Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027437.t1 | RYR21771.1 | 61.1 | 909 | 236 | 12 | 71 | 910 | 3 | 862 | 1.40E-267 | 932.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027437.t1 | Q6H7U2 | 30.6 | 447 | 251 | 15 | 75 | 493 | 11 | 426 | 2.7e-33 | 145.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027437.t1 | A0A445A5N9 | 61.1 | 909 | 236 | 12 | 71 | 910 | 3 | 862 | 1.0e-267 | 932.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene027437.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027437.t1 | MTR_4g046697 | 95.395 | 456 | 20 | 1 | 52 | 507 | 7 | 461 | 0.0 | 897 |
| MS.gene027437.t1 | MTR_4g046697 | 95.909 | 440 | 17 | 1 | 68 | 507 | 1 | 439 | 0.0 | 872 |
| MS.gene027437.t1 | MTR_4g046697 | 95.718 | 397 | 16 | 1 | 111 | 507 | 19 | 414 | 0.0 | 776 |
| MS.gene027437.t1 | MTR_4g046707 | 96.111 | 360 | 12 | 1 | 551 | 910 | 1 | 358 | 0.0 | 713 |
| MS.gene027437.t1 | MTR_4g046697 | 96.839 | 348 | 11 | 0 | 68 | 415 | 1 | 348 | 0.0 | 694 |
| MS.gene027437.t1 | MTR_4g046697 | 96.721 | 305 | 10 | 0 | 111 | 415 | 19 | 323 | 0.0 | 599 |
| MS.gene027437.t1 | MTR_4g046697 | 93.640 | 283 | 17 | 1 | 225 | 507 | 34 | 315 | 0.0 | 541 |
| MS.gene027437.t1 | MTR_6g055230 | 48.443 | 289 | 108 | 3 | 621 | 905 | 46 | 297 | 2.78e-75 | 249 |
| MS.gene027437.t1 | MTR_4g046663 | 69.737 | 76 | 23 | 0 | 814 | 889 | 1 | 76 | 8.64e-31 | 116 |
| MS.gene027437.t1 | MTR_1g101540 | 29.258 | 229 | 141 | 5 | 650 | 859 | 93 | 319 | 1.04e-24 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027437.t1 | AT1G05270 | 59.043 | 376 | 133 | 4 | 551 | 910 | 1 | 371 | 1.71e-150 | 449 |
| MS.gene027437.t1 | AT2G32340 | 56.686 | 344 | 132 | 5 | 580 | 907 | 13 | 355 | 6.05e-126 | 384 |
| MS.gene027437.t1 | AT2G32340 | 62.097 | 248 | 86 | 4 | 586 | 831 | 38 | 279 | 6.83e-98 | 308 |
| MS.gene027437.t1 | AT3G21810 | 37.671 | 292 | 167 | 4 | 75 | 351 | 7 | 298 | 5.86e-44 | 166 |
| MS.gene027437.t1 | AT3G21810 | 36.709 | 237 | 147 | 1 | 118 | 351 | 38 | 274 | 9.66e-32 | 129 |
| MS.gene027437.t1 | AT5G52030 | 31.148 | 244 | 132 | 7 | 650 | 859 | 94 | 335 | 7.62e-21 | 96.7 |
| MS.gene027437.t1 | AT5G52030 | 34.965 | 143 | 87 | 4 | 721 | 859 | 51 | 191 | 9.50e-16 | 78.6 |
Find 215 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATGTTTGAAGTACTTTA+TGG | 0.122060 | 4.2:-53855295 | MS.gene027437:CDS |
| TCCAATCACCAAACATAATT+TGG | 0.129454 | 4.2:-53862580 | MS.gene027437:CDS |
| GATACTTCAATTCCATTATC+AGG | 0.154544 | 4.2:+53861699 | None:intergenic |
| AGTAAGCTTGAGGTCTTTCC+TGG | 0.209500 | 4.2:-53855111 | MS.gene027437:CDS |
| TAAAGAGTTTCCAACTTTAA+TGG | 0.223585 | 4.2:-53854030 | MS.gene027437:CDS |
| GTTCCTGAATTCTAGAATTA+AGG | 0.245226 | 4.2:+53863094 | None:intergenic |
| TCTGTAGGTGTTGGCGTGGC+TGG | 0.248845 | 4.2:-53853153 | MS.gene027437:CDS |
| TAGCCTTAATTCTAGAATTC+AGG | 0.264978 | 4.2:-53863097 | MS.gene027437:CDS |
| TCGAATGCACAATCAGAAAT+TGG | 0.265489 | 4.2:-53862873 | MS.gene027437:CDS |
| AAGATAGATGCCTGATATTA+TGG | 0.276412 | 4.2:+53853125 | None:intergenic |
| GCGGATCTCCTTGCAGAAAA+AGG | 0.278872 | 4.2:-53862548 | MS.gene027437:CDS |
| GTAAGGTGTGGGTGCGGCGG+AGG | 0.297036 | 4.2:+53858523 | None:intergenic |
| TTCTCAGACATTTCTAGAAT+TGG | 0.297684 | 4.2:-53862663 | MS.gene027437:CDS |
| TTCTGAATGAATGTTGTTAG+AGG | 0.301622 | 4.2:+53861629 | None:intergenic |
| TGGTGTTTGTGATGTATATT+TGG | 0.309375 | 4.2:-53858211 | MS.gene027437:CDS |
| TGATAATGGAATGGCATTAC+AGG | 0.320982 | 4.2:-53861261 | MS.gene027437:CDS |
| GTGTGTTGTTGCTGAGAAAA+AGG | 0.322313 | 4.2:+53869306 | None:intergenic |
| AAGAGGATGGGGAGTATAAA+AGG | 0.327357 | 4.2:-53863053 | MS.gene027437:intron |
| AAGTCTTCGGCGGCGACGGC+GGG | 0.329889 | 4.2:+53858459 | None:intergenic |
| TTGCTGCGGGGAATCAAATC+TGG | 0.330633 | 4.2:+53861212 | None:intergenic |
| TACAAGGAATAAAGAAGAAT+TGG | 0.330803 | 4.2:-53853450 | MS.gene027437:CDS |
| CTGTAGGTGTTGGCGTGGCT+GGG | 0.344238 | 4.2:-53853152 | MS.gene027437:CDS |
| CATTGCAGAGATAGCAGGTT+TGG | 0.351328 | 4.2:+53853188 | None:intergenic |
| GGAGGGTTGTTGAGAAGAAT+TGG | 0.355194 | 4.2:+53858541 | None:intergenic |
| TGTGAGGAGGCCCCTGATAA+TGG | 0.359411 | 4.2:-53861711 | MS.gene027437:CDS |
| GCAGGAAAGAGAGACTATTT+AGG | 0.365279 | 4.2:-53866806 | MS.gene027437:intron |
| TAGAAATACTCTTGAGGATC+AGG | 0.371223 | 4.2:-53864617 | MS.gene027437:intron |
| GTGGTAGAGGAGGAATCTGG+TGG | 0.373469 | 4.2:+53858483 | None:intergenic |
| TGAAAATTCACCATTATAGT+TGG | 0.375369 | 4.2:+53869119 | None:intergenic |
| ACTTTGGTACAAGACAAAAT+TGG | 0.377559 | 4.2:-53854262 | MS.gene027437:CDS |
| TCACATCCACGATCTCAAAA+TGG | 0.378158 | 4.2:-53858433 | MS.gene027437:CDS |
| GCTTACCCTTCAAAATCTTA+AGG | 0.379693 | 4.2:-53856310 | MS.gene027437:intron |
| AGTGGCCAAAGTGATATTTC+TGG | 0.381454 | 4.2:-53864706 | MS.gene027437:CDS |
| GGTTGACGGCACTTCAATCC+TGG | 0.385159 | 4.2:+53861370 | None:intergenic |
| GAACTTACTGATCTCGTTCA+TGG | 0.387801 | 4.2:+53853997 | None:intergenic |
| GAAAACACAAGAAAGGAATA+TGG | 0.388574 | 4.2:-53861656 | MS.gene027437:CDS |
| TCAAGGACAGAAAATGATAA+TGG | 0.388686 | 4.2:-53861275 | MS.gene027437:CDS |
| TCAATCATTTCTCTCATAGT+AGG | 0.391823 | 4.2:+53855337 | None:intergenic |
| TCAATCATCCAGTCTGTTCA+TGG | 0.395120 | 4.2:-53871090 | MS.gene027437:CDS |
| AGGAAAGACCTCAAGCTTAC+TGG | 0.396406 | 4.2:+53855113 | None:intergenic |
| GCTGGGGTTGCCATAATATC+AGG | 0.399879 | 4.2:-53853135 | MS.gene027437:CDS |
| TATGAAGAAGCAATTAAGTA+TGG | 0.408284 | 4.2:-53855069 | MS.gene027437:CDS |
| GAGGGTTGTTGAGAAGAATT+GGG | 0.408679 | 4.2:+53858542 | None:intergenic |
| AAAATGTATCCAACCTGTAC+AGG | 0.418915 | 4.2:+53855021 | None:intergenic |
| GCGTCGTCTGCTCCTGCATC+AGG | 0.418956 | 4.2:+53858333 | None:intergenic |
| TCCAAATTATGTTTGGTGAT+TGG | 0.421141 | 4.2:+53862579 | None:intergenic |
| ATGAATGAAAGGCGCGAGTT+GGG | 0.425930 | 4.2:+53858593 | None:intergenic |
| GAAGTCTTCGGCGGCGACGG+CGG | 0.427792 | 4.2:+53858458 | None:intergenic |
| GTCTTCGGCGGCGACGGCGG+GGG | 0.442053 | 4.2:+53858461 | None:intergenic |
| CACGACTCGAGCACAATTCA+AGG | 0.450223 | 4.2:+53856340 | None:intergenic |
| TCTGGTGGGTGTGCGTCCAT+TGG | 0.451428 | 4.2:+53858498 | None:intergenic |
| GTTTGTGATGTATATTTGGT+TGG | 0.452790 | 4.2:-53858207 | MS.gene027437:CDS |
| CAGAAGACATAGGAGAAAAC+AGG | 0.454769 | 4.2:-53864734 | MS.gene027437:CDS |
| GTAAGCAATGGTGAGAATAT+TGG | 0.456158 | 4.2:-53862609 | MS.gene027437:CDS |
| GACAAAACTAACCGATAGTT+GGG | 0.456546 | 4.2:+53864448 | None:intergenic |
| GTTCTGTTCCAAATTATGTT+TGG | 0.459172 | 4.2:+53862572 | None:intergenic |
| CATGTGCAAAAGAACAGTTC+TGG | 0.459711 | 4.2:+53869169 | None:intergenic |
| ATTGCAGAGATAGCAGGTTT+GGG | 0.462199 | 4.2:+53853189 | None:intergenic |
| TCGGATCGATGATCATTCTT+CGG | 0.462677 | 4.2:-53858388 | MS.gene027437:CDS |
| TCTCGAGCCGGTGAATATCT+TGG | 0.463968 | 4.2:+53866737 | None:intergenic |
| TTTAGGTAATAATGACTTGA+GGG | 0.469756 | 4.2:-53866789 | MS.gene027437:CDS |
| AGACAAAACTAACCGATAGT+TGG | 0.470883 | 4.2:+53864447 | None:intergenic |
| GGGGTGGTAGAGGAGGAATC+TGG | 0.472253 | 4.2:+53858480 | None:intergenic |
| AGTCTTCGGCGGCGACGGCG+GGG | 0.472313 | 4.2:+53858460 | None:intergenic |
| TAAGGTGTGGGTGCGGCGGA+GGG | 0.472774 | 4.2:+53858524 | None:intergenic |
| TTATGGCTGGTTCCTTGCCA+AGG | 0.479240 | 4.2:-53855278 | MS.gene027437:intron |
| ATAATGGAATTGAAGTATCT+AGG | 0.479355 | 4.2:-53861695 | MS.gene027437:CDS |
| AGATCAGTTGTGGCTGTTGT+TGG | 0.480034 | 4.2:-53853484 | MS.gene027437:CDS |
| GTCTTTACATCTGTAGGTGT+TGG | 0.482799 | 4.2:-53853162 | MS.gene027437:CDS |
| TCGTCTGCTCCTCCTGATGC+AGG | 0.483156 | 4.2:-53858345 | MS.gene027437:CDS |
| AAAGTACTTCAAACATGTTA+CGG | 0.487465 | 4.2:+53855299 | None:intergenic |
| CACCCACACCTTACCTCCAA+TGG | 0.488447 | 4.2:-53858514 | MS.gene027437:CDS |
| GAGATAGCAGGTTTGGGAGA+TGG | 0.488938 | 4.2:+53853195 | None:intergenic |
| GTTATTAAGATCATCAGAGC+TGG | 0.494439 | 4.2:+53854206 | None:intergenic |
| AATGAAGTCTTCGGCGGCGA+CGG | 0.495317 | 4.2:+53858455 | None:intergenic |
| ACAACACACTCGTCGCAACT+TGG | 0.496083 | 4.2:-53869292 | MS.gene027437:CDS |
| CGACCTCTTGCCTCTCGAGC+CGG | 0.496526 | 4.2:+53866725 | None:intergenic |
| TCTTGTGAATCTTCTGCTGA+AGG | 0.497214 | 4.2:-53858234 | MS.gene027437:CDS |
| ATACTTCAATTCCATTATCA+GGG | 0.497610 | 4.2:+53861700 | None:intergenic |
| GCTTCTTCAGATGAAGGAGA+TGG | 0.498793 | 4.2:-53854093 | MS.gene027437:intron |
| TGCTGCGGGGAATCAAATCT+GGG | 0.499304 | 4.2:+53861213 | None:intergenic |
| TTCTAGAATTCAGGAACTTG+AGG | 0.500849 | 4.2:-53863088 | MS.gene027437:CDS |
| ACAAGGGTCTCCATTAAAGT+TGG | 0.503321 | 4.2:+53854020 | None:intergenic |
| TAACAACATTCATTCAGAAA+AGG | 0.504454 | 4.2:-53861625 | MS.gene027437:intron |
| CAGGTCCGACTTCAAAGGTT+CGG | 0.509285 | 4.2:-53862696 | MS.gene027437:CDS |
| ACTTCAATCCTGGATTCCTT+CGG | 0.509539 | 4.2:+53861380 | None:intergenic |
| ACTCTCATTGCAGAGATAGC+AGG | 0.509564 | 4.2:+53853183 | None:intergenic |
| TCCCCGCAGCAATTATTCAC+AGG | 0.509785 | 4.2:-53861201 | MS.gene027437:intron |
| GTTACCGTTGTGTTTATCAT+CGG | 0.510198 | 4.2:-53871028 | MS.gene027437:CDS |
| TGTTTGAAGTACTTTATGGC+TGG | 0.512196 | 4.2:-53855291 | MS.gene027437:CDS |
| AGCGTTGATATTGTAAGCAA+TGG | 0.513750 | 4.2:-53862621 | MS.gene027437:CDS |
| CTGATACTAATGAAACTTCA+AGG | 0.514385 | 4.2:-53861292 | MS.gene027437:CDS |
| AAGCAATTAAGTATGGTGGC+AGG | 0.514395 | 4.2:-53855062 | MS.gene027437:CDS |
| TGTAGGTGTTGGCGTGGCTG+GGG | 0.514441 | 4.2:-53853151 | MS.gene027437:CDS |
| GTCCGCCACATTATTAAAAG+TGG | 0.514526 | 4.2:-53853518 | MS.gene027437:CDS |
| GTAACCACCTTCGAGGTAGC+TGG | 0.514586 | 4.2:+53871046 | None:intergenic |
| AACTTCCAGAAATATCACTT+TGG | 0.517782 | 4.2:+53864701 | None:intergenic |
| CTCTGATGATCTTAATAACA+TGG | 0.517890 | 4.2:-53854202 | MS.gene027437:intron |
| ATATACAGCCGAAGGAATCC+AGG | 0.519449 | 4.2:-53861388 | MS.gene027437:intron |
| AAATTATGTTTGGTGATTGG+AGG | 0.521526 | 4.2:+53862582 | None:intergenic |
| GTGTGCGTCCATTGGAGGTA+AGG | 0.525018 | 4.2:+53858506 | None:intergenic |
| AACCTGTGAATAATTGCTGC+GGG | 0.525149 | 4.2:+53861199 | None:intergenic |
| GTTGATATTGAGCATGATAA+CGG | 0.525710 | 4.2:-53861317 | MS.gene027437:CDS |
| TTGGAGGTAAGGTGTGGGTG+CGG | 0.526482 | 4.2:+53858517 | None:intergenic |
| CTCAGTTAAACAAAGAGGAT+GGG | 0.527683 | 4.2:-53863065 | MS.gene027437:CDS |
| ATCACCTCCAAGATATTCAC+CGG | 0.528176 | 4.2:-53866744 | MS.gene027437:CDS |
| TGGAATGGCATTACAGGTGA+AGG | 0.528941 | 4.2:-53861255 | MS.gene027437:CDS |
| GTGGATGTGAATGAAGTCTT+CGG | 0.532966 | 4.2:+53858446 | None:intergenic |
| TGAATCCACCGCTGCGACGA+CGG | 0.534633 | 4.2:+53858311 | None:intergenic |
| GAATGTTCACATACTATGAC+AGG | 0.534672 | 4.2:+53853421 | None:intergenic |
| ACCTTCGAGGTAGCTGGCAG+CGG | 0.535764 | 4.2:+53871052 | None:intergenic |
| CAATAATTCCATGAACAGAC+TGG | 0.536995 | 4.2:+53871082 | None:intergenic |
| CGACGCCGTCGTCGCAGCGG+TGG | 0.537022 | 4.2:-53858316 | MS.gene027437:CDS |
| TTCTAGAATTGGTGCTGATG+AGG | 0.537743 | 4.2:-53862652 | MS.gene027437:CDS |
| TGAGGTCTCCAGAGAAAAGA+AGG | 0.539215 | 4.2:-53866262 | MS.gene027437:intron |
| GCTCAGTTAAACAAAGAGGA+TGG | 0.540853 | 4.2:-53863066 | MS.gene027437:CDS |
| TCCTGGGTCTGAGTTTCGTG+TGG | 0.541592 | 4.2:-53855094 | MS.gene027437:CDS |
| CATGGAGTAAGATGCCACTT+TGG | 0.542574 | 4.2:-53854278 | MS.gene027437:CDS |
| ACTTGGTGACCGCCCTGTAC+AGG | 0.542788 | 4.2:-53855034 | MS.gene027437:intron |
| GAGGTAAGGTGTGGGTGCGG+CGG | 0.546902 | 4.2:+53858520 | None:intergenic |
| GGAGTTGAGTGCGAGTGAGG+TGG | 0.547101 | 4.2:+53858616 | None:intergenic |
| GGTGGGTGTGCGTCCATTGG+AGG | 0.548226 | 4.2:+53858501 | None:intergenic |
| ACCGTTGTGTTTATCATCGG+AGG | 0.549225 | 4.2:-53871025 | MS.gene027437:intron |
| TGGTAGAGGAGGAATCTGGT+GGG | 0.549256 | 4.2:+53858484 | None:intergenic |
| TGAATGAAAGGCGCGAGTTG+GGG | 0.550550 | 4.2:+53858594 | None:intergenic |
| GATGAATGAAAGGCGCGAGT+TGG | 0.551408 | 4.2:+53858592 | None:intergenic |
| AGAGGTCGGCAGACCATTCG+CGG | 0.553269 | 4.2:-53866710 | MS.gene027437:intron |
| GCTTATCCGATAGCATGGTT+CGG | 0.555726 | 4.2:-53858407 | MS.gene027437:CDS |
| CTTCTTGAACACTCTCATCC+AGG | 0.556266 | 4.2:+53863124 | None:intergenic |
| ATCTTGTGTCTAACCGCGAA+TGG | 0.556846 | 4.2:+53866697 | None:intergenic |
| ACATTCCTCGATAACTCCTC+AGG | 0.557097 | 4.2:+53858267 | None:intergenic |
| CGAGCCGGTGAATATCTTGG+AGG | 0.558434 | 4.2:+53866740 | None:intergenic |
| CCACAGAATCTCGTTCTGCA+TGG | 0.561131 | 4.2:+53862523 | None:intergenic |
| GTAAGCTTGAGGTCTTTCCT+GGG | 0.562244 | 4.2:-53855110 | MS.gene027437:CDS |
| GACAGAAAATGATAATGGAA+TGG | 0.562419 | 4.2:-53861270 | MS.gene027437:CDS |
| TCAACTTTGAACAATTCGAT+CGG | 0.563735 | 4.2:+53869262 | None:intergenic |
| AGTTGTGGCTGTTGTTGGAA+AGG | 0.565891 | 4.2:-53853479 | MS.gene027437:CDS |
| GTTGTGGCTGTTGTTGGAAA+GGG | 0.566868 | 4.2:-53853478 | MS.gene027437:CDS |
| AAGATATTCACCGGCTCGAG+AGG | 0.567135 | 4.2:-53866735 | MS.gene027437:CDS |
| TAACTTAATGTGCAGTATGA+GGG | 0.569651 | 4.2:-53861101 | MS.gene027437:intron |
| GATCACAGGTCCGACTTCAA+AGG | 0.571245 | 4.2:-53862701 | MS.gene027437:CDS |
| GACAACATGAGCTGCCATGC+TGG | 0.571700 | 4.2:+53861349 | None:intergenic |
| AGCAGCAGCACATGTGGACA+TGG | 0.572941 | 4.2:-53861036 | MS.gene027437:intron |
| CTTGACGGAAAACACAAGAA+AGG | 0.574193 | 4.2:-53861663 | MS.gene027437:CDS |
| TCTAGGCACCAAGATCTTGA+CGG | 0.576124 | 4.2:-53861678 | MS.gene027437:CDS |
| AGCAATTAAGTATGGTGGCA+GGG | 0.576248 | 4.2:-53855061 | MS.gene027437:CDS |
| TTGAGATGGTGATGAATGAA+AGG | 0.577954 | 4.2:+53858582 | None:intergenic |
| TGTGAATCTTCTGCTGAAGG+TGG | 0.578487 | 4.2:-53858231 | MS.gene027437:CDS |
| GGATGAGAGTGTTCAAGAAG+TGG | 0.580126 | 4.2:-53863121 | MS.gene027437:CDS |
| ACTGATCTCGTTCATGGACA+AGG | 0.581009 | 4.2:+53854003 | None:intergenic |
| TCAGTTAAACAAAGAGGATG+GGG | 0.582580 | 4.2:-53863064 | MS.gene027437:CDS |
| GAACCGAAAATCCCAACTAT+CGG | 0.583849 | 4.2:-53864459 | MS.gene027437:intron |
| CACAACGGTAACCACCTTCG+AGG | 0.583878 | 4.2:+53871039 | None:intergenic |
| GCCACACGAAACTCAGACCC+AGG | 0.583957 | 4.2:+53855093 | None:intergenic |
| GCCGCTGCCAGCTACCTCGA+AGG | 0.584623 | 4.2:-53871053 | MS.gene027437:CDS |
| ATGAGAGTCTTTACATCTGT+AGG | 0.586791 | 4.2:-53853168 | MS.gene027437:CDS |
| ATCGATCCGAACCATGCTAT+CGG | 0.589640 | 4.2:+53858401 | None:intergenic |
| TGAGCTGCCATGCTGGTTGA+CGG | 0.590544 | 4.2:+53861356 | None:intergenic |
| TCGTCTGCTCCTGCATCAGG+AGG | 0.591008 | 4.2:+53858336 | None:intergenic |
| GTTGGAAAGGGACATCTACA+AGG | 0.592838 | 4.2:-53853466 | MS.gene027437:CDS |
| TATGGTGGCAGGGTGATACT+TGG | 0.597732 | 4.2:-53855051 | MS.gene027437:CDS |
| AGGGTTGTTGAGAAGAATTG+GGG | 0.599401 | 4.2:+53858543 | None:intergenic |
| CCATGCAGAACGAGATTCTG+TGG | 0.599708 | 4.2:-53862523 | MS.gene027437:CDS |
| ATTTAGGTAATAATGACTTG+AGG | 0.602441 | 4.2:-53866790 | MS.gene027437:CDS |
| CGTCCATTGGAGGTAAGGTG+TGG | 0.604467 | 4.2:+53858511 | None:intergenic |
| TGGAACTGCTCATGTTTCTG+AGG | 0.605070 | 4.2:-53858187 | MS.gene027437:intron |
| GATCTCCGAACCTTTGAAGT+CGG | 0.606184 | 4.2:+53862691 | None:intergenic |
| CATAGAAACATGCCTTGGCA+AGG | 0.609074 | 4.2:+53855266 | None:intergenic |
| GGAGAGCTTATCCGATAGCA+TGG | 0.611123 | 4.2:-53858412 | MS.gene027437:CDS |
| TCAGTTGAAACAGCAAGAAG+CGG | 0.613051 | 4.2:-53861780 | MS.gene027437:CDS |
| TTATTAAGATCATCAGAGCT+GGG | 0.614482 | 4.2:+53854207 | None:intergenic |
| ACAGATTGCCAGTAAGCTTG+AGG | 0.615590 | 4.2:-53855121 | MS.gene027437:intron |
| TACTTCAATTCCATTATCAG+GGG | 0.616698 | 4.2:+53861701 | None:intergenic |
| AAATGTATCCAACCTGTACA+GGG | 0.617068 | 4.2:+53855022 | None:intergenic |
| CGCTTCCTGAGGAGTTATCG+AGG | 0.618132 | 4.2:-53858272 | MS.gene027437:CDS |
| AGATTACATTGAAGAGAACA+TGG | 0.619490 | 4.2:-53854296 | MS.gene027437:CDS |
| AAGAGTATTTCTAGAACCAG+AGG | 0.619782 | 4.2:+53864627 | None:intergenic |
| GAATGAAAGGCGCGAGTTGG+GGG | 0.623865 | 4.2:+53858595 | None:intergenic |
| GCTGCCAGCTACCTCGAAGG+TGG | 0.624077 | 4.2:-53871050 | MS.gene027437:CDS |
| GGAATGGCATTACAGGTGAA+GGG | 0.625351 | 4.2:-53861254 | MS.gene027437:CDS |
| TGAAGTGCCGTCAACCAGCA+TGG | 0.625663 | 4.2:-53861363 | MS.gene027437:CDS |
| TGGTTCTAGAAATACTCTTG+AGG | 0.626257 | 4.2:-53864623 | MS.gene027437:CDS |
| GAAGAAGCAATTAAGTATGG+TGG | 0.634407 | 4.2:-53855066 | MS.gene027437:CDS |
| GGCAGCTCATGTTGTCGATG+AGG | 0.635035 | 4.2:-53861342 | MS.gene027437:CDS |
| TGTCAGTTTACTGAAACCAG+AGG | 0.635790 | 4.2:-53856489 | MS.gene027437:intron |
| TGTGGATGGTCTTGATGAAG+AGG | 0.636361 | 4.2:-53861060 | MS.gene027437:CDS |
| AGAGGCAGCAGCAGCACATG+TGG | 0.636794 | 4.2:-53861042 | MS.gene027437:CDS |
| TGAGGCTCAGTTAAACAAAG+AGG | 0.640733 | 4.2:-53863070 | MS.gene027437:CDS |
| TACATCTGTAGGTGTTGGCG+TGG | 0.642917 | 4.2:-53853157 | MS.gene027437:CDS |
| GTCCATTGGAGGTAAGGTGT+GGG | 0.643371 | 4.2:+53858512 | None:intergenic |
| AGTTGCAAGATGAACTGAAG+AGG | 0.645881 | 4.2:-53862850 | MS.gene027437:intron |
| TAACCTGTGAATAATTGCTG+CGG | 0.646818 | 4.2:+53861198 | None:intergenic |
| AAGTGAGAGCAGATCAGTTG+TGG | 0.647491 | 4.2:-53853494 | MS.gene027437:CDS |
| CTGATCTCGTTCATGGACAA+GGG | 0.650040 | 4.2:+53854004 | None:intergenic |
| AACGAGAATGTGGATGTGGA+TGG | 0.650385 | 4.2:-53861074 | MS.gene027437:CDS |
| ATCGATTGACAAGAGTTGTG+AGG | 0.651602 | 4.2:-53861727 | MS.gene027437:CDS |
| AGACGACGCCGTCGTCGCAG+CGG | 0.651737 | 4.2:-53858319 | MS.gene027437:CDS |
| TCACCGGCTCGAGAGGCAAG+AGG | 0.653046 | 4.2:-53866728 | MS.gene027437:CDS |
| TTATGTGCGCTTTATCAGAA+CGG | 0.656000 | 4.2:-53869204 | MS.gene027437:CDS |
| TGAGGGATAAACTTGACAGA+AGG | 0.656295 | 4.2:-53866772 | MS.gene027437:CDS |
| CGGCTCGAGAGGCAAGAGGT+CGG | 0.660465 | 4.2:-53866724 | MS.gene027437:CDS |
| AAACAGCAAGAAGCGGAAAG+CGG | 0.668812 | 4.2:-53861773 | MS.gene027437:CDS |
| GGTGCAGTGACAGAAGACAT+AGG | 0.669837 | 4.2:-53864744 | MS.gene027437:intron |
| AGGAGAAAACAGGATATCAG+TGG | 0.680364 | 4.2:-53864724 | MS.gene027437:CDS |
| ACAGAAAGTTACGCTTCCTG+AGG | 0.681688 | 4.2:-53858283 | MS.gene027437:CDS |
| GATTGACAAGAGTTGTGAGG+AGG | 0.689653 | 4.2:-53861724 | MS.gene027437:CDS |
| CATAATTTGGAACAGAACAG+CGG | 0.694176 | 4.2:-53862567 | MS.gene027437:CDS |
| ACCTGTGAATAATTGCTGCG+GGG | 0.699773 | 4.2:+53861200 | None:intergenic |
| GCTGCGGGGAATCAAATCTG+GGG | 0.713601 | 4.2:+53861214 | None:intergenic |
| GATGTGAATGAAGTCTTCGG+CGG | 0.716744 | 4.2:+53858449 | None:intergenic |
| GGGTTGTTGAGAAGAATTGG+GGG | 0.728093 | 4.2:+53858544 | None:intergenic |
| ACCTCCGATGATAAACACAA+CGG | 0.733433 | 4.2:+53871024 | None:intergenic |
| TCTGTGGAAGAGTTACAACA+AGG | 0.739554 | 4.2:-53862507 | MS.gene027437:intron |
| TTGCAGAATATAGTCCACTG+AGG | 0.772392 | 4.2:-53866280 | MS.gene027437:intron |
| TGTATCCAACCTGTACAGGG+CGG | 0.779909 | 4.2:+53855025 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 53853110 | 53871457 | 53853110 | ID=MS.gene027437 |
| chr4.2 | mRNA | 53853110 | 53871457 | 53853110 | ID=MS.gene027437.t1;Parent=MS.gene027437 |
| chr4.2 | exon | 53871455 | 53871457 | 53871455 | ID=MS.gene027437.t1.exon1;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53871455 | 53871457 | 53871455 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53871026 | 53871117 | 53871026 | ID=MS.gene027437.t1.exon2;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53871026 | 53871117 | 53871026 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53870892 | 53870914 | 53870892 | ID=MS.gene027437.t1.exon3;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53870892 | 53870914 | 53870892 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53869130 | 53869342 | 53869130 | ID=MS.gene027437.t1.exon4;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53869130 | 53869342 | 53869130 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53866711 | 53866824 | 53866711 | ID=MS.gene027437.t1.exon5;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53866711 | 53866824 | 53866711 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53866263 | 53866296 | 53866263 | ID=MS.gene027437.t1.exon6;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53866263 | 53866296 | 53866263 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53864618 | 53864759 | 53864618 | ID=MS.gene027437.t1.exon7;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53864618 | 53864759 | 53864618 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53864460 | 53864516 | 53864460 | ID=MS.gene027437.t1.exon8;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53864460 | 53864516 | 53864460 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53863054 | 53863151 | 53863054 | ID=MS.gene027437.t1.exon9;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53863054 | 53863151 | 53863054 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53862851 | 53862922 | 53862851 | ID=MS.gene027437.t1.exon10;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53862851 | 53862922 | 53862851 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53862508 | 53862722 | 53862508 | ID=MS.gene027437.t1.exon11;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53862508 | 53862722 | 53862508 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53861626 | 53861807 | 53861626 | ID=MS.gene027437.t1.exon12;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53861626 | 53861807 | 53861626 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53861202 | 53861402 | 53861202 | ID=MS.gene027437.t1.exon13;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53861202 | 53861402 | 53861202 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53861037 | 53861108 | 53861037 | ID=MS.gene027437.t1.exon14;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53861037 | 53861108 | 53861037 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53858188 | 53858649 | 53858188 | ID=MS.gene027437.t1.exon15;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53858188 | 53858649 | 53858188 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53856490 | 53856537 | 53856490 | ID=MS.gene027437.t1.exon16;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53856490 | 53856537 | 53856490 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53856311 | 53856370 | 53856311 | ID=MS.gene027437.t1.exon17;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53856311 | 53856370 | 53856311 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53855279 | 53855362 | 53855279 | ID=MS.gene027437.t1.exon18;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53855279 | 53855362 | 53855279 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53855035 | 53855139 | 53855035 | ID=MS.gene027437.t1.exon19;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53855035 | 53855139 | 53855035 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53854203 | 53854316 | 53854203 | ID=MS.gene027437.t1.exon20;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53854203 | 53854316 | 53854203 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53854005 | 53854105 | 53854005 | ID=MS.gene027437.t1.exon21;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53854005 | 53854105 | 53854005 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53853435 | 53853546 | 53853435 | ID=MS.gene027437.t1.exon22;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53853435 | 53853546 | 53853435 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| chr4.2 | exon | 53853110 | 53853238 | 53853110 | ID=MS.gene027437.t1.exon23;Parent=MS.gene027437.t1 |
| chr4.2 | CDS | 53853110 | 53853238 | 53853110 | ID=cds.MS.gene027437.t1;Parent=MS.gene027437.t1 |
| Gene Sequence |
| Protein sequence |
