AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028280.t1 MTR_4g094680 96.732 153 4 1 1 152 1 153 5.67e-108 307
MS.gene028280.t1 MTR_2g036170 73.134 134 25 2 21 154 36 158 5.60e-65 199
MS.gene028280.t1 MTR_4g116300 59.259 135 50 1 20 154 4 133 3.34e-53 169
MS.gene028280.t1 MTR_2g055210 54.478 134 55 1 19 152 99 226 1.67e-48 159
MS.gene028280.t1 MTR_4g078170 45.113 133 67 2 22 153 15 142 3.25e-36 124
MS.gene028280.t1 MTR_5g030130 44.526 137 70 2 19 154 32 163 1.26e-35 122
MS.gene028280.t1 MTR_8g066820 42.857 140 74 2 15 153 26 160 4.06e-35 122
MS.gene028280.t1 MTR_5g030130 42.857 140 74 2 15 153 28 162 1.17e-34 121
MS.gene028280.t1 MTR_5g007230 42.857 133 70 2 22 153 21 148 8.36e-34 118
MS.gene028280.t1 MTR_7g102790 43.704 135 67 3 22 152 7 136 2.35e-30 109
MS.gene028280.t1 MTR_1g062160 41.045 134 72 3 22 153 7 135 2.05e-29 107
MS.gene028280.t1 MTR_2g084495 41.045 134 73 4 24 151 6 139 2.59e-24 94.4
MS.gene028280.t1 MTR_2g060540 39.416 137 77 4 24 154 12 148 1.18e-23 90.5
MS.gene028280.t1 MTR_2g084525 37.956 137 79 4 24 154 6 142 3.34e-23 91.7
MS.gene028280.t1 MTR_2g084510 35.766 137 82 4 24 154 5 141 6.88e-22 88.2
MS.gene028280.t1 MTR_2g060500 38.235 136 78 4 24 153 12 147 7.20e-22 85.9
MS.gene028280.t1 MTR_8g105930 32.432 148 83 4 24 155 15 161 2.76e-19 81.3
MS.gene028280.t1 MTR_5g077740 30.065 153 95 4 5 151 2 148 7.17e-16 71.2
MS.gene028280.t1 MTR_8g097310 39.796 98 48 4 60 152 4 95 7.74e-14 65.1
MS.gene028280.t1 MTR_1g032880 39.326 89 48 3 66 152 26 110 1.60e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028280.t1 AT1G31040 73.134 134 30 1 21 154 18 145 4.22e-70 211
MS.gene028280.t1 AT3G60670 59.124 137 51 1 16 152 1 132 8.46e-55 173
MS.gene028280.t1 AT2G12646 57.037 135 53 1 20 154 4 133 4.56e-50 161
MS.gene028280.t1 AT2G12646 57.037 135 53 1 20 154 4 133 4.56e-50 161
MS.gene028280.t1 AT2G12646 56.716 134 53 1 21 154 2 130 2.54e-49 159
MS.gene028280.t1 AT2G01818 45.139 144 74 2 17 155 6 149 2.04e-37 127
MS.gene028280.t1 AT2G27930 44.366 142 73 3 20 155 2 143 1.49e-33 116
MS.gene028280.t1 AT2G27930 44.366 142 73 3 20 155 2 143 1.49e-33 116
MS.gene028280.t1 AT1G32700 44.697 132 67 2 22 152 12 138 5.72e-33 116
MS.gene028280.t1 AT4G17900 44.118 136 69 3 19 152 31 161 2.48e-32 115
MS.gene028280.t1 AT4G17900 44.118 136 69 3 19 152 17 147 3.90e-32 114
MS.gene028280.t1 AT2G27930 44.604 139 71 3 20 152 2 140 5.62e-32 113
MS.gene028280.t1 AT2G27930 44.604 139 71 3 20 152 2 140 5.62e-32 113
MS.gene028280.t1 AT1G21000 43.182 132 69 2 22 152 13 139 1.18e-31 113
MS.gene028280.t1 AT1G21000 43.182 132 69 2 22 152 16 142 1.20e-31 114
MS.gene028280.t1 AT1G76590 43.182 132 69 2 22 152 18 144 2.21e-30 110
MS.gene028280.t1 AT5G46710 41.007 139 75 3 19 155 25 158 5.76e-29 104
MS.gene028280.t1 AT1G43000 40.909 132 72 2 22 152 16 142 2.63e-28 104
MS.gene028280.t1 AT1G43000 40.909 132 72 2 22 152 9 135 2.90e-28 104
MS.gene028280.t1 AT5G46710 41.912 136 72 3 19 152 25 155 3.81e-28 104
MS.gene028280.t1 AT5G46710 41.912 136 72 3 19 152 9 139 4.12e-28 103
MS.gene028280.t1 AT1G32700 45.192 104 51 2 50 152 1 99 1.02e-23 91.7
MS.gene028280.t1 AT2G01818 48.750 80 40 1 77 155 6 85 2.03e-20 82.4
MS.gene028280.t1 AT2G27930 47.059 85 41 1 72 152 59 143 2.56e-18 77.8
MS.gene028280.t1 AT2G27930 47.059 85 41 1 72 152 59 143 2.56e-18 77.8

Find 50 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCGTTCTTCCTTTGGTTC+TGG 0.291074 4.2:-17145973 None:intergenic
TCTCCCTTTGCTTCTGATGA+TGG 0.298330 4.2:-17145875 None:intergenic
TTAATGGGTGAGACATTCTT+TGG 0.304959 4.2:+17145935 MS.gene028280:CDS
TTCGCACCGTTAATCGTATA+TGG 0.314823 4.2:-17148431 None:intergenic
TCCCTCTCATCCCCTCCTTC+AGG 0.344793 4.2:+17146054 MS.gene028280:CDS
AGACATTCTTTGGGCGTTGT+GGG 0.349334 4.2:+17145945 MS.gene028280:CDS
CACTTGTGACAGGATTCTTC+AGG 0.373696 4.2:+17148532 MS.gene028280:CDS
TATTTGTTTGACCTGAATAT+AGG 0.411844 4.2:-17148251 None:intergenic
CATCGATTGCTCCTATATTC+AGG 0.420826 4.2:+17148240 MS.gene028280:CDS
CTCCCTTTGCTTCTGATGAT+GGG 0.428222 4.2:-17145874 None:intergenic
AGAGAGACAGTGAGGGCAAA+TGG 0.430370 4.2:-17146026 None:intergenic
CGTTCTTCCTTTGGTTCTGG+TGG 0.436735 4.2:-17145970 None:intergenic
TGCATGGTTAGAGAGGTTAA+TGG 0.448349 4.2:+17145919 MS.gene028280:CDS
TTACCATGACGTGGTTCAAT+TGG 0.448712 4.2:+17148201 MS.gene028280:CDS
ACTCTTGCTGCACTTGTGAC+AGG 0.455565 4.2:+17148522 MS.gene028280:CDS
TAATGGGTGAGACATTCTTT+GGG 0.465505 4.2:+17145936 MS.gene028280:CDS
TGCATGAGAGAGAACAGAAA+TGG 0.467641 4.2:-17148562 None:intergenic
GGGTGAGACATGAGAGGAAG+TGG 0.470262 4.2:+17145845 None:intergenic
GCATGGTTAGAGAGGTTAAT+GGG 0.474331 4.2:+17145920 MS.gene028280:CDS
GAGACATTCTTTGGGCGTTG+TGG 0.480485 4.2:+17145944 MS.gene028280:CDS
ATGCAGCCATATACGATTAA+CGG 0.494856 4.2:+17148425 MS.gene028280:intron
ACCTGAAGGAGGGGATGAGA+GGG 0.503723 4.2:-17146055 None:intergenic
TACCATGACGTGGTTCAATT+GGG 0.505968 4.2:+17148202 MS.gene028280:CDS
GTCACAAGTGCAGCAAGAGT+TGG 0.556394 4.2:-17148519 None:intergenic
TACCTGAAGGAGGGGATGAG+AGG 0.560570 4.2:-17146056 None:intergenic
TGACCCATCATCAGAAGCAA+AGG 0.564164 4.2:+17145871 MS.gene028280:CDS
TAAATGAGAGGGTGCAGTCA+AGG 0.569301 4.2:+17148465 MS.gene028280:CDS
GACCCATCATCAGAAGCAAA+GGG 0.572363 4.2:+17145872 MS.gene028280:CDS
GACATTCTTTGGGCGTTGTG+GGG 0.576836 4.2:+17145946 MS.gene028280:CDS
TTTCTGTTCTCTCTCATGCA+AGG 0.584910 4.2:+17148565 MS.gene028280:CDS
TGAGAGAGAACAGAAATGGA+AGG 0.587138 4.2:-17148558 None:intergenic
GCCATATTGTGCAGGTGAGA+AGG 0.589373 4.2:+17148173 MS.gene028280:intron
ATTAACCTCTCTAACCATGC+AGG 0.589991 4.2:-17145917 None:intergenic
AGTGATATTCTTAAATGAGA+GGG 0.594917 4.2:+17148454 MS.gene028280:CDS
GTTACTAGTACCTGAAGGAG+GGG 0.599140 4.2:-17146064 None:intergenic
GTGGGGTCCACCAGAACCAA+AGG 0.600178 4.2:+17145963 MS.gene028280:CDS
AAGTGATATTCTTAAATGAG+AGG 0.602594 4.2:+17148453 MS.gene028280:CDS
AGAAACCTGCATGGTTAGAG+AGG 0.605580 4.2:+17145912 MS.gene028280:CDS
AGTTACTAGTACCTGAAGGA+GGG 0.618175 4.2:-17146065 None:intergenic
AGTGCAGCAAGAGTTGGCAT+TGG 0.622670 4.2:-17148513 None:intergenic
AAGTTACTAGTACCTGAAGG+AGG 0.625782 4.2:-17146066 None:intergenic
CACCCAATTGAACCACGTCA+TGG 0.626928 4.2:-17148204 None:intergenic
GATGAGAAGAGAGACAGTGA+GGG 0.627570 4.2:-17146033 None:intergenic
GAGAGAGAACAGAAATGGAA+GGG 0.627838 4.2:-17148557 None:intergenic
GGATGAGAAGAGAGACAGTG+AGG 0.645133 4.2:-17146034 None:intergenic
GTGCAGTCAAGGTCATGCAA+AGG 0.664808 4.2:+17148476 MS.gene028280:CDS
AAGTGAATCAGAAACCTGCA+TGG 0.669083 4.2:+17145903 MS.gene028280:CDS
TGAGACAACAGTGCAAACAA+AGG 0.686217 4.2:-17146003 None:intergenic
AAGGTATGTTTACCATGACG+TGG 0.686416 4.2:+17148192 MS.gene028280:CDS
GTTGTGGGGTGAGACATGAG+AGG 0.704900 4.2:+17145839 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTATTATTATTATTAT+AGG + chr4.2:17146747-17146766 MS.gene028280:intron 0.0%
!!! TATTTTATTTTTATTTTTTT+CGG + chr4.2:17147326-17147345 MS.gene028280:intron 0.0%
!!! TTAATTTAATTTTTATTTTT+TGG + chr4.2:17146814-17146833 MS.gene028280:intron 0.0%
!! AAATATTATTAAATTTGGTT+CGG - chr4.2:17147865-17147884 None:intergenic 10.0%
!! CAAATTTAATAATATTTCAT+TGG + chr4.2:17147868-17147887 MS.gene028280:intron 10.0%
!! CAATGAAATATTATTAAATT+TGG - chr4.2:17147870-17147889 None:intergenic 10.0%
!! TAAATATAGTTAATCTTATA+CGG - chr4.2:17147669-17147688 None:intergenic 10.0%
!! TGTAATAAAGAATAATTTAA+TGG + chr4.2:17147406-17147425 MS.gene028280:intron 10.0%
!!! AATTAAGTTTAATTTTGTAA+AGG + chr4.2:17146512-17146531 MS.gene028280:intron 10.0%
!!! CAAAATAAATATTTTTGTTA+AGG + chr4.2:17147720-17147739 MS.gene028280:intron 10.0%
!!! CTTAACAAAAATATTTATTT+TGG - chr4.2:17147722-17147741 None:intergenic 10.0%
!!! GAATTTTTTATTTAAACATT+TGG + chr4.2:17146548-17146567 MS.gene028280:intron 10.0%
!!! GATTTTATTTTATTTTCTTA+AGG - chr4.2:17147828-17147847 None:intergenic 10.0%
!!! TTATTTTTGATAAATTTGAT+AGG + chr4.2:17146355-17146374 MS.gene028280:intron 10.0%
!! AAAAGAAGTAATAGAAATTA+AGG - chr4.2:17146167-17146186 None:intergenic 15.0%
!! AATAGAATAAAGTAATCTAA+AGG - chr4.2:17146211-17146230 None:intergenic 15.0%
!! ATTAACAAACTCTATTTAAT+GGG - chr4.2:17147236-17147255 None:intergenic 15.0%
!! TAAAAATAAAAACTAGAGTT+GGG - chr4.2:17147161-17147180 None:intergenic 15.0%
!! TAATTGAAGAACATATATAT+AGG - chr4.2:17146257-17146276 None:intergenic 15.0%
!!! AATTTTGTAAAACAACTTAT+AGG + chr4.2:17147640-17147659 MS.gene028280:intron 15.0%
!!! ATATACTGATATTGATTTTT+GGG - chr4.2:17148308-17148327 None:intergenic 15.0%
!!! TAATTTCTACTTTTATGTTT+TGG + chr4.2:17147005-17147024 MS.gene028280:intron 15.0%
!!! TATATACTGATATTGATTTT+TGG - chr4.2:17148309-17148328 None:intergenic 15.0%
!!! TTATTAGAATTAAGCAATTT+TGG + chr4.2:17148354-17148373 MS.gene028280:intron 15.0%
!!! TTATTTTATTTTCTTAAGGA+AGG - chr4.2:17147824-17147843 None:intergenic 15.0%
!!! TTTTGGTTATTAATTAACAT+AGG + chr4.2:17148371-17148390 MS.gene028280:intron 15.0%
!! AAGTTTGGTTAATGTTATAT+TGG - chr4.2:17147205-17147224 None:intergenic 20.0%
!! ACAAAATTATAGTACAAATG+AGG - chr4.2:17148087-17148106 None:intergenic 20.0%
!! CAAAAAATAAATAGAGTGAT+AGG - chr4.2:17147535-17147554 None:intergenic 20.0%
!! CAAAATTATAGTACAAATGA+GGG - chr4.2:17148086-17148105 None:intergenic 20.0%
!! CATTAACAAACTCTATTTAA+TGG - chr4.2:17147237-17147256 None:intergenic 20.0%
!! CTAAAAATAAAAACTAGAGT+TGG - chr4.2:17147162-17147181 None:intergenic 20.0%
!! TAGAAATAGAATACTATGTT+AGG + chr4.2:17147999-17148018 MS.gene028280:intron 20.0%
!! TATTTGCAAATTAATTCATG+TGG + chr4.2:17146131-17146150 MS.gene028280:intron 20.0%
!! TGAAGTAAGTATTTAGAAAT+AGG - chr4.2:17146444-17146463 None:intergenic 20.0%
!! TTTGTGATGATATGTAAATA+TGG + chr4.2:17148031-17148050 MS.gene028280:intron 20.0%
!!! AAATAATATATGAGTTGGTT+TGG + chr4.2:17148397-17148416 MS.gene028280:intron 20.0%
!!! ATCAACTCCAAAATTTTAAA+GGG + chr4.2:17146108-17146127 MS.gene028280:intron 20.0%
!!! CAACTCTAGTTTTTATTTTT+AGG + chr4.2:17147160-17147179 MS.gene028280:intron 20.0%
!!! TATCAACTCCAAAATTTTAA+AGG + chr4.2:17146107-17146126 MS.gene028280:intron 20.0%
!!! TATTACTTCTTTTTTTCCTT+TGG + chr4.2:17146174-17146193 MS.gene028280:intron 20.0%
!!! TCACTCTATTTATTTTTTGA+AGG + chr4.2:17147536-17147555 MS.gene028280:intron 20.0%
!!! TTAAAATTTTGGAGTTGATA+AGG - chr4.2:17146107-17146126 None:intergenic 20.0%
!!! TTTGTTTGAATTTTATACGA+AGG - chr4.2:17146635-17146654 None:intergenic 20.0%
! AAAACCAATCATAGACAATT+TGG - chr4.2:17146393-17146412 None:intergenic 25.0%
! AAGTGATATTCTTAAATGAG+AGG + chr4.2:17148453-17148472 MS.gene028280:CDS 25.0%
! ACAGAAAAATAAAGGAAATC+TGG - chr4.2:17146663-17146682 None:intergenic 25.0%
! AGTGATATTCTTAAATGAGA+GGG + chr4.2:17148454-17148473 MS.gene028280:CDS 25.0%
! ATAATGTGTGACAATAATTG+AGG + chr4.2:17148136-17148155 MS.gene028280:intron 25.0%
! CAATTATTGTCACACATTAT+TGG - chr4.2:17148136-17148155 None:intergenic 25.0%
! GAAATTAATCATACAAGACA+TGG - chr4.2:17146992-17147011 None:intergenic 25.0%
! GGTAGAAATAATATATGAGT+TGG + chr4.2:17148392-17148411 MS.gene028280:intron 25.0%
! TAATGGGATAAATCAAAGTT+TGG - chr4.2:17147220-17147239 None:intergenic 25.0%
! TAGTAACTTAACTTCTGTTA+TGG + chr4.2:17146080-17146099 MS.gene028280:intron 25.0%
! TATTTGTTTGACCTGAATAT+AGG - chr4.2:17148254-17148273 None:intergenic 25.0%
! TGGTTAATGTTATATTGGAT+TGG - chr4.2:17147200-17147219 None:intergenic 25.0%
! TTTCATTTGGAGATGATTAA+AGG - chr4.2:17147363-17147382 None:intergenic 25.0%
!!! AGATTTCCTTTATTTTTCTG+TGG + chr4.2:17146662-17146681 MS.gene028280:intron 25.0%
!!! GATTTCCTTTATTTTTCTGT+GGG + chr4.2:17146663-17146682 MS.gene028280:intron 25.0%
!!! GCAAATACCCTTTAAAATTT+TGG - chr4.2:17146118-17146137 None:intergenic 25.0%
!!! TATTATTAAATTTGGTTCGG+TGG - chr4.2:17147862-17147881 None:intergenic 25.0%
AAAAAGGGATCTTCCATTAA+TGG - chr4.2:17147039-17147058 None:intergenic 30.0%
AAAAGGGATCTTCCATTAAT+GGG - chr4.2:17147038-17147057 None:intergenic 30.0%
AAAGGAAAAGAAACATCCAA+AGG - chr4.2:17146193-17146212 None:intergenic 30.0%
AATGAAATGTGACAGTTTCT+GGG - chr4.2:17147910-17147929 None:intergenic 30.0%
AGAAAGACAGAAGAAGAATT+TGG - chr4.2:17147113-17147132 None:intergenic 30.0%
AGGGGAAATTGGATAAAAAA+AGG - chr4.2:17147055-17147074 None:intergenic 30.0%
CAAACACAATGAGACAAAAT+TGG - chr4.2:17146901-17146920 None:intergenic 30.0%
GAAAGACAGAAGAAGAATTT+GGG - chr4.2:17147112-17147131 None:intergenic 30.0%
GGGGAAATTGGATAAAAAAA+GGG - chr4.2:17147054-17147073 None:intergenic 30.0%
GTTTGTCTCAAATTAGAAGT+CGG + chr4.2:17147464-17147483 MS.gene028280:intron 30.0%
TTGCTATCCTTTACAAAATC+TGG - chr4.2:17146494-17146513 None:intergenic 30.0%
TTTGCCAAATTGTCTATGAT+TGG + chr4.2:17146386-17146405 MS.gene028280:intron 30.0%
! AAACTTTTATAACCTGGTAC+AGG - chr4.2:17147448-17147467 None:intergenic 30.0%
! ACATGGATTTATGCTTTGTT+TGG + chr4.2:17146416-17146435 MS.gene028280:intron 30.0%
! TGAGACAAACTTTTATAACC+TGG - chr4.2:17147454-17147473 None:intergenic 30.0%
! TTCTACTCCAGATTTTGTAA+AGG + chr4.2:17146484-17146503 MS.gene028280:intron 30.0%
! TTTGGTTTCTTTCCCATTAA+TGG + chr4.2:17147023-17147042 MS.gene028280:intron 30.0%
!! ATATATGAGTTGGTTTGGAA+TGG + chr4.2:17148402-17148421 MS.gene028280:intron 30.0%
!!! CTATGATTGGTTTTATGACA+TGG + chr4.2:17146399-17146418 MS.gene028280:intron 30.0%
!!! TGGTTTTCTAGAATTGACAA+GGG + chr4.2:17146834-17146853 MS.gene028280:intron 30.0%
!!! TTGGTTTTCTAGAATTGACA+AGG + chr4.2:17146833-17146852 MS.gene028280:intron 30.0%
!!! TTTTCCACTTTTTGTTTTCC+AGG + chr4.2:17146958-17146977 MS.gene028280:intron 30.0%
AAAAACTAGAGTTGGGAAGT+AGG - chr4.2:17147154-17147173 None:intergenic 35.0%
AAAACTAGAGTTGGGAAGTA+GGG - chr4.2:17147153-17147172 None:intergenic 35.0%
AAGCATCAACAGTGATATCT+GGG - chr4.2:17146930-17146949 None:intergenic 35.0%
AAGCTTTCGCTTTCAATGAA+AGG - chr4.2:17147086-17147105 None:intergenic 35.0%
ATGCAGCCATATACGATTAA+CGG + chr4.2:17148425-17148444 MS.gene028280:intron 35.0%
CATTTGGAGATGATTAAAGG+TGG - chr4.2:17147360-17147379 None:intergenic 35.0%
CGAATCCCACAGAAAAATAA+AGG - chr4.2:17146671-17146690 None:intergenic 35.0%
GAATGAAATGTGACAGTTTC+TGG - chr4.2:17147911-17147930 None:intergenic 35.0%
GTTAAGTTACTAGTACCTGA+AGG - chr4.2:17146072-17146091 None:intergenic 35.0%
TAAGCATCAACAGTGATATC+TGG - chr4.2:17146931-17146950 None:intergenic 35.0%
! CTGTCTTTCTACTTCAAGTT+TGG + chr4.2:17147123-17147142 MS.gene028280:intron 35.0%
! TAATGGGTGAGACATTCTTT+GGG + chr4.2:17145936-17145955 MS.gene028280:CDS 35.0%
! TGTTCTTTTCGTTCTTCCTT+TGG - chr4.2:17145982-17146001 None:intergenic 35.0%
! TTAATGGGTGAGACATTCTT+TGG + chr4.2:17145935-17145954 MS.gene028280:CDS 35.0%
AAGGTATGTTTACCATGACG+TGG + chr4.2:17148192-17148211 MS.gene028280:CDS 40.0%
AAGTGAATCAGAAACCTGCA+TGG + chr4.2:17145903-17145922 MS.gene028280:CDS 40.0%
AAGTTACTAGTACCTGAAGG+AGG - chr4.2:17146069-17146088 None:intergenic 40.0%
AATTGGTCACTAGTGTCTAG+TGG - chr4.2:17146884-17146903 None:intergenic 40.0%
AGTTACTAGTACCTGAAGGA+GGG - chr4.2:17146068-17146087 None:intergenic 40.0%
ATTAACCTCTCTAACCATGC+AGG - chr4.2:17145920-17145939 None:intergenic 40.0%
GAGAGAGAACAGAAATGGAA+GGG - chr4.2:17148560-17148579 None:intergenic 40.0%
GCATGGTTAGAGAGGTTAAT+GGG + chr4.2:17145920-17145939 MS.gene028280:CDS 40.0%
TACCATGACGTGGTTCAATT+GGG + chr4.2:17148202-17148221 MS.gene028280:CDS 40.0%
TCAATGAAAGGAACGTTGCA+GGG - chr4.2:17147074-17147093 None:intergenic 40.0%
TGAGACAACAGTGCAAACAA+AGG - chr4.2:17146006-17146025 None:intergenic 40.0%
TGAGAGAGAACAGAAATGGA+AGG - chr4.2:17148561-17148580 None:intergenic 40.0%
TGCATGAGAGAGAACAGAAA+TGG - chr4.2:17148565-17148584 None:intergenic 40.0%
TGCATGGTTAGAGAGGTTAA+TGG + chr4.2:17145919-17145938 MS.gene028280:CDS 40.0%
TTACCATGACGTGGTTCAAT+TGG + chr4.2:17148201-17148220 MS.gene028280:CDS 40.0%
TTCAATGAAAGGAACGTTGC+AGG - chr4.2:17147075-17147094 None:intergenic 40.0%
TTCGCACCGTTAATCGTATA+TGG - chr4.2:17148434-17148453 None:intergenic 40.0%
TTGGAGATGATTAAAGGTGG+AGG - chr4.2:17147357-17147376 None:intergenic 40.0%
TTTCTGTTCTCTCTCATGCA+AGG + chr4.2:17148565-17148584 MS.gene028280:CDS 40.0%
! AAATGAGGGTATGAGTAAGC+AGG - chr4.2:17148072-17148091 None:intergenic 40.0%
! AAGGAGTGTGAGAGTTAAAG+AGG - chr4.2:17147805-17147824 None:intergenic 40.0%
! AGGAGTGTGAGAGTTAAAGA+GGG - chr4.2:17147804-17147823 None:intergenic 40.0%
! CATCGATTGCTCCTATATTC+AGG + chr4.2:17148240-17148259 MS.gene028280:CDS 40.0%
! CTTTTATAACCTGGTACAGG+AGG - chr4.2:17147445-17147464 None:intergenic 40.0%
! TTTCGTTCTTCCTTTGGTTC+TGG - chr4.2:17145976-17145995 None:intergenic 40.0%
!! TTCTAGAATTGACAAGGGCT+TGG + chr4.2:17146839-17146858 MS.gene028280:intron 40.0%
!!! CCACTTTTTGTTTTCCAGGA+GGG + chr4.2:17146962-17146981 MS.gene028280:intron 40.0%
!!! TCCACTTTTTGTTTTCCAGG+AGG + chr4.2:17146961-17146980 MS.gene028280:intron 40.0%
ACCTTCTCACCTGCACAATA+TGG - chr4.2:17148177-17148196 None:intergenic 45.0%
AGAAACCTGCATGGTTAGAG+AGG + chr4.2:17145912-17145931 MS.gene028280:CDS 45.0%
CAATGAAAGGAACGTTGCAG+GGG - chr4.2:17147073-17147092 None:intergenic 45.0%
CACTTGTGACAGGATTCTTC+AGG + chr4.2:17148532-17148551 MS.gene028280:CDS 45.0%
CCCTCCTGGAAAACAAAAAG+TGG - chr4.2:17146965-17146984 None:intergenic 45.0%
CTATTGCTGCCATATTGTGC+AGG + chr4.2:17148165-17148184 MS.gene028280:intron 45.0%
CTCCCTTTGCTTCTGATGAT+GGG - chr4.2:17145877-17145896 None:intergenic 45.0%
GACCCATCATCAGAAGCAAA+GGG + chr4.2:17145872-17145891 MS.gene028280:CDS 45.0%
GATGAGAAGAGAGACAGTGA+GGG - chr4.2:17146036-17146055 None:intergenic 45.0%
GTTACTAGTACCTGAAGGAG+GGG - chr4.2:17146067-17146086 None:intergenic 45.0%
TCTCCCTTTGCTTCTGATGA+TGG - chr4.2:17145878-17145897 None:intergenic 45.0%
TGACCCATCATCAGAAGCAA+AGG + chr4.2:17145871-17145890 MS.gene028280:CDS 45.0%
! AGACATTCTTTGGGCGTTGT+GGG + chr4.2:17145945-17145964 MS.gene028280:CDS 45.0%
! TAAATGAGAGGGTGCAGTCA+AGG + chr4.2:17148465-17148484 MS.gene028280:CDS 45.0%
!! TTTATAATATTACATAAATA+TGG - chr4.2:17147272-17147291 None:intergenic 5.0%
!!! AAAATAAATATTTTTGTTAA+GGG + chr4.2:17147721-17147740 MS.gene028280:intron 5.0%
!!! AATTAATTTAAATTTTCATT+TGG - chr4.2:17147376-17147395 None:intergenic 5.0%
!!! ATTTTATTTTTATTTTTTTC+GGG + chr4.2:17147327-17147346 MS.gene028280:intron 5.0%
ACTCTTGCTGCACTTGTGAC+AGG + chr4.2:17148522-17148541 MS.gene028280:CDS 50.0%
AGAGAGACAGTGAGGGCAAA+TGG - chr4.2:17146029-17146048 None:intergenic 50.0%
AGGAACGTTGCAGGGGAAAT+TGG - chr4.2:17147066-17147085 None:intergenic 50.0%
AGTGCAGCAAGAGTTGGCAT+TGG - chr4.2:17148516-17148535 None:intergenic 50.0%
CACCCAATTGAACCACGTCA+TGG - chr4.2:17148207-17148226 None:intergenic 50.0%
GCCATATTGTGCAGGTGAGA+AGG + chr4.2:17148173-17148192 MS.gene028280:intron 50.0%
GGATGAGAAGAGAGACAGTG+AGG - chr4.2:17146037-17146056 None:intergenic 50.0%
GTCACAAGTGCAGCAAGAGT+TGG - chr4.2:17148522-17148541 None:intergenic 50.0%
GTGCAGTCAAGGTCATGCAA+AGG + chr4.2:17148476-17148495 MS.gene028280:CDS 50.0%
TCACTAGTGTCTAGTGGTGG+TGG - chr4.2:17146878-17146897 None:intergenic 50.0%
TGGTCACTAGTGTCTAGTGG+TGG - chr4.2:17146881-17146900 None:intergenic 50.0%
! GACATTCTTTGGGCGTTGTG+GGG + chr4.2:17145946-17145965 MS.gene028280:CDS 50.0%
! GAGACATTCTTTGGGCGTTG+TGG + chr4.2:17145944-17145963 MS.gene028280:CDS 50.0%
!! CGTTCTTCCTTTGGTTCTGG+TGG - chr4.2:17145973-17145992 None:intergenic 50.0%
ACCTGAAGGAGGGGATGAGA+GGG - chr4.2:17146058-17146077 None:intergenic 55.0%
CAAGACATGGTGAACCCTCC+TGG - chr4.2:17146979-17146998 None:intergenic 55.0%
TACCTGAAGGAGGGGATGAG+AGG - chr4.2:17146059-17146078 None:intergenic 55.0%
TCTTGCACTCCTCCTGTACC+AGG + chr4.2:17147433-17147452 MS.gene028280:intron 55.0%
GTGGGGTCCACCAGAACCAA+AGG + chr4.2:17145963-17145982 MS.gene028280:CDS 60.0%
TCCCTCTCATCCCCTCCTTC+AGG + chr4.2:17146054-17146073 MS.gene028280:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 17145854 17148601 17145854 ID=MS.gene028280
chr4.2 mRNA 17145854 17148601 17145854 ID=MS.gene028280.t1;Parent=MS.gene028280
chr4.2 exon 17145854 17146075 17145854 ID=MS.gene028280.t1.exon1;Parent=MS.gene028280.t1
chr4.2 CDS 17145854 17146075 17145854 ID=cds.MS.gene028280.t1;Parent=MS.gene028280.t1
chr4.2 exon 17148187 17148261 17148187 ID=MS.gene028280.t1.exon2;Parent=MS.gene028280.t1
chr4.2 CDS 17148187 17148261 17148187 ID=cds.MS.gene028280.t1;Parent=MS.gene028280.t1
chr4.2 exon 17148431 17148601 17148431 ID=MS.gene028280.t1.exon3;Parent=MS.gene028280.t1
chr4.2 CDS 17148431 17148601 17148431 ID=cds.MS.gene028280.t1;Parent=MS.gene028280.t1
Gene Sequence

>MS.gene028280

ATGAGAGGAAGTGGAAGTGACCCATCATCAGAAGCAAAGGGAGACATGAAAGTGAATCAGAAACCTGCATGGTTAGAGAGGTTAATGGGTGAGACATTCTTTGGGCGTTGTGGGGTCCACCAGAACCAAAGGAAGAACGAAAAGAACATCCTTTGTTTGCACTGTTGTCTCACCATTTGCCCTCACTGTCTCTCTTCTCATCCCTCTCATCCCCTCCTTCAGGTACTAGTAACTTAACTTCTGTTATGGACCTTATCAACTCCAAAATTTTAAAGGGTATTTGCAAATTAATTCATGTGGTTATAGTCTTCCTTAATTTCTATTACTTCTTTTTTTCCTTTGGATGTTTCTTTTCCTTTAGATTACTTTATTCTATTACAAATGATTAAATTCAGATGCACCTATATATATGTTCTTCAATTAATGAAGTTTTTTGAACAATTTTAAAAGTTTTTTTTCAATGTGAAAAATTGTTGATGATGATTTTAGTAGCTGAACTTCTTATTTTTGATAAATTTGATAGGTGATATTATTTGCCAAATTGTCTATGATTGGTTTTATGACATGGATTTATGCTTTGTTTGGTGCCTATTTCTAAATACTTACTTCAAATTCTTTTTCTCGAAATCTTTCTACTCCAGATTTTGTAAAGGATAGCAATTAAGTTTAATTTTGTAAAGGATAATTGAGTCAAGAATTTTTTATTTAAACATTTGGAAATTTGCAACGTTATCGAAACATAACATAATTGTTTTTTTAATTAAAAAACACTATATATCCTTCGTATAAAATTCAAACAAATCACACCAGATTTCCTTTATTTTTCTGTGGGATTCGAACTAACATAATTTGTGTTTAAAAAAAAAACAGCTTAGAAATTATGTTTCTACTTGATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATAGGATTAGTTCTATTTTCTTTTAATTTAATTTTTATTTTTTGGTTTTCTAGAATTGACAAGGGCTTGGTAAATTGAACTCACCACCACCACTAGACACTAGTGACCAATTTTGTCTCATTGTGTTTGATGTTTCCCAGATATCACTGTTGATGCTTAAACACAGTTTTCCACTTTTTGTTTTCCAGGAGGGTTCACCATGTCTTGTATGATTAATTTCTACTTTTATGTTTTGGTTTCTTTCCCATTAATGGAAGATCCCTTTTTTTATCCAATTTCCCCTGCAACGTTCCTTTCATTGAAAGCGAAAGCTTTGACCCAAATTCTTCTTCTGTCTTTCTACTTCAAGTTTGGAAAGCCCTACTTCCCAACTCTAGTTTTTATTTTTAGGACATGTGCATAGTTCCAATCCAATATAACATTAACCAAACTTTGATTTATCCCATTAAATAGAGTTTGTTAATGAATTAAACTACGCCATATTTATGTAATATTATAAAATTATATTTGAATTCAACTCATTCATCTTTATTTTATTTTATTTTTATTTTTTTCGGGTTTTCCTCCACCTTTAATCATCTCCAAATGAAAATTTAAATTAATTCAATGATTATTGTAATAAAGAATAATTTAATGGTTACTCTTGCACTCCTCCTGTACCAGGTTATAAAAGTTTGTCTCAAATTAGAAGTCGGTTTACAAAATATAATTGATCTAACAATGAAAAATTACTGTACTTTCCTATCACTCTATTTATTTTTTGAAGGACATCACTCTATTTATTATTTAGTATTTTTCTTTGAATTTTATTACTATTTCTTTTTCTCATGCTATTATTAATGAAAAATAATTTTGTAAAACAACTTATAGGTTTCCGTATAAGATTAACTATATTTAAGTTGAATAAAAGTATCAAAATGATCTTGACCAAAATAAATATTTTTGTTAAGGGAATGATTGAATTCTGAACACAATTATCAATATTTCACTCTTTAACATTAAAAAAAAACCCTCTTTAACTCTCACACTCCTTCCTTAAGAAAATAAAATAAAATCTCACACTCTCACCACCGAACCAAATTTAATAATATTTCATTGGATGACATTATTTTCATCCCAGAAACTGTCACATTTCATTCATATTTTTAGTTTGAATTCTTCATGTATAACTTAAAACTTAAACTCAAAAGTATTTTAATGTATGTGATAGAAATAGAATACTATGTTAGGTAAACAAATTTTGTGATGATATGTAAATATGGAAAGTTGTTTTTTTGCCTGCTTACTCATACCCTCATTTGTACTATAATTTTGTATTGTATTTTGTATTTGTATGAATGACCAATAATGTGTGACAATAATTGAGGTGTTTGCTATTGCTGCCATATTGTGCAGGTGAGAAGGTATGTTTACCATGACGTGGTTCAATTGGGTGATCTTGAAAAGCTCATCGATTGCTCCTATATTCAGGTCAAACAAATACTACTACTCTAAAAATAAAATATTTCACTAACCCAAAAATCAATATCAGTATATATTACGTGAAAAATAATGATAATTAATTATTAGAATTAAGCAATTTTGGTTATTAATTAACATAGGTAGAAATAATATATGAGTTGGTTTGGAATGGATGCAGCCATATACGATTAACGGTGCGAAAGTGATATTCTTAAATGAGAGGGTGCAGTCAAGGTCATGCAAAGGTACTACTACTGCTGCCAATGCCAACTCTTGCTGCACTTGTGACAGGATTCTTCAGGATCCCTTCCATTTCTGTTCTCTCTCATGCAAGGTAATGTATCCCTAA
Protein sequence

>MS.gene028280.t1

MRGSGSDPSSEAKGDMKVNQKPAWLERLMGETFFGRCGVHQNQRKNEKNILCLHCCLTICPHCLSSHPSHPLLQVRRYVYHDVVQLGDLEKLIDCSYIQPYTINGAKVIFLNERVQSRSCKGTTTAANANSCCTCDRILQDPFHFCSLSCKVMYP