AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028027

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028027.t1 MTR_5g027570 69.333 225 48 5 116 323 11 231 1.72e-95 283
MS.gene028027.t1 MTR_5g088060 44.156 462 87 15 20 323 13 461 3.72e-87 269
MS.gene028027.t1 MTR_5g488160 44.156 462 87 15 20 323 13 461 3.72e-87 269
MS.gene028027.t1 MTR_5g027550 61.702 235 47 7 116 323 11 229 1.55e-77 237
MS.gene028027.t1 MTR_5g088010 50.584 257 52 7 134 323 1 249 1.71e-64 204
MS.gene028027.t1 MTR_5g027780 70.000 140 30 2 20 149 36 173 2.51e-56 181
MS.gene028027.t1 MTR_5g027770 49.776 223 38 7 1 202 1 170 8.05e-48 163
MS.gene028027.t1 MTR_5g027770 58.015 131 17 2 21 115 171 299 5.28e-35 129
MS.gene028027.t1 MTR_5g088200 49.727 183 24 4 1 115 1 183 1.11e-40 151
MS.gene028027.t1 MTR_5g088005 58.915 129 29 3 1 115 1 119 1.30e-35 127
MS.gene028027.t1 MTR_3g462790 58.333 84 30 3 194 274 143 224 4.19e-21 92.4
MS.gene028027.t1 MTR_1g111830 44.262 122 60 4 166 282 89 207 1.58e-20 89.4
MS.gene028027.t1 MTR_8g063600 54.217 83 35 2 194 274 102 183 2.99e-20 89.4
MS.gene028027.t1 MTR_5g081860 54.348 92 35 3 182 270 113 200 4.52e-20 88.6
MS.gene028027.t1 MTR_4g100630 37.086 151 73 6 148 292 47 181 1.55e-19 87.8
MS.gene028027.t1 MTR_2g100930 47.368 95 46 2 194 286 105 197 1.87e-19 88.2
MS.gene028027.t1 MTR_5g069710 54.444 90 36 3 194 280 128 215 2.59e-19 87.0
MS.gene028027.t1 MTR_4g111975 51.250 80 34 3 194 270 134 211 1.73e-18 84.7
MS.gene028027.t1 MTR_8g063870 52.273 88 37 3 191 274 83 169 2.95e-18 83.6
MS.gene028027.t1 MTR_7g067080 57.534 73 27 2 194 264 136 206 7.98e-18 82.8
MS.gene028027.t1 MTR_1g048660 54.795 73 29 2 200 270 14 84 8.33e-17 74.7
MS.gene028027.t1 MTR_4g107230 43.802 121 56 5 181 298 66 177 2.83e-16 78.2
MS.gene028027.t1 MTR_3g104370 40.496 121 57 5 171 280 67 183 3.83e-16 76.6
MS.gene028027.t1 MTR_8g101650 54.286 70 27 2 194 261 101 167 1.28e-15 76.3
MS.gene028027.t1 MTR_6g092540 54.286 70 28 2 194 261 93 160 1.81e-15 75.9
MS.gene028027.t1 MTR_5g037080 62.963 54 18 1 192 243 96 149 3.42e-14 72.4
MS.gene028027.t1 MTR_1g083180 50.633 79 33 3 195 269 58 134 9.24e-14 68.9
MS.gene028027.t1 MTR_4g015130 50.725 69 29 3 208 273 3 69 9.33e-14 66.2
MS.gene028027.t1 MTR_5g075760 45.977 87 39 4 199 282 106 187 1.24e-13 69.3
MS.gene028027.t1 MTR_5g075790 34.437 151 81 8 140 282 55 195 1.35e-12 66.6
MS.gene028027.t1 MTR_3g070110 52.778 72 27 2 199 263 96 167 7.74e-12 64.3
MS.gene028027.t1 MTR_1g033620 54.098 61 23 3 213 270 20 78 8.30e-12 64.7
MS.gene028027.t1 MTR_0036s0260 43.478 69 35 2 200 266 30 96 9.54e-12 63.9
MS.gene028027.t1 MTR_2g090305 44.928 69 34 2 200 266 30 96 1.74e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028027.t1 AT5G08520 46.535 101 50 2 165 263 85 183 5.00e-21 91.7
MS.gene028027.t1 AT5G58900 61.333 75 24 3 194 265 137 209 3.68e-20 89.0
MS.gene028027.t1 AT1G49010 49.451 91 41 3 190 277 126 214 1.49e-19 87.8
MS.gene028027.t1 AT5G04760 53.012 83 35 2 183 263 84 164 1.97e-19 85.5
MS.gene028027.t1 AT2G38090 47.748 111 47 5 168 269 105 213 1.02e-18 85.1
MS.gene028027.t1 AT5G23650 43.590 117 59 3 158 272 83 194 2.28e-18 84.7
MS.gene028027.t1 AT5G61620 48.515 101 36 4 187 277 101 195 6.52e-18 83.2
MS.gene028027.t1 AT5G01200 56.944 72 27 2 194 263 142 211 1.92e-17 81.3
MS.gene028027.t1 AT3G11280 51.111 90 36 3 184 267 113 200 2.30e-17 80.9
MS.gene028027.t1 AT3G11280 51.111 90 36 3 184 267 113 200 2.30e-17 80.9
MS.gene028027.t1 AT5G05790 45.455 99 49 3 183 278 116 212 6.04e-17 80.1
MS.gene028027.t1 AT5G05790 45.455 99 49 3 183 278 116 212 6.04e-17 80.1
MS.gene028027.t1 AT3G10580 43.878 98 46 4 182 275 77 169 1.24e-16 78.6
MS.gene028027.t1 AT5G47390 51.351 74 32 2 190 261 88 159 1.27e-16 80.1
MS.gene028027.t1 AT3G10580 43.878 98 46 4 182 275 77 169 1.74e-16 79.0
MS.gene028027.t1 AT5G56840 55.714 70 28 2 194 261 87 155 1.58e-15 75.1
MS.gene028027.t1 AT3G10590 44.444 108 50 5 181 284 92 193 2.34e-15 73.9
MS.gene028027.t1 AT1G74840 57.812 64 25 1 181 242 79 142 6.44e-15 73.6
MS.gene028027.t1 AT1G74840 57.812 64 25 1 181 242 79 142 7.16e-15 73.9
MS.gene028027.t1 AT4G09450 52.703 74 31 2 193 264 86 157 1.83e-14 71.6
MS.gene028027.t1 AT3G16350 50.685 73 34 1 173 243 112 184 3.23e-14 73.2
MS.gene028027.t1 AT1G19000 65.385 52 16 1 193 242 97 148 3.35e-14 72.0
MS.gene028027.t1 AT1G19000 65.385 52 16 1 193 242 97 148 3.35e-14 72.0
MS.gene028027.t1 AT1G70000 61.111 54 19 1 192 243 92 145 7.28e-14 70.9
MS.gene028027.t1 AT1G70000 61.111 54 19 1 192 243 92 145 7.28e-14 70.9

Find 81 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTGTATCTTTCACATTT+TGG 0.106986 5.3:-17221299 None:intergenic
TGATGTGTCATTCTTGTTTA+AGG 0.162194 5.3:-17221355 None:intergenic
CTCCATCAAAATGATTTAAA+TGG 0.178664 5.3:+17220372 MS.gene028027:CDS
TGTGATGTATAGGTTGTTTC+TGG 0.210646 5.3:+17221173 MS.gene028027:intron
TCAACATGATTCTTAGGTTT+TGG 0.214289 5.3:-17219741 None:intergenic
ACATTCTCAACATGATTCTT+AGG 0.253797 5.3:-17219747 None:intergenic
TTCTCAAGTTTGTTCATATC+AGG 0.295923 5.3:-17221477 None:intergenic
TTTGCTTCATGACTTCCTTC+TGG 0.307695 5.3:-17220579 None:intergenic
TATGCTTTCCCACCTGGTTC+AGG 0.329227 5.3:+17219867 MS.gene028027:CDS
GCGCAACTTGTTCAACTTGT+TGG 0.354873 5.3:+17221503 MS.gene028027:CDS
CCTGCATATGCTTTCCCACC+TGG 0.358776 5.3:+17219861 MS.gene028027:CDS
GATGTTCCTGTGTTGGTTGT+TGG 0.372353 5.3:+17219681 MS.gene028027:CDS
TGCTATGCTCAGAGGGATCT+TGG 0.382368 5.3:-17220407 None:intergenic
CGAACATTGCGCTTGGGGCC+TGG 0.388404 5.3:-17219801 None:intergenic
GGATTCTCACCATTCGGATA+AGG 0.417277 5.3:-17219891 None:intergenic
GGCTTAGGATTCTCACCATT+CGG 0.437848 5.3:-17219897 None:intergenic
ATGACCTGACTCACTGATAT+CGG 0.439498 5.3:-17220457 None:intergenic
CGAAGACGAACATTGCGCTT+GGG 0.439662 5.3:-17219807 None:intergenic
GAACAACAACAGGAGAAAAC+TGG 0.441684 5.3:+17220432 MS.gene028027:CDS
TAGGTTGTTTCTGGAGGGGC+TGG 0.445563 5.3:+17221182 MS.gene028027:intron
TCCATCAAAATGATTTAAAT+GGG 0.446307 5.3:+17220373 MS.gene028027:CDS
CACATCAGTCACTGCTCCTA+TGG 0.448672 5.3:+17221371 MS.gene028027:CDS
GGTTTCTTCCATGTAGACTT+AGG 0.456119 5.3:-17219636 None:intergenic
AACTTAGTTCAAGCTGAGTC+AGG 0.458703 5.3:+17220510 MS.gene028027:CDS
CCTACTGCCACCGATGATAC+AGG 0.473482 5.3:-17219918 None:intergenic
GGCACCGATATCAGTGAGTC+AGG 0.482648 5.3:+17220453 MS.gene028027:CDS
GAGGGATCTTGGACTGAAGC+TGG 0.485915 5.3:-17220396 None:intergenic
GCATAGCATGGAACAACAAC+AGG 0.485938 5.3:+17220422 MS.gene028027:CDS
TTGGTGCTGCTCTGTCCAAT+CGG 0.496082 5.3:+17221444 MS.gene028027:CDS
TGATAGCGTGGTGATGCCAC+AGG 0.503741 5.3:-17219774 None:intergenic
CTTGAGGTGGCTGTGTCTTG+AGG 0.504065 5.3:-17221411 None:intergenic
GGATTCTCATGTTGTTCCAT+AGG 0.504821 5.3:-17221387 None:intergenic
CACCAACTGCATTTGTCTTG+AGG 0.508085 5.3:-17221427 None:intergenic
ACGAAGACGAACATTGCGCT+TGG 0.518066 5.3:-17219808 None:intergenic
ATGTGAGTTGGAGGTGCCAC+AGG 0.521985 5.3:-17219831 None:intergenic
GCATGGCTAGCAATTTGAGT+TGG 0.525594 5.3:-17221258 None:intergenic
AAAGAGAGGGCGTAAGGAAA+GGG 0.530997 5.3:+17220599 MS.gene028027:CDS
CAAAGAGAGGGCGTAAGGAA+AGG 0.532803 5.3:+17220598 MS.gene028027:CDS
CAAGCTGAGTCAGGAGCAGA+AGG 0.534197 5.3:+17220519 MS.gene028027:CDS
TGTTCCATGCTATGCTCAGA+GGG 0.536332 5.3:-17220414 None:intergenic
ATGTATAGGTTGTTTCTGGA+GGG 0.542704 5.3:+17221177 MS.gene028027:intron
GAATCCTAAGCCTGTATCAT+CGG 0.543820 5.3:+17219908 MS.gene028027:CDS
AGAATGAGCTGGCCAAGACT+TGG 0.545496 5.3:-17219706 None:intergenic
CAACTGCATTTGTCTTGAGG+TGG 0.545901 5.3:-17221424 None:intergenic
ATTAGAGTTTAGAATTGTGA+CGG 0.550391 5.3:-17220481 None:intergenic
GCAGGAACAGCAATGTGAGT+TGG 0.551521 5.3:-17219843 None:intergenic
TGGTTGTTGGAACCAAGTCT+TGG 0.553347 5.3:+17219694 MS.gene028027:CDS
GAAGACGAACATTGCGCTTG+GGG 0.560925 5.3:-17219806 None:intergenic
TTCGGATAAGGTCCTGAACC+AGG 0.561660 5.3:-17219879 None:intergenic
GCCACCGATGATACAGGCTT+AGG 0.564505 5.3:-17219912 None:intergenic
TCACCACGCTATCAAAAGCC+AGG 0.565310 5.3:+17219783 MS.gene028027:CDS
CCTGTATCATCGGTGGCAGT+AGG 0.567039 5.3:+17219918 MS.gene028027:CDS
TTGTTCCATGCTATGCTCAG+AGG 0.567759 5.3:-17220415 None:intergenic
CACCTCAAGACAAATGCAGT+TGG 0.567812 5.3:+17221425 MS.gene028027:CDS
TGAAGCAAAGAGAGGGCGTA+AGG 0.567823 5.3:+17220593 MS.gene028027:CDS
GAGGTGGCTGTGTCTTGAGG+AGG 0.576603 5.3:-17221408 None:intergenic
CAATGTTCGTCTTCGTCCTG+TGG 0.578341 5.3:+17219815 MS.gene028027:CDS
TCAGGAAGTGTAGAACCGAT+TGG 0.579196 5.3:-17221459 None:intergenic
AAGTGTTGATGTTCCTGTGT+TGG 0.580284 5.3:+17219674 MS.gene028027:CDS
GAAAGGGTACATTGGACAGA+AGG 0.584479 5.3:+17220615 MS.gene028027:CDS
GGCGTAAGGAAAGGGTACAT+TGG 0.585151 5.3:+17220607 MS.gene028027:CDS
TTAGTGCTCCTAAGTCTACA+TGG 0.585666 5.3:+17219628 MS.gene028027:CDS
CCAGGTGGGAAAGCATATGC+AGG 0.588904 5.3:-17219861 None:intergenic
GTTTGAGCATCAGAATGAGC+TGG 0.591524 5.3:-17219717 None:intergenic
TGTATAGGTTGTTTCTGGAG+GGG 0.594263 5.3:+17221178 MS.gene028027:intron
TCTTGTTTAAGGTTGTGTCG+TGG 0.595697 5.3:-17221344 None:intergenic
AGATCCCTCTGAGCATAGCA+TGG 0.596307 5.3:+17220410 MS.gene028027:CDS
ATGTGAAAGATACAGAGAAG+AGG 0.601303 5.3:+17221304 MS.gene028027:CDS
TCATCGGTGGCAGTAGGACA+AGG 0.602995 5.3:+17219924 MS.gene028027:CDS
GATGTATAGGTTGTTTCTGG+AGG 0.607647 5.3:+17221176 MS.gene028027:intron
GGAACAGCAATGTGAGTTGG+AGG 0.613590 5.3:-17219840 None:intergenic
AAGTCTACATGGAAGAAACC+TGG 0.626539 5.3:+17219639 MS.gene028027:CDS
GAAGTCATGAAGCAAAGAGA+GGG 0.628581 5.3:+17220586 MS.gene028027:CDS
GGAAGTCATGAAGCAAAGAG+AGG 0.629765 5.3:+17220585 MS.gene028027:CDS
CAAGCTGAGTCAGTACCAGA+AGG 0.630435 5.3:+17220564 MS.gene028027:CDS
TCCTAAGCCTGTATCATCGG+TGG 0.640182 5.3:+17219911 MS.gene028027:CDS
GGTTCAGGACCTTATCCGAA+TGG 0.640623 5.3:+17219882 MS.gene028027:CDS
GATAAGGTCCTGAACCAGGT+GGG 0.657071 5.3:-17219875 None:intergenic
TTGGTTCCAACAACCAACAC+AGG 0.665985 5.3:-17219687 None:intergenic
TGTTGAGAATGTTCGTCCTG+TGG 0.681853 5.3:+17219758 MS.gene028027:CDS
GGATAAGGTCCTGAACCAGG+TGG 0.699303 5.3:-17219876 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAACAATATTAAGATTACT+TGG - chr5.3:17220146-17220165 None:intergenic 15.0%
!!! AATTCTTATAGTTGTTTTTT+TGG + chr5.3:17220336-17220355 MS.gene028027:intron 15.0%
!!! TTCTTTTTCAATTCTATTTT+AGG + chr5.3:17220685-17220704 MS.gene028027:intron 15.0%
!! AAAAAAAGATATAACTTCCA+AGG - chr5.3:17220667-17220686 None:intergenic 20.0%
!! AAAGAAATGAAACTATATCT+TGG - chr5.3:17221094-17221113 None:intergenic 20.0%
!! AAGAATTATGAAGATTTAAC+TGG + chr5.3:17220255-17220274 MS.gene028027:intron 20.0%
!! ATATTGTTAACTCACATAAT+TGG + chr5.3:17220156-17220175 MS.gene028027:intron 20.0%
!! ATTATGATATCTTCTCATTA+TGG + chr5.3:17220041-17220060 MS.gene028027:intron 20.0%
!! TCCATCAAAATGATTTAAAT+GGG + chr5.3:17220373-17220392 MS.gene028027:CDS 20.0%
!!! AGATATTTCAAAAGATTTTG+TGG + chr5.3:17221224-17221243 MS.gene028027:CDS 20.0%
!!! CATGCATAATATTTTTTGAA+TGG - chr5.3:17220298-17220317 None:intergenic 20.0%
!!! CTTTTGTTAACTTACATAAT+TGG + chr5.3:17220096-17220115 MS.gene028027:intron 20.0%
!!! TTCTATAATTTGTGATGTAT+AGG + chr5.3:17221163-17221182 MS.gene028027:intron 20.0%
! AGATGCAAATTAATCTTTCT+AGG + chr5.3:17221004-17221023 MS.gene028027:intron 25.0%
! CTCCATCAAAATGATTTAAA+TGG + chr5.3:17220372-17220391 MS.gene028027:CDS 25.0%
!! ATTAGAGTTTAGAATTGTGA+CGG - chr5.3:17220484-17220503 None:intergenic 25.0%
!! TATTGTAAATACGTATCGAT+TGG - chr5.3:17220888-17220907 None:intergenic 25.0%
!!! CATTTAAATCATTTTGATGG+AGG - chr5.3:17220374-17220393 None:intergenic 25.0%
!!! TGTAAGTTAACAAAAGTACT+TGG - chr5.3:17220092-17220111 None:intergenic 25.0%
!!! TTGGTGGATAAAGTATTTTT+GGG - chr5.3:17221283-17221302 None:intergenic 25.0%
!!! TTTGGTGGATAAAGTATTTT+TGG - chr5.3:17221284-17221303 None:intergenic 25.0%
ACATTCTCAACATGATTCTT+AGG - chr5.3:17219750-17219769 None:intergenic 30.0%
TTGGTATGTATCCAAAACAA+TGG - chr5.3:17221075-17221094 None:intergenic 30.0%
! GCCCATTTAAATCATTTTGA+TGG - chr5.3:17220377-17220396 None:intergenic 30.0%
! TCAACATGATTCTTAGGTTT+TGG - chr5.3:17219744-17219763 None:intergenic 30.0%
! TCTCATTATGGTTCTGTAAT+TGG + chr5.3:17220053-17220072 MS.gene028027:intron 30.0%
! TCTCTGTATCTTTCACATTT+TGG - chr5.3:17221302-17221321 None:intergenic 30.0%
! TGATGTGTCATTCTTGTTTA+AGG - chr5.3:17221358-17221377 None:intergenic 30.0%
! TTCTCAAGTTTGTTCATATC+AGG - chr5.3:17221480-17221499 None:intergenic 30.0%
!! AGGCATAGTTGATTTGATTA+AGG + chr5.3:17220195-17220214 MS.gene028027:intron 30.0%
!! CTGTAATTGGTTTGAGTAAT+TGG + chr5.3:17220066-17220085 MS.gene028027:intron 30.0%
ATGTGAAAGATACAGAGAAG+AGG + chr5.3:17221304-17221323 MS.gene028027:CDS 35.0%
GTAAGTCTTCAATCTATCCT+TGG + chr5.3:17220647-17220666 MS.gene028027:intron 35.0%
TTGCTTTGATACCATTGAAG+TGG + chr5.3:17219972-17219991 MS.gene028027:intron 35.0%
! CTGTATCTTTCACATTTTGG+TGG - chr5.3:17221299-17221318 None:intergenic 35.0%
! GATACTCAACTTTGAAGAAG+TGG + chr5.3:17220816-17220835 MS.gene028027:intron 35.0%
! TGCAAGTGTTTCCATTGTTT+TGG + chr5.3:17221061-17221080 MS.gene028027:intron 35.0%
! TGTGATGTATAGGTTGTTTC+TGG + chr5.3:17221173-17221192 MS.gene028027:intron 35.0%
! TTGGCTAGTTGATCTGTTTA+AGG + chr5.3:17220175-17220194 MS.gene028027:intron 35.0%
!! ATGTATAGGTTGTTTCTGGA+GGG + chr5.3:17221177-17221196 MS.gene028027:intron 35.0%
!!! CATCGTTTTTTGAGTAAACC+GGG + chr5.3:17220218-17220237 MS.gene028027:intron 35.0%
!!! GCATCGTTTTTTGAGTAAAC+CGG + chr5.3:17220217-17220236 MS.gene028027:intron 35.0%
!!! GGATAAAGTATTTTTGGGCA+TGG - chr5.3:17221278-17221297 None:intergenic 35.0%
AACTTAGTTCAAGCTGAGTC+AGG + chr5.3:17220510-17220529 MS.gene028027:CDS 40.0%
AAGTCTACATGGAAGAAACC+TGG + chr5.3:17219639-17219658 MS.gene028027:CDS 40.0%
ATGACCTGACTCACTGATAT+CGG - chr5.3:17220460-17220479 None:intergenic 40.0%
GAACAACAACAGGAGAAAAC+TGG + chr5.3:17220432-17220451 MS.gene028027:CDS 40.0%
GAAGTCATGAAGCAAAGAGA+GGG + chr5.3:17220586-17220605 MS.gene028027:CDS 40.0%
GAATCCTAAGCCTGTATCAT+CGG + chr5.3:17219908-17219927 MS.gene028027:CDS 40.0%
GCAACTAAAAGCCACTTCAA+TGG - chr5.3:17219986-17220005 None:intergenic 40.0%
GGATTCTCATGTTGTTCCAT+AGG - chr5.3:17221390-17221409 None:intergenic 40.0%
GGTTTCTTCCATGTAGACTT+AGG - chr5.3:17219639-17219658 None:intergenic 40.0%
TAGCTCATCTGATTAAGGCT+TGG + chr5.3:17220120-17220139 MS.gene028027:intron 40.0%
TTAGTGCTCCTAAGTCTACA+TGG + chr5.3:17219628-17219647 MS.gene028027:CDS 40.0%
TTTGCTTCATGACTTCCTTC+TGG - chr5.3:17220582-17220601 None:intergenic 40.0%
! ACACTTTTCTCATCAACACC+AGG - chr5.3:17219660-17219679 None:intergenic 40.0%
! TCTTGTTTAAGGTTGTGTCG+TGG - chr5.3:17221347-17221366 None:intergenic 40.0%
! TTGGCTAGCTCATCTGATTA+AGG + chr5.3:17220115-17220134 MS.gene028027:intron 40.0%
!! AAGTGTTGATGTTCCTGTGT+TGG + chr5.3:17219674-17219693 MS.gene028027:CDS 40.0%
!! GATGTATAGGTTGTTTCTGG+AGG + chr5.3:17221176-17221195 MS.gene028027:intron 40.0%
!! TGTATAGGTTGTTTCTGGAG+GGG + chr5.3:17221178-17221197 MS.gene028027:intron 40.0%
!!! TTTTTTGAGTAAACCGGGTG+AGG + chr5.3:17220223-17220242 MS.gene028027:intron 40.0%
CAACTGCATTTGTCTTGAGG+TGG - chr5.3:17221427-17221446 None:intergenic 45.0%
CAAGGACGTTGATCTACATG+CGG - chr5.3:17220859-17220878 None:intergenic 45.0%
CACCAACTGCATTTGTCTTG+AGG - chr5.3:17221430-17221449 None:intergenic 45.0%
CACCTCAAGACAAATGCAGT+TGG + chr5.3:17221425-17221444 MS.gene028027:CDS 45.0%
GCATAGCATGGAACAACAAC+AGG + chr5.3:17220422-17220441 MS.gene028027:CDS 45.0%
GCATGGCTAGCAATTTGAGT+TGG - chr5.3:17221261-17221280 None:intergenic 45.0%
GGAAGTCATGAAGCAAAGAG+AGG + chr5.3:17220585-17220604 MS.gene028027:CDS 45.0%
GGATTCTCACCATTCGGATA+AGG - chr5.3:17219894-17219913 None:intergenic 45.0%
GGCTTAGGATTCTCACCATT+CGG - chr5.3:17219900-17219919 None:intergenic 45.0%
GTTTGAGCATCAGAATGAGC+TGG - chr5.3:17219720-17219739 None:intergenic 45.0%
TGGTTGTTGGAACCAAGTCT+TGG + chr5.3:17219694-17219713 MS.gene028027:CDS 45.0%
TGTTCCATGCTATGCTCAGA+GGG - chr5.3:17220417-17220436 None:intergenic 45.0%
TGTTGAGAATGTTCGTCCTG+TGG + chr5.3:17219758-17219777 MS.gene028027:CDS 45.0%
TTGGTTCCAACAACCAACAC+AGG - chr5.3:17219690-17219709 None:intergenic 45.0%
TTGTTCCATGCTATGCTCAG+AGG - chr5.3:17220418-17220437 None:intergenic 45.0%
! AAAGAGAGGGCGTAAGGAAA+GGG + chr5.3:17220599-17220618 MS.gene028027:CDS 45.0%
! GAAAGGGTACATTGGACAGA+AGG + chr5.3:17220615-17220634 MS.gene028027:CDS 45.0%
! GCGCAACTTGTTCAACTTGT+TGG + chr5.3:17221503-17221522 MS.gene028027:CDS 45.0%
! TCAGGAAGTGTAGAACCGAT+TGG - chr5.3:17221462-17221481 None:intergenic 45.0%
!! CGTATCGATTGGTAGCTACA+AGG - chr5.3:17220877-17220896 None:intergenic 45.0%
!! GATGTTCCTGTGTTGGTTGT+TGG + chr5.3:17219681-17219700 MS.gene028027:CDS 45.0%
ACGAAGACGAACATTGCGCT+TGG - chr5.3:17219811-17219830 None:intergenic 50.0%
AGAATGAGCTGGCCAAGACT+TGG - chr5.3:17219709-17219728 None:intergenic 50.0%
AGATCCCTCTGAGCATAGCA+TGG + chr5.3:17220410-17220429 MS.gene028027:CDS 50.0%
AGGGGCTGGAAAAACATGGT+AGG + chr5.3:17221196-17221215 MS.gene028027:CDS 50.0%
CAAGCTGAGTCAGTACCAGA+AGG + chr5.3:17220564-17220583 MS.gene028027:CDS 50.0%
CAATGTTCGTCTTCGTCCTG+TGG + chr5.3:17219815-17219834 MS.gene028027:CDS 50.0%
CACATCAGTCACTGCTCCTA+TGG + chr5.3:17221371-17221390 MS.gene028027:CDS 50.0%
CGAAGACGAACATTGCGCTT+GGG - chr5.3:17219810-17219829 None:intergenic 50.0%
GAAGACGAACATTGCGCTTG+GGG - chr5.3:17219809-17219828 None:intergenic 50.0%
GATAAGGTCCTGAACCAGGT+GGG - chr5.3:17219878-17219897 None:intergenic 50.0%
GCAGGAACAGCAATGTGAGT+TGG - chr5.3:17219846-17219865 None:intergenic 50.0%
GGCTGGAAAAACATGGTAGG+GGG + chr5.3:17221199-17221218 MS.gene028027:CDS 50.0%
GGGCTGGAAAAACATGGTAG+GGG + chr5.3:17221198-17221217 MS.gene028027:CDS 50.0%
GGGGCTGGAAAAACATGGTA+GGG + chr5.3:17221197-17221216 MS.gene028027:CDS 50.0%
GGTTCAGGACCTTATCCGAA+TGG + chr5.3:17219882-17219901 MS.gene028027:CDS 50.0%
TATGCTTTCCCACCTGGTTC+AGG + chr5.3:17219867-17219886 MS.gene028027:CDS 50.0%
TCACCACGCTATCAAAAGCC+AGG + chr5.3:17219783-17219802 MS.gene028027:CDS 50.0%
TCCTAAGCCTGTATCATCGG+TGG + chr5.3:17219911-17219930 MS.gene028027:CDS 50.0%
TGCTATGCTCAGAGGGATCT+TGG - chr5.3:17220410-17220429 None:intergenic 50.0%
TTCGGATAAGGTCCTGAACC+AGG - chr5.3:17219882-17219901 None:intergenic 50.0%
! CAAAGAGAGGGCGTAAGGAA+AGG + chr5.3:17220598-17220617 MS.gene028027:CDS 50.0%
! GGAACAGCAATGTGAGTTGG+AGG - chr5.3:17219843-17219862 None:intergenic 50.0%
! GGCGTAAGGAAAGGGTACAT+TGG + chr5.3:17220607-17220626 MS.gene028027:CDS 50.0%
! TGAAGCAAAGAGAGGGCGTA+AGG + chr5.3:17220593-17220612 MS.gene028027:CDS 50.0%
!! TTGGTGCTGCTCTGTCCAAT+CGG + chr5.3:17221444-17221463 MS.gene028027:CDS 50.0%
CAAGCTGAGTCAGGAGCAGA+AGG + chr5.3:17220519-17220538 MS.gene028027:CDS 55.0%
CCTACTGCCACCGATGATAC+AGG - chr5.3:17219921-17219940 None:intergenic 55.0%
CCTGCATATGCTTTCCCACC+TGG + chr5.3:17219861-17219880 MS.gene028027:CDS 55.0%
CCTGTATCATCGGTGGCAGT+AGG + chr5.3:17219918-17219937 MS.gene028027:CDS 55.0%
CTGGAGGGGCTGGAAAAACA+TGG + chr5.3:17221192-17221211 MS.gene028027:CDS 55.0%
CTTGAGGTGGCTGTGTCTTG+AGG - chr5.3:17221414-17221433 None:intergenic 55.0%
GAGGGATCTTGGACTGAAGC+TGG - chr5.3:17220399-17220418 None:intergenic 55.0%
GCCACCGATGATACAGGCTT+AGG - chr5.3:17219915-17219934 None:intergenic 55.0%
GGATAAGGTCCTGAACCAGG+TGG - chr5.3:17219879-17219898 None:intergenic 55.0%
TCATCGGTGGCAGTAGGACA+AGG + chr5.3:17219924-17219943 MS.gene028027:CDS 55.0%
TGAGTAAACCGGGTGAGGCT+GGG + chr5.3:17220228-17220247 MS.gene028027:intron 55.0%
TTGAGTAAACCGGGTGAGGC+TGG + chr5.3:17220227-17220246 MS.gene028027:intron 55.0%
! CCAGGTGGGAAAGCATATGC+AGG - chr5.3:17219864-17219883 None:intergenic 55.0%
! TGATAGCGTGGTGATGCCAC+AGG - chr5.3:17219777-17219796 None:intergenic 55.0%
!! ATGTGAGTTGGAGGTGCCAC+AGG - chr5.3:17219834-17219853 None:intergenic 55.0%
!! GGCACCGATATCAGTGAGTC+AGG + chr5.3:17220453-17220472 MS.gene028027:CDS 55.0%
!! TAGGTTGTTTCTGGAGGGGC+TGG + chr5.3:17221182-17221201 MS.gene028027:intron 55.0%
GAGGTGGCTGTGTCTTGAGG+AGG - chr5.3:17221411-17221430 None:intergenic 60.0%
GAGTAAACCGGGTGAGGCTG+GGG + chr5.3:17220229-17220248 MS.gene028027:intron 60.0%
TCTTAGTCCCCAGCCTCACC+CGG - chr5.3:17220239-17220258 None:intergenic 60.0%
!!! GGGCCTGGCTTTTGATAGCG+TGG - chr5.3:17219789-17219808 None:intergenic 60.0%
CGAACATTGCGCTTGGGGCC+TGG - chr5.3:17219804-17219823 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 17219606 17221539 17219606 ID=MS.gene028027
chr5.3 mRNA 17219606 17221539 17219606 ID=MS.gene028027.t1;Parent=MS.gene028027
chr5.3 exon 17219606 17219945 17219606 ID=MS.gene028027.t1.exon1;Parent=MS.gene028027.t1
chr5.3 CDS 17219606 17219945 17219606 ID=cds.MS.gene028027.t1;Parent=MS.gene028027.t1
chr5.3 exon 17220370 17220646 17220370 ID=MS.gene028027.t1.exon2;Parent=MS.gene028027.t1
chr5.3 CDS 17220370 17220646 17220370 ID=cds.MS.gene028027.t1;Parent=MS.gene028027.t1
chr5.3 exon 17221185 17221539 17221185 ID=MS.gene028027.t1.exon3;Parent=MS.gene028027.t1
chr5.3 CDS 17221185 17221539 17221185 ID=cds.MS.gene028027.t1;Parent=MS.gene028027.t1
Gene Sequence

>MS.gene028027

ATGGTGATTGCTGATAATGAGATTAGTGCTCCTAAGTCTACATGGAAGAAACCTGGTGTTGATGAGAAAAGTGTTGATGTTCCTGTGTTGGTTGTTGGAACCAAGTCTTGGCCAGCTCATTCTGATGCTCAAACTCCAAAACCTAAGAATCATGTTGAGAATGTTCGTCCTGTGGCATCACCACGCTATCAAAAGCCAGGCCCCAAGCGCAATGTTCGTCTTCGTCCTGTGGCACCTCCAACTCACATTGCTGTTCCTGCATATGCTTTCCCACCTGGTTCAGGACCTTATCCGAATGGTGAGAATCCTAAGCCTGTATCATCGGTGGCAGTAGGACAAGGTGAGAATCACTAATCTTCAATATTCTTGCTTTGATACCATTGAAGTGGCTTTTAGTTGCAACATGATGTTTTACAATGAGTGTTTTTTATTGTCATTATGATATCTTCTCATTATGGTTCTGTAATTGGTTTGAGTAATTGGCCAAGTACTTTTGTTAACTTACATAATTGGCTAGCTCATCTGATTAAGGCTTGGCCAAGTAATCTTAATATTGTTAACTCACATAATTGGCTAGTTGATCTGTTTAAGGCATAGTTGATTTGATTAAGGCATCGTTTTTTGAGTAAACCGGGTGAGGCTGGGGACTAAGAATTATGAAGATTTAACTGGTATCTAACATATTTTTACCATTCAAAAAATATTATGCATGTCGATTTAACTAGTTTTAAATTCTTATAGTTGTTTTTTTGGTATACAATTAGACCTCCATCAAAATGATTTAAATGGGCCAGCTTCAGTCCAAGATCCCTCTGAGCATAGCATGGAACAACAACAGGAGAAAACTGGCACCGATATCAGTGAGTCAGGTCATGCCGTCACAATTCTAAACTCTAATGATAATAACTTAGTTCAAGCTGAGTCAGGAGCAGAAGGAAGTAATGAAGCTAACTTAGTTCAAGCTGAGTCAGTACCAGAAGGAAGTCATGAAGCAAAGAGAGGGCGTAAGGAAAGGGTACATTGGACAGAAGGTGAACACAAGTAAGTCTTCAATCTATCCTTGGAAGTTATATCTTTTTTTCTTTTTCAATTCTATTTTAGGTTAAATATATTCTTTTCAATTTAAGTTATTAGATGAAGTATTAAATAGTTGTTAGATCGCATAACTACAAATTTATATTTTTATTTTAGACGAATAGTCTAGTGACTAGATACTCAACTTTGAAGAAGTGGAATATTTTCGATTCGAACCGCATGTAGATCAACGTCCTTGTAGCTACCAATCGATACGTATTTACAATACATTAATAAAAAAACACATTTATAGCTTTTTAGTTTGTATGATGTATATAAGAAATAATTGAGTTCTAGAAAAACAAAAATGATATTTATGTTCATAGATGCAAATTAATCTTTCTAGGTTGCAAATCAACAAAGAGAAGAGAAAAAAATCAATGCAAGTGTTTCCATTGTTTTGGATACATACCAAGATATAGTTTCATTTCTTTATTATTTTCTTCTCTTATTATTTAATTGAATTTGAATATTTCGCATTCATTCTATAATTTGTGATGTATAGGTTGTTTCTGGAGGGGCTGGAAAAACATGGTAGGGGGAAAGATATTTCAAAAGATTTTGTGGTGACAAAGACTCCAACTCAAATTGCTAGCCATGCCCAAAAATACTTTATCCACCAAAATGTGAAAGATACAGAGAAGAGGAAAAAGAGAAGAAGTATCCACGACACAACCTTAAACAAGAATGACACATCAGTCACTGCTCCTATGGAACAACATGAGAATCCTCCTCAAGACACAGCCACCTCAAGACAAATGCAGTTGGTGCTGCTCTGTCCAATCGGTTCTACACTTCCTGATATGAACAAACTTGAGAAGATGCGCAACTTGTTCAACTTGTTGGCAAAAGAACTTTGA
Protein sequence

>MS.gene028027.t1

MVIADNEISAPKSTWKKPGVDEKSVDVPVLVVGTKSWPAHSDAQTPKPKNHVENVRPVASPRYQKPGPKRNVRLRPVAPPTHIAVPAYAFPPGSGPYPNGENPKPVSSVAVGQDLHQNDLNGPASVQDPSEHSMEQQQEKTGTDISESGHAVTILNSNDNNLVQAESGAEGSNEANLVQAESVPEGSHEAKRGRKERVHWTEGEHKLFLEGLEKHGRGKDISKDFVVTKTPTQIASHAQKYFIHQNVKDTEKRKKRRSIHDTTLNKNDTSVTAPMEQHENPPQDTATSRQMQLVLLCPIGSTLPDMNKLEKMRNLFNLLAKEL