AlfalfaGEDB | MS.gene027327

M. sativa cultivar XinJiangDaYe / MS.gene027327

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027327.t1	XP_013448523.1	95.3	464	12	2	1	460	19	476	3.40E-225	790.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027327.t1	Q9SRT2	45.3	468	170	12	1	460	26	415	5.3e-78	293.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027327.t1	A0A072U9Q4	95.3	464	12	2	1	460	19	476	2.4e-225	790.8

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene027327.t1	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027327.t1	MTR_7g053410	96.552	464	6	2	1	460	19	476	0.0	910
MS.gene027327.t1	MTR_7g053410	96.552	464	6	2	1	460	19	476	0.0	909
MS.gene027327.t1	MTR_8g012290	62.791	473	116	14	12	460	15	451	2.25e-175	501
MS.gene027327.t1	MTR_8g012290	62.791	473	116	14	12	460	15	451	3.51e-175	500
MS.gene027327.t1	MTR_1g017350	36.979	384	169	11	79	426	74	420	2.29e-54	187
MS.gene027327.t1	MTR_1g017350	36.788	386	171	11	79	428	74	422	2.60e-54	187
MS.gene027327.t1	MTR_1g017350	37.008	381	170	11	79	426	74	417	4.08e-53	184
MS.gene027327.t1	MTR_1g017350	36.815	383	172	11	79	428	74	419	6.12e-53	184
MS.gene027327.t1	MTR_8g065740	38.310	355	163	13	116	440	125	453	1.58e-52	184
MS.gene027327.t1	MTR_4g070320	58.235	170	56	4	246	408	85	246	1.22e-51	178

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027327.t1	AT3G07340	45.824	467	169	12	1	460	26	415	7.38e-112	337
MS.gene027327.t1	AT3G07340	45.824	467	169	12	1	460	26	415	9.14e-112	337
MS.gene027327.t1	AT5G48560	47.433	487	177	20	1	460	32	466	3.23e-98	303
MS.gene027327.t1	AT5G48560	47.433	487	177	20	1	460	32	466	1.11e-97	302
MS.gene027327.t1	AT3G23690	57.071	198	73	4	216	409	113	302	1.34e-59	200
MS.gene027327.t1	AT1G68920	43.284	335	142	13	96	400	97	413	1.42e-58	200
MS.gene027327.t1	AT1G68920	41.493	335	149	10	96	400	97	414	2.27e-58	199
MS.gene027327.t1	AT1G68920	41.493	335	149	10	96	400	97	414	2.27e-58	199
MS.gene027327.t1	AT1G68920	41.493	335	149	10	96	400	97	414	2.27e-58	199
MS.gene027327.t1	AT1G26260	42.088	297	119	10	111	400	68	318	2.72e-53	184

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Find 83 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCTCCTGAGGCACCAA+AGG	0.566429	7.1:+48791792	MS.gene027327:CDS
AAAAGAAAAGTTTCTTCCAA+AGG	0.532200	7.1:+48791474	MS.gene027327:CDS
AAAATTGAACATCTCAACAA+TGG	0.629044	7.1:+48791131	MS.gene027327:CDS
AAAATTGTCATTTGACATGT+TGG	0.469622	7.1:-48790968	None:intergenic
AAAGCAGAGGAAGAGTCAAA+AGG	0.521509	7.1:+48791718	MS.gene027327:CDS
AAATATTATTGTGAATTGCT+GGG	0.490799	7.1:-48792880	None:intergenic
AAATGGTAAAGTAAAAGCAG+AGG	0.646618	7.1:+48791705	MS.gene027327:CDS
AACCATCTTCAATGCCTACA+TGG	0.550038	7.1:+48790809	None:intergenic
AACTCAGTGGACCCATTAGA+TGG	0.450062	7.1:+48792923	MS.gene027327:CDS
AACTGTGACCCACAACTCAG+TGG	0.673850	7.1:+48792910	MS.gene027327:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATGGTAAAGTAAAAGCAG+AGG	+	chr7.1:48791705-48791724	MS.gene027327:CDS	30.0%
ACTAGAAGTTGAAGTTTCTT+TGG	-	chr7.1:48791505-48791524	None:intergenic	30.0%
AGGAATCTAATGGGAATATT+GGG	-	chr7.1:48792823-48792842	None:intergenic	30.0%
ATGATGGCAATATACATGTT+AGG	+	chr7.1:48792074-48792093	MS.gene027327:intron	30.0%
CACATATTGTGGCAATATAA+AGG	-	chr7.1:48791957-48791976	None:intergenic	30.0%
GGTACAAATAGTAATAATGG+AGG	+	chr7.1:48791742-48791761	MS.gene027327:CDS	30.0%
TAACATGTATATTGCCATCA+TGG	-	chr7.1:48792075-48792094	None:intergenic	30.0%
TATTATTCCTTCTAGGTTAC+TGG	+	chr7.1:48792279-48792298	MS.gene027327:intron	30.0%
TCAAAGATCTACTCCATTAT+TGG	+	chr7.1:48791272-48791291	MS.gene027327:CDS	30.0%
TCTACTCCATTATTGGAAAA+TGG	+	chr7.1:48791279-48791298	MS.gene027327:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	48790820	48793012	48790820	ID=MS.gene027327
chr7.1	mRNA	48790820	48793012	48790820	ID=MS.gene027327.t1;Parent=MS.gene027327
chr7.1	exon	48790820	48791536	48790820	ID=MS.gene027327.t1.exon1;Parent=MS.gene027327.t1
chr7.1	CDS	48790820	48791536	48790820	ID=cds.MS.gene027327.t1;Parent=MS.gene027327.t1
chr7.1	exon	48791643	48791876	48791643	ID=MS.gene027327.t1.exon2;Parent=MS.gene027327.t1
chr7.1	CDS	48791643	48791876	48791643	ID=cds.MS.gene027327.t1;Parent=MS.gene027327.t1
chr7.1	exon	48792137	48792202	48792137	ID=MS.gene027327.t1.exon3;Parent=MS.gene027327.t1
chr7.1	CDS	48792137	48792202	48792137	ID=cds.MS.gene027327.t1;Parent=MS.gene027327.t1
chr7.1	exon	48792294	48792362	48792294	ID=MS.gene027327.t1.exon4;Parent=MS.gene027327.t1
chr7.1	CDS	48792294	48792362	48792294	ID=cds.MS.gene027327.t1;Parent=MS.gene027327.t1
chr7.1	exon	48792606	48792677	48792606	ID=MS.gene027327.t1.exon5;Parent=MS.gene027327.t1
chr7.1	CDS	48792606	48792677	48792606	ID=cds.MS.gene027327.t1;Parent=MS.gene027327.t1
chr7.1	exon	48792791	48793012	48792791	ID=MS.gene027327.t1.exon6;Parent=MS.gene027327.t1
chr7.1	CDS	48792791	48793012	48792791	ID=cds.MS.gene027327.t1;Parent=MS.gene027327.t1

Gene Sequence

>MS.gene027327

ATGCCTACATGGCAATCACTTTCATCAGAACAACAGAACCAAGATTGTTTTTACACACCAATATGGGATAAGTCAACAACAGATCAATGTGTTAATCAATTTGATTCAGCACTTAGTTCAATGGTATCATCACCTGCAGCTTCAAATTCCAACATGTCAAATGACAATTTTGTCATCAGAGAATTGATTGGTAAATTAGGAAACATTGGTAATTGTTCTGATGAGATCTCACAAACATTGGTTGCTGCAAATTCATACATCAATAGCAATAACAATACTTCATGTTACAACACCCCTTTGAATTCTCCTCCAAAATTGAACATCTCAACAATGGCCATGAATTCAACTGTTGCAGAATTCTCAACTGATCCTGGTTTTGCAGAAAGAGCTGCAAAATTCTCTTGCTTTGGAAGTAGGAGTTTCAATGGAAGAACAACTCAATTGAGTCAAAGTCAAAGATCTACTCCATTATTGGAAAATGGAAAACTCTCTAGAGTTTCAAGTAGTCCATCATTGAAAGAACTTGGATCTCAATCTCAAGAGAATATGAATTGTTCTACTCAATTGCAGGATCAAATTGAAGTTAACAATTCTCAAGAAGAATCAACAATCTCTGAAAATGGTGTGAAACCTTCTCCTTATGTGAATTCAAGGAAAAGAAAAGTTTCTTCCAAAGGAAAAACCAAAGAAACTTCAACTTCTAGTAACCCTGCAATGGTAAGTTAAAAATTGAATTTTTACTTTATTTTATTTGACCCTCTTAATTAAATTAGTCATTTCATATTCTAAATCCAAAAGGGTCATTTTTGTTTTTGCAATATAGGCTTGTGAAGCTGAAGACTCAAATGCAAAGCGCATGAAAACAAATGAAGGTGAAAAAATTGAAAATGGTAAAGTAAAAGCAGAGGAAGAGTCAAAAGGGGGTACAAATAGTAATAATGGAGGTGATGAAAAACAGAATAAGAGCAACTCAAAACCTCCTGAGGCACCAAAGGATTACATTCATGTCAGAGCAAGAAGAGGTCAAGCTACTGATAGCCATAGTCTTGCAGAAAGAGTAAGAATTTTCATTGTCATTTTCATTCCATAATTGACACTAACAATCATAGTTTTAAATTGCGATTACGCTGCAAACCTTTATATTGCCACAATATGTGACAATGTGACAAAATGCAAAAAAAATTGTTGTCGCGGAGACCTCAAAATATGCTATAATACGACCGCAATTGCAGTTGCGGACCACAATTTAAAACCATGATGGCAATATACATGTTAGGCTTTCAATTTTGACATTGTGTAAACTAATTTTTGTTATAGGTTAGGAGGGAGAAAATCAGTGAAAGGATGAAGCTTCTACAAGATCTTGTCCCTGGTTGCAATAAGGTTGGTAATACTTAGATAAATTAAGGATTCATAATTTGATTGGTTAGTTTATATTTACTAATTTTTTGTTATGAATTATTATTCCTTCTAGGTTACTGGAAAAGCACTCATGCTTGATGAAATTATAAACTATGTGCAATCATTGCAGCGTCAAGTTGAGGTAAGATAATGATCTTTGAAATTATAAAAAAATTACTGCATGCATACTAATTTTATTTGGTTAAAATAATATATGATAAAATGCTTTAAAAATTTATCAACCTTAAAAATTACATGACTTTTTTTTTTTTTTTTATATTTAAAATTGGAAGGAATAAGCATATTATCACATGATGATAGTTATAATGTTAAGATTTCTTGCTCATTAAGTTTTTATTTTATTGTTTGAATTTTTTGTTTGCAGTTCCTATCTATGAAATTGGCCACTGTTAACACCAGGGTGGATTTTAGTATTGAGAGTCTAATCTCAAAAGATGTAAGTTTAAGGGCTCTAGATAATGGATTTGCTTTTTAATAGATAAATAAAAAGTATTGCATTTTTTTATTAACTTCAATTTGTTGACTTTATATTTTTTTTTGTAATTTCAGATGTTTCAATCAAATAATTCTTTGGCACACCCAATATTCCCATTAGATTCCTCAGCACCATCCTATTATGGGCAACAACACCAGCAAAACCCAGCAATTCACAATAATATTTCTAATGGAACTGTGACCCACAACTCAGTGGACCCATTAGATGGTGGTTTATGCCAAAACCTTGGCATGCACTTATCTTCACTAAGTGGATTTAATGAAGCTGGCTCACAG

Protein sequence

>MS.gene027327.t1

MPTWQSLSSEQQNQDCFYTPIWDKSTTDQCVNQFDSALSSMVSSPAASNSNMSNDNFVIRELIGKLGNIGNCSDEISQTLVAANSYINSNNNTSCYNTPLNSPPKLNISTMAMNSTVAEFSTDPGFAERAAKFSCFGSRSFNGRTTQLSQSQRSTPLLENGKLSRVSSSPSLKELGSQSQENMNCSTQLQDQIEVNNSQEESTISENGVKPSPYVNSRKRKVSSKGKTKETSTSSNPAMACEAEDSNAKRMKTNEGEKIENGKVKAEEESKGGTNSNNGGDEKQNKSNSKPPEAPKDYIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGCNKVTGKALMLDEIINYVQSLQRQVEFLSMKLATVNTRVDFSIESLISKDMFQSNNSLAHPIFPLDSSAPSYYGQQHQQNPAIHNNISNGTVTHNSVDPLDGGLCQNLGMHLSSLSGFNEAGSQ