AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027499

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027499.t1 MTR_7g096610 91.111 225 18 2 1 224 1 224 1.86e-147 410
MS.gene027499.t1 MTR_1g070205 69.271 192 48 5 8 195 12 196 1.08e-88 261
MS.gene027499.t1 MTR_7g096530 71.698 106 30 0 3 108 9 114 1.72e-53 171
MS.gene027499.t1 MTR_5g080470 68.269 104 33 0 2 105 1 104 2.42e-50 162
MS.gene027499.t1 MTR_8g036085 69.903 103 31 0 8 110 23 125 2.87e-49 161
MS.gene027499.t1 MTR_8g036105 61.475 122 44 2 5 123 15 136 5.96e-48 157
MS.gene027499.t1 MTR_1g070220 68.269 104 33 0 5 108 12 115 1.95e-47 155
MS.gene027499.t1 MTR_7g074990 69.307 101 31 0 8 108 20 120 3.70e-47 155
MS.gene027499.t1 MTR_7g075230 60.465 129 42 5 5 131 9 130 1.87e-44 147
MS.gene027499.t1 MTR_6g027710 56.800 125 52 1 7 129 39 163 2.26e-44 148
MS.gene027499.t1 MTR_3g071590 44.966 149 78 3 1 149 1 145 5.74e-35 122
MS.gene027499.t1 MTR_5g083230 45.963 161 71 6 4 163 2 147 2.96e-34 120
MS.gene027499.t1 MTR_6g027700 52.727 110 49 1 8 117 7 113 5.79e-34 121
MS.gene027499.t1 MTR_4g083680 51.887 106 50 1 4 109 11 115 2.14e-32 116
MS.gene027499.t1 MTR_7g033800 55.102 98 43 1 5 102 8 104 6.62e-32 114
MS.gene027499.t1 MTR_2g068760 55.000 100 44 1 4 103 9 107 7.25e-32 114
MS.gene027499.t1 MTR_5g083010 49.541 109 53 2 5 113 8 114 7.59e-32 114
MS.gene027499.t1 MTR_5g083960 49.167 120 55 3 7 122 38 155 1.56e-31 114
MS.gene027499.t1 MTR_3g031660 42.308 156 82 5 4 158 2 150 1.59e-31 114
MS.gene027499.t1 MTR_4g105170 56.250 96 41 1 7 102 10 104 2.33e-31 114
MS.gene027499.t1 MTR_3g452660 50.000 114 56 1 2 115 1 113 2.49e-31 116
MS.gene027499.t1 MTR_3g077240 55.208 96 42 1 7 102 9 103 1.15e-30 112
MS.gene027499.t1 MTR_3g094690 50.893 112 52 2 7 117 46 155 1.88e-30 112
MS.gene027499.t1 MTR_3g071420 48.544 103 52 1 5 107 11 112 5.46e-30 110
MS.gene027499.t1 MTR_5g017950 53.922 102 46 1 1 102 1 101 6.74e-30 113
MS.gene027499.t1 MTR_6g005070 52.041 98 46 1 5 102 6 102 1.53e-29 107
MS.gene027499.t1 MTR_3g073690 48.182 110 56 1 1 110 2 110 2.05e-29 109
MS.gene027499.t1 MTR_3g094690 50.893 112 51 3 7 117 46 154 1.05e-28 108
MS.gene027499.t1 MTR_2g093310 50.515 97 47 1 7 103 32 127 1.35e-28 107
MS.gene027499.t1 MTR_6g005080 49.515 103 50 2 1 102 2 103 8.84e-28 103
MS.gene027499.t1 MTR_4g060950 52.041 98 45 2 7 103 23 119 3.83e-26 100
MS.gene027499.t1 MTR_1g095850 50.526 95 46 1 9 103 73 166 2.23e-25 99.4
MS.gene027499.t1 MTR_5g075020 54.369 103 44 2 7 108 42 142 2.78e-25 99.4
MS.gene027499.t1 MTR_5g075020 54.369 103 44 2 7 108 46 146 5.08e-25 98.6
MS.gene027499.t1 MTR_8g040900 54.023 87 39 1 2 88 1 86 3.79e-24 96.3
MS.gene027499.t1 MTR_7g028905 41.818 110 63 1 7 116 4 112 7.39e-24 92.4
MS.gene027499.t1 MTR_5g093010 43.519 108 60 1 9 116 6 112 9.63e-24 92.8
MS.gene027499.t1 MTR_8g079660 36.538 104 65 1 8 111 8 110 2.61e-17 78.6
MS.gene027499.t1 MTR_8g079620 39.423 104 62 1 8 111 8 110 4.63e-17 77.4
MS.gene027499.t1 MTR_8g017090 36.275 102 64 1 9 110 13 113 8.88e-17 77.8
MS.gene027499.t1 MTR_4g107450 36.190 105 66 1 4 108 6 109 1.93e-16 76.6
MS.gene027499.t1 MTR_2g100020 33.645 107 70 1 4 110 15 120 4.02e-16 75.5
MS.gene027499.t1 MTR_8g079580 35.294 102 64 2 8 108 8 108 1.22e-14 70.9
MS.gene027499.t1 MTR_6g011250 38.636 88 52 2 2 88 3 89 2.19e-14 69.3
MS.gene027499.t1 MTR_6g011230 34.906 106 65 2 9 111 12 116 1.21e-13 67.4
MS.gene027499.t1 MTR_6g011200 40.000 85 47 3 9 90 10 93 1.46e-13 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027499.t1 AT3G58190 80.508 118 22 1 8 124 11 128 8.25e-69 210
MS.gene027499.t1 AT2G42440 73.282 131 34 1 2 131 1 131 1.10e-66 206
MS.gene027499.t1 AT2G31310 62.500 144 50 2 2 144 1 141 1.89e-60 188
MS.gene027499.t1 AT2G42430 71.028 107 31 0 4 110 11 117 7.60e-52 168
MS.gene027499.t1 AT4G00210 60.833 120 47 0 3 122 6 125 1.03e-48 159
MS.gene027499.t1 AT5G06080 66.355 107 36 0 2 108 1 107 8.08e-48 155
MS.gene027499.t1 AT3G03760 69.608 102 31 0 7 108 50 151 1.09e-47 158
MS.gene027499.t1 AT2G45420 72.000 100 28 0 11 110 40 139 3.09e-47 157
MS.gene027499.t1 AT4G00220 68.000 100 32 0 11 110 20 119 3.05e-44 148
MS.gene027499.t1 AT2G45410 60.000 115 44 1 11 123 19 133 1.06e-42 143
MS.gene027499.t1 AT3G27650 56.250 96 41 1 7 102 38 132 9.94e-32 114
MS.gene027499.t1 AT2G30130 50.000 118 57 2 2 118 1 117 1.65e-31 114
MS.gene027499.t1 AT5G63090 56.250 96 41 1 7 102 10 104 6.07e-31 112
MS.gene027499.t1 AT5G63090 56.250 96 41 1 7 102 10 104 6.07e-31 112
MS.gene027499.t1 AT5G63090 56.250 96 41 1 7 102 10 104 6.07e-31 112
MS.gene027499.t1 AT5G63090 56.250 96 41 1 7 102 10 104 6.07e-31 112
MS.gene027499.t1 AT3G11090 44.531 128 66 3 5 128 8 134 8.34e-30 109
MS.gene027499.t1 AT1G07900 52.577 97 45 1 7 103 32 127 1.60e-29 109
MS.gene027499.t1 AT1G31320 52.174 92 43 1 4 95 9 99 2.23e-29 108
MS.gene027499.t1 AT1G16530 54.545 99 43 2 7 104 13 110 2.99e-29 107
MS.gene027499.t1 AT2G28500 52.577 97 45 1 7 103 54 149 9.94e-29 108
MS.gene027499.t1 AT5G66870 50.459 109 53 1 2 110 1 108 4.99e-28 108
MS.gene027499.t1 AT2G30340 50.485 103 48 2 7 108 52 152 7.25e-28 107
MS.gene027499.t1 AT2G30340 50.485 103 48 2 7 108 51 151 7.97e-28 106
MS.gene027499.t1 AT2G23660 48.039 102 52 1 7 108 4 104 9.99e-26 102
MS.gene027499.t1 AT2G23660 48.039 102 52 1 7 108 4 104 9.99e-26 102
MS.gene027499.t1 AT2G23660 48.039 102 52 1 7 108 4 104 9.99e-26 102
MS.gene027499.t1 AT3G26660 46.316 95 50 1 9 103 6 99 1.36e-25 97.1
MS.gene027499.t1 AT3G26620 46.316 95 50 1 9 103 6 99 2.67e-25 96.3
MS.gene027499.t1 AT1G65620 58.537 82 33 1 7 88 8 88 1.11e-24 97.1
MS.gene027499.t1 AT1G65620 58.537 82 33 1 7 88 8 88 1.11e-24 97.1
MS.gene027499.t1 AT1G65620 58.537 82 33 1 7 88 8 88 1.11e-24 97.1
MS.gene027499.t1 AT1G65620 58.537 82 33 1 7 88 8 88 1.11e-24 97.1
MS.gene027499.t1 AT1G65620 58.537 82 33 1 7 88 8 88 1.11e-24 97.1
MS.gene027499.t1 AT2G40470 53.398 103 45 2 7 108 19 119 2.22e-24 96.3
MS.gene027499.t1 AT2G40470 53.398 103 45 2 7 108 44 144 3.24e-24 96.3
MS.gene027499.t1 AT3G50510 41.593 113 65 1 2 114 6 117 5.16e-24 95.1
MS.gene027499.t1 AT3G50510 41.593 113 65 1 2 114 6 117 5.16e-24 95.1
MS.gene027499.t1 AT3G13850 38.739 111 58 3 9 111 37 145 2.80e-17 78.6
MS.gene027499.t1 AT1G72980 36.190 105 66 1 4 108 9 112 9.62e-16 73.6
MS.gene027499.t1 AT5G35900 38.739 111 66 2 9 118 6 115 9.67e-16 73.2
MS.gene027499.t1 AT1G06280 39.474 76 45 1 9 84 25 99 8.93e-14 68.2
MS.gene027499.t1 AT3G47870 34.831 89 57 1 4 92 32 119 3.75e-13 67.8

Find 45 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACTTCAAGATCCAATTTA+TGG 0.150762 5.4:-26001759 MS.gene027499:CDS
CCTTATGAATAGCTGCAAAA+TGG 0.260543 5.4:+26001877 None:intergenic
ATTAGTGGAAGAAGAGAAAT+CGG 0.262535 5.4:+26001028 None:intergenic
TTGAGGAGGAAATGTGTTAA+AGG 0.286061 5.4:-26001942 MS.gene027499:CDS
GCAGCTATTCATAAGGTCTT+TGG 0.287235 5.4:-26001870 MS.gene027499:CDS
TTTCAAAATTCTTCTCATTA+GGG 0.291892 5.4:+26001129 None:intergenic
TGGGCAACAGAACCATAAAT+TGG 0.301569 5.4:+26001747 None:intergenic
CTGTCTTTGCATGTTGTCAT+AGG 0.336728 5.4:+26000857 None:intergenic
CTCATGGATCTAAACCCTAT+TGG 0.344271 5.4:-26000963 MS.gene027499:CDS
ATTGGAAACTATGAAAATTC+AGG 0.345302 5.4:-26000945 MS.gene027499:CDS
TTTCCTCCTCAAGAATTTGC+AGG 0.357584 5.4:+26001953 None:intergenic
ATGAGATGCTTTCTTCCAAT+AGG 0.363922 5.4:+26000919 None:intergenic
TGAAAATTCAGGAACCCTAT+TGG 0.376897 5.4:-26000934 MS.gene027499:CDS
AATGGGTAGCACTTTGTTCA+TGG 0.389795 5.4:+26001895 None:intergenic
ACGGCCATGATGACTGGTTC+TGG 0.395558 5.4:-26001990 None:intergenic
TGAGATGCTTTCTTCCAATA+GGG 0.428947 5.4:+26000920 None:intergenic
TCTTGAGGGAGAATATTAGT+AGG 0.429657 5.4:+26001104 None:intergenic
CTTATGAATAGCTGCAAAAT+GGG 0.434815 5.4:+26001878 None:intergenic
ATTCTTACGGCCATGATGAC+TGG 0.446907 5.4:-26001996 None:intergenic
TGTTGTTGATGAGAGATTAG+TGG 0.463491 5.4:+26001013 None:intergenic
TCCCTCAAGATCTTCAAAGT+TGG 0.463884 5.4:-26001091 MS.gene027499:CDS
CTTTGTTCATGGCAAAAGTA+AGG 0.479437 5.4:+26001906 None:intergenic
CAACAACACAATATTACAAT+GGG 0.480559 5.4:-26000998 MS.gene027499:CDS
CAAATTAGCCACTTACTGAT+AGG 0.482945 5.4:+26000830 None:intergenic
CAGATGAACAAACCTGTTGT+TGG 0.487101 5.4:+26001712 None:intergenic
ACTGGTTCTGGTTCTCCTTG+TGG 0.494488 5.4:-26001978 MS.gene027499:CDS
TCTTCAAAGTTGGTTCCAGA+TGG 0.523638 5.4:-26001081 MS.gene027499:CDS
AACCAACTTTGAAGATCTTG+AGG 0.528680 5.4:+26001089 None:intergenic
GCTTCACAACGATCAGTCAC+AGG 0.531664 5.4:+26001813 None:intergenic
TGGGACTACAAACACTCTCA+TGG 0.531675 5.4:-26000979 MS.gene027499:CDS
TTAGTGGAAGAAGAGAAATC+GGG 0.531706 5.4:+26001029 None:intergenic
ACCAACTTTGAAGATCTTGA+GGG 0.534056 5.4:+26001090 None:intergenic
GTGGAGCCTGCAAATTCTTG+AGG 0.534107 5.4:-26001959 MS.gene027499:CDS
AAGAAATTGAGAACACATGC+TGG 0.541655 5.4:+26001052 None:intergenic
ATGAACAAACCTGTTGTTGG+AGG 0.559046 5.4:+26001715 None:intergenic
AGAACCAGAACCAGTCATCA+TGG 0.567393 5.4:+26001986 None:intergenic
TCAACAACACAATATTACAA+TGG 0.569747 5.4:-26000999 MS.gene027499:CDS
TGAGAGAACAAGCAGCTCAA+AGG 0.584503 5.4:-26001181 MS.gene027499:CDS
AAGAATTTGCAGGCTCCACA+AGG 0.586151 5.4:+26001963 None:intergenic
CAGTCACAGGGAGGTGTGCG+AGG 0.596818 5.4:+26001826 None:intergenic
TGAACAAACCTGTTGTTGGA+GGG 0.615563 5.4:+26001716 None:intergenic
GAGCCTGCAAATTCTTGAGG+AGG 0.637179 5.4:-26001956 MS.gene027499:CDS
CTTCACAACGATCAGTCACA+GGG 0.652040 5.4:+26001814 None:intergenic
ATGACAACATGCAAAGACAG+TGG 0.691269 5.4:-26000854 MS.gene027499:CDS
CACAACGATCAGTCACAGGG+AGG 0.754403 5.4:+26001817 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTCTTTTTTATATATAAAC+TGG + chr5.4:26001288-26001307 None:intergenic 15.0%
!!! TTGAGTTTGTAATAAAATTT+TGG - chr5.4:26001500-26001519 MS.gene027499:intron 15.0%
!! TTTCAAAATTCTTCTCATTA+GGG + chr5.4:26001700-26001719 None:intergenic 20.0%
!!! AATTTTCATAGTTTCCAATA+GGG + chr5.4:26001880-26001899 None:intergenic 20.0%
!!! ATTAATTAACAAGAGTATCA+AGG - chr5.4:26001237-26001256 MS.gene027499:intron 20.0%
!!! TTAATTAACAAGAGTATCAA+GGG - chr5.4:26001238-26001257 MS.gene027499:intron 20.0%
!!! TTTTCAAAATTCTTCTCATT+AGG + chr5.4:26001701-26001720 None:intergenic 20.0%
! ATAGTAAGACCTAATAAACA+CGG - chr5.4:26001328-26001347 MS.gene027499:intron 25.0%
! ATTGGAAACTATGAAAATTC+AGG - chr5.4:26001881-26001900 MS.gene027499:CDS 25.0%
! CAACAACACAATATTACAAT+GGG - chr5.4:26001828-26001847 MS.gene027499:CDS 25.0%
! TCAACAACACAATATTACAA+TGG - chr5.4:26001827-26001846 MS.gene027499:CDS 25.0%
!! GAATTTTCATAGTTTCCAAT+AGG + chr5.4:26001881-26001900 None:intergenic 25.0%
!!! AGGTCTTACTATTGATTTTT+TGG + chr5.4:26001320-26001339 None:intergenic 25.0%
AGACTTCAAGATCCAATTTA+TGG - chr5.4:26001067-26001086 MS.gene027499:CDS 30.0%
ATTAGTGGAAGAAGAGAAAT+CGG + chr5.4:26001801-26001820 None:intergenic 30.0%
CTTATGAATAGCTGCAAAAT+GGG + chr5.4:26000951-26000970 None:intergenic 30.0%
! ATTAGGGTTTTCAGAATTTG+GGG + chr5.4:26001684-26001703 None:intergenic 30.0%
! CATTAGGGTTTTCAGAATTT+GGG + chr5.4:26001685-26001704 None:intergenic 30.0%
! TATATTTGACAAAAGCACGA+TGG - chr5.4:26001392-26001411 MS.gene027499:intron 30.0%
! TCATTAGGGTTTTCAGAATT+TGG + chr5.4:26001686-26001705 None:intergenic 30.0%
AAGAAATTGAGAACACATGC+TGG + chr5.4:26001777-26001796 None:intergenic 35.0%
AATGTAAGCGTATACCATCT+AGG + chr5.4:26001161-26001180 None:intergenic 35.0%
ATGAGATGCTTTCTTCCAAT+AGG + chr5.4:26001910-26001929 None:intergenic 35.0%
ATGTAAGCGTATACCATCTA+GGG + chr5.4:26001160-26001179 None:intergenic 35.0%
CCTTATGAATAGCTGCAAAA+TGG + chr5.4:26000952-26000971 None:intergenic 35.0%
CTTAAACTGAAAACCCTAGA+TGG - chr5.4:26001144-26001163 MS.gene027499:CDS 35.0%
CTTTGTTCATGGCAAAAGTA+AGG + chr5.4:26000923-26000942 None:intergenic 35.0%
GGGTACTCCAGTTTATATAA+GGG - chr5.4:26001258-26001277 MS.gene027499:intron 35.0%
TCTTGAGGGAGAATATTAGT+AGG + chr5.4:26001725-26001744 None:intergenic 35.0%
TGAAAATTCAGGAACCCTAT+TGG - chr5.4:26001892-26001911 MS.gene027499:CDS 35.0%
TGAGATGCTTTCTTCCAATA+GGG + chr5.4:26001909-26001928 None:intergenic 35.0%
TGTAAGACTTGGCACATATT+TGG - chr5.4:26001359-26001378 MS.gene027499:intron 35.0%
TTAGTGGAAGAAGAGAAATC+GGG + chr5.4:26001800-26001819 None:intergenic 35.0%
TTCTTCTTCCTGATCTTTGT+AGG - chr5.4:26001592-26001611 MS.gene027499:intron 35.0%
TTGAGGAGGAAATGTGTTAA+AGG - chr5.4:26000884-26000903 MS.gene027499:CDS 35.0%
TTGGTGTATTATTGATGCAC+TGG - chr5.4:26001476-26001495 MS.gene027499:intron 35.0%
TTGTTGGAGGGCAAAAATAT+GGG + chr5.4:26001101-26001120 None:intergenic 35.0%
! AACCAACTTTGAAGATCTTG+AGG + chr5.4:26001740-26001759 None:intergenic 35.0%
! ACCAACTTTGAAGATCTTGA+GGG + chr5.4:26001739-26001758 None:intergenic 35.0%
! AGGGTACTCCAGTTTATATA+AGG - chr5.4:26001257-26001276 MS.gene027499:intron 35.0%
! CCATTTTGCAGCTATTCATA+AGG - chr5.4:26000949-26000968 MS.gene027499:CDS 35.0%
! TGGAGTACCCTTATATAAAC+TGG + chr5.4:26001268-26001287 None:intergenic 35.0%
! TGTTGTTGATGAGAGATTAG+TGG + chr5.4:26001816-26001835 None:intergenic 35.0%
AATGGGTAGCACTTTGTTCA+TGG + chr5.4:26000934-26000953 None:intergenic 40.0%
ACTCGCGCAGTAAATACAAT+TGG - chr5.4:26001457-26001476 MS.gene027499:intron 40.0%
AGATCGACACCGTGTTTATT+AGG + chr5.4:26001340-26001359 None:intergenic 40.0%
ATGAACAAACCTGTTGTTGG+AGG + chr5.4:26001114-26001133 None:intergenic 40.0%
ATGACAACATGCAAAGACAG+TGG - chr5.4:26001972-26001991 MS.gene027499:CDS 40.0%
ATTGACCACCTACAAAGATC+AGG + chr5.4:26001603-26001622 None:intergenic 40.0%
CAGATGAACAAACCTGTTGT+TGG + chr5.4:26001117-26001136 None:intergenic 40.0%
CTCATGGATCTAAACCCTAT+TGG - chr5.4:26001863-26001882 MS.gene027499:CDS 40.0%
CTGTCTTTGCATGTTGTCAT+AGG + chr5.4:26001972-26001991 None:intergenic 40.0%
GCAGCTATTCATAAGGTCTT+TGG - chr5.4:26000956-26000975 MS.gene027499:CDS 40.0%
GTTGTTGGAGGGCAAAAATA+TGG + chr5.4:26001102-26001121 None:intergenic 40.0%
TCCCTCAAGATCTTCAAAGT+TGG - chr5.4:26001735-26001754 MS.gene027499:CDS 40.0%
TGAACAAACCTGTTGTTGGA+GGG + chr5.4:26001113-26001132 None:intergenic 40.0%
TGGGCAACAGAACCATAAAT+TGG + chr5.4:26001082-26001101 None:intergenic 40.0%
TTCTTCCTGATCTTTGTAGG+TGG - chr5.4:26001595-26001614 MS.gene027499:intron 40.0%
TTGTATTTACTGCGCGAGTT+TGG + chr5.4:26001456-26001475 None:intergenic 40.0%
TTTCCTCCTCAAGAATTTGC+AGG + chr5.4:26000876-26000895 None:intergenic 40.0%
! ACATGCTGGAATTTTCCATC+TGG + chr5.4:26001763-26001782 None:intergenic 40.0%
! TCTTCAAAGTTGGTTCCAGA+TGG - chr5.4:26001745-26001764 MS.gene027499:CDS 40.0%
!! TATTTTTGCCCTCCAACAAC+AGG - chr5.4:26001102-26001121 MS.gene027499:CDS 40.0%
AAGAATTTGCAGGCTCCACA+AGG + chr5.4:26000866-26000885 None:intergenic 45.0%
CTTCACAACGATCAGTCACA+GGG + chr5.4:26001015-26001034 None:intergenic 45.0%
TGAGAGAACAAGCAGCTCAA+AGG - chr5.4:26001645-26001664 MS.gene027499:intron 45.0%
TGGGACTACAAACACTCTCA+TGG - chr5.4:26001847-26001866 MS.gene027499:CDS 45.0%
! ACAAAAGCACGATGGCATCA+AGG - chr5.4:26001400-26001419 MS.gene027499:intron 45.0%
! CACTGTGTTGATCTATGCAG+TGG - chr5.4:26001547-26001566 MS.gene027499:intron 45.0%
GAGCCTGCAAATTCTTGAGG+AGG - chr5.4:26000870-26000889 MS.gene027499:CDS 50.0%
GCTTCACAACGATCAGTCAC+AGG + chr5.4:26001016-26001035 None:intergenic 50.0%
GTGGAGCCTGCAAATTCTTG+AGG - chr5.4:26000867-26000886 MS.gene027499:CDS 50.0%
!! ACTGGTTCTGGTTCTCCTTG+TGG - chr5.4:26000848-26000867 MS.gene027499:CDS 50.0%
!! CGGTGTCGATCTGTAAGACT+TGG - chr5.4:26001348-26001367 MS.gene027499:intron 50.0%
CACAACGATCAGTCACAGGG+AGG + chr5.4:26001012-26001031 None:intergenic 55.0%
! CAGTCACAGGGAGGTGTGCG+AGG + chr5.4:26001003-26001022 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 26000842 26002006 26000842 ID=MS.gene027499
chr5.4 mRNA 26000842 26002006 26000842 ID=MS.gene027499.t1;Parent=MS.gene027499
chr5.4 exon 26001725 26002006 26001725 ID=MS.gene027499.t1.exon1;Parent=MS.gene027499.t1
chr5.4 CDS 26001725 26002006 26001725 ID=cds.MS.gene027499.t1;Parent=MS.gene027499.t1
chr5.4 exon 26000842 26001234 26000842 ID=MS.gene027499.t1.exon2;Parent=MS.gene027499.t1
chr5.4 CDS 26000842 26001234 26000842 ID=cds.MS.gene027499.t1;Parent=MS.gene027499.t1
Gene Sequence

>MS.gene027499

ATGATGACTGGTTCTGGTTCTCCTTGTGGAGCCTGCAAATTCTTGAGGAGGAAATGTGTTAAAGGTTGTGTTTTTGCACCTTACTTTTGCCATGAACAAAGTGCTACCCATTTTGCAGCTATTCATAAGGTCTTTGGTGCAAGTAATGTTTCAAAGCTCCTCGCACACCTCCCTGTGACTGATCGTTGTGAAGCTGCAGTCACAATCTCTTATGAAGCTCAAGCTAGACTTCAAGATCCAATTTATGGTTCTGTTGCCCATATTTTTGCCCTCCAACAACAGGTTTGTTCATCTGTCGTGATCTTAAACTGAAAACCCTAGATGGTATACGCTTACATTTTTTTTCTTCATAATTGCAAAGAAGAATTTAAATTAATTAATTAATTAATTAATTAATTAATTAACAAGAGTATCAAGGGTACTCCAGTTTATATAAGGGTACTCCAGTTTATATATAAAAAAGAGTCTACAACGACCAAAAAATCAATAGTAAGACCTAATAAACACGGTGTCGATCTGTAAGACTTGGCACATATTTGGACTGTATAAATATATTTGACAAAAGCACGATGGCATCAAGGTTTTGTTTAAGATTCAAAGTGTAGCTTTTGCCAAACTCGCGCAGTAAATACAATTGGTGTATTATTGATGCACTGGTTTGAGTTTGTAATAAAATTTTGGCTATTAAAGAAAAAAATACATAAGCACTGTGTTGATCTATGCAGTGGTCAATTTACTAATAAATGCATTTTCTTCTTCCTGATCTTTGTAGGTGGTCAATTTGCAAGCACAGCTAGCTTTTCTGAGAGAACAAGCAGCTCAAAGGTGTCTCAATAATGCCCCAAATTCTGAAAACCCTAATGAGAAGAATTTTGAAAAACCTACTAATATTCTCCCTCAAGATCTTCAAAGTTGGTTCCAGATGGAAAATTCCAGCATGTGTTCTCAATTTCTTCCCGATTTCTCTTCTTCCACTAATCTCTCATCAACAACACAATATTACAATGGGACTACAAACACTCTCATGGATCTAAACCCTATTGGAAACTATGAAAATTCAGGAACCCTATTGGAAGAAAGCATCTCATCATTTTCTACTTTTGATGAGCGACGTAATAATTCAATGTCCTATGACAACATGCAAAGACAGTGGACCTATCAGTAA
Protein sequence

>MS.gene027499.t1

MMTGSGSPCGACKFLRRKCVKGCVFAPYFCHEQSATHFAAIHKVFGASNVSKLLAHLPVTDRCEAAVTISYEAQARLQDPIYGSVAHIFALQQQVVNLQAQLAFLREQAAQRCLNNAPNSENPNEKNFEKPTNILPQDLQSWFQMENSSMCSQFLPDFSSSTNLSSTTQYYNGTTNTLMDLNPIGNYENSGTLLEESISSFSTFDERRNNSMSYDNMQRQWTYQ