AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028311

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028311.t1 MTR_0402s0040 71.905 210 58 1 12 221 2 210 1.57e-95 303
MS.gene028311.t1 MTR_0402s0040 60.331 242 57 2 397 638 248 450 3.55e-78 257
MS.gene028311.t1 MTR_7g113010 67.702 161 36 2 1 161 424 568 3.38e-57 203
MS.gene028311.t1 MTR_6g017200 72.519 131 35 1 2 131 23 153 1.49e-56 189
MS.gene028311.t1 MTR_1g057920 76.800 125 27 1 1 125 1 123 4.96e-56 186
MS.gene028311.t1 MTR_5g015480 61.212 165 60 1 1 165 25 185 4.89e-54 186
MS.gene028311.t1 MTR_2g070590 70.833 120 35 0 20 139 1 120 8.21e-51 172
MS.gene028311.t1 MTR_6g051425 32.248 307 179 8 35 317 13 314 4.69e-46 167
MS.gene028311.t1 MTR_0558s0020 33.463 257 152 4 40 277 40 296 5.82e-43 157
MS.gene028311.t1 MTR_8g446020 37.714 175 108 1 104 277 82 256 4.01e-35 134
MS.gene028311.t1 MTR_6g090505 35.071 211 122 3 81 276 20 230 1.52e-34 135
MS.gene028311.t1 MTR_0137s0040 57.692 104 44 0 1 104 1 104 8.40e-32 119
MS.gene028311.t1 MTR_1g070355 32.627 236 147 5 42 271 80 309 7.54e-31 127
MS.gene028311.t1 MTR_1g083640 29.825 228 141 4 40 248 55 282 5.62e-29 117
MS.gene028311.t1 MTR_4g035820 31.560 282 177 9 9 281 50 324 1.56e-27 118
MS.gene028311.t1 MTR_7g074790 41.143 175 81 4 33 206 7 160 2.15e-26 108
MS.gene028311.t1 MTR_1g050985 78.333 60 13 0 106 165 39 98 1.03e-25 102
MS.gene028311.t1 MTR_4g102720 33.140 172 79 3 33 204 4 139 2.45e-23 99.4
MS.gene028311.t1 MTR_0291s0060 64.865 74 26 0 13 86 12 85 2.75e-23 94.7
MS.gene028311.t1 MTR_5g039760 34.804 204 103 6 32 232 6 182 1.23e-21 93.6
MS.gene028311.t1 MTR_7g446520 30.579 242 150 4 42 282 94 318 1.25e-21 97.1
MS.gene028311.t1 MTR_7g061980 42.017 119 65 3 30 147 4 119 1.57e-20 88.2
MS.gene028311.t1 MTR_7g446480 35.795 176 110 2 112 285 140 314 1.88e-20 93.2
MS.gene028311.t1 MTR_0337s0040 29.480 173 109 3 82 242 51 222 4.22e-20 90.1
MS.gene028311.t1 MTR_7g446510 35.227 176 111 2 112 285 155 329 6.03e-20 92.0
MS.gene028311.t1 MTR_5g076070 26.872 227 160 4 42 265 59 282 1.21e-18 87.0
MS.gene028311.t1 MTR_1g008290 34.965 143 88 3 89 230 3 141 5.26e-17 79.0
MS.gene028311.t1 MTR_2g014100 25.126 199 129 3 40 219 48 245 9.05e-17 80.9
MS.gene028311.t1 MTR_1g019850 33.742 163 101 2 7 169 41 196 9.25e-17 80.5
MS.gene028311.t1 MTR_1g086270 56.061 66 21 2 331 396 1 58 6.77e-16 74.3
MS.gene028311.t1 MTR_4g067290 41.667 108 57 3 35 140 55 158 8.32e-16 77.8
MS.gene028311.t1 MTR_4g067290 41.667 108 57 3 35 140 55 158 8.63e-16 77.8
MS.gene028311.t1 MTR_4g067290 41.667 108 57 3 35 140 75 178 1.34e-15 77.8
MS.gene028311.t1 MTR_4g067290 41.667 108 57 3 35 140 73 176 1.37e-15 77.4
MS.gene028311.t1 MTR_1g091013 53.846 78 25 1 27 104 3 69 1.43e-15 72.0
MS.gene028311.t1 MTR_4g067290 41.667 108 57 3 35 140 75 178 1.43e-15 77.4
MS.gene028311.t1 MTR_4g067290 41.667 108 57 3 35 140 115 218 1.83e-15 77.8
MS.gene028311.t1 MTR_2g098920 38.889 108 60 3 35 140 79 182 6.44e-15 75.9
MS.gene028311.t1 MTR_5g045210 39.326 89 54 0 488 576 20 108 7.23e-14 71.2
MS.gene028311.t1 MTR_3g006370 38.095 105 63 2 35 138 49 152 2.55e-13 70.5
MS.gene028311.t1 MTR_1g055240 31.624 117 80 0 104 220 33 149 8.10e-13 67.0
MS.gene028311.t1 MTR_3g450850 24.194 248 174 6 41 276 52 297 2.54e-12 69.3
MS.gene028311.t1 MTR_2g099430 37.079 89 54 1 59 147 26 112 6.09e-12 63.2
MS.gene028311.t1 MTR_6g022080 26.582 158 113 2 42 197 91 247 6.57e-12 66.6
MS.gene028311.t1 MTR_3g008240 25.000 164 105 3 39 184 39 202 2.93e-11 63.5
MS.gene028311.t1 MTR_2g098880 33.071 127 78 4 22 142 44 169 3.35e-11 64.3
MS.gene028311.t1 MTR_2g098880 33.071 127 78 4 22 142 56 181 3.55e-11 64.7
MS.gene028311.t1 MTR_0019s0290 29.464 112 79 0 104 215 48 159 3.85e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028311.t1 AT4G38180 37.681 345 209 4 23 363 60 402 2.63e-75 257
MS.gene028311.t1 AT4G38180 30.049 203 136 4 380 577 499 700 4.79e-15 79.3
MS.gene028311.t1 AT4G15090 33.735 332 216 4 33 362 48 377 1.16e-58 212
MS.gene028311.t1 AT4G15090 26.639 244 150 6 389 622 484 708 6.81e-14 75.9
MS.gene028311.t1 AT4G15090 33.735 332 216 4 33 362 48 377 1.16e-58 212
MS.gene028311.t1 AT4G15090 26.639 244 150 6 389 622 484 708 6.81e-14 75.9
MS.gene028311.t1 AT4G15090 33.735 332 216 4 33 362 48 377 1.16e-58 212
MS.gene028311.t1 AT4G15090 26.639 244 150 6 389 622 484 708 6.81e-14 75.9
MS.gene028311.t1 AT4G15090 33.735 332 216 4 33 362 48 377 1.16e-58 212
MS.gene028311.t1 AT4G15090 26.639 244 150 6 389 622 484 708 6.81e-14 75.9
MS.gene028311.t1 AT4G15090 33.735 332 216 4 33 362 48 377 1.16e-58 212
MS.gene028311.t1 AT4G15090 26.639 244 150 6 389 622 484 708 6.81e-14 75.9
MS.gene028311.t1 AT1G76320 33.333 324 203 6 40 361 1 313 4.92e-52 191
MS.gene028311.t1 AT1G76320 33.333 174 100 7 389 556 421 584 1.50e-14 77.8
MS.gene028311.t1 AT1G76320 33.333 324 203 6 40 361 1 313 1.43e-51 190
MS.gene028311.t1 AT1G76320 32.948 173 102 6 389 556 421 584 1.03e-14 78.2
MS.gene028311.t1 AT1G76320 33.333 324 203 6 40 361 1 313 1.43e-51 190
MS.gene028311.t1 AT1G76320 32.948 173 102 6 389 556 421 584 1.03e-14 78.2
MS.gene028311.t1 AT1G76320 33.333 324 203 6 40 361 1 313 1.60e-51 190
MS.gene028311.t1 AT1G76320 32.948 173 102 6 389 556 421 584 1.07e-14 78.2
MS.gene028311.t1 AT1G10240 33.434 332 208 9 29 351 41 368 2.46e-51 189
MS.gene028311.t1 AT1G10240 33.434 332 208 9 29 351 41 368 2.46e-51 189
MS.gene028311.t1 AT3G22170 29.971 347 205 6 33 365 67 389 2.77e-46 176
MS.gene028311.t1 AT3G22170 34.270 178 94 6 389 556 493 657 3.34e-18 89.7
MS.gene028311.t1 AT3G22170 29.971 347 205 6 33 365 67 389 2.77e-46 176
MS.gene028311.t1 AT3G22170 34.270 178 94 6 389 556 493 657 3.34e-18 89.7
MS.gene028311.t1 AT5G28530 23.891 586 353 16 36 531 55 637 5.00e-43 165
MS.gene028311.t1 AT5G28530 23.891 586 353 16 36 531 55 637 5.00e-43 165
MS.gene028311.t1 AT3G06250 30.275 327 187 6 27 352 181 467 7.93e-42 162
MS.gene028311.t1 AT3G06250 29.282 181 119 5 393 568 582 758 2.06e-13 74.3
MS.gene028311.t1 AT3G06250 30.275 327 187 6 27 352 181 467 7.93e-42 162
MS.gene028311.t1 AT3G06250 29.282 181 119 5 393 568 582 758 2.06e-13 74.3
MS.gene028311.t1 AT3G06250 30.275 327 187 6 27 352 181 467 7.93e-42 162
MS.gene028311.t1 AT3G06250 29.282 181 119 5 393 568 582 758 2.06e-13 74.3
MS.gene028311.t1 AT2G27110 31.250 352 206 9 28 373 41 362 2.70e-41 161
MS.gene028311.t1 AT2G27110 34.184 196 119 6 380 569 444 635 3.76e-20 95.9
MS.gene028311.t1 AT2G27110 31.250 352 206 9 28 373 41 362 2.70e-41 161
MS.gene028311.t1 AT2G27110 34.184 196 119 6 380 569 444 635 3.76e-20 95.9
MS.gene028311.t1 AT2G32250 28.916 332 187 8 37 365 39 324 1.65e-39 155
MS.gene028311.t1 AT2G32250 26.778 239 158 7 339 568 375 605 2.64e-13 73.9
MS.gene028311.t1 AT5G28530 27.446 368 239 9 36 383 55 414 1.87e-39 155
MS.gene028311.t1 AT5G28530 29.252 147 99 2 390 531 495 641 1.23e-11 68.2
MS.gene028311.t1 AT2G32250 28.916 332 187 8 37 365 39 324 2.60e-39 155
MS.gene028311.t1 AT2G32250 26.778 239 158 7 339 568 375 605 2.46e-13 73.9
MS.gene028311.t1 AT2G32250 28.916 332 187 8 37 365 39 324 2.60e-39 155
MS.gene028311.t1 AT2G32250 26.778 239 158 7 339 568 375 605 2.46e-13 73.9
MS.gene028311.t1 AT2G32250 28.916 332 187 8 37 365 39 324 2.80e-39 155
MS.gene028311.t1 AT2G32250 26.778 239 158 7 339 568 375 605 2.32e-13 73.9
MS.gene028311.t1 AT2G32250 28.916 332 187 8 37 365 39 324 2.80e-39 155
MS.gene028311.t1 AT2G32250 26.778 239 158 7 339 568 375 605 2.32e-13 73.9
MS.gene028311.t1 AT2G32250 28.916 332 187 8 37 365 39 324 2.80e-39 155
MS.gene028311.t1 AT2G32250 26.778 239 158 7 339 568 375 605 2.32e-13 73.9
MS.gene028311.t1 AT2G32250 28.875 329 185 8 40 365 1 283 3.65e-39 154
MS.gene028311.t1 AT2G32250 26.778 239 158 7 339 568 334 564 3.26e-13 73.6
MS.gene028311.t1 AT2G32250 28.875 329 185 8 40 365 1 283 3.89e-39 154
MS.gene028311.t1 AT2G32250 26.778 239 158 7 339 568 334 564 3.21e-13 73.6
MS.gene028311.t1 AT5G18960 29.969 327 189 8 29 353 204 492 1.46e-38 152
MS.gene028311.t1 AT5G18960 30.435 161 104 4 413 568 625 782 5.42e-14 75.9
MS.gene028311.t1 AT5G18960 32.051 156 86 4 17 172 28 163 2.24e-11 67.8
MS.gene028311.t1 AT5G18960 29.969 327 189 8 29 353 204 492 1.46e-38 152
MS.gene028311.t1 AT5G18960 30.435 161 104 4 413 568 625 782 5.42e-14 75.9
MS.gene028311.t1 AT5G18960 32.051 156 86 4 17 172 28 163 2.24e-11 67.8
MS.gene028311.t1 AT1G80010 27.233 459 276 12 21 435 80 524 2.04e-36 146
MS.gene028311.t1 AT1G52520 29.664 327 195 9 36 351 85 387 1.53e-34 140
MS.gene028311.t1 AT4G19990 27.824 363 208 8 42 387 27 352 2.55e-32 134
MS.gene028311.t1 AT4G19990 32.143 168 88 4 413 566 455 610 3.51e-11 67.0
MS.gene028311.t1 AT4G19990 27.824 363 208 8 42 387 41 366 2.61e-32 134
MS.gene028311.t1 AT4G19990 32.143 168 88 4 413 566 469 624 3.46e-11 67.0
MS.gene028311.t1 AT4G19990 27.824 363 208 8 42 387 27 352 2.98e-32 133
MS.gene028311.t1 AT4G19990 32.143 168 88 4 413 566 455 610 3.42e-11 67.0
MS.gene028311.t1 AT4G19990 27.824 363 208 8 42 387 27 352 2.98e-32 133
MS.gene028311.t1 AT4G19990 32.143 168 88 4 413 566 455 610 3.42e-11 67.0
MS.gene028311.t1 AT4G19990 27.824 363 208 8 42 387 27 352 3.52e-32 133
MS.gene028311.t1 AT4G19990 31.609 174 93 4 413 572 455 616 4.06e-11 66.6
MS.gene028311.t1 AT4G19990 27.824 363 208 8 42 387 27 352 3.52e-32 133
MS.gene028311.t1 AT4G19990 31.609 174 93 4 413 572 455 616 4.06e-11 66.6
MS.gene028311.t1 AT2G27110 39.877 163 94 2 215 373 55 217 4.12e-32 132
MS.gene028311.t1 AT2G27110 34.184 196 119 6 380 569 299 490 3.00e-20 95.9
MS.gene028311.t1 AT4G19990 26.970 330 187 8 75 387 3 295 5.25e-25 110
MS.gene028311.t1 AT4G19990 32.143 168 88 4 413 566 398 553 3.36e-11 67.0
MS.gene028311.t1 AT4G38170 31.795 195 119 5 204 396 10 192 1.11e-24 108
MS.gene028311.t1 AT4G38170 32.867 143 90 3 380 517 264 405 8.11e-17 84.3
MS.gene028311.t1 AT5G28530 22.905 358 205 10 36 383 55 351 1.82e-20 96.3
MS.gene028311.t1 AT5G28530 29.252 147 99 2 390 531 432 578 1.32e-11 68.2
MS.gene028311.t1 AT5G28530 22.905 358 205 10 36 383 55 351 2.16e-20 95.9
MS.gene028311.t1 AT5G28530 29.252 147 99 2 390 531 428 574 1.26e-11 68.2
MS.gene028311.t1 AT2G43280 38.889 126 72 4 34 155 20 144 7.58e-17 80.1
MS.gene028311.t1 AT3G59470 35.915 142 79 4 9 140 33 172 5.05e-15 75.9
MS.gene028311.t1 AT3G59470 35.915 142 79 4 9 140 33 172 5.05e-15 75.9
MS.gene028311.t1 AT3G59470 36.620 142 78 4 9 140 45 184 6.46e-15 75.9
MS.gene028311.t1 AT4G12850 37.302 126 72 3 36 161 11 129 9.37e-14 69.3
MS.gene028311.t1 AT4G12850 40.385 104 58 2 36 139 11 110 6.26e-13 67.0
MS.gene028311.t1 AT2G43280 37.000 100 59 3 51 147 2 100 1.51e-12 66.6
MS.gene028311.t1 AT4G12850 39.423 104 59 2 36 139 11 110 1.60e-12 67.0
MS.gene028311.t1 AT3G07500 38.318 107 65 1 34 140 31 136 1.15e-11 65.1

Find 124 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAAATAACAAACATATTT+TGG 0.063618 4.2:+17562113 MS.gene028311:CDS
CATGAATTCTTCGTCTATTT+TGG 0.166486 4.2:+17561400 None:intergenic
AGACAATTCTGTTGATAAAT+TGG 0.199895 4.2:+17561424 MS.gene028311:CDS
GCACAAATGATCAATGATTA+TGG 0.212099 4.2:+17562142 MS.gene028311:CDS
CATTTCATGGATTGTGTACT+TGG 0.244889 4.2:+17562425 MS.gene028311:CDS
ATATAAGACAAAACGCTTTA+AGG 0.257518 4.2:+17562449 MS.gene028311:CDS
TTTGGTATTCGTCGAGAATA+TGG 0.265133 4.2:+17561581 MS.gene028311:CDS
ATGTCCTCCAATGATTAAAT+TGG 0.279912 4.2:+17563254 MS.gene028311:CDS
ATATATTCATGCAAATCTTT+TGG 0.296908 4.2:-17561911 None:intergenic
CACTGTACCGCTTCATGAAA+TGG 0.307019 4.2:-17563227 None:intergenic
ACAAGCATCACAAATTATAA+TGG 0.308529 4.2:+17561868 MS.gene028311:CDS
GCTTACTTTGTTGATATAGA+TGG 0.319643 4.2:-17562480 None:intergenic
AGAATTTGCTTCGAACAAAA+AGG 0.321129 4.2:+17561987 MS.gene028311:CDS
GTTATGCCAGAGACATTTCA+TGG 0.324231 4.2:+17562412 MS.gene028311:CDS
CGCTTTAGTATATAGTGTTC+AGG 0.332129 4.2:-17563132 None:intergenic
AATGAGTATAGTAAAAGAAT+TGG 0.336174 4.2:+17561557 MS.gene028311:CDS
CACCACATACAAGACAAATA+AGG 0.340827 4.2:+17562192 MS.gene028311:CDS
CTTGACAATGGTTTCCATGC+TGG 0.341002 4.2:-17563442 None:intergenic
ATTTCACTTGATCGAAAGAT+AGG 0.358658 4.2:+17561746 MS.gene028311:CDS
CTGTTGGCCAATTTAATCAT+TGG 0.359632 4.2:-17563261 None:intergenic
ATTATAATGGGAGATGAATC+TGG 0.362761 4.2:+17561881 MS.gene028311:CDS
CGGCCGTTGGGAGTATTTGT+TGG 0.367992 4.2:+17562220 MS.gene028311:CDS
TATAGTAAAAGAATTGGATT+TGG 0.393932 4.2:+17561563 MS.gene028311:CDS
GATATTCCTCGTATTCCTCT+TGG 0.399365 4.2:-17562918 None:intergenic
GCTGGACTCTTCATAAACTT+TGG 0.408472 4.2:-17563319 None:intergenic
AGAATCAAGTGGAATGGTTC+AGG 0.410213 4.2:+17563383 MS.gene028311:CDS
TGTAAAAGAGAGGACAGTTA+TGG 0.424574 4.2:+17562999 MS.gene028311:CDS
CAAGCATCACAAATTATAAT+GGG 0.429280 4.2:+17561869 MS.gene028311:CDS
AGGATGGACTCACTAAAGTA+TGG 0.430267 4.2:+17562007 MS.gene028311:CDS
ATCATGTGAACATACTCTTC+CGG 0.437349 4.2:-17561818 None:intergenic
TGCATAAAGGTGTTGGAAGA+AGG 0.442515 4.2:+17562949 MS.gene028311:CDS
ACAAGACAAATAAGGGTTAT+CGG 0.444271 4.2:+17562200 MS.gene028311:CDS
ACCACATACAAGACAAATAA+GGG 0.447196 4.2:+17562193 MS.gene028311:CDS
CTCTATCCCACCAACCTGCT+TGG 0.449496 4.2:-17561936 None:intergenic
GACAAAAGACGTCAACCGAC+TGG 0.454042 4.2:+17561677 MS.gene028311:CDS
GTAAAAGAGAGGACAGTTAT+GGG 0.455513 4.2:+17563000 MS.gene028311:CDS
GGTTGGGAAGTAATCAAGAT+TGG 0.457422 4.2:+17563172 MS.gene028311:CDS
TCAAATAACCACTGAAAAGA+AGG 0.457713 4.2:-17562304 None:intergenic
CACAAATGATCAATGATTAT+GGG 0.462910 4.2:+17562143 MS.gene028311:CDS
TGAAAGTCATAAAAGAAAGA+TGG 0.464163 4.2:-17562076 None:intergenic
GAAACCATTGTCAAGGCAAA+AGG 0.466284 4.2:+17563450 MS.gene028311:CDS
ACTGTACCGCTTCATGAAAT+GGG 0.469454 4.2:-17563226 None:intergenic
AGCATCTTGATCCGTTAGAA+TGG 0.469910 4.2:-17562365 None:intergenic
TAAATTGGCCAACAGAGCTT+CGG 0.473711 4.2:+17563269 MS.gene028311:CDS
TGCCGACATCAAACCTCGCT+TGG 0.474766 4.2:+17561493 MS.gene028311:CDS
CACAATCCATGAAATGTCTC+TGG 0.475367 4.2:-17562418 None:intergenic
AATGGGCATGGACTTATGTT+CGG 0.478812 4.2:+17562641 MS.gene028311:CDS
ATATCAAGACACTTGCATAA+AGG 0.478993 4.2:+17562936 MS.gene028311:CDS
ATCAATGATTATGGGTACTT+TGG 0.481641 4.2:+17562151 MS.gene028311:CDS
GGGTGTAAACATTTCCGGCT+TGG 0.486090 4.2:-17562876 None:intergenic
ACCCTTATTTGTCTTGTATG+TGG 0.486140 4.2:-17562194 None:intergenic
AATAAGGGTTATCGGCCGTT+GGG 0.489965 4.2:+17562208 MS.gene028311:CDS
AACCGGGTGTAATGCACGTA+TGG 0.493902 4.2:+17561721 MS.gene028311:CDS
TTTATCATTTCCAAGTGATA+CGG 0.494676 4.2:-17563409 None:intergenic
TTGGTATTCGTCGAGAATAT+GGG 0.496079 4.2:+17561582 MS.gene028311:CDS
TGGACAAGAGAGGCAAGGTT+GGG 0.506606 4.2:+17563156 MS.gene028311:CDS
TCTACGGCAGAAACTAGAAC+CGG 0.508196 4.2:+17561704 MS.gene028311:CDS
CAAAATAGTTGAAGATATGT+TGG 0.512003 4.2:+17563341 MS.gene028311:CDS
AGACACTTGCATAAAGGTGT+TGG 0.514270 4.2:+17562942 MS.gene028311:CDS
CATACTCTTCCGGCTGAAGA+AGG 0.514601 4.2:-17561808 None:intergenic
TTGGCCAATTTAATCATTGG+AGG 0.515834 4.2:-17563258 None:intergenic
TTGTTGGACTTAATAATCAT+CGG 0.518157 4.2:+17562236 MS.gene028311:CDS
ACCTAGATTGAAAATGAAGA+AGG 0.518870 4.2:+17562840 MS.gene028311:CDS
ATGGACAAGAGAGGCAAGGT+TGG 0.520495 4.2:+17563155 MS.gene028311:CDS
GTTGGTGGGATAGAGATGGT+TGG 0.521078 4.2:+17561944 MS.gene028311:CDS
ATGAGATTCCGAAGCTCTGT+TGG 0.521741 4.2:-17563277 None:intergenic
AAATAAGGGTTATCGGCCGT+TGG 0.523633 4.2:+17562207 MS.gene028311:CDS
CAAGATGCTGCGATGGCAAA+GGG 0.528327 4.2:+17562379 MS.gene028311:CDS
GAAGTAATCAAGATTGGAAA+GGG 0.535116 4.2:+17563178 MS.gene028311:CDS
TATCACTCTAAAACTTCTGC+TGG 0.536263 4.2:-17563745 None:intergenic
CAATATTCTTGAACAAAATG+AGG 0.537039 4.2:+17561451 MS.gene028311:CDS
GTTCGGAAAACATTCACTGC+AGG 0.544889 4.2:+17562658 MS.gene028311:CDS
ACAAAAGACGTCAACCGACT+GGG 0.550284 4.2:+17561678 MS.gene028311:CDS
TCAAGATGCTGCGATGGCAA+AGG 0.551791 4.2:+17562378 MS.gene028311:CDS
CTCTCATCGTCGCATATCTG+AGG 0.552258 4.2:+17561844 MS.gene028311:CDS
TCACTAAAGTATGGTGAAGT+GGG 0.555591 4.2:+17562016 MS.gene028311:CDS
GCATGAATATATGTCCAAGC+AGG 0.557373 4.2:+17561922 MS.gene028311:CDS
TAGTCATGCTTTGAAAGTGT+TGG 0.559284 4.2:+17563086 MS.gene028311:CDS
TTTCACTTGATCGAAAGATA+GGG 0.567458 4.2:+17561747 MS.gene028311:CDS
AACACTATATACTAAAGCGA+TGG 0.568133 4.2:+17563136 MS.gene028311:CDS
TCAACCGACTGGGGAGTCTA+CGG 0.568520 4.2:+17561688 MS.gene028311:CDS
GCTCGAATGCTCGTCCAAGC+CGG 0.569311 4.2:+17562862 MS.gene028311:CDS
AAGTTCCATGTGGAATTCCT+CGG 0.569361 4.2:-17563699 None:intergenic
GGAAGTAATCAAGATTGGAA+AGG 0.571376 4.2:+17563177 MS.gene028311:CDS
TATATGTCCAAGCAGGTTGG+TGG 0.571492 4.2:+17561929 MS.gene028311:CDS
GACAGTTATGGGAAATCCTA+CGG 0.576210 4.2:+17563011 MS.gene028311:CDS
CACTTTAAAAGAGCTGTCAA+TGG 0.576395 4.2:+17562769 MS.gene028311:intron
ACACAACCACCTTCTTCAGC+CGG 0.580649 4.2:+17561799 MS.gene028311:CDS
TAAAGAGAAAGAAGCCAGCA+TGG 0.582901 4.2:+17563428 MS.gene028311:CDS
AACGGATCAAGATGCTGCGA+TGG 0.589430 4.2:+17562372 MS.gene028311:CDS
CTCACTAAAGTATGGTGAAG+TGG 0.589479 4.2:+17562015 MS.gene028311:CDS
GTTCAGGTAACCGTATCACT+TGG 0.589853 4.2:+17563399 MS.gene028311:CDS
AGATTCACATGCTACAAAGA+AGG 0.592653 4.2:+17561641 MS.gene028311:CDS
CTTGAACAAAATGAGGAACA+TGG 0.594103 4.2:+17561458 MS.gene028311:CDS
AAATCTTGGATTGAGAAAGA+AGG 0.598872 4.2:+17563516 MS.gene028311:CDS
ACCGGGTGTAATGCACGTAT+GGG 0.599839 4.2:+17561722 MS.gene028311:CDS
AAAGCCTAAAACCATTCTAA+CGG 0.600846 4.2:+17562354 MS.gene028311:CDS
GGATATGATATTTGAATCCG+AGG 0.604669 4.2:+17561514 MS.gene028311:CDS
TGAAATCCCATTTCATGAAG+CGG 0.605325 4.2:+17563220 MS.gene028311:CDS
GCCCATACGTGCATTACACC+CGG 0.608109 4.2:-17561723 None:intergenic
CAGCATGGAAACCATTGTCA+AGG 0.609710 4.2:+17563443 MS.gene028311:CDS
GAATATATGTCCAAGCAGGT+TGG 0.614674 4.2:+17561926 MS.gene028311:CDS
ATATGTCCAAGCAGGTTGGT+GGG 0.619945 4.2:+17561930 MS.gene028311:CDS
GCAGGTTGGTGGGATAGAGA+TGG 0.629905 4.2:+17561940 MS.gene028311:CDS
TCAAATATCATATCCAAGCG+AGG 0.630717 4.2:-17561506 None:intergenic
AAAGAGCTGTCAATGGTAAG+CGG 0.639455 4.2:+17562776 MS.gene028311:intron
ACTCTTCCGGCTGAAGAAGG+TGG 0.641594 4.2:-17561805 None:intergenic
TCGAAAGATAGGGAAATACA+AGG 0.644061 4.2:+17561757 MS.gene028311:CDS
CTACGGCAGAAACTAGAACC+GGG 0.648731 4.2:+17561705 MS.gene028311:CDS
AGCGATGGACAAGAGAGGCA+AGG 0.649888 4.2:+17563151 MS.gene028311:CDS
ATTATGATAATGTAAAAGAG+AGG 0.650123 4.2:+17562989 MS.gene028311:CDS
TGTCGTAAATTTGAGACACA+CGG 0.654078 4.2:+17563054 MS.gene028311:CDS
AGTCCAACAAATACTCCCAA+CGG 0.659244 4.2:-17562223 None:intergenic
AATATTTGAGGAATTCCAAG+AGG 0.661269 4.2:+17562903 MS.gene028311:CDS
GGATGGAGAATCAAGTGGAA+TGG 0.662094 4.2:+17563377 MS.gene028311:CDS
AGCAGCCGAGGAATTCCACA+TGG 0.662345 4.2:+17563694 MS.gene028311:intron
ATCCAAGCGAGGTTTGATGT+CGG 0.664750 4.2:-17561495 None:intergenic
TCTGCCGTAGACTCCCCAGT+CGG 0.665919 4.2:-17561692 None:intergenic
CAAAAGACGTCAACCGACTG+GGG 0.676456 4.2:+17561679 MS.gene028311:CDS
TGTGTGGATGGAGAATCAAG+TGG 0.678307 4.2:+17563372 MS.gene028311:CDS
ACTAAAGCGATGGACAAGAG+AGG 0.678959 4.2:+17563146 MS.gene028311:CDS
GGAATTCCAAGAGGAATACG+AGG 0.680926 4.2:+17562912 MS.gene028311:CDS
AAATTCATAAGCATCTACCT+CGG 0.685544 4.2:-17561531 None:intergenic
TTGCTGCAGAAAGTTCCATG+TGG 0.769039 4.2:-17563709 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGAGTATAGTAAAAGAAT+TGG + chr4.2:17561557-17561576 MS.gene028311:CDS 20.0%
!! TATAGTAAAAGAATTGGATT+TGG + chr4.2:17561563-17561582 MS.gene028311:CDS 20.0%
!! TATTTAAATCTGACTTCAAA+TGG - chr4.2:17562732-17562751 None:intergenic 20.0%
!!! AGCAAATAACAAACATATTT+TGG + chr4.2:17562113-17562132 MS.gene028311:CDS 20.0%
!!! ATATATTCATGCAAATCTTT+TGG - chr4.2:17561914-17561933 None:intergenic 20.0%
!!! ATTATGATAATGTAAAAGAG+AGG + chr4.2:17562989-17563008 MS.gene028311:CDS 20.0%
!!! GCAAATAACAAACATATTTT+GGG + chr4.2:17562114-17562133 MS.gene028311:CDS 20.0%
!!! GTATTTGTTTCTCTTTTTTT+GGG - chr4.2:17563568-17563587 None:intergenic 20.0%
!!! TGTATTTGTTTCTCTTTTTT+TGG - chr4.2:17563569-17563588 None:intergenic 20.0%
!!! TTTCTTTTATGACTTTCAAA+TGG + chr4.2:17562081-17562100 MS.gene028311:CDS 20.0%
!!! TTTTCGATTTAAGGAAAAAT+GGG + chr4.2:17562624-17562643 MS.gene028311:intron 20.0%
!!! TTTTTCGATTTAAGGAAAAA+TGG + chr4.2:17562623-17562642 MS.gene028311:intron 20.0%
! ACAAGCATCACAAATTATAA+TGG + chr4.2:17561868-17561887 MS.gene028311:CDS 25.0%
! ATATAAGACAAAACGCTTTA+AGG + chr4.2:17562449-17562468 MS.gene028311:CDS 25.0%
! CAAAATAGTTGAAGATATGT+TGG + chr4.2:17563341-17563360 MS.gene028311:CDS 25.0%
! CAAGCATCACAAATTATAAT+GGG + chr4.2:17561869-17561888 MS.gene028311:CDS 25.0%
! CAATATTCTTGAACAAAATG+AGG + chr4.2:17561451-17561470 MS.gene028311:CDS 25.0%
! CACAAATGATCAATGATTAT+GGG + chr4.2:17562143-17562162 MS.gene028311:CDS 25.0%
! TGAAAGTCATAAAAGAAAGA+TGG - chr4.2:17562079-17562098 None:intergenic 25.0%
! TTGTTGGACTTAATAATCAT+CGG + chr4.2:17562236-17562255 MS.gene028311:CDS 25.0%
! TTTATCATTTCCAAGTGATA+CGG - chr4.2:17563412-17563431 None:intergenic 25.0%
!! AAGATGAATTTTTGAATGCA+TGG + chr4.2:17562548-17562567 MS.gene028311:intron 25.0%
!! AGACAATTCTGTTGATAAAT+TGG + chr4.2:17561424-17561443 MS.gene028311:CDS 25.0%
!! GGAATTCCTCAAATATTTTT+GGG - chr4.2:17562900-17562919 None:intergenic 25.0%
!! TGGAATTCCTCAAATATTTT+TGG - chr4.2:17562901-17562920 None:intergenic 25.0%
!!! GAATTCCTCAAATATTTTTG+GGG - chr4.2:17562899-17562918 None:intergenic 25.0%
!!! GCATATGATTTTTCGATTTA+AGG + chr4.2:17562615-17562634 MS.gene028311:intron 25.0%
AAACGAAGCAAAATAGTGTA+AGG - chr4.2:17563605-17563624 None:intergenic 30.0%
AAAGCCTAAAACCATTCTAA+CGG + chr4.2:17562354-17562373 MS.gene028311:CDS 30.0%
AAATCTTGGATTGAGAAAGA+AGG + chr4.2:17563516-17563535 MS.gene028311:CDS 30.0%
AAATTCATAAGCATCTACCT+CGG - chr4.2:17561534-17561553 None:intergenic 30.0%
AACACTATATACTAAAGCGA+TGG + chr4.2:17563136-17563155 MS.gene028311:CDS 30.0%
AATATTTGAGGAATTCCAAG+AGG + chr4.2:17562903-17562922 MS.gene028311:CDS 30.0%
ACAAGACAAATAAGGGTTAT+CGG + chr4.2:17562200-17562219 MS.gene028311:CDS 30.0%
ACCACATACAAGACAAATAA+GGG + chr4.2:17562193-17562212 MS.gene028311:CDS 30.0%
ACCTAGATTGAAAATGAAGA+AGG + chr4.2:17562840-17562859 MS.gene028311:CDS 30.0%
ATATCAAGACACTTGCATAA+AGG + chr4.2:17562936-17562955 MS.gene028311:CDS 30.0%
ATCAATGATTATGGGTACTT+TGG + chr4.2:17562151-17562170 MS.gene028311:CDS 30.0%
ATGTCCTCCAATGATTAAAT+TGG + chr4.2:17563254-17563273 MS.gene028311:CDS 30.0%
ATTTCACTTGATCGAAAGAT+AGG + chr4.2:17561746-17561765 MS.gene028311:CDS 30.0%
CTAAAAAACGAGCTAAATCT+TGG + chr4.2:17563502-17563521 MS.gene028311:CDS 30.0%
GAAGTAATCAAGATTGGAAA+GGG + chr4.2:17563178-17563197 MS.gene028311:CDS 30.0%
GAATAAAAGCAAAAAAGACG+GGG + chr4.2:17561604-17561623 MS.gene028311:CDS 30.0%
GCACAAATGATCAATGATTA+TGG + chr4.2:17562142-17562161 MS.gene028311:CDS 30.0%
GCTTACTTTGTTGATATAGA+TGG - chr4.2:17562483-17562502 None:intergenic 30.0%
GGAATAAAAGCAAAAAAGAC+GGG + chr4.2:17561603-17561622 MS.gene028311:CDS 30.0%
GGGAATAAAAGCAAAAAAGA+CGG + chr4.2:17561602-17561621 MS.gene028311:CDS 30.0%
TCAAATAACCACTGAAAAGA+AGG - chr4.2:17562307-17562326 None:intergenic 30.0%
TTACACCCCAAAAATATTTG+AGG + chr4.2:17562891-17562910 MS.gene028311:CDS 30.0%
TTTCACTTGATCGAAAGATA+GGG + chr4.2:17561747-17561766 MS.gene028311:CDS 30.0%
! AAACAATACCTTCTTTTCAG+TGG + chr4.2:17562296-17562315 MS.gene028311:CDS 30.0%
! AGAATTTGCTTCGAACAAAA+AGG + chr4.2:17561987-17562006 MS.gene028311:CDS 30.0%
! ATTATAATGGGAGATGAATC+TGG + chr4.2:17561881-17561900 MS.gene028311:CDS 30.0%
! ATTTTGCTTCGTTTCATTAG+TGG + chr4.2:17563611-17563630 MS.gene028311:intron 30.0%
! CATATCTTCAACTATTTTGC+TGG - chr4.2:17563340-17563359 None:intergenic 30.0%
! GAAACTTTTCTCAAAGCAAT+GGG + chr4.2:17562325-17562344 MS.gene028311:CDS 30.0%
! TGAAACTTTTCTCAAAGCAA+TGG + chr4.2:17562324-17562343 MS.gene028311:CDS 30.0%
! TTTTGCTTCGTTTCATTAGT+GGG + chr4.2:17563612-17563631 MS.gene028311:intron 30.0%
!! ACTTGTTGAGCATAATGTTT+TGG + chr4.2:17562579-17562598 MS.gene028311:intron 30.0%
!!! AGATTTAGCTCGTTTTTTAG+TGG - chr4.2:17563502-17563521 None:intergenic 30.0%
!!! ATAATGTTTTGGAAGGTTCA+TGG + chr4.2:17562590-17562609 MS.gene028311:intron 30.0%
AGATTCACATGCTACAAAGA+AGG + chr4.2:17561641-17561660 MS.gene028311:CDS 35.0%
ATCATGTGAACATACTCTTC+CGG - chr4.2:17561821-17561840 None:intergenic 35.0%
ATGCATAGATCTACATGAAG+AGG + chr4.2:17562525-17562544 MS.gene028311:intron 35.0%
CACCACATACAAGACAAATA+AGG + chr4.2:17562192-17562211 MS.gene028311:CDS 35.0%
CGCTTTAGTATATAGTGTTC+AGG - chr4.2:17563135-17563154 None:intergenic 35.0%
CTGTTGGCCAATTTAATCAT+TGG - chr4.2:17563264-17563283 None:intergenic 35.0%
CTTGAACAAAATGAGGAACA+TGG + chr4.2:17561458-17561477 MS.gene028311:CDS 35.0%
GATTTAAGGAAAAATGGGCA+TGG + chr4.2:17562629-17562648 MS.gene028311:intron 35.0%
GGAAGTAATCAAGATTGGAA+AGG + chr4.2:17563177-17563196 MS.gene028311:CDS 35.0%
GGATATGATATTTGAATCCG+AGG + chr4.2:17561514-17561533 MS.gene028311:CDS 35.0%
GTAAAAGAGAGGACAGTTAT+GGG + chr4.2:17563000-17563019 MS.gene028311:CDS 35.0%
TATGTTGGCCAAAAAATGTG+TGG + chr4.2:17563356-17563375 MS.gene028311:CDS 35.0%
TCAAATATCATATCCAAGCG+AGG - chr4.2:17561509-17561528 None:intergenic 35.0%
TCACTAAAGTATGGTGAAGT+GGG + chr4.2:17562016-17562035 MS.gene028311:CDS 35.0%
TCGAAAGATAGGGAAATACA+AGG + chr4.2:17561757-17561776 MS.gene028311:CDS 35.0%
TGAAATCCCATTTCATGAAG+CGG + chr4.2:17563220-17563239 MS.gene028311:CDS 35.0%
TGTAAAAGAGAGGACAGTTA+TGG + chr4.2:17562999-17563018 MS.gene028311:CDS 35.0%
TTGGCCAATTTAATCATTGG+AGG - chr4.2:17563261-17563280 None:intergenic 35.0%
TTGGTATTCGTCGAGAATAT+GGG + chr4.2:17561582-17561601 MS.gene028311:CDS 35.0%
TTTGCTTCGAACAAAAAGGA+TGG + chr4.2:17561991-17562010 MS.gene028311:CDS 35.0%
TTTGCTTCGTTTCATTAGTG+GGG + chr4.2:17563613-17563632 MS.gene028311:intron 35.0%
TTTGGTATTCGTCGAGAATA+TGG + chr4.2:17561581-17561600 MS.gene028311:CDS 35.0%
! ACCCTTATTTGTCTTGTATG+TGG - chr4.2:17562197-17562216 None:intergenic 35.0%
! CACTTTAAAAGAGCTGTCAA+TGG + chr4.2:17562769-17562788 MS.gene028311:intron 35.0%
! CATCGGCAAACAATCATTTT+TGG + chr4.2:17562253-17562272 MS.gene028311:CDS 35.0%
! CATTTCATGGATTGTGTACT+TGG + chr4.2:17562425-17562444 MS.gene028311:CDS 35.0%
! GCCTTCTTCATTTTCAATCT+AGG - chr4.2:17562844-17562863 None:intergenic 35.0%
! TGATCCGTTAGAATGGTTTT+AGG - chr4.2:17562361-17562380 None:intergenic 35.0%
! TGTCGTAAATTTGAGACACA+CGG + chr4.2:17563054-17563073 MS.gene028311:CDS 35.0%
!! GTTGAGCATAATGTTTTGGA+AGG + chr4.2:17562583-17562602 MS.gene028311:intron 35.0%
!! TAGTCATGCTTTGAAAGTGT+TGG + chr4.2:17563086-17563105 MS.gene028311:CDS 35.0%
!!! CAAGAAACTTTTAGTTCCGT+AGG - chr4.2:17563030-17563049 None:intergenic 35.0%
!!! TTCTCCATCCACACATTTTT+TGG - chr4.2:17563367-17563386 None:intergenic 35.0%
!!! TTTTGGGGTGTAAACATTTC+CGG - chr4.2:17562884-17562903 None:intergenic 35.0%
AAAGAGCTGTCAATGGTAAG+CGG + chr4.2:17562776-17562795 MS.gene028311:intron 40.0%
AAGTTCCATGTGGAATTCCT+CGG - chr4.2:17563702-17563721 None:intergenic 40.0%
AATGGGCATGGACTTATGTT+CGG + chr4.2:17562641-17562660 MS.gene028311:CDS 40.0%
ACTGTACCGCTTCATGAAAT+GGG - chr4.2:17563229-17563248 None:intergenic 40.0%
AGAATCAAGTGGAATGGTTC+AGG + chr4.2:17563383-17563402 MS.gene028311:CDS 40.0%
AGCATCTTGATCCGTTAGAA+TGG - chr4.2:17562368-17562387 None:intergenic 40.0%
AGTCCAACAAATACTCCCAA+CGG - chr4.2:17562226-17562245 None:intergenic 40.0%
CACAATCCATGAAATGTCTC+TGG - chr4.2:17562421-17562440 None:intergenic 40.0%
CTCACTAAAGTATGGTGAAG+TGG + chr4.2:17562015-17562034 MS.gene028311:CDS 40.0%
GAAACCATTGTCAAGGCAAA+AGG + chr4.2:17563450-17563469 MS.gene028311:CDS 40.0%
GAATATATGTCCAAGCAGGT+TGG + chr4.2:17561926-17561945 MS.gene028311:CDS 40.0%
GACAGTTATGGGAAATCCTA+CGG + chr4.2:17563011-17563030 MS.gene028311:CDS 40.0%
GATATTCCTCGTATTCCTCT+TGG - chr4.2:17562921-17562940 None:intergenic 40.0%
GCATGAATATATGTCCAAGC+AGG + chr4.2:17561922-17561941 MS.gene028311:CDS 40.0%
GCTGGACTCTTCATAAACTT+TGG - chr4.2:17563322-17563341 None:intergenic 40.0%
GGTTGGGAAGTAATCAAGAT+TGG + chr4.2:17563172-17563191 MS.gene028311:CDS 40.0%
GTTATGCCAGAGACATTTCA+TGG + chr4.2:17562412-17562431 MS.gene028311:CDS 40.0%
TAAAGAGAAAGAAGCCAGCA+TGG + chr4.2:17563428-17563447 MS.gene028311:CDS 40.0%
TAAATTGGCCAACAGAGCTT+CGG + chr4.2:17563269-17563288 MS.gene028311:CDS 40.0%
TTGGCCAAAAAATGTGTGGA+TGG + chr4.2:17563360-17563379 MS.gene028311:CDS 40.0%
! ACTTTTCTCAAAGCAATGGG+TGG + chr4.2:17562328-17562347 MS.gene028311:CDS 40.0%
! AGACACTTGCATAAAGGTGT+TGG + chr4.2:17562942-17562961 MS.gene028311:CDS 40.0%
! AGGATGGACTCACTAAAGTA+TGG + chr4.2:17562007-17562026 MS.gene028311:CDS 40.0%
! TATGCCTTTTGCCTTGACAA+TGG - chr4.2:17563457-17563476 None:intergenic 40.0%
! TTTTTATATGTCAGCAGCCG+AGG + chr4.2:17563682-17563701 MS.gene028311:intron 40.0%
!! TGCATAAAGGTGTTGGAAGA+AGG + chr4.2:17562949-17562968 MS.gene028311:CDS 40.0%
AAATAAGGGTTATCGGCCGT+TGG + chr4.2:17562207-17562226 MS.gene028311:CDS 45.0%
AATAAGGGTTATCGGCCGTT+GGG + chr4.2:17562208-17562227 MS.gene028311:CDS 45.0%
ACAAAAGACGTCAACCGACT+GGG + chr4.2:17561678-17561697 MS.gene028311:CDS 45.0%
ACTAAAGCGATGGACAAGAG+AGG + chr4.2:17563146-17563165 MS.gene028311:CDS 45.0%
ATCCAAGCGAGGTTTGATGT+CGG - chr4.2:17561498-17561517 None:intergenic 45.0%
ATGAGATTCCGAAGCTCTGT+TGG - chr4.2:17563280-17563299 None:intergenic 45.0%
CACTGTACCGCTTCATGAAA+TGG - chr4.2:17563230-17563249 None:intergenic 45.0%
CAGCATGGAAACCATTGTCA+AGG + chr4.2:17563443-17563462 MS.gene028311:CDS 45.0%
CTTGACAATGGTTTCCATGC+TGG - chr4.2:17563445-17563464 None:intergenic 45.0%
GGAATTCCAAGAGGAATACG+AGG + chr4.2:17562912-17562931 MS.gene028311:CDS 45.0%
GGATGGAGAATCAAGTGGAA+TGG + chr4.2:17563377-17563396 MS.gene028311:CDS 45.0%
GTTCAGGTAACCGTATCACT+TGG + chr4.2:17563399-17563418 MS.gene028311:CDS 45.0%
GTTCGGAAAACATTCACTGC+AGG + chr4.2:17562658-17562677 MS.gene028311:CDS 45.0%
TCTACGGCAGAAACTAGAAC+CGG + chr4.2:17561704-17561723 MS.gene028311:CDS 45.0%
TGTGTGGATGGAGAATCAAG+TGG + chr4.2:17563372-17563391 MS.gene028311:CDS 45.0%
TTGCTGCAGAAAGTTCCATG+TGG - chr4.2:17563712-17563731 None:intergenic 45.0%
! TATATGTCCAAGCAGGTTGG+TGG + chr4.2:17561929-17561948 MS.gene028311:CDS 45.0%
!! ATATGTCCAAGCAGGTTGGT+GGG + chr4.2:17561930-17561949 MS.gene028311:CDS 45.0%
!! ACATATAAAAATTAAAAATT+CGG - chr4.2:17563673-17563692 None:intergenic 5.0%
AACCGGGTGTAATGCACGTA+TGG + chr4.2:17561721-17561740 MS.gene028311:CDS 50.0%
AACGGATCAAGATGCTGCGA+TGG + chr4.2:17562372-17562391 MS.gene028311:CDS 50.0%
ACACAACCACCTTCTTCAGC+CGG + chr4.2:17561799-17561818 MS.gene028311:CDS 50.0%
ACCGGGTGTAATGCACGTAT+GGG + chr4.2:17561722-17561741 MS.gene028311:CDS 50.0%
ATGGACAAGAGAGGCAAGGT+TGG + chr4.2:17563155-17563174 MS.gene028311:CDS 50.0%
CAAAAGACGTCAACCGACTG+GGG + chr4.2:17561679-17561698 MS.gene028311:CDS 50.0%
CAAGATGCTGCGATGGCAAA+GGG + chr4.2:17562379-17562398 MS.gene028311:CDS 50.0%
CATACTCTTCCGGCTGAAGA+AGG - chr4.2:17561811-17561830 None:intergenic 50.0%
CTACGGCAGAAACTAGAACC+GGG + chr4.2:17561705-17561724 MS.gene028311:CDS 50.0%
CTCTCATCGTCGCATATCTG+AGG + chr4.2:17561844-17561863 MS.gene028311:CDS 50.0%
GACAAAAGACGTCAACCGAC+TGG + chr4.2:17561677-17561696 MS.gene028311:CDS 50.0%
GGGTGTAAACATTTCCGGCT+TGG - chr4.2:17562879-17562898 None:intergenic 50.0%
TCAAGATGCTGCGATGGCAA+AGG + chr4.2:17562378-17562397 MS.gene028311:CDS 50.0%
TGGACAAGAGAGGCAAGGTT+GGG + chr4.2:17563156-17563175 MS.gene028311:CDS 50.0%
! GTTGGTGGGATAGAGATGGT+TGG + chr4.2:17561944-17561963 MS.gene028311:CDS 50.0%
ACTCTTCCGGCTGAAGAAGG+TGG - chr4.2:17561808-17561827 None:intergenic 55.0%
AGCAGCCGAGGAATTCCACA+TGG + chr4.2:17563694-17563713 MS.gene028311:intron 55.0%
AGCGATGGACAAGAGAGGCA+AGG + chr4.2:17563151-17563170 MS.gene028311:CDS 55.0%
CTCTATCCCACCAACCTGCT+TGG - chr4.2:17561939-17561958 None:intergenic 55.0%
GCCCATACGTGCATTACACC+CGG - chr4.2:17561726-17561745 None:intergenic 55.0%
TCAACCGACTGGGGAGTCTA+CGG + chr4.2:17561688-17561707 MS.gene028311:CDS 55.0%
TGCCGACATCAAACCTCGCT+TGG + chr4.2:17561493-17561512 MS.gene028311:CDS 55.0%
!! CGGCCGTTGGGAGTATTTGT+TGG + chr4.2:17562220-17562239 MS.gene028311:CDS 55.0%
!! GCAGGTTGGTGGGATAGAGA+TGG + chr4.2:17561940-17561959 MS.gene028311:CDS 55.0%
GCTCGAATGCTCGTCCAAGC+CGG + chr4.2:17562862-17562881 MS.gene028311:CDS 60.0%
TCTGCCGTAGACTCCCCAGT+CGG - chr4.2:17561695-17561714 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 17561401 17563760 17561401 ID=MS.gene028311
chr4.2 mRNA 17561401 17563760 17561401 ID=MS.gene028311.t1;Parent=MS.gene028311
chr4.2 exon 17561401 17562496 17561401 ID=MS.gene028311.t1.exon1;Parent=MS.gene028311.t1
chr4.2 CDS 17561401 17562496 17561401 ID=cds.MS.gene028311.t1;Parent=MS.gene028311.t1
chr4.2 exon 17562637 17562679 17562637 ID=MS.gene028311.t1.exon2;Parent=MS.gene028311.t1
chr4.2 CDS 17562637 17562679 17562637 ID=cds.MS.gene028311.t1;Parent=MS.gene028311.t1
chr4.2 exon 17562780 17563537 17562780 ID=MS.gene028311.t1.exon3;Parent=MS.gene028311.t1
chr4.2 CDS 17562780 17563537 17562780 ID=cds.MS.gene028311.t1;Parent=MS.gene028311.t1
chr4.2 exon 17563699 17563760 17563699 ID=MS.gene028311.t1.exon4;Parent=MS.gene028311.t1
chr4.2 CDS 17563699 17563760 17563699 ID=cds.MS.gene028311.t1;Parent=MS.gene028311.t1
Gene Sequence

>MS.gene028311

ATGAATTCTTCGTCTATTTTGGTAGACAATTCTGTTGATAAATTGGTAAACAATATTCTTGAACAAAATGAGGAACATGGAAAAGAAAGTATTGCCGACATCAAACCTCGCTTGGATATGATATTTGAATCCGAGGTAGATGCTTATGAATTTTATAATGAGTATAGTAAAAGAATTGGATTTGGTATTCGTCGAGAATATGGGAATAAAAGCAAAAAAGACGGGGTTTTAACTTCAAGAAGATTCACATGCTACAAAGAAGGTACACGAAGTGTTGACAAAAGACGTCAACCGACTGGGGAGTCTACGGCAGAAACTAGAACCGGGTGTAATGCACGTATGGGCATTTCACTTGATCGAAAGATAGGGAAATACAAGGTTGTTGACTTTATACTAGAACACAACCACCTTCTTCAGCCGGAAGAGTATGTTCACATGATTCGCTCTCATCGTCGCATATCTGAGGCACAAGCATCACAAATTATAATGGGAGATGAATCTGGATTAAGACCAAAAGATTTGCATGAATATATGTCCAAGCAGGTTGGTGGGATAGAGATGGTTGGTTTTACAAGAACAGACGTTAAGAATTTGCTTCGAACAAAAAGGATGGACTCACTAAAGTATGGTGAAGTGGGTGCATTGATGACTTATTTCAAACATGAAAGCGAAAATCCATCTTTCTTTTATGACTTTCAAATGGATGTTGAAGAGCAAATAACAAACATATTTTGGGCAGATGCACAAATGATCAATGATTATGGGTACTTTGGTGATGTCATCACTTTTGACACCACATACAAGACAAATAAGGGTTATCGGCCGTTGGGAGTATTTGTTGGACTTAATAATCATCGGCAAACAATCATTTTTGGTGCAACATTGTTATATGATGAAACAATACCTTCTTTTCAGTGGTTATTTGAAACTTTTCTCAAAGCAATGGGTGGAAAAAAGCCTAAAACCATTCTAACGGATCAAGATGCTGCGATGGCAAAGGGTATTTCTTTAGTTATGCCAGAGACATTTCATGGATTGTGTACTTGGCATATAAGACAAAACGCTTTAAGGCATGTGAACCATCTATATCAACAAAGTAAGCACTTTTCTTCAGATTTTGAAGCATGCATAGATCTACATGAAGAGGAAGATGAATTTTTGAATGCATGGAACTCTTTACTTGTTGAGCATAATGTTTTGGAAGGTTCATGGTTGCATATGATTTTTCGATTTAAGGAAAAATGGGCATGGACTTATGTTCGGAAAACATTCACTGCAGGTATGAGATCTACGCAATTAAGTGAAAGCTTTAATGCTGATTTGAAAAACCATTTGAAGTCAGATTTAAATATAGTACAATTTTTTACACACTTTAAAAGAGCTGTCAATGGTAAGCGGAACAATGAGTCAGAAGCTGAATATGAGTCAAGACATAAGCTACCTAGATTGAAAATGAAGAAGGCTCGAATGCTCGTCCAAGCCGGAAATGTTTACACCCCAAAAATATTTGAGGAATTCCAAGAGGAATACGAGGAATATCAAGACACTTGCATAAAGGTGTTGGAAGAAGGTTTGTATGCTGTCACAAATTATGATAATGTAAAAGAGAGGACAGTTATGGGAAATCCTACGGAACTAAAAGTTTCTTGTGATTGTCGTAAATTTGAGACACACGGCATCTTATGTAGTCATGCTTTGAAAGTGTTGGATGTCATGAATGTTAAGTTAATTCCTGAACACTATATACTAAAGCGATGGACAAGAGAGGCAAGGTTGGGAAGTAATCAAGATTGGAAAGGGATGCATGTTGAACTAGACATGAAATCCCATTTCATGAAGCGGTACAGTGAGTTATGTCCTCCAATGATTAAATTGGCCAACAGAGCTTCGGAATCTCATGAAACTTACACTTTTTTGTCCAAAGTTTATGAAGAGTCCAGCAAAATAGTTGAAGATATGTTGGCCAAAAAATGTGTGGATGGAGAATCAAGTGGAATGGTTCAGGTAACCGTATCACTTGGAAATGATAAAGAGAAAGAAGCCAGCATGGAAACCATTGTCAAGGCAAAAGGCATAAAGAAGCGAGATTGTTCACGTTCCACTAAAAAACGAGCTAAATCTTGGATTGAGAAAGAAGGTAAAAAGAGAAACAAATACATTCAGTCCCAAAAAAAGAGAAACAAATACATTTCAGTGTCATACCTTACACTATTTTGCTTCGTTTCATTAGTGGGGCAGAATCAGCAACATAATTTTTATATGTTAGCAGCCGAATTTTTAATTTTTATATGTCAGCAGCCGAGGAATTCCACATGGAACTTTCTGCAGCAACAGAATTTTTATGCCAGCAGAAGTTTTAG
Protein sequence

>MS.gene028311.t1

MNSSSILVDNSVDKLVNNILEQNEEHGKESIADIKPRLDMIFESEVDAYEFYNEYSKRIGFGIRREYGNKSKKDGVLTSRRFTCYKEGTRSVDKRRQPTGESTAETRTGCNARMGISLDRKIGKYKVVDFILEHNHLLQPEEYVHMIRSHRRISEAQASQIIMGDESGLRPKDLHEYMSKQVGGIEMVGFTRTDVKNLLRTKRMDSLKYGEVGALMTYFKHESENPSFFYDFQMDVEEQITNIFWADAQMINDYGYFGDVITFDTTYKTNKGYRPLGVFVGLNNHRQTIIFGATLLYDETIPSFQWLFETFLKAMGGKKPKTILTDQDAAMAKGISLVMPETFHGLCTWHIRQNALRHVNHLYQQRKMGMDLCSENIHCRAVNGKRNNESEAEYESRHKLPRLKMKKARMLVQAGNVYTPKIFEEFQEEYEEYQDTCIKVLEEGLYAVTNYDNVKERTVMGNPTELKVSCDCRKFETHGILCSHALKVLDVMNVKLIPEHYILKRWTREARLGSNQDWKGMHVELDMKSHFMKRYSELCPPMIKLANRASESHETYTFLSKVYEESSKIVEDMLAKKCVDGESSGMVQVTVSLGNDKEKEASMETIVKAKGIKKRDCSRSTKKRAKSWIEKEAEEFHMELSAATEFLCQQKF