AlfalfaGEDB | MS.gene026444

M. sativa cultivar XinJiangDaYe / MS.gene026444

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00249.34

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene026444.t1	KEH40705.1	97.8	135	3	0	1	135	1	135	2.00E-54	221.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene026444.t1	Q9FNN6	67.2	137	40	3	1	137	1	132	1.4e-29	130.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene026444.t1	A0A072VRE4	97.8	135	3	0	1	135	1	135	1.4e-54	221.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene026444.t1	TF	MYB-related

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene026444.t1	MTR_1g033600	97.778	135	3	0	1	135	1	135	1.57e-96	274
MS.gene026444.t1	MTR_8g063600	58.519	135	41	2	3	137	1	120	4.27e-45	149
MS.gene026444.t1	MTR_8g063870	50.370	135	38	4	3	137	1	106	7.58e-31	112
MS.gene026444.t1	MTR_4g111975	46.575	146	57	6	12	137	8	152	6.14e-29	107
MS.gene026444.t1	MTR_3g462790	38.060	134	76	2	4	137	35	161	1.40e-28	107
MS.gene026444.t1	MTR_7g067080	41.007	139	60	3	7	137	30	154	4.58e-27	102
MS.gene026444.t1	MTR_5g081860	44.531	128	58	5	10	137	25	139	1.46e-25	98.2
MS.gene026444.t1	MTR_1g022290	51.807	83	40	0	6	88	1	83	2.13e-25	92.8
MS.gene026444.t1	MTR_1g111830	43.939	132	55	3	10	137	22	138	4.18e-25	96.3
MS.gene026444.t1	MTR_5g069710	38.060	134	65	3	9	137	26	146	1.46e-23	93.6
MS.gene026444.t1	MTR_3g111880	55.072	69	31	0	9	77	4	72	2.60e-21	82.4
MS.gene026444.t1	MTR_3g116720	50.000	68	34	0	10	77	14	81	3.73e-20	79.3
MS.gene026444.t1	MTR_5g020170	48.684	76	39	0	7	82	14	89	6.57e-20	79.0
MS.gene026444.t1	MTR_7g089210	56.250	64	28	0	15	78	15	78	2.00e-19	77.4
MS.gene026444.t1	MTR_6g004250	50.685	73	36	0	3	75	1	73	3.52e-19	76.6
MS.gene026444.t1	MTR_3g111920	41.791	67	39	0	12	78	3	69	8.60e-17	70.9
MS.gene026444.t1	MTR_8g077360	39.726	73	43	1	9	81	2	73	5.33e-16	68.2
MS.gene026444.t1	MTR_8g077380	40.000	70	41	1	9	78	2	70	1.48e-15	67.0
MS.gene026444.t1	MTR_8g077390	40.000	70	41	1	9	78	2	70	1.48e-15	67.0
MS.gene026444.t1	MTR_3g013440	45.205	73	39	1	3	74	1	73	2.09e-15	67.4
MS.gene026444.t1	MTR_8g077420	42.857	63	35	1	10	72	3	64	1.40e-13	62.0
MS.gene026444.t1	MTR_5g020540	46.032	63	33	1	10	72	2	63	3.45e-13	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene026444.t1	AT5G08520	62.044	137	47	5	1	137	1	132	1.84e-49	160
MS.gene026444.t1	AT2G38090	39.098	133	78	2	7	137	24	155	3.00e-26	100
MS.gene026444.t1	AT5G05790	43.077	130	61	4	9	137	28	145	7.46e-26	99.0
MS.gene026444.t1	AT5G05790	43.077	130	61	4	9	137	28	145	7.46e-26	99.0
MS.gene026444.t1	AT3G11280	40.945	127	56	3	12	137	33	141	5.79e-25	96.3
MS.gene026444.t1	AT3G11280	40.945	127	56	3	12	137	33	141	5.79e-25	96.3
MS.gene026444.t1	AT5G58900	40.769	130	69	4	10	137	32	155	7.50e-25	96.7
MS.gene026444.t1	AT5G23650	41.732	127	71	2	5	131	6	129	4.39e-24	95.5
MS.gene026444.t1	AT4G39250	51.948	77	37	0	12	88	14	90	4.58e-23	87.4
MS.gene026444.t1	AT1G49010	39.310	145	69	4	10	137	6	148	6.18e-23	92.0
MS.gene026444.t1	AT1G75250	50.000	74	37	0	10	83	10	83	8.00e-22	84.0
MS.gene026444.t1	AT1G75250	50.000	74	37	0	10	83	10	83	1.61e-21	84.0
MS.gene026444.t1	AT2G21650	51.429	70	34	0	12	81	14	83	7.03e-19	76.6
MS.gene026444.t1	AT1G19510	45.946	74	40	0	15	88	15	88	1.00e-18	76.3
MS.gene026444.t1	AT5G01200	35.000	140	72	5	10	137	28	160	2.74e-18	79.0
MS.gene026444.t1	AT2G18328	50.794	63	31	0	16	78	15	77	3.03e-17	71.6
MS.gene026444.t1	AT5G04760	34.109	129	67	4	9	137	3	113	3.83e-16	72.4
MS.gene026444.t1	AT3G10580	30.597	134	67	3	12	143	8	117	7.24e-11	58.5
MS.gene026444.t1	AT3G10580	30.597	134	67	3	12	143	8	117	8.12e-11	58.5

Find 43 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCAACAAGACTATGACTA+TGG	0.261298	1.1:+18543018	None:intergenic
AGTAATCAAAATTGGCATAA+AGG	0.323067	1.1:-18543438	None:intergenic
AAGTTAAAAGTAATCAAAAT+TGG	0.330013	1.1:-18543446	None:intergenic
CGAGTCTAACCATGGAACTA+AGG	0.427854	1.1:+18543339	MS.gene026444:CDS
ATGCTAGCGATGAAGGAGTT+GGG	0.443412	1.1:+18543281	MS.gene026444:CDS
TAAGCAGCATTATGAGCTCT+TGG	0.453346	1.1:+18543180	MS.gene026444:CDS
ACAATAGCTCTGAGTGGAGC+TGG	0.456097	1.1:+18543050	MS.gene026444:CDS
AAGATTGCGGCCGATGTACC+AGG	0.464527	1.1:+18543139	MS.gene026444:CDS
ATTTCTTCCATAGTCTTCCC+TGG	0.467613	1.1:-18543157	None:intergenic
ACAAGTCATGCTAGCGATGA+AGG	0.470619	1.1:+18543274	MS.gene026444:CDS
CGATGTACCAGGGAAGACTA+TGG	0.470839	1.1:+18543150	MS.gene026444:CDS
GTACACAACCAGCTTCAATC+TGG	0.495059	1.1:-18543216	None:intergenic
CTGAGGATGATTCGGATCGA+TGG	0.497129	1.1:+18543113	MS.gene026444:CDS
ATCTTGAGCCCTTAGTTCCA+TGG	0.501596	1.1:-18543348	None:intergenic
GACTATGACTATGGATGAAG+TGG	0.502959	1.1:+18543027	None:intergenic
CATAGTCTTCCCTGGTACAT+CGG	0.502968	1.1:-18543149	None:intergenic
GACGTATCCTGAGGATGATT+CGG	0.503412	1.1:+18543105	MS.gene026444:CDS
CATGCTAGCGATGAAGGAGT+TGG	0.506146	1.1:+18543280	MS.gene026444:CDS
AACGACGAAAGGGTATTGCT+TGG	0.523028	1.1:+18543380	MS.gene026444:CDS
GTCGTATAATTCTTCTTCAG+AGG	0.524347	1.1:+18543246	MS.gene026444:CDS
TAAGGCATTTGAAAATGCCT+TGG	0.529052	1.1:+18543081	MS.gene026444:CDS
TCGTATAATTCTTCTTCAGA+GGG	0.532506	1.1:+18543247	MS.gene026444:CDS
TGAGGATGATTCGGATCGAT+GGG	0.549268	1.1:+18543114	MS.gene026444:CDS
CATCGATCCGAATCATCCTC+AGG	0.553968	1.1:-18543112	None:intergenic
TGCTAGCGATGAAGGAGTTG+GGG	0.556566	1.1:+18543282	MS.gene026444:CDS
GATATTAGCCAGATTGAAGC+TGG	0.559375	1.1:+18543208	MS.gene026444:CDS
GGGAAGAAGGGTGGTCACAA+TGG	0.559678	1.1:+18543304	MS.gene026444:CDS
ATCTGATCAAGAACGACGAA+AGG	0.561791	1.1:+18543369	MS.gene026444:CDS
CAATAGCTCTGAGTGGAGCT+GGG	0.561880	1.1:+18543051	MS.gene026444:CDS
TCTGATCAAGAACGACGAAA+GGG	0.570329	1.1:+18543370	MS.gene026444:CDS
GTGGAGCTGGGAGCAAGATA+AGG	0.570448	1.1:+18543063	MS.gene026444:CDS
GAGTCTAACCATGGAACTAA+GGG	0.584526	1.1:+18543340	MS.gene026444:CDS
GAAGAATTATACGACGGTAG+AGG	0.596110	1.1:-18543238	None:intergenic
AAAGGGTATTGCTTGGACTG+AGG	0.609474	1.1:+18543387	MS.gene026444:CDS
GGATCGATGGGAGAAGATTG+CGG	0.637780	1.1:+18543126	MS.gene026444:CDS
TGCCTTGGCGACGTATCCTG+AGG	0.642436	1.1:+18543096	MS.gene026444:CDS
GCTAGCGATGAAGGAGTTGG+GGG	0.644660	1.1:+18543283	MS.gene026444:CDS
ATCCTCAGGATACGTCGCCA+AGG	0.650046	1.1:-18543098	None:intergenic
AGATTGCGGCCGATGTACCA+GGG	0.672674	1.1:+18543140	MS.gene026444:CDS
AAGTGGACAATAGCTCTGAG+TGG	0.698553	1.1:+18543044	MS.gene026444:CDS
CTTGGACTGAGGAAGAACAC+AGG	0.712971	1.1:+18543398	MS.gene026444:CDS
CTAGCGATGAAGGAGTTGGG+GGG	0.726058	1.1:+18543284	MS.gene026444:CDS
TCTGAAGAAGAATTATACGA+CGG	0.742949	1.1:-18543244	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGTAATCAAAATTGGCATAA+AGG	-	chr1.1:18543441-18543460	None:intergenic	25.0%
	TCGTATAATTCTTCTTCAGA+GGG	+	chr1.1:18543247-18543266	MS.gene026444:CDS	30.0%
	TCTGAAGAAGAATTATACGA+CGG	-	chr1.1:18543247-18543266	None:intergenic	30.0%
	GTCGTATAATTCTTCTTCAG+AGG	+	chr1.1:18543246-18543265	MS.gene026444:CDS	35.0%
!	TAAGGCATTTGAAAATGCCT+TGG	+	chr1.1:18543081-18543100	MS.gene026444:CDS	35.0%
	AAAAACGACGAGTCTAACCA+TGG	+	chr1.1:18543331-18543350	MS.gene026444:CDS	40.0%
	ATCTGATCAAGAACGACGAA+AGG	+	chr1.1:18543369-18543388	MS.gene026444:CDS	40.0%
	ATTTCTTCCATAGTCTTCCC+TGG	-	chr1.1:18543160-18543179	None:intergenic	40.0%
	GAAGAATTATACGACGGTAG+AGG	-	chr1.1:18543241-18543260	None:intergenic	40.0%
	GAGTCTAACCATGGAACTAA+GGG	+	chr1.1:18543340-18543359	MS.gene026444:CDS	40.0%
	GATATTAGCCAGATTGAAGC+TGG	+	chr1.1:18543208-18543227	MS.gene026444:CDS	40.0%
	TAAGCAGCATTATGAGCTCT+TGG	+	chr1.1:18543180-18543199	MS.gene026444:CDS	40.0%
	TCTGATCAAGAACGACGAAA+GGG	+	chr1.1:18543370-18543389	MS.gene026444:CDS	40.0%
	ACAAGTCATGCTAGCGATGA+AGG	+	chr1.1:18543274-18543293	MS.gene026444:CDS	45.0%
	ATCTTGAGCCCTTAGTTCCA+TGG	-	chr1.1:18543351-18543370	None:intergenic	45.0%
	ATGCTAGCGATGAAGGAGTT+GGG	+	chr1.1:18543281-18543300	MS.gene026444:CDS	45.0%
	CATAGTCTTCCCTGGTACAT+CGG	-	chr1.1:18543152-18543171	None:intergenic	45.0%
	CGAGTCTAACCATGGAACTA+AGG	+	chr1.1:18543339-18543358	MS.gene026444:CDS	45.0%
	GACGTATCCTGAGGATGATT+CGG	+	chr1.1:18543105-18543124	MS.gene026444:CDS	45.0%
	GTACACAACCAGCTTCAATC+TGG	-	chr1.1:18543219-18543238	None:intergenic	45.0%
!	TGAGGATGATTCGGATCGAT+GGG	+	chr1.1:18543114-18543133	MS.gene026444:CDS	45.0%
!!	AAAGGGTATTGCTTGGACTG+AGG	+	chr1.1:18543387-18543406	MS.gene026444:CDS	45.0%
!!	AACGACGAAAGGGTATTGCT+TGG	+	chr1.1:18543380-18543399	MS.gene026444:CDS	45.0%
!!	AAGTGGACAATAGCTCTGAG+TGG	+	chr1.1:18543044-18543063	MS.gene026444:CDS	45.0%
!!!	GGTTAGACTCGTCGTTTTTG+TGG	-	chr1.1:18543330-18543349	None:intergenic	45.0%
	CATCGATCCGAATCATCCTC+AGG	-	chr1.1:18543115-18543134	None:intergenic	50.0%
	CATGCTAGCGATGAAGGAGT+TGG	+	chr1.1:18543280-18543299	MS.gene026444:CDS	50.0%
	CGATGTACCAGGGAAGACTA+TGG	+	chr1.1:18543150-18543169	MS.gene026444:CDS	50.0%
	CTTGGACTGAGGAAGAACAC+AGG	+	chr1.1:18543398-18543417	MS.gene026444:CDS	50.0%
	GGATCGATGGGAGAAGATTG+CGG	+	chr1.1:18543126-18543145	MS.gene026444:CDS	50.0%
	TGCTAGCGATGAAGGAGTTG+GGG	+	chr1.1:18543282-18543301	MS.gene026444:CDS	50.0%
!	CTGAGGATGATTCGGATCGA+TGG	+	chr1.1:18543113-18543132	MS.gene026444:CDS	50.0%
!!	ACAATAGCTCTGAGTGGAGC+TGG	+	chr1.1:18543050-18543069	MS.gene026444:CDS	50.0%
!!	CAATAGCTCTGAGTGGAGCT+GGG	+	chr1.1:18543051-18543070	MS.gene026444:CDS	50.0%
	AAGATTGCGGCCGATGTACC+AGG	+	chr1.1:18543139-18543158	MS.gene026444:CDS	55.0%
	AGATTGCGGCCGATGTACCA+GGG	+	chr1.1:18543140-18543159	MS.gene026444:CDS	55.0%
	ATCCTCAGGATACGTCGCCA+AGG	-	chr1.1:18543101-18543120	None:intergenic	55.0%
	CTAGCGATGAAGGAGTTGGG+GGG	+	chr1.1:18543284-18543303	MS.gene026444:CDS	55.0%
	GAAGGAGTTGGGGGGAAGAA+GGG	+	chr1.1:18543292-18543311	MS.gene026444:CDS	55.0%
	GCTAGCGATGAAGGAGTTGG+GGG	+	chr1.1:18543283-18543302	MS.gene026444:CDS	55.0%
	GGGAAGAAGGGTGGTCACAA+TGG	+	chr1.1:18543304-18543323	MS.gene026444:CDS	55.0%
	GTGGAGCTGGGAGCAAGATA+AGG	+	chr1.1:18543063-18543082	MS.gene026444:CDS	55.0%
	TGAAGGAGTTGGGGGGAAGA+AGG	+	chr1.1:18543291-18543310	MS.gene026444:CDS	55.0%
	TGCCTTGGCGACGTATCCTG+AGG	+	chr1.1:18543096-18543115	MS.gene026444:CDS	60.0%
	GGAGTTGGGGGGAAGAAGGG+TGG	+	chr1.1:18543295-18543314	MS.gene026444:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	18543031	18543465	18543031	ID=MS.gene026444
chr1.1	mRNA	18543031	18543465	18543031	ID=MS.gene026444.t1;Parent=MS.gene026444
chr1.1	exon	18543031	18543465	18543031	ID=MS.gene026444.t1.exon1;Parent=MS.gene026444.t1
chr1.1	CDS	18543031	18543465	18543031	ID=cds.MS.gene026444.t1;Parent=MS.gene026444.t1

Gene Sequence

>MS.gene026444

ATGACTATGGATGAAGTGGACAATAGCTCTGAGTGGAGCTGGGAGCAAGATAAGGCATTTGAAAATGCCTTGGCGACGTATCCTGAGGATGATTCGGATCGATGGGAGAAGATTGCGGCCGATGTACCAGGGAAGACTATGGAAGAAATTAAGCAGCATTATGAGCTCTTGGTTGATGATATTAGCCAGATTGAAGCTGGTTGTGTACCTCTACCGTCGTATAATTCTTCTTCAGAGGGTTCAACAAGTCATGCTAGCGATGAAGGAGTTGGGGGGAAGAAGGGTGGTCACAATGGCCACAAAAACGACGAGTCTAACCATGGAACTAAGGGCTCAAGATCTGATCAAGAACGACGAAAGGGTATTGCTTGGACTGAGGAAGAACACAGGCAAGTTTATCTCTTAAACCTTTATGCCAATTTTGATTACTTTTAA

Protein sequence

>MS.gene026444.t1

MTMDEVDNSSEWSWEQDKAFENALATYPEDDSDRWEKIAADVPGKTMEEIKQHYELLVDDISQIEAGCVPLPSYNSSSEGSTSHASDEGVGGKKGGHNGHKNDESNHGTKGSRSDQERRKGIAWTEEEHRQVYLLNLYANFDYF