Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027483.t1 | AES96408.1 | 92.6 | 81 | 6 | 0 | 1 | 81 | 6 | 86 | 4.40E-35 | 156.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027483.t1 | Q9SRH9 | 38.9 | 72 | 42 | 1 | 8 | 79 | 135 | 204 | 3.6e-09 | 62.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027483.t1 | G7K6X6 | 92.6 | 81 | 6 | 0 | 1 | 81 | 6 | 86 | 3.2e-35 | 156.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene027483.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027483.t1 | MTR_5g036820 | 92.593 | 81 | 6 | 0 | 1 | 81 | 6 | 86 | 7.47e-52 | 157 |
| MS.gene027483.t1 | MTR_5g036790 | 62.195 | 82 | 29 | 1 | 1 | 82 | 6 | 85 | 9.77e-32 | 106 |
| MS.gene027483.t1 | MTR_7g013525 | 40.964 | 83 | 46 | 2 | 1 | 82 | 1 | 81 | 5.65e-16 | 66.2 |
| MS.gene027483.t1 | MTR_6g008995 | 46.377 | 69 | 35 | 1 | 11 | 79 | 117 | 183 | 4.05e-14 | 65.9 |
| MS.gene027483.t1 | MTR_6g008995 | 46.377 | 69 | 35 | 1 | 11 | 79 | 60 | 126 | 4.34e-14 | 65.9 |
| MS.gene027483.t1 | MTR_6g008970 | 43.056 | 72 | 39 | 1 | 8 | 79 | 142 | 211 | 2.05e-13 | 63.9 |
| MS.gene027483.t1 | MTR_5g036670 | 42.029 | 69 | 38 | 1 | 11 | 79 | 125 | 191 | 7.09e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027483.t1 | AT3G01320 | 48.387 | 62 | 30 | 1 | 18 | 79 | 60 | 119 | 1.05e-11 | 58.9 |
| MS.gene027483.t1 | AT3G01320 | 38.889 | 72 | 42 | 1 | 8 | 79 | 135 | 204 | 2.48e-11 | 58.2 |
| MS.gene027483.t1 | AT3G01320 | 48.387 | 62 | 30 | 1 | 18 | 79 | 60 | 119 | 1.07e-11 | 58.9 |
| MS.gene027483.t1 | AT3G01320 | 38.889 | 72 | 42 | 1 | 8 | 79 | 135 | 204 | 2.44e-11 | 58.2 |
| MS.gene027483.t1 | AT5G15020 | 40.278 | 72 | 41 | 1 | 8 | 79 | 129 | 198 | 1.85e-11 | 58.5 |
| MS.gene027483.t1 | AT5G15020 | 40.278 | 72 | 41 | 1 | 8 | 79 | 129 | 198 | 1.98e-11 | 58.2 |
Find 15 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGCTATGTGCGCGAGGTGA+AGG | 0.474033 | 5.4:+26419376 | MS.gene027483:CDS |
| GGCCATGATGACCTCATTGA+TGG | 0.490730 | 5.4:+26419943 | MS.gene027483:CDS |
| TGAAAAGAATTTACGCTGAC+CGG | 0.518590 | 5.4:+26419327 | MS.gene027483:CDS |
| ACCCATCAATGAGGTCATCA+TGG | 0.540419 | 5.4:-26419945 | None:intergenic |
| TTACGCTGACCGGCCAGTGG+TGG | 0.569040 | 5.4:+26419337 | MS.gene027483:CDS |
| TGTTGGTAATGTTATTAGTG+AGG | 0.580980 | 5.4:+26419578 | MS.gene027483:CDS |
| ATAGAAAGAGAAAAGACTGT+TGG | 0.583807 | 5.4:+26419561 | MS.gene027483:CDS |
| GCCATGATGACCTCATTGAT+GGG | 0.591592 | 5.4:+26419944 | MS.gene027483:CDS |
| ACTGATAATCCACCACTGGC+CGG | 0.602964 | 5.4:-26419346 | None:intergenic |
| ATATACTGATAATCCACCAC+TGG | 0.603993 | 5.4:-26419350 | None:intergenic |
| AATTTACGCTGACCGGCCAG+TGG | 0.644697 | 5.4:+26419334 | MS.gene027483:CDS |
| ACAAATTTCATTCCTCTACG+TGG | 0.649296 | 5.4:+26419970 | MS.gene027483:CDS |
| CATATACACTAGCCACGTAG+AGG | 0.658315 | 5.4:-26419982 | None:intergenic |
| AATTTGTAAACCCATCAATG+AGG | 0.691671 | 5.4:-26419954 | None:intergenic |
| TATTTCGAGCTATGTGCGCG+AGG | 0.697429 | 5.4:+26419370 | MS.gene027483:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAAAATAATATTTTTAT+TGG | + | chr5.4:26419685-26419704 | MS.gene027483:intron | 0.0% |
| !! | TGAAAAAAAAAATATCTATT+AGG | - | chr5.4:26419470-26419489 | None:intergenic | 10.0% |
| !! | AAGAGGAAAGAAAAATAAAA+AGG | - | chr5.4:26419867-26419886 | None:intergenic | 20.0% |
| !! | TAAAAATTGTTTATGTCATG+TGG | + | chr5.4:26419765-26419784 | MS.gene027483:intron | 20.0% |
| !! | TATATGTAATTGTAGTTTAG+AGG | - | chr5.4:26419842-26419861 | None:intergenic | 20.0% |
| !!! | AATTATGATTTTACTTCTTC+AGG | + | chr5.4:26419903-26419922 | MS.gene027483:intron | 20.0% |
| !!! | TGATAGCAATAGTATTTTTA+TGG | - | chr5.4:26419811-26419830 | None:intergenic | 20.0% |
| ! | AAAAAGCAATAGTGTGAAAT+AGG | + | chr5.4:26419715-26419734 | MS.gene027483:intron | 25.0% |
| ! | AAAAGCAATAGTGTGAAATA+GGG | + | chr5.4:26419716-26419735 | MS.gene027483:intron | 25.0% |
| ! | TAAAATGAGTATTACACAAG+AGG | - | chr5.4:26419884-26419903 | None:intergenic | 25.0% |
| !! | TGTTGTTTAATTATGTCATG+TGG | + | chr5.4:26419605-26419624 | MS.gene027483:intron | 25.0% |
| AATTTGTAAACCCATCAATG+AGG | - | chr5.4:26419957-26419976 | None:intergenic | 30.0% | |
| ATAGAAAGAGAAAAGACTGT+TGG | + | chr5.4:26419561-26419580 | MS.gene027483:CDS | 30.0% | |
| GGTGAAGGTAAGTTATATTA+TGG | + | chr5.4:26419391-26419410 | MS.gene027483:intron | 30.0% | |
| TGTGGTCACAATTATAATTG+AGG | + | chr5.4:26419623-26419642 | MS.gene027483:intron | 30.0% | |
| !! | TGTTGGTAATGTTATTAGTG+AGG | + | chr5.4:26419578-26419597 | MS.gene027483:CDS | 30.0% |
| ACAAATTTCATTCCTCTACG+TGG | + | chr5.4:26419970-26419989 | MS.gene027483:CDS | 35.0% | |
| ATATACTGATAATCCACCAC+TGG | - | chr5.4:26419353-26419372 | None:intergenic | 35.0% | |
| GAGGCATAGTGAAATGATAT+TGG | + | chr5.4:26419642-26419661 | MS.gene027483:intron | 35.0% | |
| TGAAAAGAATTTACGCTGAC+CGG | + | chr5.4:26419327-26419346 | MS.gene027483:CDS | 35.0% | |
| TGAAATAGGGACAGAGAGAA+AGG | + | chr5.4:26419729-26419748 | MS.gene027483:intron | 40.0% | |
| ACCCATCAATGAGGTCATCA+TGG | - | chr5.4:26419948-26419967 | None:intergenic | 45.0% | |
| GCCATGATGACCTCATTGAT+GGG | + | chr5.4:26419944-26419963 | MS.gene027483:CDS | 45.0% | |
| ! | CAGGTTGCTGAGCTTTTTGA+AGG | + | chr5.4:26419922-26419941 | MS.gene027483:intron | 45.0% |
| ACTGATAATCCACCACTGGC+CGG | - | chr5.4:26419349-26419368 | None:intergenic | 50.0% | |
| GGCCATGATGACCTCATTGA+TGG | + | chr5.4:26419943-26419962 | MS.gene027483:CDS | 50.0% | |
| TATTTCGAGCTATGTGCGCG+AGG | + | chr5.4:26419370-26419389 | MS.gene027483:CDS | 50.0% | |
| AATTTACGCTGACCGGCCAG+TGG | + | chr5.4:26419334-26419353 | MS.gene027483:CDS | 55.0% | |
| GAGCTATGTGCGCGAGGTGA+AGG | + | chr5.4:26419376-26419395 | MS.gene027483:CDS | 60.0% | |
| TTACGCTGACCGGCCAGTGG+TGG | + | chr5.4:26419337-26419356 | MS.gene027483:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 26419326 | 26419996 | 26419326 | ID=MS.gene027483 |
| chr5.4 | mRNA | 26419326 | 26419996 | 26419326 | ID=MS.gene027483.t1;Parent=MS.gene027483 |
| chr5.4 | exon | 26419326 | 26419397 | 26419326 | ID=MS.gene027483.t1.exon1;Parent=MS.gene027483.t1 |
| chr5.4 | CDS | 26419326 | 26419397 | 26419326 | ID=cds.MS.gene027483.t1;Parent=MS.gene027483.t1 |
| chr5.4 | exon | 26419495 | 26419599 | 26419495 | ID=MS.gene027483.t1.exon2;Parent=MS.gene027483.t1 |
| chr5.4 | CDS | 26419495 | 26419599 | 26419495 | ID=cds.MS.gene027483.t1;Parent=MS.gene027483.t1 |
| chr5.4 | exon | 26419925 | 26419996 | 26419925 | ID=MS.gene027483.t1.exon3;Parent=MS.gene027483.t1 |
| chr5.4 | CDS | 26419925 | 26419996 | 26419925 | ID=cds.MS.gene027483.t1;Parent=MS.gene027483.t1 |
| Gene Sequence |
| Protein sequence |
