AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027457

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027457.t1 MTR_3g450850 68.269 208 66 0 9 216 79 286 7.92e-97 302
MS.gene027457.t1 MTR_4g071400 80.992 121 23 0 511 631 1 121 2.77e-63 206
MS.gene027457.t1 MTR_3g012160 96.774 93 3 0 125 217 1 93 6.15e-56 185
MS.gene027457.t1 MTR_4g035820 40.670 209 122 1 26 232 116 324 3.94e-43 166
MS.gene027457.t1 MTR_4g035820 36.129 155 83 2 365 503 326 480 8.93e-21 97.4
MS.gene027457.t1 MTR_6g051425 30.085 236 154 5 27 257 62 291 1.14e-23 103
MS.gene027457.t1 MTR_1g070355 29.665 209 144 3 14 222 104 309 2.01e-21 99.0
MS.gene027457.t1 MTR_3g095940 86.364 44 6 0 430 473 90 133 2.33e-18 82.8
MS.gene027457.t1 MTR_5g083630 65.517 58 20 0 126 183 19 76 1.34e-17 79.3
MS.gene027457.t1 MTR_6g014355 40.708 113 65 1 58 168 14 126 6.38e-16 78.6
MS.gene027457.t1 MTR_5g076070 25.128 195 139 5 24 216 93 282 1.66e-13 72.0
MS.gene027457.t1 MTR_7g446510 31.928 166 107 5 74 236 167 329 4.26e-13 71.2
MS.gene027457.t1 MTR_7g446480 31.928 166 107 5 74 236 152 314 9.14e-13 70.1
MS.gene027457.t1 MTR_8g446020 31.544 149 94 3 84 228 112 256 2.04e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027457.t1 AT1G10240 45.347 591 313 2 23 603 89 679 0.0 553
MS.gene027457.t1 AT1G10240 45.347 591 313 2 23 603 89 679 0.0 553
MS.gene027457.t1 AT5G28530 40.698 602 328 6 4 584 77 670 2.45e-153 459
MS.gene027457.t1 AT5G28530 40.698 602 328 6 4 584 77 670 2.45e-153 459
MS.gene027457.t1 AT5G28530 40.864 602 331 6 4 584 77 674 1.04e-152 458
MS.gene027457.t1 AT5G28530 35.980 592 307 7 4 584 77 607 3.93e-119 369
MS.gene027457.t1 AT5G28530 36.149 592 310 7 4 584 77 611 1.02e-118 368
MS.gene027457.t1 AT4G38180 30.597 536 338 9 23 534 113 638 1.06e-74 256
MS.gene027457.t1 AT4G15090 26.462 650 440 13 10 632 76 714 8.51e-68 238
MS.gene027457.t1 AT4G15090 26.462 650 440 13 10 632 76 714 8.51e-68 238
MS.gene027457.t1 AT4G15090 26.462 650 440 13 10 632 76 714 8.51e-68 238
MS.gene027457.t1 AT4G15090 26.462 650 440 13 10 632 76 714 8.51e-68 238
MS.gene027457.t1 AT4G15090 26.462 650 440 13 10 632 76 714 8.51e-68 238
MS.gene027457.t1 AT1G76320 26.542 535 364 8 6 527 21 539 6.15e-60 213
MS.gene027457.t1 AT1G76320 26.542 535 364 8 6 527 21 539 1.41e-59 213
MS.gene027457.t1 AT1G76320 26.542 535 364 8 6 527 21 539 1.41e-59 213
MS.gene027457.t1 AT1G76320 26.542 535 364 8 6 527 21 539 1.48e-59 213
MS.gene027457.t1 AT3G22170 26.600 500 318 11 50 531 149 617 7.12e-56 204
MS.gene027457.t1 AT3G22170 26.600 500 318 11 50 531 149 617 7.12e-56 204
MS.gene027457.t1 AT2G27110 26.336 524 345 13 63 567 111 612 8.37e-50 187
MS.gene027457.t1 AT2G27110 26.336 524 345 13 63 567 111 612 8.37e-50 187
MS.gene027457.t1 AT2G27110 27.816 435 284 10 152 567 44 467 1.38e-47 179
MS.gene027457.t1 AT3G06250 26.667 525 302 13 27 527 234 699 6.44e-46 175
MS.gene027457.t1 AT3G06250 26.667 525 302 13 27 527 234 699 6.44e-46 175
MS.gene027457.t1 AT3G06250 26.667 525 302 13 27 527 234 699 6.44e-46 175
MS.gene027457.t1 AT2G32250 25.333 375 265 4 172 532 180 553 1.25e-43 168
MS.gene027457.t1 AT2G32250 25.333 375 265 4 172 532 139 512 1.51e-43 168
MS.gene027457.t1 AT2G32250 25.333 375 265 4 172 532 139 512 1.56e-43 168
MS.gene027457.t1 AT2G32250 25.333 375 265 4 172 532 180 553 1.91e-43 168
MS.gene027457.t1 AT2G32250 25.333 375 265 4 172 532 180 553 1.91e-43 168
MS.gene027457.t1 AT2G32250 25.333 375 265 4 172 532 180 553 1.91e-43 168
MS.gene027457.t1 AT2G32250 25.333 375 265 4 172 532 180 553 1.98e-43 168
MS.gene027457.t1 AT2G32250 25.333 375 265 4 172 532 180 553 1.98e-43 168
MS.gene027457.t1 AT4G38170 27.731 357 241 4 193 533 45 400 2.81e-42 161
MS.gene027457.t1 AT4G19990 25.948 501 308 8 51 531 44 501 5.97e-42 162
MS.gene027457.t1 AT4G19990 25.948 501 308 8 51 531 101 558 8.80e-42 162
MS.gene027457.t1 AT4G19990 25.948 501 308 8 51 531 101 558 9.44e-42 162
MS.gene027457.t1 AT4G19990 25.948 501 308 8 51 531 101 558 9.44e-42 162
MS.gene027457.t1 AT4G19990 25.948 501 308 8 51 531 101 558 1.14e-41 162
MS.gene027457.t1 AT4G19990 25.948 501 308 8 51 531 101 558 1.14e-41 162
MS.gene027457.t1 AT4G19990 25.948 501 308 8 51 531 115 572 1.25e-41 162
MS.gene027457.t1 AT5G18960 25.626 519 318 15 27 527 255 723 2.76e-40 158
MS.gene027457.t1 AT5G18960 25.626 519 318 15 27 527 255 723 2.76e-40 158
MS.gene027457.t1 AT1G80010 25.323 387 238 9 179 530 289 659 3.20e-36 145
MS.gene027457.t1 AT1G52520 25.721 416 276 10 142 530 227 636 4.09e-36 145

Find 94 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCACATATTCTTGAGTTTC+TGG 0.087082 4.2:+54160054 None:intergenic
ACATCACAAACATCGTTATT+TGG 0.152623 4.2:+54160012 None:intergenic
ACCCATCCGGTCTTTATAAA+AGG 0.176532 4.2:-54161969 MS.gene027457:CDS
GCAATCCAAACTTACTTATT+AGG 0.216308 4.2:+54159811 None:intergenic
ATGATACGCCACTTGGAATA+TGG 0.219407 4.2:-54161360 MS.gene027457:CDS
TTCCTTTGCGTGGGCTTTAA+AGG 0.228847 4.2:-54161269 MS.gene027457:intron
TACTTTGATAGGATCATAAA+AGG 0.274738 4.2:-54159722 MS.gene027457:intron
ATTCGAGCATACGAATCTTT+TGG 0.279666 4.2:-54161424 MS.gene027457:CDS
CTCATGTAGGAGCAACTTAA+AGG 0.285515 4.2:+54160488 None:intergenic
TCATCACAATCATGAGTTAT+TGG 0.289585 4.2:-54161782 MS.gene027457:CDS
ACGAAATTGTCCAAACAAAA+AGG 0.298732 4.2:-54159863 MS.gene027457:CDS
GTTGACTACATGGCTAGATT+TGG 0.300626 4.2:+54160209 None:intergenic
TTGACTACATGGCTAGATTT+GGG 0.326040 4.2:+54160210 None:intergenic
GCAGCTTCAACTCCTCCTTT+TGG 0.329186 4.2:+54159940 None:intergenic
TCAAGAATATGTGATTGATT+TGG 0.332734 4.2:-54160046 MS.gene027457:CDS
TGATCCTCTTGGCCGACCTT+TGG 0.339991 4.2:+54159966 None:intergenic
TTGGTGTTGTTGTCAAAATT+AGG 0.343446 4.2:-54160617 MS.gene027457:CDS
TATTGATGAAAGTAACAAAT+TGG 0.343540 4.2:-54161476 MS.gene027457:CDS
TCAATCTCTAATTCGATGTT+TGG 0.356770 4.2:-54160160 MS.gene027457:CDS
ACTTAAGGAAGCCATTAAAA+TGG 0.358796 4.2:-54161091 MS.gene027457:CDS
AAAGTCATTCAAGATATAAA+AGG 0.358979 4.2:-54160081 MS.gene027457:CDS
AAAATCTGTCAAACTTGTAT+TGG 0.376571 4.2:+54160741 None:intergenic
AGCATGTCTGCATAATATTC+CGG 0.383529 4.2:+54160317 None:intergenic
CAAAATGCATGCTTTGTGTT+TGG 0.392144 4.2:+54161063 None:intergenic
AGATCAAGATCCCGCACTTA+AGG 0.392664 4.2:-54161106 MS.gene027457:CDS
GAGGAGTTGAAGCTGCAAAT+AGG 0.394021 4.2:-54159933 MS.gene027457:CDS
AATAGTGAATTCATATTTGA+AGG 0.396961 4.2:+54161495 None:intergenic
GATGCAGCATGTTCCTCAAT+AGG 0.398909 4.2:+54160528 None:intergenic
TGGAAAAGAGCAAGTCAATA+TGG 0.408931 4.2:+54160872 None:intergenic
TTTGCTGGGATGACAACAAC+AGG 0.410409 4.2:-54160808 MS.gene027457:CDS
GAGCATATTATGTGGGCATT+TGG 0.419517 4.2:-54161454 MS.gene027457:CDS
ATTCAATCCGAGAGTTGTAT+TGG 0.424169 4.2:-54161580 MS.gene027457:CDS
AACCAGCTTTCGACAACAAT+AGG 0.437905 4.2:+54161695 None:intergenic
CTTTCTTGTCTCCATCGAAA+AGG 0.441125 4.2:+54160243 None:intergenic
TTCTTTACCAATACAACTCT+CGG 0.441999 4.2:+54161573 None:intergenic
GGAAAACATGTTCTGATATG+AGG 0.442250 4.2:+54160549 None:intergenic
TGATACGCCACTTGGAATAT+GGG 0.449284 4.2:-54161359 MS.gene027457:CDS
TTGGGCATTAGCTTATTTGA+AGG 0.451954 4.2:-54160839 MS.gene027457:CDS
CCCATCCGGTCTTTATAAAA+GGG 0.452968 4.2:-54161968 MS.gene027457:CDS
ATCCTATTGTTGTCGAAAGC+TGG 0.455314 4.2:-54161697 MS.gene027457:CDS
ACATGCTATTCGTGTGCTAA+TGG 0.455837 4.2:-54160301 MS.gene027457:CDS
TGAATATCTTTATCTAGAAA+AGG 0.460825 4.2:+54161610 None:intergenic
CGAAAGAGAGTGGAAATCAA+TGG 0.466880 4.2:-54160923 MS.gene027457:CDS
TTGATAGGATCATAAAAGGT+TGG 0.470332 4.2:-54159718 MS.gene027457:intron
CAACATTGCAGTTGACTACA+TGG 0.482092 4.2:+54160199 None:intergenic
TTTCTTGTCTCCATCGAAAA+GGG 0.485154 4.2:+54160244 None:intergenic
GTTGGAATGTTGCGATAAGC+AGG 0.492886 4.2:+54161736 None:intergenic
TGATTAGTTCCACCACAACT+TGG 0.507884 4.2:+54159892 None:intergenic
CGCTGTCATGTTCCAAGTTG+TGG 0.511566 4.2:-54159904 MS.gene027457:CDS
TAATGCCCAAAATTCAAGAT+TGG 0.511972 4.2:+54160852 None:intergenic
CCAAGAGGATCAAGACCAAA+AGG 0.514192 4.2:-54159955 MS.gene027457:CDS
AGGAGCAACTTAAAGGCATA+TGG 0.516663 4.2:+54160495 None:intergenic
GTCAAAATTAGGAATCAAGC+TGG 0.523988 4.2:-54160606 MS.gene027457:CDS
ACGGTGGGCGTCAAGTAAGT+TGG 0.528714 4.2:-54160389 MS.gene027457:CDS
TTGTGGCAAAGTTGTCAAGT+TGG 0.535071 4.2:-54161029 MS.gene027457:CDS
ACAAATTGGAGCATATTATG+TGG 0.542960 4.2:-54161462 MS.gene027457:CDS
TAGATAACTCAATCTCATGT+AGG 0.545292 4.2:+54160475 None:intergenic
AGAAGATTTCTTCCTTTGCG+TGG 0.551128 4.2:-54161279 MS.gene027457:CDS
TAGAGATTGAAACTTAGTAG+AGG 0.551430 4.2:+54160173 None:intergenic
AATCGTTATGATACGCCACT+TGG 0.553086 4.2:-54161367 MS.gene027457:CDS
GAAGATTTCTTCCTTTGCGT+GGG 0.554338 4.2:-54161278 MS.gene027457:CDS
ATACGACTTTGATCAGCAGT+TGG 0.558928 4.2:+54161718 None:intergenic
TTAATGGCTTCCTTAAGTGC+GGG 0.567307 4.2:+54161096 None:intergenic
AGGGAATTTGTTTGTCATCG+TGG 0.571012 4.2:-54161949 MS.gene027457:CDS
TAATTGTGGTGTAAAGATGT+TGG 0.572351 4.2:-54161860 MS.gene027457:CDS
TTTAATGGCTTCCTTAAGTG+CGG 0.575636 4.2:+54161095 None:intergenic
TAGGAATCAAGCTGGAGAAG+AGG 0.579829 4.2:-54160598 MS.gene027457:CDS
TTGTTACAAAATCCAAAGGT+CGG 0.582656 4.2:-54159978 MS.gene027457:CDS
CGGATGATTTCGAAAGAGAG+TGG 0.582752 4.2:-54160933 MS.gene027457:CDS
ATCATACAAAAGTTGACGGT+GGG 0.583166 4.2:-54160404 MS.gene027457:intron
ACAACGCAACATTAACGAAG+TGG 0.585119 4.2:-54161914 MS.gene027457:CDS
CCCATTGTTACAAAATCCAA+AGG 0.587534 4.2:-54159982 MS.gene027457:CDS
AAATCTTCAAAATGTAATTG+TGG 0.587616 4.2:-54161874 MS.gene027457:CDS
AAAGAAAATGATGCTTCTGA+TGG 0.590975 4.2:-54161556 MS.gene027457:CDS
CAAATTGGAGCATATTATGT+GGG 0.593912 4.2:-54161461 MS.gene027457:CDS
ACGCCACTTGGAATATGGGC+TGG 0.598075 4.2:-54161355 MS.gene027457:CDS
TGACTACATGGCTAGATTTG+GGG 0.607240 4.2:+54160211 None:intergenic
TGCACAATTTGATTTGTGCA+AGG 0.611924 4.2:-54160899 MS.gene027457:CDS
TACCTTTAAAGCCCACGCAA+AGG 0.613106 4.2:+54161267 None:intergenic
GGTGTATCATTTAGAATGTG+CGG 0.618416 4.2:-54160953 MS.gene027457:CDS
TGGGCTGGTGTTGATAATCA+CGG 0.633751 4.2:-54161340 MS.gene027457:CDS
GAATTTGTTTGTCATCGTGG+TGG 0.635704 4.2:-54161946 MS.gene027457:CDS
TACTTATTAGGAGATTGAGA+AGG 0.635742 4.2:+54159823 None:intergenic
AAATCCAAAGGTCGGCCAAG+AGG 0.639624 4.2:-54159970 MS.gene027457:CDS
GCAGCAACTAAAATTGACAA+TGG 0.640124 4.2:-54160444 MS.gene027457:intron
AGAGAACGAGTTGCTACTAG+AGG 0.645909 4.2:-54160111 MS.gene027457:CDS
AGAGGATCAAGACCAAAAGG+AGG 0.673931 4.2:-54159952 MS.gene027457:CDS
TGTCATGTTCCAAGTTGTGG+TGG 0.684261 4.2:-54159901 MS.gene027457:CDS
GTTTAAACAAGAGTTTCACA+AGG 0.684698 4.2:-54160974 MS.gene027457:CDS
ACACCAGCCCATATTCCAAG+TGG 0.684710 4.2:+54161352 None:intergenic
CATCATACAAAAGTTGACGG+TGG 0.688149 4.2:-54160405 MS.gene027457:intron
ATAACGATTTATTCTATACG+TGG 0.695494 4.2:+54161381 None:intergenic
CTTGGAACATGACAGCGACG+AGG 0.719993 4.2:+54159910 None:intergenic
TTGCTGGGATGACAACAACA+GGG 0.722836 4.2:-54160807 MS.gene027457:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCATTATCTTATAATTGA+GGG - chr4.2:54161075-54161094 MS.gene027457:CDS 15.0%
!! TAATTATTATATCTTTGTGT+AGG - chr4.2:54161099-54161118 MS.gene027457:CDS 15.0%
!! AAAGTCATTCAAGATATAAA+AGG - chr4.2:54161658-54161677 MS.gene027457:CDS 20.0%
!! AAATCTTCAAAATGTAATTG+TGG - chr4.2:54159865-54159884 MS.gene027457:CDS 20.0%
!! AATAGTGAATTCATATTTGA+AGG + chr4.2:54160247-54160266 None:intergenic 20.0%
!! GAATCATTATCTTATAATTG+AGG - chr4.2:54161074-54161093 MS.gene027457:CDS 20.0%
!! GCATATATAAAAAGATTCTT+AGG - chr4.2:54160973-54160992 MS.gene027457:CDS 20.0%
!! TATTGATGAAAGTAACAAAT+TGG - chr4.2:54160263-54160282 MS.gene027457:CDS 20.0%
!! TGAATATCTTTATCTAGAAA+AGG + chr4.2:54160132-54160151 None:intergenic 20.0%
!! TTAGAGAAAAAAATAGTTAC+AGG - chr4.2:54160099-54160118 MS.gene027457:CDS 20.0%
!!! TCAATTTTTTCCTTTTTGTT+TGG + chr4.2:54161889-54161908 None:intergenic 20.0%
! AAAATCTGTCAAACTTGTAT+TGG + chr4.2:54161001-54161020 None:intergenic 25.0%
! AATCTCCTAATAAGTAAGTT+TGG - chr4.2:54161923-54161942 MS.gene027457:CDS 25.0%
! ATAACGATTTATTCTATACG+TGG + chr4.2:54160361-54160380 None:intergenic 25.0%
! TAACATCATACAAAAGTTGA+CGG - chr4.2:54161331-54161350 MS.gene027457:CDS 25.0%
! TACTTTGATAGGATCATAAA+AGG - chr4.2:54162017-54162036 MS.gene027457:CDS 25.0%
! TATTATATCTTTGTGTAGGT+TGG - chr4.2:54161103-54161122 MS.gene027457:CDS 25.0%
! TCAAGAATATGTGATTGATT+TGG - chr4.2:54161693-54161712 MS.gene027457:CDS 25.0%
! TTGGTTGCTATAAAAACAAT+TGG - chr4.2:54159898-54159917 MS.gene027457:CDS 25.0%
!! AAATTCCCTTTTATAAAGAC+CGG + chr4.2:54159779-54159798 None:intergenic 25.0%
!! CTTTTCCAATCTTGAATTTT+GGG - chr4.2:54160882-54160901 MS.gene027457:CDS 25.0%
!! TCTTTTCCAATCTTGAATTT+TGG - chr4.2:54160881-54160900 MS.gene027457:CDS 25.0%
!! TGATCTGTAAGAATAGTTTT+TGG + chr4.2:54160619-54160638 None:intergenic 25.0%
!!! TAGTTTTTGGATATTTTCCT+TGG + chr4.2:54160606-54160625 None:intergenic 25.0%
!!! TATAAAAACAATTGGCTTTG+AGG - chr4.2:54159906-54159925 MS.gene027457:CDS 25.0%
!!! TGTAAAGAATTTGAGTTTTC+CGG - chr4.2:54161403-54161422 MS.gene027457:CDS 25.0%
AAAGAAAATGATGCTTCTGA+TGG - chr4.2:54160183-54160202 MS.gene027457:CDS 30.0%
ACAAATTGGAGCATATTATG+TGG - chr4.2:54160277-54160296 MS.gene027457:CDS 30.0%
ACATCACAAACATCGTTATT+TGG + chr4.2:54161730-54161749 None:intergenic 30.0%
ACGAAATTGTCCAAACAAAA+AGG - chr4.2:54161876-54161895 MS.gene027457:CDS 30.0%
ACTTAAGGAAGCCATTAAAA+TGG - chr4.2:54160648-54160667 MS.gene027457:intron 30.0%
ATCACATATTCTTGAGTTTC+TGG + chr4.2:54161688-54161707 None:intergenic 30.0%
CAAATTGGAGCATATTATGT+GGG - chr4.2:54160278-54160297 MS.gene027457:CDS 30.0%
GCAATCCAAACTTACTTATT+AGG + chr4.2:54161931-54161950 None:intergenic 30.0%
GTTTAAACAAGAGTTTCACA+AGG - chr4.2:54160765-54160784 MS.gene027457:CDS 30.0%
TAATGCCCAAAATTCAAGAT+TGG + chr4.2:54160890-54160909 None:intergenic 30.0%
TAATTGTGGTGTAAAGATGT+TGG - chr4.2:54159879-54159898 MS.gene027457:CDS 30.0%
TACTTATTAGGAGATTGAGA+AGG + chr4.2:54161919-54161938 None:intergenic 30.0%
TAGAGATTGAAACTTAGTAG+AGG + chr4.2:54161569-54161588 None:intergenic 30.0%
TAGATAACTCAATCTCATGT+AGG + chr4.2:54161267-54161286 None:intergenic 30.0%
TCAATCTCTAATTCGATGTT+TGG - chr4.2:54161579-54161598 MS.gene027457:CDS 30.0%
TCATCACAATCATGAGTTAT+TGG - chr4.2:54159957-54159976 MS.gene027457:CDS 30.0%
TGAGTTATTGGATGAAAAAG+AGG - chr4.2:54159969-54159988 MS.gene027457:CDS 30.0%
TTCTTTACCAATACAACTCT+CGG + chr4.2:54160169-54160188 None:intergenic 30.0%
TTGATAGGATCATAAAAGGT+TGG - chr4.2:54162021-54162040 MS.gene027457:CDS 30.0%
TTGTTACAAAATCCAAAGGT+CGG - chr4.2:54161761-54161780 MS.gene027457:CDS 30.0%
TTTCCTTCTTGTACTTTGAT+AGG - chr4.2:54162006-54162025 MS.gene027457:CDS 30.0%
! ACCTTTGGATTTTGTAACAA+TGG + chr4.2:54161761-54161780 None:intergenic 30.0%
! ATTTTGCATATGGCATATTG+TGG - chr4.2:54160693-54160712 MS.gene027457:intron 30.0%
! CCTTTGGATTTTGTAACAAT+GGG + chr4.2:54161760-54161779 None:intergenic 30.0%
! GTTTGGCAATTCCATTTTAA+TGG + chr4.2:54160662-54160681 None:intergenic 30.0%
!! AATTGGCTTTGAGGAAAAAT+GGG - chr4.2:54159915-54159934 MS.gene027457:CDS 30.0%
!! GAAAAGGGAGATATTTTGAA+GGG + chr4.2:54161483-54161502 None:intergenic 30.0%
!! TTGGTGTTGTTGTCAAAATT+AGG - chr4.2:54161122-54161141 MS.gene027457:CDS 30.0%
!!! AAGTTGGTTTTCTTTTGCAT+TGG - chr4.2:54160726-54160745 MS.gene027457:intron 30.0%
!!! AGTTGGTTTTCTTTTGCATT+GGG - chr4.2:54160727-54160746 MS.gene027457:intron 30.0%
AGCATGTCTGCATAATATTC+CGG + chr4.2:54161425-54161444 None:intergenic 35.0%
ATCATACAAAAGTTGACGGT+GGG - chr4.2:54161335-54161354 MS.gene027457:CDS 35.0%
ATTCAATCCGAGAGTTGTAT+TGG - chr4.2:54160159-54160178 MS.gene027457:CDS 35.0%
CAAAATGCATGCTTTGTGTT+TGG + chr4.2:54160679-54160698 None:intergenic 35.0%
CCCATTGTTACAAAATCCAA+AGG - chr4.2:54161757-54161776 MS.gene027457:CDS 35.0%
GATCCTATCAAAGTACAAGA+AGG + chr4.2:54162012-54162031 None:intergenic 35.0%
GCAGCAACTAAAATTGACAA+TGG - chr4.2:54161295-54161314 MS.gene027457:CDS 35.0%
GGAAAACAAGAAGTCAATAC+TGG + chr4.2:54161991-54162010 None:intergenic 35.0%
GGAAAACATGTTCTGATATG+AGG + chr4.2:54161193-54161212 None:intergenic 35.0%
GTCAAAATTAGGAATCAAGC+TGG - chr4.2:54161133-54161152 MS.gene027457:CDS 35.0%
TGGAAAAGAGCAAGTCAATA+TGG + chr4.2:54160870-54160889 None:intergenic 35.0%
TTGACTACATGGCTAGATTT+GGG + chr4.2:54161532-54161551 None:intergenic 35.0%
TTTAATGGCTTCCTTAAGTG+CGG + chr4.2:54160647-54160666 None:intergenic 35.0%
TTTCTTGTCTCCATCGAAAA+GGG + chr4.2:54161498-54161517 None:intergenic 35.0%
! ATTCGAGCATACGAATCTTT+TGG - chr4.2:54160315-54160334 MS.gene027457:CDS 35.0%
! ATTTTCCTTGGACAAAAGCT+TGG + chr4.2:54160594-54160613 None:intergenic 35.0%
! CAAAATATCTCCCTTTTCGA+TGG - chr4.2:54161485-54161504 MS.gene027457:CDS 35.0%
! CAAAGCATGCATTTTGCATA+TGG - chr4.2:54160683-54160702 MS.gene027457:intron 35.0%
! CACGGAAATTCGATTTTCTT+TGG - chr4.2:54160417-54160436 MS.gene027457:intron 35.0%
! CAGAACATGTTTTCCTATTG+AGG - chr4.2:54161198-54161217 MS.gene027457:intron 35.0%
! GGTGTATCATTTAGAATGTG+CGG - chr4.2:54160786-54160805 MS.gene027457:CDS 35.0%
! TTGGGCATTAGCTTATTTGA+AGG - chr4.2:54160900-54160919 MS.gene027457:CDS 35.0%
!! CAATTGGCTTTGAGGAAAAA+TGG - chr4.2:54159914-54159933 MS.gene027457:CDS 35.0%
!! CGAAAAGGGAGATATTTTGA+AGG + chr4.2:54161484-54161503 None:intergenic 35.0%
!! TGAAGGACTTTTTCTTTGCT+GGG - chr4.2:54160917-54160936 MS.gene027457:CDS 35.0%
!! TGCACAATTTGATTTGTGCA+AGG - chr4.2:54160840-54160859 MS.gene027457:CDS 35.0%
!! TTGAAGGACTTTTTCTTTGC+TGG - chr4.2:54160916-54160935 MS.gene027457:CDS 35.0%
AACCAGCTTTCGACAACAAT+AGG + chr4.2:54160047-54160066 None:intergenic 40.0%
AATCGTTATGATACGCCACT+TGG - chr4.2:54160372-54160391 MS.gene027457:CDS 40.0%
ACAACGCAACATTAACGAAG+TGG - chr4.2:54159825-54159844 MS.gene027457:intron 40.0%
ACATGCTATTCGTGTGCTAA+TGG - chr4.2:54161438-54161457 MS.gene027457:CDS 40.0%
ACCCATCCGGTCTTTATAAA+AGG - chr4.2:54159770-54159789 MS.gene027457:intron 40.0%
AGAAGATTTCTTCCTTTGCG+TGG - chr4.2:54160460-54160479 MS.gene027457:CDS 40.0%
ATACGACTTTGATCAGCAGT+TGG + chr4.2:54160024-54160043 None:intergenic 40.0%
ATCCTATTGTTGTCGAAAGC+TGG - chr4.2:54160042-54160061 MS.gene027457:CDS 40.0%
ATGATACGCCACTTGGAATA+TGG - chr4.2:54160379-54160398 MS.gene027457:CDS 40.0%
CAACATTGCAGTTGACTACA+TGG + chr4.2:54161543-54161562 None:intergenic 40.0%
CATCATACAAAAGTTGACGG+TGG - chr4.2:54161334-54161353 MS.gene027457:CDS 40.0%
CCCATCCGGTCTTTATAAAA+GGG - chr4.2:54159771-54159790 MS.gene027457:intron 40.0%
CGAAAGAGAGTGGAAATCAA+TGG - chr4.2:54160816-54160835 MS.gene027457:CDS 40.0%
CTCATGTAGGAGCAACTTAA+AGG + chr4.2:54161254-54161273 None:intergenic 40.0%
CTTTCTTGTCTCCATCGAAA+AGG + chr4.2:54161499-54161518 None:intergenic 40.0%
GAAGATTTCTTCCTTTGCGT+GGG - chr4.2:54160461-54160480 MS.gene027457:CDS 40.0%
GAGCATATTATGTGGGCATT+TGG - chr4.2:54160285-54160304 MS.gene027457:CDS 40.0%
GAGTCTAAAAAGAACCCATC+CGG - chr4.2:54159757-54159776 MS.gene027457:intron 40.0%
GTTGACTACATGGCTAGATT+TGG + chr4.2:54161533-54161552 None:intergenic 40.0%
TGACTACATGGCTAGATTTG+GGG + chr4.2:54161531-54161550 None:intergenic 40.0%
TGATACGCCACTTGGAATAT+GGG - chr4.2:54160380-54160399 MS.gene027457:CDS 40.0%
TTAATGGCTTCCTTAAGTGC+GGG + chr4.2:54160646-54160665 None:intergenic 40.0%
TTGTGGCAAAGTTGTCAAGT+TGG - chr4.2:54160710-54160729 MS.gene027457:intron 40.0%
! AACTTCCAAGCTTTTGTCCA+AGG - chr4.2:54160586-54160605 MS.gene027457:CDS 40.0%
! AGGAGCAACTTAAAGGCATA+TGG + chr4.2:54161247-54161266 None:intergenic 40.0%
! AGGGAATTTGTTTGTCATCG+TGG - chr4.2:54159790-54159809 MS.gene027457:intron 40.0%
! CCCTTTTATAAAGACCGGAT+GGG + chr4.2:54159774-54159793 None:intergenic 40.0%
! GAATTTGTTTGTCATCGTGG+TGG - chr4.2:54159793-54159812 MS.gene027457:intron 40.0%
! TCCCTTTTATAAAGACCGGA+TGG + chr4.2:54159775-54159794 None:intergenic 40.0%
! TGATTAGTTCCACCACAACT+TGG + chr4.2:54161850-54161869 None:intergenic 40.0%
!! AAGGGAGATATTTTGAAGGG+AGG + chr4.2:54161480-54161499 None:intergenic 40.0%
AGAGAACGAGTTGCTACTAG+AGG - chr4.2:54161628-54161647 MS.gene027457:CDS 45.0%
AGAGGATCAAGACCAAAAGG+AGG - chr4.2:54161787-54161806 MS.gene027457:CDS 45.0%
AGATCAAGATCCCGCACTTA+AGG - chr4.2:54160633-54160652 MS.gene027457:intron 45.0%
CCAAGAGGATCAAGACCAAA+AGG - chr4.2:54161784-54161803 MS.gene027457:CDS 45.0%
CGGATGATTTCGAAAGAGAG+TGG - chr4.2:54160806-54160825 MS.gene027457:CDS 45.0%
GAGGAGTTGAAGCTGCAAAT+AGG - chr4.2:54161806-54161825 MS.gene027457:CDS 45.0%
GATGCAGCATGTTCCTCAAT+AGG + chr4.2:54161214-54161233 None:intergenic 45.0%
GTTGGAATGTTGCGATAAGC+AGG + chr4.2:54160006-54160025 None:intergenic 45.0%
TAGGAATCAAGCTGGAGAAG+AGG - chr4.2:54161141-54161160 MS.gene027457:CDS 45.0%
TGTCATGTTCCAAGTTGTGG+TGG - chr4.2:54161838-54161857 MS.gene027457:CDS 45.0%
TTGCTGGGATGACAACAACA+GGG - chr4.2:54160932-54160951 MS.gene027457:CDS 45.0%
TTTGCTGGGATGACAACAAC+AGG - chr4.2:54160931-54160950 MS.gene027457:CDS 45.0%
! TTCCTTTGCGTGGGCTTTAA+AGG - chr4.2:54160470-54160489 MS.gene027457:CDS 45.0%
!! TACCTTTAAAGCCCACGCAA+AGG + chr4.2:54160475-54160494 None:intergenic 45.0%
!! TGGCTTTGAGGAAAAATGGG+TGG - chr4.2:54159918-54159937 MS.gene027457:CDS 45.0%
!! TGGGCTGGTGTTGATAATCA+CGG - chr4.2:54160399-54160418 MS.gene027457:intron 45.0%
!!! CCTTTTGGTCTTGATCCTCT+TGG + chr4.2:54161787-54161806 None:intergenic 45.0%
AAATCCAAAGGTCGGCCAAG+AGG - chr4.2:54161769-54161788 MS.gene027457:CDS 50.0%
ACACCAGCCCATATTCCAAG+TGG + chr4.2:54160390-54160409 None:intergenic 50.0%
CGCTGTCATGTTCCAAGTTG+TGG - chr4.2:54161835-54161854 MS.gene027457:CDS 50.0%
! GCAGCTTCAACTCCTCCTTT+TGG + chr4.2:54161802-54161821 None:intergenic 50.0%
ACGCCACTTGGAATATGGGC+TGG - chr4.2:54160384-54160403 MS.gene027457:CDS 55.0%
ACGGTGGGCGTCAAGTAAGT+TGG - chr4.2:54161350-54161369 MS.gene027457:CDS 55.0%
CTTGGAACATGACAGCGACG+AGG + chr4.2:54161832-54161851 None:intergenic 55.0%
TGATCCTCTTGGCCGACCTT+TGG + chr4.2:54161776-54161795 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 54159712 54162049 54159712 ID=MS.gene027457
chr4.2 mRNA 54159712 54162049 54159712 ID=MS.gene027457.t1;Parent=MS.gene027457
chr4.2 exon 54161270 54162049 54161270 ID=MS.gene027457.t1.exon1;Parent=MS.gene027457.t1
chr4.2 CDS 54161270 54162049 54161270 ID=cds.MS.gene027457.t1;Parent=MS.gene027457.t1
chr4.2 exon 54160732 54161175 54160732 ID=MS.gene027457.t1.exon2;Parent=MS.gene027457.t1
chr4.2 CDS 54160732 54161175 54160732 ID=cds.MS.gene027457.t1;Parent=MS.gene027457.t1
chr4.2 exon 54160445 54160640 54160445 ID=MS.gene027457.t1.exon3;Parent=MS.gene027457.t1
chr4.2 CDS 54160445 54160640 54160445 ID=cds.MS.gene027457.t1;Parent=MS.gene027457.t1
chr4.2 exon 54159826 54160414 54159826 ID=MS.gene027457.t1.exon4;Parent=MS.gene027457.t1
chr4.2 CDS 54159826 54160414 54159826 ID=cds.MS.gene027457.t1;Parent=MS.gene027457.t1
chr4.2 exon 54159712 54159733 54159712 ID=MS.gene027457.t1.exon5;Parent=MS.gene027457.t1
chr4.2 CDS 54159712 54159733 54159712 ID=cds.MS.gene027457.t1;Parent=MS.gene027457.t1
Gene Sequence

>MS.gene027457

ATGGATTTTCGATCAGGCGCGAACACATTCAAATCTTGTACAAAAGAGTCTAAAAAGAACCCATCCGGTCTTTATAAAAGGGAATTTGTTTGTCATCGTGGTGGTATTGTTAAACAACGCAACATTAACGAAGTGGAATGTCAAAGAAAACGAAAATCTTCAAAATGTAATTGTGGTGTAAAGATGTTGGTTGCTATAAAAACAATTGGCTTTGAGGAAAAATGGGTGGTTACATATTTAAACAATCATCACAATCATGAGTTATTGGATGAAAAAGAGGTTCAATTTCTTCCTGCTTATCGCAACATTCCAACTGCTGATCAAAGTCGTATCCTATTGTTGTCGAAAGCTGGTTGCTCTATAAGTATTATAATGAGAGTGCTTGAGTTAGAGAAAAAAATAGTTACAGGCAATCTACCTTTTCTAGATAAAGATATTCAAAATTTTATTCAATCCGAGAGTTGTATTGGTAAAGAAAATGATGCTTCTGATGGTCTTAAATTATGCAAAAATTTGAAAGATATTGATGATGCCTTCAAATATGAATTCACTATTGATGAAAGTAACAAATTGGAGCATATTATGTGGGCATTTGGTGATTCAATTCGAGCATACGAATCTTTTGGAGATGTTGTAGTTTTTGATACCACGTATAGAATAAATCGTTATGATACGCCACTTGGAATATGGGCTGGTGTTGATAATCACGGAAATTCGATTTTCTTTGGTTGTGTTCTATTGAAGAATGAGAAGATTTCTTCCTTTGCGTGGGCTTTAAAGGTAAATTTTTGTTTACTTAAAGTTGTTATTGTTGTTGAATTTTTAACTTTGAAGCTTTGTTGAAGTCTTTTATATTTATGTTATTATTTTTCAGAACTTCCAAGCTTTTGTCCAAGGAAAATATCCAAAAACTATTCTTACAGATCAAGATCCCGCACTTAAGGAAGCCATTAAAATGGAATTGCCAAACACAAAGCATGCATTTTGCATATGGCATATTGTGGCAAAGTTGTCAAGTTGGTTTTCTTTTGCATTGGGTTCAAGATATAATGAGTTTAAACAAGAGTTTCACAAGGTGTATCATTTAGAATGTGCGGATGATTTCGAAAGAGAGTGGAAATCAATGGTTGCACAATTTGATTTGTGCAAGGATAACCATATTGACTTGCTCTTTTCCAATCTTGAATTTTGGGCATTAGCTTATTTGAAGGACTTTTTCTTTGCTGGGATGACAACAACAGGGCGCTCAGAATCGATAAATGCATATATAAAAAGATTCTTAGGCGCCAATACAAGTTTGACAGATTTTGTAAATCAAGTATGAACTTACTTGAGCTACTTGTTAAAAGTACATTAGTTTTTGAATCATTATCTTATAATTGAGGGATAATTATTATATCTTTGTGTAGGTTGGTGTTGTTGTCAAAATTAGGAATCAAGCTGGAGAAGAGGCAAGAATGCGTCAAAAGTATCACAATCCTCATATCAGAACATGTTTTCCTATTGAGGAACATGCTGCATCTGTACTCACGCCATATGCCTTTAAGTTGCTCCTACATGAGATTGAGTTATCTACAAAATATGCAGCAACTAAAATTGACAATGGTTCTTATATTGTGTAACATCATACAAAAGTTGACGGTGGGCGTCAAGTAAGTTGGATAGTAGAAAAAGAGTCAATTCATTGCTCTTGTAAAGAATTTGAGTTTTCCGGAATATTATGCAGACATGCTATTCGTGTGCTAATGGTGAAAAATTATTTTAGCCTCCCTTCAAAATATCTCCCTTTTCGATGGAGACAAGAAAGTTCATTGATCCCCAAATCTAGCCATGTAGTCAACTGCAATGTTGCTTCCTCTACTAAGTTTCAATCTCTAATTCGATGTTTGGAAGTTGAATCATTAAAGACAAGCGAAAGAGAACGAGTTGCTACTAGAGGATTAGAAAAAGTCATTCAAGATATAAAAGGCATGCCAGAAACTCAAGAATATGTGATTGATTTGGAACATGATGCTCCAAATAACGATGTTTGTGATGTTGAAAATCCCATTGTTACAAAATCCAAAGGTCGGCCAAGAGGATCAAGACCAAAAGGAGGAGTTGAAGCTGCAAATAGGCCTCGTCGCTGTCATGTTCCAAGTTGTGGTGGAACTAATCATGACTCACGAAATTGTCCAAACAAAAAGGAAAAAATTGAAGTATTGCCTTCTCAATCTCCTAATAAGTAAGTTTGGATTGCTATGTGTTGTTCTTTTCATGTCTACATTTATTGAATGCCAGTATTGACTTCTTGTTTTCCTTCTTGTACTTTGATAGGATCATAAAAGGTTGGAAGTAG
Protein sequence

>MS.gene027457.t1

MDFRSGANTFKSCTKESKKNPSGLYKREFVCHRGGIVKQRNINEVECQRKRKSSKCNCGVKMLVAIKTIGFEEKWVVTYLNNHHNHELLDEKEVQFLPAYRNIPTADQSRILLLSKAGCSISIIMRVLELEKKIVTGNLPFLDKDIQNFIQSESCIGKENDASDGLKLCKNLKDIDDAFKYEFTIDESNKLEHIMWAFGDSIRAYESFGDVVVFDTTYRINRYDTPLGIWAGVDNHGNSIFFGCVLLKNEKISSFAWALKNFQAFVQGKYPKTILTDQDPALKEAIKMELPNTKHAFCIWHIVAKLSSWFSFALGSRYNEFKQEFHKVYHLECADDFEREWKSMVAQFDLCKDNHIDLLFSNLEFWALAYLKDFFFAGMTTTGRSESINAYIKRFLGANTSLTDFVNQVGVVVKIRNQAGEEARMRQKYHNPHIRTCFPIEEHAASVLTPYAFKLLLHEIELSTKYAATKIDNVDGGRQVSWIVEKESIHCSCKEFEFSGILCRHAIRVLMVKNYFSLPSKYLPFRWRQESSLIPKSSHVVNCNVASSTKFQSLIRCLEVESLKTSERERVATRGLEKVIQDIKGMPETQEYVIDLEHDAPNNDVCDVENPIVTKSKGRPRGSRPKGGVEAANRPRRCHVPSCGGTNHDSRNCPNKKEKIEVLPSQSPNKIIKGWK