergenic 15.0%
!!! AATAATTTCAAACTTTTCAA+AGG + chr1.4:19660671-19660690 MS.gene026358:intron 15.0%
!!! AATATTAAAGCTTTATACTA+AGG - chr1.4:19660086-19660105 None:intergenic 15.0%
!!! AGTTAACAAAACAATTTTTT+AGG + chr1.4:19661090-19661109 MS.gene026358:intron 15.0%
!!! ATCATAAGAAGATTTTAAAA+AGG - chr1.4:19660350-19660369 None:intergenic 15.0%
!!! ATTTTCTACGATATTAAAAA+TGG - chr1.4:19660938-19660957 None:intergenic 15.0%
!!! ATTTTTACTTTTCCATTTTA+TGG + chr1.4:19661158-19661177 MS.gene026358:intron 15.0%
!! ACATATTAAGGAAATTAGAT+TGG - chr1.4:19660476-19660495 None:intergenic 20.0%
!! ATATAAACCAACACATATTA+AGG - chr1.4:19660488-19660507 None:intergenic 20.0%
!! GAATTTATTGAAAAACTTGA+TGG + chr1.4:19661061-19661080 MS.gene026358:intron 20.0%
!! TAAAAAGTAGTGTATGTATT+TGG - chr1.4:19661306-19661325 None:intergenic 20.0%
!! TAAAGAAGAAAATAAAGCTA+TGG - chr1.4:19660649-19660668 None:intergenic 20.0%
!! TTATAACATGTTCACTATTA+CGG + chr1.4:19660957-19660976 MS.gene026358:intron 20.0%
!!! ACATAAAAAACAATCTTTTC+TGG + chr1.4:19660531-19660550 MS.gene026358:CDS 20.0%
!!! ATATTTCTTTTCATTTGTAG+AGG + chr1.4:19659829-19659848 MS.gene026358:intron 20.0%
!!! ATTTTTTAGGAAAAAGTGTT+TGG + chr1.4:19661103-19661122 MS.gene026358:intron 20.0%
! CTAATTTCCTTAATATGTGT+TGG + chr1.4:19660478-19660497 MS.gene026358:intron 25.0%
! TGTTTCAAATAGCATTAACA+AGG - chr1.4:19660445-19660464 None:intergenic 25.0%
! TTGGTTTATATTTGACAGAT+AGG + chr1.4:19660497-19660516 MS.gene026358:intron 25.0%
! TTTGTTTAAACCAGATGTAT+AGG + chr1.4:19660010-19660029 MS.gene026358:intron 25.0%
!! AACAAATCAGTTTCTTGTTT+GGG - chr1.4:19661444-19661463 None:intergenic 25.0%
!! TTAAAGCTTTATACTAAGGA+AGG - chr1.4:19660082-19660101 None:intergenic 25.0%
!!! TACTTTTTATGATTATGTGC+AGG + chr1.4:19661317-19661336 MS.gene026358:CDS 25.0%
ATATTCTTTCTCTGCATTCA+AGG - chr1.4:19661272-19661291 None:intergenic 30.0%
ATTGTGAAATATTGAGGTTG+AGG - chr1.4:19661408-19661427 None:intergenic 30.0%
GAAAACTACATTAGTGATTG+CGG - chr1.4:19660897-19660916 None:intergenic 30.0%
GAACAAATCAGTTTCTTGTT+TGG - chr1.4:19661445-19661464 None:intergenic 30.0%
GAGACAATATGTAAGATCAA+AGG + chr1.4:19659630-19659649 MS.gene026358:CDS 30.0%
GGAAAGATTGTGAAATATTG+AGG - chr1.4:19661414-19661433 None:intergenic 30.0%
TATCAAAATCAACCTGCAAA+TGG - chr1.4:19659919-19659938 None:intergenic 30.0%
TTTAGAGATCATGAATCTCT+TGG + chr1.4:19660571-19660590 MS.gene026358:CDS 30.0%
! AGATTTTCCAAATTTGTGCA+TGG - chr1.4:19660254-19660273 None:intergenic 30.0%
! CTTTTCAAAGGACTTAAAAG+TGG + chr1.4:19660683-19660702 MS.gene026358:intron 30.0%
! TTGGACTTTTGCTTGATTTT+AGG + chr1.4:19661122-19661141 MS.gene026358:intron 30.0%
!! ATAAAGGAGATTTGTTGTGT+TGG + chr1.4:19661366-19661385 MS.gene026358:CDS 30.0%
!! TTCAGTTGATGGATGTTAAA+GGG + chr1.4:19659870-19659889 MS.gene026358:CDS 30.0%
!! TTTGTTGTGTTGGTTCAATA+TGG + chr1.4:19661376-19661395 MS.gene026358:CDS 30.0%
ACACAATACAAGTACTACAC+CGG - chr1.4:19660736-19660755 None:intergenic 35.0%
ACAGAAATTTCTCCCCAAAA+AGG + chr1.4:19660620-19660639 MS.gene026358:CDS 35.0%
AGATCATGAATCTCTTGGAA+TGG + chr1.4:19660576-19660595 MS.gene026358:CDS 35.0%
AGGACTTAAAAGTGGTATAG+TGG + chr1.4:19660691-19660710 MS.gene026358:intron 35.0%
ATCAACTGAAGAACAAGCTT+AGG - chr1.4:19659860-19659879 None:intergenic 35.0%
ATGCTTTATGCATTGAAGCT+AGG + chr1.4:19660175-19660194 MS.gene026358:intron 35.0%
CTAGTTTCCATGCACAAATT+TGG + chr1.4:19660244-19660263 MS.gene026358:intron 35.0%
GACTTAAAAGTGGTATAGTG+GGG + chr1.4:19660693-19660712 MS.gene026358:intron 35.0%
GGACTTAAAAGTGGTATAGT+GGG + chr1.4:19660692-19660711 MS.gene026358:intron 35.0%
GTGTATGTTTGATATCAGAG+TGG - chr1.4:19661007-19661026 None:intergenic 35.0%
GTTTATGCCATTGAAACTCT+TGG + chr1.4:19659694-19659713 MS.gene026358:CDS 35.0%
TAACATGTTCACTATTACGG+TGG + chr1.4:19660960-19660979 MS.gene026358:intron 35.0%
TGTTGTTATGACATTAGTCG+CGG + chr1.4:19661036-19661055 MS.gene026358:intron 35.0%
TTATGATTATGTGCAGGACA+TGG + chr1.4:19661323-19661342 MS.gene026358:CDS 35.0%
TTTGATATCAGAGTGGTTCA+TGG - chr1.4:19661000-19661019 None:intergenic 35.0%
! AAAGCTATGGTGACCTTTTT+GGG - chr1.4:19660636-19660655 None:intergenic 35.0%
! TAAAGCTATGGTGACCTTTT+TGG - chr1.4:19660637-19660656 None:intergenic 35.0%
! TAAGCTTGTTCTTCAGTTGA+TGG + chr1.4:19659859-19659878 MS.gene026358:CDS 35.0%
!! CTTCAGTTGATGGATGTTAA+AGG + chr1.4:19659869-19659888 MS.gene026358:CDS 35.0%
AAGGAAGGTGAGACATTGTT+AGG - chr1.4:19660067-19660086 None:intergenic 40.0%
ACAAAGCAACGATGAAGTTC+AGG - chr1.4:19659676-19659695 None:intergenic 40.0%
ACATGTTAAGAGCCATTTGC+AGG + chr1.4:19659904-19659923 MS.gene026358:CDS 40.0%
ATGACCACCAAGAGTTTCAA+TGG - chr1.4:19659704-19659723 None:intergenic 40.0%
CAAAGGTTCCTCGTTTAAGA+TGG + chr1.4:19659647-19659666 MS.gene026358:CDS 40.0%
CACAATACAAGTACTACACC+GGG - chr1.4:19660735-19660754 None:intergenic 40.0%
CTCTCATACTCCTATACATC+TGG - chr1.4:19660023-19660042 None:intergenic 40.0%
GTGCATGGAAACTAGATCAT+AGG - chr1.4:19660239-19660258 None:intergenic 40.0%
TATGCCATTGAAACTCTTGG+TGG + chr1.4:19659697-19659716 MS.gene026358:CDS 40.0%
! AAGCTATGGTGACCTTTTTG+GGG - chr1.4:19660635-19660654 None:intergenic 40.0%
! CAGATGTATAGGAGTATGAG+AGG + chr1.4:19660021-19660040 MS.gene026358:intron 40.0%
! TGCGTCTTACATGACTTTTG+TGG + chr1.4:19660200-19660219 MS.gene026358:intron 40.0%
!! CTAATATGACTAGCTTCGTG+TGG - chr1.4:19660777-19660796 None:intergenic 40.0%
!! TTCTGGAGCATGATGATGAT+GGG + chr1.4:19660548-19660567 MS.gene026358:CDS 40.0%
!! TTTCTGGAGCATGATGATGA+TGG + chr1.4:19660547-19660566 MS.gene026358:CDS 40.0%
AGAGGTGACTCATGCAAACA+AGG + chr1.4:19660039-19660058 MS.gene026358:CDS 45.0%
ATGAGTAGTTGTGGAAGGGA+AGG + chr1.4:19659601-19659620 MS.gene026358:CDS 45.0%
ATGGTTCAGCAACAGCAGAA+GGG + chr1.4:19661342-19661361 MS.gene026358:CDS 45.0%
GAGAAGAGAAATTCCCTCTC+AGG + chr1.4:19661243-19661262 MS.gene026358:CDS 45.0%
GCAACAGCAGAAGGGAATAA+AGG + chr1.4:19661350-19661369 MS.gene026358:CDS 45.0%
GCATTCAAGGATTCCTGAGA+GGG - chr1.4:19661259-19661278 None:intergenic 45.0%
TCAGGTGTCCATCTTAAACG+AGG - chr1.4:19659658-19659677 None:intergenic 45.0%
TGCATTCAAGGATTCCTGAG+AGG - chr1.4:19661260-19661279 None:intergenic 45.0%
! AAAAGTGGTATAGTGGGGCA+TGG + chr1.4:19660698-19660717 MS.gene026358:intron 45.0%
! GGCATGGTTTAAAATCGTCC+CGG + chr1.4:19660714-19660733 MS.gene026358:intron 45.0%
! GTTTCTTGTTTGGGTAGCAC+AGG - chr1.4:19661435-19661454 None:intergenic 45.0%
CATGGTTCAGCAACAGCAGA+AGG + chr1.4:19661341-19661360 MS.gene026358:CDS 50.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 19659601 19661467 19659601 ID=MS.gene026358
chr1.4 mRNA 19659601 19661467 19659601 ID=MS.gene026358.t1;Parent=MS.gene026358
chr1.4 exon 19659601 19659727 19659601 ID=MS.gene026358.t1.exon1;Parent=MS.gene026358.t1
chr1.4 CDS 19659601 19659727 19659601 ID=cds.MS.gene026358.t1;Parent=MS.gene026358.t1
chr1.4 exon 19659849 19659925 19659849 ID=MS.gene026358.t1.exon2;Parent=MS.gene026358.t1
chr1.4 CDS 19659849 19659925 19659849 ID=cds.MS.gene026358.t1;Parent=MS.gene026358.t1
chr1.4 exon 19660024 19660060 19660024 ID=MS.gene026358.t1.exon3;Parent=MS.gene026358.t1
chr1.4 CDS 19660024 19660060 19660024 ID=cds.MS.gene026358.t1;Parent=MS.gene026358.t1
chr1.4 exon 19660515 19660641 19660515 ID=MS.gene026358.t1.exon4;Parent=MS.gene026358.t1
chr1.4 CDS 19660515 19660641 19660515 ID=cds.MS.gene026358.t1;Parent=MS.gene026358.t1
chr1.4 exon 19661233 19661467 19661233 ID=MS.gene026358.t1.exon5;Parent=MS.gene026358.t1
chr1.4 CDS 19661233 19661467 19661233 ID=cds.MS.gene026358.t1;Parent=MS.gene026358.t1
Gene Sequence

>MS.gene026358

ATGAGTAGTTGTGGAAGGGAAGGTGTTGTGAGACAATATGTAAGATCAAAGGTTCCTCGTTTAAGATGGACACCTGAACTTCATCGTTGCTTTGTTTATGCCATTGAAACTCTTGGTGGTCATCAAAGTATGTGTATTTCTACATTACTTTATTAGATGTTATTTTCTTCATTCATTTCTAGCACTATAGTTAATTTTTAATGAAAAAATTGTCGAAATATATTTATGATATTTCTTTTCATTTGTAGAGGCTACTCCTAAGCTTGTTCTTCAGTTGATGGATGTTAAAGGGTTAACAATTTCACATGTTAAGAGCCATTTGCAGGTTGATTTTGATATGCTTTCAAAATTATATTTTTGTCTATTTGTTTTTTTTTTTTGTTTTGTTAATTTATTAGGTTTCTCTTGTTTTGTTTAAACCAGATGTATAGGAGTATGAGAGGTGACTCATGCAAACAAGGTACCTAACAATGTCTCACCTTCCTTAGTATAAAGCTTTAATATTTATTATTCTTTAGATCATTGTTTTGTATAAAATATTTTTCATTAGAATGTCATGATACATTGATGCATGATGCTTTATGCATTGAAGCTAGGCATGCGTCTTACATGACTTTTGTGGTTAAAAATCAAAGCCTATGATCTAGTTTCCATGCACAAATTTGGAAAATCTGCAGCTATATACCATCATTTATATTATAATTTTCTTATATATGTATTGATGTTAGAATAATTATTTAAAGTGTCCTTTTTAAAATCTTCTTATGATGATATTTTGATGAAACATTTTAGTACTATCTAATATGAATTCATTTATAGTTTATGTTACTTCAAATCAAAACCTTGTTAATGCTATTTGAAACATGTACTTACCAATCTAATTTCCTTAATATGTGTTGGTTTATATTTGACAGATAGGACTACTTCCGTACATAAAAAACAATCTTTTCTGGAGCATGATGATGATGGGTTTAGAGATCATGAATCTCTTGGAATGGAATCTGATTCTCTCTTTAGCTACAGAAATTTCTCCCCAAAAAGGTCACCATAGCTTTATTTTCTTCTTTAGAAATAATTTCAAACTTTTCAAAGGACTTAAAAGTGGTATAGTGGGGCATGGTTTAAAATCGTCCCGGTGTAGTACTTGTATTGTGTGCGATCACAATATCGTGACCACACGAAGCTAGTCATATTAGTGATTGTTCCATTAAGATTATATAGTATATCGGTCTATTTTTATATTAAATTAAAGTACACACTTAAAATTGCAATAGTCATATCTTCATTGAATGACCGCAATCACTAATGTAGTTTTCTACACGCAGAACACAGATCCATTTTTAATATCGTAGAAAATTATAACATGTTCACTATTACGGTGGCTTTTTCGAAATCACCATGAACCACTCTGATATCAAACATACACTTAATCAATTGTTGTTATGACATTAGTCGCGGTCGAATTTATTGAAAAACTTGATGGTGAAAAAGTTAACAAAACAATTTTTTAGGAAAAAGTGTTTGGACTTTTGCTTGATTTTAGGCTGTTCTGAATCCATTTTTACTTTTCCATTTTATGGTTTAAAAATAATATAATTTGATTAACATTTGGATTCTCTTGATATATATCAGAGCTAGAATCGAGAAGAGAAATTCCCTCTCAGGAATCCTTGAATGCAGAGAAAGAATATGTGAAGAAGTTCCAAATACATACACTACTTTTTATGATTATGTGCAGGACATGGTTCAGCAACAGCAGAAGGGAATAAAGGAGATTTGTTGTGTTGGTTCAATATGGCATACTCCTCAACCTCAATATTTCACAATCTTTCCTGTGCTACCCAAACAAGAAACTGATTTGTTCCAG
Protein sequence

>MS.gene026358.t1

MSSCGREGVVRQYVRSKVPRLRWTPELHRCFVYAIETLGGHQKATPKLVLQLMDVKGLTISHVKSHLQMYRSMRGDSCKQDRTTSVHKKQSFLEHDDDGFRDHESLGMESDSLFSYRNFSPKRARIEKRNSLSGILECRERICEEVPNTYTTFYDYVQDMVQQQQKGIKEICCVGSIWHTPQPQYFTIFPVLPKQETDLFQ