AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027575

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027575.t1 MTR_5g035040 80.612 392 43 3 12 394 9 376 0.0 599
MS.gene027575.t1 MTR_5g035060 64.214 299 58 6 145 394 1 299 7.27e-113 332
MS.gene027575.t1 MTR_5g035050 86.364 88 12 0 307 394 29 116 2.91e-47 157
MS.gene027575.t1 MTR_7g033775 70.370 135 32 3 5 132 15 148 4.47e-38 136
MS.gene027575.t1 MTR_4g105200 51.807 83 35 1 35 112 36 118 2.55e-19 85.9
MS.gene027575.t1 MTR_1g090250 38.000 100 57 1 34 128 27 126 2.45e-14 70.5
MS.gene027575.t1 MTR_3g109190 35.135 111 66 2 27 132 24 133 3.57e-14 70.1
MS.gene027575.t1 MTR_6g005040 39.370 127 70 2 18 137 41 167 1.41e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027575.t1 AT1G24040 60.090 223 82 2 167 382 80 302 3.68e-89 272
MS.gene027575.t1 AT1G24040 60.090 223 82 2 167 382 80 302 3.68e-89 272
MS.gene027575.t1 AT1G24040 60.090 223 82 2 167 382 80 302 3.68e-89 272
MS.gene027575.t1 AT3G01570 42.857 133 69 3 8 135 7 137 1.06e-25 102
MS.gene027575.t1 AT3G27660 48.696 115 51 2 31 137 43 157 1.16e-16 78.2
MS.gene027575.t1 AT3G27660 48.696 115 51 2 31 137 43 157 1.16e-16 78.2
MS.gene027575.t1 AT5G51210 40.909 88 47 1 30 112 17 104 2.59e-15 72.8
MS.gene027575.t1 AT5G40420 50.962 104 46 1 34 132 48 151 6.57e-15 73.2
MS.gene027575.t1 AT4G25140 38.776 98 50 2 19 111 27 119 6.55e-12 63.9

Find 91 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCAAATCCGGTTCAATTT+TGG 0.113243 5.1:-25823122 MS.gene027575:CDS
CTTGCTGGACTCTCCCTTCT+TGG 0.217986 5.1:-25823526 MS.gene027575:CDS
CCTGTAGAGGCCCTTGATCC+TGG 0.232255 5.1:+25823353 None:intergenic
ACATGTTGATATAACATTTC+GGG 0.273357 5.1:+25823023 None:intergenic
AACCAAAATTGAACCGGATT+TGG 0.284192 5.1:+25823120 None:intergenic
TTGAACCGGATTTGGTGTTT+TGG 0.312879 5.1:+25823128 None:intergenic
AACATGTTGATATAACATTT+CGG 0.331289 5.1:+25823022 None:intergenic
CAAGTGCTTGCCGTAATTAC+CGG 0.339584 5.1:-25823577 MS.gene027575:CDS
TAATCAAATATTGTTTCATA+AGG 0.361459 5.1:+25822993 None:intergenic
CCGCATGTGGCGACACTGAT+TGG 0.372849 5.1:-25822953 MS.gene027575:CDS
TCTTGGAACACTCACTGGAC+TGG 0.377988 5.1:-25823509 MS.gene027575:CDS
TTCGTCTCTGCAAAGTCAAC+AGG 0.380774 5.1:+25821872 None:intergenic
GAGGACTCGACGAGTGGTTT+TGG 0.383378 5.1:+25823201 None:intergenic
CTTGGAACACTCACTGGACT+GGG 0.386557 5.1:-25823508 MS.gene027575:CDS
TGGCACTGTCCTTGTTCTCA+TGG 0.386698 5.1:-25823393 MS.gene027575:CDS
GGAAGGATTCGAGGTGTTGC+AGG 0.387564 5.1:+25823158 None:intergenic
GGTGTACTGCTTGCACTTGC+TGG 0.398295 5.1:-25823541 MS.gene027575:CDS
GTGTTGCAGGTTGGTTAGTT+CGG 0.403050 5.1:+25823171 None:intergenic
ATGGTGGCTGGAACAAGAAC+AGG 0.405324 5.1:+25823454 None:intergenic
TACTCGAACCAAAATTGAAC+CGG 0.409292 5.1:+25823114 None:intergenic
TACAGGAAGGCCGGTAATTA+CGG 0.409924 5.1:+25823567 None:intergenic
TCTGCGAGTAAACAGACTAT+TGG 0.411736 5.1:+25823070 None:intergenic
CTGTAGAGGCCCTTGATCCT+GGG 0.419299 5.1:+25823354 None:intergenic
CCTTAGGCCCTCAAAATCAT+TGG 0.420597 5.1:-25823234 MS.gene027575:CDS
TTCGCCTTACATTTATAGCT+TGG 0.446373 5.1:-25822816 MS.gene027575:CDS
ATAAATGTAAGGCGAATCCT+TGG 0.447429 5.1:+25822823 None:intergenic
TCCCTTCTTGGAACACTCAC+TGG 0.449866 5.1:-25823514 MS.gene027575:CDS
ATTCTTTCACTCTCCTCCTT+AGG 0.453455 5.1:-25823250 MS.gene027575:CDS
CTGCGAGTAAACAGACTATT+GGG 0.455560 5.1:+25823071 None:intergenic
GGAAGTAGAGGAAGGATTCG+AGG 0.455777 5.1:+25823149 None:intergenic
CGCATGTGGCGACACTGATT+GGG 0.458513 5.1:-25822952 MS.gene027575:CDS
ATTCCCTTCAGGAGGGGCAT+TGG 0.469054 5.1:-25822045 MS.gene027575:intron
TACTTCCAAAACACCAAATC+CGG 0.469207 5.1:-25823133 MS.gene027575:CDS
CAAGTGAGGAACTGATTTCT+CGG 0.471299 5.1:-25822005 MS.gene027575:CDS
CCGTGGATAAATCTCTTAGA+AGG 0.481490 5.1:-25822793 MS.gene027575:intron
GGCACTGTCCTTGTTCTCAT+GGG 0.495063 5.1:-25823392 MS.gene027575:CDS
GCAAGCACTTGAGAAGTTGA+TGG 0.495981 5.1:+25823589 None:intergenic
CCAGGATCAAGGGCCTCTAC+AGG 0.506135 5.1:-25823353 MS.gene027575:CDS
CATTGGACGGCATCTTCTAA+AGG 0.513599 5.1:-25822028 MS.gene027575:CDS
TCCAGTGAGTGTTCCAAGAA+GGG 0.516170 5.1:+25823513 None:intergenic
AATTACATACCCCAGGATCA+AGG 0.516525 5.1:-25823364 MS.gene027575:CDS
TTTCTTCAGTTCTATTACCA+TGG 0.524414 5.1:-25823278 MS.gene027575:CDS
ACGGCCAAGCTATAAATGTA+AGG 0.524650 5.1:+25822812 None:intergenic
GTCCAGTGAGTGTTCCAAGA+AGG 0.526230 5.1:+25823512 None:intergenic
CCTCAACATCGTTATGAAGT+TGG 0.529388 5.1:-25823646 MS.gene027575:CDS
TGCTTCGCCCACTCCTCCCA+AGG 0.530054 5.1:-25822840 MS.gene027575:CDS
GGCTGAAGAGGATGAAAAGT+GGG 0.530470 5.1:+25823476 None:intergenic
GGCGAATCCTTGGGAGGAGT+GGG 0.531199 5.1:+25822833 None:intergenic
GAACAAGAACAGGGCTGAAG+AGG 0.534164 5.1:+25823464 None:intergenic
GCAAGCAGTACACCGCCTAC+AGG 0.537513 5.1:+25823550 None:intergenic
TTACATTTATAGCTTGGCCG+TGG 0.542063 5.1:-25822810 MS.gene027575:CDS
TCTACAGGAGCAACTCGAGT+TGG 0.549387 5.1:-25823338 MS.gene027575:CDS
CAACTTCATAACGATGTTGA+GGG 0.550235 5.1:+25823647 None:intergenic
AGGCGAATCCTTGGGAGGAG+TGG 0.550564 5.1:+25822832 None:intergenic
AAGACAGCGACAATGGTGGC+TGG 0.555711 5.1:+25823442 None:intergenic
GGTGAAGAAATGCAGTTGGC+TGG 0.557318 5.1:-25822908 MS.gene027575:CDS
TTTCTTCTACTCATAGTCAT+CGG 0.564020 5.1:+25821786 None:intergenic
CCTTCAGGAGGGGCATTGGA+CGG 0.564318 5.1:-25822041 MS.gene027575:intron
AACTCGAGTTGCTCCTGTAG+AGG 0.565489 5.1:+25823340 None:intergenic
CAACATCGTTATGAAGTTGG+TGG 0.571137 5.1:-25823643 MS.gene027575:CDS
TTTGAAGGCGTAGTTATGAG+AGG 0.572341 5.1:+25821826 None:intergenic
TAAATGTAAGGCGAATCCTT+GGG 0.579236 5.1:+25822824 None:intergenic
GTTTGTGAGGACTCGACGAG+TGG 0.581088 5.1:+25823195 None:intergenic
TGTAGAGGCCCTTGATCCTG+GGG 0.582357 5.1:+25823355 None:intergenic
TGGTTAGTTCGGAGTTTGTG+AGG 0.583118 5.1:+25823182 None:intergenic
ATTACATACCCCAGGATCAA+GGG 0.590997 5.1:-25823363 MS.gene027575:CDS
GGATTCGAGGTGTTGCAGGT+TGG 0.596620 5.1:+25823162 None:intergenic
CCAACTTCATAACGATGTTG+AGG 0.597401 5.1:+25823646 None:intergenic
GAGTGAAAGAATTGCTGCCA+TGG 0.598782 5.1:+25823261 None:intergenic
AACAATATTTGATTAAACGA+AGG 0.599669 5.1:-25822985 MS.gene027575:CDS
TGGAACACTCACTGGACTGG+GGG 0.600169 5.1:-25823506 MS.gene027575:CDS
TACTCATAGTCATCGGAACA+TGG 0.601957 5.1:+25821793 None:intergenic
ATTCATGACCCATGAGAACA+AGG 0.605807 5.1:+25823384 None:intergenic
GGTCATGAATTACATACCCC+AGG 0.611661 5.1:-25823371 MS.gene027575:CDS
GTAATTACCGGCCTTCCTGT+AGG 0.612160 5.1:-25823565 MS.gene027575:CDS
TACACCGCCTACAGGAAGGC+CGG 0.615441 5.1:+25823558 None:intergenic
GGGCTGAAGAGGATGAAAAG+TGG 0.618573 5.1:+25823475 None:intergenic
CCAATCAGTGTCGCCACATG+CGG 0.627529 5.1:+25822953 None:intergenic
AAGGACGTTGATGCCGCATG+TGG 0.632685 5.1:-25822966 MS.gene027575:CDS
GCAGTACACCGCCTACAGGA+AGG 0.642229 5.1:+25823554 None:intergenic
CCTTCTAAGAGATTTATCCA+CGG 0.645724 5.1:+25822793 None:intergenic
TTGGAACACTCACTGGACTG+GGG 0.646592 5.1:-25823507 MS.gene027575:CDS
ACGATGTTGAGGGGTAACAC+TGG 0.650096 5.1:+25823657 None:intergenic
TGATGGTGAAGAAATGCAGT+TGG 0.650400 5.1:-25822912 MS.gene027575:CDS
GCATCTTCTAAAGGCAAGTG+AGG 0.653743 5.1:-25822019 MS.gene027575:CDS
ATGTAAGGCGAATCCTTGGG+AGG 0.670081 5.1:+25822827 None:intergenic
AACTTCATAACGATGTTGAG+GGG 0.671058 5.1:+25823648 None:intergenic
TGGTGGCTGGAACAAGAACA+GGG 0.708517 5.1:+25823455 None:intergenic
ATTACCGGCCTTCCTGTAGG+CGG 0.711293 5.1:-25823562 MS.gene027575:CDS
CTGGTATCGTAACAGCGTGG+TGG 0.712087 5.1:+25823676 None:intergenic
ACACTGGTATCGTAACAGCG+TGG 0.804350 5.1:+25823673 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATCAAATATTGTTTCATA+AGG + chr5.1:25822493-25822512 None:intergenic 15.0%
!! AACAATATTTGATTAAACGA+AGG - chr5.1:25822498-25822517 MS.gene027575:intron 20.0%
!! AACATGTTGATATAACATTT+CGG + chr5.1:25822464-25822483 None:intergenic 20.0%
!! AGCTTAATTATTTAAATTGC+AGG + chr5.1:25823263-25823282 None:intergenic 20.0%
!! TTAGAAAGTTAAAACAGAAT+CGG + chr5.1:25823398-25823417 None:intergenic 20.0%
!! TTTCGATTATTCAAAGATTA+AGG - chr5.1:25822870-25822889 MS.gene027575:CDS 20.0%
!!! GAGATTTGTTTTGATAAAAA+TGG - chr5.1:25822605-25822624 MS.gene027575:intron 20.0%
! AATAAGCTAAAATCAGCTTA+TGG + chr5.1:25822999-25823018 None:intergenic 25.0%
! ACATGTTGATATAACATTTC+GGG + chr5.1:25822463-25822482 None:intergenic 25.0%
! ATAAGAACTAATTTGACCAA+GGG - chr5.1:25822787-25822806 MS.gene027575:intron 25.0%
! ATTAAGGCTGTATTTAGATT+GGG - chr5.1:25822886-25822905 MS.gene027575:CDS 25.0%
! GGTTACTTAAATCTTAAACT+TGG + chr5.1:25823287-25823306 None:intergenic 25.0%
! TATTCTTGGTCTAATTTGAT+TGG - chr5.1:25822816-25822835 MS.gene027575:CDS 25.0%
! TGTTTGAAAATATGAGCAAT+GGG + chr5.1:25823335-25823354 None:intergenic 25.0%
!! GCTTTTGTATACATGTTAAA+TGG + chr5.1:25823555-25823574 None:intergenic 25.0%
AAATGCAAATTTCAGCAACT+TGG + chr5.1:25822735-25822754 None:intergenic 30.0%
AATATGAGCAATGGGAATTT+CGG + chr5.1:25823327-25823346 None:intergenic 30.0%
ATATTTAATTCCCTTCAGGA+GGG - chr5.1:25823431-25823450 MS.gene027575:CDS 30.0%
ATTGGCTATATACATATCCA+GGG - chr5.1:25822834-25822853 MS.gene027575:CDS 30.0%
CATAAGAACTAATTTGACCA+AGG - chr5.1:25822786-25822805 MS.gene027575:intron 30.0%
GATTAAGGCTGTATTTAGAT+TGG - chr5.1:25822885-25822904 MS.gene027575:CDS 30.0%
GTGTTTGAAAATATGAGCAA+TGG + chr5.1:25823336-25823355 None:intergenic 30.0%
TCTGATATTTAATTCCCTTC+AGG - chr5.1:25823427-25823446 MS.gene027575:CDS 30.0%
TGCTGAAATTTGCATTTCTT+AGG - chr5.1:25822738-25822757 MS.gene027575:intron 30.0%
TGTGTTCATAAACTATCCTT+GGG + chr5.1:25823032-25823051 None:intergenic 30.0%
TTGTGTTCATAAACTATCCT+TGG + chr5.1:25823033-25823052 None:intergenic 30.0%
TTTCACATCTAGATACACTT+AGG + chr5.1:25823154-25823173 None:intergenic 30.0%
TTTCTTCAGTTCTATTACCA+TGG - chr5.1:25822205-25822224 MS.gene027575:intron 30.0%
! ATGTTGAACCAATGATTTTG+AGG + chr5.1:25822260-25822279 None:intergenic 30.0%
! CTACAAACTGAGACATTTTT+GGG - chr5.1:25822934-25822953 MS.gene027575:CDS 30.0%
! CTTGGTCAAATTAGTTCTTA+TGG + chr5.1:25822788-25822807 None:intergenic 30.0%
! TGCTCATATTTTCAAACACA+TGG - chr5.1:25823337-25823356 MS.gene027575:CDS 30.0%
! TGTTGAACCAATGATTTTGA+GGG + chr5.1:25822259-25822278 None:intergenic 30.0%
! TTTTATAGAGGACTTACTGA+TGG - chr5.1:25822554-25822573 MS.gene027575:intron 30.0%
!! TCATCAGTAGCAATTTTAGT+TGG - chr5.1:25823232-25823251 MS.gene027575:CDS 30.0%
!!! CATCAGTAGCAATTTTAGTT+GGG - chr5.1:25823233-25823252 MS.gene027575:CDS 30.0%
AAAGCTAGAACTACTAGATG+CGG - chr5.1:25823171-25823190 MS.gene027575:CDS 35.0%
AACCAAAATTGAACCGGATT+TGG + chr5.1:25822366-25822385 None:intergenic 35.0%
AACTTCATAACGATGTTGAG+GGG + chr5.1:25821838-25821857 None:intergenic 35.0%
ACCAAGAATATGTATTCCCT+TGG + chr5.1:25822806-25822825 None:intergenic 35.0%
ACCAAGGGAATACATATTCT+TGG - chr5.1:25822802-25822821 MS.gene027575:CDS 35.0%
ATAAATGTAAGGCGAATCCT+TGG + chr5.1:25822663-25822682 None:intergenic 35.0%
ATATGAGCAATGGGAATTTC+GGG + chr5.1:25823326-25823345 None:intergenic 35.0%
CAACTTCATAACGATGTTGA+GGG + chr5.1:25821839-25821858 None:intergenic 35.0%
CCAAAAATGTCTCAGTTTGT+AGG + chr5.1:25822936-25822955 None:intergenic 35.0%
GATATTTAATTCCCTTCAGG+AGG - chr5.1:25823430-25823449 MS.gene027575:CDS 35.0%
TAAATGTAAGGCGAATCCTT+GGG + chr5.1:25822662-25822681 None:intergenic 35.0%
TACTCGAACCAAAATTGAAC+CGG + chr5.1:25822372-25822391 None:intergenic 35.0%
TACTTCCAAAACACCAAATC+CGG - chr5.1:25822350-25822369 MS.gene027575:intron 35.0%
TATTTAATTCCCTTCAGGAG+GGG - chr5.1:25823432-25823451 MS.gene027575:CDS 35.0%
TTCGCCTTACATTTATAGCT+TGG - chr5.1:25822667-25822686 MS.gene027575:intron 35.0%
TTGGCTATATACATATCCAG+GGG - chr5.1:25822835-25822854 MS.gene027575:CDS 35.0%
! CCTACAAACTGAGACATTTT+TGG - chr5.1:25822933-25822952 MS.gene027575:CDS 35.0%
! CCTTCTAAGAGATTTATCCA+CGG + chr5.1:25822693-25822712 None:intergenic 35.0%
! GATTGGCTATATACATATCC+AGG - chr5.1:25822833-25822852 MS.gene027575:CDS 35.0%
! TCGGGACGAGAATATATTTT+CGG + chr5.1:25823308-25823327 None:intergenic 35.0%
!! ACACTGATTGGGTTTTATAG+AGG - chr5.1:25822542-25822561 MS.gene027575:intron 35.0%
!!! ATTTGGTGTTTTGGAAGTAG+AGG + chr5.1:25822349-25822368 None:intergenic 35.0%
AACTATCCTTGGGAGTTTGT+TGG + chr5.1:25823022-25823041 None:intergenic 40.0%
AAGCTAGAACTACTAGATGC+GGG - chr5.1:25823172-25823191 MS.gene027575:CDS 40.0%
AATTACATACCCCAGGATCA+AGG - chr5.1:25822119-25822138 MS.gene027575:intron 40.0%
ACGGCCAAGCTATAAATGTA+AGG + chr5.1:25822674-25822693 None:intergenic 40.0%
ACTACTAGATGCGGGTAAAA+TGG - chr5.1:25823180-25823199 MS.gene027575:CDS 40.0%
ATTACATACCCCAGGATCAA+GGG - chr5.1:25822120-25822139 MS.gene027575:intron 40.0%
ATTCATGACCCATGAGAACA+AGG + chr5.1:25822102-25822121 None:intergenic 40.0%
ATTCTTTCACTCTCCTCCTT+AGG - chr5.1:25822233-25822252 MS.gene027575:intron 40.0%
CAACATCGTTATGAAGTTGG+TGG - chr5.1:25821840-25821859 MS.gene027575:CDS 40.0%
CCAACTTCATAACGATGTTG+AGG + chr5.1:25821840-25821859 None:intergenic 40.0%
CCGTGGATAAATCTCTTAGA+AGG - chr5.1:25822690-25822709 MS.gene027575:intron 40.0%
CCTCAACATCGTTATGAAGT+TGG - chr5.1:25821837-25821856 MS.gene027575:CDS 40.0%
CTGCGAGTAAACAGACTATT+GGG + chr5.1:25822415-25822434 None:intergenic 40.0%
CTTATTCCAACAAACTCCCA+AGG - chr5.1:25823013-25823032 MS.gene027575:CDS 40.0%
GTTGGAAAAACAGCTTATGG+TGG - chr5.1:25822163-25822182 MS.gene027575:intron 40.0%
TACTCATAGTCATCGGAACA+TGG + chr5.1:25823693-25823712 None:intergenic 40.0%
TCTGCGAGTAAACAGACTAT+TGG + chr5.1:25822416-25822435 None:intergenic 40.0%
TTACATTTATAGCTTGGCCG+TGG - chr5.1:25822673-25822692 MS.gene027575:intron 40.0%
! AAAAACAGCTTATGGTGGAG+TGG - chr5.1:25822168-25822187 MS.gene027575:intron 40.0%
! CAAGTGAGGAACTGATTTCT+CGG - chr5.1:25823478-25823497 MS.gene027575:CDS 40.0%
! CACCAAATCCGGTTCAATTT+TGG - chr5.1:25822361-25822380 MS.gene027575:intron 40.0%
! CGAGTTGGAAAAACAGCTTA+TGG - chr5.1:25822160-25822179 MS.gene027575:intron 40.0%
! TGATGGTGAAGAAATGCAGT+TGG - chr5.1:25822571-25822590 MS.gene027575:intron 40.0%
! TTTGAAGGCGTAGTTATGAG+AGG + chr5.1:25823660-25823679 None:intergenic 40.0%
!!! CATGGCACATCTGTTTTTGA+AGG + chr5.1:25823675-25823694 None:intergenic 40.0%
!!! TTGAACCGGATTTGGTGTTT+TGG + chr5.1:25822358-25822377 None:intergenic 40.0%
CAAAAAGACAGCGACAATGG+TGG + chr5.1:25822048-25822067 None:intergenic 45.0%
CAAGTGCTTGCCGTAATTAC+CGG - chr5.1:25821906-25821925 MS.gene027575:CDS 45.0%
CATTGGACGGCATCTTCTAA+AGG - chr5.1:25823455-25823474 MS.gene027575:CDS 45.0%
CCTTAGGCCCTCAAAATCAT+TGG - chr5.1:25822249-25822268 MS.gene027575:intron 45.0%
GAGTGAAAGAATTGCTGCCA+TGG + chr5.1:25822225-25822244 None:intergenic 45.0%
GCAAGCACTTGAGAAGTTGA+TGG + chr5.1:25821897-25821916 None:intergenic 45.0%
GGCTGAAGAGGATGAAAAGT+GGG + chr5.1:25822010-25822029 None:intergenic 45.0%
GGTCATGAATTACATACCCC+AGG - chr5.1:25822112-25822131 MS.gene027575:intron 45.0%
TACAGGAAGGCCGGTAATTA+CGG + chr5.1:25821919-25821938 None:intergenic 45.0%
TCCAGTGAGTGTTCCAAGAA+GGG + chr5.1:25821973-25821992 None:intergenic 45.0%
TTCGTCTCTGCAAAGTCAAC+AGG + chr5.1:25823614-25823633 None:intergenic 45.0%
! CACCATTGTCGCTGTCTTTT+TGG - chr5.1:25822046-25822065 MS.gene027575:intron 45.0%
! GCATCTTCTAAAGGCAAGTG+AGG - chr5.1:25823464-25823483 MS.gene027575:CDS 45.0%
! TGGTTAGTTCGGAGTTTGTG+AGG + chr5.1:25822304-25822323 None:intergenic 45.0%
!! ATGATTTTGAGGGCCTAAGG+AGG + chr5.1:25822249-25822268 None:intergenic 45.0%
!! CCAATGATTTTGAGGGCCTA+AGG + chr5.1:25822252-25822271 None:intergenic 45.0%
!! GTGTTGCAGGTTGGTTAGTT+CGG + chr5.1:25822315-25822334 None:intergenic 45.0%
!!! GGTGTTTTGGAAGTAGAGGA+AGG + chr5.1:25822345-25822364 None:intergenic 45.0%
ACACTGGTATCGTAACAGCG+TGG + chr5.1:25821813-25821832 None:intergenic 50.0%
ACGATGTTGAGGGGTAACAC+TGG + chr5.1:25821829-25821848 None:intergenic 50.0%
ATGGTGGCTGGAACAAGAAC+AGG + chr5.1:25822032-25822051 None:intergenic 50.0%
ATGTAAGGCGAATCCTTGGG+AGG + chr5.1:25822659-25822678 None:intergenic 50.0%
CGCCAAAAAGACAGCGACAA+TGG + chr5.1:25822051-25822070 None:intergenic 50.0%
CTTGGAACACTCACTGGACT+GGG - chr5.1:25821975-25821994 MS.gene027575:CDS 50.0%
GAAACTACACATTCAGCCCC+TGG + chr5.1:25822854-25822873 None:intergenic 50.0%
GAACAAGAACAGGGCTGAAG+AGG + chr5.1:25822022-25822041 None:intergenic 50.0%
GGAAGTAGAGGAAGGATTCG+AGG + chr5.1:25822337-25822356 None:intergenic 50.0%
GGCACTGTCCTTGTTCTCAT+GGG - chr5.1:25822091-25822110 MS.gene027575:intron 50.0%
GGGCTGAAGAGGATGAAAAG+TGG + chr5.1:25822011-25822030 None:intergenic 50.0%
GGTGAAGAAATGCAGTTGGC+TGG - chr5.1:25822575-25822594 MS.gene027575:intron 50.0%
GTAATTACCGGCCTTCCTGT+AGG - chr5.1:25821918-25821937 MS.gene027575:CDS 50.0%
GTCCAGTGAGTGTTCCAAGA+AGG + chr5.1:25821974-25821993 None:intergenic 50.0%
TCCCTTCTTGGAACACTCAC+TGG - chr5.1:25821969-25821988 MS.gene027575:CDS 50.0%
TCTACAGGAGCAACTCGAGT+TGG - chr5.1:25822145-25822164 MS.gene027575:intron 50.0%
TCTTGGAACACTCACTGGAC+TGG - chr5.1:25821974-25821993 MS.gene027575:CDS 50.0%
TGGCACTGTCCTTGTTCTCA+TGG - chr5.1:25822090-25822109 MS.gene027575:intron 50.0%
TGGTGGCTGGAACAAGAACA+GGG + chr5.1:25822031-25822050 None:intergenic 50.0%
TTGGAACACTCACTGGACTG+GGG - chr5.1:25821976-25821995 MS.gene027575:CDS 50.0%
! AACTCGAGTTGCTCCTGTAG+AGG + chr5.1:25822146-25822165 None:intergenic 50.0%
!! TGTCGCTGTCTTTTTGGCGT+CGG - chr5.1:25822052-25822071 MS.gene027575:intron 50.0%
!!! TTTTGGCGTCGGCAGCTTTT+GGG - chr5.1:25822063-25822082 MS.gene027575:intron 50.0%
!!! TTTTTGGCGTCGGCAGCTTT+TGG - chr5.1:25822062-25822081 MS.gene027575:intron 50.0%
AAGACAGCGACAATGGTGGC+TGG + chr5.1:25822044-25822063 None:intergenic 55.0%
AAGGACGTTGATGCCGCATG+TGG - chr5.1:25822517-25822536 MS.gene027575:intron 55.0%
ATTACCGGCCTTCCTGTAGG+CGG - chr5.1:25821921-25821940 MS.gene027575:CDS 55.0%
CCAATCAGTGTCGCCACATG+CGG + chr5.1:25822533-25822552 None:intergenic 55.0%
CTGGTATCGTAACAGCGTGG+TGG + chr5.1:25821810-25821829 None:intergenic 55.0%
CTGTAGAGGCCCTTGATCCT+GGG + chr5.1:25822132-25822151 None:intergenic 55.0%
CTTGCTGGACTCTCCCTTCT+TGG - chr5.1:25821957-25821976 MS.gene027575:CDS 55.0%
GGTGTACTGCTTGCACTTGC+TGG - chr5.1:25821942-25821961 MS.gene027575:CDS 55.0%
GTTTGTGAGGACTCGACGAG+TGG + chr5.1:25822291-25822310 None:intergenic 55.0%
TGTAGAGGCCCTTGATCCTG+GGG + chr5.1:25822131-25822150 None:intergenic 55.0%
! ATTCCCTTCAGGAGGGGCAT+TGG - chr5.1:25823438-25823457 MS.gene027575:CDS 55.0%
! CGCATGTGGCGACACTGATT+GGG - chr5.1:25822531-25822550 MS.gene027575:intron 55.0%
! GAGGACTCGACGAGTGGTTT+TGG + chr5.1:25822285-25822304 None:intergenic 55.0%
! TGGAACACTCACTGGACTGG+GGG - chr5.1:25821977-25821996 MS.gene027575:CDS 55.0%
!! GGAAGGATTCGAGGTGTTGC+AGG + chr5.1:25822328-25822347 None:intergenic 55.0%
!! GGATTCGAGGTGTTGCAGGT+TGG + chr5.1:25822324-25822343 None:intergenic 55.0%
AGGCGAATCCTTGGGAGGAG+TGG + chr5.1:25822654-25822673 None:intergenic 60.0%
CCAGGATCAAGGGCCTCTAC+AGG - chr5.1:25822130-25822149 MS.gene027575:intron 60.0%
CCGCATGTGGCGACACTGAT+TGG - chr5.1:25822530-25822549 MS.gene027575:intron 60.0%
CCTGTAGAGGCCCTTGATCC+TGG + chr5.1:25822133-25822152 None:intergenic 60.0%
CGTCCAATGCCCCTCCTGAA+GGG + chr5.1:25823444-25823463 None:intergenic 60.0%
GCAAGCAGTACACCGCCTAC+AGG + chr5.1:25821936-25821955 None:intergenic 60.0%
GCAGTACACCGCCTACAGGA+AGG + chr5.1:25821932-25821951 None:intergenic 60.0%
GGCGAATCCTTGGGAGGAGT+GGG + chr5.1:25822653-25822672 None:intergenic 60.0%
TACACCGCCTACAGGAAGGC+CGG + chr5.1:25821928-25821947 None:intergenic 60.0%
! GTCGGCAGCTTTTGGGCTGA+TGG - chr5.1:25822070-25822089 MS.gene027575:intron 60.0%
!! CCTTCAGGAGGGGCATTGGA+CGG - chr5.1:25823442-25823461 MS.gene027575:CDS 60.0%
CCGTCCAATGCCCCTCCTGA+AGG + chr5.1:25823445-25823464 None:intergenic 65.0%
TGCTTCGCCCACTCCTCCCA+AGG - chr5.1:25822643-25822662 MS.gene027575:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 25821792 25823713 25821792 ID=MS.gene027575
chr5.1 mRNA 25821792 25823713 25821792 ID=MS.gene027575.t1;Parent=MS.gene027575
chr5.1 exon 25822794 25823713 25822794 ID=MS.gene027575.t1.exon1;Parent=MS.gene027575.t1
chr5.1 CDS 25822794 25823713 25822794 ID=cds.MS.gene027575.t1;Parent=MS.gene027575.t1
chr5.1 exon 25821792 25822056 25821792 ID=MS.gene027575.t1.exon2;Parent=MS.gene027575.t1
chr5.1 CDS 25821792 25822056 25821792 ID=cds.MS.gene027575.t1;Parent=MS.gene027575.t1
Gene Sequence

>MS.gene027575

ATGTCAGAACTTACACCACCACGCTGTTACGATACCAGTGTTACCCCTCAACATCGTTATGAAGTTGGTGGAGTTACTACTCATTACTCAGAGAAAAGAAGTCCATCAACTTCTCAAGTGCTTGCCGTAATTACCGGCCTTCCTGTAGGCGGTGTACTGCTTGCACTTGCTGGACTCTCCCTTCTTGGAACACTCACTGGACTGGGGGTAACAAGCCCACTTTTCATCCTCTTCAGCCCTGTTCTTGTTCCAGCCACCATTGTCGCTGTCTTTTTGGCGTCGGCAGCTTTTGGGCTGATGGCACTGTCCTTGTTCTCATGGGTCATGAATTACATACCCCAGGATCAAGGGCCTCTACAGGAGCAACTCGAGTTGGAAAAACAGCTTATGGTGGAGTGGTGTAACATGAAAGTTTTCTTCAGTTCTATTACCATGGCAGCAATTCTTTCACTCTCCTCCTTAGGCCCTCAAAATCATTGGTTCAACATCACCAAAACCACTCGTCGAGTCCTCACAAACTCCGAACTAACCAACCTGCAACACCTCGAATCCTTCCTCTACTTCCAAAACACCAAATCCGGTTCAATTTTGGTTCGAGTAATGAGAGACAACGAAATTGACCCAATAGTCTGTTTACTCGCAGATGCGTTCGCTGAATTGATGTTGTTCCCGAAATGTTATATCAACATGTTGAGATTCCTTATGAAACAATATTTGATTAAACGAAGGACGTTGATGCCGCATGTGGCGACACTGATTGGGTTTTATAGAGGACTTACTGATGGTGAAGAAATGCAGTTGGCTGGAACTGTTGAGATTTGTTTTGATAAAAATGGTGCTAATTCTTCTCGTGCTTCGCCCACTCCTCCCAAGGATTCGCCTTACATTTATAGCTTGGCCGTGGATAAATCTCTTAGAAGGTAAACATTATTTATTGCGTCCAAGTTGCTGAAATTTGCATTTCTTAGGTAAAATTAGCTCAAGTGTCATGAACCATAAGAACTAATTTGACCAAGGGAATACATATTCTTGGTCTAATTTGATTGGCTATATACATATCCAGGGGCTGAATGTGTAGTTTCGATTATTCAAAGATTAAGGCTGTATTTAGATTGGGTTATGAATAACTTATTTGAGCTTACCTACAAACTGAGACATTTTTGGGTATGTTTGTTAGAGCTTATGAAAATAGTTCAAAACATGTCCATAAGCTGATTTTAGCTTATTCCAACAAACTCCCAAGGATAGTTTATGAACACAATTTGCAAATTATATGAAAACAGCTTAACTACATTTTCTCGTTGCTATTATAAGTAGCTTATACATAAGCGCTAAATTAAGTTGTTTATTCAAACATATCCTAAGTGTATCTAGATGTGAAAGCTAGAACTACTAGATGCGGGTAAAATGGATATTCAGAAACTAGTTTGATTACTATTTTCATCAGTAGCAATTTTAGTTGGGAAAACCTGCAATTTAAATAATTAAGCTACCAAGTTTAAGATTTAAGTAACCGAAAATATATTCTCGTCCCGAAATTCCCATTGCTCATATTTTCAAACACATGGTATGTCTTCTCATTAAGCAAAAGAAAAGAAAAAAACCGATTCTGTTTTAACTTTCTAAAAATCTGTTTCTGATATTTAATTCCCTTCAGGAGGGGCATTGGACGGCATCTTCTAAAGGCAAGTGAGGAACTGATTTCTCGGATGAGTTCATCAAGAGAAGTTTACTTGCATTGCAGAATGATTGATGAAGCTCCATTTAACATGTATACAAAAGCTGATTACAAAATTGTTACGTCAGATAGTATTTTAGTCCTGTTGACTTTGCAGAGACGAAAATACTTGATGTGCAAGAAACTTCCTCTCATAACTACGCCTTCAAAAACAGATGTGCCATGTTCCGATGACTATGAGTAG
Protein sequence

>MS.gene027575.t1

MSELTPPRCYDTSVTPQHRYEVGGVTTHYSEKRSPSTSQVLAVITGLPVGGVLLALAGLSLLGTLTGLGVTSPLFILFSPVLVPATIVAVFLASAAFGLMALSLFSWVMNYIPQDQGPLQEQLELEKQLMVEWCNMKVFFSSITMAAILSLSSLGPQNHWFNITKTTRRVLTNSELTNLQHLESFLYFQNTKSGSILVRVMRDNEIDPIVCLLADAFAELMLFPKCYINMLRFLMKQYLIKRRTLMPHVATLIGFYRGLTDGEEMQLAGTVEICFDKNGANSSRASPTPPKDSPYIYSLAVDKSLRRRGIGRHLLKASEELISRMSSSREVYLHCRMIDEAPFNMYTKADYKIVTSDSILVLLTLQRRKYLMCKKLPLITTPSKTDVPCSDDYE