Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027338.t1 | XP_013468829.1 | 91.2 | 159 | 14 | 0 | 1 | 159 | 1 | 159 | 1.10E-71 | 279.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027338.t1 | Q9FJK3 | 44.9 | 156 | 85 | 1 | 1 | 155 | 1 | 156 | 1.3e-26 | 120.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027338.t1 | A0A072VMQ1 | 91.2 | 159 | 14 | 0 | 1 | 159 | 1 | 159 | 8.3e-72 | 279.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene027338.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027338.t1 | MTR_1g077320 | 91.195 | 159 | 14 | 0 | 1 | 159 | 1 | 159 | 1.40e-96 | 276 |
| MS.gene027338.t1 | MTR_4g028720 | 85.535 | 159 | 23 | 0 | 1 | 159 | 1 | 159 | 3.53e-92 | 265 |
| MS.gene027338.t1 | MTR_7g011950 | 87.421 | 159 | 20 | 0 | 1 | 159 | 1 | 159 | 4.85e-92 | 264 |
| MS.gene027338.t1 | MTR_1g077390 | 70.064 | 157 | 47 | 0 | 1 | 157 | 1 | 157 | 6.40e-77 | 226 |
| MS.gene027338.t1 | MTR_1g077300 | 73.585 | 159 | 40 | 1 | 1 | 159 | 1 | 157 | 2.01e-73 | 217 |
| MS.gene027338.t1 | MTR_5g047580 | 67.949 | 156 | 50 | 0 | 1 | 156 | 1 | 156 | 9.73e-73 | 215 |
| MS.gene027338.t1 | MTR_1g084950 | 71.795 | 156 | 44 | 0 | 1 | 156 | 1 | 156 | 3.54e-71 | 211 |
| MS.gene027338.t1 | MTR_1g090697 | 56.774 | 155 | 67 | 0 | 1 | 155 | 1 | 155 | 7.97e-58 | 177 |
| MS.gene027338.t1 | MTR_1g090710 | 55.128 | 156 | 69 | 1 | 1 | 155 | 1 | 156 | 2.03e-55 | 171 |
| MS.gene027338.t1 | MTR_1g090783 | 54.902 | 153 | 69 | 0 | 3 | 155 | 2 | 154 | 5.39e-55 | 170 |
| MS.gene027338.t1 | MTR_5g047560 | 57.051 | 156 | 51 | 1 | 1 | 156 | 1 | 140 | 1.32e-54 | 169 |
| MS.gene027338.t1 | MTR_5g075380 | 39.103 | 156 | 94 | 1 | 1 | 155 | 1 | 156 | 9.40e-38 | 127 |
| MS.gene027338.t1 | MTR_3g065100 | 49.045 | 157 | 79 | 1 | 1 | 156 | 1 | 157 | 1.17e-37 | 129 |
| MS.gene027338.t1 | MTR_3g466980 | 43.137 | 153 | 86 | 1 | 1 | 152 | 1 | 153 | 5.83e-36 | 124 |
| MS.gene027338.t1 | MTR_4g032620 | 38.065 | 155 | 94 | 2 | 1 | 153 | 1 | 155 | 4.01e-35 | 122 |
| MS.gene027338.t1 | MTR_3g466830 | 42.675 | 157 | 89 | 1 | 3 | 158 | 2 | 158 | 2.33e-33 | 115 |
| MS.gene027338.t1 | MTR_4g031910 | 44.000 | 150 | 82 | 2 | 1 | 148 | 1 | 150 | 4.90e-33 | 117 |
| MS.gene027338.t1 | MTR_4g063790 | 38.365 | 159 | 96 | 2 | 1 | 157 | 1 | 159 | 1.20e-32 | 114 |
| MS.gene027338.t1 | MTR_3g031240 | 37.107 | 159 | 98 | 2 | 1 | 157 | 1 | 159 | 1.39e-31 | 112 |
| MS.gene027338.t1 | MTR_3g466890 | 40.909 | 154 | 90 | 1 | 3 | 155 | 2 | 155 | 1.95e-30 | 108 |
| MS.gene027338.t1 | MTR_8g036130 | 37.736 | 159 | 95 | 2 | 1 | 155 | 1 | 159 | 5.38e-29 | 107 |
| MS.gene027338.t1 | MTR_4g032260 | 41.875 | 160 | 91 | 2 | 1 | 158 | 1 | 160 | 1.97e-28 | 103 |
| MS.gene027338.t1 | MTR_2g016210 | 38.994 | 159 | 95 | 2 | 1 | 157 | 1 | 159 | 1.70e-27 | 103 |
| MS.gene027338.t1 | MTR_4g032290 | 40.994 | 161 | 93 | 2 | 1 | 159 | 1 | 161 | 5.15e-27 | 101 |
| MS.gene027338.t1 | MTR_3g467080 | 32.919 | 161 | 106 | 2 | 1 | 159 | 1 | 161 | 6.98e-26 | 96.7 |
| MS.gene027338.t1 | MTR_3g031100 | 35.443 | 158 | 98 | 3 | 1 | 155 | 1 | 157 | 2.42e-25 | 97.4 |
| MS.gene027338.t1 | MTR_2g035610 | 42.735 | 117 | 66 | 1 | 1 | 116 | 1 | 117 | 8.18e-25 | 92.8 |
| MS.gene027338.t1 | MTR_2g035580 | 31.613 | 155 | 105 | 1 | 1 | 154 | 1 | 155 | 7.48e-20 | 81.3 |
| MS.gene027338.t1 | MTR_3g465410 | 34.351 | 131 | 84 | 2 | 29 | 157 | 1 | 131 | 9.30e-20 | 80.5 |
| MS.gene027338.t1 | MTR_4g028800 | 46.575 | 73 | 37 | 1 | 1 | 73 | 1 | 71 | 6.64e-19 | 76.3 |
| MS.gene027338.t1 | MTR_3g466900 | 38.095 | 126 | 75 | 2 | 32 | 155 | 2 | 126 | 4.27e-18 | 75.9 |
| MS.gene027338.t1 | MTR_3g466930 | 36.000 | 125 | 79 | 1 | 32 | 155 | 2 | 126 | 5.82e-18 | 75.5 |
| MS.gene027338.t1 | MTR_4g019670 | 35.000 | 120 | 74 | 2 | 1 | 116 | 1 | 120 | 6.69e-16 | 74.3 |
| MS.gene027338.t1 | MTR_7g106510 | 33.636 | 110 | 72 | 1 | 1 | 109 | 4 | 113 | 3.95e-15 | 72.0 |
| MS.gene027338.t1 | MTR_7g055800 | 33.333 | 114 | 49 | 2 | 1 | 114 | 1 | 87 | 7.85e-14 | 63.5 |
| MS.gene027338.t1 | MTR_6g005450 | 30.973 | 113 | 70 | 3 | 1 | 106 | 1 | 112 | 5.14e-12 | 62.8 |
| MS.gene027338.t1 | MTR_7g055790 | 37.615 | 109 | 51 | 2 | 1 | 109 | 59 | 150 | 8.36e-12 | 60.1 |
| MS.gene027338.t1 | MTR_7g055940 | 34.286 | 105 | 42 | 3 | 1 | 105 | 1 | 78 | 1.01e-11 | 57.8 |
| MS.gene027338.t1 | MTR_1g033370 | 40.426 | 94 | 55 | 1 | 64 | 156 | 28 | 121 | 1.90e-11 | 58.2 |
| MS.gene027338.t1 | MTR_1g033930 | 37.500 | 96 | 59 | 1 | 64 | 158 | 22 | 117 | 2.57e-11 | 57.8 |
| MS.gene027338.t1 | MTR_3g093900 | 26.316 | 152 | 107 | 2 | 3 | 151 | 6 | 155 | 4.68e-11 | 60.1 |
| MS.gene027338.t1 | MTR_1g033790 | 38.542 | 96 | 58 | 1 | 64 | 158 | 22 | 117 | 9.61e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027338.t1 | AT5G48670 | 44.872 | 156 | 85 | 1 | 1 | 155 | 1 | 156 | 1.06e-31 | 115 |
| MS.gene027338.t1 | AT3G05860 | 39.597 | 149 | 89 | 1 | 1 | 148 | 1 | 149 | 1.47e-30 | 110 |
| MS.gene027338.t1 | AT3G05860 | 39.597 | 149 | 89 | 1 | 1 | 148 | 1 | 149 | 7.11e-30 | 109 |
| MS.gene027338.t1 | AT5G26630 | 39.375 | 160 | 96 | 1 | 1 | 159 | 1 | 160 | 8.75e-30 | 108 |
| MS.gene027338.t1 | AT3G05860 | 39.597 | 149 | 89 | 1 | 1 | 148 | 1 | 149 | 1.06e-29 | 109 |
| MS.gene027338.t1 | AT1G65300 | 35.417 | 144 | 92 | 1 | 3 | 145 | 2 | 145 | 2.88e-25 | 98.2 |
| MS.gene027338.t1 | AT1G65330 | 35.374 | 147 | 94 | 1 | 3 | 148 | 2 | 148 | 9.22e-25 | 96.7 |
| MS.gene027338.t1 | AT5G27960 | 43.925 | 107 | 59 | 1 | 4 | 109 | 2 | 108 | 6.50e-23 | 92.4 |
| MS.gene027338.t1 | AT5G26650 | 43.925 | 107 | 59 | 1 | 4 | 109 | 2 | 108 | 7.60e-23 | 93.2 |
| MS.gene027338.t1 | AT1G22590 | 34.711 | 121 | 66 | 2 | 1 | 114 | 1 | 115 | 3.16e-20 | 82.4 |
| MS.gene027338.t1 | AT5G27810 | 44.186 | 86 | 47 | 1 | 29 | 113 | 1 | 86 | 4.33e-19 | 78.2 |
| MS.gene027338.t1 | AT1G31630 | 34.234 | 111 | 71 | 2 | 3 | 112 | 2 | 111 | 8.91e-19 | 81.6 |
| MS.gene027338.t1 | AT2G28700 | 36.364 | 110 | 69 | 1 | 1 | 109 | 1 | 110 | 3.92e-18 | 79.7 |
| MS.gene027338.t1 | AT1G31640 | 39.640 | 111 | 65 | 2 | 3 | 112 | 2 | 111 | 5.34e-18 | 80.1 |
| MS.gene027338.t1 | AT5G26580 | 39.450 | 109 | 53 | 1 | 1 | 109 | 1 | 96 | 5.42e-17 | 76.6 |
| MS.gene027338.t1 | AT2G40210 | 38.835 | 103 | 58 | 2 | 1 | 100 | 1 | 101 | 1.26e-13 | 67.4 |
| MS.gene027338.t1 | AT5G58890 | 35.135 | 111 | 64 | 3 | 1 | 104 | 1 | 110 | 1.47e-13 | 67.0 |
| MS.gene027338.t1 | AT5G06500 | 35.849 | 106 | 64 | 2 | 1 | 102 | 1 | 106 | 1.28e-11 | 60.8 |
Find 29 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGCAAAAGGAAACTTTCT+TGG | 0.253590 | 7.4:-49887392 | None:intergenic |
| CTTGTCTTTGCTTTCTCAGC+TGG | 0.304732 | 7.4:-49887434 | None:intergenic |
| TGTTGAATCATTGGAGATAA+AGG | 0.341938 | 7.4:-49887166 | None:intergenic |
| AACAGAGGTGTGGCCAAATC+TGG | 0.342460 | 7.4:+49887302 | MS.gene027338:CDS |
| AGCCTTTCTTGTTGAATCAT+TGG | 0.353862 | 7.4:-49887175 | None:intergenic |
| CTCCAATGATTCAACAAGAA+AGG | 0.407412 | 7.4:+49887173 | MS.gene027338:CDS |
| AAATCTGGAGGAAGCAAAAC+AGG | 0.418438 | 7.4:+49887317 | MS.gene027338:CDS |
| CTTTAGTAATCTGTTGCAAA+AGG | 0.444882 | 7.4:-49887404 | None:intergenic |
| TCTTTCTAATTTGAAAGACT+TGG | 0.450624 | 7.4:+49887530 | MS.gene027338:CDS |
| CAGAACTCATATATGAAAGA+TGG | 0.456034 | 7.4:+49887354 | MS.gene027338:CDS |
| ACCTCTGTTTCAAAATCAAA+AGG | 0.463838 | 7.4:-49887288 | None:intergenic |
| TTAGAAAGAGTCATATCATC+TGG | 0.478048 | 7.4:-49887516 | None:intergenic |
| TTTCACCTTCTTCCTAGTCA+TGG | 0.490416 | 7.4:-49887139 | None:intergenic |
| AGAGAACTCACTATTCTTTG+TGG | 0.526508 | 7.4:+49887237 | MS.gene027338:CDS |
| TGCAACAGATTACTAAAGCT+AGG | 0.531119 | 7.4:+49887409 | MS.gene027338:CDS |
| GCAACAGATTACTAAAGCTA+GGG | 0.532673 | 7.4:+49887410 | MS.gene027338:CDS |
| AGAACTCATATATGAAAGAT+GGG | 0.538835 | 7.4:+49887355 | MS.gene027338:CDS |
| AGCTGAACATTCGTATGAGT+TGG | 0.547236 | 7.4:+49887472 | MS.gene027338:CDS |
| GAAGGAAATTGATGACAAGA+TGG | 0.550366 | 7.4:+49887575 | MS.gene027338:CDS |
| CGAAGCCATGACTAGGAAGA+AGG | 0.551367 | 7.4:+49887134 | None:intergenic |
| GAAGAAAAGTATCATCAAGA+AGG | 0.556586 | 7.4:+49887212 | MS.gene027338:CDS |
| AGGATCGGAAATTATAGCAC+AGG | 0.559456 | 7.4:-49887268 | None:intergenic |
| GAAAGGCTTCCTACAAGAAG+AGG | 0.563850 | 7.4:+49887190 | MS.gene027338:CDS |
| TCGGAAATTATAGCACAGGC+TGG | 0.570119 | 7.4:-49887264 | None:intergenic |
| TGTTTCAAAATCAAAAGGAT+CGG | 0.583002 | 7.4:-49887283 | None:intergenic |
| TACATTGCGAAGCCATGACT+AGG | 0.618148 | 7.4:+49887127 | None:intergenic |
| AAGCAAAACAGGTGATTGAG+AGG | 0.635256 | 7.4:+49887328 | MS.gene027338:CDS |
| AGAGGTGTGGCCAAATCTGG+AGG | 0.647190 | 7.4:+49887305 | MS.gene027338:CDS |
| TTGGTACCTGCAAAACAACA+CGG | 0.687144 | 7.4:+49887491 | MS.gene027338:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAACTCATATATGAAAGAT+GGG | + | chr7.4:49887355-49887374 | MS.gene027338:CDS | 25.0% |
| ! | TCTTTCTAATTTGAAAGACT+TGG | + | chr7.4:49887530-49887549 | MS.gene027338:CDS | 25.0% |
| ! | TGTTTCAAAATCAAAAGGAT+CGG | - | chr7.4:49887286-49887305 | None:intergenic | 25.0% |
| !! | GTTAATTGCAAAAAGTATGA+AGG | + | chr7.4:49887557-49887576 | MS.gene027338:CDS | 25.0% |
| ACCTCTGTTTCAAAATCAAA+AGG | - | chr7.4:49887291-49887310 | None:intergenic | 30.0% | |
| CAGAACTCATATATGAAAGA+TGG | + | chr7.4:49887354-49887373 | MS.gene027338:CDS | 30.0% | |
| CTTTAGTAATCTGTTGCAAA+AGG | - | chr7.4:49887407-49887426 | None:intergenic | 30.0% | |
| TGTTGAATCATTGGAGATAA+AGG | - | chr7.4:49887169-49887188 | None:intergenic | 30.0% | |
| TTAGAAAGAGTCATATCATC+TGG | - | chr7.4:49887519-49887538 | None:intergenic | 30.0% | |
| !! | GAAGAAAAGTATCATCAAGA+AGG | + | chr7.4:49887212-49887231 | MS.gene027338:CDS | 30.0% |
| !!! | TCCTTTTGATTTTGAAACAG+AGG | + | chr7.4:49887287-49887306 | MS.gene027338:CDS | 30.0% |
| AGAGAACTCACTATTCTTTG+TGG | + | chr7.4:49887237-49887256 | MS.gene027338:CDS | 35.0% | |
| AGCCTTTCTTGTTGAATCAT+TGG | - | chr7.4:49887178-49887197 | None:intergenic | 35.0% | |
| CTCCAATGATTCAACAAGAA+AGG | + | chr7.4:49887173-49887192 | MS.gene027338:CDS | 35.0% | |
| GCAACAGATTACTAAAGCTA+GGG | + | chr7.4:49887410-49887429 | MS.gene027338:CDS | 35.0% | |
| GTTGCAAAAGGAAACTTTCT+TGG | - | chr7.4:49887395-49887414 | None:intergenic | 35.0% | |
| TGCAACAGATTACTAAAGCT+AGG | + | chr7.4:49887409-49887428 | MS.gene027338:CDS | 35.0% | |
| ! | ACTTTTCTTCCTCTTCTTGT+AGG | - | chr7.4:49887202-49887221 | None:intergenic | 35.0% |
| ! | GAAGGAAATTGATGACAAGA+TGG | + | chr7.4:49887575-49887594 | MS.gene027338:CDS | 35.0% |
| !!! | TTGATTTTGAAACAGAGGTG+TGG | + | chr7.4:49887292-49887311 | MS.gene027338:CDS | 35.0% |
| AAATCTGGAGGAAGCAAAAC+AGG | + | chr7.4:49887317-49887336 | MS.gene027338:CDS | 40.0% | |
| AAGCAAAACAGGTGATTGAG+AGG | + | chr7.4:49887328-49887347 | MS.gene027338:CDS | 40.0% | |
| AGCTGAACATTCGTATGAGT+TGG | + | chr7.4:49887472-49887491 | MS.gene027338:CDS | 40.0% | |
| AGGATCGGAAATTATAGCAC+AGG | - | chr7.4:49887271-49887290 | None:intergenic | 40.0% | |
| TTGGTACCTGCAAAACAACA+CGG | + | chr7.4:49887491-49887510 | MS.gene027338:CDS | 40.0% | |
| ! | TGTTTTGCTTCCTCCAGATT+TGG | - | chr7.4:49887318-49887337 | None:intergenic | 40.0% |
| CTTGTCTTTGCTTTCTCAGC+TGG | - | chr7.4:49887437-49887456 | None:intergenic | 45.0% | |
| GAAAGGCTTCCTACAAGAAG+AGG | + | chr7.4:49887190-49887209 | MS.gene027338:CDS | 45.0% | |
| TCGGAAATTATAGCACAGGC+TGG | - | chr7.4:49887267-49887286 | None:intergenic | 45.0% | |
| ! | AACAGAGGTGTGGCCAAATC+TGG | + | chr7.4:49887302-49887321 | MS.gene027338:CDS | 50.0% |
| ! | AGAGGTGTGGCCAAATCTGG+AGG | + | chr7.4:49887305-49887324 | MS.gene027338:CDS | 55.0% |
| !!! | CTGGCACCGTGTTGTTTTGC+AGG | - | chr7.4:49887500-49887519 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 49887141 | 49887620 | 49887141 | ID=MS.gene027338 |
| chr7.4 | mRNA | 49887141 | 49887620 | 49887141 | ID=MS.gene027338.t1;Parent=MS.gene027338 |
| chr7.4 | exon | 49887141 | 49887620 | 49887141 | ID=MS.gene027338.t1.exon1;Parent=MS.gene027338.t1 |
| chr7.4 | CDS | 49887141 | 49887620 | 49887141 | ID=cds.MS.gene027338.t1;Parent=MS.gene027338.t1 |
| Gene Sequence |
| Protein sequence |
