AlfalfaGEDB | MS.gene028112

M. sativa cultivar XinJiangDaYe / MS.gene028112

KEGG: -
GO: -
PFAM: PF13837.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028112.t1	XP_003612572.1	91.7	289	21	1	1	286	1	289	1.70E-98	369.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028112.t1	O80450	55.0	262	105	4	26	286	26	275	5.8e-23	109.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028112.t1	G7K6R0	91.7	289	21	1	1	286	1	289	1.2e-98	369.4

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene028112.t1	TF	Trihelix

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028112.t1	MTR_5g026540	97.232	289	5	1	1	286	1	289	0.0	569
MS.gene028112.t1	MTR_4g015680	49.180	244	108	5	44	286	40	268	2.44e-51	171
MS.gene028112.t1	MTR_7g068770	46.538	260	124	5	28	286	35	280	2.15e-46	159
MS.gene028112.t1	MTR_3g085960	50.820	61	30	0	46	106	77	137	7.78e-14	72.0
MS.gene028112.t1	MTR_2g026725	31.356	118	70	3	30	138	241	356	4.08e-13	69.7
MS.gene028112.t1	MTR_1g098870	33.684	95	60	1	41	132	368	462	2.15e-12	67.8
MS.gene028112.t1	MTR_1g098900	33.696	92	56	3	46	133	455	545	5.57e-11	63.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028112.t1	AT2G38250	43.939	264	131	5	26	286	26	275	1.27e-46	159
MS.gene028112.t1	AT5G01380	39.643	280	140	6	26	286	33	302	1.28e-36	134
MS.gene028112.t1	AT1G13450	40.449	89	52	1	46	133	87	175	2.78e-15	74.3
MS.gene028112.t1	AT1G13450	40.449	89	52	1	46	133	87	175	5.22e-15	74.3
MS.gene028112.t1	AT1G13450	40.449	89	52	1	46	133	87	175	5.22e-15	74.3
MS.gene028112.t1	AT1G13450	40.449	89	52	1	46	133	87	175	1.44e-14	74.3
MS.gene028112.t1	AT3G25990	39.785	93	48	2	46	132	55	145	2.11e-14	73.6
MS.gene028112.t1	AT1G76890	34.711	121	74	3	19	135	371	490	1.29e-13	71.6
MS.gene028112.t1	AT1G76880	34.579	107	60	4	30	132	398	498	2.65e-11	64.7

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Find 81 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGTACGGCGGCGGTGG+AGG	0.483528	5.3:-16463273	MS.gene028112:CDS
AAACCGAAAATGTACGGCGG+CGG	0.675954	5.3:-16463279	None:intergenic
AACAGTTGTTTGAACAGGAA+TGG	0.513571	5.3:-16461262	MS.gene028112:CDS
AAGAGAGTTCATAGCGATAA+GGG	0.460403	5.3:-16463118	MS.gene028112:CDS
AAGTGGTGAGTTCGAAGATG+AGG	0.496550	5.3:-16463044	MS.gene028112:CDS
AATACACGTAAAAGGAAAGC+TGG	0.551659	5.3:-16461422	MS.gene028112:CDS
AATGATTGAACAGGAATGGA+GGG	0.570661	5.3:-16461204	MS.gene028112:CDS
AATGGAGATGCAGTGGAGGG+AGG	0.556920	5.3:-16461309	MS.gene028112:CDS
ACAGGAATGGCGGCAGTCAA+TGG	0.476521	5.3:-16461249	MS.gene028112:CDS
ACTTGCATTTACACTGTTCA+GGG	0.425547	5.3:+16463001	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACATTGAAGGAGTTTGAAA+AGG	-	chr5.3:16462322-16462341	MS.gene028112:intron	30.0%
AACCTCTAACATGTATGATT+TGG	-	chr5.3:16462470-16462489	MS.gene028112:intron	30.0%
AATTAAAAATGGAGATGCAG+TGG	-	chr5.3:16463006-16463025	MS.gene028112:CDS	30.0%
ATAAGTATGGACCATAATGT+TGG	-	chr5.3:16461951-16461970	MS.gene028112:intron	30.0%
GAAATATGCATTTGTAGTGT+TGG	-	chr5.3:16461438-16461457	MS.gene028112:CDS	30.0%
GAATCTTGTTAATCGCTATA+AGG	-	chr5.3:16461348-16461367	MS.gene028112:CDS	30.0%
GATTAATGATTGAACAGGAA+TGG	-	chr5.3:16463114-16463133	MS.gene028112:CDS	30.0%
GCATGCAATTCTTCAAAAAA+TGG	+	chr5.3:16462702-16462721	None:intergenic	30.0%
TAAGTATGGACCATAATGTT+GGG	-	chr5.3:16461952-16461971	MS.gene028112:intron	30.0%
TACCAAATCATACATGTTAG+AGG	+	chr5.3:16462475-16462494	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	16461052	16463292	16461052	ID=MS.gene028112
chr5.3	mRNA	16461052	16463292	16461052	ID=MS.gene028112.t1;Parent=MS.gene028112
chr5.3	exon	16462975	16463292	16462975	ID=MS.gene028112.t1.exon1;Parent=MS.gene028112.t1
chr5.3	CDS	16462975	16463292	16462975	ID=cds.MS.gene028112.t1;Parent=MS.gene028112.t1
chr5.3	exon	16461052	16461684	16461052	ID=MS.gene028112.t1.exon2;Parent=MS.gene028112.t1
chr5.3	CDS	16461052	16461684	16461052	ID=cds.MS.gene028112.t1;Parent=MS.gene028112.t1

Gene Sequence

>MS.gene028112

ATGTACGGCGGCGGTGGAGGAGACGATGCTCTGGCAGCTCGCCTGGGAATAGTACCACCGTCACTTCTGATTCCGACATCGTCGTTGATGTCTCCTGCGGCGGTGAGAGAGGAGAGGGTACCAGCTCAGCCGCAGTGGAGTCAACAGGAGACAAGAGAGTTCATAGCGATAAGGGCGGAGCTGGAGAGGGATTTCACTGCTTCCAAGAGGAACAAGACGCTTTGGGAAGTGGTGAGTTCGAAGATGAGGGAGAGAGGGTATAGGAGGAGCCCTGAACAGTGTAAATGCAAGTGGAAGAATCTTGTTAATCGCTATAAGGTATCTTTCTCTTTATGTTTTGGGTTTTTAGGGTTTATGATCATTTATAATCATAATTGATTGAAATTGAAATATGCATTTGTAGTGTTGGAGAAATGAGTTGCAGAAAGACATGGTTTTTGATAGAACATTATTGCGTCTTTGATTCATGCAACCACCCTACTTCCTTGGTGGGACAAGGCTTCGTTGTTGTAATGTTTAATAATGTTGTAGTACTTATCATATTTAATTGTAATGCATTGTTATAATTTCATTTGGAATATGAACTAAGAGAGTTTTCTCATAATCATATATCGCATTTTTGTTTTTAGCTTTAAGTATGATTATAGTCCTTACAATTATATCATTTTTTGTTTTCAGTACTGTTAGTGTTTTTGTTTCGATTATGTCCCTGCAAATTCAAAAACTTAGTCTCTTGGTGTCATACATGGCCCAATAAGCTGACGTTAAGGACTTAGAGAAACAATTTTTGAATTTGCAGGGACTGAATCCAAACAAAAAAAAACTTACAAAGACCGAAAGCATTAGTTTTTGAATTTATATCCAACATTTGTTTTTATTACCATTTGGACTGTGGATAAATAAGTATGGACCATAATGTTGGGTTGAGTTCATATATATGAAATGCTGTGATTTGGTTGGAGGGTGTGTTATGGATTGGGTCAGGATCCATTTGAACTGGACCTCATTATGTGAAGCCCACCCCTAACTAGAGCGATTCAGAAGCTATCCACATCACTGCACCATTATGTGCCCCAACCAACTTTCCCATCAAGAAACCTTGTCAAGCGAGATCACTCTTTCATAGTAATGATACACAACATCTCTTATCGCTCACTCAACTCGATCAAATGCTAACTTGAGCTTCGGAGTGCAAGCTCAATGCTTGAAAGTTGAAACTCACCAATATAACTTTTTAGTCCTCAAGGTGTAGTTCAATTGGTTGAGCTGAAACATTGAAGGAGTTTGAAAAGGAGGTCTAGGTTCAAACTTCGATTACTAATAAGTAACTTGTGCTTATCAAAAAACTAAGTATAGCTCCTTCAACCTGCCAAACGGCCTCACTAGTGCATATTGACCTCCCCTTTGCATATGCAGAAAACCTCTAACATGTATGATTTGGTATTTGGTGAGTTCGAGTAAATTTAATCATGTCGTTGGGTTAATATTATTGTGCAACCAACTGTGCATCAGTTTTCTTTTATTTGAAAATGCAGAAAAAAGCTATATCTTGAATATTGGGGAATGTTTGCTTTACTAAGTAAAAATCACATGATGATGTTTATGCAGGGAAAAGAGACATCTGATCCAGAACATGGAAAGCAATGCCCATTTTTTGAAGAATTGCATGCAGTTTTTACCGAAAGAGCACATAGCATGCAACGCTTACTTCTTGAATCTGAAACTCGTTCAGCTCAAACAAAAAAAGGAGTGAAGAAAATAAGAAGTGGAGACCGATCCTCTGACGAATTCTCAGAAGATGATGAGGAAGTTGAAGATGACAGTGAAGAGGAGAGACCTGCGAGAAGCAATACACGTAAAAGGAAAGCTGGCAAAATTGCAACAGAGAAGTCTTCAAGGGCAAACAATAGTACTAGTAATATTCAAGAAATGCTCAAGGAATTTTTCCAACACCAATTAAAAATGGAGATGCAGTGGAGGGAGGTGATGGAAAGGCGGGCACATGAAAAACAGTTGTTTGAACAGGAATGGCGGCAGTCAATGGAAAAGCTTGAGAGGGAGAGATTAATGATTGAACAGGAATGGAGGGAAAGAGAAGAGCATAGGAGACTGAGGGAGGAGAATAGGGCAGAGAGAAGGGACGCTTTAAATGAGTCAACTGGCTTGGATTCTTCACAGCTGATTTATTTGTTGTTTCAATTTAAATATATGATTTTAAGTTTGGCAAAGAGAATGAACTAA

Protein sequence

>MS.gene028112.t1

MYGGGGGDDALAARLGIVPPSLLIPTSSLMSPAAVREERVPAQPQWSQQETREFIAIRAELERDFTASKRNKTLWEVVSSKMRERGYRRSPEQCKCKWKNLVNRYKGKETSDPEHGKQCPFFEELHAVFTERAHSMQRLLLESETRSAQTKKGVKKIRSGDRSSDEFSEDDEEVEDDSEEERPARSNTRKRKAGKIATEKSSRANNSTSNIQEMLKEFFQHQLKMEMQWREVMERRAHEKQLFEQEWRQSMEKLERERLMIEQEWREREEHRRLREENRAERRDALNESTGLDSSQLIYLLFQFKYMILSLAKRMN