AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene02641

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02641.t1 MTR_3g089055 94.690 565 30 0 1 565 6 570 0.0 1080
MS.gene02641.t1 MTR_3g089055 94.690 565 30 0 1 565 1 565 0.0 1077
MS.gene02641.t1 MTR_1g029420 69.950 599 139 7 1 565 1 592 0.0 794
MS.gene02641.t1 MTR_5g097480 50.962 416 198 1 156 565 129 544 8.08e-153 449
MS.gene02641.t1 MTR_2g082090 54.324 370 169 0 196 565 210 579 3.88e-145 431
MS.gene02641.t1 MTR_3g056110 49.606 381 191 1 186 565 162 542 2.12e-139 415
MS.gene02641.t1 MTR_5g094450 51.036 386 184 3 185 565 147 532 1.64e-137 410
MS.gene02641.t1 MTR_4g133660 47.568 370 193 1 196 564 173 542 3.15e-126 381
MS.gene02641.t1 MTR_2g026250 47.170 371 195 1 196 565 226 596 1.76e-122 373
MS.gene02641.t1 MTR_7g057230 38.767 454 255 10 119 564 218 656 9.70e-87 282
MS.gene02641.t1 MTR_6g047750 40.409 391 191 10 196 565 293 662 2.33e-80 265
MS.gene02641.t1 MTR_7g027190 37.726 387 211 5 197 565 299 673 1.38e-76 256
MS.gene02641.t1 MTR_3g065980 34.987 383 218 8 199 565 173 540 7.46e-67 226
MS.gene02641.t1 MTR_8g442410 34.908 381 223 7 199 565 156 525 8.58e-65 221
MS.gene02641.t1 MTR_4g064160 31.892 370 251 1 196 564 314 683 5.74e-63 219
MS.gene02641.t1 MTR_7g074650 36.339 366 218 6 201 565 441 792 4.96e-62 219
MS.gene02641.t1 MTR_4g104020 32.723 382 235 5 197 565 148 520 5.88e-61 210
MS.gene02641.t1 MTR_7g062120 30.479 397 269 2 176 565 344 740 2.47e-59 210
MS.gene02641.t1 MTR_4g064200 31.707 369 247 4 198 564 284 649 9.97e-58 204
MS.gene02641.t1 MTR_2g097310 30.376 372 252 3 196 564 270 637 4.13e-56 199
MS.gene02641.t1 MTR_2g097350 31.818 374 244 6 196 564 272 639 4.35e-56 199
MS.gene02641.t1 MTR_4g076140 28.791 455 253 8 171 565 26 469 4.85e-56 196
MS.gene02641.t1 MTR_2g097390 30.108 372 253 3 196 564 273 640 1.41e-55 198
MS.gene02641.t1 MTR_4g097080 30.423 378 250 6 196 565 131 503 1.87e-55 195
MS.gene02641.t1 MTR_4g064150 29.144 374 260 3 196 564 359 732 5.10e-55 198
MS.gene02641.t1 MTR_4g064120 28.054 442 303 5 130 562 188 623 1.52e-54 195
MS.gene02641.t1 MTR_4g102790 35.602 382 219 14 198 564 292 661 2.09e-54 196
MS.gene02641.t1 MTR_2g097410 30.585 376 250 7 196 564 369 740 4.43e-54 196
MS.gene02641.t1 MTR_4g064180 28.217 443 303 5 130 563 188 624 4.94e-54 194
MS.gene02641.t1 MTR_5g009080 28.605 430 240 7 197 565 55 478 5.26e-54 191
MS.gene02641.t1 MTR_3g022830 28.504 421 245 5 195 565 22 436 1.26e-51 183
MS.gene02641.t1 MTR_4g077760 32.181 376 232 8 199 565 194 555 1.27e-50 183
MS.gene02641.t1 MTR_2g097467 28.385 384 269 4 182 564 275 653 2.15e-50 184
MS.gene02641.t1 MTR_5g015490 31.486 397 247 10 185 565 103 490 4.91e-50 180
MS.gene02641.t1 MTR_4g076020 33.154 371 214 10 201 565 87 429 3.37e-49 176
MS.gene02641.t1 MTR_2g097463 28.804 368 258 3 198 564 290 654 4.75e-49 180
MS.gene02641.t1 MTR_2g097473 27.097 465 311 9 117 564 200 653 1.74e-48 179
MS.gene02641.t1 MTR_3g072710 31.863 408 237 12 185 564 105 499 8.64e-48 174
MS.gene02641.t1 MTR_1g096030 29.471 397 236 12 197 565 79 459 1.20e-45 167
MS.gene02641.t1 MTR_1g069725 32.181 376 224 11 198 564 109 462 1.02e-44 165
MS.gene02641.t1 MTR_5g015950 27.962 422 242 8 196 564 33 445 3.41e-43 160
MS.gene02641.t1 MTR_3g053270 27.962 422 242 8 196 564 33 445 3.41e-43 160
MS.gene02641.t1 MTR_2g097380 27.701 361 229 4 205 564 231 560 1.26e-41 158
MS.gene02641.t1 MTR_2g099110 29.049 389 246 8 197 565 73 451 4.02e-41 154
MS.gene02641.t1 MTR_2g089100 26.018 442 279 8 156 564 16 442 2.62e-40 152
MS.gene02641.t1 MTR_4g095500 26.835 436 273 7 174 564 33 467 1.04e-38 148
MS.gene02641.t1 MTR_5g058860 30.548 383 247 10 197 564 118 496 1.69e-38 148
MS.gene02641.t1 MTR_2g034250 26.098 410 263 11 178 565 194 585 2.63e-30 125
MS.gene02641.t1 MTR_0092s0100 27.957 372 247 8 199 565 376 731 2.00e-28 120
MS.gene02641.t1 MTR_0092s0100 27.957 372 247 8 199 565 373 728 2.13e-28 120
MS.gene02641.t1 MTR_2g034260 26.555 418 250 14 178 565 194 584 4.73e-28 119
MS.gene02641.t1 MTR_7g109580 26.649 379 256 10 197 565 188 554 8.82e-27 114
MS.gene02641.t1 MTR_2g034280 25.455 385 256 10 199 565 204 575 1.15e-26 114
MS.gene02641.t1 MTR_8g093070 27.441 379 241 12 198 564 139 495 6.55e-25 108
MS.gene02641.t1 MTR_4g026485 25.995 377 247 11 199 565 270 624 1.15e-23 105
MS.gene02641.t1 MTR_1g086970 27.875 287 185 9 287 565 206 478 6.31e-20 93.2
MS.gene02641.t1 MTR_8g020840 23.632 402 265 15 190 565 163 548 6.77e-20 93.6
MS.gene02641.t1 MTR_3g022580 30.864 162 106 2 404 565 28 183 5.39e-18 82.4
MS.gene02641.t1 MTR_3g022005 37.634 93 58 0 473 565 33 125 1.88e-13 67.8
MS.gene02641.t1 MTR_8g077940 21.891 402 236 14 199 565 183 541 8.72e-13 71.2
MS.gene02641.t1 MTR_4g074310 29.237 236 121 7 201 431 2 196 1.07e-12 68.2
MS.gene02641.t1 MTR_4g074320 32.558 172 89 6 171 337 39 188 5.73e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02641.t1 AT1G21450 59.496 595 194 16 1 565 16 593 0.0 644
MS.gene02641.t1 AT5G48150 52.911 395 183 2 172 565 98 490 2.66e-146 431
MS.gene02641.t1 AT5G48150 52.911 395 183 2 172 565 98 490 2.66e-146 431
MS.gene02641.t1 AT5G48150 52.911 395 183 2 172 565 98 490 2.66e-146 431
MS.gene02641.t1 AT5G48150 52.911 395 183 2 172 565 98 490 2.66e-146 431
MS.gene02641.t1 AT1G50600 51.948 385 184 1 182 565 142 526 6.95e-142 421
MS.gene02641.t1 AT1G50600 51.948 385 184 1 182 565 213 597 5.78e-141 421
MS.gene02641.t1 AT1G50600 51.948 385 184 1 182 565 217 601 9.64e-141 421
MS.gene02641.t1 AT2G04890 51.892 370 171 1 196 565 51 413 4.50e-135 399
MS.gene02641.t1 AT4G17230 47.027 370 196 0 196 565 156 525 3.07e-124 375
MS.gene02641.t1 AT4G17230 47.027 370 196 0 196 565 156 525 3.07e-124 375
MS.gene02641.t1 AT5G52510 39.481 385 208 5 196 565 266 640 3.99e-85 277
MS.gene02641.t1 AT1G66350 37.097 372 208 9 199 565 156 506 2.79e-69 232
MS.gene02641.t1 AT3G03450 36.340 377 215 9 199 565 184 545 8.78e-68 229
MS.gene02641.t1 AT5G17490 35.242 454 246 13 136 565 87 516 2.44e-67 227
MS.gene02641.t1 AT3G54220 36.856 369 213 8 201 564 296 649 4.36e-66 227
MS.gene02641.t1 AT1G07530 33.760 391 254 3 176 564 377 764 2.17e-64 224
MS.gene02641.t1 AT5G66770 36.340 377 217 9 199 565 221 584 1.95e-60 210
MS.gene02641.t1 AT1G14920 33.780 373 225 10 199 565 173 529 1.65e-59 207
MS.gene02641.t1 AT2G01570 34.301 379 215 12 199 565 225 581 2.92e-58 204
MS.gene02641.t1 AT2G29060 31.980 394 252 5 179 564 269 654 2.05e-57 204
MS.gene02641.t1 AT2G29060 31.980 394 252 5 179 564 306 691 2.94e-57 204
MS.gene02641.t1 AT2G37650 29.919 371 257 2 196 565 333 701 1.74e-56 202
MS.gene02641.t1 AT2G37650 29.919 371 257 2 196 565 345 713 1.90e-56 202
MS.gene02641.t1 AT5G59450 29.777 403 271 5 172 565 199 598 5.31e-56 199
MS.gene02641.t1 AT2G29065 30.423 378 254 5 196 564 250 627 9.66e-54 193
MS.gene02641.t1 AT3G46600 29.919 371 258 2 196 565 2 371 1.14e-53 187
MS.gene02641.t1 AT3G46600 29.919 371 258 2 196 565 210 579 1.91e-52 189
MS.gene02641.t1 AT3G46600 29.919 371 258 2 196 565 178 547 2.03e-52 188
MS.gene02641.t1 AT5G41920 32.718 379 223 10 198 565 43 400 1.92e-51 182
MS.gene02641.t1 AT3G50650 33.983 359 209 7 221 565 198 542 1.77e-50 182
MS.gene02641.t1 AT1G07520 27.820 399 272 5 182 564 130 528 6.97e-50 181
MS.gene02641.t1 AT1G07520 27.820 399 272 5 182 564 130 528 6.97e-50 181
MS.gene02641.t1 AT1G07520 27.820 399 272 5 182 564 294 692 1.30e-49 182
MS.gene02641.t1 AT1G07520 27.820 399 272 5 182 564 295 693 1.30e-49 182
MS.gene02641.t1 AT4G37650 29.206 428 259 11 165 565 119 529 1.57e-48 177
MS.gene02641.t1 AT1G63100 31.362 389 233 8 198 564 252 628 1.43e-43 164
MS.gene02641.t1 AT1G63100 33.141 347 212 7 198 536 277 611 1.62e-43 165
MS.gene02641.t1 AT4G08250 29.825 342 220 7 233 564 145 476 4.90e-42 158
MS.gene02641.t1 AT1G55580 27.119 413 249 9 196 565 42 445 1.96e-40 152
MS.gene02641.t1 AT3G49950 27.720 386 256 9 196 564 28 407 8.45e-39 147
MS.gene02641.t1 AT1G50420 32.857 210 132 2 197 403 56 259 1.16e-30 125
MS.gene02641.t1 AT1G50420 37.748 151 91 2 417 565 330 479 9.45e-28 117
MS.gene02641.t1 AT3G13840 26.000 400 267 11 178 565 128 510 4.58e-22 100
MS.gene02641.t1 AT3G60630 25.501 349 229 10 231 565 291 622 3.72e-21 97.8
MS.gene02641.t1 AT2G45160 26.214 309 202 8 268 565 346 639 5.70e-21 97.4
MS.gene02641.t1 AT4G00150 24.069 349 234 10 222 565 232 554 9.94e-19 90.1
MS.gene02641.t1 AT5G67411 33.668 199 119 6 315 502 2 198 5.98e-18 82.8
MS.gene02641.t1 AT4G36710 23.864 352 244 9 222 565 150 485 3.36e-16 82.0

Find 115 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTATGAGAATAAGAAATT+TGG 0.178504 3.1:-72220633 None:intergenic
GGTACAACCATGTCATTAAT+TGG 0.248675 3.1:+72220526 None:intergenic
CCATGTAGGCTGCAATTCTT+TGG 0.255636 3.1:-72221230 None:intergenic
GAAAAGGGTGCACTTCATTT+TGG 0.257256 3.1:+72222167 MS.gene02641:CDS
CACCAAATCTGATCCGAAAT+TGG 0.280682 3.1:-72220803 None:intergenic
GGTATGAAGTCGCGGGAAAA+TGG 0.287860 3.1:+72222033 MS.gene02641:CDS
GCTACTGATGCTGCTAATAT+TGG 0.307471 3.1:-72221061 None:intergenic
TCCACGACCGTTACAAGTTT+TGG 0.308347 3.1:-72221816 None:intergenic
AAGAAATAACGAAAATTTCT+AGG 0.316566 3.1:+72221091 MS.gene02641:CDS
GTCCAATTTCGGATCAGATT+TGG 0.328151 3.1:+72220801 MS.gene02641:CDS
TCGAAGCAAGTGTTTGAATC+AGG 0.332729 3.1:-72221488 None:intergenic
TTGATCCTAACATTATTGAT+TGG 0.340862 3.1:+72220962 MS.gene02641:CDS
CTTATGAGAATAAGAAATTT+GGG 0.359390 3.1:-72220632 None:intergenic
TCAGCACCACGTTGCCTTGT+TGG 0.374411 3.1:-72220775 None:intergenic
GGTGCTGATCAGTCCAATTT+CGG 0.378562 3.1:+72220790 MS.gene02641:CDS
TCACTCACATTTGTGCTCAT+TGG 0.383131 3.1:-72222092 None:intergenic
GTACAAGATAAAGGAGGAAA+AGG 0.383813 3.1:+72222151 MS.gene02641:CDS
GCCGCTCGCCTCGCTGAATC+CGG 0.387906 3.1:+72221264 MS.gene02641:CDS
ATTGAGGAAGAAGAGGAATA+TGG 0.391911 3.1:+72220919 MS.gene02641:CDS
TGATCCTAACATTATTGATT+GGG 0.392056 3.1:+72220963 MS.gene02641:CDS
CGACGAATTGTCTCAATTCA+AGG 0.402603 3.1:+72221198 MS.gene02641:CDS
GGCAAGGGATATTGTTAATA+CGG 0.403210 3.1:+72221980 MS.gene02641:CDS
CATTCATAAAGAAGTTGCTT+GGG 0.414263 3.1:-72221117 None:intergenic
AACAGCAGCATATTCTGAAT+TGG 0.421315 3.1:-72220997 None:intergenic
CTGTGATAGGTACAAGATAA+AGG 0.425096 3.1:+72222142 MS.gene02641:CDS
GGGTTAACATCGATTTCCTT+TGG 0.428305 3.1:-72220598 None:intergenic
GTGAATTCAATTGAAGTTGA+TGG 0.432801 3.1:+72220841 MS.gene02641:CDS
TCAGCCCAATCAATAATGTT+AGG 0.434921 3.1:-72220967 None:intergenic
ACCGTTGAAACGCTCTCGTC+AGG 0.440496 3.1:-72221750 None:intergenic
CCATCACATGTTAGATTGAC+CGG 0.440788 3.1:+72221522 MS.gene02641:CDS
ATGCTGCTAATATTGGAATC+TGG 0.446519 3.1:-72221054 None:intergenic
CATGTAGGCTGCAATTCTTT+GGG 0.448246 3.1:-72221229 None:intergenic
ATGCAAGGAACCTCCTTCTT+CGG 0.450058 3.1:+72221311 MS.gene02641:CDS
AAGTTAAGTTGTACTGTGAT+AGG 0.450787 3.1:+72222129 MS.gene02641:CDS
CCAAAGAATTGCAGCCTACA+TGG 0.451697 3.1:+72221230 MS.gene02641:CDS
GAGATCAAAACTAGAAGCAT+TGG 0.459866 3.1:+72220870 MS.gene02641:CDS
TCTGGAGACGAATACTCCTT+TGG 0.473491 3.1:-72221036 None:intergenic
TTCGGATCAGATTTGGTGAT+GGG 0.474390 3.1:+72220808 MS.gene02641:CDS
TGATTCAAACACTTGCTTCG+AGG 0.476019 3.1:+72221490 MS.gene02641:CDS
TACTCTTCCTAGAGAGAGCC+AGG 0.477900 3.1:+72221929 MS.gene02641:CDS
AGCATATTCTGAATTGGATC+AGG 0.480387 3.1:-72220991 None:intergenic
CGTCAGGCATGTGATGAAGC+TGG 0.484030 3.1:-72221734 None:intergenic
TTGCATGTGAAGGTGAAGAT+AGG 0.487421 3.1:+72222003 MS.gene02641:CDS
TCTCAACATTCATCCTATCC+TGG 0.491515 3.1:-72221947 None:intergenic
GTTGGAGGACTAAAGATCAT+AGG 0.492091 3.1:+72221576 MS.gene02641:CDS
AGCGATGCAGATACTGTTCG+AGG 0.503528 3.1:+72221344 MS.gene02641:CDS
ACGAGACCAACTTCTTCGCT+TGG 0.503960 3.1:+72221779 MS.gene02641:CDS
ACATTCATAAAGAAGTTGCT+TGG 0.509000 3.1:-72221118 None:intergenic
ATCCTATCCTGGCTCTCTCT+AGG 0.512100 3.1:-72221936 None:intergenic
TCTTTACCAAGCGAAGAAGT+TGG 0.520115 3.1:-72221785 None:intergenic
TCGCGGGAAAATGGAGAGCT+AGG 0.521293 3.1:+72222042 MS.gene02641:CDS
GCTGCTAGACGGTCCGAAGA+AGG 0.532544 3.1:-72221324 None:intergenic
TCTGTGCAGCGATCCGTTGG+AGG 0.533052 3.1:+72221561 MS.gene02641:CDS
CGAAGCAAGTGTTTGAATCA+GGG 0.537450 3.1:-72221487 None:intergenic
GTAGAGATGTCAGAACTAGA+TGG 0.537559 3.1:-72220742 None:intergenic
GAGTCTGTGCAGCGATCCGT+TGG 0.538684 3.1:+72221558 MS.gene02641:CDS
TCCAAAACTTGTAACGGTCG+TGG 0.541218 3.1:+72221815 MS.gene02641:CDS
TTATAGAGCTTTGAAATGCA+AGG 0.546542 3.1:+72221296 MS.gene02641:CDS
TACAAGATAAAGGAGGAAAA+GGG 0.547340 3.1:+72222152 MS.gene02641:CDS
AGAGCTAGGATGACAATGGC+TGG 0.548519 3.1:+72222056 MS.gene02641:CDS
TTTGAATCCAAAACTTGTAA+CGG 0.552516 3.1:+72221809 MS.gene02641:CDS
ACTTGATTGTTGCTTCAGCA+TGG 0.554425 3.1:+72222204 MS.gene02641:CDS
TAGTGACATTGAGGAAGAAG+AGG 0.556415 3.1:+72220912 MS.gene02641:CDS
ACGGATCGCTGCACAGACTC+AGG 0.557758 3.1:-72221555 None:intergenic
GAACAACTAGCAGAAACACT+TGG 0.558635 3.1:+72221609 MS.gene02641:CDS
ACTTGATGATAGTGACATTG+AGG 0.558721 3.1:+72220903 MS.gene02641:CDS
CCTTTGGTGAGTCATGATCA+TGG 0.558992 3.1:-72221020 None:intergenic
TTTCGGATCAGATTTGGTGA+TGG 0.559466 3.1:+72220807 MS.gene02641:CDS
ATATGGTAATTCTCAAAGCA+TGG 0.562265 3.1:+72220936 MS.gene02641:CDS
CCGGTCAATCTAACATGTGA+TGG 0.566500 3.1:-72221522 None:intergenic
CATCACATGTTAGATTGACC+GGG 0.567991 3.1:+72221523 MS.gene02641:CDS
CTCGTAACTCTCGCTCGAGT+AGG 0.572630 3.1:-72220680 None:intergenic
TTGAGAGGCAATGCTTGGCA+AGG 0.577561 3.1:+72221964 MS.gene02641:CDS
AATTGCAGCCTACATGGTGG+AGG 0.577669 3.1:+72221236 MS.gene02641:CDS
TAGGCTGCAATTCTTTGGGA+AGG 0.583102 3.1:-72221225 None:intergenic
CAAAACTAGAAGCATTGGAG+AGG 0.583413 3.1:+72220875 MS.gene02641:CDS
GAATGTTGAGAGGCAATGCT+TGG 0.583862 3.1:+72221959 MS.gene02641:CDS
CTACAAGTTTCATGCCAACA+AGG 0.584293 3.1:+72220761 MS.gene02641:CDS
TGTGCTACTGCAATTTCAGA+AGG 0.584377 3.1:+72221138 MS.gene02641:CDS
GCCTGACGAGAGCGTTTCAA+CGG 0.590858 3.1:+72221749 MS.gene02641:CDS
TCCGGATTCAGCGAGGCGAG+CGG 0.592435 3.1:-72221265 None:intergenic
CACTAAGCTCAAATCGCCAA+AGG 0.608012 3.1:+72220582 MS.gene02641:CDS
TATGATCTTTAGTCCTCCAA+CGG 0.609253 3.1:-72221574 None:intergenic
GTATCTGCATCGCTGCTAGA+CGG 0.609923 3.1:-72221335 None:intergenic
ATTCATAAAGAAGTTGCTTG+GGG 0.610084 3.1:-72221116 None:intergenic
GTTAATACGGTTGCATGTGA+AGG 0.612068 3.1:+72221993 MS.gene02641:CDS
ATAGAACGGTATGAAGTCGC+GGG 0.612894 3.1:+72222026 MS.gene02641:CDS
AGACTCAGGATCATCAATCC+CGG 0.614792 3.1:-72221541 None:intergenic
TAACTATGCAATAACAGAAG+CGG 0.616342 3.1:+72221401 MS.gene02641:CDS
GATAGAACGGTATGAAGTCG+CGG 0.616637 3.1:+72222025 MS.gene02641:CDS
TCGTCGTGGAGAAGCTCTTG+TGG 0.619304 3.1:+72221698 MS.gene02641:CDS
AAGGTGAAGATAGGATAGAA+CGG 0.625100 3.1:+72222012 MS.gene02641:CDS
TTCCTAGAGAGAGCCAGGAT+AGG 0.625909 3.1:+72221934 MS.gene02641:CDS
TGAGAGGCAATGCTTGGCAA+GGG 0.635494 3.1:+72221965 MS.gene02641:CDS
TCGATGCTTGATTGTCGTCG+TGG 0.642078 3.1:+72221684 MS.gene02641:CDS
AAAACTAGAAGCATTGGAGA+GGG 0.649054 3.1:+72220876 MS.gene02641:CDS
AGAAGATCCAATTAATGACA+TGG 0.650375 3.1:-72220533 None:intergenic
ATTGCAGCCTACATGGTGGA+GGG 0.650477 3.1:+72221237 MS.gene02641:CDS
GGCGAGTCCCTCCACCATGT+AGG 0.652264 3.1:-72221244 None:intergenic
GCTAGACGGTCCGAAGAAGG+AGG 0.655079 3.1:-72221321 None:intergenic
TTCATAAAGAAGTTGCTTGG+GGG 0.656482 3.1:-72221115 None:intergenic
CCATGATCATGACTCACCAA+AGG 0.657257 3.1:+72221020 MS.gene02641:CDS
ATGAGAATAAGAAATTTGGG+AGG 0.658872 3.1:-72220629 None:intergenic
AGGATAGGATGAATGTTGAG+AGG 0.660140 3.1:+72221949 MS.gene02641:CDS
AATTTCAGAAGGAAATGAAG+AGG 0.662867 3.1:+72221149 MS.gene02641:CDS
ATGGAGAGCTAGGATGACAA+TGG 0.667953 3.1:+72222052 MS.gene02641:CDS
CTTGAATTGAGACAATTCGT+CGG 0.672967 3.1:-72221197 None:intergenic
AATTTCGAGCGGTCTCATCG+AGG 0.679065 3.1:+72221643 MS.gene02641:CDS
TTTGTCATTTGAATTTCGAG+CGG 0.680630 3.1:+72221632 MS.gene02641:CDS
AAGAATTGCAGCCTACATGG+TGG 0.682524 3.1:+72221233 MS.gene02641:CDS
TGATAGGTACAAGATAAAGG+AGG 0.692576 3.1:+72222145 MS.gene02641:CDS
ACGACAATCAAGCATCGATG+AGG 0.692866 3.1:-72221679 None:intergenic
ATAAACCCGAATTTAAAACA+CGG 0.716869 3.1:-72221372 None:intergenic
TTCATGCCAACAAGGCAACG+TGG 0.722675 3.1:+72220769 MS.gene02641:CDS
TGATTGTTGCTTCAGCATGG+AGG 0.748962 3.1:+72222207 MS.gene02641:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAATAACGAAAATTTCT+AGG + chr3.1:72221091-72221110 MS.gene02641:CDS 20.0%
!! CTTATGAGAATAAGAAATTT+GGG - chr3.1:72220635-72220654 None:intergenic 20.0%
!!! AGAAATTTTCGTTATTTCTT+TGG - chr3.1:72221091-72221110 None:intergenic 20.0%
!!! ATAGATTTTGATATCAATCA+AGG + chr3.1:72221450-72221469 MS.gene02641:CDS 20.0%
! GCTTATGAGAATAAGAAATT+TGG - chr3.1:72220636-72220655 None:intergenic 25.0%
! TTGATCCTAACATTATTGAT+TGG + chr3.1:72220962-72220981 MS.gene02641:CDS 25.0%
! TTTGAATCCAAAACTTGTAA+CGG + chr3.1:72221809-72221828 MS.gene02641:CDS 25.0%
!! ATAAACCCGAATTTAAAACA+CGG - chr3.1:72221375-72221394 None:intergenic 25.0%
!! TGATCCTAACATTATTGATT+GGG + chr3.1:72220963-72220982 MS.gene02641:CDS 25.0%
!!! AATTTTCGTTATTTCTTTGG+TGG - chr3.1:72221088-72221107 None:intergenic 25.0%
!!! AGCTCTATAAATACTTTTTC+CGG - chr3.1:72221286-72221305 None:intergenic 25.0%
!!! AGTACAACTTAACTTGTTTT+CGG - chr3.1:72222124-72222143 None:intergenic 25.0%
AAATTTGGGAGGAAAAATCA+GGG - chr3.1:72220621-72220640 None:intergenic 30.0%
AATTTCAGAAGGAAATGAAG+AGG + chr3.1:72221149-72221168 MS.gene02641:CDS 30.0%
ACATTCATAAAGAAGTTGCT+TGG - chr3.1:72221121-72221140 None:intergenic 30.0%
ATATGGTAATTCTCAAAGCA+TGG + chr3.1:72220936-72220955 MS.gene02641:CDS 30.0%
ATGAGAATAAGAAATTTGGG+AGG - chr3.1:72220632-72220651 None:intergenic 30.0%
ATTCATAAAGAAGTTGCTTG+GGG - chr3.1:72221119-72221138 None:intergenic 30.0%
CATTCATAAAGAAGTTGCTT+GGG - chr3.1:72221120-72221139 None:intergenic 30.0%
GTGAATTCAATTGAAGTTGA+TGG + chr3.1:72220841-72220860 MS.gene02641:CDS 30.0%
TAACTATGCAATAACAGAAG+CGG + chr3.1:72221401-72221420 MS.gene02641:CDS 30.0%
TACAAGATAAAGGAGGAAAA+GGG + chr3.1:72222152-72222171 MS.gene02641:CDS 30.0%
TCAACAAATCTCTGCAAAAA+CGG - chr3.1:72221867-72221886 None:intergenic 30.0%
! AAGTTAAGTTGTACTGTGAT+AGG + chr3.1:72222129-72222148 MS.gene02641:CDS 30.0%
! TTTGTCATTTGAATTTCGAG+CGG + chr3.1:72221632-72221651 MS.gene02641:CDS 30.0%
!! GTTTGTCCGTGTTTTAAATT+CGG + chr3.1:72221366-72221385 MS.gene02641:CDS 30.0%
!! TTATAGAGCTTTGAAATGCA+AGG + chr3.1:72221296-72221315 MS.gene02641:CDS 30.0%
!! TTTGTCCGTGTTTTAAATTC+GGG + chr3.1:72221367-72221386 MS.gene02641:CDS 30.0%
AACAAATCTCTGCAAAAACG+GGG - chr3.1:72221865-72221884 None:intergenic 35.0%
AACAGCAGCATATTCTGAAT+TGG - chr3.1:72221000-72221019 None:intergenic 35.0%
AAGGTGAAGATAGGATAGAA+CGG + chr3.1:72222012-72222031 MS.gene02641:CDS 35.0%
AGCATATTCTGAATTGGATC+AGG - chr3.1:72220994-72221013 None:intergenic 35.0%
ATGCTGCTAATATTGGAATC+TGG - chr3.1:72221057-72221076 None:intergenic 35.0%
ATTGAGGAAGAAGAGGAATA+TGG + chr3.1:72220919-72220938 MS.gene02641:CDS 35.0%
CAACAAATCTCTGCAAAAAC+GGG - chr3.1:72221866-72221885 None:intergenic 35.0%
CTGTGATAGGTACAAGATAA+AGG + chr3.1:72222142-72222161 MS.gene02641:CDS 35.0%
CTTGAATTGAGACAATTCGT+CGG - chr3.1:72221200-72221219 None:intergenic 35.0%
GAAATTTGGGAGGAAAAATC+AGG - chr3.1:72220622-72220641 None:intergenic 35.0%
GGCAAGGGATATTGTTAATA+CGG + chr3.1:72221980-72221999 MS.gene02641:CDS 35.0%
GTACAAGATAAAGGAGGAAA+AGG + chr3.1:72222151-72222170 MS.gene02641:CDS 35.0%
TATGATCTTTAGTCCTCCAA+CGG - chr3.1:72221577-72221596 None:intergenic 35.0%
TCAGCCCAATCAATAATGTT+AGG - chr3.1:72220970-72220989 None:intergenic 35.0%
TGATAGGTACAAGATAAAGG+AGG + chr3.1:72222145-72222164 MS.gene02641:CDS 35.0%
TTCATAAAGAAGTTGCTTGG+GGG - chr3.1:72221118-72221137 None:intergenic 35.0%
! ACTTGATGATAGTGACATTG+AGG + chr3.1:72220903-72220922 MS.gene02641:CDS 35.0%
! AGCTTAGTGCTTTTATGTGA+TGG - chr3.1:72220571-72220590 None:intergenic 35.0%
! GAGATCAAAACTAGAAGCAT+TGG + chr3.1:72220870-72220889 MS.gene02641:CDS 35.0%
! TTAGTGCTTTTATGTGATGG+TGG - chr3.1:72220568-72220587 None:intergenic 35.0%
!! AAAACTAGAAGCATTGGAGA+GGG + chr3.1:72220876-72220895 MS.gene02641:CDS 35.0%
AGGATAGGATGAATGTTGAG+AGG + chr3.1:72221949-72221968 MS.gene02641:CDS 40.0%
CACCAAATCTGATCCGAAAT+TGG - chr3.1:72220806-72220825 None:intergenic 40.0%
CATCACATGTTAGATTGACC+GGG + chr3.1:72221523-72221542 MS.gene02641:CDS 40.0%
CATGTAGGCTGCAATTCTTT+GGG - chr3.1:72221232-72221251 None:intergenic 40.0%
CCATCACATGTTAGATTGAC+CGG + chr3.1:72221522-72221541 MS.gene02641:CDS 40.0%
CGAAGCAAGTGTTTGAATCA+GGG - chr3.1:72221490-72221509 None:intergenic 40.0%
CGACGAATTGTCTCAATTCA+AGG + chr3.1:72221198-72221217 MS.gene02641:CDS 40.0%
CTACAAGTTTCATGCCAACA+AGG + chr3.1:72220761-72220780 MS.gene02641:CDS 40.0%
GAACAACTAGCAGAAACACT+TGG + chr3.1:72221609-72221628 MS.gene02641:CDS 40.0%
GCTACTGATGCTGCTAATAT+TGG - chr3.1:72221064-72221083 None:intergenic 40.0%
GTAGAGATGTCAGAACTAGA+TGG - chr3.1:72220745-72220764 None:intergenic 40.0%
GTCCAATTTCGGATCAGATT+TGG + chr3.1:72220801-72220820 MS.gene02641:CDS 40.0%
GTTAATACGGTTGCATGTGA+AGG + chr3.1:72221993-72222012 MS.gene02641:CDS 40.0%
GTTGGAGGACTAAAGATCAT+AGG + chr3.1:72221576-72221595 MS.gene02641:CDS 40.0%
TAGTGACATTGAGGAAGAAG+AGG + chr3.1:72220912-72220931 MS.gene02641:CDS 40.0%
TCACTCACATTTGTGCTCAT+TGG - chr3.1:72222095-72222114 None:intergenic 40.0%
TCGAAGCAAGTGTTTGAATC+AGG - chr3.1:72221491-72221510 None:intergenic 40.0%
TCTCAACATTCATCCTATCC+TGG - chr3.1:72221950-72221969 None:intergenic 40.0%
TCTTTACCAAGCGAAGAAGT+TGG - chr3.1:72221788-72221807 None:intergenic 40.0%
TGATTCAAACACTTGCTTCG+AGG + chr3.1:72221490-72221509 MS.gene02641:CDS 40.0%
TTGCATGTGAAGGTGAAGAT+AGG + chr3.1:72222003-72222022 MS.gene02641:CDS 40.0%
! ACTTGATTGTTGCTTCAGCA+TGG + chr3.1:72222204-72222223 MS.gene02641:CDS 40.0%
! TGTGCTACTGCAATTTCAGA+AGG + chr3.1:72221138-72221157 MS.gene02641:CDS 40.0%
!! CAAAACTAGAAGCATTGGAG+AGG + chr3.1:72220875-72220894 MS.gene02641:CDS 40.0%
!! GAAAAGGGTGCACTTCATTT+TGG + chr3.1:72222167-72222186 MS.gene02641:CDS 40.0%
!! GGGTTAACATCGATTTCCTT+TGG - chr3.1:72220601-72220620 None:intergenic 40.0%
!! TTCGGATCAGATTTGGTGAT+GGG + chr3.1:72220808-72220827 MS.gene02641:CDS 40.0%
!! TTTCGGATCAGATTTGGTGA+TGG + chr3.1:72220807-72220826 MS.gene02641:CDS 40.0%
AAATCTCTGCAAAAACGGGG+CGG - chr3.1:72221862-72221881 None:intergenic 45.0%
AAGAATTGCAGCCTACATGG+TGG + chr3.1:72221233-72221252 MS.gene02641:CDS 45.0%
AGACTCAGGATCATCAATCC+CGG - chr3.1:72221544-72221563 None:intergenic 45.0%
ATAGAACGGTATGAAGTCGC+GGG + chr3.1:72222026-72222045 MS.gene02641:CDS 45.0%
ATGCAAGGAACCTCCTTCTT+CGG + chr3.1:72221311-72221330 MS.gene02641:CDS 45.0%
ATGGAGAGCTAGGATGACAA+TGG + chr3.1:72222052-72222071 MS.gene02641:CDS 45.0%
CACTAAGCTCAAATCGCCAA+AGG + chr3.1:72220582-72220601 MS.gene02641:CDS 45.0%
CCAAAGAATTGCAGCCTACA+TGG + chr3.1:72221230-72221249 MS.gene02641:CDS 45.0%
CCATGATCATGACTCACCAA+AGG + chr3.1:72221020-72221039 MS.gene02641:CDS 45.0%
CCATGTAGGCTGCAATTCTT+TGG - chr3.1:72221233-72221252 None:intergenic 45.0%
CCGGTCAATCTAACATGTGA+TGG - chr3.1:72221525-72221544 None:intergenic 45.0%
CCTTTGGTGAGTCATGATCA+TGG - chr3.1:72221023-72221042 None:intergenic 45.0%
GAATGTTGAGAGGCAATGCT+TGG + chr3.1:72221959-72221978 MS.gene02641:CDS 45.0%
GATAGAACGGTATGAAGTCG+CGG + chr3.1:72222025-72222044 MS.gene02641:CDS 45.0%
TCCAAAACTTGTAACGGTCG+TGG + chr3.1:72221815-72221834 MS.gene02641:CDS 45.0%
TCTGGAGACGAATACTCCTT+TGG - chr3.1:72221039-72221058 None:intergenic 45.0%
! TAGGCTGCAATTCTTTGGGA+AGG - chr3.1:72221228-72221247 None:intergenic 45.0%
! TCCACGACCGTTACAAGTTT+TGG - chr3.1:72221819-72221838 None:intergenic 45.0%
! TGATTGTTGCTTCAGCATGG+AGG + chr3.1:72222207-72222226 MS.gene02641:CDS 45.0%
!! ACGACAATCAAGCATCGATG+AGG - chr3.1:72221682-72221701 None:intergenic 45.0%
!! AGGGTGCACTTCATTTTGGT+TGG + chr3.1:72222171-72222190 MS.gene02641:CDS 45.0%
!! GGTGCTGATCAGTCCAATTT+CGG + chr3.1:72220790-72220809 MS.gene02641:CDS 45.0%
!!! GGGTGCACTTCATTTTGGTT+GGG + chr3.1:72222172-72222191 MS.gene02641:CDS 45.0%
!!! TACTTTTTCCGGATTCAGCG+AGG - chr3.1:72221275-72221294 None:intergenic 45.0%
AATTGCAGCCTACATGGTGG+AGG + chr3.1:72221236-72221255 MS.gene02641:CDS 50.0%
AATTTCGAGCGGTCTCATCG+AGG + chr3.1:72221643-72221662 MS.gene02641:CDS 50.0%
ACGAGACCAACTTCTTCGCT+TGG + chr3.1:72221779-72221798 MS.gene02641:CDS 50.0%
AGAGCTAGGATGACAATGGC+TGG + chr3.1:72222056-72222075 MS.gene02641:CDS 50.0%
AGCGATGCAGATACTGTTCG+AGG + chr3.1:72221344-72221363 MS.gene02641:CDS 50.0%
GGTATGAAGTCGCGGGAAAA+TGG + chr3.1:72222033-72222052 MS.gene02641:CDS 50.0%
GTATCTGCATCGCTGCTAGA+CGG - chr3.1:72221338-72221357 None:intergenic 50.0%
TACTCTTCCTAGAGAGAGCC+AGG + chr3.1:72221929-72221948 MS.gene02641:CDS 50.0%
TCGATGCTTGATTGTCGTCG+TGG + chr3.1:72221684-72221703 MS.gene02641:CDS 50.0%
TGAGAGGCAATGCTTGGCAA+GGG + chr3.1:72221965-72221984 MS.gene02641:CDS 50.0%
TTCATGCCAACAAGGCAACG+TGG + chr3.1:72220769-72220788 MS.gene02641:CDS 50.0%
TTCCTAGAGAGAGCCAGGAT+AGG + chr3.1:72221934-72221953 MS.gene02641:CDS 50.0%
TTGAGAGGCAATGCTTGGCA+AGG + chr3.1:72221964-72221983 MS.gene02641:CDS 50.0%
! ATCCTATCCTGGCTCTCTCT+AGG - chr3.1:72221939-72221958 None:intergenic 50.0%
! ATTGCAGCCTACATGGTGGA+GGG + chr3.1:72221237-72221256 MS.gene02641:CDS 50.0%
ACCGTTGAAACGCTCTCGTC+AGG - chr3.1:72221753-72221772 None:intergenic 55.0%
CGTCAGGCATGTGATGAAGC+TGG - chr3.1:72221737-72221756 None:intergenic 55.0%
CTCGTAACTCTCGCTCGAGT+AGG - chr3.1:72220683-72220702 None:intergenic 55.0%
CTGCAAAAACGGGGCGGTAT+TGG - chr3.1:72221856-72221875 None:intergenic 55.0%
TCAGCACCACGTTGCCTTGT+TGG - chr3.1:72220778-72220797 None:intergenic 55.0%
TCGCGGGAAAATGGAGAGCT+AGG + chr3.1:72222042-72222061 MS.gene02641:CDS 55.0%
TCGTCGTGGAGAAGCTCTTG+TGG + chr3.1:72221698-72221717 MS.gene02641:CDS 55.0%
!! GCCTGACGAGAGCGTTTCAA+CGG + chr3.1:72221749-72221768 MS.gene02641:CDS 55.0%
ACGGATCGCTGCACAGACTC+AGG - chr3.1:72221558-72221577 None:intergenic 60.0%
GAGTCTGTGCAGCGATCCGT+TGG + chr3.1:72221558-72221577 MS.gene02641:CDS 60.0%
GCTAGACGGTCCGAAGAAGG+AGG - chr3.1:72221324-72221343 None:intergenic 60.0%
GCTGCTAGACGGTCCGAAGA+AGG - chr3.1:72221327-72221346 None:intergenic 60.0%
TCTGTGCAGCGATCCGTTGG+AGG + chr3.1:72221561-72221580 MS.gene02641:CDS 60.0%
GGCGAGTCCCTCCACCATGT+AGG - chr3.1:72221247-72221266 None:intergenic 65.0%
TCCGGATTCAGCGAGGCGAG+CGG - chr3.1:72221268-72221287 None:intergenic 65.0%
GCCGCTCGCCTCGCTGAATC+CGG + chr3.1:72221264-72221283 MS.gene02641:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 72220535 72222232 72220535 ID=MS.gene02641
chr3.1 mRNA 72220535 72222232 72220535 ID=MS.gene02641.t1;Parent=MS.gene02641
chr3.1 exon 72220535 72222232 72220535 ID=MS.gene02641.t1.exon1;Parent=MS.gene02641.t1
chr3.1 CDS 72220535 72222232 72220535 ID=cds.MS.gene02641.t1;Parent=MS.gene02641.t1
Gene Sequence

>MS.gene02641

ATGTCATTAATTGGATCTTCTGAGCTAGCTCCACCATCACATAAAAGCACTAAGCTCAAATCGCCAAAGGAAATCGATGTTAACCCTGATTTTTCCTCCCAAATTTCTTATTCTCATAAGCATAGAAGCATGTATATCACAGATACCTACTCGAGCGAGAGTTACGAGAAGTACTTCAATGACTCACAGACAGAAGAACTAATAGAACCATCTAGTTCTGACATCTCTACAAGTTTCATGCCAACAAGGCAACGTGGTGCTGATCAGTCCAATTTCGGATCAGATTTGGTGATGGGAAATCGAAGCGTGAATTCAATTGAAGTTGATGGTGCAATGAGATCAAAACTAGAAGCATTGGAGAGGGCATTACTTGATGATAGTGACATTGAGGAAGAAGAGGAATATGGTAATTCTCAAAGCATGGAAATTGATCCTAACATTATTGATTGGGCTGATCCTGATCCAATTCAGAATATGCTGCTGTTCCATGATCATGACTCACCAAAGGAGTATTCGTCTCCAGATTCCAATATTAGCAGCATCAGTAGCACCACCAAAGAAATAACGAAAATTTCTAGGACCCCCAAGCAACTTCTTTATGAATGTGCTACTGCAATTTCAGAAGGAAATGAAGAGGAAGCATCGTCAATGATAAACAATCTCCGACGAATTGTCTCAATTCAAGGAGAGCCTTCCCAAAGAATTGCAGCCTACATGGTGGAGGGACTCGCCGCTCGCCTCGCTGAATCCGGAAAAAGTATTTATAGAGCTTTGAAATGCAAGGAACCTCCTTCTTCGGACCGTCTAGCAGCGATGCAGATACTGTTCGAGGTTTGTCCGTGTTTTAAATTCGGGTTTATTGCAGCTAACTATGCAATAACAGAAGCGGTTAAAGATGAAATGAAGATTCATATAATAGATTTTGATATCAATCAAGGAAGTCAGTACATAACCCTGATTCAAACACTTGCTTCGAGGTCTTGTAAGCCATCACATGTTAGATTGACCGGGATTGATGATCCTGAGTCTGTGCAGCGATCCGTTGGAGGACTAAAGATCATAGGACAAAGACTTGAACAACTAGCAGAAACACTTGGTTTGTCATTTGAATTTCGAGCGGTCTCATCGAGGACTTCAATTGTCACCTCATCGATGCTTGATTGTCGTCGTGGAGAAGCTCTTGTGGTGAACTTTGCATTCCAGCTTCATCACATGCCTGACGAGAGCGTTTCAACGGTGAACGAACGAGACCAACTTCTTCGCTTGGTAAAGAGTTTGAATCCAAAACTTGTAACGGTCGTGGAGCAAGATGTAAACACCAATACCGCCCCGTTTTTGCAGAGATTTGTTGAAGCTTACAATTACTACTCTGCTGTGTTTGAGTCACTTGATGTTACTCTTCCTAGAGAGAGCCAGGATAGGATGAATGTTGAGAGGCAATGCTTGGCAAGGGATATTGTTAATACGGTTGCATGTGAAGGTGAAGATAGGATAGAACGGTATGAAGTCGCGGGAAAATGGAGAGCTAGGATGACAATGGCTGGATTCAGTTCATCACCAATGAGCACAAATGTGAGTGATGAAATCCGAAAACAAGTTAAGTTGTACTGTGATAGGTACAAGATAAAGGAGGAAAAGGGTGCACTTCATTTTGGTTGGGAAGACAAAAACTTGATTGTTGCTTCAGCATGGAGGTGA
Protein sequence

>MS.gene02641.t1

MSLIGSSELAPPSHKSTKLKSPKEIDVNPDFSSQISYSHKHRSMYITDTYSSESYEKYFNDSQTEELIEPSSSDISTSFMPTRQRGADQSNFGSDLVMGNRSVNSIEVDGAMRSKLEALERALLDDSDIEEEEEYGNSQSMEIDPNIIDWADPDPIQNMLLFHDHDSPKEYSSPDSNISSISSTTKEITKISRTPKQLLYECATAISEGNEEEASSMINNLRRIVSIQGEPSQRIAAYMVEGLAARLAESGKSIYRALKCKEPPSSDRLAAMQILFEVCPCFKFGFIAANYAITEAVKDEMKIHIIDFDINQGSQYITLIQTLASRSCKPSHVRLTGIDDPESVQRSVGGLKIIGQRLEQLAETLGLSFEFRAVSSRTSIVTSSMLDCRRGEALVVNFAFQLHHMPDESVSTVNERDQLLRLVKSLNPKLVTVVEQDVNTNTAPFLQRFVEAYNYYSAVFESLDVTLPRESQDRMNVERQCLARDIVNTVACEGEDRIERYEVAGKWRARMTMAGFSSSPMSTNVSDEIRKQVKLYCDRYKIKEEKGALHFGWEDKNLIVASAWR