AlfalfaGEDB | MS.gene027485

M. sativa cultivar XinJiangDaYe / MS.gene027485

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027485.t1	AES96405.2	80	85	17	0	1	85	1	85	2.90E-29	137.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027485.t1	Q9SRH9	45.7	70	38	0	13	82	135	204	4.0e-11	68.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027485.t1	G7K6X3	80.0	85	17	0	1	85	1	85	2.1e-29	137.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene027485.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027485.t1	MTR_5g036790	80.000	85	17	0	1	85	1	85	4.17e-45	140
MS.gene027485.t1	MTR_5g036820	67.442	86	26	1	1	84	1	86	7.44e-35	114
MS.gene027485.t1	MTR_6g008970	50.000	70	35	0	13	82	142	211	6.86e-17	73.9
MS.gene027485.t1	MTR_7g013525	46.914	81	42	1	6	85	1	81	1.27e-16	68.2
MS.gene027485.t1	MTR_7g013410	45.588	68	37	0	15	82	74	141	3.71e-16	68.6
MS.gene027485.t1	MTR_6g008995	49.254	67	34	0	16	82	117	183	6.43e-16	71.2
MS.gene027485.t1	MTR_6g008995	49.254	67	34	0	16	82	60	126	7.36e-16	70.9
MS.gene027485.t1	MTR_5g036670	50.746	67	33	0	16	82	125	191	2.50e-15	69.3
MS.gene027485.t1	MTR_3g437480	72.973	37	10	0	48	84	1	37	1.64e-13	59.7
MS.gene027485.t1	MTR_8g062700	44.776	67	37	0	16	82	124	190	3.44e-13	63.5
MS.gene027485.t1	MTR_8g062700	44.776	67	37	0	16	82	124	190	3.44e-13	63.5
MS.gene027485.t1	MTR_8g062350	42.254	71	41	0	12	82	131	201	4.23e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027485.t1	AT1G10450	49.254	67	34	0	16	82	164	230	3.17e-15	69.3
MS.gene027485.t1	AT1G10450	49.254	67	34	0	16	82	113	179	3.92e-15	68.9
MS.gene027485.t1	AT1G10450	49.254	67	34	0	16	82	113	179	3.92e-15	68.9
MS.gene027485.t1	AT1G70060	50.000	72	36	0	11	82	92	163	5.54e-15	68.6
MS.gene027485.t1	AT1G70060	50.000	72	36	0	11	82	92	163	5.54e-15	68.6
MS.gene027485.t1	AT5G15020	46.479	71	38	0	12	82	128	198	6.35e-15	68.6
MS.gene027485.t1	AT5G15020	46.479	71	38	0	12	82	128	198	6.42e-15	68.6
MS.gene027485.t1	AT3G01320	45.714	70	38	0	13	82	135	204	1.46e-14	67.4
MS.gene027485.t1	AT3G01320	45.714	70	38	0	13	82	135	204	1.47e-14	67.4
MS.gene027485.t1	AT1G24190	50.746	67	33	0	16	82	96	162	1.67e-14	67.0
MS.gene027485.t1	AT1G24190	50.746	67	33	0	16	82	96	162	2.07e-14	67.0
MS.gene027485.t1	AT1G24190	50.746	67	33	0	16	82	96	162	2.11e-14	67.0
MS.gene027485.t1	AT1G70030	39.744	78	46	1	8	85	84	160	1.94e-13	62.0
MS.gene027485.t1	AT1G59890	43.284	67	38	0	16	82	125	191	1.32e-12	61.6
MS.gene027485.t1	AT1G59890	43.284	67	38	0	16	82	125	191	1.94e-12	61.2
MS.gene027485.t1	AT1G59890	43.284	67	38	0	16	82	125	191	1.98e-12	61.2
MS.gene027485.t1	AT1G59890	43.284	67	38	0	16	82	125	191	2.03e-12	61.2
MS.gene027485.t1	AT1G24230	45.312	64	34	1	21	83	169	232	6.34e-12	59.3
MS.gene027485.t1	AT1G24200	50.847	59	29	0	24	82	101	159	1.25e-11	57.8

Find 16 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGCCATGACGACCTCATTGA+TGG	0.459392	5.4:+26405569	MS.gene027485:CDS
TGAAAAGAATATACGCTAAC+CGG	0.465728	5.4:+26405101	MS.gene027485:CDS
TGTTGCGAGATATGTGTTCA+AGG	0.537764	5.4:+26405144	MS.gene027485:CDS
AAGAATATACGCTAACCGGC+CGG	0.564322	5.4:+26405105	MS.gene027485:CDS
ACCCATCAATGAGGTCGTCA+TGG	0.580750	5.4:-26405571	None:intergenic
GCCATGACGACCTCATTGAT+GGG	0.596315	5.4:+26405570	MS.gene027485:CDS
AAACGACGAATACACATGAC+GGG	0.601601	5.4:-26405294	None:intergenic
AGAAATTTCATTCCTCGACG+TGG	0.613324	5.4:+26405596	MS.gene027485:CDS
AATGATAATTCACCACCGGC+CGG	0.615597	5.4:-26405120	None:intergenic
GAGATATGTGTTCAAGGTGA+AGG	0.619178	5.4:+26405150	MS.gene027485:CDS
ACATAATGATAATTCACCAC+CGG	0.640520	5.4:-26405124	None:intergenic
GAAACGACGAATACACATGA+CGG	0.641724	5.4:-26405295	None:intergenic
AATATACGCTAACCGGCCGG+TGG	0.651380	5.4:+26405108	MS.gene027485:CDS
AATTTCTAAACCCATCAATG+AGG	0.679105	5.4:-26405580	None:intergenic
CATATACACTAGCCACGTCG+AGG	0.696720	5.4:-26405608	None:intergenic
TCTTGATGAAGTCATTCGCG+AGG	0.697148	5.4:+26405355	MS.gene027485:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGAGGAAATAAAAATAAAA+AGG	-	chr5.4:26405493-26405512	None:intergenic	15.0%
!!!	AATTATGATTTTACTTCTTC+AGG	+	chr5.4:26405529-26405548	MS.gene027485:intron	20.0%
!!!	TAAAAAGGCATATATGTAAT+TGG	-	chr5.4:26405478-26405497	None:intergenic	20.0%
!!!	TATATTCTTTTCATCTTATG+TGG	-	chr5.4:26405094-26405113	None:intergenic	20.0%
!!!	TGATAGCAATAGTATTTTTA+TGG	-	chr5.4:26405434-26405453	None:intergenic	20.0%
!	GGTGAAGGTAAATTATATTA+TGG	+	chr5.4:26405165-26405184	MS.gene027485:intron	25.0%
	AATTTCTAAACCCATCAATG+AGG	-	chr5.4:26405583-26405602	None:intergenic	30.0%
	ACATAATGATAATTCACCAC+CGG	-	chr5.4:26405127-26405146	None:intergenic	30.0%
	TAAAATGAGTATCACACAAG+AGG	-	chr5.4:26405510-26405529	None:intergenic	30.0%
	TGAAAAGAATATACGCTAAC+CGG	+	chr5.4:26405101-26405120	MS.gene027485:CDS	30.0%
	AAACGACGAATACACATGAC+GGG	-	chr5.4:26405297-26405316	None:intergenic	40.0%
	AGAAATTTCATTCCTCGACG+TGG	+	chr5.4:26405596-26405615	MS.gene027485:CDS	40.0%
	GAAACGACGAATACACATGA+CGG	-	chr5.4:26405298-26405317	None:intergenic	40.0%
	GAGATATGTGTTCAAGGTGA+AGG	+	chr5.4:26405150-26405169	MS.gene027485:CDS	40.0%
	TGTTGCGAGATATGTGTTCA+AGG	+	chr5.4:26405144-26405163	MS.gene027485:CDS	40.0%
	AAGAATATACGCTAACCGGC+CGG	+	chr5.4:26405105-26405124	MS.gene027485:CDS	45.0%
	AATGATAATTCACCACCGGC+CGG	-	chr5.4:26405123-26405142	None:intergenic	45.0%
	TCTTGATGAAGTCATTCGCG+AGG	+	chr5.4:26405355-26405374	MS.gene027485:CDS	45.0%
!	CAGGTTGCTGAGCTTTTTGA+AGG	+	chr5.4:26405548-26405567	MS.gene027485:intron	45.0%
	ACCCATCAATGAGGTCGTCA+TGG	-	chr5.4:26405574-26405593	None:intergenic	50.0%
	GCCATGACGACCTCATTGAT+GGG	+	chr5.4:26405570-26405589	MS.gene027485:CDS	50.0%
	AATATACGCTAACCGGCCGG+TGG	+	chr5.4:26405108-26405127	MS.gene027485:CDS	55.0%
	GGCCATGACGACCTCATTGA+TGG	+	chr5.4:26405569-26405588	MS.gene027485:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	26405085	26405622	26405085	ID=MS.gene027485
chr5.4	mRNA	26405085	26405622	26405085	ID=MS.gene027485.t1;Parent=MS.gene027485
chr5.4	exon	26405085	26405171	26405085	ID=MS.gene027485.t1.exon1;Parent=MS.gene027485.t1
chr5.4	CDS	26405085	26405171	26405085	ID=cds.MS.gene027485.t1;Parent=MS.gene027485.t1
chr5.4	exon	26405278	26405376	26405278	ID=MS.gene027485.t1.exon2;Parent=MS.gene027485.t1
chr5.4	CDS	26405278	26405376	26405278	ID=cds.MS.gene027485.t1;Parent=MS.gene027485.t1
chr5.4	exon	26405551	26405622	26405551	ID=MS.gene027485.t1.exon3;Parent=MS.gene027485.t1
chr5.4	CDS	26405551	26405622	26405551	ID=cds.MS.gene027485.t1;Parent=MS.gene027485.t1

Gene Sequence

>MS.gene027485

ATGAAACCACATAAGATGAAAAGAATATACGCTAACCGGCCGGTGGTGAATTATCATTATGTTGCGAGATATGTGTTCAAGGTGAAGGTAAATTATATTATGGATTCTTTCACACATATATGTTTTCAATGTAGAATTAATAATCAATGAAAATTTTCAGTTTTGATGAGTCTAATAGATCTTTTTCATGCAGACTCGATTTCAACGTGCCCGTCATGTGTATTCGTCGTTTCTAGACATACTGCGAATGTACAGAAAAAGAGAAAAAAGTCTTGATGAAGTCATTCGCGAGGTACGTGTTCTATTGTTTATGTCATGTCATGTGATCACAATTACAATTGAGATACCATAAAAATACTATTGCTATCACAATTAATGCAGCTCTAAACTCCAATTACATATATGCCTTTTTATTTTTATTTCCTCTTGTGTGATACTCATTTTAATTATGATTTTACTTCTTCAGGTTGCTGAGCTTTTTGAAGGCCATGACGACCTCATTGATGGGTTTAGAAATTTCATTCCTCGACGTGGCTAG

Protein sequence

>MS.gene027485.t1

MKPHKMKRIYANRPVVNYHYVARYVFKVKTRFQRARHVYSSFLDILRMYRKREKSLDEVIREVAELFEGHDDLIDGFRNFIPRRG