Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027005.t1 | XP_013459842.1 | 99.7 | 306 | 1 | 0 | 1 | 306 | 1 | 306 | 1.40E-166 | 595.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027005.t1 | Q6IP65 | 67.4 | 291 | 95 | 0 | 6 | 296 | 3 | 293 | 2.0e-105 | 383.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027005.t1 | A0A072UXT2 | 99.7 | 306 | 1 | 0 | 1 | 306 | 1 | 306 | 1.0e-166 | 595.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene027005.t1 | TR | Rcd1-like |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027005.t1 | MTR_3g053170 | 99.673 | 306 | 1 | 0 | 1 | 306 | 1 | 306 | 0.0 | 624 |
| MS.gene027005.t1 | MTR_8g106940 | 60.390 | 308 | 116 | 2 | 1 | 304 | 11 | 316 | 4.14e-122 | 353 |
| MS.gene027005.t1 | MTR_6g065170 | 60.927 | 302 | 112 | 2 | 1 | 298 | 11 | 310 | 2.30e-120 | 348 |
| MS.gene027005.t1 | MTR_5g093460 | 59.220 | 282 | 114 | 1 | 16 | 297 | 157 | 437 | 1.94e-110 | 327 |
| MS.gene027005.t1 | MTR_5g093460 | 65.672 | 134 | 45 | 1 | 22 | 155 | 1 | 133 | 3.79e-50 | 172 |
| MS.gene027005.t1 | MTR_3g018640 | 57.093 | 289 | 122 | 1 | 3 | 289 | 13 | 301 | 5.67e-108 | 316 |
| MS.gene027005.t1 | MTR_1g056470 | 66.814 | 226 | 75 | 0 | 20 | 245 | 28 | 253 | 7.27e-105 | 307 |
| MS.gene027005.t1 | MTR_1g043540 | 56.000 | 125 | 54 | 1 | 130 | 254 | 9 | 132 | 8.69e-37 | 128 |
| MS.gene027005.t1 | MTR_1g043500 | 49.206 | 63 | 31 | 1 | 184 | 245 | 3 | 65 | 4.01e-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027005.t1 | AT3G20800 | 86.735 | 294 | 37 | 2 | 12 | 304 | 24 | 316 | 0.0 | 520 |
| MS.gene027005.t1 | AT5G12980 | 78.694 | 291 | 56 | 2 | 17 | 306 | 26 | 311 | 3.78e-169 | 471 |
| MS.gene027005.t1 | AT2G32550 | 34.432 | 273 | 163 | 5 | 27 | 286 | 51 | 320 | 8.03e-44 | 152 |
| MS.gene027005.t1 | AT2G32550 | 35.060 | 251 | 149 | 4 | 47 | 286 | 34 | 281 | 2.01e-42 | 147 |
| MS.gene027005.t1 | AT2G32550 | 34.934 | 229 | 135 | 4 | 69 | 286 | 1 | 226 | 2.76e-37 | 132 |
Find 73 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCGCTGTTGGTCGAGTTCT+TGG | 0.255938 | 3.4:-49943889 | MS.gene027005:CDS |
| CGCTCTTCTCGAACTCTCTA+AGG | 0.260230 | 3.4:-49950278 | MS.gene027005:intron |
| AGGAATAGCATCCTTGTATC+AGG | 0.295510 | 3.4:+49946186 | None:intergenic |
| TCGATTTGCTTGTTGTGTTC+AGG | 0.306285 | 3.4:+49945213 | None:intergenic |
| TGCAGCCACTTCCTAGTTGT+TGG | 0.322430 | 3.4:+49943000 | None:intergenic |
| TCCCCTGTGCCTGCGCACTA+TGG | 0.322674 | 3.4:-49944744 | MS.gene027005:CDS |
| AGAAAGTAACATTACCATTC+AGG | 0.352299 | 3.4:+49946166 | None:intergenic |
| TTCACAATGTTGGAGGGAAT+CGG | 0.370583 | 3.4:-49942969 | MS.gene027005:CDS |
| GCAGCCACTTCCTAGTTGTT+GGG | 0.391810 | 3.4:+49943001 | None:intergenic |
| CCATTGCTATGGAATTCATT+TGG | 0.393527 | 3.4:-49947694 | MS.gene027005:CDS |
| GATTGTGCTGGAGTGAGGTT+GGG | 0.411334 | 3.4:+49946736 | None:intergenic |
| TCTGTTAGACGATGTGGGCT+TGG | 0.418307 | 3.4:-49943941 | MS.gene027005:CDS |
| TGATTGTGCTGGAGTGAGGT+TGG | 0.420676 | 3.4:+49946735 | None:intergenic |
| ATGAACAACACTTCTTCATT+CGG | 0.420907 | 3.4:-49950397 | MS.gene027005:CDS |
| GTGCCTGCGCACTATGGAGA+TGG | 0.431875 | 3.4:-49944738 | MS.gene027005:CDS |
| GCTGTCTTCCTGACATGTTA+AGG | 0.435901 | 3.4:-49943619 | MS.gene027005:CDS |
| TTGGAAGAAGCGGGAGAAGG+AGG | 0.436211 | 3.4:+49950367 | None:intergenic |
| CCTCCTCCACCTTGCAGTGC+AGG | 0.438234 | 3.4:+49942943 | None:intergenic |
| CACACTCGATTTGATTGTGC+TGG | 0.438779 | 3.4:+49946724 | None:intergenic |
| TACTTGAGGCTTACTAGTCT+TGG | 0.443121 | 3.4:-49945179 | MS.gene027005:CDS |
| TCACAATGTTGGAGGGAATC+GGG | 0.446766 | 3.4:-49942968 | MS.gene027005:CDS |
| CAAATGAATTCCATAGCAAT+GGG | 0.448741 | 3.4:+49947695 | None:intergenic |
| CAATGCTCTTGCCCTGCTCC+AGG | 0.456652 | 3.4:-49946701 | MS.gene027005:intron |
| TACTATTGCAGCACTTCTGC+AGG | 0.460816 | 3.4:-49947671 | MS.gene027005:intron |
| TCTCCATAGTGCGCAGGCAC+AGG | 0.464066 | 3.4:+49944741 | None:intergenic |
| CTGTCTTCCTGACATGTTAA+GGG | 0.464598 | 3.4:-49943618 | MS.gene027005:CDS |
| CCTGCACTGCAAGGTGGAGG+AGG | 0.475249 | 3.4:-49942943 | MS.gene027005:CDS |
| GGAAGCCATCGAGGATGAGT+TGG | 0.478072 | 3.4:+49950348 | None:intergenic |
| AAGATCTAGCCCCATTGCTA+TGG | 0.486924 | 3.4:-49947705 | MS.gene027005:CDS |
| GAGTTGGAAGAAGCGGGAGA+AGG | 0.494679 | 3.4:+49950364 | None:intergenic |
| GTAAGCCTCAAGTACTCAAA+AGG | 0.495117 | 3.4:+49945188 | None:intergenic |
| GTAGCATCCCTTAACATGTC+AGG | 0.500922 | 3.4:+49943611 | None:intergenic |
| AGGTTGGGAGGAGACAAAAC+AGG | 0.502280 | 3.4:+49946751 | None:intergenic |
| TGCCTGCGCACTATGGAGAT+GGG | 0.509168 | 3.4:-49944737 | MS.gene027005:CDS |
| AAACTCATCTAACCTGGAGC+AGG | 0.510559 | 3.4:+49946689 | None:intergenic |
| GTTCCTGCACTGCAAGGTGG+AGG | 0.514765 | 3.4:-49942946 | MS.gene027005:CDS |
| CAACAACTGATTCACAATGT+TGG | 0.517621 | 3.4:-49942979 | MS.gene027005:CDS |
| CCAAATGAATTCCATAGCAA+TGG | 0.523846 | 3.4:+49947694 | None:intergenic |
| AAAGATTCTGTTAGACGATG+TGG | 0.532417 | 3.4:-49943947 | MS.gene027005:CDS |
| TTGCTTGTTGTGTTCAGGAA+CGG | 0.537424 | 3.4:+49945218 | None:intergenic |
| TTCTTCCAACTCATCCTCGA+TGG | 0.538641 | 3.4:-49950353 | MS.gene027005:CDS |
| CTCCATAGTGCGCAGGCACA+GGG | 0.541034 | 3.4:+49944742 | None:intergenic |
| CTTACTAGTCTTGGTGTCAT+CGG | 0.543029 | 3.4:-49945170 | MS.gene027005:CDS |
| CGAGGATGAGTTGGAAGAAG+CGG | 0.546806 | 3.4:+49950357 | None:intergenic |
| GGGGAGTGAGCTCTCTAAAA+CGG | 0.547334 | 3.4:-49944717 | MS.gene027005:intron |
| TAGCATCTCACCCTGATACA+AGG | 0.553761 | 3.4:-49946197 | MS.gene027005:CDS |
| GAGGATGAGTTGGAAGAAGC+GGG | 0.555350 | 3.4:+49950358 | None:intergenic |
| TTCGAGAACGAGTTGTTCAG+AGG | 0.556150 | 3.4:+49950327 | None:intergenic |
| ACAAGGATGCTATTCCTGAA+TGG | 0.556336 | 3.4:-49946180 | MS.gene027005:intron |
| TGCTTCAACAGACGAGATGA+TGG | 0.564466 | 3.4:+49943838 | None:intergenic |
| TCCCCATCTCCATAGTGCGC+AGG | 0.574764 | 3.4:+49944735 | None:intergenic |
| AACTGATTCACAATGTTGGA+GGG | 0.575243 | 3.4:-49942975 | MS.gene027005:CDS |
| CAACTGATTCACAATGTTGG+AGG | 0.581651 | 3.4:-49942976 | MS.gene027005:CDS |
| TTCAGGAGGACCCAACAACT+AGG | 0.584464 | 3.4:-49943011 | MS.gene027005:intron |
| AAGATTCTGTTAGACGATGT+GGG | 0.593200 | 3.4:-49943946 | MS.gene027005:CDS |
| AGGACCCAACAACTAGGAAG+TGG | 0.596471 | 3.4:-49943005 | MS.gene027005:CDS |
| AATCGGGTTCCTGCACTGCA+AGG | 0.603945 | 3.4:-49942952 | MS.gene027005:CDS |
| TGTGCTGGAGTGAGGTTGGG+AGG | 0.609651 | 3.4:+49946739 | None:intergenic |
| TGGTCGAGTTCTTGGAAACA+TGG | 0.623015 | 3.4:-49943881 | MS.gene027005:CDS |
| GATTTGATTGTGCTGGAGTG+AGG | 0.624347 | 3.4:+49946731 | None:intergenic |
| AACTCATCTAACCTGGAGCA+GGG | 0.624665 | 3.4:+49946690 | None:intergenic |
| GGAATAGCATCCTTGTATCA+GGG | 0.625986 | 3.4:+49946187 | None:intergenic |
| TTCGTCTGTCAGATAACCCG+AGG | 0.629082 | 3.4:-49943798 | MS.gene027005:intron |
| GCCTGCGCACTATGGAGATG+GGG | 0.643265 | 3.4:-49944736 | MS.gene027005:CDS |
| CGGGTTCCTGCACTGCAAGG+TGG | 0.654150 | 3.4:-49942949 | MS.gene027005:CDS |
| TGTCATCGGTGCATTAGTGA+AGG | 0.655115 | 3.4:-49945156 | MS.gene027005:intron |
| TCCATAGTGCGCAGGCACAG+GGG | 0.677780 | 3.4:+49944743 | None:intergenic |
| TTGTTCAGAGGAAGCCATCG+AGG | 0.678817 | 3.4:+49950339 | None:intergenic |
| TCACCAGGTTGATGATACTG+AGG | 0.679946 | 3.4:-49944798 | MS.gene027005:intron |
| TCGAGTTCTTGGAAACATGG+TGG | 0.707168 | 3.4:-49943878 | MS.gene027005:CDS |
| GGTTGGGAGGAGACAAAACA+GGG | 0.710997 | 3.4:+49946752 | None:intergenic |
| AGGAACGGATACAAATACAG+AGG | 0.734864 | 3.4:+49945233 | None:intergenic |
| AAATGAATTCCATAGCAATG+GGG | 0.741455 | 3.4:+49947696 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 49942918 | 49950419 | 49942918 | ID=MS.gene027005 |
| chr3.4 | mRNA | 49942918 | 49950419 | 49942918 | ID=MS.gene027005.t1;Parent=MS.gene027005 |
| chr3.4 | exon | 49950279 | 49950419 | 49950279 | ID=MS.gene027005.t1.exon1;Parent=MS.gene027005.t1 |
| chr3.4 | CDS | 49950279 | 49950419 | 49950279 | ID=cds.MS.gene027005.t1;Parent=MS.gene027005.t1 |
| chr3.4 | exon | 49947672 | 49947743 | 49947672 | ID=MS.gene027005.t1.exon2;Parent=MS.gene027005.t1 |
| chr3.4 | CDS | 49947672 | 49947743 | 49947672 | ID=cds.MS.gene027005.t1;Parent=MS.gene027005.t1 |
| chr3.4 | exon | 49946702 | 49946791 | 49946702 | ID=MS.gene027005.t1.exon3;Parent=MS.gene027005.t1 |
| chr3.4 | CDS | 49946702 | 49946791 | 49946702 | ID=cds.MS.gene027005.t1;Parent=MS.gene027005.t1 |
| chr3.4 | exon | 49946181 | 49946223 | 49946181 | ID=MS.gene027005.t1.exon4;Parent=MS.gene027005.t1 |
| chr3.4 | CDS | 49946181 | 49946223 | 49946181 | ID=cds.MS.gene027005.t1;Parent=MS.gene027005.t1 |
| chr3.4 | exon | 49945157 | 49945263 | 49945157 | ID=MS.gene027005.t1.exon5;Parent=MS.gene027005.t1 |
| chr3.4 | CDS | 49945157 | 49945263 | 49945157 | ID=cds.MS.gene027005.t1;Parent=MS.gene027005.t1 |
| chr3.4 | exon | 49944718 | 49944813 | 49944718 | ID=MS.gene027005.t1.exon6;Parent=MS.gene027005.t1 |
| chr3.4 | CDS | 49944718 | 49944813 | 49944718 | ID=cds.MS.gene027005.t1;Parent=MS.gene027005.t1 |
| chr3.4 | exon | 49943799 | 49943989 | 49943799 | ID=MS.gene027005.t1.exon7;Parent=MS.gene027005.t1 |
| chr3.4 | CDS | 49943799 | 49943989 | 49943799 | ID=cds.MS.gene027005.t1;Parent=MS.gene027005.t1 |
| chr3.4 | exon | 49943592 | 49943661 | 49943592 | ID=MS.gene027005.t1.exon8;Parent=MS.gene027005.t1 |
| chr3.4 | CDS | 49943592 | 49943661 | 49943592 | ID=cds.MS.gene027005.t1;Parent=MS.gene027005.t1 |
| chr3.4 | exon | 49942918 | 49943028 | 49942918 | ID=MS.gene027005.t1.exon9;Parent=MS.gene027005.t1 |
| chr3.4 | CDS | 49942918 | 49943028 | 49942918 | ID=cds.MS.gene027005.t1;Parent=MS.gene027005.t1 |
| Gene Sequence |
| Protein sequence |
