AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027456

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027456.t1 MTR_3g450850 68.269 208 66 0 9 216 79 286 7.92e-97 302
MS.gene027456.t1 MTR_4g071400 80.992 121 23 0 511 631 1 121 2.77e-63 206
MS.gene027456.t1 MTR_3g012160 96.774 93 3 0 125 217 1 93 6.15e-56 185
MS.gene027456.t1 MTR_4g035820 40.670 209 122 1 26 232 116 324 3.94e-43 166
MS.gene027456.t1 MTR_4g035820 36.129 155 83 2 365 503 326 480 8.93e-21 97.4
MS.gene027456.t1 MTR_6g051425 30.085 236 154 5 27 257 62 291 1.14e-23 103
MS.gene027456.t1 MTR_1g070355 29.665 209 144 3 14 222 104 309 2.01e-21 99.0
MS.gene027456.t1 MTR_3g095940 86.364 44 6 0 430 473 90 133 2.33e-18 82.8
MS.gene027456.t1 MTR_5g083630 65.517 58 20 0 126 183 19 76 1.34e-17 79.3
MS.gene027456.t1 MTR_6g014355 40.708 113 65 1 58 168 14 126 6.38e-16 78.6
MS.gene027456.t1 MTR_5g076070 25.128 195 139 5 24 216 93 282 1.66e-13 72.0
MS.gene027456.t1 MTR_7g446510 31.928 166 107 5 74 236 167 329 4.26e-13 71.2
MS.gene027456.t1 MTR_7g446480 31.928 166 107 5 74 236 152 314 9.14e-13 70.1
MS.gene027456.t1 MTR_8g446020 31.544 149 94 3 84 228 112 256 2.04e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027456.t1 AT1G10240 45.347 591 313 2 23 603 89 679 0.0 553
MS.gene027456.t1 AT1G10240 45.347 591 313 2 23 603 89 679 0.0 553
MS.gene027456.t1 AT5G28530 40.698 602 328 6 4 584 77 670 2.45e-153 459
MS.gene027456.t1 AT5G28530 40.698 602 328 6 4 584 77 670 2.45e-153 459
MS.gene027456.t1 AT5G28530 40.864 602 331 6 4 584 77 674 1.04e-152 458
MS.gene027456.t1 AT5G28530 35.980 592 307 7 4 584 77 607 3.93e-119 369
MS.gene027456.t1 AT5G28530 36.149 592 310 7 4 584 77 611 1.02e-118 368
MS.gene027456.t1 AT4G38180 30.597 536 338 9 23 534 113 638 1.06e-74 256
MS.gene027456.t1 AT4G15090 26.462 650 440 13 10 632 76 714 8.51e-68 238
MS.gene027456.t1 AT4G15090 26.462 650 440 13 10 632 76 714 8.51e-68 238
MS.gene027456.t1 AT4G15090 26.462 650 440 13 10 632 76 714 8.51e-68 238
MS.gene027456.t1 AT4G15090 26.462 650 440 13 10 632 76 714 8.51e-68 238
MS.gene027456.t1 AT4G15090 26.462 650 440 13 10 632 76 714 8.51e-68 238
MS.gene027456.t1 AT1G76320 26.542 535 364 8 6 527 21 539 6.15e-60 213
MS.gene027456.t1 AT1G76320 26.542 535 364 8 6 527 21 539 1.41e-59 213
MS.gene027456.t1 AT1G76320 26.542 535 364 8 6 527 21 539 1.41e-59 213
MS.gene027456.t1 AT1G76320 26.542 535 364 8 6 527 21 539 1.48e-59 213
MS.gene027456.t1 AT3G22170 26.600 500 318 11 50 531 149 617 7.12e-56 204
MS.gene027456.t1 AT3G22170 26.600 500 318 11 50 531 149 617 7.12e-56 204
MS.gene027456.t1 AT2G27110 26.336 524 345 13 63 567 111 612 8.37e-50 187
MS.gene027456.t1 AT2G27110 26.336 524 345 13 63 567 111 612 8.37e-50 187
MS.gene027456.t1 AT2G27110 27.816 435 284 10 152 567 44 467 1.38e-47 179
MS.gene027456.t1 AT3G06250 26.667 525 302 13 27 527 234 699 6.44e-46 175
MS.gene027456.t1 AT3G06250 26.667 525 302 13 27 527 234 699 6.44e-46 175
MS.gene027456.t1 AT3G06250 26.667 525 302 13 27 527 234 699 6.44e-46 175
MS.gene027456.t1 AT2G32250 25.333 375 265 4 172 532 180 553 1.25e-43 168
MS.gene027456.t1 AT2G32250 25.333 375 265 4 172 532 139 512 1.51e-43 168
MS.gene027456.t1 AT2G32250 25.333 375 265 4 172 532 139 512 1.56e-43 168
MS.gene027456.t1 AT2G32250 25.333 375 265 4 172 532 180 553 1.91e-43 168
MS.gene027456.t1 AT2G32250 25.333 375 265 4 172 532 180 553 1.91e-43 168
MS.gene027456.t1 AT2G32250 25.333 375 265 4 172 532 180 553 1.91e-43 168
MS.gene027456.t1 AT2G32250 25.333 375 265 4 172 532 180 553 1.98e-43 168
MS.gene027456.t1 AT2G32250 25.333 375 265 4 172 532 180 553 1.98e-43 168
MS.gene027456.t1 AT4G38170 27.731 357 241 4 193 533 45 400 2.81e-42 161
MS.gene027456.t1 AT4G19990 25.948 501 308 8 51 531 44 501 5.97e-42 162
MS.gene027456.t1 AT4G19990 25.948 501 308 8 51 531 101 558 8.80e-42 162
MS.gene027456.t1 AT4G19990 25.948 501 308 8 51 531 101 558 9.44e-42 162
MS.gene027456.t1 AT4G19990 25.948 501 308 8 51 531 101 558 9.44e-42 162
MS.gene027456.t1 AT4G19990 25.948 501 308 8 51 531 101 558 1.14e-41 162
MS.gene027456.t1 AT4G19990 25.948 501 308 8 51 531 101 558 1.14e-41 162
MS.gene027456.t1 AT4G19990 25.948 501 308 8 51 531 115 572 1.25e-41 162
MS.gene027456.t1 AT5G18960 25.626 519 318 15 27 527 255 723 2.76e-40 158
MS.gene027456.t1 AT5G18960 25.626 519 318 15 27 527 255 723 2.76e-40 158
MS.gene027456.t1 AT1G80010 25.323 387 238 9 179 530 289 659 3.20e-36 145
MS.gene027456.t1 AT1G52520 25.721 416 276 10 142 530 227 636 4.09e-36 145

Find 94 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCACATATTCTTGAGTTTC+TGG 0.087082 4.2:+54149384 None:intergenic
ACATCACAAACATCGTTATT+TGG 0.152623 4.2:+54149342 None:intergenic
ACCCATCCGGTCTTTATAAA+AGG 0.176532 4.2:-54151299 MS.gene027456:CDS
GCAATCCAAACTTACTTATT+AGG 0.216308 4.2:+54149141 None:intergenic
ATGATACGCCACTTGGAATA+TGG 0.219407 4.2:-54150690 MS.gene027456:CDS
TTCCTTTGCGTGGGCTTTAA+AGG 0.228847 4.2:-54150599 MS.gene027456:intron
TACTTTGATAGGATCATAAA+AGG 0.274738 4.2:-54149052 MS.gene027456:intron
ATTCGAGCATACGAATCTTT+TGG 0.279666 4.2:-54150754 MS.gene027456:CDS
CTCATGTAGGAGCAACTTAA+AGG 0.285515 4.2:+54149818 None:intergenic
TCATCACAATCATGAGTTAT+TGG 0.289585 4.2:-54151112 MS.gene027456:CDS
ACGAAATTGTCCAAACAAAA+AGG 0.298732 4.2:-54149193 MS.gene027456:CDS
GTTGACTACATGGCTAGATT+TGG 0.300626 4.2:+54149539 None:intergenic
TTGACTACATGGCTAGATTT+GGG 0.326040 4.2:+54149540 None:intergenic
GCAGCTTCAACTCCTCCTTT+TGG 0.329186 4.2:+54149270 None:intergenic
TCAAGAATATGTGATTGATT+TGG 0.332734 4.2:-54149376 MS.gene027456:CDS
TGATCCTCTTGGCCGACCTT+TGG 0.339991 4.2:+54149296 None:intergenic
TTGGTGTTGTTGTCAAAATT+AGG 0.343446 4.2:-54149947 MS.gene027456:CDS
TATTGATGAAAGTAACAAAT+TGG 0.343540 4.2:-54150806 MS.gene027456:CDS
TCAATCTCTAATTCGATGTT+TGG 0.356770 4.2:-54149490 MS.gene027456:CDS
ACTTAAGGAAGCCATTAAAA+TGG 0.358796 4.2:-54150421 MS.gene027456:CDS
AAAGTCATTCAAGATATAAA+AGG 0.358979 4.2:-54149411 MS.gene027456:CDS
AAAATCTGTCAAACTTGTAT+TGG 0.376571 4.2:+54150071 None:intergenic
AGCATGTCTGCATAATATTC+CGG 0.383529 4.2:+54149647 None:intergenic
CAAAATGCATGCTTTGTGTT+TGG 0.392144 4.2:+54150393 None:intergenic
AGATCAAGATCCCGCACTTA+AGG 0.392664 4.2:-54150436 MS.gene027456:CDS
GAGGAGTTGAAGCTGCAAAT+AGG 0.394021 4.2:-54149263 MS.gene027456:CDS
AATAGTGAATTCATATTTGA+AGG 0.396961 4.2:+54150825 None:intergenic
GATGCAGCATGTTCCTCAAT+AGG 0.398909 4.2:+54149858 None:intergenic
TGGAAAAGAGCAAGTCAATA+TGG 0.408931 4.2:+54150202 None:intergenic
TTTGCTGGGATGACAACAAC+AGG 0.410409 4.2:-54150138 MS.gene027456:CDS
GAGCATATTATGTGGGCATT+TGG 0.419517 4.2:-54150784 MS.gene027456:CDS
ATTCAATCCGAGAGTTGTAT+TGG 0.424169 4.2:-54150910 MS.gene027456:CDS
AACCAGCTTTCGACAACAAT+AGG 0.437905 4.2:+54151025 None:intergenic
CTTTCTTGTCTCCATCGAAA+AGG 0.441125 4.2:+54149573 None:intergenic
TTCTTTACCAATACAACTCT+CGG 0.441999 4.2:+54150903 None:intergenic
GGAAAACATGTTCTGATATG+AGG 0.442250 4.2:+54149879 None:intergenic
TGATACGCCACTTGGAATAT+GGG 0.449284 4.2:-54150689 MS.gene027456:CDS
TTGGGCATTAGCTTATTTGA+AGG 0.451954 4.2:-54150169 MS.gene027456:CDS
CCCATCCGGTCTTTATAAAA+GGG 0.452968 4.2:-54151298 MS.gene027456:CDS
ATCCTATTGTTGTCGAAAGC+TGG 0.455314 4.2:-54151027 MS.gene027456:CDS
ACATGCTATTCGTGTGCTAA+TGG 0.455837 4.2:-54149631 MS.gene027456:CDS
TGAATATCTTTATCTAGAAA+AGG 0.460825 4.2:+54150940 None:intergenic
CGAAAGAGAGTGGAAATCAA+TGG 0.466880 4.2:-54150253 MS.gene027456:CDS
TTGATAGGATCATAAAAGGT+TGG 0.470332 4.2:-54149048 MS.gene027456:intron
CAACATTGCAGTTGACTACA+TGG 0.482092 4.2:+54149529 None:intergenic
TTTCTTGTCTCCATCGAAAA+GGG 0.485154 4.2:+54149574 None:intergenic
GTTGGAATGTTGCGATAAGC+AGG 0.492886 4.2:+54151066 None:intergenic
TGATTAGTTCCACCACAACT+TGG 0.507884 4.2:+54149222 None:intergenic
CGCTGTCATGTTCCAAGTTG+TGG 0.511566 4.2:-54149234 MS.gene027456:CDS
TAATGCCCAAAATTCAAGAT+TGG 0.511972 4.2:+54150182 None:intergenic
CCAAGAGGATCAAGACCAAA+AGG 0.514192 4.2:-54149285 MS.gene027456:CDS
AGGAGCAACTTAAAGGCATA+TGG 0.516663 4.2:+54149825 None:intergenic
GTCAAAATTAGGAATCAAGC+TGG 0.523988 4.2:-54149936 MS.gene027456:CDS
ACGGTGGGCGTCAAGTAAGT+TGG 0.528714 4.2:-54149719 MS.gene027456:CDS
TTGTGGCAAAGTTGTCAAGT+TGG 0.535071 4.2:-54150359 MS.gene027456:CDS
ACAAATTGGAGCATATTATG+TGG 0.542960 4.2:-54150792 MS.gene027456:CDS
TAGATAACTCAATCTCATGT+AGG 0.545292 4.2:+54149805 None:intergenic
AGAAGATTTCTTCCTTTGCG+TGG 0.551128 4.2:-54150609 MS.gene027456:CDS
TAGAGATTGAAACTTAGTAG+AGG 0.551430 4.2:+54149503 None:intergenic
AATCGTTATGATACGCCACT+TGG 0.553086 4.2:-54150697 MS.gene027456:CDS
GAAGATTTCTTCCTTTGCGT+GGG 0.554338 4.2:-54150608 MS.gene027456:CDS
ATACGACTTTGATCAGCAGT+TGG 0.558928 4.2:+54151048 None:intergenic
TTAATGGCTTCCTTAAGTGC+GGG 0.567307 4.2:+54150426 None:intergenic
AGGGAATTTGTTTGTCATCG+TGG 0.571012 4.2:-54151279 MS.gene027456:CDS
TAATTGTGGTGTAAAGATGT+TGG 0.572351 4.2:-54151190 MS.gene027456:CDS
TTTAATGGCTTCCTTAAGTG+CGG 0.575636 4.2:+54150425 None:intergenic
TAGGAATCAAGCTGGAGAAG+AGG 0.579829 4.2:-54149928 MS.gene027456:CDS
TTGTTACAAAATCCAAAGGT+CGG 0.582656 4.2:-54149308 MS.gene027456:CDS
CGGATGATTTCGAAAGAGAG+TGG 0.582752 4.2:-54150263 MS.gene027456:CDS
ATCATACAAAAGTTGACGGT+GGG 0.583166 4.2:-54149734 MS.gene027456:intron
ACAACGCAACATTAACGAAG+TGG 0.585119 4.2:-54151244 MS.gene027456:CDS
CCCATTGTTACAAAATCCAA+AGG 0.587534 4.2:-54149312 MS.gene027456:CDS
AAATCTTCAAAATGTAATTG+TGG 0.587616 4.2:-54151204 MS.gene027456:CDS
AAAGAAAATGATGCTTCTGA+TGG 0.590975 4.2:-54150886 MS.gene027456:CDS
CAAATTGGAGCATATTATGT+GGG 0.593912 4.2:-54150791 MS.gene027456:CDS
ACGCCACTTGGAATATGGGC+TGG 0.598075 4.2:-54150685 MS.gene027456:CDS
TGACTACATGGCTAGATTTG+GGG 0.607240 4.2:+54149541 None:intergenic
TGCACAATTTGATTTGTGCA+AGG 0.611924 4.2:-54150229 MS.gene027456:CDS
TACCTTTAAAGCCCACGCAA+AGG 0.613106 4.2:+54150597 None:intergenic
GGTGTATCATTTAGAATGTG+CGG 0.618416 4.2:-54150283 MS.gene027456:CDS
TGGGCTGGTGTTGATAATCA+CGG 0.633751 4.2:-54150670 MS.gene027456:CDS
GAATTTGTTTGTCATCGTGG+TGG 0.635704 4.2:-54151276 MS.gene027456:CDS
TACTTATTAGGAGATTGAGA+AGG 0.635742 4.2:+54149153 None:intergenic
AAATCCAAAGGTCGGCCAAG+AGG 0.639624 4.2:-54149300 MS.gene027456:CDS
GCAGCAACTAAAATTGACAA+TGG 0.640124 4.2:-54149774 MS.gene027456:intron
AGAGAACGAGTTGCTACTAG+AGG 0.645909 4.2:-54149441 MS.gene027456:CDS
AGAGGATCAAGACCAAAAGG+AGG 0.673931 4.2:-54149282 MS.gene027456:CDS
TGTCATGTTCCAAGTTGTGG+TGG 0.684261 4.2:-54149231 MS.gene027456:CDS
GTTTAAACAAGAGTTTCACA+AGG 0.684698 4.2:-54150304 MS.gene027456:CDS
ACACCAGCCCATATTCCAAG+TGG 0.684710 4.2:+54150682 None:intergenic
CATCATACAAAAGTTGACGG+TGG 0.688149 4.2:-54149735 MS.gene027456:intron
ATAACGATTTATTCTATACG+TGG 0.695494 4.2:+54150711 None:intergenic
CTTGGAACATGACAGCGACG+AGG 0.719993 4.2:+54149240 None:intergenic
TTGCTGGGATGACAACAACA+GGG 0.722836 4.2:-54150137 MS.gene027456:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCATTATCTTATAATTGA+GGG - chr4.2:54150405-54150424 MS.gene027456:CDS 15.0%
!! TAATTATTATATCTTTGTGT+AGG - chr4.2:54150429-54150448 MS.gene027456:CDS 15.0%
!! AAAGTCATTCAAGATATAAA+AGG - chr4.2:54150988-54151007 MS.gene027456:CDS 20.0%
!! AAATCTTCAAAATGTAATTG+TGG - chr4.2:54149195-54149214 MS.gene027456:CDS 20.0%
!! AATAGTGAATTCATATTTGA+AGG + chr4.2:54149577-54149596 None:intergenic 20.0%
!! GAATCATTATCTTATAATTG+AGG - chr4.2:54150404-54150423 MS.gene027456:CDS 20.0%
!! GCATATATAAAAAGATTCTT+AGG - chr4.2:54150303-54150322 MS.gene027456:CDS 20.0%
!! TATTGATGAAAGTAACAAAT+TGG - chr4.2:54149593-54149612 MS.gene027456:CDS 20.0%
!! TGAATATCTTTATCTAGAAA+AGG + chr4.2:54149462-54149481 None:intergenic 20.0%
!! TTAGAGAAAAAAATAGTTAC+AGG - chr4.2:54149429-54149448 MS.gene027456:CDS 20.0%
!!! TCAATTTTTTCCTTTTTGTT+TGG + chr4.2:54151219-54151238 None:intergenic 20.0%
! AAAATCTGTCAAACTTGTAT+TGG + chr4.2:54150331-54150350 None:intergenic 25.0%
! AATCTCCTAATAAGTAAGTT+TGG - chr4.2:54151253-54151272 MS.gene027456:CDS 25.0%
! ATAACGATTTATTCTATACG+TGG + chr4.2:54149691-54149710 None:intergenic 25.0%
! TAACATCATACAAAAGTTGA+CGG - chr4.2:54150661-54150680 MS.gene027456:CDS 25.0%
! TACTTTGATAGGATCATAAA+AGG - chr4.2:54151347-54151366 MS.gene027456:CDS 25.0%
! TATTATATCTTTGTGTAGGT+TGG - chr4.2:54150433-54150452 MS.gene027456:CDS 25.0%
! TCAAGAATATGTGATTGATT+TGG - chr4.2:54151023-54151042 MS.gene027456:CDS 25.0%
! TTGGTTGCTATAAAAACAAT+TGG - chr4.2:54149228-54149247 MS.gene027456:CDS 25.0%
!! AAATTCCCTTTTATAAAGAC+CGG + chr4.2:54149109-54149128 None:intergenic 25.0%
!! CTTTTCCAATCTTGAATTTT+GGG - chr4.2:54150212-54150231 MS.gene027456:CDS 25.0%
!! TCTTTTCCAATCTTGAATTT+TGG - chr4.2:54150211-54150230 MS.gene027456:CDS 25.0%
!! TGATCTGTAAGAATAGTTTT+TGG + chr4.2:54149949-54149968 None:intergenic 25.0%
!!! TAGTTTTTGGATATTTTCCT+TGG + chr4.2:54149936-54149955 None:intergenic 25.0%
!!! TATAAAAACAATTGGCTTTG+AGG - chr4.2:54149236-54149255 MS.gene027456:CDS 25.0%
!!! TGTAAAGAATTTGAGTTTTC+CGG - chr4.2:54150733-54150752 MS.gene027456:CDS 25.0%
AAAGAAAATGATGCTTCTGA+TGG - chr4.2:54149513-54149532 MS.gene027456:CDS 30.0%
ACAAATTGGAGCATATTATG+TGG - chr4.2:54149607-54149626 MS.gene027456:CDS 30.0%
ACATCACAAACATCGTTATT+TGG + chr4.2:54151060-54151079 None:intergenic 30.0%
ACGAAATTGTCCAAACAAAA+AGG - chr4.2:54151206-54151225 MS.gene027456:CDS 30.0%
ACTTAAGGAAGCCATTAAAA+TGG - chr4.2:54149978-54149997 MS.gene027456:intron 30.0%
ATCACATATTCTTGAGTTTC+TGG + chr4.2:54151018-54151037 None:intergenic 30.0%
CAAATTGGAGCATATTATGT+GGG - chr4.2:54149608-54149627 MS.gene027456:CDS 30.0%
GCAATCCAAACTTACTTATT+AGG + chr4.2:54151261-54151280 None:intergenic 30.0%
GTTTAAACAAGAGTTTCACA+AGG - chr4.2:54150095-54150114 MS.gene027456:CDS 30.0%
TAATGCCCAAAATTCAAGAT+TGG + chr4.2:54150220-54150239 None:intergenic 30.0%
TAATTGTGGTGTAAAGATGT+TGG - chr4.2:54149209-54149228 MS.gene027456:CDS 30.0%
TACTTATTAGGAGATTGAGA+AGG + chr4.2:54151249-54151268 None:intergenic 30.0%
TAGAGATTGAAACTTAGTAG+AGG + chr4.2:54150899-54150918 None:intergenic 30.0%
TAGATAACTCAATCTCATGT+AGG + chr4.2:54150597-54150616 None:intergenic 30.0%
TCAATCTCTAATTCGATGTT+TGG - chr4.2:54150909-54150928 MS.gene027456:CDS 30.0%
TCATCACAATCATGAGTTAT+TGG - chr4.2:54149287-54149306 MS.gene027456:CDS 30.0%
TGAGTTATTGGATGAAAAAG+AGG - chr4.2:54149299-54149318 MS.gene027456:CDS 30.0%
TTCTTTACCAATACAACTCT+CGG + chr4.2:54149499-54149518 None:intergenic 30.0%
TTGATAGGATCATAAAAGGT+TGG - chr4.2:54151351-54151370 MS.gene027456:CDS 30.0%
TTGTTACAAAATCCAAAGGT+CGG - chr4.2:54151091-54151110 MS.gene027456:CDS 30.0%
TTTCCTTCTTGTACTTTGAT+AGG - chr4.2:54151336-54151355 MS.gene027456:CDS 30.0%
! ACCTTTGGATTTTGTAACAA+TGG + chr4.2:54151091-54151110 None:intergenic 30.0%
! ATTTTGCATATGGCATATTG+TGG - chr4.2:54150023-54150042 MS.gene027456:intron 30.0%
! CCTTTGGATTTTGTAACAAT+GGG + chr4.2:54151090-54151109 None:intergenic 30.0%
! GTTTGGCAATTCCATTTTAA+TGG + chr4.2:54149992-54150011 None:intergenic 30.0%
!! AATTGGCTTTGAGGAAAAAT+GGG - chr4.2:54149245-54149264 MS.gene027456:CDS 30.0%
!! GAAAAGGGAGATATTTTGAA+GGG + chr4.2:54150813-54150832 None:intergenic 30.0%
!! TTGGTGTTGTTGTCAAAATT+AGG - chr4.2:54150452-54150471 MS.gene027456:CDS 30.0%
!!! AAGTTGGTTTTCTTTTGCAT+TGG - chr4.2:54150056-54150075 MS.gene027456:intron 30.0%
!!! AGTTGGTTTTCTTTTGCATT+GGG - chr4.2:54150057-54150076 MS.gene027456:intron 30.0%
AGCATGTCTGCATAATATTC+CGG + chr4.2:54150755-54150774 None:intergenic 35.0%
ATCATACAAAAGTTGACGGT+GGG - chr4.2:54150665-54150684 MS.gene027456:CDS 35.0%
ATTCAATCCGAGAGTTGTAT+TGG - chr4.2:54149489-54149508 MS.gene027456:CDS 35.0%
CAAAATGCATGCTTTGTGTT+TGG + chr4.2:54150009-54150028 None:intergenic 35.0%
CCCATTGTTACAAAATCCAA+AGG - chr4.2:54151087-54151106 MS.gene027456:CDS 35.0%
GATCCTATCAAAGTACAAGA+AGG + chr4.2:54151342-54151361 None:intergenic 35.0%
GCAGCAACTAAAATTGACAA+TGG - chr4.2:54150625-54150644 MS.gene027456:CDS 35.0%
GGAAAACAAGAAGTCAATAC+TGG + chr4.2:54151321-54151340 None:intergenic 35.0%
GGAAAACATGTTCTGATATG+AGG + chr4.2:54150523-54150542 None:intergenic 35.0%
GTCAAAATTAGGAATCAAGC+TGG - chr4.2:54150463-54150482 MS.gene027456:CDS 35.0%
TGGAAAAGAGCAAGTCAATA+TGG + chr4.2:54150200-54150219 None:intergenic 35.0%
TTGACTACATGGCTAGATTT+GGG + chr4.2:54150862-54150881 None:intergenic 35.0%
TTTAATGGCTTCCTTAAGTG+CGG + chr4.2:54149977-54149996 None:intergenic 35.0%
TTTCTTGTCTCCATCGAAAA+GGG + chr4.2:54150828-54150847 None:intergenic 35.0%
! ATTCGAGCATACGAATCTTT+TGG - chr4.2:54149645-54149664 MS.gene027456:CDS 35.0%
! ATTTTCCTTGGACAAAAGCT+TGG + chr4.2:54149924-54149943 None:intergenic 35.0%
! CAAAATATCTCCCTTTTCGA+TGG - chr4.2:54150815-54150834 MS.gene027456:CDS 35.0%
! CAAAGCATGCATTTTGCATA+TGG - chr4.2:54150013-54150032 MS.gene027456:intron 35.0%
! CACGGAAATTCGATTTTCTT+TGG - chr4.2:54149747-54149766 MS.gene027456:intron 35.0%
! CAGAACATGTTTTCCTATTG+AGG - chr4.2:54150528-54150547 MS.gene027456:intron 35.0%
! GGTGTATCATTTAGAATGTG+CGG - chr4.2:54150116-54150135 MS.gene027456:CDS 35.0%
! TTGGGCATTAGCTTATTTGA+AGG - chr4.2:54150230-54150249 MS.gene027456:CDS 35.0%
!! CAATTGGCTTTGAGGAAAAA+TGG - chr4.2:54149244-54149263 MS.gene027456:CDS 35.0%
!! CGAAAAGGGAGATATTTTGA+AGG + chr4.2:54150814-54150833 None:intergenic 35.0%
!! TGAAGGACTTTTTCTTTGCT+GGG - chr4.2:54150247-54150266 MS.gene027456:CDS 35.0%
!! TGCACAATTTGATTTGTGCA+AGG - chr4.2:54150170-54150189 MS.gene027456:CDS 35.0%
!! TTGAAGGACTTTTTCTTTGC+TGG - chr4.2:54150246-54150265 MS.gene027456:CDS 35.0%
AACCAGCTTTCGACAACAAT+AGG + chr4.2:54149377-54149396 None:intergenic 40.0%
AATCGTTATGATACGCCACT+TGG - chr4.2:54149702-54149721 MS.gene027456:CDS 40.0%
ACAACGCAACATTAACGAAG+TGG - chr4.2:54149155-54149174 MS.gene027456:intron 40.0%
ACATGCTATTCGTGTGCTAA+TGG - chr4.2:54150768-54150787 MS.gene027456:CDS 40.0%
ACCCATCCGGTCTTTATAAA+AGG - chr4.2:54149100-54149119 MS.gene027456:intron 40.0%
AGAAGATTTCTTCCTTTGCG+TGG - chr4.2:54149790-54149809 MS.gene027456:CDS 40.0%
ATACGACTTTGATCAGCAGT+TGG + chr4.2:54149354-54149373 None:intergenic 40.0%
ATCCTATTGTTGTCGAAAGC+TGG - chr4.2:54149372-54149391 MS.gene027456:CDS 40.0%
ATGATACGCCACTTGGAATA+TGG - chr4.2:54149709-54149728 MS.gene027456:CDS 40.0%
CAACATTGCAGTTGACTACA+TGG + chr4.2:54150873-54150892 None:intergenic 40.0%
CATCATACAAAAGTTGACGG+TGG - chr4.2:54150664-54150683 MS.gene027456:CDS 40.0%
CCCATCCGGTCTTTATAAAA+GGG - chr4.2:54149101-54149120 MS.gene027456:intron 40.0%
CGAAAGAGAGTGGAAATCAA+TGG - chr4.2:54150146-54150165 MS.gene027456:CDS 40.0%
CTCATGTAGGAGCAACTTAA+AGG + chr4.2:54150584-54150603 None:intergenic 40.0%
CTTTCTTGTCTCCATCGAAA+AGG + chr4.2:54150829-54150848 None:intergenic 40.0%
GAAGATTTCTTCCTTTGCGT+GGG - chr4.2:54149791-54149810 MS.gene027456:CDS 40.0%
GAGCATATTATGTGGGCATT+TGG - chr4.2:54149615-54149634 MS.gene027456:CDS 40.0%
GAGTCTAAAAAGAACCCATC+CGG - chr4.2:54149087-54149106 MS.gene027456:intron 40.0%
GTTGACTACATGGCTAGATT+TGG + chr4.2:54150863-54150882 None:intergenic 40.0%
TGACTACATGGCTAGATTTG+GGG + chr4.2:54150861-54150880 None:intergenic 40.0%
TGATACGCCACTTGGAATAT+GGG - chr4.2:54149710-54149729 MS.gene027456:CDS 40.0%
TTAATGGCTTCCTTAAGTGC+GGG + chr4.2:54149976-54149995 None:intergenic 40.0%
TTGTGGCAAAGTTGTCAAGT+TGG - chr4.2:54150040-54150059 MS.gene027456:intron 40.0%
! AACTTCCAAGCTTTTGTCCA+AGG - chr4.2:54149916-54149935 MS.gene027456:CDS 40.0%
! AGGAGCAACTTAAAGGCATA+TGG + chr4.2:54150577-54150596 None:intergenic 40.0%
! AGGGAATTTGTTTGTCATCG+TGG - chr4.2:54149120-54149139 MS.gene027456:intron 40.0%
! CCCTTTTATAAAGACCGGAT+GGG + chr4.2:54149104-54149123 None:intergenic 40.0%
! GAATTTGTTTGTCATCGTGG+TGG - chr4.2:54149123-54149142 MS.gene027456:intron 40.0%
! TCCCTTTTATAAAGACCGGA+TGG + chr4.2:54149105-54149124 None:intergenic 40.0%
! TGATTAGTTCCACCACAACT+TGG + chr4.2:54151180-54151199 None:intergenic 40.0%
!! AAGGGAGATATTTTGAAGGG+AGG + chr4.2:54150810-54150829 None:intergenic 40.0%
AGAGAACGAGTTGCTACTAG+AGG - chr4.2:54150958-54150977 MS.gene027456:CDS 45.0%
AGAGGATCAAGACCAAAAGG+AGG - chr4.2:54151117-54151136 MS.gene027456:CDS 45.0%
AGATCAAGATCCCGCACTTA+AGG - chr4.2:54149963-54149982 MS.gene027456:intron 45.0%
CCAAGAGGATCAAGACCAAA+AGG - chr4.2:54151114-54151133 MS.gene027456:CDS 45.0%
CGGATGATTTCGAAAGAGAG+TGG - chr4.2:54150136-54150155 MS.gene027456:CDS 45.0%
GAGGAGTTGAAGCTGCAAAT+AGG - chr4.2:54151136-54151155 MS.gene027456:CDS 45.0%
GATGCAGCATGTTCCTCAAT+AGG + chr4.2:54150544-54150563 None:intergenic 45.0%
GTTGGAATGTTGCGATAAGC+AGG + chr4.2:54149336-54149355 None:intergenic 45.0%
TAGGAATCAAGCTGGAGAAG+AGG - chr4.2:54150471-54150490 MS.gene027456:CDS 45.0%
TGTCATGTTCCAAGTTGTGG+TGG - chr4.2:54151168-54151187 MS.gene027456:CDS 45.0%
TTGCTGGGATGACAACAACA+GGG - chr4.2:54150262-54150281 MS.gene027456:CDS 45.0%
TTTGCTGGGATGACAACAAC+AGG - chr4.2:54150261-54150280 MS.gene027456:CDS 45.0%
! TTCCTTTGCGTGGGCTTTAA+AGG - chr4.2:54149800-54149819 MS.gene027456:CDS 45.0%
!! TACCTTTAAAGCCCACGCAA+AGG + chr4.2:54149805-54149824 None:intergenic 45.0%
!! TGGCTTTGAGGAAAAATGGG+TGG - chr4.2:54149248-54149267 MS.gene027456:CDS 45.0%
!! TGGGCTGGTGTTGATAATCA+CGG - chr4.2:54149729-54149748 MS.gene027456:intron 45.0%
!!! CCTTTTGGTCTTGATCCTCT+TGG + chr4.2:54151117-54151136 None:intergenic 45.0%
AAATCCAAAGGTCGGCCAAG+AGG - chr4.2:54151099-54151118 MS.gene027456:CDS 50.0%
ACACCAGCCCATATTCCAAG+TGG + chr4.2:54149720-54149739 None:intergenic 50.0%
CGCTGTCATGTTCCAAGTTG+TGG - chr4.2:54151165-54151184 MS.gene027456:CDS 50.0%
! GCAGCTTCAACTCCTCCTTT+TGG + chr4.2:54151132-54151151 None:intergenic 50.0%
ACGCCACTTGGAATATGGGC+TGG - chr4.2:54149714-54149733 MS.gene027456:CDS 55.0%
ACGGTGGGCGTCAAGTAAGT+TGG - chr4.2:54150680-54150699 MS.gene027456:CDS 55.0%
CTTGGAACATGACAGCGACG+AGG + chr4.2:54151162-54151181 None:intergenic 55.0%
TGATCCTCTTGGCCGACCTT+TGG + chr4.2:54151106-54151125 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 54149042 54151379 54149042 ID=MS.gene027456
chr4.2 mRNA 54149042 54151379 54149042 ID=MS.gene027456.t1;Parent=MS.gene027456
chr4.2 exon 54150600 54151379 54150600 ID=MS.gene027456.t1.exon1;Parent=MS.gene027456.t1
chr4.2 CDS 54150600 54151379 54150600 ID=cds.MS.gene027456.t1;Parent=MS.gene027456.t1
chr4.2 exon 54150062 54150505 54150062 ID=MS.gene027456.t1.exon2;Parent=MS.gene027456.t1
chr4.2 CDS 54150062 54150505 54150062 ID=cds.MS.gene027456.t1;Parent=MS.gene027456.t1
chr4.2 exon 54149775 54149970 54149775 ID=MS.gene027456.t1.exon3;Parent=MS.gene027456.t1
chr4.2 CDS 54149775 54149970 54149775 ID=cds.MS.gene027456.t1;Parent=MS.gene027456.t1
chr4.2 exon 54149156 54149744 54149156 ID=MS.gene027456.t1.exon4;Parent=MS.gene027456.t1
chr4.2 CDS 54149156 54149744 54149156 ID=cds.MS.gene027456.t1;Parent=MS.gene027456.t1
chr4.2 exon 54149042 54149063 54149042 ID=MS.gene027456.t1.exon5;Parent=MS.gene027456.t1
chr4.2 CDS 54149042 54149063 54149042 ID=cds.MS.gene027456.t1;Parent=MS.gene027456.t1
Gene Sequence

>MS.gene027456

ATGGATTTTCGATCAGGCGCGAACACATTCAAATCTTGTACAAAAGAGTCTAAAAAGAACCCATCCGGTCTTTATAAAAGGGAATTTGTTTGTCATCGTGGTGGTATTGTTAAACAACGCAACATTAACGAAGTGGAATGTCAAAGAAAACGAAAATCTTCAAAATGTAATTGTGGTGTAAAGATGTTGGTTGCTATAAAAACAATTGGCTTTGAGGAAAAATGGGTGGTTACATATTTAAACAATCATCACAATCATGAGTTATTGGATGAAAAAGAGGTTCAATTTCTTCCTGCTTATCGCAACATTCCAACTGCTGATCAAAGTCGTATCCTATTGTTGTCGAAAGCTGGTTGCTCTATAAGTATTATAATGAGAGTGCTTGAGTTAGAGAAAAAAATAGTTACAGGCAATCTACCTTTTCTAGATAAAGATATTCAAAATTTTATTCAATCCGAGAGTTGTATTGGTAAAGAAAATGATGCTTCTGATGGTCTTAAATTATGCAAAAATTTGAAAGATATTGATGATGCCTTCAAATATGAATTCACTATTGATGAAAGTAACAAATTGGAGCATATTATGTGGGCATTTGGTGATTCAATTCGAGCATACGAATCTTTTGGAGATGTTGTAGTTTTTGATACCACGTATAGAATAAATCGTTATGATACGCCACTTGGAATATGGGCTGGTGTTGATAATCACGGAAATTCGATTTTCTTTGGTTGTGTTCTATTGAAGAATGAGAAGATTTCTTCCTTTGCGTGGGCTTTAAAGGTAAATTTTTGTTTACTTAAAGTTGTTATTGTTGTTGAATTTTTAACTTTGAAGCTTTGTTGAAGTCTTTTATATTTATGTTATTATTTTTCAGAACTTCCAAGCTTTTGTCCAAGGAAAATATCCAAAAACTATTCTTACAGATCAAGATCCCGCACTTAAGGAAGCCATTAAAATGGAATTGCCAAACACAAAGCATGCATTTTGCATATGGCATATTGTGGCAAAGTTGTCAAGTTGGTTTTCTTTTGCATTGGGTTCAAGATATAATGAGTTTAAACAAGAGTTTCACAAGGTGTATCATTTAGAATGTGCGGATGATTTCGAAAGAGAGTGGAAATCAATGGTTGCACAATTTGATTTGTGCAAGGATAACCATATTGACTTGCTCTTTTCCAATCTTGAATTTTGGGCATTAGCTTATTTGAAGGACTTTTTCTTTGCTGGGATGACAACAACAGGGCGCTCAGAATCGATAAATGCATATATAAAAAGATTCTTAGGCGCCAATACAAGTTTGACAGATTTTGTAAATCAAGTATGAACTTACTTGAGCTACTTGTTAAAAGTACATTAGTTTTTGAATCATTATCTTATAATTGAGGGATAATTATTATATCTTTGTGTAGGTTGGTGTTGTTGTCAAAATTAGGAATCAAGCTGGAGAAGAGGCAAGAATGCGTCAAAAGTATCACAATCCTCATATCAGAACATGTTTTCCTATTGAGGAACATGCTGCATCTGTACTCACGCCATATGCCTTTAAGTTGCTCCTACATGAGATTGAGTTATCTACAAAATATGCAGCAACTAAAATTGACAATGGTTCTTATATTGTGTAACATCATACAAAAGTTGACGGTGGGCGTCAAGTAAGTTGGATAGTAGAAAAAGAGTCAATTCATTGCTCTTGTAAAGAATTTGAGTTTTCCGGAATATTATGCAGACATGCTATTCGTGTGCTAATGGTGAAAAATTATTTTAGCCTCCCTTCAAAATATCTCCCTTTTCGATGGAGACAAGAAAGTTCATTGATCCCCAAATCTAGCCATGTAGTCAACTGCAATGTTGCTTCCTCTACTAAGTTTCAATCTCTAATTCGATGTTTGGAAGTTGAATCATTAAAGACAAGCGAAAGAGAACGAGTTGCTACTAGAGGATTAGAAAAAGTCATTCAAGATATAAAAGGCATGCCAGAAACTCAAGAATATGTGATTGATTTGGAACATGATGCTCCAAATAACGATGTTTGTGATGTTGAAAATCCCATTGTTACAAAATCCAAAGGTCGGCCAAGAGGATCAAGACCAAAAGGAGGAGTTGAAGCTGCAAATAGGCCTCGTCGCTGTCATGTTCCAAGTTGTGGTGGAACTAATCATGACTCACGAAATTGTCCAAACAAAAAGGAAAAAATTGAAGTATTGCCTTCTCAATCTCCTAATAAGTAAGTTTGGATTGCTATGTGTTGTTCTTTTCATGTCTACATTTATTGAATGCCAGTATTGACTTCTTGTTTTCCTTCTTGTACTTTGATAGGATCATAAAAGGTTGGAAGTAG
Protein sequence

>MS.gene027456.t1

MDFRSGANTFKSCTKESKKNPSGLYKREFVCHRGGIVKQRNINEVECQRKRKSSKCNCGVKMLVAIKTIGFEEKWVVTYLNNHHNHELLDEKEVQFLPAYRNIPTADQSRILLLSKAGCSISIIMRVLELEKKIVTGNLPFLDKDIQNFIQSESCIGKENDASDGLKLCKNLKDIDDAFKYEFTIDESNKLEHIMWAFGDSIRAYESFGDVVVFDTTYRINRYDTPLGIWAGVDNHGNSIFFGCVLLKNEKISSFAWALKNFQAFVQGKYPKTILTDQDPALKEAIKMELPNTKHAFCIWHIVAKLSSWFSFALGSRYNEFKQEFHKVYHLECADDFEREWKSMVAQFDLCKDNHIDLLFSNLEFWALAYLKDFFFAGMTTTGRSESINAYIKRFLGANTSLTDFVNQVGVVVKIRNQAGEEARMRQKYHNPHIRTCFPIEEHAASVLTPYAFKLLLHEIELSTKYAATKIDNVDGGRQVSWIVEKESIHCSCKEFEFSGILCRHAIRVLMVKNYFSLPSKYLPFRWRQESSLIPKSSHVVNCNVASSTKFQSLIRCLEVESLKTSERERVATRGLEKVIQDIKGMPETQEYVIDLEHDAPNNDVCDVENPIVTKSKGRPRGSRPKGGVEAANRPRRCHVPSCGGTNHDSRNCPNKKEKIEVLPSQSPNKIIKGWK