AlfalfaGEDB | MS.gene027854

M. sativa cultivar XinJiangDaYe / MS.gene027854

KEGG: K08867
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027854.t1	XP_003617691.1	86.9	153	17	1	1	150	2	154	3.10E-66	261.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027854.t1	Q6ICW6	70.5	149	41	1	5	150	10	158	1.7e-52	206.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027854.t1	G7K4D5	86.9	153	17	1	1	150	2	154	2.3e-66	261.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027854.t1	MTR_5g094390	86.928	153	17	1	1	150	2	154	2.88e-94	272
MS.gene027854.t1	MTR_5g094390	86.928	153	17	1	1	150	2	154	6.71e-93	271
MS.gene027854.t1	MTR_7g029020	71.895	153	40	1	1	150	1	153	6.68e-74	223
MS.gene027854.t1	MTR_3g056010	70.588	153	42	1	1	150	1	153	3.39e-73	221
MS.gene027854.t1	MTR_3g056025	70.588	153	42	1	1	150	1	153	4.55e-73	221
MS.gene027854.t1	MTR_3g099940	68.182	154	45	2	1	150	1	154	4.79e-67	206
MS.gene027854.t1	MTR_0283s0050	60.784	153	54	2	1	150	1	150	1.09e-59	186
MS.gene027854.t1	MTR_5g097530	60.784	153	54	2	1	150	1	150	1.95e-59	186
MS.gene027854.t1	MTR_3g076650	50.980	153	72	1	1	150	1	153	1.89e-45	156
MS.gene027854.t1	MTR_7g114680	47.222	144	73	1	10	150	8	151	1.88e-39	141
MS.gene027854.t1	MTR_8g027265	49.286	140	68	1	14	150	26	165	2.79e-38	137
MS.gene027854.t1	MTR_1g081330	47.222	144	73	1	10	150	8	151	3.49e-38	137
MS.gene027854.t1	MTR_3g080960	46.207	145	75	1	9	150	3	147	1.16e-37	135
MS.gene027854.t1	MTR_1g103850	45.946	148	77	1	6	150	4	151	2.44e-37	135
MS.gene027854.t1	MTR_7g072860	48.201	139	69	1	15	150	17	155	2.00e-36	132
MS.gene027854.t1	MTR_7g072640	48.201	139	69	1	15	150	17	155	2.00e-36	132
MS.gene027854.t1	MTR_5g075220	45.455	143	75	1	11	150	12	154	1.09e-35	129
MS.gene027854.t1	MTR_3g095680	48.175	137	68	1	17	150	24	160	2.72e-34	126
MS.gene027854.t1	MTR_3g095680	48.175	137	68	1	17	150	56	192	2.86e-34	126
MS.gene027854.t1	MTR_4g028830	45.324	139	73	1	15	150	13	151	2.97e-34	126
MS.gene027854.t1	MTR_8g093090	42.177	147	82	1	7	150	14	160	2.39e-32	120
MS.gene027854.t1	MTR_8g093090	42.177	147	82	1	7	150	32	178	3.05e-32	120
MS.gene027854.t1	MTR_7g114680	46.825	126	64	1	28	150	12	137	6.95e-32	119
MS.gene027854.t1	MTR_6g016260	46.875	128	65	1	26	150	6	133	3.31e-29	112
MS.gene027854.t1	MTR_5g049520	52.874	87	38	1	61	144	77	163	1.66e-25	95.9
MS.gene027854.t1	MTR_8g093090	46.667	75	40	0	76	150	4	78	1.30e-17	79.0
MS.gene027854.t1	MTR_0068s0070	56.364	55	24	0	90	144	65	119	4.92e-17	72.8
MS.gene027854.t1	MTR_1g081330	42.424	66	38	0	85	150	1	66	4.62e-12	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027854.t1	AT5G55560	70.470	149	41	1	5	150	10	158	2.89e-70	214
MS.gene027854.t1	AT3G48260	54.610	141	59	3	14	150	10	149	1.40e-41	145
MS.gene027854.t1	AT3G04910	47.917	144	72	1	10	150	8	151	1.60e-39	141
MS.gene027854.t1	AT5G28080	46.528	144	74	1	10	150	9	152	5.94e-39	137
MS.gene027854.t1	AT3G18750	51.471	136	63	1	18	150	20	155	5.95e-38	135
MS.gene027854.t1	AT3G18750	51.471	136	63	1	18	150	20	155	5.95e-38	135
MS.gene027854.t1	AT5G41990	49.275	138	67	1	16	150	19	156	8.98e-38	135
MS.gene027854.t1	AT3G22420	46.154	143	74	1	11	150	9	151	1.62e-37	134
MS.gene027854.t1	AT3G18750	51.471	136	63	1	18	150	20	155	1.65e-37	134
MS.gene027854.t1	AT3G18750	51.471	136	63	1	18	150	20	155	1.65e-37	134
MS.gene027854.t1	AT3G18750	51.471	136	63	1	18	150	20	155	1.65e-37	134
MS.gene027854.t1	AT3G22420	46.154	143	74	1	11	150	9	151	1.73e-37	135
MS.gene027854.t1	AT3G22420	46.154	143	74	1	11	150	9	151	1.96e-37	135
MS.gene027854.t1	AT3G22420	46.154	143	74	1	11	150	9	151	2.00e-37	135
MS.gene027854.t1	AT3G22420	46.154	143	74	1	11	150	80	222	2.35e-37	135
MS.gene027854.t1	AT3G22420	46.154	143	74	1	11	150	80	222	2.65e-37	135
MS.gene027854.t1	AT1G49160	50.000	136	65	1	18	150	20	155	5.87e-37	133
MS.gene027854.t1	AT3G51630	49.315	146	71	2	8	150	7	152	7.60e-37	132
MS.gene027854.t1	AT3G51630	49.315	146	71	2	8	150	7	152	7.60e-37	132
MS.gene027854.t1	AT5G58350	44.928	138	73	1	16	150	9	146	1.71e-34	126
MS.gene027854.t1	AT3G18750	51.181	127	59	1	27	150	11	137	5.30e-34	125
MS.gene027854.t1	AT1G64630	46.377	138	71	1	16	150	6	143	1.17e-33	124
MS.gene027854.t1	AT1G49160	38.798	183	62	3	18	150	20	202	1.19e-29	113
MS.gene027854.t1	AT1G49160	50.442	113	53	1	41	150	25	137	1.21e-29	112
MS.gene027854.t1	AT1G49160	38.798	183	62	3	18	150	20	202	1.90e-29	112
MS.gene027854.t1	AT3G04910	46.154	117	60	1	37	150	12	128	2.49e-29	112
MS.gene027854.t1	AT5G28080	35.417	144	53	3	10	150	9	115	1.05e-20	87.4
MS.gene027854.t1	AT3G48260	62.264	53	20	0	98	150	2	54	8.24e-16	73.6
MS.gene027854.t1	AT5G28080	46.970	66	35	0	85	150	1	66	1.11e-14	70.5
MS.gene027854.t1	AT5G28080	46.970	66	35	0	85	150	1	66	1.11e-14	70.5
MS.gene027854.t1	AT5G28080	46.970	66	35	0	85	150	1	66	1.11e-14	70.5
MS.gene027854.t1	AT5G28080	46.970	66	35	0	85	150	1	66	1.11e-14	70.5
MS.gene027854.t1	AT5G28080	46.970	66	35	0	85	150	1	66	1.11e-14	70.5

Find 25 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCACCACATCCATATAGTT+TGG	0.326193	5.4:+21098069	None:intergenic
GGTAGATATCCCAAACTATA+TGG	0.346539	5.4:-21098079	MS.gene027854:CDS
CTATGAAGGCATTGAAGAAA+TGG	0.389560	5.4:-21097790	MS.gene027854:CDS
GGAGTCTTTGTCGGATGAAC+CGG	0.423531	5.4:+21098138	None:intergenic
TATCTACCATACCTACCAAT+TGG	0.455103	5.4:+21098094	None:intergenic
GCACCACATCCATATAGTTT+GGG	0.458771	5.4:+21098070	None:intergenic
CATGTAACCAAGTGAAGTTA+AGG	0.465729	5.4:-21097997	MS.gene027854:CDS
ACTGATCCAATTGGTAGGTA+TGG	0.472319	5.4:-21098100	MS.gene027854:CDS
GAGGGAATTTGAGAGAGTAT+AGG	0.484119	5.4:-21097832	MS.gene027854:CDS
TTTGCGAAAACTGATCCAAT+TGG	0.497824	5.4:-21098109	MS.gene027854:CDS
AGAAGTTCCTTAACTTCACT+TGG	0.505019	5.4:+21097990	None:intergenic
AAATGCCTCGGAGTCTTTGT+CGG	0.508219	5.4:+21098129	None:intergenic
ACATAAACATGTTTCTATGA+AGG	0.523139	5.4:-21097804	MS.gene027854:CDS
TAAAGCCTCTCTATCATAGC+AGG	0.530301	5.4:+21097959	None:intergenic
ATGACTGAGGTTTGTACAAG+AGG	0.553005	5.4:-21097851	MS.gene027854:CDS
TGACTGAGGTTTGTACAAGA+GGG	0.555985	5.4:-21097850	MS.gene027854:CDS
CGAAAACTGATCCAATTGGT+AGG	0.575212	5.4:-21098105	MS.gene027854:CDS
TATCCCAAACTATATGGATG+TGG	0.586654	5.4:-21098073	MS.gene027854:CDS
AGAGGCTTTATTCTGAAGTG+AGG	0.595040	5.4:-21097946	MS.gene027854:CDS
CGATCAAGAAGAAGTAATTG+AGG	0.603202	5.4:-21098023	MS.gene027854:CDS
ATGAACCTGCTATGATAGAG+AGG	0.634842	5.4:-21097964	MS.gene027854:CDS
AGGCATTGAAGAAATGGTCA+AGG	0.636906	5.4:-21097784	MS.gene027854:CDS
GATGAACCGGAGTTAACACT+CGG	0.701668	5.4:+21098151	None:intergenic
TAGATGCCGAGTGTTAACTC+CGG	0.702403	5.4:-21098157	MS.gene027854:CDS
TTCATCCGACAAAGACTCCG+AGG	0.705998	5.4:-21098134	MS.gene027854:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACATAAACATGTTTCTATGA+AGG	-	chr5.4:21098062-21098081	MS.gene027854:CDS	25.0%
!	CACACTTAATTTTATGACTG+AGG	-	chr5.4:21098002-21098021	MS.gene027854:CDS	30.0%
	AGAAGTTCCTTAACTTCACT+TGG	+	chr5.4:21097879-21097898	None:intergenic	35.0%
	CATGTAACCAAGTGAAGTTA+AGG	-	chr5.4:21097869-21097888	MS.gene027854:CDS	35.0%
	CGATCAAGAAGAAGTAATTG+AGG	-	chr5.4:21097843-21097862	MS.gene027854:CDS	35.0%
	GGTAGATATCCCAAACTATA+TGG	-	chr5.4:21097787-21097806	MS.gene027854:CDS	35.0%
	TATCCCAAACTATATGGATG+TGG	-	chr5.4:21097793-21097812	MS.gene027854:CDS	35.0%
	TATCTACCATACCTACCAAT+TGG	+	chr5.4:21097775-21097794	None:intergenic	35.0%
	TTTGCGAAAACTGATCCAAT+TGG	-	chr5.4:21097757-21097776	MS.gene027854:CDS	35.0%
!!	CTATGAAGGCATTGAAGAAA+TGG	-	chr5.4:21098076-21098095	MS.gene027854:CDS	35.0%
	ACTGATCCAATTGGTAGGTA+TGG	-	chr5.4:21097766-21097785	MS.gene027854:CDS	40.0%
	AGCACCACATCCATATAGTT+TGG	+	chr5.4:21097800-21097819	None:intergenic	40.0%
	ATGAACCTGCTATGATAGAG+AGG	-	chr5.4:21097902-21097921	MS.gene027854:CDS	40.0%
	ATGACTGAGGTTTGTACAAG+AGG	-	chr5.4:21098015-21098034	MS.gene027854:CDS	40.0%
	CGAAAACTGATCCAATTGGT+AGG	-	chr5.4:21097761-21097780	MS.gene027854:CDS	40.0%
	GCACCACATCCATATAGTTT+GGG	+	chr5.4:21097799-21097818	None:intergenic	40.0%
	TAAAGCCTCTCTATCATAGC+AGG	+	chr5.4:21097910-21097929	None:intergenic	40.0%
	TGACTGAGGTTTGTACAAGA+GGG	-	chr5.4:21098016-21098035	MS.gene027854:CDS	40.0%
!	ATCAGTTTTCGCAAATGCCT+CGG	+	chr5.4:21097752-21097771	None:intergenic	40.0%
!	ATGGTCAAGGCAGATTTTGA+AGG	-	chr5.4:21098095-21098114	MS.gene027854:CDS	40.0%
!	GAGGGAATTTGAGAGAGTAT+AGG	-	chr5.4:21098034-21098053	MS.gene027854:CDS	40.0%
!	TGGTCAAGGCAGATTTTGAA+GGG	-	chr5.4:21098096-21098115	MS.gene027854:CDS	40.0%
!!	AGAGGCTTTATTCTGAAGTG+AGG	-	chr5.4:21097920-21097939	MS.gene027854:CDS	40.0%
!!	AGGCATTGAAGAAATGGTCA+AGG	-	chr5.4:21098082-21098101	MS.gene027854:CDS	40.0%
	AAATGCCTCGGAGTCTTTGT+CGG	+	chr5.4:21097740-21097759	None:intergenic	45.0%
	GATGAACCGGAGTTAACACT+CGG	+	chr5.4:21097718-21097737	None:intergenic	45.0%
!!	GGTCAAGGCAGATTTTGAAG+GGG	-	chr5.4:21098097-21098116	MS.gene027854:CDS	45.0%
	GGAGTCTTTGTCGGATGAAC+CGG	+	chr5.4:21097731-21097750	None:intergenic	50.0%
	TTCATCCGACAAAGACTCCG+AGG	-	chr5.4:21097732-21097751	MS.gene027854:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	21097712	21098176	21097712	ID=MS.gene027854
chr5.4	mRNA	21097712	21098176	21097712	ID=MS.gene027854.t1;Parent=MS.gene027854
chr5.4	exon	21097712	21098176	21097712	ID=MS.gene027854.t1.exon1;Parent=MS.gene027854.t1
chr5.4	CDS	21097712	21098176	21097712	ID=cds.MS.gene027854.t1;Parent=MS.gene027854.t1

Gene Sequence

>MS.gene027854

ATGCCGAGTGTTAACTCCGGTTCATCCGACAAAGACTCCGAGGCATTTGCGAAAACTGATCCAATTGGTAGGTATGGTAGATATCCCAAACTATATGGATGTGGTGCTGTGAAGAAAGTGTACTGTGCATTCGATCAAGAAGAAGTAATTGAGGTAGCATGTAACCAAGTGAAGTTAAGGAACTTCTGTGATGAACCTGCTATGATAGAGAGGCTTTATTCTGAAGTGAGGTTATTGAGAAGCAAAAACATCATTGAACTTTATAGTGTTTCGAGCAATGATCGAAACAACACACTTAATTTTATGACTGAGGTTTGTACAAGAGGGAATTTGAGAGAGTATAGGAAGAAACATAAACATGTTTCTATGAAGGCATTGAAGAAATGGTCAAGGCAGATTTTGAAGGGGTTGAATGATTCGCACACTCATGAGTCTTGCATCATTCAGAGATCTGAACTGCAGTAA

Protein sequence

>MS.gene027854.t1

MPSVNSGSSDKDSEAFAKTDPIGRYGRYPKLYGCGAVKKVYCAFDQEEVIEVACNQVKLRNFCDEPAMIERLYSEVRLLRSKNIIELYSVSSNDRNNTLNFMTEVCTRGNLREYRKKHKHVSMKALKKWSRQILKGLNDSHTHESCIIQRSELQ