AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028039

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028039.t1 MTR_5g027440 93.885 278 16 1 1 277 1 278 0.0 538
MS.gene028039.t1 MTR_6g444980 52.147 163 65 3 10 171 22 172 6.44e-52 174
MS.gene028039.t1 MTR_2g086450 50.000 194 71 4 18 193 38 223 1.76e-50 171
MS.gene028039.t1 MTR_6g032990 49.000 200 78 5 18 204 35 223 1.93e-49 168
MS.gene028039.t1 MTR_6g444980 48.864 176 77 3 10 184 22 185 1.08e-48 165
MS.gene028039.t1 MTR_7g088070 54.545 143 52 1 25 167 50 179 2.83e-48 162
MS.gene028039.t1 MTR_7g088070 57.241 145 49 3 25 167 50 183 2.37e-47 159
MS.gene028039.t1 MTR_4g081710 50.920 163 67 4 15 171 7 162 1.72e-45 156
MS.gene028039.t1 MTR_5g041350 50.000 162 71 4 15 171 5 161 2.73e-44 153
MS.gene028039.t1 MTR_2g027860 39.526 253 127 5 25 270 49 282 3.08e-44 153
MS.gene028039.t1 MTR_2g027800 47.977 173 74 4 7 171 20 184 3.66e-43 149
MS.gene028039.t1 MTR_1g090670 51.282 156 68 4 18 171 37 186 2.70e-42 147
MS.gene028039.t1 MTR_2g027800 48.193 166 70 4 7 171 20 170 1.31e-40 142
MS.gene028039.t1 MTR_7g115530 46.012 163 76 3 25 187 218 368 4.76e-36 134
MS.gene028039.t1 MTR_7g098250 49.645 141 59 2 25 165 262 390 6.82e-36 134
MS.gene028039.t1 MTR_1g053835 44.755 143 65 2 25 167 258 386 4.86e-34 128
MS.gene028039.t1 MTR_1g080330 46.622 148 67 3 18 165 168 303 4.90e-34 127
MS.gene028039.t1 MTR_7g093030 40.816 147 74 3 25 171 212 345 3.30e-30 117
MS.gene028039.t1 MTR_1g080330 47.244 127 55 3 18 144 168 282 2.28e-27 107
MS.gene028039.t1 MTR_7g115530 48.333 120 50 3 25 144 218 325 3.60e-26 105
MS.gene028039.t1 MTR_7g068600 39.860 143 67 3 25 162 13 141 7.27e-25 99.8
MS.gene028039.t1 MTR_7g115530 58.462 65 27 0 25 89 218 282 4.51e-21 90.9
MS.gene028039.t1 MTR_7g115530 52.000 75 34 1 25 97 218 292 4.67e-20 89.0
MS.gene028039.t1 MTR_7g093030 57.895 57 24 0 25 81 212 268 8.66e-20 87.0
MS.gene028039.t1 MTR_1g053800 33.714 175 92 4 12 178 159 317 2.63e-19 86.3
MS.gene028039.t1 MTR_1g053830 55.357 56 25 0 27 82 20 75 6.83e-19 80.1
MS.gene028039.t1 MTR_2g023580 48.387 93 46 1 21 113 51 141 9.32e-19 83.2
MS.gene028039.t1 MTR_1g112370 47.059 102 42 3 1 97 1 95 1.93e-18 84.0
MS.gene028039.t1 MTR_7g069660 62.069 58 22 0 21 78 67 124 2.46e-18 84.0
MS.gene028039.t1 MTR_7g115530 65.385 52 18 0 25 76 218 269 5.30e-18 82.0
MS.gene028039.t1 MTR_0223s0040 56.716 67 29 0 12 78 5 71 1.08e-17 81.3
MS.gene028039.t1 MTR_6g071625 61.667 60 23 0 26 85 203 262 1.66e-17 81.6
MS.gene028039.t1 MTR_8g066310 69.811 53 16 0 26 78 174 226 2.31e-17 81.3
MS.gene028039.t1 MTR_7g115530 62.264 53 20 0 25 77 218 270 2.89e-17 80.1
MS.gene028039.t1 MTR_5g029470 69.811 53 16 0 26 78 220 272 7.54e-17 80.1
MS.gene028039.t1 MTR_1g021520 67.925 53 17 0 26 78 127 179 8.03e-17 79.0
MS.gene028039.t1 MTR_2g084230 60.345 58 23 0 21 78 93 150 1.15e-16 78.6
MS.gene028039.t1 MTR_8g086410 51.389 72 28 1 7 78 100 164 1.21e-16 79.7
MS.gene028039.t1 MTR_8g086410 51.389 72 28 1 7 78 98 162 1.29e-16 79.3
MS.gene028039.t1 MTR_2g084230 60.345 58 23 0 21 78 93 150 1.42e-16 79.0
MS.gene028039.t1 MTR_3g450310 48.611 72 30 1 7 78 8 72 2.04e-16 76.3
MS.gene028039.t1 MTR_3g064840 67.925 53 17 0 26 78 275 327 2.14e-16 79.0
MS.gene028039.t1 MTR_7g093010 36.420 162 78 6 15 168 200 344 2.27e-16 78.6
MS.gene028039.t1 MTR_2g016220 49.333 75 38 0 4 78 68 142 1.01e-15 76.6
MS.gene028039.t1 MTR_2g016220 49.333 75 38 0 4 78 68 142 1.05e-15 76.6
MS.gene028039.t1 MTR_1g090670 42.735 117 59 4 57 171 1 111 1.15e-15 74.3
MS.gene028039.t1 MTR_8g086410 48.611 72 30 1 7 78 100 164 2.11e-15 75.9
MS.gene028039.t1 MTR_2g016220 48.611 72 37 0 4 75 68 139 3.12e-14 72.4
MS.gene028039.t1 MTR_4g121020 58.182 55 22 1 25 79 111 164 5.61e-13 68.9
MS.gene028039.t1 MTR_4g121020 58.182 55 22 1 25 79 215 268 6.78e-13 68.9
MS.gene028039.t1 MTR_4g121020 58.182 55 22 1 25 79 215 268 6.90e-13 68.9
MS.gene028039.t1 MTR_5g017980 47.222 72 36 1 25 96 193 262 4.45e-12 65.9
MS.gene028039.t1 MTR_7g089010 56.364 55 23 1 25 79 91 144 5.60e-12 65.1
MS.gene028039.t1 MTR_4g113140 40.196 102 48 2 25 115 235 334 7.09e-12 65.5
MS.gene028039.t1 MTR_2g450070 52.727 55 25 1 25 79 213 266 7.16e-12 65.9
MS.gene028039.t1 MTR_2g450070 52.727 55 25 1 25 79 213 266 7.31e-12 65.9
MS.gene028039.t1 MTR_1g093080 50.820 61 28 1 25 85 211 269 1.04e-11 64.7
MS.gene028039.t1 MTR_4g064730 54.545 55 24 1 25 79 131 184 1.22e-11 64.3
MS.gene028039.t1 MTR_1g032570 52.727 55 25 1 25 79 131 184 1.35e-11 64.7
MS.gene028039.t1 MTR_1g032570 52.727 55 25 1 25 79 200 253 1.68e-11 64.7
MS.gene028039.t1 MTR_4g086835 54.098 61 26 1 25 85 274 332 1.94e-11 64.3
MS.gene028039.t1 MTR_4g021790 46.154 65 32 2 25 89 168 229 2.75e-11 62.8
MS.gene028039.t1 MTR_1g013170 54.237 59 26 1 21 79 187 244 2.93e-11 63.9
MS.gene028039.t1 MTR_5g041350 60.417 48 19 0 124 171 3 50 7.13e-11 61.2
MS.gene028039.t1 MTR_5g017980 47.222 72 35 2 25 96 192 260 9.69e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028039.t1 AT5G45580 49.655 290 118 11 2 274 1 279 4.04e-76 233
MS.gene028039.t1 AT5G45580 50.368 272 108 10 20 274 2 263 7.19e-74 227
MS.gene028039.t1 AT3G24120 53.333 180 80 3 18 194 34 212 6.00e-58 187
MS.gene028039.t1 AT4G13640 50.575 174 84 1 17 188 29 202 3.30e-57 185
MS.gene028039.t1 AT3G24120 52.459 183 80 4 18 194 34 215 8.70e-56 182
MS.gene028039.t1 AT4G13640 49.718 177 84 2 17 188 29 205 4.68e-55 180
MS.gene028039.t1 AT5G18240 48.718 195 87 4 7 193 29 218 1.68e-51 174
MS.gene028039.t1 AT5G18240 48.718 195 87 4 7 193 29 218 1.68e-51 174
MS.gene028039.t1 AT3G04030 47.739 199 83 4 7 193 29 218 1.25e-50 171
MS.gene028039.t1 AT1G79430 49.693 163 69 3 10 171 21 171 3.66e-48 164
MS.gene028039.t1 AT5G18240 47.692 195 87 5 7 193 29 216 4.62e-48 164
MS.gene028039.t1 AT3G12730 47.273 165 69 4 18 173 16 171 5.38e-48 160
MS.gene028039.t1 AT3G04030 46.734 199 84 5 7 193 29 217 1.57e-47 163
MS.gene028039.t1 AT5G18240 46.667 195 85 5 7 193 29 212 1.95e-46 160
MS.gene028039.t1 AT5G18240 46.667 195 85 5 7 193 29 212 1.95e-46 160
MS.gene028039.t1 AT3G04030 45.729 199 81 5 7 193 29 212 9.43e-46 158
MS.gene028039.t1 AT1G69580 48.810 168 81 2 16 178 25 192 2.95e-45 155
MS.gene028039.t1 AT1G69580 47.929 169 82 2 16 178 25 193 1.41e-44 154
MS.gene028039.t1 AT2G01060 54.545 143 54 2 25 167 15 146 1.52e-43 150
MS.gene028039.t1 AT3G13040 46.250 160 76 2 25 181 241 393 9.89e-38 138
MS.gene028039.t1 AT3G13040 46.250 160 76 2 25 181 241 393 9.89e-38 138
MS.gene028039.t1 AT3G13040 46.250 160 76 2 25 181 241 393 9.89e-38 138
MS.gene028039.t1 AT4G28610 45.000 160 76 3 25 184 225 372 1.27e-34 129
MS.gene028039.t1 AT5G06800 44.755 143 71 3 25 167 192 326 1.06e-32 124
MS.gene028039.t1 AT5G29000 44.828 145 67 2 25 167 188 321 2.64e-32 122
MS.gene028039.t1 AT5G29000 44.828 145 67 2 25 167 188 321 2.64e-32 122
MS.gene028039.t1 AT3G04450 45.205 146 65 2 25 167 198 331 3.11e-32 122
MS.gene028039.t1 AT3G04450 45.205 146 65 2 25 167 198 331 3.11e-32 122
MS.gene028039.t1 AT3G04450 45.205 146 65 2 25 167 238 371 4.10e-32 123
MS.gene028039.t1 AT5G06800 44.755 143 66 3 25 167 192 321 4.39e-32 122
MS.gene028039.t1 AT5G29000 44.828 145 67 2 25 167 231 364 4.61e-32 122
MS.gene028039.t1 AT5G45580 39.269 219 106 10 73 274 16 224 5.87e-31 115
MS.gene028039.t1 AT3G04030 45.638 149 62 3 57 193 1 142 8.83e-28 109
MS.gene028039.t1 AT2G20400 43.885 139 67 2 27 165 233 360 3.16e-27 109
MS.gene028039.t1 AT2G20400 43.165 139 67 2 27 165 233 359 1.16e-26 107
MS.gene028039.t1 AT5G18240 43.448 145 65 4 57 193 1 136 1.43e-24 100
MS.gene028039.t1 AT2G06020 42.424 132 50 4 3 113 58 184 1.06e-23 98.2
MS.gene028039.t1 AT3G04030 42.953 149 60 4 57 193 1 136 4.85e-23 96.7
MS.gene028039.t1 AT1G79430 43.966 116 54 2 57 171 1 106 4.33e-22 93.6
MS.gene028039.t1 AT2G01060 48.148 108 45 2 60 167 1 97 6.30e-22 92.0
MS.gene028039.t1 AT3G12730 38.889 126 59 4 57 173 1 117 2.19e-21 89.0
MS.gene028039.t1 AT5G06800 53.731 67 31 0 25 91 192 258 5.31e-20 87.4
MS.gene028039.t1 AT2G42660 69.811 53 16 0 26 78 51 103 2.96e-19 85.1
MS.gene028039.t1 AT2G02060 51.765 85 37 1 1 81 1 85 6.37e-19 84.3
MS.gene028039.t1 AT2G02060 51.765 85 37 1 1 81 1 85 6.37e-19 84.3
MS.gene028039.t1 AT4G04605 55.556 63 28 0 16 78 5 67 8.52e-19 83.2
MS.gene028039.t1 AT5G29000 53.623 69 32 0 25 93 231 299 2.00e-18 83.6
MS.gene028039.t1 AT1G14600 56.000 75 31 1 6 78 2 76 2.20e-18 82.8
MS.gene028039.t1 AT3G04450 56.897 58 25 0 25 82 238 295 4.26e-18 82.8
MS.gene028039.t1 AT2G40260 57.812 64 27 0 15 78 72 135 4.86e-18 83.6
MS.gene028039.t1 AT4G04580 47.500 80 37 1 14 93 3 77 8.63e-18 79.0
MS.gene028039.t1 AT1G32240 69.811 53 16 0 26 78 214 266 4.51e-17 80.5
MS.gene028039.t1 AT1G32240 69.811 53 16 0 26 78 214 266 5.79e-17 80.5
MS.gene028039.t1 AT5G16560 67.925 53 17 0 26 78 220 272 7.86e-17 80.1
MS.gene028039.t1 AT5G42630 66.038 53 18 0 26 78 106 158 8.51e-17 77.8
MS.gene028039.t1 AT2G38300 61.404 57 22 0 22 78 51 107 1.36e-16 79.0
MS.gene028039.t1 AT5G42630 66.038 53 18 0 26 78 106 158 2.19e-16 77.4
MS.gene028039.t1 AT4G17695 66.038 53 18 0 26 78 165 217 3.55e-16 77.4
MS.gene028039.t1 AT3G25790 39.286 112 51 4 30 128 201 308 2.07e-13 69.7
MS.gene028039.t1 AT4G37180 55.932 59 24 1 27 85 219 275 2.93e-13 69.3
MS.gene028039.t1 AT4G37180 55.932 59 24 1 27 85 212 268 3.07e-13 69.3
MS.gene028039.t1 AT1G49560 45.263 95 48 2 25 117 192 284 6.15e-13 68.2
MS.gene028039.t1 AT3G10760 51.351 74 31 3 6 79 90 158 9.08e-13 67.8
MS.gene028039.t1 AT5G05090 56.364 55 23 1 25 79 81 134 2.45e-12 65.9
MS.gene028039.t1 AT4G04555 46.032 63 34 0 16 78 5 67 2.90e-12 62.4
MS.gene028039.t1 AT3G46640 54.545 55 24 1 25 79 144 197 4.89e-12 65.5
MS.gene028039.t1 AT3G46640 54.545 55 24 1 25 79 144 197 4.89e-12 65.5
MS.gene028039.t1 AT5G59570 54.545 55 24 1 25 79 141 194 5.14e-12 65.5
MS.gene028039.t1 AT5G59570 54.545 55 24 1 25 79 141 194 5.14e-12 65.5
MS.gene028039.t1 AT3G46640 54.545 55 24 1 25 79 144 197 5.67e-12 65.5
MS.gene028039.t1 AT5G58080 56.364 55 23 1 25 79 194 247 6.12e-12 65.9
MS.gene028039.t1 AT2G40970 56.364 55 23 1 25 79 105 158 9.26e-12 63.9
MS.gene028039.t1 AT2G03500 54.098 61 26 1 25 85 233 291 2.23e-11 63.9
MS.gene028039.t1 AT1G13300 50.820 61 28 1 25 85 181 239 3.22e-11 63.2
MS.gene028039.t1 AT3G16857 52.727 55 25 1 25 79 167 220 3.53e-11 63.9
MS.gene028039.t1 AT3G16857 52.727 55 25 1 25 79 237 290 3.88e-11 63.5
MS.gene028039.t1 AT3G16857 52.727 55 25 1 25 79 237 290 4.13e-11 63.5
MS.gene028039.t1 AT2G01760 52.727 55 25 1 25 79 135 188 5.97e-11 62.4

Find 70 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGAGCATGATTCTTATTA+TGG 0.152516 5.3:+17028571 None:intergenic
CTCAGCTTTGTCAAATTTCA+TGG 0.192891 5.3:-17028643 MS.gene028039:CDS
TGCCTTAGTGCTTCTCCTAT+TGG 0.203121 5.3:+17029229 None:intergenic
GCCTTAGTGCTTCTCCTATT+GGG 0.237177 5.3:+17029230 None:intergenic
CTAAGGTTAATGGGATTAAA+AGG 0.242641 5.3:-17030147 MS.gene028039:CDS
TAAGTCTGTTCTAAGGTTAA+TGG 0.265341 5.3:-17030157 MS.gene028039:CDS
GTCCACCTCAGCCTCGGCTT+CGG 0.273883 5.3:+17030635 None:intergenic
CAGCTAGACTTAAACATTAA+AGG 0.275731 5.3:-17028474 MS.gene028039:CDS
ATCAAATATGGTTTCATATA+GGG 0.282950 5.3:-17028412 MS.gene028039:CDS
AGAGGCTCAAGGGAAGTATT+TGG 0.284186 5.3:-17028754 MS.gene028039:CDS
TCCACCTCAGCCTCGGCTTC+GGG 0.297458 5.3:+17030636 None:intergenic
TCATTTGAAGAGCCATTTAC+AGG 0.303807 5.3:-17030112 MS.gene028039:intron
AATTTCAGTAACCATTCTTC+AGG 0.305474 5.3:-17029515 MS.gene028039:CDS
AAGTCTGTTCTAAGGTTAAT+GGG 0.346285 5.3:-17030156 MS.gene028039:CDS
AGCTGAAATGGAATCAAATA+TGG 0.349100 5.3:-17028424 MS.gene028039:CDS
AATCAAATATGGTTTCATAT+AGG 0.356297 5.3:-17028413 MS.gene028039:CDS
GGAGGGAGAGGAGGAATTGA+AGG 0.362824 5.3:-17030692 MS.gene028039:CDS
ACTAGGGACAGAAGAAAATA+AGG 0.364063 5.3:-17028529 MS.gene028039:CDS
TAGAAGTTGTTACCTGTAAA+TGG 0.368392 5.3:+17030100 None:intergenic
TAATGCATCAAAAGCTCAAT+TGG 0.371170 5.3:-17028676 MS.gene028039:CDS
AATTGAAGTTCAAAAGAGAT+TGG 0.388648 5.3:-17029203 MS.gene028039:CDS
TCAGGGACTAATACCAACTA+TGG 0.403640 5.3:-17029497 MS.gene028039:CDS
ATCTGTAGATGGAGCTGAAA+TGG 0.439210 5.3:-17028436 MS.gene028039:CDS
TGCTCTGTCCATCTTGAGAA+AGG 0.439571 5.3:+17028703 None:intergenic
ATGATGGAAAGTGGAGGGAG+AGG 0.440510 5.3:-17030704 MS.gene028039:CDS
TTCAATTATCAAGAAGTACT+AGG 0.442529 5.3:-17028546 MS.gene028039:CDS
CGACTCCTAAGTCTGTTCTA+AGG 0.455099 5.3:-17030164 MS.gene028039:CDS
GGAGAGGGTATGATGGAAAG+TGG 0.456884 5.3:-17030713 None:intergenic
GGAGGAATTGAAGGGTATGA+TGG 0.460307 5.3:-17030683 MS.gene028039:CDS
GAACTAAAGCCTCAAGTTGA+AGG 0.464648 5.3:-17028507 MS.gene028039:CDS
GTCGACGCTGTCACAAAGCT+TGG 0.467980 5.3:-17030590 MS.gene028039:CDS
ATTAACCTTAGAACAGACTT+AGG 0.473463 5.3:+17030159 None:intergenic
TAAATTACAAATGAGAATAG+AGG 0.480036 5.3:-17028772 MS.gene028039:CDS
TGGTATTAGTCCCTGAAGAA+TGG 0.487795 5.3:+17029504 None:intergenic
ACCCGAAGCCGAGGCTGAGG+TGG 0.491348 5.3:-17030637 MS.gene028039:CDS
TTCACTATCACCTCCATAGT+TGG 0.497330 5.3:+17029484 None:intergenic
ATTCACAGAGAAATCCCAAT+AGG 0.498124 5.3:-17029244 MS.gene028039:intron
ATTTCAGTAACCATTCTTCA+GGG 0.498736 5.3:-17029514 MS.gene028039:CDS
AATGAACTTGTATCTGTAGA+TGG 0.501826 5.3:-17028447 MS.gene028039:CDS
GAGACCCGAAGCCGAGGCTG+AGG 0.504025 5.3:-17030640 MS.gene028039:CDS
ATGGAAAGTGGAGGGAGAGG+AGG 0.521924 5.3:-17030701 MS.gene028039:CDS
ATGGTTACTGAAATTTACAT+AGG 0.523278 5.3:+17029523 None:intergenic
GATAGTAAAGAAAACATACT+AGG 0.533466 5.3:-17028606 MS.gene028039:CDS
GAGGGAGAGGAGGAATTGAA+GGG 0.537312 5.3:-17030691 MS.gene028039:CDS
AACTAAAGCCTCAAGTTGAA+GGG 0.540564 5.3:-17028506 MS.gene028039:CDS
GACGCTGTCACAAAGCTTGG+TGG 0.544188 5.3:-17030587 MS.gene028039:CDS
TCAATTATCAAGAAGTACTA+GGG 0.553916 5.3:-17028545 MS.gene028039:CDS
ACTAAAGCCTCAAGTTGAAG+GGG 0.554788 5.3:-17028505 MS.gene028039:CDS
AACTTGCAGAAGTATAGACT+TGG 0.555358 5.3:-17029799 MS.gene028039:intron
TCCCAATAGGAGAAGCACTA+AGG 0.559258 5.3:-17029231 MS.gene028039:CDS
CAAACAAGCCTTTCTCAAGA+TGG 0.560229 5.3:-17028711 MS.gene028039:CDS
GAGGGTATGATGGAAAGTGG+AGG 0.565667 5.3:-17030710 None:intergenic
GACAAAGCGGTCATGAAGAT+CGG 0.565953 5.3:+17030610 None:intergenic
AGGGTATGATGGAAAGTGGA+GGG 0.568191 5.3:-17030709 None:intergenic
ACTATGGAGGTGATAGTGAA+GGG 0.577334 5.3:-17029481 MS.gene028039:CDS
AACTATGGAGGTGATAGTGA+AGG 0.581387 5.3:-17029482 MS.gene028039:CDS
GGCTCAAGGGAAGTATTTGG+AGG 0.583375 5.3:-17028751 MS.gene028039:CDS
AAATGAGAATAGAGGCTCAA+GGG 0.584563 5.3:-17028764 MS.gene028039:CDS
TCGGTTGTCCACCTCAGCCT+CGG 0.594438 5.3:+17030629 None:intergenic
TGACGAGAGACCCGAAGCCG+AGG 0.608614 5.3:-17030646 MS.gene028039:CDS
CAAATGAGAATAGAGGCTCA+AGG 0.629250 5.3:-17028765 MS.gene028039:CDS
AGAAGAGGTACATACTGTCA+GGG 0.640171 5.3:+17030564 None:intergenic
TATGGAGGTGATAGTGAAGG+GGG 0.650745 5.3:-17029479 MS.gene028039:CDS
CTATGGAGGTGATAGTGAAG+GGG 0.663409 5.3:-17029480 MS.gene028039:CDS
GGGACTAATACCAACTATGG+AGG 0.669862 5.3:-17029494 MS.gene028039:CDS
CTAGACTTAAACATTAAAGG+TGG 0.670077 5.3:-17028471 MS.gene028039:CDS
GAGATTGGAAGAACAACTTG+AGG 0.683401 5.3:-17029188 MS.gene028039:intron
TTGTGACAGCGTCGACAAAG+CGG 0.689498 5.3:+17030597 None:intergenic
CTAAAGCCTCAAGTTGAAGG+GGG 0.707595 5.3:-17028504 MS.gene028039:CDS
ATGGAGGTGATAGTGAAGGG+GGG 0.714692 5.3:-17029478 MS.gene028039:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACACTAAAATTTTAAATAA+AGG + chr5.3:17028667-17028686 None:intergenic 10.0%
!!! ATGATATATTTAATTTATGA+AGG - chr5.3:17029090-17029109 MS.gene028039:intron 10.0%
!!! ATTTTATTTTTTGACTAAAA+AGG + chr5.3:17029031-17029050 None:intergenic 10.0%
!!! TCTAAATATTTTCTTATTAA+TGG - chr5.3:17028827-17028846 MS.gene028039:intron 10.0%
!! AATTAAAATGCTAATTACAT+AGG + chr5.3:17029792-17029811 None:intergenic 15.0%
!! ACATTTATGTTAACTTTAAA+GGG - chr5.3:17028693-17028712 MS.gene028039:CDS 15.0%
!! TATTATCATTAATTAGAGAA+TGG - chr5.3:17029179-17029198 MS.gene028039:intron 15.0%
!! AAATGTTGAATTAATCTCTA+TGG - chr5.3:17028761-17028780 MS.gene028039:CDS 20.0%
!! AATCAAATATGGTTTCATAT+AGG - chr5.3:17030689-17030708 MS.gene028039:CDS 20.0%
!! ATAAACTAGCAGTTAAAAAT+AGG - chr5.3:17029114-17029133 MS.gene028039:intron 20.0%
!! ATCAAATATGGTTTCATATA+GGG - chr5.3:17030690-17030709 MS.gene028039:CDS 20.0%
!! CACATTTATGTTAACTTTAA+AGG - chr5.3:17028692-17028711 MS.gene028039:CDS 20.0%
!! TAAATTACAAATGAGAATAG+AGG - chr5.3:17030330-17030349 MS.gene028039:intron 20.0%
!! TATATCATCACTATGTAAAA+AGG + chr5.3:17029078-17029097 None:intergenic 20.0%
!! TTTGTTAAAATTGACATTGA+AGG - chr5.3:17028724-17028743 MS.gene028039:CDS 20.0%
!!! GCAATTATTTCTATTACATT+TGG - chr5.3:17029522-17029541 MS.gene028039:CDS 20.0%
!!! TTATTTTTTGACTAAAAAGG+TGG + chr5.3:17029028-17029047 None:intergenic 20.0%
!!! TTTTTGTCTTTCATCTTTTT+AGG - chr5.3:17029551-17029570 MS.gene028039:intron 20.0%
! AATTGAAGTTCAAAAGAGAT+TGG - chr5.3:17029899-17029918 MS.gene028039:intron 25.0%
! ATGGTTACTGAAATTTACAT+AGG + chr5.3:17029582-17029601 None:intergenic 25.0%
! ATGTAAAAAGGCAAAAGAAA+AGG + chr5.3:17029066-17029085 None:intergenic 25.0%
! GAATGATAAGCTTTCAAAAT+TGG + chr5.3:17028809-17028828 None:intergenic 25.0%
! GATAGTAAAGAAAACATACT+AGG - chr5.3:17030496-17030515 MS.gene028039:intron 25.0%
! TAAGGATATATATACTCCTT+TGG + chr5.3:17030257-17030276 None:intergenic 25.0%
! TTCTCAATTGTCAATTACAT+AGG + chr5.3:17030026-17030045 None:intergenic 25.0%
!!! AGAAGTTTTAGATGAGAATT+TGG + chr5.3:17029767-17029786 None:intergenic 25.0%
!!! TCAATTATCAAGAAGTACTA+GGG - chr5.3:17030557-17030576 MS.gene028039:intron 25.0%
!!! TTCAATTATCAAGAAGTACT+AGG - chr5.3:17030556-17030575 MS.gene028039:intron 25.0%
!!! TTTTGAAAGAGATGAGAATT+TGG + chr5.3:17029727-17029746 None:intergenic 25.0%
AAAGAGTCTCAAATAAGTGA+AGG - chr5.3:17029394-17029413 MS.gene028039:intron 30.0%
AAGAGTCTCAAATAAGTGAA+GGG - chr5.3:17029395-17029414 MS.gene028039:intron 30.0%
AATGAACTTGTATCTGTAGA+TGG - chr5.3:17030655-17030674 MS.gene028039:CDS 30.0%
AATTAATCTCTATGGACTTG+TGG - chr5.3:17028769-17028788 MS.gene028039:CDS 30.0%
AATTTCAGTAACCATTCTTC+AGG - chr5.3:17029587-17029606 MS.gene028039:intron 30.0%
ACAAAACTAGATGAAAGAAG+AGG + chr5.3:17028556-17028575 None:intergenic 30.0%
AGCTGAAATGGAATCAAATA+TGG - chr5.3:17030678-17030697 MS.gene028039:CDS 30.0%
ATTAACCTTAGAACAGACTT+AGG + chr5.3:17028946-17028965 None:intergenic 30.0%
ATTTCAGTAACCATTCTTCA+GGG - chr5.3:17029588-17029607 MS.gene028039:intron 30.0%
CAATTGGACTGTTTAATTCT+AGG - chr5.3:17030300-17030319 MS.gene028039:intron 30.0%
CAGCTAGACTTAAACATTAA+AGG - chr5.3:17030628-17030647 MS.gene028039:CDS 30.0%
CTAAGGTTAATGGGATTAAA+AGG - chr5.3:17028955-17028974 MS.gene028039:intron 30.0%
CTAGAATTAAACAGTCCAAT+TGG + chr5.3:17030302-17030321 None:intergenic 30.0%
CTAGACTTAAACATTAAAGG+TGG - chr5.3:17030631-17030650 MS.gene028039:CDS 30.0%
CTAGTGAATTTCTAACCAAT+TGG - chr5.3:17030284-17030303 MS.gene028039:intron 30.0%
CTTGAGCATGATTCTTATTA+TGG + chr5.3:17030534-17030553 None:intergenic 30.0%
GAATTGTCGTTAAACTAGTT+AGG + chr5.3:17029842-17029861 None:intergenic 30.0%
GGTTAGAAATTCACTAGTTA+GGG + chr5.3:17030281-17030300 None:intergenic 30.0%
GTCTTATAATAAGAGACCAA+AGG - chr5.3:17030238-17030257 MS.gene028039:intron 30.0%
TAATGCATCAAAAGCTCAAT+TGG - chr5.3:17030426-17030445 MS.gene028039:intron 30.0%
TAGAAGTTGTTACCTGTAAA+TGG + chr5.3:17029005-17029024 None:intergenic 30.0%
TGGTTAGAAATTCACTAGTT+AGG + chr5.3:17030282-17030301 None:intergenic 30.0%
! GACTCACATACTAATTTTAG+TGG + chr5.3:17030221-17030240 None:intergenic 30.0%
!! AAGTCTGTTCTAAGGTTAAT+GGG - chr5.3:17028946-17028965 MS.gene028039:intron 30.0%
!! TAAGTCTGTTCTAAGGTTAA+TGG - chr5.3:17028945-17028964 MS.gene028039:intron 30.0%
!! TTGTTTGCTTTTGTGTTTAG+AGG - chr5.3:17028915-17028934 MS.gene028039:intron 30.0%
!!! TTGTAGTTGTAGTTTTGTTG+CGG - chr5.3:17028589-17028608 MS.gene028039:CDS 30.0%
AAATGAGAATAGAGGCTCAA+GGG - chr5.3:17030338-17030357 MS.gene028039:intron 35.0%
AAATTCACTAGTTAGGGCTA+AGG + chr5.3:17030275-17030294 None:intergenic 35.0%
AATCGGACATGATACATCAT+AGG + chr5.3:17030097-17030116 None:intergenic 35.0%
ACTAGGGACAGAAGAAAATA+AGG - chr5.3:17030573-17030592 MS.gene028039:intron 35.0%
AGAGAATGGTGCACTTTAAA+GGG - chr5.3:17029193-17029212 MS.gene028039:CDS 35.0%
ATTCACAGAGAAATCCCAAT+AGG - chr5.3:17029858-17029877 MS.gene028039:intron 35.0%
TAGAGAATGGTGCACTTTAA+AGG - chr5.3:17029192-17029211 MS.gene028039:CDS 35.0%
TCATTTGAAGAGCCATTTAC+AGG - chr5.3:17028990-17029009 MS.gene028039:intron 35.0%
TTTAGAAGAGTCCTACTGTA+TGG + chr5.3:17030139-17030158 None:intergenic 35.0%
! AACTTGCAGAAGTATAGACT+TGG - chr5.3:17029303-17029322 MS.gene028039:intron 35.0%
! CTCAGCTTTGTCAAATTTCA+TGG - chr5.3:17030459-17030478 MS.gene028039:intron 35.0%
!! AACTAAAGCCTCAAGTTGAA+GGG - chr5.3:17030596-17030615 MS.gene028039:CDS 35.0%
ATCTGTAGATGGAGCTGAAA+TGG - chr5.3:17030666-17030685 MS.gene028039:CDS 40.0%
CAAACAAGCCTTTCTCAAGA+TGG - chr5.3:17030391-17030410 MS.gene028039:intron 40.0%
CAAATGAGAATAGAGGCTCA+AGG - chr5.3:17030337-17030356 MS.gene028039:intron 40.0%
CGAACTAGAGCGCAATATTT+AGG + chr5.3:17028634-17028653 None:intergenic 40.0%
GAGATTGGAAGAACAACTTG+AGG - chr5.3:17029914-17029933 MS.gene028039:intron 40.0%
GGCTTGATTGATGTATGTAG+TGG - chr5.3:17028848-17028867 MS.gene028039:intron 40.0%
TCAGGGACTAATACCAACTA+TGG - chr5.3:17029605-17029624 MS.gene028039:intron 40.0%
TGGTATTAGTCCCTGAAGAA+TGG + chr5.3:17029601-17029620 None:intergenic 40.0%
TTCACTATCACCTCCATAGT+TGG + chr5.3:17029621-17029640 None:intergenic 40.0%
! AACTATGGAGGTGATAGTGA+AGG - chr5.3:17029620-17029639 MS.gene028039:intron 40.0%
! AAGAAGAGGTACATACTGTC+AGG + chr5.3:17028542-17028561 None:intergenic 40.0%
! ACTATGGAGGTGATAGTGAA+GGG - chr5.3:17029621-17029640 MS.gene028039:intron 40.0%
! AGAAGAGGTACATACTGTCA+GGG + chr5.3:17028541-17028560 None:intergenic 40.0%
! CTATGGACTTGTGGAGTTTT+GGG - chr5.3:17028778-17028797 MS.gene028039:CDS 40.0%
! GACATTGAAGGAAAGTGTGA+TGG - chr5.3:17028736-17028755 MS.gene028039:CDS 40.0%
! TCTATGGACTTGTGGAGTTT+TGG - chr5.3:17028777-17028796 MS.gene028039:CDS 40.0%
!! ACTAAAGCCTCAAGTTGAAG+GGG - chr5.3:17030597-17030616 MS.gene028039:CDS 40.0%
!! GAACTAAAGCCTCAAGTTGA+AGG - chr5.3:17030595-17030614 MS.gene028039:CDS 40.0%
CGACTCCTAAGTCTGTTCTA+AGG - chr5.3:17028938-17028957 MS.gene028039:intron 45.0%
GACAAAGCGGTCATGAAGAT+CGG + chr5.3:17028495-17028514 None:intergenic 45.0%
GCCTTAGTGCTTCTCCTATT+GGG + chr5.3:17029875-17029894 None:intergenic 45.0%
GGGACTAATACCAACTATGG+AGG - chr5.3:17029608-17029627 MS.gene028039:intron 45.0%
TGCCTTAGTGCTTCTCCTAT+TGG + chr5.3:17029876-17029895 None:intergenic 45.0%
TGCTCTGTCCATCTTGAGAA+AGG + chr5.3:17030402-17030421 None:intergenic 45.0%
! CTATGGAGGTGATAGTGAAG+GGG - chr5.3:17029622-17029641 MS.gene028039:intron 45.0%
! GGAGGAATTGAAGGGTATGA+TGG - chr5.3:17028419-17028438 MS.gene028039:CDS 45.0%
! TATGGAGGTGATAGTGAAGG+GGG - chr5.3:17029623-17029642 MS.gene028039:intron 45.0%
!! AGAGGCTCAAGGGAAGTATT+TGG - chr5.3:17030348-17030367 MS.gene028039:intron 45.0%
!! CTAAAGCCTCAAGTTGAAGG+GGG - chr5.3:17030598-17030617 MS.gene028039:CDS 45.0%
!! TCCCAATAGGAGAAGCACTA+AGG - chr5.3:17029871-17029890 MS.gene028039:intron 45.0%
AGTCCTACTGTATGGTGCGA+AGG + chr5.3:17030131-17030150 None:intergenic 50.0%
ATTGCACCCCCTTCAACTTG+AGG + chr5.3:17030607-17030626 None:intergenic 50.0%
GAGGGAGAGGAGGAATTGAA+GGG - chr5.3:17028411-17028430 MS.gene028039:CDS 50.0%
GTCCTACTGTATGGTGCGAA+GGG + chr5.3:17030130-17030149 None:intergenic 50.0%
! ATGATGGAAAGTGGAGGGAG+AGG - chr5.3:17028398-17028417 MS.gene028039:CDS 50.0%
! ATGGAGGTGATAGTGAAGGG+GGG - chr5.3:17029624-17029643 MS.gene028039:intron 50.0%
! CGAAGGGCACAACGAACAAT+CGG + chr5.3:17030114-17030133 None:intergenic 50.0%
!! GGCTCAAGGGAAGTATTTGG+AGG - chr5.3:17030351-17030370 MS.gene028039:intron 50.0%
!! TTGTGACAGCGTCGACAAAG+CGG + chr5.3:17028508-17028527 None:intergenic 50.0%
ATGGAAAGTGGAGGGAGAGG+AGG - chr5.3:17028401-17028420 MS.gene028039:CDS 55.0%
GACGCTGTCACAAAGCTTGG+TGG - chr5.3:17028515-17028534 MS.gene028039:CDS 55.0%
GGAGGGAGAGGAGGAATTGA+AGG - chr5.3:17028410-17028429 MS.gene028039:CDS 55.0%
GTCGACGCTGTCACAAAGCT+TGG - chr5.3:17028512-17028531 MS.gene028039:CDS 55.0%
TGCCCTTCGCACCATACAGT+AGG - chr5.3:17030125-17030144 MS.gene028039:CDS 55.0%
TCGGTTGTCCACCTCAGCCT+CGG + chr5.3:17028476-17028495 None:intergenic 60.0%
GTCCACCTCAGCCTCGGCTT+CGG + chr5.3:17028470-17028489 None:intergenic 65.0%
TCCACCTCAGCCTCGGCTTC+GGG + chr5.3:17028469-17028488 None:intergenic 65.0%
TGACGAGAGACCCGAAGCCG+AGG - chr5.3:17028456-17028475 MS.gene028039:CDS 65.0%
ACCCGAAGCCGAGGCTGAGG+TGG - chr5.3:17028465-17028484 MS.gene028039:CDS 70.0%
GAGACCCGAAGCCGAGGCTG+AGG - chr5.3:17028462-17028481 MS.gene028039:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 17028398 17030726 17028398 ID=MS.gene028039
chr5.3 mRNA 17028398 17030726 17028398 ID=MS.gene028039.t1;Parent=MS.gene028039
chr5.3 exon 17030579 17030726 17030579 ID=MS.gene028039.t1.exon1;Parent=MS.gene028039.t1
chr5.3 CDS 17030579 17030726 17030579 ID=cds.MS.gene028039.t1;Parent=MS.gene028039.t1
chr5.3 exon 17030113 17030189 17030113 ID=MS.gene028039.t1.exon2;Parent=MS.gene028039.t1
chr5.3 CDS 17030113 17030189 17030113 ID=cds.MS.gene028039.t1;Parent=MS.gene028039.t1
chr5.3 exon 17029752 17029812 17029752 ID=MS.gene028039.t1.exon3;Parent=MS.gene028039.t1
chr5.3 CDS 17029752 17029812 17029752 ID=cds.MS.gene028039.t1;Parent=MS.gene028039.t1
chr5.3 exon 17029479 17029551 17029479 ID=MS.gene028039.t1.exon4;Parent=MS.gene028039.t1
chr5.3 CDS 17029479 17029551 17029479 ID=cds.MS.gene028039.t1;Parent=MS.gene028039.t1
chr5.3 exon 17029189 17029258 17029189 ID=MS.gene028039.t1.exon5;Parent=MS.gene028039.t1
chr5.3 CDS 17029189 17029258 17029189 ID=cds.MS.gene028039.t1;Parent=MS.gene028039.t1
chr5.3 exon 17028398 17028802 17028398 ID=MS.gene028039.t1.exon6;Parent=MS.gene028039.t1
chr5.3 CDS 17028398 17028802 17028398 ID=cds.MS.gene028039.t1;Parent=MS.gene028039.t1
Gene Sequence

>MS.gene028039

ATGATGGAAAGTGGAGGGAGAGGAGGAATTGAAGGGTATGATGGAATGATGATGACAATGACGAGAGACCCGAAGCCGAGGCTGAGGTGGACAACCGATCTTCATGACCGCTTTGTCGACGCTGTCACAAAGCTTGGTGGCCCTGACAGTATGTACCTCTTCTTTCATCTAGTTTTGTTGAAGTTTTTAAATTGTAGTTGTAGTTTTGTTGCGGTAGCAAAGACATAGAAAAACCTAAATATTGCGCTCTAGTTCGCAGTTGCATACCTTTATTTAAAATTTTAGTGTTTGCTACACATTTATGTTAACTTTAAAGGGAAGTTTTTTTTGTTAAAATTGACATTGAAGGAAAGTGTGATGGAGAAATGTTGAATTAATCTCTATGGACTTGTGGAGTTTTGGGCTAGTCCAATTTTGAAAGCTTATCATTCTAAATATTTTCTTATTAATGGCTTGATTGATGTATGTAGTGGTTCTTAGTTTGTCACAAAAATGATATTAATTTGAAATGTTGTTTTTGTTTGCTTTTGTGTTTAGAGGCGACTCCTAAGTCTGTTCTAAGGTTAATGGGATTAAAAGGTTTGACATTATATCATTTGAAGAGCCATTTACAGGTAACAACTTCTACCACCTTTTTAGTCAAAAAATAAAATAAAATTTCTACACCTTTTCTTTTGCCTTTTTACATAGTGATGATATATTTAATTTATGAAGGTATAAACTAGCAGTTAAAAATAGGTTGAGAAGTGAAAAGACAAATTAGTATTAGTTATAGAGATTATATTATCATTAATTAGAGAATGGTGCACTTTAAAGGGTAAACTAATTGAAAAATAAATAGTTGAAGCTTGAGTTTGTGATAAAATATGTACGTGACTTTCTATAATGAAGAACATTTTTCTTTTAACTTGCAGAAGTATAGACTTGGACAACATGCTCGAAAACAAAATGAAGAACAACTCAAAGAGAATAATAGTGAGTTCATTACAAATTTTGAAAGAGTCTCAAATAAGTGAAGGGTGTGTTTAGCTTGACTTGTTTGAGAGAGTTTATGAAAAGAGCTTATGAAAATAACTTATGATATGTTCATAACTTCATAAGCTTTTTTCAGATAACTTTCATTAGCAATTATTTCTATTACATTTGGTTGATTTTTTTGTCTTTCATCTTTTTAGGATGTTCCTATGTAAATTTCAGTAACCATTCTTCAGGGACTAATACCAACTATGGAGGTGATAGTGAAGGGGGGTATGTTTAGAATTTAATCAAAATTCACTTATTTTGCTTAATAGTTATTATTATACGTATGACTATATTTTTCATGAGCCAAATTCTCATCTCTTTCAAAAAAAAAAAAAAGAGAGAGCCAAATTCTCATCTAAAACTTCTTGCCTATGTAATTAGCATTTTAATTTTTAAGTAAATACAACTTATTATATTTCCTAACTAGTTTAACGACAATTCACAGAGAAATCCCAATAGGAGAAGCACTAAGGCAACAAATTGAAGTTCAAAAGAGATTGGAAGAACAACTTGAGGTACTCTCTCACTTTTTCTTTTACTTGCACTATAAGACACTTTTTTAAAATTTTACATGTATTAATCATTTTTTTTACAAAAATATTCCTATGTAATTGACAATTGAGAAAAGTAAATTCATTATCTTTAATTTTATTTTAAAAACATAAAAGCACTACCTATGATGTATCATGTCCGATTGTTCGTTGTGCCCTTCGCACCATACAGTAGGACTCTTCTAAAAAAATAACGCATTTTTTTTTAATAAATTTATTGCTAATATACTATTTTTTTTCGTAATACCACTAAAATTAGTATGTGAGTCTTATAATAAGAGACCAAAGGAGTATATATATCCTTAGCCCTAACTAGTGAATTTCTAACCAATTGGACTGTTTAATTCTAGGTACAAAATAAATTACAAATGAGAATAGAGGCTCAAGGGAAGTATTTGGAGGCAGTGTTAGAGAAAGCACAAACAAGCCTTTCTCAAGATGGACAGAGCAATCTTAATGCATCAAAAGCTCAATTGGTTGAGTTCAACTCAGCTTTGTCAAATTTCATGGAAAATATGAATAAAGATAGTAAAGAAAACATACTAGGCATGAATGAATTCCATAATAAGAATCATGCTCAAGCTTTCAATTATCAAGAAGTACTAGGGACAGAAGAAAATAAGGAACTAAAGCCTCAAGTTGAAGGGGGTGCAATACAGCTAGACTTAAACATTAAAGGTGGCAATGAACTTGTATCTGTAGATGGAGCTGAAATGGAATCAAATATGGTTTCATATAGGGTATACCGTTTTTGA
Protein sequence

>MS.gene028039.t1

MMESGGRGGIEGYDGMMMTMTRDPKPRLRWTTDLHDRFVDAVTKLGGPDKATPKSVLRLMGLKGLTLYHLKSHLQKYRLGQHARKQNEEQLKENNRCSYVNFSNHSSGTNTNYGGDSEGGEIPIGEALRQQIEVQKRLEEQLEVQNKLQMRIEAQGKYLEAVLEKAQTSLSQDGQSNLNASKAQLVEFNSALSNFMENMNKDSKENILGMNEFHNKNHAQAFNYQEVLGTEENKELKPQVEGGAIQLDLNIKGGNELVSVDGAEMESNMVSYRVYRF