AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027368

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027368.t1 MTR_7g052480 97.661 342 8 0 2 343 215 556 0.0 705
MS.gene027368.t1 MTR_7g084090 55.357 336 146 3 12 343 422 757 3.04e-124 374
MS.gene027368.t1 MTR_6g061270 55.060 336 147 3 12 343 359 694 3.80e-122 367
MS.gene027368.t1 MTR_6g061200 51.190 336 131 4 12 343 359 665 1.77e-107 328
MS.gene027368.t1 MTR_5g013420 45.918 294 145 8 53 343 807 1089 1.53e-69 235
MS.gene027368.t1 MTR_5g013420 45.578 294 146 8 53 343 807 1089 2.55e-68 231
MS.gene027368.t1 MTR_4g010830 43.537 294 152 9 53 343 698 980 1.12e-64 221
MS.gene027368.t1 MTR_7g084090 49.321 221 108 3 12 228 422 642 2.54e-64 216
MS.gene027368.t1 MTR_4g010550 39.932 293 146 8 54 343 199 464 3.51e-57 192
MS.gene027368.t1 MTR_7g088370 30.816 331 200 6 14 339 395 701 4.73e-48 172
MS.gene027368.t1 MTR_3g011440 32.692 364 208 11 6 339 304 660 2.49e-44 161
MS.gene027368.t1 MTR_8g042750 34.471 293 179 6 53 339 399 684 4.29e-44 161
MS.gene027368.t1 MTR_7g445710 33.663 303 155 10 53 343 275 543 2.92e-43 157
MS.gene027368.t1 MTR_8g070075 32.680 306 188 10 14 313 456 749 1.12e-42 157
MS.gene027368.t1 MTR_8g070070 33.443 305 187 9 14 313 463 756 4.76e-42 155
MS.gene027368.t1 MTR_1g035420 34.926 272 164 7 53 321 392 653 1.36e-41 154
MS.gene027368.t1 MTR_1g035400 32.372 312 193 9 14 319 271 570 1.19e-40 150
MS.gene027368.t1 MTR_8g071030 34.470 264 152 6 57 313 487 736 4.83e-40 150
MS.gene027368.t1 MTR_7g450730 32.069 290 145 7 56 343 266 505 5.08e-39 145
MS.gene027368.t1 MTR_0376s0030 31.190 311 190 10 14 313 264 561 1.06e-37 142
MS.gene027368.t1 MTR_8g078490 29.942 344 173 10 38 343 940 1253 1.94e-35 137
MS.gene027368.t1 MTR_1g048950 32.907 313 162 9 38 343 262 533 1.01e-34 134
MS.gene027368.t1 MTR_6g044690 28.615 325 184 11 12 313 264 563 4.72e-33 129
MS.gene027368.t1 MTR_7g445530 32.721 272 137 10 53 312 275 512 7.32e-33 128
MS.gene027368.t1 MTR_4g132610 36.652 221 115 5 124 343 851 1047 2.23e-31 125
MS.gene027368.t1 MTR_5g018850 30.723 332 158 11 53 343 1207 1507 3.61e-31 124
MS.gene027368.t1 MTR_1g069570 30.792 341 150 14 57 339 430 742 2.47e-30 122
MS.gene027368.t1 MTR_6g043960 28.155 309 192 12 19 313 189 481 2.32e-29 117
MS.gene027368.t1 MTR_1g080340 29.912 341 160 11 56 341 268 584 1.73e-28 116
MS.gene027368.t1 MTR_8g078505 35.616 219 105 5 125 338 346 533 3.46e-28 115
MS.gene027368.t1 MTR_7g098390 28.743 334 164 11 57 339 388 698 6.50e-26 108
MS.gene027368.t1 MTR_2g054460 29.858 211 119 4 115 313 270 463 2.36e-24 103
MS.gene027368.t1 MTR_7g445600 32.692 156 62 1 157 312 243 355 2.20e-22 96.7
MS.gene027368.t1 MTR_1g069570 29.779 272 109 12 57 271 430 676 3.09e-19 89.4
MS.gene027368.t1 MTR_1g012890 28.814 177 92 5 166 342 225 367 1.30e-12 68.6
MS.gene027368.t1 MTR_1g064770 47.368 57 29 1 140 195 31 87 8.21e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027368.t1 AT5G13960 55.357 336 145 4 12 343 156 490 1.06e-129 380
MS.gene027368.t1 AT5G13960 55.357 336 145 4 12 343 290 624 9.28e-129 382
MS.gene027368.t1 AT2G22740 38.558 319 158 8 42 343 493 790 1.85e-56 196
MS.gene027368.t1 AT2G22740 38.558 319 158 8 42 343 493 790 1.85e-56 196
MS.gene027368.t1 AT2G35160 38.062 289 140 8 57 343 507 758 2.87e-55 192
MS.gene027368.t1 AT2G35160 38.062 289 140 8 57 343 543 794 4.61e-55 192
MS.gene027368.t1 AT2G35160 38.062 289 140 8 57 343 543 794 4.61e-55 192
MS.gene027368.t1 AT2G35160 38.062 289 140 8 57 343 543 794 4.61e-55 192
MS.gene027368.t1 AT2G35160 38.062 289 140 8 57 343 543 794 4.61e-55 192
MS.gene027368.t1 AT1G73100 33.333 297 187 6 47 339 376 665 2.43e-47 170
MS.gene027368.t1 AT2G33290 35.577 312 167 11 32 321 346 645 2.83e-44 161
MS.gene027368.t1 AT2G33290 35.577 312 167 11 32 321 346 645 2.83e-44 161
MS.gene027368.t1 AT2G33290 35.577 312 167 11 32 321 346 645 2.83e-44 161
MS.gene027368.t1 AT2G33290 35.577 312 167 11 32 321 346 645 2.83e-44 161
MS.gene027368.t1 AT4G13460 32.581 310 193 9 14 317 342 641 1.17e-42 157
MS.gene027368.t1 AT4G13460 32.581 310 193 9 14 317 342 641 1.17e-42 157
MS.gene027368.t1 AT5G04940 32.667 300 184 7 47 339 378 666 3.71e-42 155
MS.gene027368.t1 AT5G04940 32.667 300 184 7 47 339 378 666 3.71e-42 155
MS.gene027368.t1 AT1G17770 31.579 304 187 9 47 339 396 689 2.77e-36 139
MS.gene027368.t1 AT2G23740 32.544 338 166 14 38 342 1066 1374 6.27e-33 130
MS.gene027368.t1 AT2G23740 32.544 338 166 14 38 342 1073 1381 6.52e-33 130
MS.gene027368.t1 AT2G23740 32.544 338 166 14 38 342 1073 1381 6.52e-33 130
MS.gene027368.t1 AT2G23740 32.544 338 166 14 38 342 1073 1381 6.52e-33 130
MS.gene027368.t1 AT3G04380 28.402 338 166 10 57 341 119 433 6.88e-27 110
MS.gene027368.t1 AT3G04380 28.402 338 166 10 57 341 103 417 7.03e-27 110
MS.gene027368.t1 AT3G04380 28.402 338 166 10 57 341 119 433 7.27e-27 110
MS.gene027368.t1 AT3G04380 28.402 338 166 10 57 341 146 460 9.39e-27 110
MS.gene027368.t1 AT3G04380 28.402 338 166 10 57 341 130 444 9.57e-27 110
MS.gene027368.t1 AT3G04380 28.402 338 166 10 57 341 146 460 1.07e-26 110
MS.gene027368.t1 AT2G24740 30.034 293 181 10 53 339 477 751 3.66e-26 110
MS.gene027368.t1 AT1G04050 32.524 206 109 6 140 341 438 617 1.28e-24 105
MS.gene027368.t1 AT1G04050 32.524 206 109 6 140 341 542 721 1.29e-24 105
MS.gene027368.t1 AT1G04050 32.524 206 109 6 140 341 542 721 1.29e-24 105
MS.gene027368.t1 AT3G03750 31.933 238 126 7 102 338 143 345 8.64e-23 97.8
MS.gene027368.t1 AT2G23740 32.959 267 121 13 38 271 1073 1314 1.59e-22 99.4
MS.gene027368.t1 AT2G23740 32.959 267 121 13 38 271 1073 1314 1.59e-22 99.4
MS.gene027368.t1 AT4G30860 28.218 202 103 6 141 342 305 464 2.08e-12 68.2

Find 74 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCATAGATGCTGGTTCTTT+TGG 0.238650 7.4:+50314165 MS.gene027368:CDS
GAAACTTTGTTTCCACATTT+AGG 0.257839 7.4:-50313666 None:intergenic
TTGGCTTTAGGTTCATTTAA+TGG 0.273794 7.4:+50312861 MS.gene027368:intron
TCTTACCGCCCAACCTTTCT+TGG 0.287241 7.4:-50313820 None:intergenic
CCAGCATCTATGCAATATTC+AGG 0.354345 7.4:-50314156 None:intergenic
CACACAATCAATAGTGTAGA+TGG 0.372069 7.4:+50313960 MS.gene027368:CDS
AGAAGACATACCTCCCTATC+AGG 0.387240 7.4:+50314296 MS.gene027368:CDS
TGTTCTTGTGCTAATCATAA+TGG 0.392666 7.4:+50313395 MS.gene027368:CDS
GCACCAGTTGTCGAGTATAT+TGG 0.400887 7.4:+50313867 MS.gene027368:CDS
ATTGATAACGAATACGTTCC+TGG 0.401461 7.4:+50312321 MS.gene027368:CDS
TGGAAACAAAGTTTCTCAAC+AGG 0.404546 7.4:+50313674 MS.gene027368:CDS
AGTATCTGATAAATAAGCTT+CGG 0.405640 7.4:+50312358 MS.gene027368:CDS
TTGCATCCCGCAGCACTAGA+TGG 0.412772 7.4:-50313344 None:intergenic
GTATCTGATAAATAAGCTTC+GGG 0.422102 7.4:+50312359 MS.gene027368:CDS
CTTACCGCCCAACCTTTCTT+GGG 0.422197 7.4:-50313819 None:intergenic
GATAAATTGTTTATAGGCAA+TGG 0.426330 7.4:-50314102 None:intergenic
CAGGAACTCACATATGACTA+TGG 0.430156 7.4:+50314494 MS.gene027368:intron
ATTAGCACAAGAACATCGTT+TGG 0.435566 7.4:-50313387 None:intergenic
ACACATTGTACAAAGAGGTT+TGG 0.458185 7.4:-50314222 None:intergenic
ATCGTACTCCCAAGAAAGGT+TGG 0.459933 7.4:+50313811 MS.gene027368:CDS
TACAAAGAGGAATACCTGAT+AGG 0.463396 7.4:-50314310 None:intergenic
TGGAGGATCATCAAAATCAT+TGG 0.466153 7.4:-50313074 None:intergenic
ACACCAATATACTCGACAAC+TGG 0.483092 7.4:-50313870 None:intergenic
ACATGGGACTTTATCCCATC+AGG 0.487648 7.4:+50313843 MS.gene027368:CDS
ACAAAGAGGAATACCTGATA+GGG 0.490411 7.4:-50314309 None:intergenic
GCTTCGGGAACTTGCTAAAG+TGG 0.493084 7.4:+50312374 MS.gene027368:CDS
TCGTACTCCCAAGAAAGGTT+GGG 0.500282 7.4:+50313812 MS.gene027368:CDS
CTAGATGGAACTTCAATGCT+TGG 0.505910 7.4:-50313329 None:intergenic
TACACACTCGATAGTGTGTC+TGG 0.507324 7.4:+50314518 MS.gene027368:CDS
CGACAACTGGTGCACCTGAT+GGG 0.509774 7.4:-50313857 None:intergenic
TACTTCGAAGTAGTGATTCC+AGG 0.513375 7.4:-50312339 None:intergenic
GTTTATCGTACTCCCAAGAA+AGG 0.517387 7.4:+50313807 MS.gene027368:CDS
TCGACAACTGGTGCACCTGA+TGG 0.518260 7.4:-50313858 None:intergenic
GTTTCAAGATTTATCAACCA+TGG 0.524532 7.4:+50314192 MS.gene027368:CDS
GAAGTTCCATCTAGTGCTGC+GGG 0.529357 7.4:+50313338 MS.gene027368:CDS
AAACTCTTTCTTCTTCTCCA+AGG 0.531848 7.4:+50312899 MS.gene027368:CDS
TGCTAAAGTGGGAACATACA+AGG 0.533120 7.4:+50312386 MS.gene027368:CDS
TTTCATACAATAACATTGGC+CGG 0.533464 7.4:+50313426 MS.gene027368:CDS
CCTGAATATTGCATAGATGC+TGG 0.535805 7.4:+50314156 MS.gene027368:CDS
CTAAGTAGAGACGATGAGCT+AGG 0.538306 7.4:+50313894 MS.gene027368:CDS
TTTAGGTTCATTTAATGGAA+GGG 0.538367 7.4:+50312866 MS.gene027368:intron
CTTTAGGTTCATTTAATGGA+AGG 0.538474 7.4:+50312865 MS.gene027368:intron
GATAGTGTGTCTGGTTCTGA+TGG 0.539465 7.4:+50314527 MS.gene027368:CDS
AGAGGTTTGGCTCACATCCA+TGG 0.542639 7.4:-50314209 None:intergenic
CTAGACATCGTGGTCGAATG+TGG 0.546755 7.4:+50313624 MS.gene027368:CDS
ACAATGTCATTGTGGTTCAA+AGG 0.554813 7.4:+50314565 MS.gene027368:CDS
AAGGTTGGGCGGTAAGAACA+TGG 0.558115 7.4:+50313826 MS.gene027368:CDS
GGGCTTAAGTTACCGACTTG+AGG 0.574958 7.4:+50313695 MS.gene027368:CDS
TGAGAACACATTGTACAAAG+AGG 0.579762 7.4:-50314227 None:intergenic
GAAGATATAATCATTTCCGT+TGG 0.584617 7.4:-50313922 None:intergenic
TGTGGACCGCAATGTGGATG+CGG 0.588020 7.4:+50313642 MS.gene027368:CDS
TGCTGCGGGATGCAATTGCA+AGG 0.591722 7.4:+50313352 MS.gene027368:CDS
GTATGCAAGGACATTAGCAA+TGG 0.598177 7.4:+50313025 MS.gene027368:CDS
TTACCTGTGGGTGCAACTGG+AGG 0.602916 7.4:-50313091 None:intergenic
CTTCGGGAACTTGCTAAAGT+GGG 0.602929 7.4:+50312375 MS.gene027368:CDS
GATCCTCCAGTTGCACCCAC+AGG 0.606455 7.4:+50313088 MS.gene027368:CDS
TGTGGATGCGGTCCTAAATG+TGG 0.617446 7.4:+50313654 MS.gene027368:CDS
AGCACTACACGAGCTAGTCT+AGG 0.619841 7.4:-50314264 None:intergenic
AATGTTATTGTATGAAAACT+CGG 0.621218 7.4:-50313420 None:intergenic
CATTCGACCACGATGTCTAG+AGG 0.624366 7.4:-50313621 None:intergenic
CAATAGTGTAGATGGAAGAG+AGG 0.628379 7.4:+50313968 MS.gene027368:CDS
ATTGTAAAATGACCTCAAGT+CGG 0.632516 7.4:-50313707 None:intergenic
TGAAGTTCCATCTAGTGCTG+CGG 0.634941 7.4:+50313337 MS.gene027368:CDS
AAACAGCTACAATGTCATTG+TGG 0.648614 7.4:+50314557 MS.gene027368:CDS
AAGAGGAATACCTGATAGGG+AGG 0.649275 7.4:-50314306 None:intergenic
GCTGCGGGATGCAATTGCAA+GGG 0.655088 7.4:+50313353 MS.gene027368:CDS
AGGTTGGGCGGTAAGAACAT+GGG 0.665196 7.4:+50313827 MS.gene027368:CDS
TACTCCCAAGAAAGGTTGGG+CGG 0.669811 7.4:+50313815 MS.gene027368:CDS
GTCGAATGTGGACCGCAATG+TGG 0.680163 7.4:+50313636 MS.gene027368:CDS
GGAAACAAAGTTTCTCAACA+GGG 0.682009 7.4:+50313675 MS.gene027368:CDS
TTAGGACCGCATCCACATTG+CGG 0.698881 7.4:-50313648 None:intergenic
GATGAGCTAGGAAGTGCCAA+CGG 0.706121 7.4:+50313906 MS.gene027368:CDS
GATTGAGCCTCTAGACATCG+TGG 0.722557 7.4:+50313614 MS.gene027368:CDS
GAGCTAGTCTAGGATCTCTG+TGG 0.733830 7.4:-50314254 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATATGTATGAAATTT+AGG + chr7.4:50314022-50314041 MS.gene027368:intron 10.0%
!! GTAAAAATTTAATAAACAAT+TGG + chr7.4:50314454-50314473 MS.gene027368:intron 10.0%
!! TAAAATATGTATGAAATTTA+GGG + chr7.4:50314023-50314042 MS.gene027368:intron 10.0%
!!! GTTTTTTCATTTTTAAAAAA+GGG - chr7.4:50313143-50313162 None:intergenic 10.0%
!!! TAATATCTTATTAGAATTTT+GGG + chr7.4:50312670-50312689 MS.gene027368:intron 10.0%
!!! TGTTTTTTCATTTTTAAAAA+AGG - chr7.4:50313144-50313163 None:intergenic 10.0%
!!! TTAATATCTTATTAGAATTT+TGG + chr7.4:50312669-50312688 MS.gene027368:intron 10.0%
!!! TTTTTTTTTTTTAAACATGA+TGG + chr7.4:50314387-50314406 MS.gene027368:intron 10.0%
!! AAACAATTTATCTGAAAAAA+GGG + chr7.4:50314113-50314132 MS.gene027368:CDS 15.0%
!! GAAAAAAAAAAAAGGAAAAA+GGG - chr7.4:50313187-50313206 None:intergenic 15.0%
!! GTAAAACAAAAAATATTAGA+TGG + chr7.4:50313110-50313129 MS.gene027368:intron 15.0%
!! TAAACAATTTATCTGAAAAA+AGG + chr7.4:50314112-50314131 MS.gene027368:CDS 15.0%
!!! TCATGATGTTTTAAAATTTT+AGG + chr7.4:50313275-50313294 MS.gene027368:intron 15.0%
!!! TTATTAAATTTTTACCATGA+AGG - chr7.4:50314449-50314468 None:intergenic 15.0%
!!! TTTTCAGATAAATTGTTTAT+AGG - chr7.4:50314111-50314130 None:intergenic 15.0%
!! AATGTTATTGTATGAAAACT+CGG - chr7.4:50313423-50313442 None:intergenic 20.0%
!! CGAAAAAAAAAAAAGGAAAA+AGG - chr7.4:50313188-50313207 None:intergenic 20.0%
!! TAAAGTTACTCCTAATAAAT+TGG - chr7.4:50312166-50312185 None:intergenic 20.0%
!! TCGTATTCTAATATTCAATT+TGG + chr7.4:50312842-50312861 MS.gene027368:intron 20.0%
!! TTACATATTACAAACATCAA+TGG - chr7.4:50313758-50313777 None:intergenic 20.0%
!! TTCTAATAAGATATTAAAGC+CGG - chr7.4:50312666-50312685 None:intergenic 20.0%
!!! AAAATTGAACAAAAGTACAA+AGG - chr7.4:50312519-50312538 None:intergenic 20.0%
!!! AATATTTTTTGTTTTACCTG+TGG - chr7.4:50313107-50313126 None:intergenic 20.0%
!!! ATATTTTTTGTTTTACCTGT+GGG - chr7.4:50313106-50313125 None:intergenic 20.0%
!!! ATTTGTTTTTTTTCTTGTCA+GGG + chr7.4:50313466-50313485 MS.gene027368:intron 20.0%
!!! GAATATTGTTTCTTTTAGTT+TGG + chr7.4:50313003-50313022 MS.gene027368:intron 20.0%
! AATATGTATGAAATTTAGGG+CGG + chr7.4:50314026-50314045 MS.gene027368:intron 25.0%
! AGTATCTGATAAATAAGCTT+CGG + chr7.4:50312358-50312377 MS.gene027368:CDS 25.0%
! ATCCAAAAGTAAAACAATAC+CGG - chr7.4:50313448-50313467 None:intergenic 25.0%
! ATTAGAATACGACATATAAG+TGG - chr7.4:50312834-50312853 None:intergenic 25.0%
! GATAAATTGTTTATAGGCAA+TGG - chr7.4:50314105-50314124 None:intergenic 25.0%
!! ATTAACTTTCATTTTGTTGC+AGG + chr7.4:50312284-50312303 MS.gene027368:intron 25.0%
!! GAGTTTTCATACAATAACAT+TGG + chr7.4:50313422-50313441 MS.gene027368:CDS 25.0%
!! GGTATATTTTCTAACTTAAC+AGG + chr7.4:50314475-50314494 MS.gene027368:intron 25.0%
!! TTTAGGTTCATTTAATGGAA+GGG + chr7.4:50312866-50312885 MS.gene027368:intron 25.0%
!! TTTTATGATAACTTCCTTCA+TGG + chr7.4:50314432-50314451 MS.gene027368:intron 25.0%
!!! ATCTTATTAGAATTTTGGGT+TGG + chr7.4:50312674-50312693 MS.gene027368:intron 25.0%
!!! CTAATATTCAATTTGGCTTT+AGG + chr7.4:50312849-50312868 MS.gene027368:intron 25.0%
!!! GATTTGTTTTTTTTCTTGTC+AGG + chr7.4:50313465-50313484 MS.gene027368:intron 25.0%
!!! GGTTTGTTATTGCATATATA+TGG + chr7.4:50314408-50314427 MS.gene027368:intron 25.0%
!!! TCTTTGAAATTTTTCAGAGA+CGG + chr7.4:50314067-50314086 MS.gene027368:intron 25.0%
AAGGAAAAAGGGACATATAT+TGG - chr7.4:50313176-50313195 None:intergenic 30.0%
AGGTATTCCTCTTTGTAATA+AGG + chr7.4:50314316-50314335 MS.gene027368:intron 30.0%
ATTGTAAAATGACCTCAAGT+CGG - chr7.4:50313710-50313729 None:intergenic 30.0%
CATGCAGCGAAAAAAAAAAA+AGG - chr7.4:50313195-50313214 None:intergenic 30.0%
CTAAGATCCTTATTACAAAG+AGG - chr7.4:50314326-50314345 None:intergenic 30.0%
CTCTTTGTAATAAGGATCTT+AGG + chr7.4:50314324-50314343 MS.gene027368:intron 30.0%
CTTACAAAATCAGCTTGTAA+AGG + chr7.4:50312709-50312728 MS.gene027368:intron 30.0%
GAAGATATAATCATTTCCGT+TGG - chr7.4:50313925-50313944 None:intergenic 30.0%
GTATCTGATAAATAAGCTTC+GGG + chr7.4:50312359-50312378 MS.gene027368:CDS 30.0%
GTTTCAAGATTTATCAACCA+TGG + chr7.4:50314192-50314211 MS.gene027368:CDS 30.0%
TGTTCTTGTGCTAATCATAA+TGG + chr7.4:50313395-50313414 MS.gene027368:CDS 30.0%
TTTCATACAATAACATTGGC+CGG + chr7.4:50313426-50313445 MS.gene027368:CDS 30.0%
TTTCTTGCTGCCAATTTATT+AGG + chr7.4:50312153-50312172 MS.gene027368:intron 30.0%
! AAGAGATAAAGCCTTATCAT+GGG - chr7.4:50312758-50312777 None:intergenic 30.0%
! CTTTAGGTTCATTTAATGGA+AGG + chr7.4:50312865-50312884 MS.gene027368:intron 30.0%
! GAAACTTTGTTTCCACATTT+AGG - chr7.4:50313669-50313688 None:intergenic 30.0%
!! CTTTACAAGCTGATTTTGTA+AGG - chr7.4:50312711-50312730 None:intergenic 30.0%
!! GATTTGTTGATAACATACCT+TGG - chr7.4:50312919-50312938 None:intergenic 30.0%
!! TTGGCTTTAGGTTCATTTAA+TGG + chr7.4:50312861-50312880 MS.gene027368:intron 30.0%
!!! TTCTTTTAGTTTGGTATGCA+AGG + chr7.4:50313012-50313031 MS.gene027368:intron 30.0%
AAACAGCTACAATGTCATTG+TGG + chr7.4:50314557-50314576 MS.gene027368:CDS 35.0%
AAACTACCTTTCTCTTACAC+AGG + chr7.4:50313785-50313804 MS.gene027368:intron 35.0%
AAACTCTTTCTTCTTCTCCA+AGG + chr7.4:50312899-50312918 MS.gene027368:CDS 35.0%
AAACTTCAAATGTCCAGTCA+TGG - chr7.4:50312593-50312612 None:intergenic 35.0%
ACAAAGAGGAATACCTGATA+GGG - chr7.4:50314312-50314331 None:intergenic 35.0%
ACAATGTCATTGTGGTTCAA+AGG + chr7.4:50314565-50314584 MS.gene027368:CDS 35.0%
ACACATTGTACAAAGAGGTT+TGG - chr7.4:50314225-50314244 None:intergenic 35.0%
ATTAGCACAAGAACATCGTT+TGG - chr7.4:50313390-50313409 None:intergenic 35.0%
CACACAATCAATAGTGTAGA+TGG + chr7.4:50313960-50313979 MS.gene027368:CDS 35.0%
GATAAACCTGTGTAAGAGAA+AGG - chr7.4:50313794-50313813 None:intergenic 35.0%
GGAAACAAAGTTTCTCAACA+GGG + chr7.4:50313675-50313694 MS.gene027368:CDS 35.0%
TACAAAGAGGAATACCTGAT+AGG - chr7.4:50314313-50314332 None:intergenic 35.0%
TACTACCAGATTTCCACTAT+CGG - chr7.4:50312619-50312638 None:intergenic 35.0%
TATCATGGGTTTATAGGTGA+GGG - chr7.4:50312744-50312763 None:intergenic 35.0%
TCACCTATAAACCCATGATA+AGG + chr7.4:50312744-50312763 MS.gene027368:intron 35.0%
TGAAAAAAGGGACGAGTTAA+TGG + chr7.4:50314125-50314144 MS.gene027368:CDS 35.0%
TGAGAACACATTGTACAAAG+AGG - chr7.4:50314230-50314249 None:intergenic 35.0%
TGGAAACAAAGTTTCTCAAC+AGG + chr7.4:50313674-50313693 MS.gene027368:CDS 35.0%
TGGAGGATCATCAAAATCAT+TGG - chr7.4:50313077-50313096 None:intergenic 35.0%
TTATCATGGGTTTATAGGTG+AGG - chr7.4:50312745-50312764 None:intergenic 35.0%
TTGTTGCAGGTAGAGAAAAA+TGG + chr7.4:50312297-50312316 MS.gene027368:intron 35.0%
! AAGCCTTATCATGGGTTTAT+AGG - chr7.4:50312750-50312769 None:intergenic 35.0%
! GAAGAGATAAAGCCTTATCA+TGG - chr7.4:50312759-50312778 None:intergenic 35.0%
!! ATTGATAACGAATACGTTCC+TGG + chr7.4:50312321-50312340 MS.gene027368:CDS 35.0%
!! CAAGCTGATTTTGTAAGGTT+TGG - chr7.4:50312706-50312725 None:intergenic 35.0%
!! GAAATTTTTCAGAGACGGTT+GGG + chr7.4:50314072-50314091 MS.gene027368:intron 35.0%
!! TGAAATTTTTCAGAGACGGT+TGG + chr7.4:50314071-50314090 MS.gene027368:intron 35.0%
!! TTTTCGCTGCATGCTTTATT+TGG + chr7.4:50313202-50313221 MS.gene027368:intron 35.0%
AAAATCAGCTTGTAAAGGCG+AGG + chr7.4:50312714-50312733 MS.gene027368:intron 40.0%
ACACCAATATACTCGACAAC+TGG - chr7.4:50313873-50313892 None:intergenic 40.0%
ACTACCAGATTTCCACTATC+GGG - chr7.4:50312618-50312637 None:intergenic 40.0%
AGTGAGCCGATAGTGAATAT+TGG + chr7.4:50312639-50312658 MS.gene027368:intron 40.0%
CAATAGTGTAGATGGAAGAG+AGG + chr7.4:50313968-50313987 MS.gene027368:CDS 40.0%
CAGGAACTCACATATGACTA+TGG + chr7.4:50314494-50314513 MS.gene027368:intron 40.0%
CCAGCATCTATGCAATATTC+AGG - chr7.4:50314159-50314178 None:intergenic 40.0%
CCTGAATATTGCATAGATGC+TGG + chr7.4:50314156-50314175 MS.gene027368:CDS 40.0%
CTAGATGGAACTTCAATGCT+TGG - chr7.4:50313332-50313351 None:intergenic 40.0%
GTATGCAAGGACATTAGCAA+TGG + chr7.4:50313025-50313044 MS.gene027368:CDS 40.0%
GTTTATCGTACTCCCAAGAA+AGG + chr7.4:50313807-50313826 MS.gene027368:CDS 40.0%
TACTTCGAAGTAGTGATTCC+AGG - chr7.4:50312342-50312361 None:intergenic 40.0%
TGCTAAAGTGGGAACATACA+AGG + chr7.4:50312386-50312405 MS.gene027368:CDS 40.0%
! TTTGAAGTTTGACCCGATAG+TGG + chr7.4:50312603-50312622 MS.gene027368:intron 40.0%
!! GGCCGGTATTGTTTTACTTT+TGG + chr7.4:50313443-50313462 MS.gene027368:intron 40.0%
!!! TGCATAGATGCTGGTTCTTT+TGG + chr7.4:50314165-50314184 MS.gene027368:CDS 40.0%
AAGAGGAATACCTGATAGGG+AGG - chr7.4:50314309-50314328 None:intergenic 45.0%
ACATGGGACTTTATCCCATC+AGG + chr7.4:50313843-50313862 MS.gene027368:CDS 45.0%
AGAAGACATACCTCCCTATC+AGG + chr7.4:50314296-50314315 MS.gene027368:CDS 45.0%
ATCGTACTCCCAAGAAAGGT+TGG + chr7.4:50313811-50313830 MS.gene027368:CDS 45.0%
CGAGTTGTATCATGCAGCTA+GGG - chr7.4:50312206-50312225 None:intergenic 45.0%
CTAAGTAGAGACGATGAGCT+AGG + chr7.4:50313894-50313913 MS.gene027368:CDS 45.0%
GCACCAGTTGTCGAGTATAT+TGG + chr7.4:50313867-50313886 MS.gene027368:CDS 45.0%
GCCGATAGTGAATATTGGAC+CGG + chr7.4:50312644-50312663 MS.gene027368:intron 45.0%
GCCGGTCCAATATTCACTAT+CGG - chr7.4:50312648-50312667 None:intergenic 45.0%
TACACACTCGATAGTGTGTC+TGG + chr7.4:50314518-50314537 MS.gene027368:CDS 45.0%
TCGTACTCCCAAGAAAGGTT+GGG + chr7.4:50313812-50313831 MS.gene027368:CDS 45.0%
TGAAGTTCCATCTAGTGCTG+CGG + chr7.4:50313337-50313356 MS.gene027368:CDS 45.0%
!! CTTCGGGAACTTGCTAAAGT+GGG + chr7.4:50312375-50312394 MS.gene027368:CDS 45.0%
!! GATAGTGTGTCTGGTTCTGA+TGG + chr7.4:50314527-50314546 MS.gene027368:CDS 45.0%
!! TTGACCCGATAGTGGAAATC+TGG + chr7.4:50312611-50312630 MS.gene027368:intron 45.0%
AAGGTTGGGCGGTAAGAACA+TGG + chr7.4:50313826-50313845 MS.gene027368:CDS 50.0%
AGGTTGGGCGGTAAGAACAT+GGG + chr7.4:50313827-50313846 MS.gene027368:CDS 50.0%
CATTCGACCACGATGTCTAG+AGG - chr7.4:50313624-50313643 None:intergenic 50.0%
CTAGACATCGTGGTCGAATG+TGG + chr7.4:50313624-50313643 MS.gene027368:CDS 50.0%
CTTACCGCCCAACCTTTCTT+GGG - chr7.4:50313822-50313841 None:intergenic 50.0%
GAAGTTCCATCTAGTGCTGC+GGG + chr7.4:50313338-50313357 MS.gene027368:CDS 50.0%
GATTGAGCCTCTAGACATCG+TGG + chr7.4:50313614-50313633 MS.gene027368:CDS 50.0%
GCGAGTTGTATCATGCAGCT+AGG - chr7.4:50312207-50312226 None:intergenic 50.0%
TACTCCCAAGAAAGGTTGGG+CGG + chr7.4:50313815-50313834 MS.gene027368:CDS 50.0%
TCTTACCGCCCAACCTTTCT+TGG - chr7.4:50313823-50313842 None:intergenic 50.0%
TGTGGATGCGGTCCTAAATG+TGG + chr7.4:50313654-50313673 MS.gene027368:CDS 50.0%
TTAGGACCGCATCCACATTG+CGG - chr7.4:50313651-50313670 None:intergenic 50.0%
! AGAGGTTTGGCTCACATCCA+TGG - chr7.4:50314212-50314231 None:intergenic 50.0%
! AGCACTACACGAGCTAGTCT+AGG - chr7.4:50314267-50314286 None:intergenic 50.0%
! GAGCTAGTCTAGGATCTCTG+TGG - chr7.4:50314257-50314276 None:intergenic 50.0%
! GTTTTACCTGTGGGTGCAAC+TGG - chr7.4:50313097-50313116 None:intergenic 50.0%
!! GATGAGCTAGGAAGTGCCAA+CGG + chr7.4:50313906-50313925 MS.gene027368:CDS 50.0%
!! GCTTCGGGAACTTGCTAAAG+TGG + chr7.4:50312374-50312393 MS.gene027368:CDS 50.0%
!! GGGCTTAAGTTACCGACTTG+AGG + chr7.4:50313695-50313714 MS.gene027368:CDS 50.0%
CACTCTCTCACGTCCATGAC+TGG + chr7.4:50312577-50312596 MS.gene027368:intron 55.0%
CGACAACTGGTGCACCTGAT+GGG - chr7.4:50313860-50313879 None:intergenic 55.0%
GCTGCGGGATGCAATTGCAA+GGG + chr7.4:50313353-50313372 MS.gene027368:CDS 55.0%
GTCGAATGTGGACCGCAATG+TGG + chr7.4:50313636-50313655 MS.gene027368:CDS 55.0%
TCGACAACTGGTGCACCTGA+TGG - chr7.4:50313861-50313880 None:intergenic 55.0%
TGCTGCGGGATGCAATTGCA+AGG + chr7.4:50313352-50313371 MS.gene027368:CDS 55.0%
TGTGGACCGCAATGTGGATG+CGG + chr7.4:50313642-50313661 MS.gene027368:CDS 55.0%
TTACCTGTGGGTGCAACTGG+AGG - chr7.4:50313094-50313113 None:intergenic 55.0%
TTGCATCCCGCAGCACTAGA+TGG - chr7.4:50313347-50313366 None:intergenic 55.0%
GATCCTCCAGTTGCACCCAC+AGG + chr7.4:50313088-50313107 MS.gene027368:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 50312112 50314610 50312112 ID=MS.gene027368
chr7.4 mRNA 50312112 50314610 50312112 ID=MS.gene027368.t1;Parent=MS.gene027368
chr7.4 exon 50312112 50312114 50312112 ID=MS.gene027368.t1.exon1;Parent=MS.gene027368.t1
chr7.4 CDS 50312112 50312114 50312112 ID=cds.MS.gene027368.t1;Parent=MS.gene027368.t1
chr7.4 exon 50312306 50312407 50312306 ID=MS.gene027368.t1.exon2;Parent=MS.gene027368.t1
chr7.4 CDS 50312306 50312407 50312306 ID=cds.MS.gene027368.t1;Parent=MS.gene027368.t1
chr7.4 exon 50312871 50312920 50312871 ID=MS.gene027368.t1.exon3;Parent=MS.gene027368.t1
chr7.4 CDS 50312871 50312920 50312871 ID=cds.MS.gene027368.t1;Parent=MS.gene027368.t1
chr7.4 exon 50313021 50313109 50313021 ID=MS.gene027368.t1.exon4;Parent=MS.gene027368.t1
chr7.4 CDS 50313021 50313109 50313021 ID=cds.MS.gene027368.t1;Parent=MS.gene027368.t1
chr7.4 exon 50313297 50313447 50313297 ID=MS.gene027368.t1.exon5;Parent=MS.gene027368.t1
chr7.4 CDS 50313297 50313447 50313297 ID=cds.MS.gene027368.t1;Parent=MS.gene027368.t1
chr7.4 exon 50313611 50313716 50313611 ID=MS.gene027368.t1.exon6;Parent=MS.gene027368.t1
chr7.4 CDS 50313611 50313716 50313611 ID=cds.MS.gene027368.t1;Parent=MS.gene027368.t1
chr7.4 exon 50313807 50313989 50313807 ID=MS.gene027368.t1.exon7;Parent=MS.gene027368.t1
chr7.4 CDS 50313807 50313989 50313807 ID=cds.MS.gene027368.t1;Parent=MS.gene027368.t1
chr7.4 exon 50314084 50314317 50314084 ID=MS.gene027368.t1.exon8;Parent=MS.gene027368.t1
chr7.4 CDS 50314084 50314317 50314084 ID=cds.MS.gene027368.t1;Parent=MS.gene027368.t1
chr7.4 exon 50314497 50314610 50314497 ID=MS.gene027368.t1.exon9;Parent=MS.gene027368.t1
chr7.4 CDS 50314497 50314610 50314497 ID=cds.MS.gene027368.t1;Parent=MS.gene027368.t1
Gene Sequence

>MS.gene027368

ATGGTATGTTTGATGAGCATGTAATTGAATCAATTAGTCAATTTCTTGCTGCCAATTTATTAGGAGTAACTTTAGTTTTATGCTTCAGCTGCCCTAGCTGCATGATACAACTCGCAGCATATATTGATGAAAATAGTCTAATGATGTATATGATAATAATTTTTTTTATTCAATTAACTTTCATTTTGTTGCAGGTAGAGAAAAATGGCATTGATAACGAATACGTTCCTGGAATCACTACTTCGAAGTATCTGATAAATAAGCTTCGGGAACTTGCTAAAGTGGGAACATACAAGGTTGTATATATTACGTTTATTTGATTTCTTTTTCATATCTATGACTCAATGTCAATGATATTTCACTCTTTACTCTCATATATGTGAGATGATTAGTTTATATAGCATCCTTTGTACTTTTGTTCAATTTTTCTCTATAGTTTATATTCTCAATGTGAGATTTTTAACACACTCTCTCACGTCCATGACTGGACATTTGAAGTTTGACCCGATAGTGGAAATCTGGTAGTAAGTGAGCCGATAGTGAATATTGGACCGGCTTTAATATCTTATTAGAATTTTGGGTTGGATCTAACCAAACCTTACAAAATCAGCTTGTAAAGGCGAGGATTTCCCTCACCTATAAACCCATGATAAGGCTTTATCTCTTCTAATCAAGACTCTTAACATTATGTTTACATTTGATATATATATCATTTTGCTCCACTTATATGTCGTATTCTAATATTCAATTTGGCTTTAGGTTCATTTAATGGAAGGGCAAGTCTCTAAAACTCTTTCTTCTTCTCCAAGGTATGTTATCAACAAATCTCTTCAGAAATACAGTGTTATTGAGTTCTTTTGATATACATAAATATCTGTAATTGAGTTACAAGAATATTGTTTCTTTTAGTTTGGTATGCAAGGACATTAGCAATGGACAAGAAGCTATCAGCATTATTGCAACCAATGATTTTGATGATCCTCCAGTTGCACCCACAGGTAAAACAAAAAATATTAGATGGAAATGAACCCTTTTTTAAAAATGAAAAAACAAATGCAAATCCAATATATGTCCCTTTTTCCTTTTTTTTTTTTCGCTGCATGCTTTATTTGGTGAAGTGCATATGTTTTCAAGTCACTCTCTTTTGATACAGAAAATTGCACTCATGATGTTTTAAAATTTTAGGTTTTGAATACATTACATCAAACAAATTTTCACCAAGCATTGAAGTTCCATCTAGTGCTGCGGGATGCAATTGCAAGGGAAGTTGTAGAACCAAACGATGTTCTTGTGCTAATCATAATGGCTCCGAGTTTTCATACAATAACATTGGCCGGTATTGTTTTACTTTTGGATTTGTTTTTTTTCTTGTCAGGGTTAACACAAAGTTAGATCTTACGTTGTAAATAAATATAATTTCACTTGTTTTAAATAGTTTGATGATCAATACTCTTAAAATTAAGTTCTTTACTAACAAACTTAATCAATTATTTTGTTAGATTGATTGAGCCTCTAGACATCGTGGTCGAATGTGGACCGCAATGTGGATGCGGTCCTAAATGTGGAAACAAAGTTTCTCAACAGGGCTTAAGTTACCGACTTGAGGTCATTTTACAATTTACTCAAGTTTCTACACTTGTTTACCATTGATGTTTGTAATATGTAAACTAACTAAACTACCTTTCTCTTACACAGGTTTATCGTACTCCCAAGAAAGGTTGGGCGGTAAGAACATGGGACTTTATCCCATCAGGTGCACCAGTTGTCGAGTATATTGGTGTACTAAGTAGAGACGATGAGCTAGGAAGTGCCAACGGAAATGATTATATCTTCGATATTGATTGTTTGCACACAATCAATAGTGTAGATGGAAGAGAGGTAAGAATGCAATTTTTTTTTGCAAAATTATTATAAAATATGTATGAAATTTAGGGCGGTATTATTATAGATTTCTTTCTTTGAAATTTTTCAGAGACGGTTGGGTAATGTACCATTGCCTATAAACAATTTATCTGAAAAAAGGGACGAGTTAATGGAAAAAGATCCTGAATATTGCATAGATGCTGGTTCTTTTGGTAATGTTTCAAGATTTATCAACCATGGATGTGAGCCAAACCTCTTTGTACAATGTGTTCTCAGCAGCCACAGAGATCCTAGACTAGCTCGTGTAGTGCTATTTGCAGCAGAAGACATACCTCCCTATCAGGTATTCCTCTTTGTAATAAGGATCTTAGGAATTTCTTGTGTTATCAACTTTTTTATTTGATTCACAATGTTTTTTTTTTTTAAACATGATGGTTTGTTATTGCATATATATGGATTTTATGATAACTTCCTTCATGGTAAAAATTTAATAAACAATTGGTATATTTTCTAACTTAACAGGAACTCACATATGACTATGGTTACACACTCGATAGTGTGTCTGGTTCTGATGGAAAAATCAAACAGCTACAATGTCATTGTGGTTCAAAGGAATGTCGTAAGCGCTTGTACTAG
Protein sequence

>MS.gene027368.t1

MVEKNGIDNEYVPGITTSKYLINKLRELAKVGTYKVHLMEGQVSKTLSSSPSLVCKDISNGQEAISIIATNDFDDPPVAPTGFEYITSNKFSPSIEVPSSAAGCNCKGSCRTKRCSCANHNGSEFSYNNIGRLIEPLDIVVECGPQCGCGPKCGNKVSQQGLSYRLEVYRTPKKGWAVRTWDFIPSGAPVVEYIGVLSRDDELGSANGNDYIFDIDCLHTINSVDGRERRLGNVPLPINNLSEKRDELMEKDPEYCIDAGSFGNVSRFINHGCEPNLFVQCVLSSHRDPRLARVVLFAAEDIPPYQELTYDYGYTLDSVSGSDGKIKQLQCHCGSKECRKRLY