Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027105.t1 | QCE00476.1 | 34.7 | 95 | 58 | 3 | 21 | 114 | 204 | 295 | 8.50E-06 | 60.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027105.t1 | A0A4D6MGG0 | 34.7 | 95 | 58 | 3 | 21 | 114 | 204 | 295 | 6.2e-06 | 60.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene027105.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCTCCAACGGCATTTCTC+AGG | 0.329072 | 3.4:-48552680 | MS.gene027105:CDS |
| GGTGGGAATGTGGCTAATAA+TGG | 0.330968 | 3.4:-48552985 | MS.gene027105:CDS |
| ACTCGTGCAACAAATCTTCT+TGG | 0.342234 | 3.4:+48552795 | None:intergenic |
| TGGCAGTTGGGAAAAGTGTT+AGG | 0.395161 | 3.4:-48552623 | MS.gene027105:CDS |
| CGGAGAACCGGCTGGCAGTT+GGG | 0.421662 | 3.4:-48552635 | MS.gene027105:CDS |
| GATTTGTTGCACGAGTGTTT+CGG | 0.452697 | 3.4:-48552788 | MS.gene027105:CDS |
| AGAGATTTGTTACGGAGAAC+CGG | 0.452877 | 3.4:-48552647 | MS.gene027105:CDS |
| TCCTCCAACGGCATTTCTCA+GGG | 0.453191 | 3.4:-48552679 | MS.gene027105:CDS |
| GTGGGAATGTGGCTAATAAT+GGG | 0.465866 | 3.4:-48552984 | MS.gene027105:CDS |
| CGGGCTTTGACTTTCCGTGC+AGG | 0.468251 | 3.4:-48552728 | MS.gene027105:CDS |
| ACTCATATGTTATGTATGCT+AGG | 0.478622 | 3.4:-48552581 | MS.gene027105:CDS |
| ACGGAGAACCGGCTGGCAGT+TGG | 0.492128 | 3.4:-48552636 | MS.gene027105:CDS |
| CAACGGCATTTCTCAGGGGC+GGG | 0.504053 | 3.4:-48552674 | MS.gene027105:CDS |
| CGGCATTTCTCAGGGGCGGG+TGG | 0.507125 | 3.4:-48552671 | MS.gene027105:CDS |
| TGTGGCTAATAATGGGAAAG+TGG | 0.520792 | 3.4:-48552977 | MS.gene027105:CDS |
| CCCCTGAGAAATGCCGTTGG+AGG | 0.522638 | 3.4:+48552678 | None:intergenic |
| GTATGCTAGGGAAGCTCGGA+TGG | 0.537149 | 3.4:-48552568 | MS.gene027105:CDS |
| CTCATATGTTATGTATGCTA+GGG | 0.545482 | 3.4:-48552580 | MS.gene027105:CDS |
| CCAACGGCATTTCTCAGGGG+CGG | 0.547897 | 3.4:-48552675 | MS.gene027105:CDS |
| ATTTGTTACGGAGAACCGGC+TGG | 0.553165 | 3.4:-48552643 | MS.gene027105:CDS |
| CATGGTGAAGACTAGCAATC+CGG | 0.556931 | 3.4:-48552926 | MS.gene027105:intron |
| TCCTTCCACTTGATCCTGCA+CGG | 0.565208 | 3.4:+48552714 | None:intergenic |
| TTATGTATGCTAGGGAAGCT+CGG | 0.572454 | 3.4:-48552572 | MS.gene027105:CDS |
| GGTGAAAACATGGGTGCAGG+TGG | 0.578164 | 3.4:-48553003 | MS.gene027105:CDS |
| ACTTTCCGTGCAGGATCAAG+TGG | 0.580280 | 3.4:-48552719 | MS.gene027105:CDS |
| CATGGGTGCAGGTGGGAATG+TGG | 0.582106 | 3.4:-48552995 | MS.gene027105:CDS |
| GGCATCTGTAAGTAGGATGG+TGG | 0.582230 | 3.4:+48552752 | None:intergenic |
| CATCCTACTTACAGATGCCA+CGG | 0.586192 | 3.4:-48552748 | MS.gene027105:CDS |
| CGGGTGGAAGAGATTTGTTA+CGG | 0.590170 | 3.4:-48552655 | MS.gene027105:CDS |
| CGTGGCATCTGTAAGTAGGA+TGG | 0.594922 | 3.4:+48552749 | None:intergenic |
| CCGCCCCTGAGAAATGCCGT+TGG | 0.596478 | 3.4:+48552675 | None:intergenic |
| GGGTAACAATGGTGAAAACA+TGG | 0.597431 | 3.4:-48553013 | MS.gene027105:CDS |
| ATCCTACTTACAGATGCCAC+GGG | 0.598598 | 3.4:-48552747 | MS.gene027105:CDS |
| GTGCAACAAATCTTCTTGGT+AGG | 0.604465 | 3.4:+48552799 | None:intergenic |
| GAAATGCCGTTGGAGGAAGA+TGG | 0.605964 | 3.4:+48552685 | None:intergenic |
| AGCCCGTGGCATCTGTAAGT+AGG | 0.608677 | 3.4:+48552745 | None:intergenic |
| TGTCAGACTTGCAGAATACA+TGG | 0.612787 | 3.4:-48552944 | MS.gene027105:CDS |
| AATGGTGAAAACATGGGTGC+AGG | 0.619307 | 3.4:-48553006 | MS.gene027105:CDS |
| CCTCCAACGGCATTTCTCAG+GGG | 0.622449 | 3.4:-48552678 | MS.gene027105:CDS |
| GCATCTGTAAGTAGGATGGT+GGG | 0.632206 | 3.4:+48552753 | None:intergenic |
| TCCGTGCAGGATCAAGTGGA+AGG | 0.637315 | 3.4:-48552715 | MS.gene027105:CDS |
| CAGACGCCATCTTCCTCCAA+CGG | 0.642200 | 3.4:-48552691 | MS.gene027105:CDS |
| GCACGGAAAGTCAAAGCCCG+TGG | 0.648025 | 3.4:+48552731 | None:intergenic |
| GCTGTTGCCATGGGTAACAA+TGG | 0.655928 | 3.4:-48553024 | None:intergenic |
| GTGAAAACATGGGTGCAGGT+GGG | 0.671071 | 3.4:-48553002 | MS.gene027105:CDS |
| TCTGTAAGTAGGATGGTGGG+AGG | 0.702880 | 3.4:+48552756 | None:intergenic |
| GGTAACAATGGTGAAAACAT+GGG | 0.742892 | 3.4:-48553012 | MS.gene027105:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTACTGTTATATAATTTT+TGG | - | chr3.4:48552705-48552724 | MS.gene027105:CDS | 10.0% |
| !!! | AAATGATAACAATTTAAAAC+AGG | + | chr3.4:48552746-48552765 | None:intergenic | 15.0% |
| ACTCATATGTTATGTATGCT+AGG | - | chr3.4:48552994-48553013 | MS.gene027105:CDS | 30.0% | |
| CTCATATGTTATGTATGCTA+GGG | - | chr3.4:48552995-48553014 | MS.gene027105:CDS | 30.0% | |
| GGTAACAATGGTGAAAACAT+GGG | - | chr3.4:48552563-48552582 | MS.gene027105:CDS | 35.0% | |
| ACTCGTGCAACAAATCTTCT+TGG | + | chr3.4:48552783-48552802 | None:intergenic | 40.0% | |
| CAGTTATGCAATTCACCTAC+CGG | + | chr3.4:48552671-48552690 | None:intergenic | 40.0% | |
| GGGTAACAATGGTGAAAACA+TGG | - | chr3.4:48552562-48552581 | MS.gene027105:CDS | 40.0% | |
| GTGCAACAAATCTTCTTGGT+AGG | + | chr3.4:48552779-48552798 | None:intergenic | 40.0% | |
| GTGGGAATGTGGCTAATAAT+GGG | - | chr3.4:48552591-48552610 | MS.gene027105:CDS | 40.0% | |
| TGTCAGACTTGCAGAATACA+TGG | - | chr3.4:48552631-48552650 | MS.gene027105:CDS | 40.0% | |
| TTATGTATGCTAGGGAAGCT+CGG | - | chr3.4:48553003-48553022 | MS.gene027105:CDS | 40.0% | |
| ! | TGTGGCTAATAATGGGAAAG+TGG | - | chr3.4:48552598-48552617 | MS.gene027105:CDS | 40.0% |
| !! | AGAGATTTGTTACGGAGAAC+CGG | - | chr3.4:48552928-48552947 | MS.gene027105:CDS | 40.0% |
| !! | GATTTGTTGCACGAGTGTTT+CGG | - | chr3.4:48552787-48552806 | MS.gene027105:CDS | 40.0% |
| AATGGTGAAAACATGGGTGC+AGG | - | chr3.4:48552569-48552588 | MS.gene027105:CDS | 45.0% | |
| ATCCTACTTACAGATGCCAC+GGG | - | chr3.4:48552828-48552847 | MS.gene027105:intron | 45.0% | |
| CATCCTACTTACAGATGCCA+CGG | - | chr3.4:48552827-48552846 | MS.gene027105:intron | 45.0% | |
| CATGGTGAAGACTAGCAATC+CGG | - | chr3.4:48552649-48552668 | MS.gene027105:CDS | 45.0% | |
| GCATCTGTAAGTAGGATGGT+GGG | + | chr3.4:48552825-48552844 | None:intergenic | 45.0% | |
| GGTGGGAATGTGGCTAATAA+TGG | - | chr3.4:48552590-48552609 | MS.gene027105:CDS | 45.0% | |
| !! | CGGGTGGAAGAGATTTGTTA+CGG | - | chr3.4:48552920-48552939 | MS.gene027105:intron | 45.0% |
| !! | TGGCAGTTGGGAAAAGTGTT+AGG | - | chr3.4:48552952-48552971 | MS.gene027105:CDS | 45.0% |
| ACTTTCCGTGCAGGATCAAG+TGG | - | chr3.4:48552856-48552875 | MS.gene027105:intron | 50.0% | |
| CGTGGCATCTGTAAGTAGGA+TGG | + | chr3.4:48552829-48552848 | None:intergenic | 50.0% | |
| GAAATGCCGTTGGAGGAAGA+TGG | + | chr3.4:48552893-48552912 | None:intergenic | 50.0% | |
| GGCATCTGTAAGTAGGATGG+TGG | + | chr3.4:48552826-48552845 | None:intergenic | 50.0% | |
| GTGAAAACATGGGTGCAGGT+GGG | - | chr3.4:48552573-48552592 | MS.gene027105:CDS | 50.0% | |
| GTGAAGACTAGCAATCCGGT+AGG | - | chr3.4:48552653-48552672 | MS.gene027105:CDS | 50.0% | |
| TCCTCCAACGGCATTTCTCA+GGG | - | chr3.4:48552896-48552915 | MS.gene027105:intron | 50.0% | |
| TCCTTCCACTTGATCCTGCA+CGG | + | chr3.4:48552864-48552883 | None:intergenic | 50.0% | |
| TTCCTCCAACGGCATTTCTC+AGG | - | chr3.4:48552895-48552914 | MS.gene027105:intron | 50.0% | |
| ! | ATTTGTTACGGAGAACCGGC+TGG | - | chr3.4:48552932-48552951 | MS.gene027105:CDS | 50.0% |
| !! | TCTGTAAGTAGGATGGTGGG+AGG | + | chr3.4:48552822-48552841 | None:intergenic | 50.0% |
| AGCCCGTGGCATCTGTAAGT+AGG | + | chr3.4:48552833-48552852 | None:intergenic | 55.0% | |
| CAGACGCCATCTTCCTCCAA+CGG | - | chr3.4:48552884-48552903 | MS.gene027105:intron | 55.0% | |
| CCTCCAACGGCATTTCTCAG+GGG | - | chr3.4:48552897-48552916 | MS.gene027105:intron | 55.0% | |
| GGTGAAAACATGGGTGCAGG+TGG | - | chr3.4:48552572-48552591 | MS.gene027105:CDS | 55.0% | |
| GTATGCTAGGGAAGCTCGGA+TGG | - | chr3.4:48553007-48553026 | MS.gene027105:CDS | 55.0% | |
| TCCGTGCAGGATCAAGTGGA+AGG | - | chr3.4:48552860-48552879 | MS.gene027105:intron | 55.0% | |
| ! | CACTTTTCCCAACTGCCAGC+CGG | + | chr3.4:48552950-48552969 | None:intergenic | 55.0% |
| CAACGGCATTTCTCAGGGGC+GGG | - | chr3.4:48552901-48552920 | MS.gene027105:intron | 60.0% | |
| CATGGGTGCAGGTGGGAATG+TGG | - | chr3.4:48552580-48552599 | MS.gene027105:CDS | 60.0% | |
| CCAACGGCATTTCTCAGGGG+CGG | - | chr3.4:48552900-48552919 | MS.gene027105:intron | 60.0% | |
| CCCCTGAGAAATGCCGTTGG+AGG | + | chr3.4:48552900-48552919 | None:intergenic | 60.0% | |
| GCACGGAAAGTCAAAGCCCG+TGG | + | chr3.4:48552847-48552866 | None:intergenic | 60.0% | |
| ! | CGGGCTTTGACTTTCCGTGC+AGG | - | chr3.4:48552847-48552866 | MS.gene027105:intron | 60.0% |
| ACGGAGAACCGGCTGGCAGT+TGG | - | chr3.4:48552939-48552958 | MS.gene027105:CDS | 65.0% | |
| CCGCCCCTGAGAAATGCCGT+TGG | + | chr3.4:48552903-48552922 | None:intergenic | 65.0% | |
| CGGAGAACCGGCTGGCAGTT+GGG | - | chr3.4:48552940-48552959 | MS.gene027105:CDS | 65.0% | |
| CGGCATTTCTCAGGGGCGGG+TGG | - | chr3.4:48552904-48552923 | MS.gene027105:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 48552560 | 48553037 | 48552560 | ID=MS.gene027105 |
| chr3.4 | mRNA | 48552560 | 48553037 | 48552560 | ID=MS.gene027105.t1;Parent=MS.gene027105 |
| chr3.4 | exon | 48552927 | 48553037 | 48552927 | ID=MS.gene027105.t1.exon1;Parent=MS.gene027105.t1 |
| chr3.4 | CDS | 48552927 | 48553037 | 48552927 | ID=cds.MS.gene027105.t1;Parent=MS.gene027105.t1 |
| chr3.4 | exon | 48552560 | 48552823 | 48552560 | ID=MS.gene027105.t1.exon2;Parent=MS.gene027105.t1 |
| chr3.4 | CDS | 48552560 | 48552823 | 48552560 | ID=cds.MS.gene027105.t1;Parent=MS.gene027105.t1 |
| Gene Sequence |
| Protein sequence |
