AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027327

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027327.t1 MTR_7g053410 96.552 464 6 2 1 460 19 476 0.0 910
MS.gene027327.t1 MTR_7g053410 96.552 464 6 2 1 460 19 476 0.0 909
MS.gene027327.t1 MTR_8g012290 62.791 473 116 14 12 460 15 451 2.25e-175 501
MS.gene027327.t1 MTR_8g012290 62.791 473 116 14 12 460 15 451 3.51e-175 500
MS.gene027327.t1 MTR_1g017350 36.979 384 169 11 79 426 74 420 2.29e-54 187
MS.gene027327.t1 MTR_1g017350 36.788 386 171 11 79 428 74 422 2.60e-54 187
MS.gene027327.t1 MTR_1g017350 37.008 381 170 11 79 426 74 417 4.08e-53 184
MS.gene027327.t1 MTR_1g017350 36.815 383 172 11 79 428 74 419 6.12e-53 184
MS.gene027327.t1 MTR_8g065740 38.310 355 163 13 116 440 125 453 1.58e-52 184
MS.gene027327.t1 MTR_4g070320 58.235 170 56 4 246 408 85 246 1.22e-51 178
MS.gene027327.t1 MTR_3g116770 65.468 139 44 2 254 388 140 278 7.65e-51 177
MS.gene027327.t1 MTR_3g116770 65.468 139 44 2 254 388 140 278 7.83e-51 177
MS.gene027327.t1 MTR_8g062240 51.724 203 82 4 217 412 76 269 9.31e-50 171
MS.gene027327.t1 MTR_5g037250 57.333 150 47 2 240 389 68 200 2.73e-44 155
MS.gene027327.t1 MTR_8g099880 69.643 112 31 2 293 401 137 248 1.27e-43 156
MS.gene027327.t1 MTR_7g092510 59.091 132 43 3 298 420 170 299 2.18e-42 153
MS.gene027327.t1 MTR_3g498825 88.750 80 9 0 293 372 139 218 2.41e-41 150
MS.gene027327.t1 MTR_1g059270 68.132 91 29 0 298 388 157 247 3.03e-39 142
MS.gene027327.t1 MTR_1g059270 69.663 89 27 0 298 386 157 245 9.43e-39 143
MS.gene027327.t1 MTR_6g084120 53.636 110 42 1 272 381 126 226 7.70e-29 114
MS.gene027327.t1 MTR_4g131160 55.660 106 38 4 301 400 196 298 1.71e-22 99.4
MS.gene027327.t1 MTR_4g131160 72.727 66 17 1 301 366 199 263 2.09e-22 99.4
MS.gene027327.t1 MTR_1g080890 64.000 75 26 1 292 366 184 257 4.72e-22 97.4
MS.gene027327.t1 MTR_2g098310 35.115 131 76 2 249 371 273 402 1.71e-15 78.6
MS.gene027327.t1 MTR_1g070870 60.000 75 29 1 292 366 111 184 2.48e-15 76.6
MS.gene027327.t1 MTR_4g065870 58.462 65 27 0 301 365 355 419 2.75e-15 78.2
MS.gene027327.t1 MTR_1g070870 60.000 75 29 1 292 366 114 187 2.78e-15 76.6
MS.gene027327.t1 MTR_4g033295 55.385 65 29 0 301 365 118 182 3.86e-15 74.3
MS.gene027327.t1 MTR_4g033295 53.731 67 30 1 301 366 118 184 4.94e-15 73.6
MS.gene027327.t1 MTR_2g098310 54.545 66 30 0 299 364 330 395 6.47e-15 76.6
MS.gene027327.t1 MTR_1g069155 52.174 69 32 1 303 371 319 386 1.01e-14 76.6
MS.gene027327.t1 MTR_4g065870 58.065 62 26 0 301 362 355 416 1.25e-14 75.9
MS.gene027327.t1 MTR_7g092540 48.649 74 38 0 301 374 243 316 1.33e-14 75.1
MS.gene027327.t1 MTR_4g107320 51.316 76 35 2 319 392 60 135 1.50e-14 70.9
MS.gene027327.t1 MTR_1g052470 52.174 69 33 0 301 369 298 366 2.23e-14 75.1
MS.gene027327.t1 MTR_1g084980 51.562 64 30 1 303 366 464 526 3.50e-14 75.5
MS.gene027327.t1 MTR_7g092540 51.562 64 31 0 301 364 243 306 1.03e-13 72.0
MS.gene027327.t1 MTR_7g099540 46.835 79 41 1 293 371 244 321 3.35e-13 71.6
MS.gene027327.t1 MTR_3g028520 50.769 65 32 0 301 365 322 386 5.33e-13 70.9
MS.gene027327.t1 MTR_3g027650 50.769 65 32 0 301 365 322 386 5.33e-13 70.9
MS.gene027327.t1 MTR_1g019240 39.560 91 47 2 277 367 68 150 1.70e-12 67.8
MS.gene027327.t1 MTR_8g036235 47.692 65 34 0 301 365 316 380 2.10e-12 68.9
MS.gene027327.t1 MTR_3g027650 51.613 62 30 0 301 362 322 383 2.25e-12 68.9
MS.gene027327.t1 MTR_1g018090 41.000 100 39 2 266 365 258 337 3.06e-12 68.2
MS.gene027327.t1 MTR_5g005110 51.562 64 30 1 302 365 225 287 7.69e-12 66.6
MS.gene027327.t1 MTR_3g103030 53.125 64 29 1 302 365 230 292 1.51e-11 65.9
MS.gene027327.t1 MTR_7g099540 45.946 74 39 1 293 366 244 316 1.68e-11 66.6
MS.gene027327.t1 MTR_3g449770 50.000 64 31 1 303 366 311 373 1.85e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027327.t1 AT3G07340 45.824 467 169 12 1 460 26 415 7.38e-112 337
MS.gene027327.t1 AT3G07340 45.824 467 169 12 1 460 26 415 9.14e-112 337
MS.gene027327.t1 AT5G48560 47.433 487 177 20 1 460 32 466 3.23e-98 303
MS.gene027327.t1 AT5G48560 47.433 487 177 20 1 460 32 466 1.11e-97 302
MS.gene027327.t1 AT3G23690 57.071 198 73 4 216 409 113 302 1.34e-59 200
MS.gene027327.t1 AT1G68920 43.284 335 142 13 96 400 97 413 1.42e-58 200
MS.gene027327.t1 AT1G68920 41.493 335 149 10 96 400 97 414 2.27e-58 199
MS.gene027327.t1 AT1G68920 41.493 335 149 10 96 400 97 414 2.27e-58 199
MS.gene027327.t1 AT1G68920 41.493 335 149 10 96 400 97 414 2.27e-58 199
MS.gene027327.t1 AT1G26260 42.088 297 119 10 111 400 68 318 2.72e-53 184
MS.gene027327.t1 AT1G26260 42.088 297 119 10 111 400 68 318 2.72e-53 184
MS.gene027327.t1 AT1G26260 42.088 297 119 10 111 400 68 318 2.72e-53 184
MS.gene027327.t1 AT1G26260 42.088 297 119 10 111 400 68 318 2.72e-53 184
MS.gene027327.t1 AT1G26260 42.756 283 109 10 125 400 60 296 2.68e-50 175
MS.gene027327.t1 AT1G59640 53.631 179 59 3 242 420 79 233 5.22e-50 171
MS.gene027327.t1 AT1G10120 68.908 119 36 1 275 392 179 297 2.75e-49 172
MS.gene027327.t1 AT1G10120 68.908 119 36 1 275 392 179 297 2.75e-49 172
MS.gene027327.t1 AT1G26260 59.615 156 51 4 246 400 123 267 6.23e-49 171
MS.gene027327.t1 AT1G59640 60.274 146 56 1 242 387 79 222 8.19e-48 168
MS.gene027327.t1 AT4G34530 56.098 164 50 4 242 387 99 258 4.88e-47 166
MS.gene027327.t1 AT2G18300 55.152 165 61 4 251 402 129 293 2.96e-46 163
MS.gene027327.t1 AT2G18300 55.952 168 57 6 251 402 129 295 6.38e-46 162
MS.gene027327.t1 AT2G18300 55.952 168 57 6 251 402 129 295 1.04e-45 163
MS.gene027327.t1 AT5G50915 50.476 210 79 10 216 411 62 260 1.12e-44 157
MS.gene027327.t1 AT5G50915 50.476 210 79 10 216 411 62 260 1.12e-44 157
MS.gene027327.t1 AT5G50915 49.533 214 83 10 216 415 62 264 2.67e-44 157
MS.gene027327.t1 AT5G50915 49.533 214 83 10 216 415 62 264 2.67e-44 157
MS.gene027327.t1 AT4G36540 58.571 140 54 2 249 388 93 228 2.52e-43 155
MS.gene027327.t1 AT4G36540 59.155 142 53 3 249 390 93 229 4.01e-43 154
MS.gene027327.t1 AT5G62610 76.087 92 17 1 282 373 139 225 1.42e-42 152
MS.gene027327.t1 AT5G62610 76.087 92 17 1 282 373 139 225 1.42e-42 152
MS.gene027327.t1 AT2G42300 70.000 90 27 0 298 387 182 271 9.78e-41 147
MS.gene027327.t1 AT3G57800 57.252 131 41 3 298 417 201 327 1.17e-40 149
MS.gene027327.t1 AT2G42300 61.667 120 36 3 298 417 182 291 4.05e-40 147
MS.gene027327.t1 AT1G25330 59.292 113 37 1 280 392 92 195 8.21e-37 135
MS.gene027327.t1 AT1G18400 72.289 83 23 0 299 381 143 225 7.24e-35 130
MS.gene027327.t1 AT1G18400 72.289 83 23 0 299 381 143 225 1.26e-34 130
MS.gene027327.t1 AT1G73830 73.171 82 22 0 300 381 146 227 1.72e-34 130
MS.gene027327.t1 AT1G73830 73.171 82 22 0 300 381 146 227 1.88e-34 130
MS.gene027327.t1 AT3G57800 42.135 178 41 4 298 417 201 374 5.78e-32 127
MS.gene027327.t1 AT2G24260 66.667 72 23 1 295 366 29 99 6.80e-24 100
MS.gene027327.t1 AT2G24260 66.667 72 23 1 295 366 132 202 6.98e-23 99.8
MS.gene027327.t1 AT4G30980 51.961 102 38 2 265 366 104 194 7.36e-23 99.0
MS.gene027327.t1 AT5G58010 54.545 99 44 1 268 366 66 163 9.00e-23 98.6
MS.gene027327.t1 AT4G30980 52.941 102 37 3 265 366 152 242 1.46e-22 99.0
MS.gene027327.t1 AT2G42300 83.721 43 7 0 298 340 182 224 1.04e-18 85.5
MS.gene027327.t1 AT2G42300 83.721 43 7 0 298 340 182 224 1.04e-18 85.5
MS.gene027327.t1 AT2G42300 83.721 43 7 0 298 340 182 224 1.26e-18 85.1
MS.gene027327.t1 AT4G28790 38.095 126 60 3 241 366 228 335 5.79e-15 77.0
MS.gene027327.t1 AT2G20180 53.968 63 28 1 304 366 210 271 1.68e-14 75.5
MS.gene027327.t1 AT2G20180 53.968 63 28 1 304 366 210 271 1.68e-14 75.5
MS.gene027327.t1 AT2G20180 53.968 63 28 1 304 366 210 271 1.68e-14 75.5
MS.gene027327.t1 AT4G09180 52.239 67 32 0 299 365 182 248 1.69e-14 73.9
MS.gene027327.t1 AT2G20180 53.968 63 28 1 304 366 281 342 2.10e-14 75.5
MS.gene027327.t1 AT2G20180 53.968 63 28 1 304 366 281 342 2.10e-14 75.5
MS.gene027327.t1 AT2G20180 53.968 63 28 1 304 366 281 342 2.10e-14 75.5
MS.gene027327.t1 AT2G20180 53.968 63 28 1 304 366 281 342 2.10e-14 75.5
MS.gene027327.t1 AT4G02590 57.500 80 29 2 291 366 69 147 2.26e-14 73.2
MS.gene027327.t1 AT4G02590 57.500 80 29 2 291 366 132 210 3.18e-14 73.9
MS.gene027327.t1 AT4G02590 57.500 80 29 2 291 366 132 210 3.18e-14 73.9
MS.gene027327.t1 AT2G42280 53.125 64 30 0 301 364 279 342 3.26e-14 74.3
MS.gene027327.t1 AT1G51140 56.250 64 28 0 301 364 304 367 3.78e-14 74.3
MS.gene027327.t1 AT1G09530 51.562 64 30 1 303 366 339 401 5.84e-14 74.3
MS.gene027327.t1 AT1G09530 51.562 64 30 1 303 366 339 401 5.84e-14 74.3
MS.gene027327.t1 AT1G09530 51.562 64 30 1 303 366 339 401 5.84e-14 74.3
MS.gene027327.t1 AT1G09530 51.562 64 30 1 303 366 339 401 5.84e-14 74.3
MS.gene027327.t1 AT1G09530 51.562 64 30 1 303 366 339 401 5.84e-14 74.3
MS.gene027327.t1 AT1G09530 51.562 64 30 1 303 366 339 401 5.84e-14 74.3
MS.gene027327.t1 AT2G42280 54.545 66 28 1 301 364 279 344 5.93e-14 73.6
MS.gene027327.t1 AT4G28790 35.938 128 64 3 241 368 228 337 8.93e-14 72.8
MS.gene027327.t1 AT1G35460 52.308 65 31 0 301 365 181 245 1.16e-13 71.2
MS.gene027327.t1 AT1G03040 63.636 66 23 1 301 366 139 203 1.18e-13 72.0
MS.gene027327.t1 AT1G03040 63.636 66 23 1 301 366 144 208 1.31e-13 72.0
MS.gene027327.t1 AT4G28815 56.897 58 24 1 304 361 71 127 1.51e-13 70.5
MS.gene027327.t1 AT1G03040 63.636 66 23 1 301 366 144 208 2.70e-13 71.2
MS.gene027327.t1 AT4G28800 35.938 128 65 3 238 365 202 312 3.17e-13 72.0
MS.gene027327.t1 AT2G20180 53.333 60 27 1 304 363 281 339 5.63e-13 70.5
MS.gene027327.t1 AT4G28815 56.897 58 24 1 304 361 147 203 5.86e-13 70.1
MS.gene027327.t1 AT4G33880 43.182 88 49 1 278 365 243 329 9.59e-13 69.7
MS.gene027327.t1 AT4G28800 36.000 125 63 3 238 362 214 321 1.08e-12 70.1
MS.gene027327.t1 AT2G14760 42.857 91 51 1 275 365 211 300 1.25e-12 69.3
MS.gene027327.t1 AT2G46970 34.211 114 57 3 258 366 187 287 2.52e-12 68.9
MS.gene027327.t1 AT2G46970 34.211 114 57 3 258 366 156 256 2.79e-12 68.6
MS.gene027327.t1 AT2G46970 34.211 114 57 3 258 366 165 265 2.89e-12 68.6
MS.gene027327.t1 AT2G43140 48.611 72 35 1 295 364 126 197 1.04e-11 64.7
MS.gene027327.t1 AT1G27740 43.617 94 44 2 272 365 146 230 1.16e-11 65.5
MS.gene027327.t1 AT2G43140 48.611 72 35 1 295 364 223 294 1.38e-11 65.9
MS.gene027327.t1 AT4G00050 50.769 65 31 1 301 365 105 168 3.16e-11 64.7
MS.gene027327.t1 AT4G00050 50.769 65 31 1 301 365 207 270 5.75e-11 64.7
MS.gene027327.t1 AT4G00050 50.769 65 31 1 301 365 207 270 6.59e-11 64.3
MS.gene027327.t1 AT4G36930 50.820 61 29 1 307 367 197 256 7.52e-11 63.9

Find 83 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATTCCTTCTAGGTTAC+TGG 0.213478 7.1:+48792279 MS.gene027327:intron
AGAGAATTGATTGGTAAATT+AGG 0.220575 7.1:+48790997 MS.gene027327:CDS
TCAAAGATCTACTCCATTAT+TGG 0.223521 7.1:+48791272 MS.gene027327:CDS
TCCTCAGCACCATCCTATTA+TGG 0.234641 7.1:+48792839 MS.gene027327:CDS
GCAGTTCCTATCTATGAAAT+TGG 0.256565 7.1:+48792602 MS.gene027327:intron
TCTACTCCATTATTGGAAAA+TGG 0.261666 7.1:+48791279 MS.gene027327:CDS
GTTTCAATCAAATAATTCTT+TGG 0.281320 7.1:+48792793 MS.gene027327:CDS
GAGGAATCTAATGGGAATAT+TGG 0.295383 7.1:-48792821 None:intergenic
ATAAGTGCATGCCAAGGTTT+TGG 0.306516 7.1:-48792955 None:intergenic
ACTAGAAGTTGAAGTTTCTT+TGG 0.309086 7.1:-48791502 None:intergenic
ATTCACAATAATATTTCTAA+TGG 0.321191 7.1:+48792887 MS.gene027327:CDS
TTCTGCAACAGTTGAATTCA+TGG 0.325717 7.1:-48791154 None:intergenic
GAATCAACAATCTCTGAAAA+TGG 0.326758 7.1:+48791420 MS.gene027327:CDS
ATTGTTGAGATGTTCAATTT+TGG 0.329946 7.1:-48791129 None:intergenic
GAAATATTATTGTGAATTGC+TGG 0.332246 7.1:-48792881 None:intergenic
TTACTCTTTCTGCAAGACTA+TGG 0.332464 7.1:-48791858 None:intergenic
CTTCTCCTTATGTGAATTCA+AGG 0.338861 7.1:+48791451 MS.gene027327:CDS
TCTTGTCCCTGGTTGCAATA+AGG 0.355254 7.1:+48792181 MS.gene027327:CDS
TGGCATAAACCACCATCTAA+TGG 0.361230 7.1:-48792935 None:intergenic
GCTGCAAAATTCTCTTGCTT+TGG 0.361360 7.1:+48791207 MS.gene027327:CDS
GAATTGTTCTACTCAATTGC+AGG 0.367833 7.1:+48791368 MS.gene027327:CDS
GGCATAAACCACCATCTAAT+GGG 0.369801 7.1:-48792934 None:intergenic
CAGTGGCCAATTTCATAGAT+AGG 0.382519 7.1:-48792608 None:intergenic
CCTCAGCACCATCCTATTAT+GGG 0.394260 7.1:+48792840 MS.gene027327:CDS
AGGAATCTAATGGGAATATT+GGG 0.404291 7.1:-48792820 None:intergenic
GATCCAAGTTCTTTCAATGA+TGG 0.405954 7.1:-48791327 None:intergenic
AATTCTCTTGCTTTGGAAGT+AGG 0.408436 7.1:+48791214 MS.gene027327:CDS
CTGGTGTTGTTGCCCATAAT+AGG 0.412733 7.1:-48792852 None:intergenic
GATGGTGCTGAGGAATCTAA+TGG 0.422338 7.1:-48792830 None:intergenic
GTTGTTGACTTATCCCATAT+TGG 0.422874 7.1:-48790877 None:intergenic
GCAGAATTCTCAACTGATCC+TGG 0.423123 7.1:+48791171 MS.gene027327:CDS
TGATTCAGCACTTAGTTCAA+TGG 0.424451 7.1:+48790921 MS.gene027327:CDS
AAGCAGAGGAAGAGTCAAAA+GGG 0.435371 7.1:+48791719 MS.gene027327:CDS
CTAAGTGGATTTAATGAAGC+TGG 0.440757 7.1:+48792983 MS.gene027327:CDS
TTTGTCATCAGAGAATTGAT+TGG 0.446145 7.1:+48790988 MS.gene027327:CDS
ATTGGTAAATTAGGAAACAT+TGG 0.447086 7.1:+48791006 MS.gene027327:CDS
AACTCAGTGGACCCATTAGA+TGG 0.450062 7.1:+48792923 MS.gene027327:CDS
ATGTAATCCTTTGGTGCCTC+AGG 0.463142 7.1:-48791799 None:intergenic
AGTCCATCATTGAAAGAACT+TGG 0.466629 7.1:+48791324 MS.gene027327:CDS
AAAATTGTCATTTGACATGT+TGG 0.469622 7.1:-48790968 None:intergenic
CTGACATGAATGTAATCCTT+TGG 0.473631 7.1:-48791808 None:intergenic
TAATGGGTCCACTGAGTTGT+GGG 0.475871 7.1:-48792918 None:intergenic
AAATATTATTGTGAATTGCT+GGG 0.490799 7.1:-48792880 None:intergenic
CTAATGGGTCCACTGAGTTG+TGG 0.497924 7.1:-48792919 None:intergenic
CTTGAATTCACATAAGGAGA+AGG 0.513147 7.1:-48791450 None:intergenic
GCAGCTCTTTCTGCAAAACC+AGG 0.515215 7.1:-48791189 None:intergenic
TTTGGAAGTAGGAGTTTCAA+TGG 0.516264 7.1:+48791225 MS.gene027327:CDS
AAAGCAGAGGAAGAGTCAAA+AGG 0.521509 7.1:+48791718 MS.gene027327:CDS
AAAAGAAAAGTTTCTTCCAA+AGG 0.532200 7.1:+48791474 MS.gene027327:CDS
TGATGAGATCTCACAAACAT+TGG 0.547828 7.1:+48791038 MS.gene027327:CDS
AACCATCTTCAATGCCTACA+TGG 0.550038 7.1:+48790809 None:intergenic
CTTCTACAAGATCTTGTCCC+TGG 0.550938 7.1:+48792170 MS.gene027327:CDS
ATTTAATGAAGCTGGCTCAC+AGG 0.551026 7.1:+48792991 MS.gene027327:CDS
ATGGTGCTGAGGAATCTAAT+GGG 0.559576 7.1:-48792829 None:intergenic
GGAGGGAGAAAATCAGTGAA+AGG 0.560766 7.1:+48792141 MS.gene027327:CDS
AAGCGCATGAAAACAAATGA+AGG 0.563724 7.1:+48791670 MS.gene027327:CDS
AAAACCTCCTGAGGCACCAA+AGG 0.566429 7.1:+48791792 MS.gene027327:CDS
GATGAAAGTGATTGCCATGT+AGG 0.566700 7.1:-48790823 None:intergenic
TAATCCTTTGGTGCCTCAGG+AGG 0.569593 7.1:-48791796 None:intergenic
ATCATTGCAGCGTCAAGTTG+AGG 0.580066 7.1:+48792341 MS.gene027327:CDS
ATGCACTTATCTTCACTAAG+TGG 0.581033 7.1:+48792968 MS.gene027327:CDS
GGTGGTTTATGCCAAAACCT+TGG 0.587041 7.1:+48792944 MS.gene027327:CDS
AATTGGCCACTGTTAACACC+AGG 0.602763 7.1:+48792619 MS.gene027327:CDS
AACTTCTAGTAACCCTGCAA+TGG 0.607773 7.1:+48791515 MS.gene027327:CDS
CTCAATACTAAAATCCACCC+TGG 0.620586 7.1:-48792637 None:intergenic
ATGTTGGAATTTGAAGCTGC+AGG 0.621377 7.1:-48790952 None:intergenic
GGTACAAATAGTAATAATGG+AGG 0.627017 7.1:+48791742 MS.gene027327:CDS
AAAATTGAACATCTCAACAA+TGG 0.629044 7.1:+48791131 MS.gene027327:CDS
TACCAACCTTATTGCAACCA+GGG 0.629054 7.1:-48792187 None:intergenic
TGTTGTTGCCCATAATAGGA+TGG 0.639120 7.1:-48792848 None:intergenic
TTACCAACCTTATTGCAACC+AGG 0.639673 7.1:-48792188 None:intergenic
GTGAAGATAAGTGCATGCCA+AGG 0.643919 7.1:-48792961 None:intergenic
AAATGGTAAAGTAAAAGCAG+AGG 0.646618 7.1:+48791705 MS.gene027327:CDS
ATTCATGTCAGAGCAAGAAG+AGG 0.647303 7.1:+48791820 MS.gene027327:CDS
AGCAGAGGAAGAGTCAAAAG+GGG 0.656584 7.1:+48791720 MS.gene027327:CDS
GAGCAACTCAAAACCTCCTG+AGG 0.656602 7.1:+48791783 MS.gene027327:CDS
CCCATAATAGGATGGTGCTG+AGG 0.665748 7.1:-48792840 None:intergenic
AACTGTGACCCACAACTCAG+TGG 0.673850 7.1:+48792910 MS.gene027327:CDS
TCAGTGGACCCATTAGATGG+TGG 0.680214 7.1:+48792926 MS.gene027327:CDS
ATCCACCCTGGTGTTAACAG+TGG 0.694539 7.1:-48792625 None:intergenic
GCAGAGGAAGAGTCAAAAGG+GGG 0.705450 7.1:+48791721 MS.gene027327:CDS
GGCCACTGTTAACACCAGGG+TGG 0.729118 7.1:+48792623 MS.gene027327:CDS
ATTGGCCACTGTTAACACCA+GGG 0.731202 7.1:+48792620 MS.gene027327:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTATATTTAAAAT+TGG + chr7.1:48792488-48792507 MS.gene027327:intron 0.0%
!!! TTTTTATATTTAAAATTGGA+AGG + chr7.1:48792492-48792511 MS.gene027327:intron 10.0%
!! AAATGACTAATTTAATTAAG+AGG - chr7.1:48791579-48791598 None:intergenic 15.0%
!! AATGACTAATTTAATTAAGA+GGG - chr7.1:48791578-48791597 None:intergenic 15.0%
!!! AAAAAGTCATGTAATTTTTA+AGG - chr7.1:48792466-48792485 None:intergenic 15.0%
!!! ATTCACAATAATATTTCTAA+TGG + chr7.1:48792887-48792906 MS.gene027327:CDS 15.0%
!!! TGTAAACTAATTTTTGTTAT+AGG + chr7.1:48792115-48792134 MS.gene027327:intron 15.0%
!! AAATATTATTGTGAATTGCT+GGG - chr7.1:48792883-48792902 None:intergenic 20.0%
!! GGTAATACTTAGATAAATTA+AGG + chr7.1:48792206-48792225 MS.gene027327:intron 20.0%
!! GTTTCAATCAAATAATTCTT+TGG + chr7.1:48792793-48792812 MS.gene027327:CDS 20.0%
!! TATGAATTATTATTCCTTCT+AGG + chr7.1:48792272-48792291 MS.gene027327:intron 20.0%
!! TTAAGGATTCATAATTTGAT+TGG + chr7.1:48792223-48792242 MS.gene027327:intron 20.0%
!! TTCATATTCTAAATCCAAAA+GGG + chr7.1:48791597-48791616 MS.gene027327:intron 20.0%
!! TTTCATATTCTAAATCCAAA+AGG + chr7.1:48791596-48791615 MS.gene027327:intron 20.0%
!!! ACTAATTTTTGTTATAGGTT+AGG + chr7.1:48792120-48792139 MS.gene027327:intron 20.0%
!!! CATTTTTGTTTTTGCAATAT+AGG + chr7.1:48791621-48791640 MS.gene027327:intron 20.0%
! AAAAGAAAAGTTTCTTCCAA+AGG + chr7.1:48791474-48791493 MS.gene027327:CDS 25.0%
! AAAATTGAACATCTCAACAA+TGG + chr7.1:48791131-48791150 MS.gene027327:CDS 25.0%
! AGAGAATTGATTGGTAAATT+AGG + chr7.1:48790997-48791016 MS.gene027327:CDS 25.0%
! ATGATTGTTAGTGTCAATTA+TGG - chr7.1:48791907-48791926 None:intergenic 25.0%
! ATTGGTAAATTAGGAAACAT+TGG + chr7.1:48791006-48791025 MS.gene027327:CDS 25.0%
! CTCAAAAGATGTAAGTTTAA+GGG + chr7.1:48792668-48792687 MS.gene027327:intron 25.0%
! GAAATATTATTGTGAATTGC+TGG - chr7.1:48792884-48792903 None:intergenic 25.0%
! GAAGGTGAAAAAATTGAAAA+TGG + chr7.1:48791688-48791707 MS.gene027327:CDS 25.0%
! TCTCAAAAGATGTAAGTTTA+AGG + chr7.1:48792667-48792686 MS.gene027327:intron 25.0%
!! AAAAACAAAAATGACCCTTT+TGG - chr7.1:48791614-48791633 None:intergenic 25.0%
!! AAAATTGTCATTTGACATGT+TGG - chr7.1:48790971-48790990 None:intergenic 25.0%
!! AGTTTTCCATTTTCCAATAA+TGG - chr7.1:48791288-48791307 None:intergenic 25.0%
!! ATTGTTGAGATGTTCAATTT+TGG - chr7.1:48791132-48791151 None:intergenic 25.0%
!! GCATGCATACTAATTTTATT+TGG + chr7.1:48792400-48792419 MS.gene027327:intron 25.0%
!! TCGTATTATAGCATATTTTG+AGG - chr7.1:48792020-48792039 None:intergenic 25.0%
!!! AATTTTTAACTTACCATTGC+AGG - chr7.1:48791531-48791550 None:intergenic 25.0%
!!! AATTTTTGTTATAGGTTAGG+AGG + chr7.1:48792123-48792142 MS.gene027327:intron 25.0%
!!! ATTGTTTTTACACACCAATA+TGG + chr7.1:48790863-48790882 MS.gene027327:CDS 25.0%
!!! ATTTTTAACTTACCATTGCA+GGG - chr7.1:48791530-48791549 None:intergenic 25.0%
!!! ATTTTTGTTATAGGTTAGGA+GGG + chr7.1:48792124-48792143 MS.gene027327:intron 25.0%
!!! TTGTTTTTACACACCAATAT+GGG + chr7.1:48790864-48790883 MS.gene027327:CDS 25.0%
AAATGGTAAAGTAAAAGCAG+AGG + chr7.1:48791705-48791724 MS.gene027327:CDS 30.0%
ACTAGAAGTTGAAGTTTCTT+TGG - chr7.1:48791505-48791524 None:intergenic 30.0%
AGGAATCTAATGGGAATATT+GGG - chr7.1:48792823-48792842 None:intergenic 30.0%
ATGATGGCAATATACATGTT+AGG + chr7.1:48792074-48792093 MS.gene027327:intron 30.0%
CACATATTGTGGCAATATAA+AGG - chr7.1:48791957-48791976 None:intergenic 30.0%
GGTACAAATAGTAATAATGG+AGG + chr7.1:48791742-48791761 MS.gene027327:CDS 30.0%
TAACATGTATATTGCCATCA+TGG - chr7.1:48792075-48792094 None:intergenic 30.0%
TATTATTCCTTCTAGGTTAC+TGG + chr7.1:48792279-48792298 MS.gene027327:intron 30.0%
TCAAAGATCTACTCCATTAT+TGG + chr7.1:48791272-48791291 MS.gene027327:CDS 30.0%
TCTACTCCATTATTGGAAAA+TGG + chr7.1:48791279-48791298 MS.gene027327:CDS 30.0%
TGCAAAAAAAATTGTTGTCG+CGG + chr7.1:48791991-48792010 MS.gene027327:intron 30.0%
TTGGTGTGTAAAAACAATCT+TGG - chr7.1:48790861-48790880 None:intergenic 30.0%
TTTGTCATCAGAGAATTGAT+TGG + chr7.1:48790988-48791007 MS.gene027327:CDS 30.0%
! ACCACAATTTAAAACCATGA+TGG + chr7.1:48792058-48792077 MS.gene027327:intron 30.0%
! CTTTTCCTTGAATTCACATA+AGG - chr7.1:48791459-48791478 None:intergenic 30.0%
! GAATCAACAATCTCTGAAAA+TGG + chr7.1:48791420-48791439 MS.gene027327:CDS 30.0%
!! AATTTTGGAGGAGAATTCAA+AGG - chr7.1:48791117-48791136 None:intergenic 30.0%
!! ATTTTGGAGGAGAATTCAAA+GGG - chr7.1:48791116-48791135 None:intergenic 30.0%
!!! AGTTTCTTTGGTTTTTCCTT+TGG - chr7.1:48791493-48791512 None:intergenic 30.0%
AAGCGCATGAAAACAAATGA+AGG + chr7.1:48791670-48791689 MS.gene027327:CDS 35.0%
AGTCCATCATTGAAAGAACT+TGG + chr7.1:48791324-48791343 MS.gene027327:CDS 35.0%
ATGCACTTATCTTCACTAAG+TGG + chr7.1:48792968-48792987 MS.gene027327:CDS 35.0%
CTGACATGAATGTAATCCTT+TGG - chr7.1:48791811-48791830 None:intergenic 35.0%
CTTCTCCTTATGTGAATTCA+AGG + chr7.1:48791451-48791470 MS.gene027327:CDS 35.0%
CTTGAATTCACATAAGGAGA+AGG - chr7.1:48791453-48791472 None:intergenic 35.0%
GAATTGTTCTACTCAATTGC+AGG + chr7.1:48791368-48791387 MS.gene027327:CDS 35.0%
GAGGAATCTAATGGGAATAT+TGG - chr7.1:48792824-48792843 None:intergenic 35.0%
GATCCAAGTTCTTTCAATGA+TGG - chr7.1:48791330-48791349 None:intergenic 35.0%
GCAGTTCCTATCTATGAAAT+TGG + chr7.1:48792602-48792621 MS.gene027327:intron 35.0%
TGATGAGATCTCACAAACAT+TGG + chr7.1:48791038-48791057 MS.gene027327:CDS 35.0%
TGATTCAGCACTTAGTTCAA+TGG + chr7.1:48790921-48790940 MS.gene027327:CDS 35.0%
TGTCACATTGTCACATATTG+TGG - chr7.1:48791968-48791987 None:intergenic 35.0%
TTACTCTTTCTGCAAGACTA+TGG - chr7.1:48791861-48791880 None:intergenic 35.0%
TTCTGCAACAGTTGAATTCA+TGG - chr7.1:48791157-48791176 None:intergenic 35.0%
TTTGGAAGTAGGAGTTTCAA+TGG + chr7.1:48791225-48791244 MS.gene027327:CDS 35.0%
! CTAAGTGGATTTAATGAAGC+TGG + chr7.1:48792983-48793002 MS.gene027327:CDS 35.0%
! GGGGGTACAAATAGTAATAA+TGG + chr7.1:48791739-48791758 MS.gene027327:CDS 35.0%
! GTTGAGATGTTCAATTTTGG+AGG - chr7.1:48791129-48791148 None:intergenic 35.0%
!! AATTCTCTTGCTTTGGAAGT+AGG + chr7.1:48791214-48791233 MS.gene027327:CDS 35.0%
!! AGTTTAAGGGCTCTAGATAA+TGG + chr7.1:48792681-48792700 MS.gene027327:intron 35.0%
!! GTTGTTGACTTATCCCATAT+TGG - chr7.1:48790880-48790899 None:intergenic 35.0%
!! TTTTGGAGGAGAATTCAAAG+GGG - chr7.1:48791115-48791134 None:intergenic 35.0%
!!! GCCATCATGGTTTTAAATTG+TGG - chr7.1:48792062-48792081 None:intergenic 35.0%
AAAGCAGAGGAAGAGTCAAA+AGG + chr7.1:48791718-48791737 MS.gene027327:CDS 40.0%
AACTTCTAGTAACCCTGCAA+TGG + chr7.1:48791515-48791534 MS.gene027327:CDS 40.0%
AAGCAGAGGAAGAGTCAAAA+GGG + chr7.1:48791719-48791738 MS.gene027327:CDS 40.0%
ATTCATGTCAGAGCAAGAAG+AGG + chr7.1:48791820-48791839 MS.gene027327:CDS 40.0%
CAGTGGCCAATTTCATAGAT+AGG - chr7.1:48792611-48792630 None:intergenic 40.0%
CTCAATACTAAAATCCACCC+TGG - chr7.1:48792640-48792659 None:intergenic 40.0%
GATGAAAGTGATTGCCATGT+AGG - chr7.1:48790826-48790845 None:intergenic 40.0%
GCTGCAAAATTCTCTTGCTT+TGG + chr7.1:48791207-48791226 MS.gene027327:CDS 40.0%
GGCATAAACCACCATCTAAT+GGG - chr7.1:48792937-48792956 None:intergenic 40.0%
TACCAACCTTATTGCAACCA+GGG - chr7.1:48792190-48792209 None:intergenic 40.0%
TGGCATAAACCACCATCTAA+TGG - chr7.1:48792938-48792957 None:intergenic 40.0%
TGTTGTTGCCCATAATAGGA+TGG - chr7.1:48792851-48792870 None:intergenic 40.0%
TTACCAACCTTATTGCAACC+AGG - chr7.1:48792191-48792210 None:intergenic 40.0%
! ATAAGTGCATGCCAAGGTTT+TGG - chr7.1:48792958-48792977 None:intergenic 40.0%
! ATGGTGCTGAGGAATCTAAT+GGG - chr7.1:48792832-48792851 None:intergenic 40.0%
! ATGTTGGAATTTGAAGCTGC+AGG - chr7.1:48790955-48790974 None:intergenic 40.0%
! TGCTTTTCCAGTAACCTAGA+AGG - chr7.1:48792289-48792308 None:intergenic 40.0%
AACTCAGTGGACCCATTAGA+TGG + chr7.1:48792923-48792942 MS.gene027327:CDS 45.0%
AATTGGCCACTGTTAACACC+AGG + chr7.1:48792619-48792638 MS.gene027327:CDS 45.0%
AGCAGAGGAAGAGTCAAAAG+GGG + chr7.1:48791720-48791739 MS.gene027327:CDS 45.0%
ATTGGCCACTGTTAACACCA+GGG + chr7.1:48792620-48792639 MS.gene027327:CDS 45.0%
CCTCAGCACCATCCTATTAT+GGG + chr7.1:48792840-48792859 MS.gene027327:CDS 45.0%
CTTCTACAAGATCTTGTCCC+TGG + chr7.1:48792170-48792189 MS.gene027327:CDS 45.0%
GCAGAATTCTCAACTGATCC+TGG + chr7.1:48791171-48791190 MS.gene027327:CDS 45.0%
GGAGGGAGAAAATCAGTGAA+AGG + chr7.1:48792141-48792160 MS.gene027327:CDS 45.0%
GGTGGTTTATGCCAAAACCT+TGG + chr7.1:48792944-48792963 MS.gene027327:CDS 45.0%
GTGAAGATAAGTGCATGCCA+AGG - chr7.1:48792964-48792983 None:intergenic 45.0%
TCCTCAGCACCATCCTATTA+TGG + chr7.1:48792839-48792858 MS.gene027327:CDS 45.0%
TCTTGTCCCTGGTTGCAATA+AGG + chr7.1:48792181-48792200 MS.gene027327:CDS 45.0%
! ATCATTGCAGCGTCAAGTTG+AGG + chr7.1:48792341-48792360 MS.gene027327:CDS 45.0%
! GATGGTGCTGAGGAATCTAA+TGG - chr7.1:48792833-48792852 None:intergenic 45.0%
! TAATGGGTCCACTGAGTTGT+GGG - chr7.1:48792921-48792940 None:intergenic 45.0%
! TGTGAATTGCTGGGTTTTGC+TGG - chr7.1:48792874-48792893 None:intergenic 45.0%
!! ATGTAATCCTTTGGTGCCTC+AGG - chr7.1:48791802-48791821 None:intergenic 45.0%
!! CTGGTGTTGTTGCCCATAAT+AGG - chr7.1:48792855-48792874 None:intergenic 45.0%
AACTGTGACCCACAACTCAG+TGG + chr7.1:48792910-48792929 MS.gene027327:CDS 50.0%
GAGCAACTCAAAACCTCCTG+AGG + chr7.1:48791783-48791802 MS.gene027327:CDS 50.0%
GCAGAGGAAGAGTCAAAAGG+GGG + chr7.1:48791721-48791740 MS.gene027327:CDS 50.0%
GCAGCTCTTTCTGCAAAACC+AGG - chr7.1:48791192-48791211 None:intergenic 50.0%
GTCCCTGGTTGCAATAAGGT+TGG + chr7.1:48792185-48792204 MS.gene027327:intron 50.0%
TACGACCGCAATTGCAGTTG+CGG + chr7.1:48792035-48792054 MS.gene027327:intron 50.0%
TCAGTGGACCCATTAGATGG+TGG + chr7.1:48792926-48792945 MS.gene027327:CDS 50.0%
! AAAACCTCCTGAGGCACCAA+AGG + chr7.1:48791792-48791811 MS.gene027327:CDS 50.0%
! ATCCACCCTGGTGTTAACAG+TGG - chr7.1:48792628-48792647 None:intergenic 50.0%
! CCCATAATAGGATGGTGCTG+AGG - chr7.1:48792843-48792862 None:intergenic 50.0%
! CTAATGGGTCCACTGAGTTG+TGG - chr7.1:48792922-48792941 None:intergenic 50.0%
!! TAATCCTTTGGTGCCTCAGG+AGG - chr7.1:48791799-48791818 None:intergenic 50.0%
GTGGTCCGCAACTGCAATTG+CGG - chr7.1:48792043-48792062 None:intergenic 55.0%
GGCCACTGTTAACACCAGGG+TGG + chr7.1:48792623-48792642 MS.gene027327:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 48790820 48793012 48790820 ID=MS.gene027327
chr7.1 mRNA 48790820 48793012 48790820 ID=MS.gene027327.t1;Parent=MS.gene027327
chr7.1 exon 48790820 48791536 48790820 ID=MS.gene027327.t1.exon1;Parent=MS.gene027327.t1
chr7.1 CDS 48790820 48791536 48790820 ID=cds.MS.gene027327.t1;Parent=MS.gene027327.t1
chr7.1 exon 48791643 48791876 48791643 ID=MS.gene027327.t1.exon2;Parent=MS.gene027327.t1
chr7.1 CDS 48791643 48791876 48791643 ID=cds.MS.gene027327.t1;Parent=MS.gene027327.t1
chr7.1 exon 48792137 48792202 48792137 ID=MS.gene027327.t1.exon3;Parent=MS.gene027327.t1
chr7.1 CDS 48792137 48792202 48792137 ID=cds.MS.gene027327.t1;Parent=MS.gene027327.t1
chr7.1 exon 48792294 48792362 48792294 ID=MS.gene027327.t1.exon4;Parent=MS.gene027327.t1
chr7.1 CDS 48792294 48792362 48792294 ID=cds.MS.gene027327.t1;Parent=MS.gene027327.t1
chr7.1 exon 48792606 48792677 48792606 ID=MS.gene027327.t1.exon5;Parent=MS.gene027327.t1
chr7.1 CDS 48792606 48792677 48792606 ID=cds.MS.gene027327.t1;Parent=MS.gene027327.t1
chr7.1 exon 48792791 48793012 48792791 ID=MS.gene027327.t1.exon6;Parent=MS.gene027327.t1
chr7.1 CDS 48792791 48793012 48792791 ID=cds.MS.gene027327.t1;Parent=MS.gene027327.t1
Gene Sequence

>MS.gene027327

ATGCCTACATGGCAATCACTTTCATCAGAACAACAGAACCAAGATTGTTTTTACACACCAATATGGGATAAGTCAACAACAGATCAATGTGTTAATCAATTTGATTCAGCACTTAGTTCAATGGTATCATCACCTGCAGCTTCAAATTCCAACATGTCAAATGACAATTTTGTCATCAGAGAATTGATTGGTAAATTAGGAAACATTGGTAATTGTTCTGATGAGATCTCACAAACATTGGTTGCTGCAAATTCATACATCAATAGCAATAACAATACTTCATGTTACAACACCCCTTTGAATTCTCCTCCAAAATTGAACATCTCAACAATGGCCATGAATTCAACTGTTGCAGAATTCTCAACTGATCCTGGTTTTGCAGAAAGAGCTGCAAAATTCTCTTGCTTTGGAAGTAGGAGTTTCAATGGAAGAACAACTCAATTGAGTCAAAGTCAAAGATCTACTCCATTATTGGAAAATGGAAAACTCTCTAGAGTTTCAAGTAGTCCATCATTGAAAGAACTTGGATCTCAATCTCAAGAGAATATGAATTGTTCTACTCAATTGCAGGATCAAATTGAAGTTAACAATTCTCAAGAAGAATCAACAATCTCTGAAAATGGTGTGAAACCTTCTCCTTATGTGAATTCAAGGAAAAGAAAAGTTTCTTCCAAAGGAAAAACCAAAGAAACTTCAACTTCTAGTAACCCTGCAATGGTAAGTTAAAAATTGAATTTTTACTTTATTTTATTTGACCCTCTTAATTAAATTAGTCATTTCATATTCTAAATCCAAAAGGGTCATTTTTGTTTTTGCAATATAGGCTTGTGAAGCTGAAGACTCAAATGCAAAGCGCATGAAAACAAATGAAGGTGAAAAAATTGAAAATGGTAAAGTAAAAGCAGAGGAAGAGTCAAAAGGGGGTACAAATAGTAATAATGGAGGTGATGAAAAACAGAATAAGAGCAACTCAAAACCTCCTGAGGCACCAAAGGATTACATTCATGTCAGAGCAAGAAGAGGTCAAGCTACTGATAGCCATAGTCTTGCAGAAAGAGTAAGAATTTTCATTGTCATTTTCATTCCATAATTGACACTAACAATCATAGTTTTAAATTGCGATTACGCTGCAAACCTTTATATTGCCACAATATGTGACAATGTGACAAAATGCAAAAAAAATTGTTGTCGCGGAGACCTCAAAATATGCTATAATACGACCGCAATTGCAGTTGCGGACCACAATTTAAAACCATGATGGCAATATACATGTTAGGCTTTCAATTTTGACATTGTGTAAACTAATTTTTGTTATAGGTTAGGAGGGAGAAAATCAGTGAAAGGATGAAGCTTCTACAAGATCTTGTCCCTGGTTGCAATAAGGTTGGTAATACTTAGATAAATTAAGGATTCATAATTTGATTGGTTAGTTTATATTTACTAATTTTTTGTTATGAATTATTATTCCTTCTAGGTTACTGGAAAAGCACTCATGCTTGATGAAATTATAAACTATGTGCAATCATTGCAGCGTCAAGTTGAGGTAAGATAATGATCTTTGAAATTATAAAAAAATTACTGCATGCATACTAATTTTATTTGGTTAAAATAATATATGATAAAATGCTTTAAAAATTTATCAACCTTAAAAATTACATGACTTTTTTTTTTTTTTTTATATTTAAAATTGGAAGGAATAAGCATATTATCACATGATGATAGTTATAATGTTAAGATTTCTTGCTCATTAAGTTTTTATTTTATTGTTTGAATTTTTTGTTTGCAGTTCCTATCTATGAAATTGGCCACTGTTAACACCAGGGTGGATTTTAGTATTGAGAGTCTAATCTCAAAAGATGTAAGTTTAAGGGCTCTAGATAATGGATTTGCTTTTTAATAGATAAATAAAAAGTATTGCATTTTTTTATTAACTTCAATTTGTTGACTTTATATTTTTTTTTGTAATTTCAGATGTTTCAATCAAATAATTCTTTGGCACACCCAATATTCCCATTAGATTCCTCAGCACCATCCTATTATGGGCAACAACACCAGCAAAACCCAGCAATTCACAATAATATTTCTAATGGAACTGTGACCCACAACTCAGTGGACCCATTAGATGGTGGTTTATGCCAAAACCTTGGCATGCACTTATCTTCACTAAGTGGATTTAATGAAGCTGGCTCACAG
Protein sequence

>MS.gene027327.t1

MPTWQSLSSEQQNQDCFYTPIWDKSTTDQCVNQFDSALSSMVSSPAASNSNMSNDNFVIRELIGKLGNIGNCSDEISQTLVAANSYINSNNNTSCYNTPLNSPPKLNISTMAMNSTVAEFSTDPGFAERAAKFSCFGSRSFNGRTTQLSQSQRSTPLLENGKLSRVSSSPSLKELGSQSQENMNCSTQLQDQIEVNNSQEESTISENGVKPSPYVNSRKRKVSSKGKTKETSTSSNPAMACEAEDSNAKRMKTNEGEKIENGKVKAEEESKGGTNSNNGGDEKQNKSNSKPPEAPKDYIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGCNKVTGKALMLDEIINYVQSLQRQVEFLSMKLATVNTRVDFSIESLISKDMFQSNNSLAHPIFPLDSSAPSYYGQQHQQNPAIHNNISNGTVTHNSVDPLDGGLCQNLGMHLSSLSGFNEAGSQ