AlfalfaGEDB | MS.gene027457

M. sativa cultivar XinJiangDaYe / MS.gene027457

KEGG: -
GO: GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
PFAM: PF03101.18 PF04434.20 PF10551.12

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027457.t1	RHN81411.1	73.9	679	167	1	8	676	78	756	2.80E-300	1040.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027457.t1	Q9SY66	45.3	591	313	2	23	603	89	679	1.4e-151	538.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027457.t1	A0A396JX52	73.9	679	167	1	8	676	78	756	2.0e-300	1040.8

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene027457.t1	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027457.t1	MTR_3g450850	68.269	208	66	0	9	216	79	286	7.92e-97	302
MS.gene027457.t1	MTR_4g071400	80.992	121	23	0	511	631	1	121	2.77e-63	206
MS.gene027457.t1	MTR_3g012160	96.774	93	3	0	125	217	1	93	6.15e-56	185
MS.gene027457.t1	MTR_4g035820	40.670	209	122	1	26	232	116	324	3.94e-43	166
MS.gene027457.t1	MTR_4g035820	36.129	155	83	2	365	503	326	480	8.93e-21	97.4
MS.gene027457.t1	MTR_6g051425	30.085	236	154	5	27	257	62	291	1.14e-23	103
MS.gene027457.t1	MTR_1g070355	29.665	209	144	3	14	222	104	309	2.01e-21	99.0
MS.gene027457.t1	MTR_3g095940	86.364	44	6	0	430	473	90	133	2.33e-18	82.8
MS.gene027457.t1	MTR_5g083630	65.517	58	20	0	126	183	19	76	1.34e-17	79.3
MS.gene027457.t1	MTR_6g014355	40.708	113	65	1	58	168	14	126	6.38e-16	78.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027457.t1	AT1G10240	45.347	591	313	2	23	603	89	679	0.0	553
MS.gene027457.t1	AT1G10240	45.347	591	313	2	23	603	89	679	0.0	553
MS.gene027457.t1	AT5G28530	40.698	602	328	6	4	584	77	670	2.45e-153	459
MS.gene027457.t1	AT5G28530	40.698	602	328	6	4	584	77	670	2.45e-153	459
MS.gene027457.t1	AT5G28530	40.864	602	331	6	4	584	77	674	1.04e-152	458
MS.gene027457.t1	AT5G28530	35.980	592	307	7	4	584	77	607	3.93e-119	369
MS.gene027457.t1	AT5G28530	36.149	592	310	7	4	584	77	611	1.02e-118	368
MS.gene027457.t1	AT4G38180	30.597	536	338	9	23	534	113	638	1.06e-74	256
MS.gene027457.t1	AT4G15090	26.462	650	440	13	10	632	76	714	8.51e-68	238
MS.gene027457.t1	AT4G15090	26.462	650	440	13	10	632	76	714	8.51e-68	238

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Find 94 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCTGTCAAACTTGTAT+TGG	0.376571	4.2:+54160741	None:intergenic
AAAGAAAATGATGCTTCTGA+TGG	0.590975	4.2:-54161556	MS.gene027457:CDS
AAAGTCATTCAAGATATAAA+AGG	0.358979	4.2:-54160081	MS.gene027457:CDS
AAATCCAAAGGTCGGCCAAG+AGG	0.639624	4.2:-54159970	MS.gene027457:CDS
AAATCTTCAAAATGTAATTG+TGG	0.587616	4.2:-54161874	MS.gene027457:CDS
AACCAGCTTTCGACAACAAT+AGG	0.437905	4.2:+54161695	None:intergenic
AATAGTGAATTCATATTTGA+AGG	0.396961	4.2:+54161495	None:intergenic
AATCGTTATGATACGCCACT+TGG	0.553086	4.2:-54161367	MS.gene027457:CDS
ACAAATTGGAGCATATTATG+TGG	0.542960	4.2:-54161462	MS.gene027457:CDS
ACAACGCAACATTAACGAAG+TGG	0.585119	4.2:-54161914	MS.gene027457:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAAAATGATGCTTCTGA+TGG	-	chr4.2:54160183-54160202	MS.gene027457:CDS	30.0%
ACAAATTGGAGCATATTATG+TGG	-	chr4.2:54160277-54160296	MS.gene027457:CDS	30.0%
ACATCACAAACATCGTTATT+TGG	+	chr4.2:54161730-54161749	None:intergenic	30.0%
ACGAAATTGTCCAAACAAAA+AGG	-	chr4.2:54161876-54161895	MS.gene027457:CDS	30.0%
ACTTAAGGAAGCCATTAAAA+TGG	-	chr4.2:54160648-54160667	MS.gene027457:intron	30.0%
ATCACATATTCTTGAGTTTC+TGG	+	chr4.2:54161688-54161707	None:intergenic	30.0%
CAAATTGGAGCATATTATGT+GGG	-	chr4.2:54160278-54160297	MS.gene027457:CDS	30.0%
GCAATCCAAACTTACTTATT+AGG	+	chr4.2:54161931-54161950	None:intergenic	30.0%
GTTTAAACAAGAGTTTCACA+AGG	-	chr4.2:54160765-54160784	MS.gene027457:CDS	30.0%
TAATGCCCAAAATTCAAGAT+TGG	+	chr4.2:54160890-54160909	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	54159712	54162049	54159712	ID=MS.gene027457
chr4.2	mRNA	54159712	54162049	54159712	ID=MS.gene027457.t1;Parent=MS.gene027457
chr4.2	exon	54161270	54162049	54161270	ID=MS.gene027457.t1.exon1;Parent=MS.gene027457.t1
chr4.2	CDS	54161270	54162049	54161270	ID=cds.MS.gene027457.t1;Parent=MS.gene027457.t1
chr4.2	exon	54160732	54161175	54160732	ID=MS.gene027457.t1.exon2;Parent=MS.gene027457.t1
chr4.2	CDS	54160732	54161175	54160732	ID=cds.MS.gene027457.t1;Parent=MS.gene027457.t1
chr4.2	exon	54160445	54160640	54160445	ID=MS.gene027457.t1.exon3;Parent=MS.gene027457.t1
chr4.2	CDS	54160445	54160640	54160445	ID=cds.MS.gene027457.t1;Parent=MS.gene027457.t1
chr4.2	exon	54159826	54160414	54159826	ID=MS.gene027457.t1.exon4;Parent=MS.gene027457.t1
chr4.2	CDS	54159826	54160414	54159826	ID=cds.MS.gene027457.t1;Parent=MS.gene027457.t1
chr4.2	exon	54159712	54159733	54159712	ID=MS.gene027457.t1.exon5;Parent=MS.gene027457.t1
chr4.2	CDS	54159712	54159733	54159712	ID=cds.MS.gene027457.t1;Parent=MS.gene027457.t1

Gene Sequence

>MS.gene027457

ATGGATTTTCGATCAGGCGCGAACACATTCAAATCTTGTACAAAAGAGTCTAAAAAGAACCCATCCGGTCTTTATAAAAGGGAATTTGTTTGTCATCGTGGTGGTATTGTTAAACAACGCAACATTAACGAAGTGGAATGTCAAAGAAAACGAAAATCTTCAAAATGTAATTGTGGTGTAAAGATGTTGGTTGCTATAAAAACAATTGGCTTTGAGGAAAAATGGGTGGTTACATATTTAAACAATCATCACAATCATGAGTTATTGGATGAAAAAGAGGTTCAATTTCTTCCTGCTTATCGCAACATTCCAACTGCTGATCAAAGTCGTATCCTATTGTTGTCGAAAGCTGGTTGCTCTATAAGTATTATAATGAGAGTGCTTGAGTTAGAGAAAAAAATAGTTACAGGCAATCTACCTTTTCTAGATAAAGATATTCAAAATTTTATTCAATCCGAGAGTTGTATTGGTAAAGAAAATGATGCTTCTGATGGTCTTAAATTATGCAAAAATTTGAAAGATATTGATGATGCCTTCAAATATGAATTCACTATTGATGAAAGTAACAAATTGGAGCATATTATGTGGGCATTTGGTGATTCAATTCGAGCATACGAATCTTTTGGAGATGTTGTAGTTTTTGATACCACGTATAGAATAAATCGTTATGATACGCCACTTGGAATATGGGCTGGTGTTGATAATCACGGAAATTCGATTTTCTTTGGTTGTGTTCTATTGAAGAATGAGAAGATTTCTTCCTTTGCGTGGGCTTTAAAGGTAAATTTTTGTTTACTTAAAGTTGTTATTGTTGTTGAATTTTTAACTTTGAAGCTTTGTTGAAGTCTTTTATATTTATGTTATTATTTTTCAGAACTTCCAAGCTTTTGTCCAAGGAAAATATCCAAAAACTATTCTTACAGATCAAGATCCCGCACTTAAGGAAGCCATTAAAATGGAATTGCCAAACACAAAGCATGCATTTTGCATATGGCATATTGTGGCAAAGTTGTCAAGTTGGTTTTCTTTTGCATTGGGTTCAAGATATAATGAGTTTAAACAAGAGTTTCACAAGGTGTATCATTTAGAATGTGCGGATGATTTCGAAAGAGAGTGGAAATCAATGGTTGCACAATTTGATTTGTGCAAGGATAACCATATTGACTTGCTCTTTTCCAATCTTGAATTTTGGGCATTAGCTTATTTGAAGGACTTTTTCTTTGCTGGGATGACAACAACAGGGCGCTCAGAATCGATAAATGCATATATAAAAAGATTCTTAGGCGCCAATACAAGTTTGACAGATTTTGTAAATCAAGTATGAACTTACTTGAGCTACTTGTTAAAAGTACATTAGTTTTTGAATCATTATCTTATAATTGAGGGATAATTATTATATCTTTGTGTAGGTTGGTGTTGTTGTCAAAATTAGGAATCAAGCTGGAGAAGAGGCAAGAATGCGTCAAAAGTATCACAATCCTCATATCAGAACATGTTTTCCTATTGAGGAACATGCTGCATCTGTACTCACGCCATATGCCTTTAAGTTGCTCCTACATGAGATTGAGTTATCTACAAAATATGCAGCAACTAAAATTGACAATGGTTCTTATATTGTGTAACATCATACAAAAGTTGACGGTGGGCGTCAAGTAAGTTGGATAGTAGAAAAAGAGTCAATTCATTGCTCTTGTAAAGAATTTGAGTTTTCCGGAATATTATGCAGACATGCTATTCGTGTGCTAATGGTGAAAAATTATTTTAGCCTCCCTTCAAAATATCTCCCTTTTCGATGGAGACAAGAAAGTTCATTGATCCCCAAATCTAGCCATGTAGTCAACTGCAATGTTGCTTCCTCTACTAAGTTTCAATCTCTAATTCGATGTTTGGAAGTTGAATCATTAAAGACAAGCGAAAGAGAACGAGTTGCTACTAGAGGATTAGAAAAAGTCATTCAAGATATAAAAGGCATGCCAGAAACTCAAGAATATGTGATTGATTTGGAACATGATGCTCCAAATAACGATGTTTGTGATGTTGAAAATCCCATTGTTACAAAATCCAAAGGTCGGCCAAGAGGATCAAGACCAAAAGGAGGAGTTGAAGCTGCAAATAGGCCTCGTCGCTGTCATGTTCCAAGTTGTGGTGGAACTAATCATGACTCACGAAATTGTCCAAACAAAAAGGAAAAAATTGAAGTATTGCCTTCTCAATCTCCTAATAAGTAAGTTTGGATTGCTATGTGTTGTTCTTTTCATGTCTACATTTATTGAATGCCAGTATTGACTTCTTGTTTTCCTTCTTGTACTTTGATAGGATCATAAAAGGTTGGAAGTAG

Protein sequence

>MS.gene027457.t1

MDFRSGANTFKSCTKESKKNPSGLYKREFVCHRGGIVKQRNINEVECQRKRKSSKCNCGVKMLVAIKTIGFEEKWVVTYLNNHHNHELLDEKEVQFLPAYRNIPTADQSRILLLSKAGCSISIIMRVLELEKKIVTGNLPFLDKDIQNFIQSESCIGKENDASDGLKLCKNLKDIDDAFKYEFTIDESNKLEHIMWAFGDSIRAYESFGDVVVFDTTYRINRYDTPLGIWAGVDNHGNSIFFGCVLLKNEKISSFAWALKNFQAFVQGKYPKTILTDQDPALKEAIKMELPNTKHAFCIWHIVAKLSSWFSFALGSRYNEFKQEFHKVYHLECADDFEREWKSMVAQFDLCKDNHIDLLFSNLEFWALAYLKDFFFAGMTTTGRSESINAYIKRFLGANTSLTDFVNQVGVVVKIRNQAGEEARMRQKYHNPHIRTCFPIEEHAASVLTPYAFKLLLHEIELSTKYAATKIDNVDGGRQVSWIVEKESIHCSCKEFEFSGILCRHAIRVLMVKNYFSLPSKYLPFRWRQESSLIPKSSHVVNCNVASSTKFQSLIRCLEVESLKTSERERVATRGLEKVIQDIKGMPETQEYVIDLEHDAPNNDVCDVENPIVTKSKGRPRGSRPKGGVEAANRPRRCHVPSCGGTNHDSRNCPNKKEKIEVLPSQSPNKIIKGWK