AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027478

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027478.t1 MTR_6g051425 31.273 275 172 5 50 311 19 289 5.11e-37 141
MS.gene027478.t1 MTR_0856s0010 42.767 159 88 2 16 173 4 160 4.31e-33 124
MS.gene027478.t1 MTR_4g035820 28.571 294 188 10 9 288 39 324 1.24e-23 105
MS.gene027478.t1 MTR_0558s0020 26.070 257 166 5 52 284 40 296 2.56e-22 98.2
MS.gene027478.t1 MTR_1g070355 29.787 235 150 7 54 278 80 309 3.84e-21 97.8
MS.gene027478.t1 MTR_8g446020 25.424 236 155 4 52 284 39 256 2.80e-18 85.5
MS.gene027478.t1 MTR_7g446060 46.341 82 44 0 130 211 7 88 5.26e-18 81.3
MS.gene027478.t1 MTR_7g446480 31.977 172 116 1 122 292 143 314 1.05e-17 84.7
MS.gene027478.t1 MTR_7g446510 31.395 172 117 1 122 292 158 329 3.01e-17 83.6
MS.gene027478.t1 MTR_3g450850 25.444 338 225 11 2 320 3 332 6.67e-17 82.8
MS.gene027478.t1 MTR_7g446520 25.424 295 183 7 15 286 35 315 9.90e-17 82.0
MS.gene027478.t1 MTR_1g008290 32.331 133 88 1 108 240 14 144 1.13e-16 77.8
MS.gene027478.t1 MTR_6g022070 28.966 145 99 2 338 482 1 141 1.25e-14 75.9
MS.gene027478.t1 MTR_1g083640 24.561 228 148 5 52 255 55 282 2.24e-14 74.3
MS.gene027478.t1 MTR_5g076070 23.166 259 186 7 23 272 28 282 4.36e-14 73.6
MS.gene027478.t1 MTR_1g013060 23.697 211 150 4 44 244 20 229 6.25e-14 72.0
MS.gene027478.t1 MTR_3g012160 41.975 81 46 1 193 273 14 93 7.29e-14 67.8
MS.gene027478.t1 MTR_6g090505 26.966 178 127 2 109 283 53 230 1.91e-13 72.8
MS.gene027478.t1 MTR_7g115320 55.556 54 21 1 137 190 9 59 2.20e-13 66.2
MS.gene027478.t1 MTR_0402s0040 25.000 200 141 6 28 221 8 204 1.74e-11 67.4
MS.gene027478.t1 MTR_2g105460 23.045 243 160 5 212 451 137 355 3.66e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027478.t1 AT4G38180 34.876 605 359 12 2 584 21 612 2.10e-110 349
MS.gene027478.t1 AT4G15090 33.333 579 367 8 28 595 28 598 7.29e-105 335
MS.gene027478.t1 AT4G15090 33.333 579 367 8 28 595 28 598 7.29e-105 335
MS.gene027478.t1 AT4G15090 33.333 579 367 8 28 595 28 598 7.29e-105 335
MS.gene027478.t1 AT4G15090 33.333 579 367 8 28 595 28 598 7.29e-105 335
MS.gene027478.t1 AT4G15090 33.333 579 367 8 28 595 28 598 7.29e-105 335
MS.gene027478.t1 AT1G52520 32.432 555 354 10 47 593 84 625 5.09e-98 314
MS.gene027478.t1 AT3G22170 31.509 603 358 10 18 595 35 607 1.75e-97 316
MS.gene027478.t1 AT3G22170 31.509 603 358 10 18 595 35 607 1.75e-97 316
MS.gene027478.t1 AT1G76320 31.579 551 348 11 52 593 1 531 4.11e-92 298
MS.gene027478.t1 AT1G76320 31.579 551 348 11 52 593 1 531 2.18e-91 297
MS.gene027478.t1 AT1G76320 31.579 551 348 11 52 593 1 531 2.37e-91 297
MS.gene027478.t1 AT1G76320 31.579 551 348 11 52 593 1 531 2.37e-91 297
MS.gene027478.t1 AT3G06250 33.152 552 317 14 48 595 190 693 3.90e-86 284
MS.gene027478.t1 AT3G06250 33.152 552 317 14 48 595 190 693 3.90e-86 284
MS.gene027478.t1 AT3G06250 33.152 552 317 14 48 595 190 693 3.90e-86 284
MS.gene027478.t1 AT2G27110 31.597 576 356 12 33 604 36 577 3.77e-85 283
MS.gene027478.t1 AT2G27110 31.597 576 356 12 33 604 36 577 3.77e-85 283
MS.gene027478.t1 AT1G80010 30.988 597 382 12 14 595 69 650 6.30e-82 272
MS.gene027478.t1 AT2G27110 35.321 436 262 7 172 604 14 432 6.10e-80 266
MS.gene027478.t1 AT5G18960 32.348 575 333 15 28 595 192 717 6.03e-79 266
MS.gene027478.t1 AT5G18960 32.348 575 333 15 28 595 192 717 6.03e-79 266
MS.gene027478.t1 AT2G32250 29.818 550 333 8 51 595 41 542 8.36e-75 254
MS.gene027478.t1 AT2G32250 29.818 550 333 8 51 595 41 542 1.81e-74 254
MS.gene027478.t1 AT2G32250 29.818 550 333 8 51 595 41 542 1.81e-74 254
MS.gene027478.t1 AT2G32250 29.818 550 333 8 51 595 41 542 1.89e-74 254
MS.gene027478.t1 AT2G32250 29.818 550 333 8 51 595 41 542 1.89e-74 254
MS.gene027478.t1 AT2G32250 29.818 550 333 8 51 595 41 542 1.89e-74 254
MS.gene027478.t1 AT2G32250 29.690 549 333 8 52 595 1 501 8.95e-74 251
MS.gene027478.t1 AT2G32250 29.690 549 333 8 52 595 1 501 9.35e-74 251
MS.gene027478.t1 AT4G38170 33.246 382 244 5 221 600 21 393 7.14e-70 236
MS.gene027478.t1 AT4G19990 29.776 581 335 12 39 595 17 548 7.00e-69 238
MS.gene027478.t1 AT4G19990 29.776 581 335 12 39 595 17 548 7.00e-69 238
MS.gene027478.t1 AT4G19990 29.776 581 335 12 39 595 17 548 7.18e-69 238
MS.gene027478.t1 AT4G19990 29.776 581 335 12 39 595 31 562 8.81e-69 238
MS.gene027478.t1 AT4G19990 29.776 581 335 12 39 595 17 548 1.43e-68 237
MS.gene027478.t1 AT4G19990 29.776 581 335 12 39 595 17 548 1.43e-68 237
MS.gene027478.t1 AT4G19990 30.677 502 296 9 104 595 32 491 4.41e-65 226
MS.gene027478.t1 AT1G10240 26.861 618 407 17 1 595 1 596 2.50e-62 218
MS.gene027478.t1 AT1G10240 26.861 618 407 17 1 595 1 596 2.50e-62 218
MS.gene027478.t1 AT5G28530 28.748 567 356 13 46 584 53 599 4.25e-60 213
MS.gene027478.t1 AT5G28530 28.571 567 353 13 46 584 53 595 1.89e-59 211
MS.gene027478.t1 AT5G28530 28.571 567 353 13 46 584 53 595 1.89e-59 211
MS.gene027478.t1 AT5G28530 24.315 584 349 15 20 584 27 536 5.96e-37 146
MS.gene027478.t1 AT5G28530 24.144 584 346 15 20 584 27 532 2.24e-36 144

Find 109 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTATTTAAAACATGATTTC+TGG 0.164544 4.2:-54645618 MS.gene027478:CDS
TCTTTCCCGCTCTTCGAAAA+TGG 0.249066 4.2:+54645651 None:intergenic
ACAACATTCATTGATAAATA+TGG 0.288122 4.2:-54645700 MS.gene027478:CDS
AAGACCACAACCTAGTAAAA+TGG 0.302758 4.2:+54646005 None:intergenic
ATTTGTTGTTTCAAAGGTAT+TGG 0.312450 4.2:+54646794 None:intergenic
TTCATTGATAAATATGGTTT+GGG 0.319898 4.2:-54645694 MS.gene027478:CDS
ATTCATTGATAAATATGGTT+TGG 0.338141 4.2:-54645695 MS.gene027478:CDS
CGTCTGTGTTGTTGTCATTC+CGG 0.357124 4.2:+54645594 None:intergenic
AGAGGTTCCGTCTAAATTAA+AGG 0.357533 4.2:-54645119 MS.gene027478:CDS
AGCAATGCAGAATGCAATTC+AGG 0.358969 4.2:-54645881 MS.gene027478:CDS
TTGCTTTAAAGAAGGGTCTA+TGG 0.361166 4.2:-54646515 MS.gene027478:CDS
GTACACTCATGAAAAGTATA+TGG 0.364693 4.2:-54645383 MS.gene027478:CDS
ATGCCATTTGCCCCGTTTGT+TGG 0.369173 4.2:-54646048 MS.gene027478:CDS
TGCTGCTCGTGTTGATGTTT+CGG 0.374211 4.2:-54646820 MS.gene027478:CDS
GCAAGCAACCGTGATTTACT+TGG 0.378241 4.2:+54646450 None:intergenic
TTAGAGGAGAGTATTGTCTA+TGG 0.392908 4.2:-54645277 MS.gene027478:CDS
CACCACCTATGCCTAGTATT+AGG 0.393202 4.2:+54645850 None:intergenic
AGTATTGTGTTGATTTCAAC+GGG 0.398148 4.2:-54645237 MS.gene027478:CDS
CTAGGCATAGGTGGTGTCTA+TGG 0.405638 4.2:-54645843 MS.gene027478:CDS
GGCTAAGGTTTATGTCTTCA+AGG 0.406555 4.2:-54645936 MS.gene027478:CDS
TCTCCATCAGGGGCTAATAA+AGG 0.410771 4.2:+54646855 None:intergenic
TTATTTAAAACATGATTTCT+GGG 0.414268 4.2:-54645617 MS.gene027478:CDS
AGTAAATCACGGTTGCTTGC+TGG 0.418839 4.2:-54646447 MS.gene027478:CDS
TAAATTACTTTGCTAGAATG+AGG 0.430378 4.2:-54646224 MS.gene027478:CDS
TCAAGCACAATCATTCCATT+CGG 0.432592 4.2:-54645442 MS.gene027478:CDS
GAGCAATCAACTTTCTTCGT+TGG 0.434641 4.2:+54646552 None:intergenic
GTCATATTTGTTTGTCAAGT+AGG 0.436248 4.2:+54646071 None:intergenic
ATGTTACGTGCACTTGTTTG+CGG 0.437578 4.2:-54645204 MS.gene027478:CDS
GATGATTTACACCAACAAAC+GGG 0.438849 4.2:+54646037 None:intergenic
CAAGCACAATCATTCCATTC+GGG 0.443171 4.2:-54645441 MS.gene027478:CDS
ACACCAACAAACGGGGCAAA+TGG 0.443695 4.2:+54646045 None:intergenic
CTTGTTTGATAGCATATTCA+CGG 0.444216 4.2:+54646697 None:intergenic
ATGGTTTGGGTGAAAATGAT+TGG 0.445440 4.2:-54645681 MS.gene027478:CDS
ACACAATACTTACTTTCGTT+CGG 0.447936 4.2:+54645250 None:intergenic
AAAGGATGATCGATCAAGAT+AGG 0.456814 4.2:+54646873 None:intergenic
AAAACTAGAAACTCTAAGAA+AGG 0.460549 4.2:-54646663 MS.gene027478:CDS
ACTCCAAGACAAGGCTGAAA+AGG 0.462412 4.2:-54645488 MS.gene027478:CDS
AGGTTGTGTTTCCTAATACT+AGG 0.465284 4.2:-54645861 MS.gene027478:CDS
TTAAAACATGATTTCTGGGC+CGG 0.468973 4.2:-54645613 MS.gene027478:CDS
GATAACGGTTGCTTTAAAGA+AGG 0.473052 4.2:-54646523 MS.gene027478:CDS
GAGAGATGTCAGAAACTTTG+TGG 0.474597 4.2:-54646295 MS.gene027478:CDS
TTTGTTGGTGTAAATCATCA+TGG 0.480095 4.2:-54646033 MS.gene027478:CDS
CCGTCATCTAGCTCTCCATC+AGG 0.480197 4.2:+54646843 None:intergenic
CACTTATGCTCGATGGCTGA+TGG 0.482201 4.2:-54646900 None:intergenic
TCGGGAAGGAAGGAGATGGC+AGG 0.482875 4.2:-54646254 MS.gene027478:CDS
GACATCATTTGTTGTTTCAA+AGG 0.486604 4.2:+54646788 None:intergenic
TGATGATTTACACCAACAAA+CGG 0.495743 4.2:+54646036 None:intergenic
ACCACCGGCATCACATATCA+TGG 0.496961 4.2:+54646341 None:intergenic
TTGAAATTGTCTCTCGATTG+TGG 0.499701 4.2:+54645411 None:intergenic
TATGTCTTCAAGGCCTCCAA+TGG 0.500495 4.2:-54645926 MS.gene027478:CDS
TTAAAAGACATACCGATAGT+AGG 0.501742 4.2:+54646747 None:intergenic
TTTCGAAGAGCGGGAAAGAT+GGG 0.502201 4.2:-54645647 MS.gene027478:CDS
GTTGGATTCGTCTTCAAAGT+TGG 0.502236 4.2:+54646570 None:intergenic
CATCTCCTTCCTTCCCGAGA+AGG 0.502837 4.2:+54646259 None:intergenic
CGTGAATATGCTATCAAACA+AGG 0.505903 4.2:-54646696 MS.gene027478:CDS
TTACGAAAATCTAACATTTG+TGG 0.512305 4.2:-54646319 MS.gene027478:CDS
AAACGATCAGGCAGGTGTAC+GGG 0.512319 4.2:-54646382 MS.gene027478:CDS
TCAGTTACAATTCCCATTGG+AGG 0.514879 4.2:+54645913 None:intergenic
TCTGGGCTGATGCACGTAGT+AGG 0.516810 4.2:-54646137 MS.gene027478:CDS
ATAACGGTTGCTTTAAAGAA+GGG 0.522275 4.2:-54646522 MS.gene027478:CDS
AAACTAGAAACTCTAAGAAA+GGG 0.523644 4.2:-54646662 MS.gene027478:CDS
CGTCATCTAGCTCTCCATCA+GGG 0.529370 4.2:+54646844 None:intergenic
GGATTCGTCTTCAAAGTTGG+TGG 0.530111 4.2:+54646573 None:intergenic
CTATGAGATTGACATGGACG+AGG 0.532418 4.2:-54646181 MS.gene027478:CDS
TAAACGATCAGGCAGGTGTA+CGG 0.533293 4.2:-54646383 MS.gene027478:CDS
GGTTGTTCAAAACATGGCTA+AGG 0.534319 4.2:-54645951 MS.gene027478:CDS
TTGTGTCTATGATTCTCTGT+TGG 0.536475 4.2:-54645746 MS.gene027478:CDS
CATCCTTTATTAGCCCCTGA+TGG 0.541554 4.2:-54646858 MS.gene027478:CDS
TGTCGCACCTTTAATTTAGA+CGG 0.542474 4.2:+54645112 None:intergenic
AACATCACCAAAGTACTCAT+AGG 0.543531 4.2:+54646110 None:intergenic
TGATCAGTTACAATTCCCAT+TGG 0.544868 4.2:+54645910 None:intergenic
AACTCTAAGAAAGGGTCAGA+TGG 0.545893 4.2:-54646654 MS.gene027478:CDS
TGGTCATCTAAGAGTGAAAG+AGG 0.546898 4.2:-54645137 MS.gene027478:CDS
ATGTCTTCAAGGCCTCCAAT+GGG 0.548123 4.2:-54645925 MS.gene027478:CDS
AAGTATTGTGTTGATTTCAA+CGG 0.550175 4.2:-54645238 MS.gene027478:CDS
TTGGTGGTATGTTTGATTCG+TGG 0.550924 4.2:+54646589 None:intergenic
AGACACCTTCTCGGGAAGGA+AGG 0.553011 4.2:-54646264 MS.gene027478:CDS
TGATTGCTCAGCTAAGATAA+CGG 0.560773 4.2:-54646538 MS.gene027478:CDS
AAATTACTTTGCTAGAATGA+GGG 0.563092 4.2:-54646223 MS.gene027478:CDS
ATTCTAACATGTGCAAGAGA+AGG 0.566543 4.2:-54646615 MS.gene027478:CDS
CGGCATCACATATCATGGAA+TGG 0.566884 4.2:+54646346 None:intergenic
TGTTTCCTAATACTAGGCAT+AGG 0.571109 4.2:-54645855 MS.gene027478:CDS
TTACCTGGTTGTTCAAAACA+TGG 0.574699 4.2:-54645957 MS.gene027478:CDS
GATGAATTCACGCCTACTAT+CGG 0.575926 4.2:-54646759 MS.gene027478:CDS
AGGTCTGCCTATGAGTACTT+TGG 0.575958 4.2:-54646117 MS.gene027478:CDS
TCTCGAAGGTGTGACTACAT+TGG 0.577648 4.2:-54645323 MS.gene027478:CDS
AAGATACTAGGTCCTTTACC+TGG 0.580541 4.2:-54645972 MS.gene027478:CDS
TTGTTGGTGTAAATCATCAT+GGG 0.583689 4.2:-54646032 MS.gene027478:CDS
ATATTTGTTTGTCAAGTAGG+TGG 0.595151 4.2:+54646074 None:intergenic
AAAGGATACGCACAGTACAA+TGG 0.596624 4.2:-54645790 MS.gene027478:CDS
GGTGTCTATGGCATATAATG+AGG 0.597420 4.2:-54645831 MS.gene027478:CDS
CATTCCATGATATGTGATGC+CGG 0.601269 4.2:-54646345 MS.gene027478:CDS
CGACAACGCACTCCAAGACA+AGG 0.606607 4.2:-54645497 MS.gene027478:CDS
AGAAAGACACCTTCTCGGGA+AGG 0.609091 4.2:-54646268 MS.gene027478:CDS
TTCCTAATACTAGGCATAGG+TGG 0.615209 4.2:-54645852 MS.gene027478:CDS
CGGGAAGGAAGGAGATGGCA+GGG 0.622349 4.2:-54646253 MS.gene027478:CDS
GTCATCTAGCTCTCCATCAG+GGG 0.623144 4.2:+54646845 None:intergenic
ATTGTGGAATTTGACCCGAA+TGG 0.624996 4.2:+54645427 None:intergenic
GGATGATCGATCAAGATAGG+AGG 0.629981 4.2:+54646876 None:intergenic
CTTCTCGGGAAGGAAGGAGA+TGG 0.631777 4.2:-54646258 MS.gene027478:CDS
GTGAATATGCTATCAAACAA+GGG 0.632509 4.2:-54646695 MS.gene027478:CDS
AAATACAACTGTTATCTCGA+AGG 0.637450 4.2:-54645337 MS.gene027478:CDS
CAAGGCTGAAAAGGAATACG+AGG 0.638254 4.2:-54645479 MS.gene027478:CDS
AGATAAGTCCAAGTAAATCA+CGG 0.638280 4.2:-54646458 MS.gene027478:CDS
ATGATTTACACCAACAAACG+GGG 0.651499 4.2:+54646038 None:intergenic
CCTGATGGAGAGCTAGATGA+CGG 0.653770 4.2:-54646843 MS.gene027478:CDS
AGATTGACATGCATGCACAG+CGG 0.780563 4.2:-54646416 MS.gene027478:CDS
TCCATGATATGTGATGCCGG+TGG 0.781122 4.2:-54646342 MS.gene027478:CDS
GTATTGTGTTGATTTCAACG+GGG 0.859785 4.2:-54645236 MS.gene027478:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATCATGTTTTAAATAACA+TGG + chr4.2:54646365-54646384 None:intergenic 15.0%
!!! TTATTTAAAACATGATTTCT+GGG - chr4.2:54646367-54646386 MS.gene027478:CDS 15.0%
!! ACAACATTCATTGATAAATA+TGG - chr4.2:54646284-54646303 MS.gene027478:CDS 20.0%
!! ATTCATTGATAAATATGGTT+TGG - chr4.2:54646289-54646308 MS.gene027478:CDS 20.0%
!! TTCATTGATAAATATGGTTT+GGG - chr4.2:54646290-54646309 MS.gene027478:CDS 20.0%
!!! GTTATTTAAAACATGATTTC+TGG - chr4.2:54646366-54646385 MS.gene027478:CDS 20.0%
! AAAATTGCCGAAAAATTGAA+AGG - chr4.2:54646176-54646195 MS.gene027478:CDS 25.0%
! AAATTACTTTGCTAGAATGA+GGG - chr4.2:54645761-54645780 MS.gene027478:CDS 25.0%
! TAAATTACTTTGCTAGAATG+AGG - chr4.2:54645760-54645779 MS.gene027478:CDS 25.0%
! TTACGAAAATCTAACATTTG+TGG - chr4.2:54645665-54645684 MS.gene027478:CDS 25.0%
!! AAAACTAGAAACTCTAAGAA+AGG - chr4.2:54645321-54645340 MS.gene027478:CDS 25.0%
!! AAACTAGAAACTCTAAGAAA+GGG - chr4.2:54645322-54645341 MS.gene027478:CDS 25.0%
!! ATCATAGACACAAATTTTCA+TGG + chr4.2:54646231-54646250 None:intergenic 25.0%
!! TATCGGTATGTCTTTTAAAT+CGG - chr4.2:54645242-54645261 MS.gene027478:CDS 25.0%
!!! AAGTATTGTGTTGATTTCAA+CGG - chr4.2:54646746-54646765 MS.gene027478:CDS 25.0%
!!! ATTTGTTGTTTCAAAGGTAT+TGG + chr4.2:54645193-54645212 None:intergenic 25.0%
!!! TGGTTGATTATTTTGTTGTT+GGG - chr4.2:54646258-54646277 MS.gene027478:CDS 25.0%
!!! TTGGTTGATTATTTTGTTGT+TGG - chr4.2:54646257-54646276 MS.gene027478:CDS 25.0%
AAATACAACTGTTATCTCGA+AGG - chr4.2:54646647-54646666 MS.gene027478:CDS 30.0%
ACACAATACTTACTTTCGTT+CGG + chr4.2:54646737-54646756 None:intergenic 30.0%
AGATAAGTCCAAGTAAATCA+CGG - chr4.2:54645526-54645545 MS.gene027478:CDS 30.0%
ATATTTGTTTGTCAAGTAGG+TGG + chr4.2:54645913-54645932 None:intergenic 30.0%
GTCATATTTGTTTGTCAAGT+AGG + chr4.2:54645916-54645935 None:intergenic 30.0%
GTGAATATGCTATCAAACAA+GGG - chr4.2:54645289-54645308 MS.gene027478:CDS 30.0%
TGATGATTTACACCAACAAA+CGG + chr4.2:54645951-54645970 None:intergenic 30.0%
TTAAAAGACATACCGATAGT+AGG + chr4.2:54645240-54645259 None:intergenic 30.0%
TTGTTGGTGTAAATCATCAT+GGG - chr4.2:54645952-54645971 MS.gene027478:CDS 30.0%
TTTGTTGGTGTAAATCATCA+TGG - chr4.2:54645951-54645970 MS.gene027478:CDS 30.0%
! CTTGTTTGATAGCATATTCA+CGG + chr4.2:54645290-54645309 None:intergenic 30.0%
! CTTTTTCTATGAGATTGACA+TGG - chr4.2:54645797-54645816 MS.gene027478:CDS 30.0%
! GTACACTCATGAAAAGTATA+TGG - chr4.2:54646601-54646620 MS.gene027478:CDS 30.0%
! TCATGTGCAGAATATTTTCT+GGG - chr4.2:54645830-54645849 MS.gene027478:CDS 30.0%
! TGCGTATCCTTTCAATTTTT+CGG + chr4.2:54646186-54646205 None:intergenic 30.0%
! TTCATGTGCAGAATATTTTC+TGG - chr4.2:54645829-54645848 MS.gene027478:CDS 30.0%
! TTTTATCAGCTGAAGATACT+AGG - chr4.2:54646000-54646019 MS.gene027478:CDS 30.0%
!! AGTATTGTGTTGATTTCAAC+GGG - chr4.2:54646747-54646766 MS.gene027478:CDS 30.0%
!! ATAACGGTTGCTTTAAAGAA+GGG - chr4.2:54645462-54645481 MS.gene027478:CDS 30.0%
!! ATCAACATACACTGTTTTAG+AGG - chr4.2:54646691-54646710 MS.gene027478:CDS 30.0%
!! GACAATTTTGATAAACGATC+AGG - chr4.2:54645590-54645609 MS.gene027478:CDS 30.0%
!! GACATCATTTGTTGTTTCAA+AGG + chr4.2:54645199-54645218 None:intergenic 30.0%
!!! TGTATGAATGCTTTTTTCGA+TGG - chr4.2:54646419-54646438 MS.gene027478:CDS 30.0%
!!! TTGATTATTTTGTTGTTGGG+TGG - chr4.2:54646261-54646280 MS.gene027478:CDS 30.0%
AAAAAGAAAGACACCTTCTC+GGG - chr4.2:54645712-54645731 MS.gene027478:CDS 35.0%
AAAGGATGATCGATCAAGAT+AGG + chr4.2:54645114-54645133 None:intergenic 35.0%
AAGACCACAACCTAGTAAAA+TGG + chr4.2:54645982-54646001 None:intergenic 35.0%
AGAGGTTCCGTCTAAATTAA+AGG - chr4.2:54646865-54646884 MS.gene027478:CDS 35.0%
AGGTTGTGTTTCCTAATACT+AGG - chr4.2:54646123-54646142 MS.gene027478:CDS 35.0%
ATGATTTACACCAACAAACG+GGG + chr4.2:54645949-54645968 None:intergenic 35.0%
ATTCTAACATGTGCAAGAGA+AGG - chr4.2:54645369-54645388 MS.gene027478:CDS 35.0%
CGTGAATATGCTATCAAACA+AGG - chr4.2:54645288-54645307 MS.gene027478:CDS 35.0%
GAAAAAGAAAGACACCTTCT+CGG - chr4.2:54645711-54645730 MS.gene027478:CDS 35.0%
GATGATTTACACCAACAAAC+GGG + chr4.2:54645950-54645969 None:intergenic 35.0%
TCAAGCACAATCATTCCATT+CGG - chr4.2:54646542-54646561 MS.gene027478:CDS 35.0%
TGATCAGTTACAATTCCCAT+TGG + chr4.2:54646077-54646096 None:intergenic 35.0%
TGATTGCTCAGCTAAGATAA+CGG - chr4.2:54645446-54645465 MS.gene027478:CDS 35.0%
TGTTTCCTAATACTAGGCAT+AGG - chr4.2:54646129-54646148 MS.gene027478:CDS 35.0%
TTACCTGGTTGTTCAAAACA+TGG - chr4.2:54646027-54646046 MS.gene027478:CDS 35.0%
TTGAAATTGTCTCTCGATTG+TGG + chr4.2:54646576-54646595 None:intergenic 35.0%
TTGTGTCTATGATTCTCTGT+TGG - chr4.2:54646238-54646257 MS.gene027478:CDS 35.0%
! AACATCACCAAAGTACTCAT+AGG + chr4.2:54645877-54645896 None:intergenic 35.0%
! ATGGTTTGGGTGAAAATGAT+TGG - chr4.2:54646303-54646322 MS.gene027478:CDS 35.0%
! CAATCCATTTTACTAGGTTG+TGG - chr4.2:54645975-54645994 MS.gene027478:CDS 35.0%
! GGTATGTCTTTTAAATCGGT+TGG - chr4.2:54645246-54645265 MS.gene027478:CDS 35.0%
! TGAATACCATTTTCGAAGAG+CGG - chr4.2:54646327-54646346 MS.gene027478:CDS 35.0%
! TGTCGCACCTTTAATTTAGA+CGG + chr4.2:54646875-54646894 None:intergenic 35.0%
! TGTTAGATTTTCGTAACCAC+CGG + chr4.2:54645661-54645680 None:intergenic 35.0%
! TTAAAACATGATTTCTGGGC+CGG - chr4.2:54646371-54646390 MS.gene027478:CDS 35.0%
!! AGACATTCACTTTTCGTACT+TGG - chr4.2:54646827-54646846 MS.gene027478:CDS 35.0%
!! ATTTTGATAAACGATCAGGC+AGG - chr4.2:54645594-54645613 MS.gene027478:CDS 35.0%
!! GATAACGGTTGCTTTAAAGA+AGG - chr4.2:54645461-54645480 MS.gene027478:CDS 35.0%
!! GTATTGTGTTGATTTCAACG+GGG - chr4.2:54646748-54646767 MS.gene027478:CDS 35.0%
!! TGTTTTGAACAACCAGGTAA+AGG + chr4.2:54646027-54646046 None:intergenic 35.0%
!! TTAGAGGAGAGTATTGTCTA+TGG - chr4.2:54646707-54646726 MS.gene027478:CDS 35.0%
!! TTGCTTTAAAGAAGGGTCTA+TGG - chr4.2:54645469-54645488 MS.gene027478:CDS 35.0%
AAAGGATACGCACAGTACAA+TGG - chr4.2:54646194-54646213 MS.gene027478:CDS 40.0%
AAGATACTAGGTCCTTTACC+TGG - chr4.2:54646012-54646031 MS.gene027478:CDS 40.0%
AGCAATGCAGAATGCAATTC+AGG - chr4.2:54646103-54646122 MS.gene027478:CDS 40.0%
ATGTTACGTGCACTTGTTTG+CGG - chr4.2:54646780-54646799 MS.gene027478:CDS 40.0%
ATTGTGGAATTTGACCCGAA+TGG + chr4.2:54646560-54646579 None:intergenic 40.0%
CAAGCACAATCATTCCATTC+GGG - chr4.2:54646543-54646562 MS.gene027478:CDS 40.0%
CATTCCATGATATGTGATGC+CGG - chr4.2:54645639-54645658 MS.gene027478:CDS 40.0%
GAGAGATGTCAGAAACTTTG+TGG - chr4.2:54645689-54645708 MS.gene027478:CDS 40.0%
GAGCAATCAACTTTCTTCGT+TGG + chr4.2:54645435-54645454 None:intergenic 40.0%
GATGAATTCACGCCTACTAT+CGG - chr4.2:54645225-54645244 MS.gene027478:CDS 40.0%
GGCTAAGGTTTATGTCTTCA+AGG - chr4.2:54646048-54646067 MS.gene027478:CDS 40.0%
GGTGTCTATGGCATATAATG+AGG - chr4.2:54646153-54646172 MS.gene027478:CDS 40.0%
GGTTGTTCAAAACATGGCTA+AGG - chr4.2:54646033-54646052 MS.gene027478:CDS 40.0%
GTTGGATTCGTCTTCAAAGT+TGG + chr4.2:54645417-54645436 None:intergenic 40.0%
TCAGTTACAATTCCCATTGG+AGG + chr4.2:54646074-54646093 None:intergenic 40.0%
TGGTCATCTAAGAGTGAAAG+AGG - chr4.2:54646847-54646866 MS.gene027478:CDS 40.0%
TTCCTAATACTAGGCATAGG+TGG - chr4.2:54646132-54646151 MS.gene027478:CDS 40.0%
! AACTCTAAGAAAGGGTCAGA+TGG - chr4.2:54645330-54645349 MS.gene027478:CDS 40.0%
! AGGAATACGAGGCAGATTTT+CGG - chr4.2:54646516-54646535 MS.gene027478:CDS 40.0%
! CATGGGCAATCCATTTTACT+AGG - chr4.2:54645969-54645988 MS.gene027478:CDS 40.0%
! GAATACCATTTTCGAAGAGC+GGG - chr4.2:54646328-54646347 MS.gene027478:CDS 40.0%
! TTGGTGGTATGTTTGATTCG+TGG + chr4.2:54645398-54645417 None:intergenic 40.0%
!! ATTCCTTTTCAGCCTTGTCT+TGG + chr4.2:54646502-54646521 None:intergenic 40.0%
!! TAGCCATGTTTTGAACAACC+AGG + chr4.2:54646033-54646052 None:intergenic 40.0%
ACTCCAAGACAAGGCTGAAA+AGG - chr4.2:54646496-54646515 MS.gene027478:CDS 45.0%
AGATTGACATGCATGCACAG+CGG - chr4.2:54645568-54645587 MS.gene027478:CDS 45.0%
AGGTCTGCCTATGAGTACTT+TGG - chr4.2:54645867-54645886 MS.gene027478:CDS 45.0%
AGTAAATCACGGTTGCTTGC+TGG - chr4.2:54645537-54645556 MS.gene027478:CDS 45.0%
ATGTCTTCAAGGCCTCCAAT+GGG - chr4.2:54646059-54646078 MS.gene027478:CDS 45.0%
CAAGGCTGAAAAGGAATACG+AGG - chr4.2:54646505-54646524 MS.gene027478:CDS 45.0%
CACCACCTATGCCTAGTATT+AGG + chr4.2:54646137-54646156 None:intergenic 45.0%
CATCCTTTATTAGCCCCTGA+TGG - chr4.2:54645126-54645145 MS.gene027478:CDS 45.0%
CGGCATCACATATCATGGAA+TGG + chr4.2:54645641-54645660 None:intergenic 45.0%
CTATGAGATTGACATGGACG+AGG - chr4.2:54645803-54645822 MS.gene027478:CDS 45.0%
GCAAGCAACCGTGATTTACT+TGG + chr4.2:54645537-54645556 None:intergenic 45.0%
GGATGATCGATCAAGATAGG+AGG + chr4.2:54645111-54645130 None:intergenic 45.0%
GGATTCGTCTTCAAAGTTGG+TGG + chr4.2:54645414-54645433 None:intergenic 45.0%
TAAACGATCAGGCAGGTGTA+CGG - chr4.2:54645601-54645620 MS.gene027478:CDS 45.0%
TATGTCTTCAAGGCCTCCAA+TGG - chr4.2:54646058-54646077 MS.gene027478:CDS 45.0%
TCTCCATCAGGGGCTAATAA+AGG + chr4.2:54645132-54645151 None:intergenic 45.0%
TCTTTCCCGCTCTTCGAAAA+TGG + chr4.2:54646336-54646355 None:intergenic 45.0%
TTTCGAAGAGCGGGAAAGAT+GGG - chr4.2:54646337-54646356 MS.gene027478:CDS 45.0%
! CGTCTGTGTTGTTGTCATTC+CGG + chr4.2:54646393-54646412 None:intergenic 45.0%
! TCTCGAAGGTGTGACTACAT+TGG - chr4.2:54646661-54646680 MS.gene027478:CDS 45.0%
! TGCTGCTCGTGTTGATGTTT+CGG - chr4.2:54645164-54645183 MS.gene027478:CDS 45.0%
! TTTTCGAAGAGCGGGAAAGA+TGG - chr4.2:54646336-54646355 MS.gene027478:CDS 45.0%
AAACGATCAGGCAGGTGTAC+GGG - chr4.2:54645602-54645621 MS.gene027478:CDS 50.0%
ACACCAACAAACGGGGCAAA+TGG + chr4.2:54645942-54645961 None:intergenic 50.0%
ACCACCGGCATCACATATCA+TGG + chr4.2:54645646-54645665 None:intergenic 50.0%
AGAAAGACACCTTCTCGGGA+AGG - chr4.2:54645716-54645735 MS.gene027478:CDS 50.0%
CCTGATGGAGAGCTAGATGA+CGG - chr4.2:54645141-54645160 MS.gene027478:CDS 50.0%
CTAGGCATAGGTGGTGTCTA+TGG - chr4.2:54646141-54646160 MS.gene027478:CDS 50.0%
TCCATGATATGTGATGCCGG+TGG - chr4.2:54645642-54645661 MS.gene027478:CDS 50.0%
! ATGCCATTTGCCCCGTTTGT+TGG - chr4.2:54645936-54645955 MS.gene027478:CDS 50.0%
!! CGTCATCTAGCTCTCCATCA+GGG + chr4.2:54645143-54645162 None:intergenic 50.0%
!! GTCATCTAGCTCTCCATCAG+GGG + chr4.2:54645142-54645161 None:intergenic 50.0%
AGACACCTTCTCGGGAAGGA+AGG - chr4.2:54645720-54645739 MS.gene027478:CDS 55.0%
CATCTCCTTCCTTCCCGAGA+AGG + chr4.2:54645728-54645747 None:intergenic 55.0%
CGACAACGCACTCCAAGACA+AGG - chr4.2:54646487-54646506 MS.gene027478:CDS 55.0%
CTTCTCGGGAAGGAAGGAGA+TGG - chr4.2:54645726-54645745 MS.gene027478:CDS 55.0%
TCTGGGCTGATGCACGTAGT+AGG - chr4.2:54645847-54645866 MS.gene027478:CDS 55.0%
!! CCGTCATCTAGCTCTCCATC+AGG + chr4.2:54645144-54645163 None:intergenic 55.0%
CGGGAAGGAAGGAGATGGCA+GGG - chr4.2:54645731-54645750 MS.gene027478:CDS 60.0%
TCGGGAAGGAAGGAGATGGC+AGG - chr4.2:54645730-54645749 MS.gene027478:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 54645096 54646910 54645096 ID=MS.gene027478
chr4.2 mRNA 54645096 54646910 54645096 ID=MS.gene027478.t1;Parent=MS.gene027478
chr4.2 exon 54645096 54646910 54645096 ID=MS.gene027478.t1.exon1;Parent=MS.gene027478.t1
chr4.2 CDS 54645096 54646910 54645096 ID=cds.MS.gene027478.t1;Parent=MS.gene027478.t1
Gene Sequence

>MS.gene027478

ATGGCTGATGGTCCTCCTATCTTGATCGATCATCCTTTATTAGCCCCTGATGGAGAGCTAGATGACGGTGCTGCTCGTGTTGATGTTTCGGATTCCAATACCTTTGAAACAACAAATGATGTCGCAACAGATGAATTCACGCCTACTATCGGTATGTCTTTTAAATCGGTTGGAGATTGTAAGTCTTTTTACCGTGAATATGCTATCAAACAAGGGTTTGCGATTAAAACTAGAAACTCTAAGAAAGGGTCAGATGGCATGTTAAGATATTTCATTCTAACATGTGCAAGAGAAGGTCACCACGAATCAAACATACCACCAACTTTGAAGACGAATCCAACGAAGAAAGTTGATTGCTCAGCTAAGATAACGGTTGCTTTAAAGAAGGGTCTATGGACTATTTTGTCTTTTAATTCATGTCATAATCATGAGATAAGTCCAAGTAAATCACGGTTGCTTGCTGGCAATAAGAAGATTGACATGCATGCACAGCGGACAATTTTGATAAACGATCAGGCAGGTGTACGGGTGAACAAAAGTTTCCATTCCATGATATGTGATGCCGGTGGTTACGAAAATCTAACATTTGTGGAGAGAGATGTCAGAAACTTTGTGGAAAAAGAAAGACACCTTCTCGGGAAGGAAGGAGATGGCAGGGCACTTTTAAATTACTTTGCTAGAATGAGGGAATGCAACAAAGACTTTTTCTATGAGATTGACATGGACGAGGAATTTCATGTGCAGAATATTTTCTGGGCTGATGCACGTAGTAGGTCTGCCTATGAGTACTTTGGTGATGTTGTCACGTTTGATACCACCTACTTGACAAACAAATATGACATGCCATTTGCCCCGTTTGTTGGTGTAAATCATCATGGGCAATCCATTTTACTAGGTTGTGGTCTTTTATCAGCTGAAGATACTAGGTCCTTTACCTGGTTGTTCAAAACATGGCTAAGGTTTATGTCTTCAAGGCCTCCAATGGGAATTGTAACTGATCAGTGCAAAGCAATGCAGAATGCAATTCAGGTTGTGTTTCCTAATACTAGGCATAGGTGGTGTCTATGGCATATAATGAGGAAAATTGCCGAAAAATTGAAAGGATACGCACAGTACAATGGAATAAAAAATGCCATGAAAATTTGTGTCTATGATTCTCTGTTGGTTGATTATTTTGTTGTTGGGTGGACAACATTCATTGATAAATATGGTTTGGGTGAAAATGATTGGTTGAATACCATTTTCGAAGAGCGGGAAAGATGGGTGCCATGTTATTTAAAACATGATTTCTGGGCCGGAATGACAACAACACAGACGAGTGAATGTATGAATGCTTTTTTCGATGGTTACATTAATGCAAGAACTTCTCTCAGCGAATTTGTAAAGCAATACGACAACGCACTCCAAGACAAGGCTGAAAAGGAATACGAGGCAGATTTTCGGTCTTCAAGCACAATCATTCCATTCGGGTCAAATTCCACAATCGAGAGACAATTTCAAGCTGAGTACACTCATGAAAAGTATATGGAGATACAAGTAGAATTTCGTGAGAAATACAACTGTTATCTCGAAGGTGTGACTACATTGGAATTTACATCAACATACACTGTTTTAGAGGAGAGTATTGTCTATGGTAAACCGAACGAAAGTAAGTATTGTGTTGATTTCAACGGGGAAGACAATAATGTTACGTGCACTTGTTTGCGGTTTGAGTTCAGATGCGTTTTGTGTAGACATTCACTTTTCGTACTTGGTCATCTAAGAGTGAAAGAGGTTCCGTCTAAATTAAAGGTGCGACATTGTCTTACTAATTAA
Protein sequence

>MS.gene027478.t1

MADGPPILIDHPLLAPDGELDDGAARVDVSDSNTFETTNDVATDEFTPTIGMSFKSVGDCKSFYREYAIKQGFAIKTRNSKKGSDGMLRYFILTCAREGHHESNIPPTLKTNPTKKVDCSAKITVALKKGLWTILSFNSCHNHEISPSKSRLLAGNKKIDMHAQRTILINDQAGVRVNKSFHSMICDAGGYENLTFVERDVRNFVEKERHLLGKEGDGRALLNYFARMRECNKDFFYEIDMDEEFHVQNIFWADARSRSAYEYFGDVVTFDTTYLTNKYDMPFAPFVGVNHHGQSILLGCGLLSAEDTRSFTWLFKTWLRFMSSRPPMGIVTDQCKAMQNAIQVVFPNTRHRWCLWHIMRKIAEKLKGYAQYNGIKNAMKICVYDSLLVDYFVVGWTTFIDKYGLGENDWLNTIFEERERWVPCYLKHDFWAGMTTTQTSECMNAFFDGYINARTSLSEFVKQYDNALQDKAEKEYEADFRSSSTIIPFGSNSTIERQFQAEYTHEKYMEIQVEFREKYNCYLEGVTTLEFTSTYTVLEESIVYGKPNESKYCVDFNGEDNNVTCTCLRFEFRCVLCRHSLFVLGHLRVKEVPSKLKVRHCLTN