Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026494.t1 | KEH40658.1 | 92.3 | 52 | 2 | 1 | 1 | 52 | 60 | 109 | 1.00E-16 | 95.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026494.t1 | A0A072VGU3 | 92.3 | 52 | 2 | 1 | 1 | 52 | 60 | 109 | 7.2e-17 | 95.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene026494.t1 | TF | PLATZ |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026494.t1 | MTR_1g032880 | 92.308 | 52 | 2 | 1 | 1 | 52 | 60 | 109 | 2.57e-27 | 95.9 |
| MS.gene026494.t1 | MTR_8g105930 | 44.118 | 68 | 22 | 1 | 1 | 52 | 90 | 157 | 1.90e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 8 sgRNAs with CRISPR-Local
Find 6 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGTTACCTTGCATTTGA+TGG | 0.360512 | 1.1:-17863889 | None:intergenic |
| TGTCTTTGCTACTGATAGTC+GGG | 0.413666 | 1.1:-17863787 | None:intergenic |
| ATGTCTTTGCTACTGATAGT+CGG | 0.457579 | 1.1:-17863788 | None:intergenic |
| ACATCTCAAAAGCAAGCTGG+TGG | 0.603751 | 1.1:+17863813 | MS.gene026494:CDS |
| TCAACATCTCAAAAGCAAGC+TGG | 0.604147 | 1.1:+17863810 | MS.gene026494:CDS |
| ATTCTGTTCCATCAAATGCA+AGG | 0.622156 | 1.1:+17863881 | MS.gene026494:CDS |
| GTCTTTGCTACTGATAGTCG+GGG | 0.635231 | 1.1:-17863786 | None:intergenic |
| GGTGGAATTTGTGAAGAATG+TGG | 0.715683 | 1.1:+17863831 | MS.gene026494:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGTCTTTGCTACTGATAGT+CGG | - | chr1.1:17863791-17863810 | None:intergenic | 35.0% | |
| GGTGGAATTTGTGAAGAATG+TGG | + | chr1.1:17863831-17863850 | MS.gene026494:CDS | 40.0% | |
| TCAACATCTCAAAAGCAAGC+TGG | + | chr1.1:17863810-17863829 | MS.gene026494:CDS | 40.0% | |
| TGTCTTTGCTACTGATAGTC+GGG | - | chr1.1:17863790-17863809 | None:intergenic | 40.0% | |
| ACATCTCAAAAGCAAGCTGG+TGG | + | chr1.1:17863813-17863832 | MS.gene026494:CDS | 45.0% | |
| GTCTTTGCTACTGATAGTCG+GGG | - | chr1.1:17863789-17863808 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 17863747 | 17863902 | 17863747 | ID=MS.gene026494 |
| chr1.1 | mRNA | 17863747 | 17863902 | 17863747 | ID=MS.gene026494.t1;Parent=MS.gene026494 |
| chr1.1 | exon | 17863747 | 17863902 | 17863747 | ID=MS.gene026494.t1.exon1;Parent=MS.gene026494.t1 |
| chr1.1 | CDS | 17863747 | 17863902 | 17863747 | ID=cds.MS.gene026494.t1;Parent=MS.gene026494.t1 |
| Gene Sequence |
| Protein sequence |
