AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028214

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028214.t1 MTR_5g027020 48.711 388 151 9 5 384 7 354 2.38e-123 361
MS.gene028214.t1 MTR_1g038390 61.940 134 39 4 213 345 4 126 2.18e-40 140
MS.gene028214.t1 MTR_3g073000 25.857 321 186 11 8 313 13 296 5.22e-18 85.5
MS.gene028214.t1 MTR_3g073000 25.857 321 186 11 8 313 13 296 7.26e-18 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028214.t1 AT2G19640 52.696 408 156 10 2 389 7 397 1.41e-136 396
MS.gene028214.t1 AT2G19640 55.272 313 111 8 2 299 7 305 1.64e-110 328
MS.gene028214.t1 AT2G17900 25.589 297 174 11 8 291 18 280 3.60e-15 77.4

Find 72 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAAGAGTGATGAGAAAA+AGG 0.178869 4.2:+16460153 None:intergenic
AAAGATAAGCTTAATGCTTT+TGG 0.201202 4.2:-16459745 MS.gene028214:CDS
GGAGAATGAAGAAGAGGATA+TGG 0.278011 4.2:-16459365 MS.gene028214:CDS
AATAGGTCTGTTAGAGCTTA+TGG 0.303956 4.2:-16459691 MS.gene028214:CDS
TTTGCTGCTTCATCATTATC+AGG 0.305057 4.2:+16459853 None:intergenic
TCTCTGAAACTGATGAAAAT+AGG 0.345771 4.2:-16459708 MS.gene028214:CDS
GGAGGAGCAATGTGAAAATA+TGG 0.349748 4.2:-16459320 MS.gene028214:CDS
AAGAGATTGTTGGAAGACTA+TGG 0.351805 4.2:-16459463 MS.gene028214:CDS
AACGAAAACAATTCATTATG+AGG 0.359381 4.2:+16459793 None:intergenic
TTGAATTGCAAAATCGATAT+CGG 0.369503 4.2:+16460070 None:intergenic
GATGTAATGTTGAATCGAAT+TGG 0.384827 4.2:-16459420 MS.gene028214:CDS
GAAGCAAAAGAGGATTGCTT+TGG 0.410291 4.2:+16459983 None:intergenic
TAATCAAATCTACAAGCATT+AGG 0.420326 4.2:+16459628 None:intergenic
GAAATTCAAGGTAGAGGAAG+AGG 0.427064 4.2:-16460252 MS.gene028214:CDS
TCTACCTTCAAAACAGAACT+TGG 0.428323 4.2:+16460276 None:intergenic
TAACAAAACTTGAAAATTTG+AGG 0.434129 4.2:+16459891 None:intergenic
ACTTGAATGTTGATGATAAA+GGG 0.440641 4.2:-16459126 MS.gene028214:CDS
TATGGATGATGAGGAGGAGG+AGG 0.450793 4.2:-16459341 MS.gene028214:CDS
GACTTGAATGTTGATGATAA+AGG 0.457168 4.2:-16459127 MS.gene028214:CDS
AGACCGGCCGCGACATCATA+AGG 0.460613 4.2:+16459589 None:intergenic
GTATCACCTTGTGGCGGTAA+AGG 0.461262 4.2:+16459205 None:intergenic
TGCTTGAAGTTGTCTTTCGA+GGG 0.463012 4.2:+16459954 None:intergenic
TTAATGAAAACTATTCTAGT+AGG 0.465091 4.2:-16459489 MS.gene028214:CDS
GGATGATGAGGAGGAGGAGG+AGG 0.480114 4.2:-16459338 MS.gene028214:CDS
AACAAAACTTGAAAATTTGA+GGG 0.484733 4.2:+16459892 None:intergenic
GGCTATGGATGATGAGGAGG+AGG 0.485383 4.2:-16459344 MS.gene028214:CDS
GTGCTTGAAGTTGTCTTTCG+AGG 0.486050 4.2:+16459953 None:intergenic
AGGGGTTGTTGAAGCAAAAG+AGG 0.497882 4.2:+16459973 None:intergenic
TAGTAGGCAGAAGAGATTGT+TGG 0.499496 4.2:-16459473 MS.gene028214:CDS
GTAGAGGAAATTCAAGGTAG+AGG 0.507246 4.2:-16460258 MS.gene028214:CDS
TTGAAGGTAGAGGAAATTCA+AGG 0.513971 4.2:-16460264 MS.gene028214:CDS
AAAATCAGTATTACTAAGAC+CGG 0.525101 4.2:+16459573 None:intergenic
ACTGATAATGAGAGTGTTGA+AGG 0.527867 4.2:-16459397 MS.gene028214:CDS
GCATCTCAACCTCTCAAAGC+TGG 0.531187 4.2:-16460222 MS.gene028214:CDS
ACAAAACTTGAAAATTTGAG+GGG 0.531312 4.2:+16459893 None:intergenic
GTGGTAGGGTAAAGGAGGAT+TGG 0.532177 4.2:+16460177 None:intergenic
TGAAGAAGAGGATATGGCTA+TGG 0.532611 4.2:-16459359 MS.gene028214:CDS
CCACCTTATGATGTCGCGGC+CGG 0.533777 4.2:-16459592 MS.gene028214:CDS
TGTGTGATAGAACAAATTGC+TGG 0.536848 4.2:-16459240 MS.gene028214:CDS
TGTGGAGGAGAATGAAGAAG+AGG 0.542011 4.2:-16459371 MS.gene028214:CDS
TCGTTATTGTCAGTTTCTGA+TGG 0.542245 4.2:+16459295 None:intergenic
GGATATGGCTATGGATGATG+AGG 0.556063 4.2:-16459350 MS.gene028214:CDS
GGGATGCAATAATGTGGTGT+AGG 0.556795 4.2:+16459827 None:intergenic
AAGCATTAGGAAGACAATCA+TGG 0.557161 4.2:+16459641 None:intergenic
TGATACATTATCTAATGTCA+TGG 0.566313 4.2:-16459188 MS.gene028214:CDS
TCATGATGTTCCACAAGGTA+GGG 0.572819 4.2:-16459536 MS.gene028214:CDS
TATGGCTATGGATGATGAGG+AGG 0.579991 4.2:-16459347 MS.gene028214:CDS
ACGAAAACAATTCATTATGA+GGG 0.582255 4.2:+16459794 None:intergenic
TGAGAGTGTTGAAGGAAATG+TGG 0.582356 4.2:-16459389 MS.gene028214:CDS
GTTGATGATAAAGGGCAAAG+TGG 0.587949 4.2:-16459118 MS.gene028214:CDS
ATGATTTGACCAGCTTTGAG+AGG 0.589051 4.2:+16460213 None:intergenic
AAGGGGTGGTAGGGTAAAGG+AGG 0.590878 4.2:+16460172 None:intergenic
GTGTGATAGAACAAATTGCT+GGG 0.601867 4.2:-16459239 MS.gene028214:CDS
AAACAAATCTCCCTACCTTG+TGG 0.609840 4.2:+16459526 None:intergenic
TTAGCTCCTTTACCGCCACA+AGG 0.628714 4.2:-16459211 MS.gene028214:CDS
TCATGGAATGTAATGTCTGT+GGG 0.630100 4.2:-16459171 MS.gene028214:CDS
TTAGATAATGTATCACCTTG+TGG 0.636379 4.2:+16459196 None:intergenic
GTCATGGAATGTAATGTCTG+TGG 0.637762 4.2:-16459172 MS.gene028214:CDS
TTCATGATGTTCCACAAGGT+AGG 0.641511 4.2:-16459537 MS.gene028214:CDS
GCTTGAAGTTGTCTTTCGAG+GGG 0.645950 4.2:+16459955 None:intergenic
GCAAAGTGGAGAAGTTCACA+TGG 0.648528 4.2:-16459104 MS.gene028214:CDS
TAATCCACCTTATGATGTCG+CGG 0.663274 4.2:-16459596 MS.gene028214:CDS
GAGTGTTGAAGGAAATGTGG+AGG 0.667098 4.2:-16459386 MS.gene028214:CDS
TCAACTCATCACATTCCACA+TGG 0.667873 4.2:-16460029 MS.gene028214:CDS
CAAAACTTGAAAATTTGAGG+GGG 0.669304 4.2:+16459894 None:intergenic
GGAGGATTGGTGAATCTGTG+AGG 0.693945 4.2:+16460190 None:intergenic
ATGATTCATGATGTTCCACA+AGG 0.697653 4.2:-16459541 MS.gene028214:CDS
CCGGCCGCGACATCATAAGG+TGG 0.702146 4.2:+16459592 None:intergenic
GATAATGTATCACCTTGTGG+CGG 0.702824 4.2:+16459199 None:intergenic
TAATGCTTGACAAATCCATG+TGG 0.720035 4.2:+16460014 None:intergenic
TGTGATAGAACAAATTGCTG+GGG 0.747351 4.2:-16459238 MS.gene028214:CDS
AAGTGGAGAAGTTCACATGG+AGG 0.760776 4.2:-16459101 MS.gene028214:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAACTTGAAAATTTGA+GGG + chr4.2:16459492-16459511 None:intergenic 20.0%
!! TAACAAAACTTGAAAATTTG+AGG + chr4.2:16459493-16459512 None:intergenic 20.0%
!! TTAATGAAAACTATTCTAGT+AGG - chr4.2:16459892-16459911 MS.gene028214:CDS 20.0%
!!! GTAATACTGATTTTGTTATT+AGG - chr4.2:16459817-16459836 MS.gene028214:CDS 20.0%
! AAAATCAGTATTACTAAGAC+CGG + chr4.2:16459811-16459830 None:intergenic 25.0%
! AACGAAAACAATTCATTATG+AGG + chr4.2:16459591-16459610 None:intergenic 25.0%
! ACAAAACTTGAAAATTTGAG+GGG + chr4.2:16459491-16459510 None:intergenic 25.0%
! ACGAAAACAATTCATTATGA+GGG + chr4.2:16459590-16459609 None:intergenic 25.0%
! TAATCAAATCTACAAGCATT+AGG + chr4.2:16459756-16459775 None:intergenic 25.0%
! TCAAAAAGAAATAAGCATGT+GGG + chr4.2:16460115-16460134 None:intergenic 25.0%
! TGATACATTATCTAATGTCA+TGG - chr4.2:16460193-16460212 MS.gene028214:CDS 25.0%
! TTCAAAAAGAAATAAGCATG+TGG + chr4.2:16460116-16460135 None:intergenic 25.0%
! TTGAATTGCAAAATCGATAT+CGG + chr4.2:16459314-16459333 None:intergenic 25.0%
!! AAAGATAAGCTTAATGCTTT+TGG - chr4.2:16459636-16459655 MS.gene028214:CDS 25.0%
!! CTAGAATAGTTTTCATTAAC+AGG + chr4.2:16459891-16459910 None:intergenic 25.0%
!!! ACTTGAATGTTGATGATAAA+GGG - chr4.2:16460255-16460274 MS.gene028214:CDS 25.0%
AAAAAAGGGATGCAATAATG+TGG + chr4.2:16459563-16459582 None:intergenic 30.0%
AAAGAAGAGTGATGAGAAAA+AGG + chr4.2:16459231-16459250 None:intergenic 30.0%
AAGAAGAGTGATGAGAAAAA+GGG + chr4.2:16459230-16459249 None:intergenic 30.0%
CAAAACTTGAAAATTTGAGG+GGG + chr4.2:16459490-16459509 None:intergenic 30.0%
GATGTAATGTTGAATCGAAT+TGG - chr4.2:16459961-16459980 MS.gene028214:CDS 30.0%
TTAGATAATGTATCACCTTG+TGG + chr4.2:16460188-16460207 None:intergenic 30.0%
! TTTTCATCAGTTTCAGAGAA+AGG + chr4.2:16459672-16459691 None:intergenic 30.0%
!! GACTTGAATGTTGATGATAA+AGG - chr4.2:16460254-16460273 MS.gene028214:CDS 30.0%
!! TCTCTGAAACTGATGAAAAT+AGG - chr4.2:16459673-16459692 MS.gene028214:CDS 30.0%
!!! CAAGTTTTGTTATCTCTACA+AGG - chr4.2:16459501-16459520 MS.gene028214:CDS 30.0%
AAGAGATTGTTGGAAGACTA+TGG - chr4.2:16459918-16459937 MS.gene028214:CDS 35.0%
AAGCATTAGGAAGACAATCA+TGG + chr4.2:16459743-16459762 None:intergenic 35.0%
AATAGGTCTGTTAGAGCTTA+TGG - chr4.2:16459690-16459709 MS.gene028214:CDS 35.0%
AGAAGAGTGATGAGAAAAAG+GGG + chr4.2:16459229-16459248 None:intergenic 35.0%
ATGATTCATGATGTTCCACA+AGG - chr4.2:16459840-16459859 MS.gene028214:CDS 35.0%
GGTTGAAAAACGAAGCATAA+GGG + chr4.2:16459722-16459741 None:intergenic 35.0%
GTGTGATAGAACAAATTGCT+GGG - chr4.2:16460142-16460161 MS.gene028214:CDS 35.0%
TAATGCTTGACAAATCCATG+TGG + chr4.2:16459370-16459389 None:intergenic 35.0%
TCATGGAATGTAATGTCTGT+GGG - chr4.2:16460210-16460229 MS.gene028214:CDS 35.0%
TCGTTATTGTCAGTTTCTGA+TGG + chr4.2:16460089-16460108 None:intergenic 35.0%
TCTACCTTCAAAACAGAACT+TGG + chr4.2:16459108-16459127 None:intergenic 35.0%
TGGTTGAAAAACGAAGCATA+AGG + chr4.2:16459723-16459742 None:intergenic 35.0%
TGTGATAGAACAAATTGCTG+GGG - chr4.2:16460143-16460162 MS.gene028214:CDS 35.0%
TGTGTGATAGAACAAATTGC+TGG - chr4.2:16460141-16460160 MS.gene028214:CDS 35.0%
TTGAAGGTAGAGGAAATTCA+AGG - chr4.2:16459117-16459136 MS.gene028214:CDS 35.0%
! ATTATGAGGGTGCGAAAAAA+GGG + chr4.2:16459577-16459596 None:intergenic 35.0%
! TTTGCTGCTTCATCATTATC+AGG + chr4.2:16459531-16459550 None:intergenic 35.0%
!! ACTGATAATGAGAGTGTTGA+AGG - chr4.2:16459984-16460003 MS.gene028214:CDS 35.0%
!! TGTTCCAAGTTCTGTTTTGA+AGG - chr4.2:16459101-16459120 MS.gene028214:CDS 35.0%
!!! AAGTTCTGTTTTGAAGGTAG+AGG - chr4.2:16459107-16459126 MS.gene028214:CDS 35.0%
AAACAAATCTCCCTACCTTG+TGG + chr4.2:16459858-16459877 None:intergenic 40.0%
AACTTGAAAATTTGAGGGGG+CGG + chr4.2:16459487-16459506 None:intergenic 40.0%
GAAATTCAAGGTAGAGGAAG+AGG - chr4.2:16459129-16459148 MS.gene028214:CDS 40.0%
GAAGCAAAAGAGGATTGCTT+TGG + chr4.2:16459401-16459420 None:intergenic 40.0%
GATAATGTATCACCTTGTGG+CGG + chr4.2:16460185-16460204 None:intergenic 40.0%
GGAGAATGAAGAAGAGGATA+TGG - chr4.2:16460016-16460035 MS.gene028214:CDS 40.0%
GGAGGAGCAATGTGAAAATA+TGG - chr4.2:16460061-16460080 MS.gene028214:CDS 40.0%
GTAGAGGAAATTCAAGGTAG+AGG - chr4.2:16459123-16459142 MS.gene028214:CDS 40.0%
GTCATGGAATGTAATGTCTG+TGG - chr4.2:16460209-16460228 MS.gene028214:CDS 40.0%
TAATCCACCTTATGATGTCG+CGG - chr4.2:16459785-16459804 MS.gene028214:CDS 40.0%
TAGTAGGCAGAAGAGATTGT+TGG - chr4.2:16459908-16459927 MS.gene028214:CDS 40.0%
TCAACTCATCACATTCCACA+TGG - chr4.2:16459352-16459371 MS.gene028214:CDS 40.0%
TCATGATGTTCCACAAGGTA+GGG - chr4.2:16459845-16459864 MS.gene028214:CDS 40.0%
TGAAGAAGAGGATATGGCTA+TGG - chr4.2:16460022-16460041 MS.gene028214:CDS 40.0%
TGCTTGAAGTTGTCTTTCGA+GGG + chr4.2:16459430-16459449 None:intergenic 40.0%
TTCATGATGTTCCACAAGGT+AGG - chr4.2:16459844-16459863 MS.gene028214:CDS 40.0%
! CATTATGAGGGTGCGAAAAA+AGG + chr4.2:16459578-16459597 None:intergenic 40.0%
!! ATGATTTGACCAGCTTTGAG+AGG + chr4.2:16459171-16459190 None:intergenic 40.0%
!! GTTGATGATAAAGGGCAAAG+TGG - chr4.2:16460263-16460282 MS.gene028214:CDS 40.0%
!! TGAGAGTGTTGAAGGAAATG+TGG - chr4.2:16459992-16460011 MS.gene028214:CDS 40.0%
AAAAAGGGGTGGTAGGGTAA+AGG + chr4.2:16459215-16459234 None:intergenic 45.0%
AAGTGGAGAAGTTCACATGG+AGG - chr4.2:16460280-16460299 MS.gene028214:CDS 45.0%
AGAGTGATGAGAAAAAGGGG+TGG + chr4.2:16459226-16459245 None:intergenic 45.0%
AGGGGTTGTTGAAGCAAAAG+AGG + chr4.2:16459411-16459430 None:intergenic 45.0%
GATGAGAAAAAGGGGTGGTA+GGG + chr4.2:16459221-16459240 None:intergenic 45.0%
GCAAAGTGGAGAAGTTCACA+TGG - chr4.2:16460277-16460296 MS.gene028214:CDS 45.0%
GCTTGAAGTTGTCTTTCGAG+GGG + chr4.2:16459429-16459448 None:intergenic 45.0%
GGATATGGCTATGGATGATG+AGG - chr4.2:16460031-16460050 MS.gene028214:CDS 45.0%
GGGATGCAATAATGTGGTGT+AGG + chr4.2:16459557-16459576 None:intergenic 45.0%
GTGCTTGAAGTTGTCTTTCG+AGG + chr4.2:16459431-16459450 None:intergenic 45.0%
TATGGCTATGGATGATGAGG+AGG - chr4.2:16460034-16460053 MS.gene028214:CDS 45.0%
TGATGAGAAAAAGGGGTGGT+AGG + chr4.2:16459222-16459241 None:intergenic 45.0%
TGTGGAGGAGAATGAAGAAG+AGG - chr4.2:16460010-16460029 MS.gene028214:CDS 45.0%
!! GAGTGTTGAAGGAAATGTGG+AGG - chr4.2:16459995-16460014 MS.gene028214:CDS 45.0%
GCATCTCAACCTCTCAAAGC+TGG - chr4.2:16459159-16459178 MS.gene028214:CDS 50.0%
GTATCACCTTGTGGCGGTAA+AGG + chr4.2:16460179-16460198 None:intergenic 50.0%
GTGGTAGGGTAAAGGAGGAT+TGG + chr4.2:16459207-16459226 None:intergenic 50.0%
TATGGATGATGAGGAGGAGG+AGG - chr4.2:16460040-16460059 MS.gene028214:CDS 50.0%
TTAGCTCCTTTACCGCCACA+AGG - chr4.2:16460170-16460189 MS.gene028214:CDS 50.0%
! GGAGGATTGGTGAATCTGTG+AGG + chr4.2:16459194-16459213 None:intergenic 50.0%
AAGGGGTGGTAGGGTAAAGG+AGG + chr4.2:16459212-16459231 None:intergenic 55.0%
GGCTATGGATGATGAGGAGG+AGG - chr4.2:16460037-16460056 MS.gene028214:CDS 55.0%
AGACCGGCCGCGACATCATA+AGG + chr4.2:16459795-16459814 None:intergenic 60.0%
CCACCTTATGATGTCGCGGC+CGG - chr4.2:16459789-16459808 MS.gene028214:CDS 60.0%
GGATGATGAGGAGGAGGAGG+AGG - chr4.2:16460043-16460062 MS.gene028214:CDS 60.0%
CCGGCCGCGACATCATAAGG+TGG + chr4.2:16459792-16459811 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 16459096 16460307 16459096 ID=MS.gene028214
chr4.2 mRNA 16459096 16460307 16459096 ID=MS.gene028214.t1;Parent=MS.gene028214
chr4.2 exon 16459096 16460307 16459096 ID=MS.gene028214.t1.exon1;Parent=MS.gene028214.t1
chr4.2 CDS 16459096 16460307 16459096 ID=cds.MS.gene028214.t1;Parent=MS.gene028214.t1
Gene Sequence

>MS.gene028214

ATGGCTGTTCCAAGTTCTGTTTTGAAGGTAGAGGAAATTCAAGGTAGAGGAAGAGGAATTGTTGCATCTCAACCTCTCAAAGCTGGTCAAATCATCCTCACAGATTCACCAATCCTCCTTTACCCTACCACCCCTTTTTCTCATCACTCTTCTTTATTTTGTGATCATTGCTTCAAATCTTTACATTCATCATTTGTTTCATGTTCTTCTTGCAACCGATATCGATTTTGCAATTCAACTTGTCTCTCAAAATCACTCAACTCATCACATTCCACATGGATTTGTCAAGCATTATCTCAGCTCCAAAGCAATCCTCTTTTGCTTCAACAACCCCTCGAAAGACAACTTCAAGCACGTTTTCTCATTGCTGCTTACAATCTTGCTATTACCGCCCCCTCAAATTTTCAAGTTTTGTTATCTCTACAAGGCTCTCCTGATAATGATGAAGCAGCAAACTTCCTACACCACATTATTGCATCCCTTTTTTCGCACCCTCATAATGAATTGTTTTCGTTACAACTTACTTCTTCGCTGCTTGCTAAAGATAAGCTTAATGCTTTTGGATTAATGCATCCTTTCTCTGAAACTGATGAAAATAGGTCTGTTAGAGCTTATGGCATTTACCCTTATGCTTCGTTTTTCAACCATGATTGTCTTCCTAATGCTTGTAGATTTGATTATGTTGATGTTAATCCACCTTATGATGTCGCGGCCGGTCTTAGTAATACTGATTTTGTTATTAGGATGATTCATGATGTTCCACAAGGTAGGGAGATTTGTTTGAGTTATTTTCCTGTTAATGAAAACTATTCTAGTAGGCAGAAGAGATTGTTGGAAGACTATGGTTTTGCGTGTAATTGTGATCGATGTAATGTTGAATCGAATTGGACTGATAATGAGAGTGTTGAAGGAAATGTGGAGGAGAATGAAGAAGAGGATATGGCTATGGATGATGAGGAGGAGGAGGAGGAGCAATGTGAAAATATGGAACCATCAGAAACTGACAATAACGATTTCCCACATGCTTATTTCTTTTTGAAATACATGTGTGATAGAACAAATTGCTGGGGAACTTTAGCTCCTTTACCGCCACAAGGTGATACATTATCTAATGTCATGGAATGTAATGTCTGTGGGAAATTGAAGAGAGACGATGACGACTTGAATGTTGATGATAAAGGGCAAAGTGGAGAAGTTCACATGGAGGACTGA
Protein sequence

>MS.gene028214.t1

MAVPSSVLKVEEIQGRGRGIVASQPLKAGQIILTDSPILLYPTTPFSHHSSLFCDHCFKSLHSSFVSCSSCNRYRFCNSTCLSKSLNSSHSTWICQALSQLQSNPLLLQQPLERQLQARFLIAAYNLAITAPSNFQVLLSLQGSPDNDEAANFLHHIIASLFSHPHNELFSLQLTSSLLAKDKLNAFGLMHPFSETDENRSVRAYGIYPYASFFNHDCLPNACRFDYVDVNPPYDVAAGLSNTDFVIRMIHDVPQGREICLSYFPVNENYSSRQKRLLEDYGFACNCDRCNVESNWTDNESVEGNVEENEEEDMAMDDEEEEEEQCENMEPSETDNNDFPHAYFFLKYMCDRTNCWGTLAPLPPQGDTLSNVMECNVCGKLKRDDDDLNVDDKGQSGEVHMED