AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028314

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028314.t1 MTR_4g094632 91.772 158 13 0 1 158 1 158 2.48e-103 293
MS.gene028314.t1 MTR_4g094638 66.854 178 53 2 1 172 1 178 9.12e-72 214
MS.gene028314.t1 MTR_8g079502 42.073 164 83 3 1 152 1 164 2.25e-33 117
MS.gene028314.t1 MTR_1g047550 39.665 179 95 4 1 166 1 179 2.85e-32 114
MS.gene028314.t1 MTR_4g131030 38.122 181 83 3 1 152 1 181 4.36e-30 109
MS.gene028314.t1 MTR_8g046350 38.037 163 85 5 1 152 1 158 1.83e-29 106
MS.gene028314.t1 MTR_1g063160 38.415 164 89 2 1 152 1 164 5.74e-29 105
MS.gene028314.t1 MTR_8g051580 40.244 164 86 4 1 152 1 164 9.62e-29 105
MS.gene028314.t1 MTR_3g080940 39.024 164 83 4 1 147 8 171 2.65e-28 104
MS.gene028314.t1 MTR_1g054265 39.024 164 88 3 1 152 1 164 5.90e-27 100
MS.gene028314.t1 MTR_5g045560 42.982 114 62 2 1 112 8 120 1.85e-23 92.8
MS.gene028314.t1 MTR_3g052920 42.478 113 62 2 2 112 9 120 2.53e-23 92.4
MS.gene028314.t1 MTR_1g106070 41.593 113 63 2 2 112 9 120 3.23e-23 90.9
MS.gene028314.t1 MTR_3g052870 33.918 171 101 4 2 167 9 172 1.06e-22 90.5
MS.gene028314.t1 MTR_1g108510 41.509 106 60 1 2 105 9 114 2.09e-22 90.1
MS.gene028314.t1 MTR_8g043650 43.396 106 58 1 2 105 9 114 2.74e-22 89.7
MS.gene028314.t1 MTR_3g030770 39.370 127 68 2 2 126 9 128 3.58e-22 89.4
MS.gene028314.t1 MTR_1g108580 40.566 106 61 1 2 105 9 114 6.55e-22 88.6
MS.gene028314.t1 MTR_1g105920 41.593 113 63 2 2 112 9 120 6.57e-22 89.0
MS.gene028314.t1 MTR_1g105910 42.453 106 59 1 2 105 9 114 1.56e-21 87.8
MS.gene028314.t1 MTR_5g066180 45.370 108 53 3 1 104 1 106 5.43e-21 86.3
MS.gene028314.t1 MTR_2g093190 32.418 182 92 5 2 152 9 190 6.45e-21 85.9
MS.gene028314.t1 MTR_1g012570 35.714 140 81 2 2 139 9 141 1.64e-20 85.1
MS.gene028314.t1 MTR_2g105290 50.000 72 36 0 1 72 11 82 2.64e-20 83.2
MS.gene028314.t1 MTR_5g053390 37.795 127 76 2 2 125 9 135 2.95e-20 84.3
MS.gene028314.t1 MTR_2g030740 38.938 113 66 2 2 112 9 120 1.35e-19 82.8
MS.gene028314.t1 MTR_0121s0080 38.281 128 70 2 2 127 9 129 4.36e-19 81.3
MS.gene028314.t1 MTR_8g086290 46.479 71 38 0 2 72 12 82 5.08e-19 79.7
MS.gene028314.t1 MTR_0121s0100 38.614 101 60 1 2 100 9 109 1.63e-18 77.4
MS.gene028314.t1 MTR_7g028448 32.000 150 86 4 17 158 27 168 1.98e-18 78.2
MS.gene028314.t1 MTR_4g036915 32.000 150 86 4 17 158 27 168 1.98e-18 78.2
MS.gene028314.t1 MTR_5g032520 36.029 136 79 4 1 132 1 132 3.97e-18 78.6
MS.gene028314.t1 MTR_1g077360 38.835 103 61 1 2 102 9 111 7.03e-18 78.2
MS.gene028314.t1 MTR_1g075600 38.095 105 63 1 2 104 9 113 7.10e-18 78.2
MS.gene028314.t1 MTR_2g009890 50.725 69 34 0 1 69 1 69 9.51e-18 78.6
MS.gene028314.t1 MTR_3g030780 36.449 107 66 1 2 106 9 115 1.00e-17 75.5
MS.gene028314.t1 MTR_5g032150 35.294 136 80 4 1 132 1 132 2.50e-17 76.6
MS.gene028314.t1 MTR_2g085250 37.500 112 67 2 2 111 9 119 2.60e-17 76.6
MS.gene028314.t1 MTR_2g085280 37.500 112 67 2 2 111 9 119 2.80e-17 76.6
MS.gene028314.t1 MTR_5g055100 37.500 112 67 2 2 111 9 119 3.01e-17 76.6
MS.gene028314.t1 MTR_5g031000 49.275 69 35 0 1 69 1 69 3.37e-17 76.3
MS.gene028314.t1 MTR_5g031000 49.275 69 35 0 1 69 1 69 3.65e-17 75.9
MS.gene028314.t1 MTR_7g075870 35.135 111 69 1 1 111 1 108 5.96e-17 75.1
MS.gene028314.t1 MTR_4g093970 31.461 178 103 6 1 171 27 192 1.01e-16 75.1
MS.gene028314.t1 MTR_4g127140 52.778 72 34 0 1 72 11 82 1.12e-16 73.9
MS.gene028314.t1 MTR_3g102570 33.813 139 79 4 1 134 1 131 2.00e-16 75.5
MS.gene028314.t1 MTR_4g109830 36.364 121 73 3 1 120 1 118 2.96e-16 73.6
MS.gene028314.t1 MTR_0003s0590 49.275 69 35 0 1 69 1 69 3.59e-16 73.9
MS.gene028314.t1 MTR_5g066960 53.333 60 28 0 1 60 1 60 4.04e-16 69.3
MS.gene028314.t1 MTR_3g113030 35.484 124 77 2 1 124 1 121 1.13e-15 72.0
MS.gene028314.t1 MTR_1g053070 47.826 69 36 0 1 69 1 69 1.35e-15 72.4
MS.gene028314.t1 MTR_1g105905 38.542 96 57 1 11 104 1 96 1.62e-15 71.6
MS.gene028314.t1 MTR_7g075870 34.821 112 69 2 1 111 1 109 1.83e-15 71.2
MS.gene028314.t1 MTR_8g033270 52.542 59 28 0 1 59 1 59 4.98e-15 70.9
MS.gene028314.t1 MTR_5g021270 50.000 60 30 0 1 60 1 60 8.11e-15 69.7
MS.gene028314.t1 MTR_5g021270 50.000 60 30 0 1 60 1 60 8.14e-15 69.7
MS.gene028314.t1 MTR_5g046870 48.529 68 35 0 1 68 1 68 9.17e-15 67.0
MS.gene028314.t1 MTR_3g005530 31.667 120 63 1 1 101 1 120 1.02e-14 69.3
MS.gene028314.t1 MTR_7g016630 32.258 124 72 3 1 120 1 116 1.13e-14 69.7
MS.gene028314.t1 MTR_7g075850 34.677 124 69 2 1 119 1 117 1.16e-14 69.7
MS.gene028314.t1 MTR_3g452380 44.928 69 38 0 1 69 16 84 1.30e-14 69.7
MS.gene028314.t1 MTR_4g093030 34.722 144 70 6 1 142 1 122 1.68e-14 67.8
MS.gene028314.t1 MTR_1g101970 44.928 69 38 0 1 69 1 69 1.73e-14 68.2
MS.gene028314.t1 MTR_1g038300 50.820 61 30 0 1 61 1 61 3.03e-14 68.2
MS.gene028314.t1 MTR_1g029670 36.207 116 64 4 1 111 1 111 3.96e-14 67.0
MS.gene028314.t1 MTR_7g016600 47.541 61 32 0 1 61 1 61 4.68e-14 68.2
MS.gene028314.t1 MTR_4g036050 32.174 115 75 3 1 114 1 113 5.68e-14 66.6
MS.gene028314.t1 MTR_4g036050 32.174 115 75 3 1 114 1 113 6.44e-14 66.6
MS.gene028314.t1 MTR_4g036050 32.174 115 75 3 1 114 1 113 6.65e-14 66.6
MS.gene028314.t1 MTR_4g036050 32.174 115 75 3 1 114 1 113 7.79e-14 66.6
MS.gene028314.t1 MTR_6g464720 49.180 61 31 0 1 61 1 61 8.54e-14 67.4
MS.gene028314.t1 MTR_6g081890 54.000 100 31 3 44 140 62 149 9.90e-14 65.9
MS.gene028314.t1 MTR_3g088615 45.902 61 33 0 1 61 1 61 1.08e-13 65.9
MS.gene028314.t1 MTR_5g046790 44.262 61 34 0 1 61 1 61 1.11e-13 66.6
MS.gene028314.t1 MTR_4g102530 42.029 69 40 0 1 69 1 69 1.30e-13 66.2
MS.gene028314.t1 MTR_8g033220 42.029 69 40 0 1 69 1 69 1.31e-13 66.6
MS.gene028314.t1 MTR_8g033220 42.029 69 40 0 1 69 1 69 1.46e-13 66.6
MS.gene028314.t1 MTR_2g461710 45.763 59 32 0 1 59 1 59 1.49e-13 62.4
MS.gene028314.t1 MTR_8g066260 41.429 70 40 1 1 69 1 70 1.49e-13 66.6
MS.gene028314.t1 MTR_6g015975 44.286 70 38 1 1 69 1 70 1.67e-13 66.6
MS.gene028314.t1 MTR_4g109810 43.662 71 40 0 1 71 1 71 1.76e-13 66.2
MS.gene028314.t1 MTR_8g087860 35.000 100 62 1 1 100 17 113 2.76e-13 65.9
MS.gene028314.t1 MTR_8g097090 47.541 61 32 0 1 61 1 61 2.92e-13 65.9
MS.gene028314.t1 MTR_3g084980 47.541 61 32 0 1 61 1 61 3.24e-13 65.9
MS.gene028314.t1 MTR_7g062350 35.354 99 62 1 10 106 29 127 3.83e-13 65.5
MS.gene028314.t1 MTR_2g017865 40.580 69 41 0 1 69 16 84 1.24e-12 64.3
MS.gene028314.t1 MTR_3g067910 38.202 89 53 1 19 105 7 95 1.34e-12 61.2
MS.gene028314.t1 MTR_3g067875 50.909 55 27 0 11 65 1 55 2.48e-12 59.7
MS.gene028314.t1 MTR_5g041650 48.148 54 28 0 1 54 38 91 5.37e-12 63.2
MS.gene028314.t1 MTR_4g084740 43.662 71 39 1 1 70 1 71 7.42e-12 62.8
MS.gene028314.t1 MTR_3g109930 30.519 154 85 5 17 154 17 164 9.43e-12 60.5
MS.gene028314.t1 MTR_1g041615 60.465 43 17 0 17 59 21 63 3.25e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028314.t1 AT2G34440 37.791 172 85 4 1 150 1 172 3.95e-29 105
MS.gene028314.t1 AT3G66656 42.857 112 56 2 1 105 1 111 8.68e-24 92.4
MS.gene028314.t1 AT2G24840 40.496 121 57 3 1 113 62 175 1.13e-22 91.3
MS.gene028314.t1 AT1G28460 40.870 115 64 2 2 112 8 122 3.93e-21 85.5
MS.gene028314.t1 AT1G29962 38.462 130 69 3 2 120 8 137 8.05e-21 84.7
MS.gene028314.t1 AT3G57230 41.509 106 57 2 1 102 1 105 9.20e-20 80.9
MS.gene028314.t1 AT2G22540 35.542 166 93 6 1 156 1 162 1.63e-19 82.8
MS.gene028314.t1 AT2G22540 35.542 166 93 6 1 156 1 162 1.63e-19 82.8
MS.gene028314.t1 AT4G24540 41.667 108 59 2 1 104 1 108 2.06e-19 82.0
MS.gene028314.t1 AT3G57230 41.509 106 57 2 1 102 1 105 5.96e-19 81.3
MS.gene028314.t1 AT3G57230 41.509 106 57 2 1 102 1 105 5.96e-19 81.3
MS.gene028314.t1 AT1G28450 39.831 118 64 3 2 112 8 125 1.41e-18 79.0
MS.gene028314.t1 AT4G37940 49.275 69 35 0 1 69 1 69 2.46e-18 79.3
MS.gene028314.t1 AT4G37940 49.275 69 35 0 1 69 1 69 3.24e-18 78.6
MS.gene028314.t1 AT5G60440 38.562 153 77 5 2 139 7 157 5.50e-18 79.3
MS.gene028314.t1 AT4G37940 49.275 69 35 0 1 69 1 69 9.00e-18 76.6
MS.gene028314.t1 AT1G71692 38.655 119 68 3 1 114 1 119 9.92e-18 77.4
MS.gene028314.t1 AT2G45660 33.333 111 71 1 1 111 1 108 1.63e-17 75.5
MS.gene028314.t1 AT1G65360 33.333 111 74 0 1 111 6 116 4.64e-17 75.9
MS.gene028314.t1 AT1G01530 33.117 154 79 3 1 134 6 155 4.79e-17 76.3
MS.gene028314.t1 AT1G47760 33.129 163 83 5 1 149 1 151 5.22e-17 74.7
MS.gene028314.t1 AT4G22950 33.775 151 89 3 1 146 1 145 7.15e-17 74.3
MS.gene028314.t1 AT2G22540 34.545 165 91 6 1 156 1 157 7.94e-17 75.5
MS.gene028314.t1 AT2G45660 33.333 111 71 1 1 111 1 108 8.93e-17 74.7
MS.gene028314.t1 AT3G57390 49.275 69 35 0 1 69 1 69 9.27e-17 75.1
MS.gene028314.t1 AT5G13790 38.235 102 63 0 1 102 1 102 1.68e-16 75.1
MS.gene028314.t1 AT3G57390 49.275 69 35 0 1 69 1 69 1.76e-16 74.7
MS.gene028314.t1 AT4G22950 33.775 151 89 3 1 146 1 145 3.42e-16 73.6
MS.gene028314.t1 AT4G22950 33.775 151 89 3 1 146 1 145 3.42e-16 73.6
MS.gene028314.t1 AT5G13790 38.235 102 60 1 1 102 1 99 3.82e-16 73.9
MS.gene028314.t1 AT4G09960 32.031 128 67 2 1 108 1 128 4.02e-16 73.6
MS.gene028314.t1 AT4G09960 32.031 128 67 2 1 108 2 129 4.13e-16 73.6
MS.gene028314.t1 AT4G09960 32.031 128 67 2 1 108 1 128 4.27e-16 73.2
MS.gene028314.t1 AT5G51870 46.377 69 37 0 1 69 1 69 4.68e-16 72.0
MS.gene028314.t1 AT4G09960 32.031 128 67 2 1 108 11 138 5.12e-16 73.2
MS.gene028314.t1 AT5G10140 52.542 59 28 0 1 59 1 59 5.67e-16 71.6
MS.gene028314.t1 AT5G10140 52.542 59 28 0 1 59 1 59 6.63e-16 72.0
MS.gene028314.t1 AT5G10140 52.542 59 28 0 1 59 1 59 7.19e-16 71.6
MS.gene028314.t1 AT5G51870 46.377 69 37 0 1 69 1 69 7.53e-16 72.4
MS.gene028314.t1 AT4G09960 32.031 128 67 2 1 108 27 154 7.63e-16 73.2
MS.gene028314.t1 AT5G51870 46.377 69 37 0 1 69 24 92 7.67e-16 72.0
MS.gene028314.t1 AT2G14210 55.932 59 26 0 1 59 1 59 8.45e-16 72.8
MS.gene028314.t1 AT5G10140 52.542 59 28 0 1 59 1 59 8.59e-16 72.0
MS.gene028314.t1 AT2G45650 47.826 69 36 0 1 69 1 69 9.46e-16 72.8
MS.gene028314.t1 AT4G09960 32.031 128 67 2 1 108 96 223 9.72e-16 73.6
MS.gene028314.t1 AT2G14210 55.932 59 26 0 1 59 1 59 9.81e-16 72.4
MS.gene028314.t1 AT5G23260 49.315 73 35 1 1 71 1 73 1.13e-15 72.4
MS.gene028314.t1 AT4G36590 37.736 106 59 2 2 100 7 112 1.26e-15 72.4
MS.gene028314.t1 AT5G23260 49.315 73 35 1 1 71 17 89 1.30e-15 72.8
MS.gene028314.t1 AT5G23260 49.315 73 35 1 1 71 1 73 1.32e-15 72.0
MS.gene028314.t1 AT5G23260 49.315 73 35 1 1 71 17 89 1.47e-15 72.4
MS.gene028314.t1 AT5G51870 46.377 69 37 0 1 69 1 69 1.53e-15 71.6
MS.gene028314.t1 AT5G51870 46.377 69 37 0 1 69 11 79 2.21e-15 71.2
MS.gene028314.t1 AT5G51860 31.737 167 89 6 1 164 1 145 3.64e-15 70.5
MS.gene028314.t1 AT5G51860 31.737 167 89 6 1 164 1 145 4.28e-15 70.1
MS.gene028314.t1 AT5G20240 37.037 108 61 3 1 104 1 105 4.48e-15 68.9
MS.gene028314.t1 AT5G65070 47.458 59 31 0 1 59 1 59 4.97e-15 68.6
MS.gene028314.t1 AT5G65060 49.153 59 30 0 1 59 1 59 5.09e-15 69.7
MS.gene028314.t1 AT3G61120 52.632 57 27 0 1 57 1 57 5.69e-15 70.5
MS.gene028314.t1 AT3G54340 50.000 60 30 0 1 60 1 60 6.52e-15 70.1
MS.gene028314.t1 AT5G62165 42.029 69 40 0 1 69 1 69 8.10e-15 68.6
MS.gene028314.t1 AT5G62165 42.029 69 40 0 1 69 1 69 8.10e-15 68.6
MS.gene028314.t1 AT5G62165 42.029 69 40 0 1 69 1 69 8.10e-15 68.6
MS.gene028314.t1 AT5G62165 42.029 69 40 0 1 69 1 69 8.45e-15 68.2
MS.gene028314.t1 AT5G62165 42.029 69 40 0 1 69 1 69 8.45e-15 68.2
MS.gene028314.t1 AT5G62165 42.029 69 40 0 1 69 1 69 8.45e-15 68.2
MS.gene028314.t1 AT5G62165 42.029 69 40 0 1 69 1 69 8.45e-15 68.2
MS.gene028314.t1 AT5G62165 42.029 69 40 0 1 69 1 69 8.45e-15 68.2
MS.gene028314.t1 AT5G65070 47.458 59 31 0 1 59 1 59 1.02e-14 68.9
MS.gene028314.t1 AT5G20240 43.662 71 38 1 1 69 1 71 1.23e-14 68.9
MS.gene028314.t1 AT1G26310 45.714 70 37 1 1 69 1 70 1.33e-14 69.7
MS.gene028314.t1 AT3G30260 33.962 106 66 2 1 105 1 103 1.37e-14 69.7
MS.gene028314.t1 AT5G65060 49.153 59 30 0 1 59 1 59 1.58e-14 68.6
MS.gene028314.t1 AT4G11880 49.180 61 31 0 1 61 1 61 1.59e-14 68.2
MS.gene028314.t1 AT4G11880 49.180 61 31 0 1 61 1 61 1.59e-14 68.2
MS.gene028314.t1 AT5G65060 49.153 59 30 0 1 59 1 59 1.80e-14 68.2
MS.gene028314.t1 AT4G37435 30.282 142 89 5 1 139 1 135 2.01e-14 68.2
MS.gene028314.t1 AT5G62165 42.029 69 40 0 1 69 1 69 2.52e-14 68.2
MS.gene028314.t1 AT5G62165 42.029 69 40 0 1 69 1 69 2.52e-14 68.2
MS.gene028314.t1 AT5G62165 42.029 69 40 0 1 69 1 69 2.62e-14 68.2
MS.gene028314.t1 AT5G62165 42.029 69 40 0 1 69 1 69 2.62e-14 68.2
MS.gene028314.t1 AT5G62165 42.029 69 40 0 1 69 1 69 2.62e-14 68.2
MS.gene028314.t1 AT5G62165 42.029 69 40 0 1 69 1 69 2.81e-14 65.9
MS.gene028314.t1 AT5G65050 49.153 59 30 0 1 59 1 59 3.12e-14 67.4
MS.gene028314.t1 AT5G65070 47.458 59 31 0 1 59 1 59 3.43e-14 68.2
MS.gene028314.t1 AT5G65070 47.458 59 31 0 1 59 1 59 3.60e-14 67.8
MS.gene028314.t1 AT5G65070 47.458 59 31 0 1 59 1 59 4.49e-14 67.8
MS.gene028314.t1 AT2G22630 43.478 69 39 0 1 69 1 69 5.06e-14 67.8
MS.gene028314.t1 AT2G22630 43.478 69 39 0 1 69 1 69 5.06e-14 67.8
MS.gene028314.t1 AT5G60910 33.094 139 76 5 1 135 1 126 6.86e-14 67.4
MS.gene028314.t1 AT4G11880 49.180 61 31 0 1 61 1 61 6.95e-14 67.4
MS.gene028314.t1 AT4G11880 49.180 61 31 0 1 61 1 61 6.95e-14 67.4
MS.gene028314.t1 AT1G69120 32.231 121 78 3 1 120 1 118 7.54e-14 67.4
MS.gene028314.t1 AT2G03710 47.761 67 34 1 1 66 1 67 8.97e-14 66.2
MS.gene028314.t1 AT3G02310 47.541 61 32 0 1 61 1 61 1.31e-13 66.2
MS.gene028314.t1 AT1G69120 32.500 120 77 3 1 119 1 117 1.35e-13 67.0
MS.gene028314.t1 AT1G72350 51.724 58 28 0 2 59 43 100 1.66e-13 66.2
MS.gene028314.t1 AT5G65050 49.153 59 30 0 1 59 1 59 1.71e-13 65.9
MS.gene028314.t1 AT5G15800 47.541 61 32 0 1 61 1 61 1.79e-13 66.6
MS.gene028314.t1 AT5G65050 49.153 59 30 0 1 59 1 59 1.99e-13 65.5
MS.gene028314.t1 AT5G65050 49.153 59 30 0 1 59 1 59 2.23e-13 65.1
MS.gene028314.t1 AT5G15800 47.541 61 32 0 1 61 1 61 2.50e-13 66.2
MS.gene028314.t1 AT3G58780 46.774 62 33 0 1 62 16 77 2.60e-13 66.2
MS.gene028314.t1 AT3G58780 29.630 162 107 3 1 162 16 170 3.05e-13 65.9
MS.gene028314.t1 AT3G58780 31.933 119 78 1 1 119 16 131 3.08e-13 66.2
MS.gene028314.t1 AT3G58780 29.630 162 107 3 1 162 22 176 3.09e-13 65.9
MS.gene028314.t1 AT3G02310 47.541 61 32 0 1 61 1 61 3.18e-13 65.9
MS.gene028314.t1 AT2G03710 47.761 67 34 1 1 66 1 67 3.35e-13 65.9
MS.gene028314.t1 AT3G58780 33.628 113 61 2 1 100 16 127 3.35e-13 65.9
MS.gene028314.t1 AT2G03710 47.761 67 34 1 1 66 1 67 3.42e-13 65.9
MS.gene028314.t1 AT2G42830 42.029 69 40 0 1 69 16 84 4.30e-13 65.5
MS.gene028314.t1 AT1G24260 47.541 61 32 0 1 61 1 61 4.38e-13 65.5
MS.gene028314.t1 AT2G42830 42.029 69 40 0 1 69 16 84 4.47e-13 65.5
MS.gene028314.t1 AT1G24260 49.153 59 30 0 1 59 1 59 4.53e-13 65.5
MS.gene028314.t1 AT1G24260 49.153 59 30 0 1 59 1 59 4.53e-13 65.5
MS.gene028314.t1 AT3G04100 31.481 108 69 1 2 104 14 121 7.99e-13 64.3
MS.gene028314.t1 AT1G77980 45.070 71 38 1 1 70 1 71 1.75e-12 64.3
MS.gene028314.t1 AT1G22130 38.144 97 59 1 1 96 1 97 2.07e-12 64.3
MS.gene028314.t1 AT4G18960 41.176 68 40 0 2 69 18 85 2.52e-12 63.5
MS.gene028314.t1 AT1G54760 38.356 73 44 1 32 103 18 90 2.01e-11 59.7
MS.gene028314.t1 AT5G65080 31.298 131 89 1 1 131 8 137 2.14e-11 60.1
MS.gene028314.t1 AT5G65080 47.273 55 29 0 1 55 8 62 2.73e-11 60.1
MS.gene028314.t1 AT2G26320 35.211 71 44 1 1 69 17 87 3.96e-11 57.4
MS.gene028314.t1 AT1G77080 52.542 59 28 0 1 59 1 59 4.17e-11 58.9
MS.gene028314.t1 AT1G77080 52.542 59 28 0 1 59 1 59 5.76e-11 58.5

Find 30 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACAAAGGTAAAGCCTTTA+TGG 0.178976 4.2:-17591439 MS.gene028314:CDS
GGTGGTAAACCATTTGCTTT+TGG 0.303762 4.2:-17591609 MS.gene028314:CDS
AATGCTAGAGTTGGTATAGT+TGG 0.376951 4.2:-17591642 MS.gene028314:CDS
TCTAAATCAATGATGTGTAT+AGG 0.399337 4.2:+17591309 None:intergenic
GTGACTAATGAGTACCTTCA+TGG 0.419147 4.2:-17591567 MS.gene028314:CDS
TGATTTAGATTGTAAAAGAA+AGG 0.419907 4.2:-17591295 MS.gene028314:CDS
TTAGTCACAGCTTCGAAAGT+TGG 0.428007 4.2:+17591582 None:intergenic
GTCAACTTCATCCAACTCTT+CGG 0.435885 4.2:+17591458 None:intergenic
ATAATTAGTTTCAATAACAT+TGG 0.469629 4.2:+17591266 None:intergenic
GTTGGAGAACCAAAAGCAAA+TGG 0.473738 4.2:+17591600 None:intergenic
AAGATCAGTAGGTACCATAA+AGG 0.480919 4.2:+17591425 None:intergenic
ATTCTTTGTAATGCTAGAGT+TGG 0.486006 4.2:-17591651 MS.gene028314:CDS
GATTTAGATTGTAAAAGAAA+GGG 0.486822 4.2:-17591294 MS.gene028314:CDS
GGTATAGTTGGATTTACCCC+TGG 0.497794 4.2:-17591630 MS.gene028314:CDS
TGTGTATAGGTTTCTTTGCA+AGG 0.525836 4.2:+17591322 None:intergenic
ATTCACCTTGTTCTCCATGA+AGG 0.540934 4.2:+17591553 None:intergenic
AGAATTAGTGGATAACAAGA+AGG 0.557557 4.2:-17591736 MS.gene028314:CDS
TAGTGGATAACAAGAAGGCT+AGG 0.564393 4.2:-17591731 MS.gene028314:CDS
TCTTCTAAGTCAAGATCAGT+AGG 0.576234 4.2:+17591414 None:intergenic
GAGTGATTTGACCGAAGAGT+TGG 0.587637 4.2:-17591469 MS.gene028314:CDS
AAAGCAAATGGTTTACCACC+AGG 0.590273 4.2:+17591612 None:intergenic
GAGTACCTTCATGGAGAACA+AGG 0.593782 4.2:-17591558 MS.gene028314:CDS
AGAAGAACTTCTAAAAGCGA+AGG 0.608771 4.2:-17591397 MS.gene028314:CDS
AGCAAATGGTTTACCACCAG+GGG 0.628921 4.2:+17591614 None:intergenic
GCATCATTGAAAGAGCTCCA+TGG 0.644750 4.2:-17591375 MS.gene028314:CDS
AAGCAAATGGTTTACCACCA+GGG 0.655343 4.2:+17591613 None:intergenic
ATAGTTGGATTTACCCCTGG+TGG 0.655734 4.2:-17591627 MS.gene028314:CDS
AGAGCTCCATGGTAACATTG+AGG 0.658337 4.2:-17591364 MS.gene028314:CDS
ATGCTGCCTCAATGTTACCA+TGG 0.672251 4.2:+17591358 None:intergenic
GAGTTGGATGAAGTTGACAA+AGG 0.684442 4.2:-17591453 MS.gene028314:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTAGTTTCAATAACAT+TGG + chr4.2:17591759-17591778 None:intergenic 15.0%
!! AAAAATAGAGATAGAATTAG+TGG - chr4.2:17591274-17591293 MS.gene028314:CDS 20.0%
!! GATTTAGATTGTAAAAGAAA+GGG - chr4.2:17591728-17591747 MS.gene028314:CDS 20.0%
!! TGATTTAGATTGTAAAAGAA+AGG - chr4.2:17591727-17591746 MS.gene028314:CDS 20.0%
! TCTAAATCAATGATGTGTAT+AGG + chr4.2:17591716-17591735 None:intergenic 25.0%
ATTCTTTGTAATGCTAGAGT+TGG - chr4.2:17591371-17591390 MS.gene028314:CDS 30.0%
! ACATTTTCAAAGAGAAGAAG+AGG - chr4.2:17591320-17591339 MS.gene028314:CDS 30.0%
! AGAATTAGTGGATAACAAGA+AGG - chr4.2:17591286-17591305 MS.gene028314:CDS 30.0%
!! AAGAAGAGGTCTTTTTAAGA+AGG - chr4.2:17591334-17591353 MS.gene028314:CDS 30.0%
AAGATCAGTAGGTACCATAA+AGG + chr4.2:17591600-17591619 None:intergenic 35.0%
TCTTCTAAGTCAAGATCAGT+AGG + chr4.2:17591611-17591630 None:intergenic 35.0%
TGACAAAGGTAAAGCCTTTA+TGG - chr4.2:17591583-17591602 MS.gene028314:CDS 35.0%
TGTGTATAGGTTTCTTTGCA+AGG + chr4.2:17591703-17591722 None:intergenic 35.0%
! AGAAGAACTTCTAAAAGCGA+AGG - chr4.2:17591625-17591644 MS.gene028314:CDS 35.0%
!! AATGCTAGAGTTGGTATAGT+TGG - chr4.2:17591380-17591399 MS.gene028314:CDS 35.0%
AAAGCAAATGGTTTACCACC+AGG + chr4.2:17591413-17591432 None:intergenic 40.0%
AAGCAAATGGTTTACCACCA+GGG + chr4.2:17591412-17591431 None:intergenic 40.0%
ATTCACCTTGTTCTCCATGA+AGG + chr4.2:17591472-17591491 None:intergenic 40.0%
GAGTTGGATGAAGTTGACAA+AGG - chr4.2:17591569-17591588 MS.gene028314:CDS 40.0%
GTCAACTTCATCCAACTCTT+CGG + chr4.2:17591567-17591586 None:intergenic 40.0%
GTGACTAATGAGTACCTTCA+TGG - chr4.2:17591455-17591474 MS.gene028314:CDS 40.0%
GTTGGAGAACCAAAAGCAAA+TGG + chr4.2:17591425-17591444 None:intergenic 40.0%
TTAGTCACAGCTTCGAAAGT+TGG + chr4.2:17591443-17591462 None:intergenic 40.0%
! GGTGGTAAACCATTTGCTTT+TGG - chr4.2:17591413-17591432 MS.gene028314:CDS 40.0%
! TAGTGGATAACAAGAAGGCT+AGG - chr4.2:17591291-17591310 MS.gene028314:CDS 40.0%
AGAGCTCCATGGTAACATTG+AGG - chr4.2:17591658-17591677 MS.gene028314:CDS 45.0%
AGCAAATGGTTTACCACCAG+GGG + chr4.2:17591411-17591430 None:intergenic 45.0%
ATAGTTGGATTTACCCCTGG+TGG - chr4.2:17591395-17591414 MS.gene028314:CDS 45.0%
ATGCTGCCTCAATGTTACCA+TGG + chr4.2:17591667-17591686 None:intergenic 45.0%
GAGTACCTTCATGGAGAACA+AGG - chr4.2:17591464-17591483 MS.gene028314:CDS 45.0%
GAGTGATTTGACCGAAGAGT+TGG - chr4.2:17591553-17591572 MS.gene028314:CDS 45.0%
GCATCATTGAAAGAGCTCCA+TGG - chr4.2:17591647-17591666 MS.gene028314:CDS 45.0%
GGTATAGTTGGATTTACCCC+TGG - chr4.2:17591392-17591411 MS.gene028314:CDS 45.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 17591263 17591781 17591263 ID=MS.gene028314
chr4.2 mRNA 17591263 17591781 17591263 ID=MS.gene028314.t1;Parent=MS.gene028314
chr4.2 exon 17591263 17591781 17591263 ID=MS.gene028314.t1.exon1;Parent=MS.gene028314.t1
chr4.2 CDS 17591263 17591781 17591263 ID=cds.MS.gene028314.t1;Parent=MS.gene028314.t1
Gene Sequence

>MS.gene028314

ATGGGTCGTAAAAAAATAGAGATAGAATTAGTGGATAACAAGAAGGCTAGGAATGTCACATTTTCAAAGAGAAGAAGAGGTCTTTTTAAGAAGGCAAGCGATTTATCAATTCTTTGTAATGCTAGAGTTGGTATAGTTGGATTTACCCCTGGTGGTAAACCATTTGCTTTTGGTTCTCCAACTTTCGAAGCTGTGACTAATGAGTACCTTCATGGAGAACAAGGTGAATCATCGAGACAGAATGTTAAGTCTTCTCAAAATCAAAACATCAACAAGCTCAACAAAGAACTGAGTGATTTGACCGAAGAGTTGGATGAAGTTGACAAAGGTAAAGCCTTTATGGTACCTACTGATCTTGACTTAGAAGAACTTCTAAAAGCGAAGGCATCATTGAAAGAGCTCCATGGTAACATTGAGGCAGCATCATCTTTGCTTCTCCTTGCAAAGAAACCTATACACATCATTGATTTAGATTGTAAAAGAAAGGGAAAATCCAATGTTATTGAAACTAATTATTAA
Protein sequence

>MS.gene028314.t1

MGRKKIEIELVDNKKARNVTFSKRRRGLFKKASDLSILCNARVGIVGFTPGGKPFAFGSPTFEAVTNEYLHGEQGESSRQNVKSSQNQNINKLNKELSDLTEELDEVDKGKAFMVPTDLDLEELLKAKASLKELHGNIEAASSLLLLAKKPIHIIDLDCKRKGKSNVIETNY