AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027111

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 103 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGAACGTGGCTAATTTC+TGG 0.218063 3.4:+48522706 MS.gene027111:CDS
AATCTCCATCGGTATATTTC+AGG 0.235698 3.4:+48523128 MS.gene027111:CDS
AGAGCATAGAGTTTGGTTTC+AGG 0.263168 3.4:+48522116 MS.gene027111:CDS
GGCGGTGGTTGCTTCTATTC+AGG 0.264711 3.4:+48522183 MS.gene027111:CDS
TGTTCTTCCACCACTGGTAC+TGG 0.276664 3.4:-48522651 None:intergenic
GGTATATTTCAGGGGCGGTT+GGG 0.311650 3.4:+48523138 MS.gene027111:CDS
GAGGAACCTGCTTTCGATAT+TGG 0.318864 3.4:+48522738 MS.gene027111:CDS
GAGTTGTGCGCATCATTAAT+TGG 0.323647 3.4:-48522585 None:intergenic
AAATTTGCTCGGCGAGCATT+TGG 0.329497 3.4:+48523019 MS.gene027111:CDS
CGGTATATTTCAGGGGCGGT+TGG 0.352390 3.4:+48523137 MS.gene027111:CDS
GTCAAATAGAGCATAGAGTT+TGG 0.371033 3.4:+48522109 MS.gene027111:CDS
TTAGAGCACCATACTTGGTT+TGG 0.380253 3.4:-48521449 None:intergenic
TTAAAACATGTGTCTCCAAC+TGG 0.380379 3.4:-48522830 None:intergenic
CGATGTTGAATACCCTCCTT+TGG 0.387542 3.4:-48521418 None:intergenic
GATCATTATGAGCAACCATT+AGG 0.389483 3.4:+48522222 MS.gene027111:CDS
TAGAGCACCATACTTGGTTT+GGG 0.392335 3.4:-48521448 None:intergenic
CATTCCTGATGATGGTGATA+TGG 0.395267 3.4:+48522614 MS.gene027111:CDS
GCACGTGAGGCAGGTCGTTA+TGG 0.400336 3.4:+48523240 MS.gene027111:CDS
GCTATGGCAGCGGCAGTTTG+TGG 0.412204 3.4:-48522678 None:intergenic
TCAGAACGTGGCTAATTTCT+GGG 0.416922 3.4:+48522707 MS.gene027111:CDS
GTATATTTCAGGGGCGGTTG+GGG 0.419452 3.4:+48523139 MS.gene027111:CDS
AATCGTAGGTTTAGACCTAA+TGG 0.433227 3.4:-48522237 None:intergenic
AATTTGCTCGGCGAGCATTT+GGG 0.441935 3.4:+48523020 MS.gene027111:CDS
CATCATTAATTGGATTTGAC+GGG 0.443530 3.4:-48522575 None:intergenic
GCCTCACGTGCAGGACAGTT+GGG 0.444919 3.4:-48523228 None:intergenic
CGTTAGAGAGAACAAATTTG+TGG 0.450427 3.4:+48523168 MS.gene027111:CDS
TCCACCACTGGTACTGGGTC+AGG 0.460719 3.4:-48522645 None:intergenic
AAACAGCCCGTTATCGTCAT+AGG 0.462077 3.4:-48522262 None:intergenic
GTTCCACCGGGTTTCGAAGT+AGG 0.463343 3.4:+48522549 MS.gene027111:CDS
GATACCAGAGGGTCTAGGTT+TGG 0.463429 3.4:+48522138 MS.gene027111:CDS
TCATACCACTTAATATCGAT+TGG 0.463682 3.4:-48523094 None:intergenic
GTTCTTCCACCACTGGTACT+GGG 0.464509 3.4:-48522650 None:intergenic
GCATCATTAATTGGATTTGA+CGG 0.475292 3.4:-48522576 None:intergenic
TGCCTCACGTGCAGGACAGT+TGG 0.476556 3.4:-48523229 None:intergenic
GGCCGCTATGGATGTCGAAA+TGG 0.484746 3.4:+48522773 MS.gene027111:CDS
TCATCCATGTCAAGAGTGAA+TGG 0.486104 3.4:-48522294 None:intergenic
ATCTCCATCGGTATATTTCA+GGG 0.486502 3.4:+48523129 MS.gene027111:CDS
CTCATCTCGGATCGTGATGA+AGG 0.492063 3.4:-48523056 None:intergenic
GATTGACCTATGACGATAAC+GGG 0.493046 3.4:+48522256 MS.gene027111:CDS
TCTCCTCCCATTCCTGATGA+TGG 0.497542 3.4:+48522606 MS.gene027111:CDS
TCTAGGTTTGGAGTATGTTA+CGG 0.497585 3.4:+48522150 MS.gene027111:CDS
CGATTGACCTATGACGATAA+CGG 0.503122 3.4:+48522255 MS.gene027111:CDS
AACTGTCCTGCACGTGAGGC+AGG 0.503776 3.4:+48523231 MS.gene027111:CDS
TTCAGGATACCAGAGGGTCT+AGG 0.513988 3.4:+48522133 MS.gene027111:CDS
TACTCCAAACCTAGACCCTC+TGG 0.515790 3.4:-48522142 None:intergenic
CATATCACCATCATCAGGAA+TGG 0.516230 3.4:-48522613 None:intergenic
ATTAGCCACGTTCTGAATCT+CGG 0.517907 3.4:-48522700 None:intergenic
AGTATCCAATCGATATTAAG+TGG 0.520888 3.4:+48523089 MS.gene027111:CDS
TTTCGATATTGGTGATAATG+AGG 0.526872 3.4:+48522749 MS.gene027111:CDS
TTTAACGCTTGCTGCGTACA+TGG 0.531915 3.4:+48522848 MS.gene027111:CDS
AGCTTACGGCTCGTGTCCCA+TGG 0.534486 3.4:+48522064 MS.gene027111:intron
TAACGACCTGCCTCACGTGC+AGG 0.537930 3.4:-48523237 None:intergenic
ACTACCATATCACCATCATC+AGG 0.542080 3.4:-48522618 None:intergenic
ACAAATTTGTGGAAGGCACA+AGG 0.542209 3.4:+48523179 MS.gene027111:CDS
TGATAATGAGGAGGCCGCTA+TGG 0.542930 3.4:+48522761 MS.gene027111:CDS
CTGAATCTCGGCTATGGCAG+CGG 0.548304 3.4:-48522688 None:intergenic
TCTCGGATCGTGATGAAGGT+TGG 0.548455 3.4:-48523052 None:intergenic
TAAAACATGTGTCTCCAACT+GGG 0.552768 3.4:-48522829 None:intergenic
ATATCACCATCATCAGGAAT+GGG 0.554219 3.4:-48522612 None:intergenic
TTGTACCAGCAAAATTTGCT+CGG 0.557897 3.4:+48523008 MS.gene027111:CDS
GGCAGGTCGTTATGGTGTAA+AGG 0.566786 3.4:+48523248 MS.gene027111:CDS
ATTCACTCTTGACATGGATG+AGG 0.568212 3.4:+48522296 MS.gene027111:CDS
CACGTTCTGAATCTCGGCTA+TGG 0.569669 3.4:-48522694 None:intergenic
GCTTCCATTCACTCTTGACA+TGG 0.570881 3.4:+48522290 MS.gene027111:CDS
GTATGTTACGGAGAGATCAA+TGG 0.580224 3.4:+48522162 MS.gene027111:CDS
TGACATGCAACCTTGAGCTA+TGG 0.582304 3.4:-48522347 None:intergenic
GTTGCTCATAATGATCAAGC+AGG 0.585077 3.4:-48522215 None:intergenic
TTATCACCAATATCGAAAGC+AGG 0.592587 3.4:-48522744 None:intergenic
ACCCAACTGTCCTGCACGTG+AGG 0.595873 3.4:+48523227 MS.gene027111:CDS
AGAGAGAACAAATTTGTGGA+AGG 0.598566 3.4:+48523172 MS.gene027111:CDS
CCACCGGGTTTCGAAGTAGG+AGG 0.598715 3.4:+48522552 MS.gene027111:CDS
TGCACATGGCAATCCCCAGT+TGG 0.598828 3.4:+48522815 MS.gene027111:CDS
AAAACATGTGTCTCCAACTG+GGG 0.599659 3.4:-48522828 None:intergenic
CAAATTTGTGGAAGGCACAA+GGG 0.602049 3.4:+48523180 MS.gene027111:CDS
AGGTAACTAATCGTCAATTG+AGG 0.606077 3.4:+48522316 MS.gene027111:CDS
TGTTCCTGACCCAGTACCAG+TGG 0.613436 3.4:+48522641 MS.gene027111:CDS
TATACCGATGGAGATTTATG+CGG 0.614591 3.4:-48523121 None:intergenic
CCATCGGTATATTTCAGGGG+CGG 0.616182 3.4:+48523133 MS.gene027111:CDS
TACGGAGAGATCAATGGCGG+TGG 0.619550 3.4:+48522168 MS.gene027111:CDS
TATCGTCATAGGTCAATCGT+AGG 0.623984 3.4:-48522251 None:intergenic
ACTCACGGTGGAACTCATCT+CGG 0.624872 3.4:-48523069 None:intergenic
CGATATTGGTGATAATGAGG+AGG 0.625464 3.4:+48522752 MS.gene027111:CDS
TCCTGACCCAGTACCAGTGG+TGG 0.629390 3.4:+48522644 MS.gene027111:CDS
TAATATCGATTGGATACTCA+CGG 0.630661 3.4:-48523084 None:intergenic
GTTTGGTTTCAGGATACCAG+AGG 0.643279 3.4:+48522126 MS.gene027111:CDS
TTTGGTTTCAGGATACCAGA+GGG 0.645318 3.4:+48522127 MS.gene027111:CDS
TCACCATCATCAGGAATGGG+AGG 0.646579 3.4:-48522609 None:intergenic
TAATTTCTGGGATGATGATG+AGG 0.648095 3.4:+48522719 MS.gene027111:CDS
CATAGCCGAGATTCAGAACG+TGG 0.650255 3.4:+48522695 MS.gene027111:CDS
GGAACTATGACCATAGCTCA+AGG 0.651950 3.4:+48522337 MS.gene027111:CDS
TATGGATGTCGAAATGGATG+TGG 0.654650 3.4:+48522779 MS.gene027111:CDS
CCGCCCCTGAAATATACCGA+TGG 0.663842 3.4:-48523133 None:intergenic
TCTCCATCGGTATATTTCAG+GGG 0.665166 3.4:+48523130 MS.gene027111:CDS
AAAACCGCATAAATCTCCAT+CGG 0.665372 3.4:+48523117 MS.gene027111:CDS
GGGCCTCCTACTTCGAAACC+CGG 0.671468 3.4:-48522555 None:intergenic
CATGGTGAAATGCAGCAACA+TGG 0.687154 3.4:+48522866 MS.gene027111:CDS
AATGGATGTGGTAGATGCTG+AGG 0.688566 3.4:+48522791 MS.gene027111:CDS
CCTCCTACTTCGAAACCCGG+TGG 0.702465 3.4:-48522552 None:intergenic
ATCCATTTCGACATCCATAG+CGG 0.708971 3.4:-48522775 None:intergenic
TGTTACGGAGAGATCAATGG+CGG 0.735510 3.4:+48522165 MS.gene027111:CDS
GTAGATGCTGAGGATGCACA+TGG 0.739903 3.4:+48522801 MS.gene027111:CDS
AAGGAGGGTATTCAACATCG+CGG 0.764822 3.4:+48521421 MS.gene027111:CDS
TATCGATTGGATACTCACGG+TGG 0.816467 3.4:-48523081 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTAAGAAGAAAAAAAAA+TGG - chr3.4:48521637-48521656 None:intergenic 10.0%
!!! TCATAGATTTTCAATACTTT+TGG + chr3.4:48522003-48522022 MS.gene027111:intron 20.0%
! ACACAACAAAATACAAAGAA+TGG - chr3.4:48521853-48521872 None:intergenic 25.0%
! CTTTATACAGTTATCTATTG+TGG + chr3.4:48522928-48522947 MS.gene027111:intron 25.0%
! TATTGAAAATCTATGAGCAA+AGG - chr3.4:48521999-48522018 None:intergenic 25.0%
!!! TATTTTTACGAACGTTTCAA+AGG + chr3.4:48522087-48522106 MS.gene027111:CDS 25.0%
AAACGTTCGTAAAAATACCA+TGG - chr3.4:48522084-48522103 None:intergenic 30.0%
AACGTTCGTAAAAATACCAT+GGG - chr3.4:48522083-48522102 None:intergenic 30.0%
AGTATCCAATCGATATTAAG+TGG + chr3.4:48523089-48523108 MS.gene027111:CDS 30.0%
ATAGATAACTGTATAAAGCG+TGG - chr3.4:48522926-48522945 None:intergenic 30.0%
CATCATTAATTGGATTTGAC+GGG - chr3.4:48522578-48522597 None:intergenic 30.0%
CCTTCAAAAAAGAGAAAAAC+TGG - chr3.4:48521898-48521917 None:intergenic 30.0%
GCATCATTAATTGGATTTGA+CGG - chr3.4:48522579-48522598 None:intergenic 30.0%
GTTGTGTTGTGTCAAATAAA+TGG + chr3.4:48521866-48521885 MS.gene027111:intron 30.0%
TACAGTTATCTATTGTGGTT+TGG + chr3.4:48522933-48522952 MS.gene027111:intron 30.0%
TCATACCACTTAATATCGAT+TGG - chr3.4:48523097-48523116 None:intergenic 30.0%
TGTTTGTATTCTGTAGCTTA+CGG + chr3.4:48522050-48522069 MS.gene027111:intron 30.0%
! CAGCAGCATTAATTTGAAAT+TGG - chr3.4:48522458-48522477 None:intergenic 30.0%
! TAATATCGATTGGATACTCA+CGG - chr3.4:48523087-48523106 None:intergenic 30.0%
! TTTCGATATTGGTGATAATG+AGG + chr3.4:48522749-48522768 MS.gene027111:CDS 30.0%
!!! CCAGTTTTTCTCTTTTTTGA+AGG + chr3.4:48521895-48521914 MS.gene027111:intron 30.0%
AAAACCGCATAAATCTCCAT+CGG + chr3.4:48523117-48523136 MS.gene027111:CDS 35.0%
AATCGTAGGTTTAGACCTAA+TGG - chr3.4:48522240-48522259 None:intergenic 35.0%
AATCTCCATCGGTATATTTC+AGG + chr3.4:48523128-48523147 MS.gene027111:CDS 35.0%
AATTAATGCTGCTGCTATTG+TGG + chr3.4:48522464-48522483 MS.gene027111:intron 35.0%
AGAGAGAACAAATTTGTGGA+AGG + chr3.4:48523172-48523191 MS.gene027111:CDS 35.0%
AGGTAACTAATCGTCAATTG+AGG + chr3.4:48522316-48522335 MS.gene027111:CDS 35.0%
ATATCACCATCATCAGGAAT+GGG - chr3.4:48522615-48522634 None:intergenic 35.0%
ATCTCCATCGGTATATTTCA+GGG + chr3.4:48523129-48523148 MS.gene027111:CDS 35.0%
ATTAATGCTGCTGCTATTGT+GGG + chr3.4:48522465-48522484 MS.gene027111:intron 35.0%
CGTTAGAGAGAACAAATTTG+TGG + chr3.4:48523168-48523187 MS.gene027111:CDS 35.0%
GATCATTATGAGCAACCATT+AGG + chr3.4:48522222-48522241 MS.gene027111:CDS 35.0%
GTCAAATAGAGCATAGAGTT+TGG + chr3.4:48522109-48522128 MS.gene027111:CDS 35.0%
TAAAACATGTGTCTCCAACT+GGG - chr3.4:48522832-48522851 None:intergenic 35.0%
TATACCGATGGAGATTTATG+CGG - chr3.4:48523124-48523143 None:intergenic 35.0%
TTAAAACATGTGTCTCCAAC+TGG - chr3.4:48522833-48522852 None:intergenic 35.0%
TTATCACCAATATCGAAAGC+AGG - chr3.4:48522747-48522766 None:intergenic 35.0%
! ACAAAGAATGGAAGTGTATG+AGG - chr3.4:48521841-48521860 None:intergenic 35.0%
! ACCAGATATGGAAGTGTATT+GGG - chr3.4:48521539-48521558 None:intergenic 35.0%
! AGCATCGCATTTTGAATCAT+TGG - chr3.4:48521485-48521504 None:intergenic 35.0%
! CAAAGTATCTAAATGTACGC+AGG - chr3.4:48522380-48522399 None:intergenic 35.0%
! TCTAGGTTTGGAGTATGTTA+CGG + chr3.4:48522150-48522169 MS.gene027111:CDS 35.0%
! TTGTACCAGCAAAATTTGCT+CGG + chr3.4:48523008-48523027 MS.gene027111:CDS 35.0%
!! TAATTTCTGGGATGATGATG+AGG + chr3.4:48522719-48522738 MS.gene027111:CDS 35.0%
AAAACATGTGTCTCCAACTG+GGG - chr3.4:48522831-48522850 None:intergenic 40.0%
AAAATCTATGAGCAAAGGCG+AGG - chr3.4:48521994-48522013 None:intergenic 40.0%
AAATCTATGAGCAAAGGCGA+GGG - chr3.4:48521993-48522012 None:intergenic 40.0%
AATGCAACATCCACCAGATA+TGG - chr3.4:48521551-48521570 None:intergenic 40.0%
ACTACCATATCACCATCATC+AGG - chr3.4:48522621-48522640 None:intergenic 40.0%
ATCCATTTCGACATCCATAG+CGG - chr3.4:48522778-48522797 None:intergenic 40.0%
ATTCACTCTTGACATGGATG+AGG + chr3.4:48522296-48522315 MS.gene027111:CDS 40.0%
CAATACACTTCCATATCTGG+TGG + chr3.4:48521538-48521557 MS.gene027111:intron 40.0%
CATATCACCATCATCAGGAA+TGG - chr3.4:48522616-48522635 None:intergenic 40.0%
CGATTGACCTATGACGATAA+CGG + chr3.4:48522255-48522274 MS.gene027111:CDS 40.0%
GAGTTGTGCGCATCATTAAT+TGG - chr3.4:48522588-48522607 None:intergenic 40.0%
GATTGACCTATGACGATAAC+GGG + chr3.4:48522256-48522275 MS.gene027111:CDS 40.0%
GTATGTTACGGAGAGATCAA+TGG + chr3.4:48522162-48522181 MS.gene027111:CDS 40.0%
GTTGCTCATAATGATCAAGC+AGG - chr3.4:48522218-48522237 None:intergenic 40.0%
TATCGTCATAGGTCAATCGT+AGG - chr3.4:48522254-48522273 None:intergenic 40.0%
TATGGATGTCGAAATGGATG+TGG + chr3.4:48522779-48522798 MS.gene027111:CDS 40.0%
TCAGAACGTGGCTAATTTCT+GGG + chr3.4:48522707-48522726 MS.gene027111:CDS 40.0%
TCATCCATGTCAAGAGTGAA+TGG - chr3.4:48522297-48522316 None:intergenic 40.0%
TCTCCATCGGTATATTTCAG+GGG + chr3.4:48523130-48523149 MS.gene027111:CDS 40.0%
TTCAGAACGTGGCTAATTTC+TGG + chr3.4:48522706-48522725 MS.gene027111:CDS 40.0%
! ACAAATTTGTGGAAGGCACA+AGG + chr3.4:48523179-48523198 MS.gene027111:CDS 40.0%
! AGAGCATAGAGTTTGGTTTC+AGG + chr3.4:48522116-48522135 MS.gene027111:CDS 40.0%
! ATTAGCCACGTTCTGAATCT+CGG - chr3.4:48522703-48522722 None:intergenic 40.0%
! CAAATTTGTGGAAGGCACAA+GGG + chr3.4:48523180-48523199 MS.gene027111:CDS 40.0%
! CACCAGATATGGAAGTGTAT+TGG - chr3.4:48521540-48521559 None:intergenic 40.0%
! CCAGATATGGAAGTGTATTG+GGG - chr3.4:48521538-48521557 None:intergenic 40.0%
! CGATATTGGTGATAATGAGG+AGG + chr3.4:48522752-48522771 MS.gene027111:CDS 40.0%
! GAAGATTAGAGCACCATACT+TGG - chr3.4:48521457-48521476 None:intergenic 40.0%
! TAGAGCACCATACTTGGTTT+GGG - chr3.4:48521451-48521470 None:intergenic 40.0%
! TTAGAGCACCATACTTGGTT+TGG - chr3.4:48521452-48521471 None:intergenic 40.0%
! TTTGGTTTCAGGATACCAGA+GGG + chr3.4:48522127-48522146 MS.gene027111:CDS 40.0%
!! CATTCCTGATGATGGTGATA+TGG + chr3.4:48522614-48522633 MS.gene027111:CDS 40.0%
!! TGCATGCATTGATTCATCAG+TGG + chr3.4:48521570-48521589 MS.gene027111:intron 40.0%
!!! TTTTTTTCCAGCAGTTCCAC+CGG + chr3.4:48522536-48522555 MS.gene027111:intron 40.0%
AAACAGCCCGTTATCGTCAT+AGG - chr3.4:48522265-48522284 None:intergenic 45.0%
AATGGATGTGGTAGATGCTG+AGG + chr3.4:48522791-48522810 MS.gene027111:CDS 45.0%
AGACGAAGACGAAGATGAGT+CGG - chr3.4:48522406-48522425 None:intergenic 45.0%
CATGGTGAAATGCAGCAACA+TGG + chr3.4:48522866-48522885 MS.gene027111:CDS 45.0%
CCCCAATACACTTCCATATC+TGG + chr3.4:48521535-48521554 MS.gene027111:intron 45.0%
CGATGTTGAATACCCTCCTT+TGG - chr3.4:48521421-48521440 None:intergenic 45.0%
GAGGAACCTGCTTTCGATAT+TGG + chr3.4:48522738-48522757 MS.gene027111:CDS 45.0%
GCTTCCATTCACTCTTGACA+TGG + chr3.4:48522290-48522309 MS.gene027111:CDS 45.0%
GGAACTATGACCATAGCTCA+AGG + chr3.4:48522337-48522356 MS.gene027111:CDS 45.0%
TCGTCTACGTCATCTGATGA+TGG + chr3.4:48522432-48522451 MS.gene027111:intron 45.0%
TGACATGCAACCTTGAGCTA+TGG - chr3.4:48522350-48522369 None:intergenic 45.0%
TGTTACGGAGAGATCAATGG+CGG + chr3.4:48522165-48522184 MS.gene027111:CDS 45.0%
! AAATTTGCTCGGCGAGCATT+TGG + chr3.4:48523019-48523038 MS.gene027111:CDS 45.0%
! AATTTGCTCGGCGAGCATTT+GGG + chr3.4:48523020-48523039 MS.gene027111:CDS 45.0%
! GGATTTTGTTCTTCCACCAC+TGG - chr3.4:48522660-48522679 None:intergenic 45.0%
! GTTTGGTTTCAGGATACCAG+AGG + chr3.4:48522126-48522145 MS.gene027111:CDS 45.0%
! TATCGATTGGATACTCACGG+TGG - chr3.4:48523084-48523103 None:intergenic 45.0%
! TTTAACGCTTGCTGCGTACA+TGG + chr3.4:48522848-48522867 MS.gene027111:CDS 45.0%
!! AAGGAGGGTATTCAACATCG+CGG + chr3.4:48521421-48521440 MS.gene027111:CDS 45.0%
!! GTTGCTGTTGGTGGTGATAA+TGG + chr3.4:48522495-48522514 MS.gene027111:intron 45.0%
!!! TTTTTTCCAGCAGTTCCACC+GGG + chr3.4:48522537-48522556 MS.gene027111:intron 45.0%
!!! ATGATTTTTTTTTTAAATAA+GGG - chr3.4:48521809-48521828 None:intergenic 5.0%
!!! TATGATTTTTTTTTTAAATA+AGG - chr3.4:48521810-48521829 None:intergenic 5.0%
ACTCACGGTGGAACTCATCT+CGG - chr3.4:48523072-48523091 None:intergenic 50.0%
CATAGCCGAGATTCAGAACG+TGG + chr3.4:48522695-48522714 MS.gene027111:CDS 50.0%
CCATCGGTATATTTCAGGGG+CGG + chr3.4:48523133-48523152 MS.gene027111:CDS 50.0%
CGGTCAACCCAAACCAAGTA+TGG + chr3.4:48521441-48521460 MS.gene027111:intron 50.0%
CTCATCTCGGATCGTGATGA+AGG - chr3.4:48523059-48523078 None:intergenic 50.0%
GATACCAGAGGGTCTAGGTT+TGG + chr3.4:48522138-48522157 MS.gene027111:CDS 50.0%
GGTATATTTCAGGGGCGGTT+GGG + chr3.4:48523138-48523157 MS.gene027111:CDS 50.0%
GTAGATGCTGAGGATGCACA+TGG + chr3.4:48522801-48522820 MS.gene027111:CDS 50.0%
GTATATTTCAGGGGCGGTTG+GGG + chr3.4:48523139-48523158 MS.gene027111:CDS 50.0%
GTTCTTCCACCACTGGTACT+GGG - chr3.4:48522653-48522672 None:intergenic 50.0%
TACTCCAAACCTAGACCCTC+TGG - chr3.4:48522145-48522164 None:intergenic 50.0%
TCACCATCATCAGGAATGGG+AGG - chr3.4:48522612-48522631 None:intergenic 50.0%
TCTCCTCCCATTCCTGATGA+TGG + chr3.4:48522606-48522625 MS.gene027111:CDS 50.0%
TCTCGGATCGTGATGAAGGT+TGG - chr3.4:48523055-48523074 None:intergenic 50.0%
TGTTCTTCCACCACTGGTAC+TGG - chr3.4:48522654-48522673 None:intergenic 50.0%
TTCAGGATACCAGAGGGTCT+AGG + chr3.4:48522133-48522152 MS.gene027111:CDS 50.0%
! CACGTTCTGAATCTCGGCTA+TGG - chr3.4:48522697-48522716 None:intergenic 50.0%
! TGATAATGAGGAGGCCGCTA+TGG + chr3.4:48522761-48522780 MS.gene027111:CDS 50.0%
CCGCCCCTGAAATATACCGA+TGG - chr3.4:48523136-48523155 None:intergenic 55.0%
CGGTATATTTCAGGGGCGGT+TGG + chr3.4:48523137-48523156 MS.gene027111:CDS 55.0%
CTGAATCTCGGCTATGGCAG+CGG - chr3.4:48522691-48522710 None:intergenic 55.0%
GCTGCTGCTATTGTGGGTTC+TGG + chr3.4:48522471-48522490 MS.gene027111:intron 55.0%
GGCCGCTATGGATGTCGAAA+TGG + chr3.4:48522773-48522792 MS.gene027111:CDS 55.0%
GGCGGTGGTTGCTTCTATTC+AGG + chr3.4:48522183-48522202 MS.gene027111:CDS 55.0%
GTTCCACCGGGTTTCGAAGT+AGG + chr3.4:48522549-48522568 MS.gene027111:CDS 55.0%
TACGGAGAGATCAATGGCGG+TGG + chr3.4:48522168-48522187 MS.gene027111:CDS 55.0%
TGCACATGGCAATCCCCAGT+TGG + chr3.4:48522815-48522834 MS.gene027111:CDS 55.0%
TGTTCCTGACCCAGTACCAG+TGG + chr3.4:48522641-48522660 MS.gene027111:CDS 55.0%
! GCTCGCCGAGCAAATTTTGC+TGG - chr3.4:48523016-48523035 None:intergenic 55.0%
!! GGTTCTGGTGTTGCTGTTGG+TGG + chr3.4:48522486-48522505 MS.gene027111:intron 55.0%
!! GTGGGTTCTGGTGTTGCTGT+TGG + chr3.4:48522483-48522502 MS.gene027111:intron 55.0%
AACTGTCCTGCACGTGAGGC+AGG + chr3.4:48523231-48523250 MS.gene027111:CDS 60.0%
ACCCAACTGTCCTGCACGTG+AGG + chr3.4:48523227-48523246 MS.gene027111:CDS 60.0%
AGCTTACGGCTCGTGTCCCA+TGG + chr3.4:48522064-48522083 MS.gene027111:intron 60.0%
CCACCGGGTTTCGAAGTAGG+AGG + chr3.4:48522552-48522571 MS.gene027111:CDS 60.0%
CCTCCTACTTCGAAACCCGG+TGG - chr3.4:48522555-48522574 None:intergenic 60.0%
GCACGTGAGGCAGGTCGTTA+TGG + chr3.4:48523240-48523259 MS.gene027111:CDS 60.0%
GCCTCACGTGCAGGACAGTT+GGG - chr3.4:48523231-48523250 None:intergenic 60.0%
GCTATGGCAGCGGCAGTTTG+TGG - chr3.4:48522681-48522700 None:intergenic 60.0%
GGGCCTCCTACTTCGAAACC+CGG - chr3.4:48522558-48522577 None:intergenic 60.0%
TAACGACCTGCCTCACGTGC+AGG - chr3.4:48523240-48523259 None:intergenic 60.0%
TCCTGACCCAGTACCAGTGG+TGG + chr3.4:48522644-48522663 MS.gene027111:CDS 60.0%
TCGAAACCCGGTGGAACTGC+TGG - chr3.4:48522546-48522565 None:intergenic 60.0%
TGCCTCACGTGCAGGACAGT+TGG - chr3.4:48523232-48523251 None:intergenic 60.0%
! TCCACCACTGGTACTGGGTC+AGG - chr3.4:48522648-48522667 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 48521410 48523267 48521410 ID=MS.gene027111
chr3.4 mRNA 48521410 48523267 48521410 ID=MS.gene027111.t1;Parent=MS.gene027111
chr3.4 exon 48521410 48521457 48521410 ID=MS.gene027111.t1.exon1;Parent=MS.gene027111.t1
chr3.4 CDS 48521410 48521457 48521410 ID=cds.MS.gene027111.t1;Parent=MS.gene027111.t1
chr3.4 exon 48522066 48522381 48522066 ID=MS.gene027111.t1.exon2;Parent=MS.gene027111.t1
chr3.4 CDS 48522066 48522381 48522066 ID=cds.MS.gene027111.t1;Parent=MS.gene027111.t1
chr3.4 exon 48522547 48522887 48522547 ID=MS.gene027111.t1.exon3;Parent=MS.gene027111.t1
chr3.4 CDS 48522547 48522887 48522547 ID=cds.MS.gene027111.t1;Parent=MS.gene027111.t1
chr3.4 exon 48523007 48523267 48523007 ID=MS.gene027111.t1.exon4;Parent=MS.gene027111.t1
chr3.4 CDS 48523007 48523267 48523007 ID=cds.MS.gene027111.t1;Parent=MS.gene027111.t1
Gene Sequence

>MS.gene027111

ATGGTTTTCCAAAGGAGGGTATTCAACATCGCGGTCAACCCAAACCAAGTATGGTGCTCTAATCTTCAACAACCAATGATTCAAAATGCGATGCTAAAAAGTTCTTCAGACTGATTGTTTTTTTTCCCCAATACACTTCCATATCTGGTGGATGTTGCATTGCATGCATTGATTCATCAGTGGCGCAGTAATATTGATGACTCTGATTATGTTATTTTTTATTTCCATTTTTTTTTCTTCTTAAATACAGATTGTTCAATATTGTAGTTGTAGATTGCATGCATTGATTCATCAGTGGCTCAGTATATTGATGACTCTGATTATGTTATTTATTTTTTCTTTTTTTTTTTATTTTGTTTTTTAAATACAGATTGTTCAATGTTGTAGTTGTAGATTCCCTTATTTAAAAAAAAAATCATAATTTTGTTCCTCATACACTTCCATTCTTTGTATTTTGTTGTGTTGTGTCAAATAAATGGTTATTACCAGTTTTTCTCTTTTTTGAAGGATTATTACTAGTTTTGTATTGCTCTTCTTTTTTGTATAATTTCTTCGAAAAACACAACATTAAATAATAATACCCTCGCCTTTGCTCATAGATTTTCAATACTTTTGGTTGTAAACATATTGTTGTTGTTGTTGTTTGTATTCTGTAGCTTACGGCTCGTGTCCCATGGTATTTTTACGAACGTTTCAAAGGTCAAATAGAGCATAGAGTTTGGTTTCAGGATACCAGAGGGTCTAGGTTTGGAGTATGTTACGGAGAGATCAATGGCGGTGGTTGCTTCTATTCAGGTCTTCAGAACCTGCTTGATCATTATGAGCAACCATTAGGTCTAAACCTACGATTGACCTATGACGATAACGGGCTGTTTCATATGCTTCCATTCACTCTTGACATGGATGAGGTAACTAATCGTCAATTGAGGAACTATGACCATAGCTCAAGGTTGCATGTCAATGAGTTCCTGCGTACATTTAGATACTTTGATTCCGACTCATCTTCGTCTTCGTCTTCGTCTTCGTCTACGTCATCTGATGATGGCCAATTTCAAATTAATGCTGCTGCTATTGTGGGTTCTGGTGTTGCTGTTGGTGGTGATAATGGTGCTGTTGCTGCTGCTGATTTTTTTCCAGCAGTTCCACCGGGTTTCGAAGTAGGAGGCCCGTCAAATCCAATTAATGATGCGCACAACTCTCCTCCCATTCCTGATGATGGTGATATGGTAGTTGTTCCTGACCCAGTACCAGTGGTGGAAGAACAAAATCCACAAACTGCCGCTGCCATAGCCGAGATTCAGAACGTGGCTAATTTCTGGGATGATGATGAGGAACCTGCTTTCGATATTGGTGATAATGAGGAGGCCGCTATGGATGTCGAAATGGATGTGGTAGATGCTGAGGATGCACATGGCAATCCCCAGTTGGAGACACATGTTTTAACGCTTGCTGCGTACATGGTGAAATGCAGCAACATGGTGAGTTACTTTGAAAAAATAAAAAAAAAACATATCCACGCTTTATACAGTTATCTATTGTGGTTTGGCAAGTGTGTGACTGTTGACATGAAATAATTATTGTTATCTGTCAACTGCAGCTTGTACCAGCAAAATTTGCTCGGCGAGCATTTGGGAATACAAGACCAACCTTCATCACGATCCGAGATGAGTTCCACCGTGAGTATCCAATCGATATTAAGTGGTATGAAAAACCGCATAAATCTCCATCGGTATATTTCAGGGGCGGTTGGGGTGAATTCGTTAGAGAGAACAAATTTGTGGAAGGCACAAGGGTGTTGCTCACTTTAAACAAGACTAACCCAACTGTCCTGCACGTGAGGCAGGTCGTTATGGTGTAA
Protein sequence

>MS.gene027111.t1

MVFQRRVFNIAVNPNQLTARVPWYFYERFKGQIEHRVWFQDTRGSRFGVCYGEINGGGCFYSGLQNLLDHYEQPLGLNLRLTYDDNGLFHMLPFTLDMDEVTNRQLRNYDHSSRLHVNEFLPVPPGFEVGGPSNPINDAHNSPPIPDDGDMVVVPDPVPVVEEQNPQTAAAIAEIQNVANFWDDDEEPAFDIGDNEEAAMDVEMDVVDAEDAHGNPQLETHVLTLAAYMVKCSNMLVPAKFARRAFGNTRPTFITIRDEFHREYPIDIKWYEKPHKSPSVYFRGGWGEFVRENKFVEGTRVLLTLNKTNPTVLHVRQVVMV