Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027372.t1 | AES79015.2 | 97.9 | 96 | 2 | 0 | 1 | 96 | 461 | 556 | 2.60E-50 | 207.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027372.t1 | Q8GZB6 | 76.6 | 94 | 22 | 0 | 3 | 96 | 531 | 624 | 6.6e-39 | 161.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027372.t1 | G7KY38 | 97.9 | 96 | 2 | 0 | 1 | 96 | 461 | 556 | 1.8e-50 | 207.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene027372.t1 | TR | SET |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027372.t1 | MTR_7g052480 | 97.917 | 96 | 2 | 0 | 1 | 96 | 461 | 556 | 8.75e-65 | 204 |
| MS.gene027372.t1 | MTR_6g061200 | 75.532 | 94 | 23 | 0 | 3 | 96 | 572 | 665 | 5.44e-46 | 156 |
| MS.gene027372.t1 | MTR_7g084090 | 75.532 | 94 | 23 | 0 | 3 | 96 | 664 | 757 | 8.18e-46 | 156 |
| MS.gene027372.t1 | MTR_6g061270 | 74.468 | 94 | 24 | 0 | 3 | 96 | 601 | 694 | 1.45e-44 | 152 |
| MS.gene027372.t1 | MTR_5g013420 | 63.043 | 92 | 34 | 0 | 5 | 96 | 998 | 1089 | 3.56e-34 | 123 |
| MS.gene027372.t1 | MTR_4g010830 | 58.065 | 93 | 39 | 0 | 4 | 96 | 888 | 980 | 1.34e-33 | 122 |
| MS.gene027372.t1 | MTR_5g013420 | 61.957 | 92 | 35 | 0 | 5 | 96 | 998 | 1089 | 5.83e-33 | 120 |
| MS.gene027372.t1 | MTR_4g010550 | 59.551 | 89 | 36 | 0 | 8 | 96 | 376 | 464 | 8.64e-33 | 118 |
| MS.gene027372.t1 | MTR_7g445710 | 50.562 | 89 | 44 | 0 | 8 | 96 | 455 | 543 | 4.09e-27 | 103 |
| MS.gene027372.t1 | MTR_7g450730 | 47.191 | 89 | 47 | 0 | 8 | 96 | 417 | 505 | 2.92e-24 | 95.1 |
| MS.gene027372.t1 | MTR_8g078490 | 43.750 | 96 | 47 | 1 | 1 | 96 | 1165 | 1253 | 3.85e-18 | 78.2 |
| MS.gene027372.t1 | MTR_3g011440 | 45.238 | 84 | 45 | 1 | 10 | 92 | 577 | 660 | 9.06e-18 | 77.0 |
| MS.gene027372.t1 | MTR_1g048950 | 44.318 | 88 | 44 | 1 | 9 | 96 | 451 | 533 | 2.17e-17 | 75.9 |
| MS.gene027372.t1 | MTR_7g445600 | 54.237 | 59 | 27 | 0 | 7 | 65 | 297 | 355 | 2.42e-17 | 75.1 |
| MS.gene027372.t1 | MTR_8g042750 | 42.857 | 84 | 47 | 1 | 10 | 92 | 601 | 684 | 4.31e-17 | 75.1 |
| MS.gene027372.t1 | MTR_4g132610 | 42.708 | 96 | 48 | 1 | 1 | 96 | 959 | 1047 | 5.46e-17 | 74.7 |
| MS.gene027372.t1 | MTR_7g445530 | 55.172 | 58 | 26 | 0 | 8 | 65 | 455 | 512 | 1.01e-16 | 73.9 |
| MS.gene027372.t1 | MTR_5g018850 | 41.667 | 96 | 49 | 1 | 1 | 96 | 1419 | 1507 | 4.15e-16 | 72.4 |
| MS.gene027372.t1 | MTR_1g012890 | 38.462 | 91 | 46 | 2 | 5 | 95 | 287 | 367 | 1.39e-14 | 67.8 |
| MS.gene027372.t1 | MTR_7g088370 | 38.554 | 83 | 47 | 1 | 10 | 92 | 623 | 701 | 4.60e-14 | 66.2 |
| MS.gene027372.t1 | MTR_8g078505 | 41.758 | 91 | 45 | 2 | 1 | 91 | 451 | 533 | 1.16e-13 | 65.1 |
| MS.gene027372.t1 | MTR_1g069570 | 42.857 | 91 | 47 | 3 | 4 | 92 | 655 | 742 | 1.27e-13 | 65.1 |
| MS.gene027372.t1 | MTR_1g080340 | 38.298 | 94 | 54 | 2 | 2 | 94 | 494 | 584 | 9.13e-13 | 62.8 |
| MS.gene027372.t1 | MTR_1g012910 | 35.165 | 91 | 49 | 2 | 5 | 95 | 590 | 670 | 9.08e-12 | 59.7 |
| MS.gene027372.t1 | MTR_1g012910 | 35.165 | 91 | 49 | 2 | 5 | 95 | 427 | 507 | 1.01e-11 | 59.7 |
| MS.gene027372.t1 | MTR_8g070075 | 40.323 | 62 | 37 | 0 | 5 | 66 | 688 | 749 | 1.67e-11 | 58.9 |
| MS.gene027372.t1 | MTR_7g117355 | 35.484 | 93 | 52 | 2 | 1 | 93 | 877 | 961 | 2.68e-11 | 58.5 |
| MS.gene027372.t1 | MTR_8g070070 | 41.667 | 60 | 35 | 0 | 7 | 66 | 697 | 756 | 3.25e-11 | 58.2 |
| MS.gene027372.t1 | MTR_8g071030 | 38.710 | 62 | 38 | 0 | 7 | 68 | 677 | 738 | 4.52e-11 | 57.8 |
| MS.gene027372.t1 | MTR_6g059310 | 36.842 | 95 | 48 | 3 | 1 | 95 | 168 | 250 | 5.29e-11 | 57.4 |
| MS.gene027372.t1 | MTR_1g035420 | 36.765 | 68 | 43 | 0 | 7 | 74 | 586 | 653 | 5.45e-11 | 57.8 |
| MS.gene027372.t1 | MTR_6g059310 | 36.842 | 95 | 48 | 3 | 1 | 95 | 168 | 250 | 5.81e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027372.t1 | AT5G13960 | 76.596 | 94 | 22 | 0 | 3 | 96 | 397 | 490 | 1.19e-46 | 155 |
| MS.gene027372.t1 | AT5G13960 | 76.596 | 94 | 22 | 0 | 3 | 96 | 531 | 624 | 3.52e-46 | 156 |
| MS.gene027372.t1 | AT2G22740 | 57.303 | 89 | 38 | 0 | 8 | 96 | 702 | 790 | 1.03e-29 | 110 |
| MS.gene027372.t1 | AT2G22740 | 57.303 | 89 | 38 | 0 | 8 | 96 | 702 | 790 | 1.03e-29 | 110 |
| MS.gene027372.t1 | AT2G35160 | 48.958 | 96 | 49 | 0 | 1 | 96 | 699 | 794 | 1.13e-27 | 105 |
| MS.gene027372.t1 | AT2G35160 | 48.958 | 96 | 49 | 0 | 1 | 96 | 699 | 794 | 1.13e-27 | 105 |
| MS.gene027372.t1 | AT2G35160 | 48.958 | 96 | 49 | 0 | 1 | 96 | 699 | 794 | 1.13e-27 | 105 |
| MS.gene027372.t1 | AT2G35160 | 48.958 | 96 | 49 | 0 | 1 | 96 | 699 | 794 | 1.13e-27 | 105 |
| MS.gene027372.t1 | AT2G35160 | 48.958 | 96 | 49 | 0 | 1 | 96 | 663 | 758 | 1.15e-27 | 105 |
| MS.gene027372.t1 | AT1G73100 | 42.857 | 84 | 47 | 1 | 10 | 92 | 582 | 665 | 1.01e-16 | 73.9 |
| MS.gene027372.t1 | AT5G04940 | 41.667 | 84 | 48 | 1 | 10 | 92 | 583 | 666 | 6.78e-15 | 68.9 |
| MS.gene027372.t1 | AT5G04940 | 41.667 | 84 | 48 | 1 | 10 | 92 | 583 | 666 | 6.78e-15 | 68.9 |
| MS.gene027372.t1 | AT2G23740 | 38.947 | 95 | 54 | 1 | 1 | 95 | 1284 | 1374 | 6.88e-15 | 68.9 |
| MS.gene027372.t1 | AT2G23740 | 38.947 | 95 | 54 | 1 | 1 | 95 | 1291 | 1381 | 7.22e-15 | 68.9 |
| MS.gene027372.t1 | AT2G23740 | 38.947 | 95 | 54 | 1 | 1 | 95 | 1291 | 1381 | 7.22e-15 | 68.9 |
| MS.gene027372.t1 | AT2G23740 | 38.947 | 95 | 54 | 1 | 1 | 95 | 1291 | 1381 | 7.22e-15 | 68.9 |
| MS.gene027372.t1 | AT2G24740 | 43.023 | 86 | 46 | 2 | 10 | 92 | 666 | 751 | 6.73e-14 | 65.9 |
| MS.gene027372.t1 | AT1G17770 | 41.860 | 86 | 47 | 1 | 10 | 92 | 604 | 689 | 2.49e-13 | 64.3 |
| MS.gene027372.t1 | AT4G13460 | 43.750 | 64 | 36 | 0 | 7 | 70 | 578 | 641 | 2.52e-12 | 61.6 |
| MS.gene027372.t1 | AT4G13460 | 43.750 | 64 | 36 | 0 | 7 | 70 | 578 | 641 | 2.52e-12 | 61.6 |
| MS.gene027372.t1 | AT1G77300 | 40.476 | 84 | 40 | 3 | 9 | 92 | 1090 | 1163 | 4.14e-11 | 58.2 |
| MS.gene027372.t1 | AT1G77300 | 40.476 | 84 | 40 | 3 | 9 | 92 | 1090 | 1163 | 4.95e-11 | 57.8 |
Find 18 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCATAGATGCTGGTTCTTT+TGG | 0.238650 | 7.4:-50537675 | MS.gene027372:CDS |
| CCAGCATCTATGCAATATTC+AGG | 0.354345 | 7.4:+50537684 | None:intergenic |
| AGAAGACATACCTCCCTATC+AGG | 0.387240 | 7.4:-50537544 | MS.gene027372:intron |
| CAGGAACTCACATATGACTA+TGG | 0.430156 | 7.4:-50537347 | MS.gene027372:CDS |
| ACACATTGTACAAAGAGGTT+TGG | 0.458185 | 7.4:+50537618 | None:intergenic |
| TACAAAGAGGAATACCTGAT+AGG | 0.463396 | 7.4:+50537530 | None:intergenic |
| ACAAAGAGGAATACCTGATA+GGG | 0.490411 | 7.4:+50537531 | None:intergenic |
| TACACACTCGATAGTGTGTC+TGG | 0.507324 | 7.4:-50537323 | MS.gene027372:CDS |
| GTTTCAAGATTTATCAACCA+TGG | 0.524532 | 7.4:-50537648 | MS.gene027372:CDS |
| CCTGAATATTGCATAGATGC+TGG | 0.535805 | 7.4:-50537684 | MS.gene027372:CDS |
| GATAGTGTGTCTGGTTCTGA+TGG | 0.539465 | 7.4:-50537314 | MS.gene027372:CDS |
| AGAGGTTTGGCTCACATCCA+TGG | 0.542639 | 7.4:+50537631 | None:intergenic |
| ACAATGTCATTGTGGTTCAA+AGG | 0.554813 | 7.4:-50537276 | MS.gene027372:CDS |
| TGAGAACACATTGTACAAAG+AGG | 0.579762 | 7.4:+50537613 | None:intergenic |
| AGCACTACACGAGCTAGTCT+AGG | 0.619841 | 7.4:+50537576 | None:intergenic |
| AAACAGCTACAATGTCATTG+TGG | 0.648614 | 7.4:-50537284 | MS.gene027372:CDS |
| AAGAGGAATACCTGATAGGG+AGG | 0.649275 | 7.4:+50537534 | None:intergenic |
| GAGCTAGTCTAGGATCTCTG+TGG | 0.733830 | 7.4:+50537586 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTAAAAATTTAATAAACAAT+TGG | - | chr7.4:50537565-50537584 | MS.gene027372:CDS | 10.0% |
| !!! | TGTTTTTTTTTTAAACATGA+TGG | - | chr7.4:50537497-50537516 | MS.gene027372:intron | 15.0% |
| !!! | TTATTAAATTTTTACCATGA+AGG | + | chr7.4:50537560-50537579 | None:intergenic | 15.0% |
| !! | GGTATATTTTCTAACTTAAC+AGG | - | chr7.4:50537586-50537605 | MS.gene027372:CDS | 25.0% |
| !! | TTTTATGATAACTTCCTTCA+TGG | - | chr7.4:50537543-50537562 | MS.gene027372:intron | 25.0% |
| !!! | GGTTTGTTATTGCATATATA+TGG | - | chr7.4:50537518-50537537 | MS.gene027372:intron | 25.0% |
| AGGTATTCCTCTTTGTAATA+AGG | - | chr7.4:50537428-50537447 | MS.gene027372:intron | 30.0% | |
| CTAAGATCCTTATTACAAAG+AGG | + | chr7.4:50537438-50537457 | None:intergenic | 30.0% | |
| CTCTTTGTAATAAGGATCTT+AGG | - | chr7.4:50537436-50537455 | MS.gene027372:intron | 30.0% | |
| GTTTCAAGATTTATCAACCA+TGG | - | chr7.4:50537304-50537323 | MS.gene027372:CDS | 30.0% | |
| AAACAGCTACAATGTCATTG+TGG | - | chr7.4:50537668-50537687 | MS.gene027372:CDS | 35.0% | |
| ACAAAGAGGAATACCTGATA+GGG | + | chr7.4:50537424-50537443 | None:intergenic | 35.0% | |
| ACAATGTCATTGTGGTTCAA+AGG | - | chr7.4:50537676-50537695 | MS.gene027372:CDS | 35.0% | |
| ACACATTGTACAAAGAGGTT+TGG | + | chr7.4:50537337-50537356 | None:intergenic | 35.0% | |
| TACAAAGAGGAATACCTGAT+AGG | + | chr7.4:50537425-50537444 | None:intergenic | 35.0% | |
| TGAGAACACATTGTACAAAG+AGG | + | chr7.4:50537342-50537361 | None:intergenic | 35.0% | |
| CAGGAACTCACATATGACTA+TGG | - | chr7.4:50537605-50537624 | MS.gene027372:CDS | 40.0% | |
| CCAGCATCTATGCAATATTC+AGG | + | chr7.4:50537271-50537290 | None:intergenic | 40.0% | |
| CCTGAATATTGCATAGATGC+TGG | - | chr7.4:50537268-50537287 | MS.gene027372:CDS | 40.0% | |
| !!! | TGCATAGATGCTGGTTCTTT+TGG | - | chr7.4:50537277-50537296 | MS.gene027372:CDS | 40.0% |
| AAGAGGAATACCTGATAGGG+AGG | + | chr7.4:50537421-50537440 | None:intergenic | 45.0% | |
| AGAAGACATACCTCCCTATC+AGG | - | chr7.4:50537408-50537427 | MS.gene027372:intron | 45.0% | |
| TACACACTCGATAGTGTGTC+TGG | - | chr7.4:50537629-50537648 | MS.gene027372:CDS | 45.0% | |
| !! | GATAGTGTGTCTGGTTCTGA+TGG | - | chr7.4:50537638-50537657 | MS.gene027372:CDS | 45.0% |
| ! | AGAGGTTTGGCTCACATCCA+TGG | + | chr7.4:50537324-50537343 | None:intergenic | 50.0% |
| ! | AGCACTACACGAGCTAGTCT+AGG | + | chr7.4:50537379-50537398 | None:intergenic | 50.0% |
| ! | GAGCTAGTCTAGGATCTCTG+TGG | + | chr7.4:50537369-50537388 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 50537253 | 50537721 | 50537253 | ID=MS.gene027372 |
| chr7.4 | mRNA | 50537253 | 50537721 | 50537253 | ID=MS.gene027372.t1;Parent=MS.gene027372 |
| chr7.4 | exon | 50537545 | 50537721 | 50537545 | ID=MS.gene027372.t1.exon1;Parent=MS.gene027372.t1 |
| chr7.4 | CDS | 50537545 | 50537721 | 50537545 | ID=cds.MS.gene027372.t1;Parent=MS.gene027372.t1 |
| chr7.4 | exon | 50537253 | 50537366 | 50537253 | ID=MS.gene027372.t1.exon2;Parent=MS.gene027372.t1 |
| chr7.4 | CDS | 50537253 | 50537366 | 50537253 | ID=cds.MS.gene027372.t1;Parent=MS.gene027372.t1 |
| Gene Sequence |
| Protein sequence |
