AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027880.t1 MTR_5g030430 94.737 684 23 4 1 678 1 677 0.0 1323
MS.gene027880.t1 MTR_8g067280 60.432 695 206 18 2 678 3 646 0.0 748
MS.gene027880.t1 MTR_5g030420 83.478 230 36 2 21 249 4 232 9.68e-136 398
MS.gene027880.t1 MTR_5g030420 80.932 236 43 2 21 255 4 238 1.13e-135 399
MS.gene027880.t1 MTR_8g024790 50.000 202 90 4 475 675 272 463 1.20e-53 191
MS.gene027880.t1 MTR_8g024790 32.796 186 92 2 45 226 25 181 4.28e-30 124
MS.gene027880.t1 MTR_8g024770 51.980 202 88 4 475 674 260 454 2.73e-53 190
MS.gene027880.t1 MTR_8g024770 30.319 188 83 3 59 237 36 184 5.25e-25 108
MS.gene027880.t1 MTR_7g096350 43.541 209 79 3 467 671 328 501 2.28e-41 158
MS.gene027880.t1 MTR_7g096350 28.723 188 118 5 45 226 49 226 3.87e-14 75.9
MS.gene027880.t1 MTR_8g027495 42.437 238 107 6 442 671 402 617 2.04e-36 145
MS.gene027880.t1 MTR_7g117670 41.414 198 92 4 484 678 392 568 1.50e-34 139
MS.gene027880.t1 MTR_7g117670 27.642 246 152 5 3 228 8 247 3.99e-21 98.2
MS.gene027880.t1 MTR_1g072320 33.684 190 105 6 46 226 11 188 1.58e-24 109
MS.gene027880.t1 MTR_1g072320 31.217 189 116 4 472 656 451 629 1.49e-18 90.5
MS.gene027880.t1 MTR_8g098275 34.104 173 94 6 61 223 19 181 6.03e-22 100
MS.gene027880.t1 MTR_8g098275 46.377 69 35 1 490 556 397 465 1.44e-11 68.2
MS.gene027880.t1 MTR_5g014640 34.810 158 85 3 483 640 147 286 4.20e-18 86.3
MS.gene027880.t1 MTR_2g104490 25.911 247 156 5 396 641 51 271 1.06e-16 82.0
MS.gene027880.t1 MTR_2g104490 25.911 247 156 5 396 641 51 271 1.25e-16 81.6
MS.gene027880.t1 MTR_4g067010 31.707 164 89 5 481 641 137 280 1.68e-16 81.6
MS.gene027880.t1 MTR_3g498695 31.000 200 106 6 492 675 296 479 5.62e-16 81.6
MS.gene027880.t1 MTR_4g057270 30.488 164 100 4 484 646 142 292 6.05e-16 80.1
MS.gene027880.t1 MTR_2g010450 26.872 227 129 7 422 640 116 313 7.93e-16 80.1
MS.gene027880.t1 MTR_0250s0040 31.056 161 100 2 494 654 88 237 1.16e-15 78.2
MS.gene027880.t1 MTR_0246s0020 30.481 187 106 4 480 660 190 358 1.99e-15 79.0
MS.gene027880.t1 MTR_4g066460 27.753 227 127 5 415 641 99 288 2.55e-15 78.2
MS.gene027880.t1 MTR_0194s0050 26.906 223 122 8 471 664 159 369 3.27e-15 78.6
MS.gene027880.t1 MTR_3g498695 29.851 201 108 6 492 675 296 480 3.60e-15 79.0
MS.gene027880.t1 MTR_4g092700 31.579 152 87 2 492 640 156 293 3.81e-15 77.8
MS.gene027880.t1 MTR_4g066380 28.155 206 113 4 436 641 115 285 4.13e-15 77.4
MS.gene027880.t1 MTR_3g030700 32.022 178 95 4 482 649 184 345 4.86e-15 77.8
MS.gene027880.t1 MTR_0246s0020 31.548 168 91 4 480 641 190 339 2.98e-14 75.5
MS.gene027880.t1 MTR_5g014520 30.723 166 100 4 477 641 142 293 3.97e-14 74.7
MS.gene027880.t1 MTR_2g084280 30.675 163 92 4 498 656 137 282 4.56e-14 73.9
MS.gene027880.t1 MTR_0011s0260 29.091 165 97 3 494 651 183 334 4.91e-14 74.7
MS.gene027880.t1 MTR_4g009110 29.878 164 99 3 482 641 162 313 5.51e-14 74.3
MS.gene027880.t1 MTR_4g097950 33.600 125 77 2 494 617 70 189 5.82e-14 72.8
MS.gene027880.t1 MTR_2g038040 32.026 153 96 3 495 642 53 202 6.74e-14 72.4
MS.gene027880.t1 MTR_3g104100 30.526 190 99 6 498 670 199 372 7.43e-14 74.3
MS.gene027880.t1 MTR_2g081040 31.122 196 100 6 498 675 181 359 7.50e-14 73.9
MS.gene027880.t1 MTR_2g104550 27.684 177 108 3 470 641 114 275 1.09e-13 73.2
MS.gene027880.t1 MTR_0011s0210 27.778 162 78 3 494 641 183 319 1.11e-13 73.6
MS.gene027880.t1 MTR_6g047570 32.903 155 79 4 494 641 159 295 1.75e-13 72.8
MS.gene027880.t1 MTR_4g009540 30.286 175 87 5 480 641 142 294 1.94e-13 72.0
MS.gene027880.t1 MTR_4g009540 30.286 175 87 5 480 641 142 294 2.22e-13 72.4
MS.gene027880.t1 MTR_1g112680 28.241 216 132 6 473 670 301 511 2.41e-13 73.6
MS.gene027880.t1 MTR_4g097940 30.539 167 94 5 475 641 140 284 3.94e-13 71.6
MS.gene027880.t1 MTR_5g014600 26.009 223 134 6 464 671 115 321 5.47e-13 71.2
MS.gene027880.t1 MTR_8g066770 28.409 176 113 3 493 660 114 284 6.17e-13 70.9
MS.gene027880.t1 MTR_5g064950 27.273 209 135 7 478 670 78 285 1.19e-12 69.7
MS.gene027880.t1 MTR_8g064700 29.282 181 107 6 476 650 234 399 1.25e-12 70.9
MS.gene027880.t1 MTR_7g110810 29.016 193 133 2 353 544 73 262 1.53e-12 70.1
MS.gene027880.t1 MTR_5g014560 30.719 153 95 2 494 646 131 272 2.25e-12 68.9
MS.gene027880.t1 MTR_7g083900 55.769 52 23 0 492 543 284 335 3.89e-12 69.3
MS.gene027880.t1 MTR_7g083900 55.769 52 23 0 492 543 284 335 4.02e-12 69.3
MS.gene027880.t1 MTR_6g047550 55.556 54 24 0 494 547 173 226 7.06e-12 67.0
MS.gene027880.t1 MTR_8g098275 47.761 67 33 1 490 554 240 306 8.93e-12 68.2
MS.gene027880.t1 MTR_8g098275 46.377 69 35 1 490 556 304 372 1.04e-11 68.2
MS.gene027880.t1 MTR_8g009020 27.011 174 105 4 478 641 125 286 1.05e-11 67.0
MS.gene027880.t1 MTR_4g097920 31.788 151 79 6 494 641 140 269 1.61e-11 66.2
MS.gene027880.t1 MTR_2g104560 29.936 157 77 5 495 641 55 188 1.80e-11 65.1
MS.gene027880.t1 MTR_4g097920 30.952 168 89 7 480 641 138 284 1.93e-11 66.2
MS.gene027880.t1 MTR_0246s0050 28.859 149 76 4 494 636 151 275 5.65e-11 64.7
MS.gene027880.t1 MTR_4g098035 28.859 149 76 4 494 636 172 296 7.02e-11 64.7
MS.gene027880.t1 MTR_4g094762 28.796 191 116 5 495 677 293 471 7.68e-11 65.5
MS.gene027880.t1 MTR_5g030770 28.814 177 99 4 482 653 141 295 8.52e-11 64.3
MS.gene027880.t1 MTR_1g028470 25.714 210 121 5 477 657 71 274 8.66e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027880.t1 AT1G32640 52.266 706 214 21 1 675 10 623 0.0 615
MS.gene027880.t1 AT5G46760 49.271 686 230 17 3 676 11 590 0.0 529
MS.gene027880.t1 AT4G17880 64.925 268 63 6 411 677 351 588 1.43e-105 333
MS.gene027880.t1 AT4G17880 56.985 272 102 5 2 264 22 287 1.29e-92 299
MS.gene027880.t1 AT5G46830 54.974 191 71 3 485 674 334 510 1.44e-54 195
MS.gene027880.t1 AT5G46830 46.025 239 107 7 5 232 4 231 8.52e-53 190
MS.gene027880.t1 AT1G01260 40.741 243 99 5 434 671 373 575 1.07e-41 160
MS.gene027880.t1 AT1G01260 31.034 232 150 3 3 226 7 236 1.24e-28 121
MS.gene027880.t1 AT1G01260 40.741 243 99 5 434 671 373 575 1.07e-41 160
MS.gene027880.t1 AT1G01260 31.034 232 150 3 3 226 7 236 1.24e-28 121
MS.gene027880.t1 AT1G01260 40.741 243 99 5 434 671 373 575 1.07e-41 160
MS.gene027880.t1 AT1G01260 31.034 232 150 3 3 226 7 236 1.24e-28 121
MS.gene027880.t1 AT2G46510 42.534 221 89 4 460 678 368 552 3.45e-41 159
MS.gene027880.t1 AT2G46510 33.333 234 143 4 4 226 8 239 4.16e-27 116
MS.gene027880.t1 AT4G16430 41.799 189 76 2 467 655 293 447 1.43e-37 146
MS.gene027880.t1 AT4G16430 29.348 184 120 4 46 226 50 226 7.79e-14 74.7
MS.gene027880.t1 AT4G00870 44.828 203 92 5 479 670 224 417 1.23e-35 140
MS.gene027880.t1 AT4G00870 31.188 202 104 6 26 226 22 189 8.85e-21 95.9
MS.gene027880.t1 AT4G09820 33.333 204 115 6 34 226 8 201 2.92e-26 114
MS.gene027880.t1 AT4G09820 30.719 153 75 3 491 643 360 481 5.95e-15 78.6
MS.gene027880.t1 AT1G63650 33.831 201 106 6 44 226 11 202 3.83e-23 104
MS.gene027880.t1 AT1G63650 26.455 189 131 3 490 670 401 589 1.80e-14 77.4
MS.gene027880.t1 AT1G63650 33.831 201 106 6 44 226 11 202 3.83e-23 104
MS.gene027880.t1 AT1G63650 26.455 189 131 3 490 670 401 589 1.80e-14 77.4
MS.gene027880.t1 AT1G63650 33.831 201 106 6 44 226 11 202 3.83e-23 104
MS.gene027880.t1 AT1G63650 26.455 189 131 3 490 670 401 589 1.80e-14 77.4
MS.gene027880.t1 AT5G41315 30.097 206 124 6 39 226 3 206 2.12e-20 96.3
MS.gene027880.t1 AT5G41315 26.263 198 129 4 488 670 435 630 4.25e-13 73.2
MS.gene027880.t1 AT5G41315 30.097 206 124 6 39 226 11 214 2.43e-20 96.3
MS.gene027880.t1 AT5G41315 26.263 198 129 4 488 670 443 638 4.05e-13 73.2
MS.gene027880.t1 AT5G41315 30.097 206 124 6 39 226 8 211 2.63e-20 95.9
MS.gene027880.t1 AT5G41315 26.263 198 129 4 488 670 440 635 4.63e-13 72.8
MS.gene027880.t1 AT4G00480 32.240 183 100 4 61 223 36 214 1.80e-19 92.8
MS.gene027880.t1 AT4G00480 32.240 183 100 4 61 223 36 214 2.26e-19 92.4
MS.gene027880.t1 AT4G00480 32.240 183 100 4 61 223 36 214 2.93e-19 92.4
MS.gene027880.t1 AT4G37850 32.973 185 92 6 472 641 123 290 1.15e-17 85.1
MS.gene027880.t1 AT4G37850 32.973 185 92 6 472 641 123 290 1.15e-17 85.1
MS.gene027880.t1 AT5G57150 30.526 190 110 6 495 675 55 231 1.96e-16 80.1
MS.gene027880.t1 AT2G28160 32.877 146 88 4 498 642 135 271 4.37e-16 80.5
MS.gene027880.t1 AT2G28160 32.877 146 88 4 498 642 135 271 6.12e-16 79.7
MS.gene027880.t1 AT5G57150 29.651 172 101 5 495 657 56 216 9.55e-16 77.4
MS.gene027880.t1 AT5G57150 29.651 172 101 5 495 657 56 216 1.42e-15 77.0
MS.gene027880.t1 AT5G57150 29.825 171 100 5 495 656 55 214 1.88e-15 76.6
MS.gene027880.t1 AT5G57150 29.545 176 104 5 495 661 56 220 2.07e-15 77.4
MS.gene027880.t1 AT4G29930 33.566 143 77 3 495 636 55 180 2.28e-14 73.6
MS.gene027880.t1 AT3G24140 25.592 211 132 6 476 664 177 384 3.03e-14 75.9
MS.gene027880.t1 AT2G22750 30.488 164 104 4 480 641 140 295 3.40e-14 74.7
MS.gene027880.t1 AT2G22750 31.288 163 104 3 480 641 140 295 3.62e-14 74.7
MS.gene027880.t1 AT2G22750 31.707 164 102 4 480 641 112 267 3.98e-14 74.3
MS.gene027880.t1 AT2G22750 31.707 164 102 4 480 641 112 267 4.04e-14 74.3
MS.gene027880.t1 AT2G22750 31.288 163 104 3 480 641 136 291 4.14e-14 74.7
MS.gene027880.t1 AT3G24140 25.592 211 132 6 476 664 177 384 4.95e-14 75.1
MS.gene027880.t1 AT3G26744 30.899 178 108 6 492 663 305 473 6.13e-14 75.1
MS.gene027880.t1 AT1G12860 31.176 170 88 4 492 649 265 417 1.22e-13 73.9
MS.gene027880.t1 AT4G29930 31.210 157 88 4 495 636 55 206 1.41e-13 72.0
MS.gene027880.t1 AT4G29930 31.210 157 88 4 495 636 55 206 1.41e-13 72.0
MS.gene027880.t1 AT1G12860 29.798 198 108 5 492 675 265 445 1.49e-13 73.9
MS.gene027880.t1 AT3G26744 31.707 164 99 5 492 649 305 461 1.52e-13 73.9
MS.gene027880.t1 AT3G26744 31.707 164 99 5 492 649 305 461 1.52e-13 73.9
MS.gene027880.t1 AT3G26744 31.707 164 99 5 492 649 305 461 1.52e-13 73.9
MS.gene027880.t1 AT4G29930 31.210 157 88 4 495 636 55 206 2.40e-13 70.9
MS.gene027880.t1 AT4G29930 32.168 143 76 3 495 636 55 177 2.41e-13 70.5
MS.gene027880.t1 AT4G29930 32.168 143 76 3 495 636 55 177 4.62e-13 69.7
MS.gene027880.t1 AT4G29930 44.000 75 42 0 496 570 56 130 8.67e-13 67.8
MS.gene027880.t1 AT2G16910 33.136 169 76 6 498 642 318 473 8.84e-13 72.0
MS.gene027880.t1 AT2G22770 26.486 185 120 4 488 660 123 303 5.69e-12 67.8
MS.gene027880.t1 AT2G22760 26.638 229 121 8 421 641 69 258 8.10e-12 67.0
MS.gene027880.t1 AT2G22760 28.780 205 99 8 421 617 69 234 1.03e-11 67.0
MS.gene027880.t1 AT5G65640 30.583 206 107 9 480 675 166 345 1.80e-11 66.6

Find 161 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATATCGATGTTAAGATTAT+AGG 0.170893 5.4:+20599927 MS.gene027880:CDS
TTATTGCTTCCTCTCGAATT+TGG 0.222345 5.4:-20599375 None:intergenic
ACAACAAGCAACCGTGAAAA+TGG 0.224565 5.4:+20600079 MS.gene027880:CDS
TGATTCGAAGAGGGTTTATT+TGG 0.231042 5.4:-20599888 None:intergenic
ACTTCCATTTCATGAATCTT+TGG 0.243626 5.4:+20598122 None:intergenic
TGTTGTTGTTGCTGCTGTTT+TGG 0.244944 5.4:-20599858 None:intergenic
AGTTCTTATGTTGCTAATAA+TGG 0.260796 5.4:+20599252 MS.gene027880:CDS
CAACCTTCTTACGACTATTC+AGG 0.266356 5.4:+20598334 MS.gene027880:CDS
GAAATGAATCTTTCTGATTA+TGG 0.280620 5.4:+20599156 MS.gene027880:CDS
GATGGATAAAGCTTCACTTT+TGG 0.289543 5.4:+20599698 MS.gene027880:CDS
CACAAAATCTCACCGGATTC+CGG 0.303867 5.4:-20599210 None:intergenic
AGGTTCTTCGTTACTTGGTT+GGG 0.304524 5.4:+20598354 MS.gene027880:CDS
TATTCAGGTTCTTCGTTACT+TGG 0.315314 5.4:+20598349 MS.gene027880:CDS
ACCATCGGCTGACGGTGTTT+TGG 0.319919 5.4:+20598723 MS.gene027880:CDS
TCAGCTCCGGTGTGGTTAAC+TGG 0.321901 5.4:+20598622 MS.gene027880:CDS
TGTTCTGTTGTTGGTGCTGC+TGG 0.321937 5.4:-20598996 None:intergenic
TTGAACATCCCCGACTTTAG+AGG 0.323506 5.4:-20600144 None:intergenic
AGAATGATCCTTCTTCGATT+TGG 0.325148 5.4:+20598875 MS.gene027880:CDS
CCACCCGGCAGCAAGGTTAA+TGG 0.328981 5.4:+20599989 MS.gene027880:CDS
TTCTTCTGTAACCGGATTAC+CGG 0.334316 5.4:-20598494 None:intergenic
TTTGGATCTTCTTGGCAGTT+AGG 0.342187 5.4:+20598832 MS.gene027880:CDS
CACTGGCATGGTGCACTTCT+AGG 0.343448 5.4:-20600031 None:intergenic
TATTGTTATTGTTCTGTTGT+TGG 0.349299 5.4:-20599005 None:intergenic
CCTCTGATTTATCAACCTTA+TGG 0.351613 5.4:+20598185 MS.gene027880:CDS
GGTTCCTCTCTTCCGTTTGC+CGG 0.358134 5.4:-20599585 None:intergenic
CTCCGGTGTTCTTGTTCAGC+AGG 0.359735 5.4:-20598439 None:intergenic
ACAGAACAATAACAATAATC+AGG 0.363674 5.4:+20599011 MS.gene027880:CDS
TTATCCATCTTTGAAACATT+AGG 0.366548 5.4:-20599684 None:intergenic
GGTTGTGACGGTGGTGGTGC+TGG 0.367399 5.4:-20598211 None:intergenic
TGGGGTGATGGTTACTATAA+AGG 0.374935 5.4:+20598373 MS.gene027880:CDS
AAGGTTAATGGCAGCGTTAA+TGG 0.379124 5.4:+20600001 MS.gene027880:CDS
TCGTTACTTGGTTGGGGTGA+TGG 0.379647 5.4:+20598361 MS.gene027880:CDS
CAGGTTCTTCGTTACTTGGT+TGG 0.382764 5.4:+20598353 MS.gene027880:CDS
TTGAAATTCTCTGGTGGTAC+TGG 0.385403 5.4:+20599456 MS.gene027880:CDS
TGTTGGTGCTGCTGGTGACT+TGG 0.388845 5.4:-20598988 None:intergenic
TTCATTAATCTCCGGTAATC+CGG 0.396662 5.4:+20598483 MS.gene027880:CDS
TCGAATTCGAATTTCTTCTC+TGG 0.399599 5.4:+20599279 MS.gene027880:CDS
ACAGGAGATTCTTCTGTAAC+CGG 0.415426 5.4:-20598502 None:intergenic
TTAAAGCTTGAAGACGTTGT+TGG 0.415771 5.4:-20598270 None:intergenic
TGTTCCTAATGTTTCAAAGA+TGG 0.422462 5.4:+20599680 MS.gene027880:CDS
CAACAAGCAACCGTGAAAAT+GGG 0.427408 5.4:+20600080 MS.gene027880:CDS
GCGTCGAATTTGAAATTCTC+TGG 0.430033 5.4:+20599447 MS.gene027880:CDS
TCAACAGAATCTCTAGCTTC+AGG 0.431024 5.4:-20598907 None:intergenic
GTGGTTTGTGGTGGTTGTGA+CGG 0.435088 5.4:-20598223 None:intergenic
TTGAATCCGGTGGTGGTTTG+TGG 0.438183 5.4:-20598235 None:intergenic
GAAGAGGGTTTATTTGGTAC+TGG 0.440122 5.4:-20599882 None:intergenic
TCAGTAACTTCTTCATCAAC+AGG 0.444377 5.4:-20598520 None:intergenic
TTCTCTGGTGGTACTGGTGG+TGG 0.450615 5.4:+20599462 MS.gene027880:CDS
GGCTTCCTCCCTCTCTTCCT+CGG 0.451066 5.4:-20599564 None:intergenic
GAAAAGAAGCCGAGGAAGAG+AGG 0.453055 5.4:+20599555 MS.gene027880:CDS
GAGGAAGAGAGGGAGGAAGC+CGG 0.453227 5.4:+20599566 MS.gene027880:CDS
GTCAACGGAACTGGGCTGCC+TGG 0.453582 5.4:+20598583 MS.gene027880:CDS
TGGTACTGGTGGTGGTGATT+CGG 0.453669 5.4:+20599470 MS.gene027880:CDS
GAGAGTGTCTTGGTTGAATC+CGG 0.454531 5.4:-20598248 None:intergenic
TCCTGGTTCGAGTACTTTAA+CGG 0.455187 5.4:+20599074 MS.gene027880:CDS
TCGAAGGCGCAAAAGAAGTC+TGG 0.457045 5.4:+20598293 MS.gene027880:CDS
TGGTGGTGCTGGTGGCCATA+AGG 0.458834 5.4:-20598200 None:intergenic
ACAACAATGGGAACGGGAAT+GGG 0.462203 5.4:+20599334 MS.gene027880:CDS
CGTTTGTTGAAGCCGGAATC+CGG 0.465479 5.4:+20599198 MS.gene027880:CDS
TGGTGGTGATTCGGACCATT+CGG 0.468581 5.4:+20599479 MS.gene027880:CDS
TGATTCGGACCATTCGGATC+TGG 0.469684 5.4:+20599485 MS.gene027880:CDS
CGGCATTGTCCTCTAAAGTC+GGG 0.471070 5.4:+20600135 MS.gene027880:CDS
GTTTGTGGTGGTTGTGACGG+TGG 0.471605 5.4:-20598220 None:intergenic
AAAACTGAATCTAATAAAGA+TGG 0.474843 5.4:+20599765 MS.gene027880:CDS
AACAACAATGGGAACGGGAA+TGG 0.482475 5.4:+20599333 MS.gene027880:CDS
CCATTCGGATCTGGAGGCTT+CGG 0.482750 5.4:+20599494 MS.gene027880:CDS
TAGGCTGGGATGCAATGATA+AGG 0.484555 5.4:+20599946 MS.gene027880:CDS
CGCCCTCTCACAAGCTGAGA+GGG 0.484643 5.4:-20598659 None:intergenic
GGTTCTTCGTTACTTGGTTG+GGG 0.488569 5.4:+20598355 MS.gene027880:CDS
TCTGGAGGCTTCGGTGGTGA+AGG 0.499262 5.4:+20599503 MS.gene027880:CDS
AATGGGAACGGGAATGGGAA+TGG 0.501146 5.4:+20599339 MS.gene027880:CDS
AGAGGAAGCAATAATGATGA+TGG 0.505617 5.4:+20599384 MS.gene027880:CDS
AATTTCAAATTCGACGCCCC+TGG 0.511045 5.4:-20599441 None:intergenic
CGATGTTAAGATTATAGGCT+GGG 0.511425 5.4:+20599932 MS.gene027880:CDS
GGCTTCGGTGGTGAAGGAGG+TGG 0.511799 5.4:+20599509 MS.gene027880:CDS
AAAAGAAGCCGAGGAAGAGA+GGG 0.514449 5.4:+20599556 MS.gene027880:CDS
CGCCCTCTCAGCTTGTGAGA+GGG 0.515362 5.4:+20598657 MS.gene027880:CDS
CTGCCATTAACCTTGCTGCC+GGG 0.517345 5.4:-20599992 None:intergenic
AGCTTATTATAATTCAGCTC+CGG 0.519595 5.4:+20598609 MS.gene027880:CDS
AGAGGGAGGAAGCCGGCAAA+CGG 0.519792 5.4:+20599573 MS.gene027880:CDS
CTTGAAACATGAACAACACT+AGG 0.520830 5.4:-20599102 None:intergenic
GAACTTAATTCATTAATCTC+CGG 0.523787 5.4:+20598475 MS.gene027880:CDS
ACGGGAATGGGAATGGAAAG+AGG 0.524874 5.4:+20599346 MS.gene027880:CDS
AGGCTGGGATGCAATGATAA+GGG 0.527237 5.4:+20599947 MS.gene027880:CDS
AGGAGAACAACAATGGGAAC+GGG 0.528523 5.4:+20599328 MS.gene027880:CDS
GTCTTGGTTGAATCCGGTGG+TGG 0.529478 5.4:-20598242 None:intergenic
GCTGCCATTAACCTTGCTGC+CGG 0.530316 5.4:-20599993 None:intergenic
AGGATCATTCTCACCATGAT+GGG 0.531136 5.4:-20598863 None:intergenic
TCTGGTGTAATTGTTCCACC+AGG 0.531186 5.4:+20599423 MS.gene027880:CDS
TCAGCAACGTTTGTTGAAGC+CGG 0.534264 5.4:+20599191 MS.gene027880:CDS
AAGGATCATTCTCACCATGA+TGG 0.538289 5.4:-20598864 None:intergenic
CGACGATAACTCTTCAGTAA+TGG 0.538731 5.4:+20598147 MS.gene027880:CDS
TTGCTGAGGAGAACAACAAT+GGG 0.545102 5.4:+20599322 MS.gene027880:CDS
GACGTTGTTGGAGAGTGTCT+TGG 0.546035 5.4:-20598258 None:intergenic
TGGTTCGAGTACTTTAACGG+AGG 0.547059 5.4:+20599077 MS.gene027880:CDS
AATCTTTCTGATTATGGTGG+TGG 0.549706 5.4:+20599162 MS.gene027880:CDS
TCGTTAATCCAAATCGAAGA+AGG 0.550466 5.4:-20598883 None:intergenic
ATCATTTACCACAGACACAC+TGG 0.550984 5.4:-20600048 None:intergenic
TTCAAATTCGACGCCCCTGG+TGG 0.552724 5.4:-20599438 None:intergenic
TCGCCCTCTCAGCTTGTGAG+AGG 0.553021 5.4:+20598656 MS.gene027880:CDS
AAATTCTCTGGTGGTACTGG+TGG 0.553450 5.4:+20599459 MS.gene027880:CDS
GAACCTGAATAGTCGTAAGA+AGG 0.553990 5.4:-20598337 None:intergenic
TCGATGTTAAGATTATAGGC+TGG 0.555177 5.4:+20599931 MS.gene027880:CDS
GGAGGCTTCGGTGGTGAAGG+AGG 0.561351 5.4:+20599506 MS.gene027880:CDS
TGTGGTGGTTGTGACGGTGG+TGG 0.563353 5.4:-20598217 None:intergenic
ACGATGCAATTTGAAACTCC+TGG 0.564466 5.4:+20599057 MS.gene027880:CDS
GAATTATAATAAGCTTGTCC+AGG 0.566444 5.4:-20598601 None:intergenic
TTCGGATCTGGAGGCTTCGG+TGG 0.573741 5.4:+20599497 MS.gene027880:CDS
ATTAGAGCTTGATTCGAAGA+GGG 0.573791 5.4:-20599897 None:intergenic
TGTGACGGTGGTGGTGCTGG+TGG 0.575305 5.4:-20598208 None:intergenic
GAAGCCGGCAAACGGAAGAG+AGG 0.578591 5.4:+20599581 MS.gene027880:CDS
CAACCACCACAAACCACCAC+CGG 0.580431 5.4:+20598229 MS.gene027880:CDS
TGATGAAGAAGTTACTGATA+CGG 0.580523 5.4:+20598525 MS.gene027880:CDS
GCGGCATTGTCCTCTAAAGT+CGG 0.582204 5.4:+20600134 MS.gene027880:CDS
AGAAGCCGAGGAAGAGAGGG+AGG 0.582977 5.4:+20599559 MS.gene027880:CDS
ACAGTTAGTTTCCGTTGCTG+AGG 0.584195 5.4:+20599308 MS.gene027880:CDS
TCGCCCTCTCACAAGCTGAG+AGG 0.584834 5.4:-20598660 None:intergenic
CGTCTTCAAGCTTTAATCGA+AGG 0.585450 5.4:+20598277 MS.gene027880:CDS
CACTCAGGAGCAGCTTCGAG+CGG 0.590406 5.4:+20600115 MS.gene027880:CDS
TCAGCTTCAACATGATTCAA+CGG 0.591097 5.4:-20599606 None:intergenic
CAGAACAATAACAATAATCA+GGG 0.591184 5.4:+20599012 MS.gene027880:CDS
AATCCGGTGGTGGTTTGTGG+TGG 0.592017 5.4:-20598232 None:intergenic
GTTGCTGAGGAGAACAACAA+TGG 0.596228 5.4:+20599321 MS.gene027880:CDS
CCATTAACCTTGCTGCCGGG+TGG 0.597585 5.4:-20599989 None:intergenic
TGGTGTAATTGTTCCACCAG+GGG 0.598619 5.4:+20599425 MS.gene027880:CDS
CTGGTGTAATTGTTCCACCA+GGG 0.599733 5.4:+20599424 MS.gene027880:CDS
ACCAAAACACAAAATCTCAC+CGG 0.600817 5.4:-20599217 None:intergenic
CGATAACTCTTCAGTAATGG+AGG 0.601389 5.4:+20598150 MS.gene027880:CDS
TCCGTTAAAGTACTCGAACC+AGG 0.602105 5.4:-20599075 None:intergenic
ATTGTTGTTCTCCTCAGCAA+CGG 0.603161 5.4:-20599319 None:intergenic
TTCGGACCATTCGGATCTGG+AGG 0.603525 5.4:+20599488 MS.gene027880:CDS
GGTGGATAGTAGTCGTGTGG+TGG 0.606483 5.4:+20599527 MS.gene027880:CDS
GAGGAGAACAACAATGGGAA+CGG 0.611347 5.4:+20599327 MS.gene027880:CDS
TTAGGTAATTCTGCCCATCA+TGG 0.611875 5.4:+20598850 MS.gene027880:CDS
TCCAAAACACCGTCAGCCGA+TGG 0.615737 5.4:-20598724 None:intergenic
GATTAGAGCTTGATTCGAAG+AGG 0.620386 5.4:-20599898 None:intergenic
GCGAGACAAGGTCAAGAACA+TGG 0.625791 5.4:+20598679 MS.gene027880:CDS
AACAAGCAACCGTGAAAATG+GGG 0.625969 5.4:+20600081 MS.gene027880:CDS
GCTTGTGAGAGGGCGAGACA+AGG 0.630681 5.4:+20598667 MS.gene027880:CDS
TCGAATTTGAAATTCTCTGG+TGG 0.634544 5.4:+20599450 MS.gene027880:CDS
ATTATAATTCAGCTCCGGTG+TGG 0.637766 5.4:+20598614 MS.gene027880:CDS
CACCTGCTGAACAAGAACAC+CGG 0.639706 5.4:+20598437 MS.gene027880:CDS
GGCATTGTCCTCTAAAGTCG+GGG 0.642951 5.4:+20600136 MS.gene027880:CDS
GCGTGTATACCATCGGCTGA+CGG 0.644460 5.4:+20598715 MS.gene027880:CDS
AGGAGATCACCAAATTCGAG+AGG 0.645015 5.4:+20599366 MS.gene027880:CDS
GACGCTGGCGTGTATACCAT+CGG 0.650932 5.4:+20598708 MS.gene027880:CDS
TGAATCATGTTGAAGCTGAG+AGG 0.655301 5.4:+20599610 MS.gene027880:CDS
AGTGTCTTGGTTGAATCCGG+TGG 0.657627 5.4:-20598245 None:intergenic
TTACCACAGACACACTGGCA+TGG 0.658103 5.4:-20600043 None:intergenic
ATGAATCTTTCTGATTATGG+TGG 0.662194 5.4:+20599159 MS.gene027880:CDS
TCAGCTCCAGTTAACCACAC+CGG 0.665554 5.4:-20598628 None:intergenic
GCACCATGCCAGTGTGTCTG+TGG 0.666701 5.4:+20600040 MS.gene027880:CDS
AGGTTGCCAGAAAATAGCGT+AGG 0.667613 5.4:-20598317 None:intergenic
TAAGGTTGATAAATCAGAGG+AGG 0.672550 5.4:-20598182 None:intergenic
CCATAAGGTTGATAAATCAG+AGG 0.672761 5.4:-20598185 None:intergenic
AGAACATGGTATACAGACGC+TGG 0.675420 5.4:+20598693 MS.gene027880:CDS
GGAGGTGGATAGTAGTCGTG+TGG 0.676666 5.4:+20599524 MS.gene027880:CDS
CCGAAGCCTCCAGATCCGAA+TGG 0.681912 5.4:-20599494 None:intergenic
TCTTGTTACAACAACAACAA+CGG 0.685083 5.4:+20598939 MS.gene027880:CDS
TGGAGCCCGAAAAGAAGCCG+AGG 0.695389 5.4:+20599547 MS.gene027880:CDS
AAGAAGTTACTGATACGGAG+TGG 0.724771 5.4:+20598530 MS.gene027880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAACAATAATTTTGATTT+TGG + chr5.4:20598814-20598833 MS.gene027880:CDS 10.0%
!! AAAACTGAATCTAATAAAGA+TGG + chr5.4:20599765-20599784 MS.gene027880:CDS 20.0%
! ACAGAACAATAACAATAATC+AGG + chr5.4:20599011-20599030 MS.gene027880:CDS 25.0%
! AGTTCTTATGTTGCTAATAA+TGG + chr5.4:20599252-20599271 MS.gene027880:CDS 25.0%
! CAGAACAATAACAATAATCA+GGG + chr5.4:20599012-20599031 MS.gene027880:CDS 25.0%
! GAAATGAATCTTTCTGATTA+TGG + chr5.4:20599156-20599175 MS.gene027880:CDS 25.0%
! GAACTTAATTCATTAATCTC+CGG + chr5.4:20598475-20598494 MS.gene027880:CDS 25.0%
! GATATCGATGTTAAGATTAT+AGG + chr5.4:20599927-20599946 MS.gene027880:CDS 25.0%
!! TATTGTTATTGTTCTGTTGT+TGG - chr5.4:20599008-20599027 None:intergenic 25.0%
!! TGATCAAAGTTTCTTTTCTA+AGG + chr5.4:20599134-20599153 MS.gene027880:CDS 25.0%
!!! ATTTTGATTTTGGATCTTCT+TGG + chr5.4:20598824-20598843 MS.gene027880:CDS 25.0%
!!! TTATCCATCTTTGAAACATT+AGG - chr5.4:20599687-20599706 None:intergenic 25.0%
AGCTTATTATAATTCAGCTC+CGG + chr5.4:20598609-20598628 MS.gene027880:CDS 30.0%
ATGAATCTTTCTGATTATGG+TGG + chr5.4:20599159-20599178 MS.gene027880:CDS 30.0%
GAATTATAATAAGCTTGTCC+AGG - chr5.4:20598604-20598623 None:intergenic 30.0%
TCTTGTTACAACAACAACAA+CGG + chr5.4:20598939-20598958 MS.gene027880:CDS 30.0%
TGATGAAGAAGTTACTGATA+CGG + chr5.4:20598525-20598544 MS.gene027880:CDS 30.0%
TGTTCCTAATGTTTCAAAGA+TGG + chr5.4:20599680-20599699 MS.gene027880:CDS 30.0%
!! ATGGATAAAGCTTCACTTTT+GGG + chr5.4:20599699-20599718 MS.gene027880:CDS 30.0%
AATCTTTCTGATTATGGTGG+TGG + chr5.4:20599162-20599181 MS.gene027880:CDS 35.0%
ACCAAAACACAAAATCTCAC+CGG - chr5.4:20599220-20599239 None:intergenic 35.0%
AGAATGATCCTTCTTCGATT+TGG + chr5.4:20598875-20598894 MS.gene027880:CDS 35.0%
AGAGGAAGCAATAATGATGA+TGG + chr5.4:20599384-20599403 MS.gene027880:CDS 35.0%
CCTCTGATTTATCAACCTTA+TGG + chr5.4:20598185-20598204 MS.gene027880:CDS 35.0%
CGATGTTAAGATTATAGGCT+GGG + chr5.4:20599932-20599951 MS.gene027880:CDS 35.0%
CTTGAAACATGAACAACACT+AGG - chr5.4:20599105-20599124 None:intergenic 35.0%
TCAGCTTCAACATGATTCAA+CGG - chr5.4:20599609-20599628 None:intergenic 35.0%
TCAGTAACTTCTTCATCAAC+AGG - chr5.4:20598523-20598542 None:intergenic 35.0%
TCGAATTCGAATTTCTTCTC+TGG + chr5.4:20599279-20599298 MS.gene027880:CDS 35.0%
TCGATGTTAAGATTATAGGC+TGG + chr5.4:20599931-20599950 MS.gene027880:CDS 35.0%
TCGTTAATCCAAATCGAAGA+AGG - chr5.4:20598886-20598905 None:intergenic 35.0%
TGATTCGAAGAGGGTTTATT+TGG - chr5.4:20599891-20599910 None:intergenic 35.0%
TTAAAGCTTGAAGACGTTGT+TGG - chr5.4:20598273-20598292 None:intergenic 35.0%
TTATTGCTTCCTCTCGAATT+TGG - chr5.4:20599378-20599397 None:intergenic 35.0%
TTCATTAATCTCCGGTAATC+CGG + chr5.4:20598483-20598502 MS.gene027880:CDS 35.0%
! ATTAGAGCTTGATTCGAAGA+GGG - chr5.4:20599900-20599919 None:intergenic 35.0%
! GATGGATAAAGCTTCACTTT+TGG + chr5.4:20599698-20599717 MS.gene027880:CDS 35.0%
! GGAATGCTATCTTTTACTTC+TGG + chr5.4:20599405-20599424 MS.gene027880:CDS 35.0%
! TATTCAGGTTCTTCGTTACT+TGG + chr5.4:20598349-20598368 MS.gene027880:CDS 35.0%
! TCGAATTTGAAATTCTCTGG+TGG + chr5.4:20599450-20599469 MS.gene027880:CDS 35.0%
!! CCATAAGGTTGATAAATCAG+AGG - chr5.4:20598188-20598207 None:intergenic 35.0%
!! GTTCTCTAAGAACTTTTCTC+CGG - chr5.4:20598459-20598478 None:intergenic 35.0%
!! TAAGGTTGATAAATCAGAGG+AGG - chr5.4:20598185-20598204 None:intergenic 35.0%
AACAAGCAACCGTGAAAATG+GGG + chr5.4:20600081-20600100 MS.gene027880:CDS 40.0%
AAGAAGTTACTGATACGGAG+TGG + chr5.4:20598530-20598549 MS.gene027880:CDS 40.0%
AAGGATCATTCTCACCATGA+TGG - chr5.4:20598867-20598886 None:intergenic 40.0%
AAGGTTAATGGCAGCGTTAA+TGG + chr5.4:20600001-20600020 MS.gene027880:CDS 40.0%
ACAACAAGCAACCGTGAAAA+TGG + chr5.4:20600079-20600098 MS.gene027880:CDS 40.0%
ACAGGAGATTCTTCTGTAAC+CGG - chr5.4:20598505-20598524 None:intergenic 40.0%
AGGATCATTCTCACCATGAT+GGG - chr5.4:20598866-20598885 None:intergenic 40.0%
AGGTTCTTCGTTACTTGGTT+GGG + chr5.4:20598354-20598373 MS.gene027880:CDS 40.0%
ATCATTTACCACAGACACAC+TGG - chr5.4:20600051-20600070 None:intergenic 40.0%
ATGCAGTAAGAAAAACCACC+CGG + chr5.4:20599974-20599993 MS.gene027880:CDS 40.0%
ATTATAATTCAGCTCCGGTG+TGG + chr5.4:20598614-20598633 MS.gene027880:CDS 40.0%
CAACAAGCAACCGTGAAAAT+GGG + chr5.4:20600080-20600099 MS.gene027880:CDS 40.0%
CAACCTTCTTACGACTATTC+AGG + chr5.4:20598334-20598353 MS.gene027880:CDS 40.0%
CGACGATAACTCTTCAGTAA+TGG + chr5.4:20598147-20598166 MS.gene027880:CDS 40.0%
CGATAACTCTTCAGTAATGG+AGG + chr5.4:20598150-20598169 MS.gene027880:CDS 40.0%
GAACCTGAATAGTCGTAAGA+AGG - chr5.4:20598340-20598359 None:intergenic 40.0%
GAAGAGGGTTTATTTGGTAC+TGG - chr5.4:20599885-20599904 None:intergenic 40.0%
TGAATCATGTTGAAGCTGAG+AGG + chr5.4:20599610-20599629 MS.gene027880:CDS 40.0%
TTAGGTAATTCTGCCCATCA+TGG + chr5.4:20598850-20598869 MS.gene027880:CDS 40.0%
TTCTTCTGTAACCGGATTAC+CGG - chr5.4:20598497-20598516 None:intergenic 40.0%
TTGAAATTCTCTGGTGGTAC+TGG + chr5.4:20599456-20599475 MS.gene027880:CDS 40.0%
TTGCTGAGGAGAACAACAAT+GGG + chr5.4:20599322-20599341 MS.gene027880:CDS 40.0%
TTTGGATCTTCTTGGCAGTT+AGG + chr5.4:20598832-20598851 MS.gene027880:CDS 40.0%
! AAAACGACTCCCCATTTTCA+CGG - chr5.4:20600093-20600112 None:intergenic 40.0%
! ACGATGCAATTTGAAACTCC+TGG + chr5.4:20599057-20599076 MS.gene027880:CDS 40.0%
! AGTCTTTTGTCAACGGAACT+GGG + chr5.4:20598575-20598594 MS.gene027880:CDS 40.0%
! ATGACGCAGTCTTTTGTCAA+CGG + chr5.4:20598568-20598587 MS.gene027880:CDS 40.0%
! ATTGTTGTTCTCCTCAGCAA+CGG - chr5.4:20599322-20599341 None:intergenic 40.0%
! CGTCTTCAAGCTTTAATCGA+AGG + chr5.4:20598277-20598296 MS.gene027880:CDS 40.0%
! GATTAGAGCTTGATTCGAAG+AGG - chr5.4:20599901-20599920 None:intergenic 40.0%
! GCGTCGAATTTGAAATTCTC+TGG + chr5.4:20599447-20599466 MS.gene027880:CDS 40.0%
! GGTGGTTTTTCTTACTGCAT+TGG - chr5.4:20599974-20599993 None:intergenic 40.0%
! TCAACAGAATCTCTAGCTTC+AGG - chr5.4:20598910-20598929 None:intergenic 40.0%
! TCCGGTGAGATTTTGTGTTT+TGG + chr5.4:20599216-20599235 MS.gene027880:CDS 40.0%
! TCCTGGTTCGAGTACTTTAA+CGG + chr5.4:20599074-20599093 MS.gene027880:CDS 40.0%
! TGGGGTGATGGTTACTATAA+AGG + chr5.4:20598373-20598392 MS.gene027880:CDS 40.0%
!! TGTTGTTGTTGCTGCTGTTT+TGG - chr5.4:20599861-20599880 None:intergenic 40.0%
AAAAGAAGCCGAGGAAGAGA+GGG + chr5.4:20599556-20599575 MS.gene027880:CDS 45.0%
AACAACAATGGGAACGGGAA+TGG + chr5.4:20599333-20599352 MS.gene027880:CDS 45.0%
AATTTCAAATTCGACGCCCC+TGG - chr5.4:20599444-20599463 None:intergenic 45.0%
ACAACAATGGGAACGGGAAT+GGG + chr5.4:20599334-20599353 MS.gene027880:CDS 45.0%
AGAACATGGTATACAGACGC+TGG + chr5.4:20598693-20598712 MS.gene027880:CDS 45.0%
AGGAGAACAACAATGGGAAC+GGG + chr5.4:20599328-20599347 MS.gene027880:CDS 45.0%
AGGAGATCACCAAATTCGAG+AGG + chr5.4:20599366-20599385 MS.gene027880:CDS 45.0%
AGGCTGGGATGCAATGATAA+GGG + chr5.4:20599947-20599966 MS.gene027880:CDS 45.0%
AGGTTGCCAGAAAATAGCGT+AGG - chr5.4:20598320-20598339 None:intergenic 45.0%
CACAAAATCTCACCGGATTC+CGG - chr5.4:20599213-20599232 None:intergenic 45.0%
CAGGTTCTTCGTTACTTGGT+TGG + chr5.4:20598353-20598372 MS.gene027880:CDS 45.0%
CTGGTGTAATTGTTCCACCA+GGG + chr5.4:20599424-20599443 MS.gene027880:CDS 45.0%
GAGGAGAACAACAATGGGAA+CGG + chr5.4:20599327-20599346 MS.gene027880:CDS 45.0%
GGTTCTTCGTTACTTGGTTG+GGG + chr5.4:20598355-20598374 MS.gene027880:CDS 45.0%
GTTGCTGAGGAGAACAACAA+TGG + chr5.4:20599321-20599340 MS.gene027880:CDS 45.0%
TAGGCTGGGATGCAATGATA+AGG + chr5.4:20599946-20599965 MS.gene027880:CDS 45.0%
TCTGGTGTAATTGTTCCACC+AGG + chr5.4:20599423-20599442 MS.gene027880:CDS 45.0%
TGGTGTAATTGTTCCACCAG+GGG + chr5.4:20599425-20599444 MS.gene027880:CDS 45.0%
TGGTTCGAGTACTTTAACGG+AGG + chr5.4:20599077-20599096 MS.gene027880:CDS 45.0%
TTGAACATCCCCGACTTTAG+AGG - chr5.4:20600147-20600166 None:intergenic 45.0%
! AAATTCTCTGGTGGTACTGG+TGG + chr5.4:20599459-20599478 MS.gene027880:CDS 45.0%
! ACAGTTAGTTTCCGTTGCTG+AGG + chr5.4:20599308-20599327 MS.gene027880:CDS 45.0%
! CAGTCTTTTGTCAACGGAAC+TGG + chr5.4:20598574-20598593 MS.gene027880:CDS 45.0%
! TCAGCAACGTTTGTTGAAGC+CGG + chr5.4:20599191-20599210 MS.gene027880:CDS 45.0%
! TCCGTTAAAGTACTCGAACC+AGG - chr5.4:20599078-20599097 None:intergenic 45.0%
! TCTCTTCCTCGGCTTCTTTT+CGG - chr5.4:20599556-20599575 None:intergenic 45.0%
!! GAGAGTGTCTTGGTTGAATC+CGG - chr5.4:20598251-20598270 None:intergenic 45.0%
!! TCTGGACCTACGCTATTTTC+TGG + chr5.4:20598311-20598330 MS.gene027880:CDS 45.0%
!!! TGTTGTTGCTGCTGTTTTGG+TGG - chr5.4:20599858-20599877 None:intergenic 45.0%
AATGGGAACGGGAATGGGAA+TGG + chr5.4:20599339-20599358 MS.gene027880:CDS 50.0%
ACGGGAATGGGAATGGAAAG+AGG + chr5.4:20599346-20599365 MS.gene027880:CDS 50.0%
AGTGTCTTGGTTGAATCCGG+TGG - chr5.4:20598248-20598267 None:intergenic 50.0%
CACCTGCTGAACAAGAACAC+CGG + chr5.4:20598437-20598456 MS.gene027880:CDS 50.0%
GAAAAGAAGCCGAGGAAGAG+AGG + chr5.4:20599555-20599574 MS.gene027880:CDS 50.0%
GCGAGACAAGGTCAAGAACA+TGG + chr5.4:20598679-20598698 MS.gene027880:CDS 50.0%
TCAGCTCCAGTTAACCACAC+CGG - chr5.4:20598631-20598650 None:intergenic 50.0%
TCGTTACTTGGTTGGGGTGA+TGG + chr5.4:20598361-20598380 MS.gene027880:CDS 50.0%
TTACCACAGACACACTGGCA+TGG - chr5.4:20600046-20600065 None:intergenic 50.0%
! CGTTTGTTGAAGCCGGAATC+CGG + chr5.4:20599198-20599217 MS.gene027880:CDS 50.0%
! CTCTTCCTCGGCTTCTTTTC+GGG - chr5.4:20599555-20599574 None:intergenic 50.0%
! GTGGTTTGTGGTGGTTGTGA+CGG - chr5.4:20598226-20598245 None:intergenic 50.0%
! TCGAAGGCGCAAAAGAAGTC+TGG + chr5.4:20598293-20598312 MS.gene027880:CDS 50.0%
! TGATTCGGACCATTCGGATC+TGG + chr5.4:20599485-20599504 MS.gene027880:CDS 50.0%
! TGGTGGTGATTCGGACCATT+CGG + chr5.4:20599479-20599498 MS.gene027880:CDS 50.0%
! TTGAATCCGGTGGTGGTTTG+TGG - chr5.4:20598238-20598257 None:intergenic 50.0%
!! CGGCATTGTCCTCTAAAGTC+GGG + chr5.4:20600135-20600154 MS.gene027880:CDS 50.0%
!! GACGTTGTTGGAGAGTGTCT+TGG - chr5.4:20598261-20598280 None:intergenic 50.0%
!! GCGGCATTGTCCTCTAAAGT+CGG + chr5.4:20600134-20600153 MS.gene027880:CDS 50.0%
!! GGCATTGTCCTCTAAAGTCG+GGG + chr5.4:20600136-20600155 MS.gene027880:CDS 50.0%
!! GTTTTGGTGGCTGATGAGAC+TGG - chr5.4:20599845-20599864 None:intergenic 50.0%
!! TGGTACTGGTGGTGGTGATT+CGG + chr5.4:20599470-20599489 MS.gene027880:CDS 50.0%
!! TGTTCTGTTGTTGGTGCTGC+TGG - chr5.4:20598999-20599018 None:intergenic 50.0%
!!! GGGGAGTCGTTTTTACACTC+AGG + chr5.4:20600100-20600119 MS.gene027880:CDS 50.0%
AGAAAAACCACCCGGCAGCA+AGG + chr5.4:20599982-20600001 MS.gene027880:CDS 55.0%
CAACCACCACAAACCACCAC+CGG + chr5.4:20598229-20598248 MS.gene027880:CDS 55.0%
CACTGGCATGGTGCACTTCT+AGG - chr5.4:20600034-20600053 None:intergenic 55.0%
CCATTCGGATCTGGAGGCTT+CGG + chr5.4:20599494-20599513 MS.gene027880:CDS 55.0%
CTCCGGTGTTCTTGTTCAGC+AGG - chr5.4:20598442-20598461 None:intergenic 55.0%
CTGCCATTAACCTTGCTGCC+GGG - chr5.4:20599995-20600014 None:intergenic 55.0%
GACGCTGGCGTGTATACCAT+CGG + chr5.4:20598708-20598727 MS.gene027880:CDS 55.0%
GCTGCCATTAACCTTGCTGC+CGG - chr5.4:20599996-20600015 None:intergenic 55.0%
GGAGGTGGATAGTAGTCGTG+TGG + chr5.4:20599524-20599543 MS.gene027880:CDS 55.0%
GGTGGATAGTAGTCGTGTGG+TGG + chr5.4:20599527-20599546 MS.gene027880:CDS 55.0%
GGTTCCTCTCTTCCGTTTGC+CGG - chr5.4:20599588-20599607 None:intergenic 55.0%
GTCTTGGTTGAATCCGGTGG+TGG - chr5.4:20598245-20598264 None:intergenic 55.0%
GTTTGTGGTGGTTGTGACGG+TGG - chr5.4:20598223-20598242 None:intergenic 55.0%
TCAGCTCCGGTGTGGTTAAC+TGG + chr5.4:20598622-20598641 MS.gene027880:CDS 55.0%
TCCAAAACACCGTCAGCCGA+TGG - chr5.4:20598727-20598746 None:intergenic 55.0%
TTCAAATTCGACGCCCCTGG+TGG - chr5.4:20599441-20599460 None:intergenic 55.0%
TTCGGACCATTCGGATCTGG+AGG + chr5.4:20599488-20599507 MS.gene027880:CDS 55.0%
! AATCCGGTGGTGGTTTGTGG+TGG - chr5.4:20598235-20598254 None:intergenic 55.0%
! GCGTGTATACCATCGGCTGA+CGG + chr5.4:20598715-20598734 MS.gene027880:CDS 55.0%
!! ACCATCGGCTGACGGTGTTT+TGG + chr5.4:20598723-20598742 MS.gene027880:CDS 55.0%
!! TGTTGGTGCTGCTGGTGACT+TGG - chr5.4:20598991-20599010 None:intergenic 55.0%
!! TTCTCTGGTGGTACTGGTGG+TGG + chr5.4:20599462-20599481 MS.gene027880:CDS 55.0%
!!! GCTGACGGTGTTTTGGAGCT+TGG + chr5.4:20598730-20598749 MS.gene027880:CDS 55.0%
AGAAGCCGAGGAAGAGAGGG+AGG + chr5.4:20599559-20599578 MS.gene027880:CDS 60.0%
AGAGGGAGGAAGCCGGCAAA+CGG + chr5.4:20599573-20599592 MS.gene027880:CDS 60.0%
CACTCAGGAGCAGCTTCGAG+CGG + chr5.4:20600115-20600134 MS.gene027880:CDS 60.0%
CCACCCGGCAGCAAGGTTAA+TGG + chr5.4:20599989-20600008 MS.gene027880:CDS 60.0%
CCATTAACCTTGCTGCCGGG+TGG - chr5.4:20599992-20600011 None:intergenic 60.0%
CCGAAGCCTCCAGATCCGAA+TGG - chr5.4:20599497-20599516 None:intergenic 60.0%
CGCCCTCTCACAAGCTGAGA+GGG - chr5.4:20598662-20598681 None:intergenic 60.0%
CGCCCTCTCAGCTTGTGAGA+GGG + chr5.4:20598657-20598676 MS.gene027880:CDS 60.0%
GAAGCCGGCAAACGGAAGAG+AGG + chr5.4:20599581-20599600 MS.gene027880:CDS 60.0%
GAGGAAGAGAGGGAGGAAGC+CGG + chr5.4:20599566-20599585 MS.gene027880:CDS 60.0%
GCACCATGCCAGTGTGTCTG+TGG + chr5.4:20600040-20600059 MS.gene027880:CDS 60.0%
GCTTGTGAGAGGGCGAGACA+AGG + chr5.4:20598667-20598686 MS.gene027880:CDS 60.0%
GGCTTCCTCCCTCTCTTCCT+CGG - chr5.4:20599567-20599586 None:intergenic 60.0%
TCGCCCTCTCACAAGCTGAG+AGG - chr5.4:20598663-20598682 None:intergenic 60.0%
TCGCCCTCTCAGCTTGTGAG+AGG + chr5.4:20598656-20598675 MS.gene027880:CDS 60.0%
TCTGGAGGCTTCGGTGGTGA+AGG + chr5.4:20599503-20599522 MS.gene027880:CDS 60.0%
TGGAGCCCGAAAAGAAGCCG+AGG + chr5.4:20599547-20599566 MS.gene027880:CDS 60.0%
TGTGGTGGTTGTGACGGTGG+TGG - chr5.4:20598220-20598239 None:intergenic 60.0%
TTCGGATCTGGAGGCTTCGG+TGG + chr5.4:20599497-20599516 MS.gene027880:CDS 60.0%
!! TGGTGGTGCTGGTGGCCATA+AGG - chr5.4:20598203-20598222 None:intergenic 60.0%
GGAGGCTTCGGTGGTGAAGG+AGG + chr5.4:20599506-20599525 MS.gene027880:CDS 65.0%
GGCTTCGGTGGTGAAGGAGG+TGG + chr5.4:20599509-20599528 MS.gene027880:CDS 65.0%
GTCAACGGAACTGGGCTGCC+TGG + chr5.4:20598583-20598602 MS.gene027880:CDS 65.0%
! GGTTGTGACGGTGGTGGTGC+TGG - chr5.4:20598214-20598233 None:intergenic 65.0%
! TGTGACGGTGGTGGTGCTGG+TGG - chr5.4:20598211-20598230 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 20598133 20600169 20598133 ID=MS.gene027880
chr5.4 mRNA 20598133 20600169 20598133 ID=MS.gene027880.t1;Parent=MS.gene027880
chr5.4 exon 20598133 20600169 20598133 ID=MS.gene027880.t1.exon1;Parent=MS.gene027880.t1
chr5.4 CDS 20598133 20600169 20598133 ID=cds.MS.gene027880.t1;Parent=MS.gene027880.t1
Gene Sequence

>MS.gene027880

ATGAATCTTTGGAGCGACGATAACTCTTCAGTAATGGAGGCTTTCATGACCTCCTCTGATTTATCAACCTTATGGCCACCAGCACCACCACCGTCACAACCACCACAAACCACCACCGGATTCAACCAAGACACTCTCCAACAACGTCTTCAAGCTTTAATCGAAGGCGCAAAAGAAGTCTGGACCTACGCTATTTTCTGGCAACCTTCTTACGACTATTCAGGTTCTTCGTTACTTGGTTGGGGTGATGGTTACTATAAAGGCGAAGAAGACAAAACAAAAGCGAAGAAATCAAAAGTTACTTCACCTGCTGAACAAGAACACCGGAGAAAAGTTCTTAGAGAACTTAATTCATTAATCTCCGGTAATCCGGTTACAGAAGAATCTCCTGTTGATGAAGAAGTTACTGATACGGAGTGGTTCTTTTTAGTTTCTATGACGCAGTCTTTTGTCAACGGAACTGGGCTGCCTGGACAAGCTTATTATAATTCAGCTCCGGTGTGGTTAACTGGAGCTGAAAATCTCGCCCTCTCAGCTTGTGAGAGGGCGAGACAAGGTCAAGAACATGGTATACAGACGCTGGCGTGTATACCATCGGCTGACGGTGTTTTGGAGCTTGGATCTACTGAGTTGATTTATCAGAATAATGATTTGATGAATAAAGTGAAGATGCTTTTCAATTTTAACAATAATTTTGATTTTGGATCTTCTTGGCAGTTAGGTAATTCTGCCCATCATGGTGAGAATGATCCTTCTTCGATTTGGATTAACGATCCTGAAGCTAGAGATTCTGTTGATAATAATTCTCTTGTTACAACAACAACAACGGCAGCAACAAATGCTTCAATTTCAATTCCAAGTCACCAGCAGCACCAACAACAGAACAATAACAATAATCAGGGTTTGAGTGTGAGTGTGACGAAGACGATGCAATTTGAAACTCCTGGTTCGAGTACTTTAACGGAGGTTCCTAGTGTTGTTCATGTTTCAAGCAAGCAGAATGATCAAAGTTTCTTTTCTAAGGAAATGAATCTTTCTGATTATGGTGGTGGTAACAATCAGCAACGTTTGTTGAAGCCGGAATCCGGTGAGATTTTGTGTTTTGGTGAGAGTAAAAAGAGTTCTTATGTTGCTAATAATGGAAACTCGAATTCGAATTTCTTCTCTGGTCAATCACAGTTAGTTTCCGTTGCTGAGGAGAACAACAATGGGAACGGGAATGGGAATGGAAAGAGGAGATCACCAAATTCGAGAGGAAGCAATAATGATGATGGAATGCTATCTTTTACTTCTGGTGTAATTGTTCCACCAGGGGCGTCGAATTTGAAATTCTCTGGTGGTACTGGTGGTGGTGATTCGGACCATTCGGATCTGGAGGCTTCGGTGGTGAAGGAGGTGGATAGTAGTCGTGTGGTGGAGCCCGAAAAGAAGCCGAGGAAGAGAGGGAGGAAGCCGGCAAACGGAAGAGAGGAACCGTTGAATCATGTTGAAGCTGAGAGGCAAAGAAGAGAGAAGCTGAATCAAAGATTCTATGCACTTCGAGCAGTTGTTCCTAATGTTTCAAAGATGGATAAAGCTTCACTTTTGGGTGATGCTATATCTTACATTACTGAGTTGAAAACAAAGCTTCAGAAAACTGAATCTAATAAAGATGGATTAGAAAAGCAACTTGATGCGATGAAGAATGAGATTCAGAAAATTAACGAAAACCAGTCTCATCAGCCACCAAAACAGCAGCAACAACAACAGCCAGTACCAAATAAACCCTCTTCGAATCAAGCTCTAATCGATTTAGATATCGATGTTAAGATTATAGGCTGGGATGCAATGATAAGGGTCCAATGCAGTAAGAAAAACCACCCGGCAGCAAGGTTAATGGCAGCGTTAATGGAGCTTGACCTAGAAGTGCACCATGCCAGTGTGTCTGTGGTAAATGATTTGATGATACAACAAGCAACCGTGAAAATGGGGAGTCGTTTTTACACTCAGGAGCAGCTTCGAGCGGCATTGTCCTCTAAAGTCGGGGATGTTCAATAA
Protein sequence

>MS.gene027880.t1

MNLWSDDNSSVMEAFMTSSDLSTLWPPAPPPSQPPQTTTGFNQDTLQQRLQALIEGAKEVWTYAIFWQPSYDYSGSSLLGWGDGYYKGEEDKTKAKKSKVTSPAEQEHRRKVLRELNSLISGNPVTEESPVDEEVTDTEWFFLVSMTQSFVNGTGLPGQAYYNSAPVWLTGAENLALSACERARQGQEHGIQTLACIPSADGVLELGSTELIYQNNDLMNKVKMLFNFNNNFDFGSSWQLGNSAHHGENDPSSIWINDPEARDSVDNNSLVTTTTTAATNASISIPSHQQHQQQNNNNNQGLSVSVTKTMQFETPGSSTLTEVPSVVHVSSKQNDQSFFSKEMNLSDYGGGNNQQRLLKPESGEILCFGESKKSSYVANNGNSNSNFFSGQSQLVSVAEENNNGNGNGNGKRRSPNSRGSNNDDGMLSFTSGVIVPPGASNLKFSGGTGGGDSDHSDLEASVVKEVDSSRVVEPEKKPRKRGRKPANGREEPLNHVEAERQRREKLNQRFYALRAVVPNVSKMDKASLLGDAISYITELKTKLQKTESNKDGLEKQLDAMKNEIQKINENQSHQPPKQQQQQQPVPNKPSSNQALIDLDIDVKIIGWDAMIRVQCSKKNHPAARLMAALMELDLEVHHASVSVVNDLMIQQATVKMGSRFYTQEQLRAALSSKVGDVQ