AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028317

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028317.t1 MTR_4g094622 97.368 152 3 1 1 151 1 152 1.85e-104 295
MS.gene028317.t1 MTR_8g073345 46.067 89 48 0 59 147 33 121 1.05e-23 90.5
MS.gene028317.t1 MTR_8g073345 46.067 89 48 0 59 147 33 121 1.62e-23 89.7
MS.gene028317.t1 MTR_3g115580 47.674 86 45 0 53 138 35 120 3.08e-23 89.4
MS.gene028317.t1 MTR_7g068280 51.316 76 37 0 59 134 47 122 1.88e-22 87.8
MS.gene028317.t1 MTR_1g115220 48.315 89 45 1 59 146 98 186 1.09e-21 87.0
MS.gene028317.t1 MTR_7g082820 44.444 81 44 1 55 135 5 84 2.32e-17 73.9
MS.gene028317.t1 MTR_5g024180 38.272 81 50 0 52 132 25 105 4.54e-15 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028317.t1 AT2G34450 55.921 152 65 2 1 151 1 151 4.92e-51 160
MS.gene028317.t1 AT2G34450 55.921 152 65 2 1 151 1 151 4.92e-51 160
MS.gene028317.t1 AT2G34450 55.921 152 65 2 1 151 1 151 4.92e-51 160
MS.gene028317.t1 AT2G34450 55.556 153 65 3 1 151 1 152 3.30e-49 155
MS.gene028317.t1 AT2G17560 43.478 115 61 1 41 151 13 127 8.43e-24 90.5
MS.gene028317.t1 AT2G17560 43.478 115 61 1 41 151 13 127 8.97e-24 90.5
MS.gene028317.t1 AT2G17560 43.478 115 61 1 41 151 13 127 8.97e-24 90.5
MS.gene028317.t1 AT3G51880 44.554 101 56 0 50 150 40 140 1.60e-23 90.5
MS.gene028317.t1 AT3G51880 44.118 102 57 0 50 151 40 141 2.06e-23 90.5
MS.gene028317.t1 AT3G51880 44.118 102 57 0 50 151 40 141 2.06e-23 90.5
MS.gene028317.t1 AT3G51880 44.118 102 57 0 50 151 40 141 2.06e-23 90.5
MS.gene028317.t1 AT3G51880 44.118 102 57 0 50 151 40 141 2.26e-23 90.9
MS.gene028317.t1 AT1G20693 46.667 90 46 1 59 148 34 121 5.03e-22 86.3
MS.gene028317.t1 AT1G20693 46.667 90 46 1 59 148 34 121 5.16e-22 86.3
MS.gene028317.t1 AT4G35570 44.318 88 49 0 59 146 30 117 5.58e-22 85.5
MS.gene028317.t1 AT4G35570 44.318 88 49 0 59 146 30 117 5.58e-22 85.5
MS.gene028317.t1 AT1G20693 46.667 90 46 1 59 148 34 121 1.19e-21 85.1
MS.gene028317.t1 AT1G20696 45.556 90 47 1 59 148 31 118 7.78e-21 82.8
MS.gene028317.t1 AT1G20696 44.156 77 43 0 59 135 31 107 4.96e-18 75.9
MS.gene028317.t1 AT1G20696 44.156 77 43 0 59 135 31 107 5.22e-18 75.5
MS.gene028317.t1 AT5G23420 47.059 68 35 1 60 127 112 178 6.33e-13 64.3
MS.gene028317.t1 AT1G20696 43.243 74 40 1 75 148 5 76 4.62e-12 59.3
MS.gene028317.t1 AT1G20696 43.243 74 40 1 75 148 5 76 1.25e-11 58.2

Find 19 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCTTCGGCCTCAACCTC+CGG 0.331912 4.2:-17625071 MS.gene028317:CDS
AACAGAGTATAACAAGAAAA+TGG 0.350035 4.2:-17622321 MS.gene028317:intron
TCAAAAGAACGCTTCTTCTT+CGG 0.408803 4.2:-17625084 MS.gene028317:CDS
TCACGCATTGACTTAACATC+TGG 0.420177 4.2:+17623693 None:intergenic
CTTCTTCGGCCTCAACCTCC+GGG 0.476651 4.2:-17625070 MS.gene028317:intron
GAAAGGGTACCCGGAGGTTG+AGG 0.506068 4.2:+17625061 None:intergenic
GCAAGGCATGTGGAGAGAAG+TGG 0.571530 4.2:-17623399 MS.gene028317:CDS
CGTGTTCATACAGATTGGCA+AGG 0.572075 4.2:-17623416 MS.gene028317:intron
AAGAAGTAGAAGAAAGCAGT+GGG 0.576065 4.2:+17624415 None:intergenic
GTTGGCGATCAAATCGAATG+AGG 0.587280 4.2:-17624539 MS.gene028317:CDS
GAAGTAGAAGAAAGCAGTGG+GGG 0.593069 4.2:+17624417 None:intergenic
TCTGATGCTTTGCAGGAAAG+CGG 0.595152 4.2:-17622226 MS.gene028317:intron
ATACAGATTGGCAAGGCATG+TGG 0.602187 4.2:-17623409 MS.gene028317:intron
CGAATATGAAGAAACTGATG+AGG 0.603289 4.2:-17622203 MS.gene028317:CDS
TTGGCGATCAAATCGAATGA+GGG 0.633911 4.2:-17624538 MS.gene028317:CDS
CAAGAAGTAGAAGAAAGCAG+TGG 0.644048 4.2:+17624414 None:intergenic
AAGTAGAAGAAAGCAGTGGG+GGG 0.691336 4.2:+17624418 None:intergenic
AGGGAATGAAGAGATCGTTG+CGG 0.720017 4.2:-17624519 MS.gene028317:CDS
AGAAGTAGAAGAAAGCAGTG+GGG 0.730489 4.2:+17624416 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATACATTATTAATTTAACA+AGG - chr4.2:17623018-17623037 MS.gene028317:intron 10.0%
!! ATAAATTAAGTTTCAAAAAT+TGG - chr4.2:17624029-17624048 MS.gene028317:intron 10.0%
!! ATAAATTATGTTTCAAAAAT+TGG - chr4.2:17624104-17624123 MS.gene028317:intron 10.0%
!! ATACATTATTAATTTAACAA+GGG - chr4.2:17623019-17623038 MS.gene028317:intron 10.0%
!! ATTCAAGTAAAAAAAATTAT+TGG - chr4.2:17622495-17622514 MS.gene028317:intron 10.0%
!! TTGTTATATTTGAAAAAAAT+TGG - chr4.2:17624259-17624278 MS.gene028317:intron 10.0%
!! AATAAAATCAATTAACGAAA+AGG - chr4.2:17624617-17624636 MS.gene028317:intron 15.0%
!! ACTACTATTTACAATATATT+TGG - chr4.2:17623423-17623442 MS.gene028317:intron 15.0%
!! ATAAAATCAATTAACGAAAA+GGG - chr4.2:17624618-17624637 MS.gene028317:intron 15.0%
!! ATCAAATTGAACATAATATA+TGG + chr4.2:17624834-17624853 None:intergenic 15.0%
!!! AATTGATTTTTAGAAGATTT+AGG - chr4.2:17622466-17622485 MS.gene028317:intron 15.0%
!!! ATTGATTTTTAGAAGATTTA+GGG - chr4.2:17622467-17622486 MS.gene028317:intron 15.0%
!!! CGATTTTTATGTTTAATTAT+TGG - chr4.2:17624465-17624484 MS.gene028317:CDS 15.0%
!!! TAATTTTTTTTACTTGAATG+AGG + chr4.2:17622494-17622513 None:intergenic 15.0%
!! AAAATTGAATAAAATGATGG+CGG - chr4.2:17622587-17622606 MS.gene028317:intron 20.0%
!! AATAAGCAGAAAACAATTTA+TGG + chr4.2:17622358-17622377 None:intergenic 20.0%
!! TAAAATCAATTAACGAAAAG+GGG - chr4.2:17624619-17624638 MS.gene028317:intron 20.0%
!! TCTCAAAAACATAAAATAAC+TGG + chr4.2:17623779-17623798 None:intergenic 20.0%
!! TTATGTTTAATTATTGGTGT+TGG - chr4.2:17624471-17624490 MS.gene028317:CDS 20.0%
!! TTTGAAAAAAATTGGAAAGT+TGG - chr4.2:17624267-17624286 MS.gene028317:intron 20.0%
!!! AAGTTTTTTTTCATTCTGAA+GGG - chr4.2:17623548-17623567 MS.gene028317:intron 20.0%
!!! ACAATTGCTTCTTTTTTATA+TGG - chr4.2:17623487-17623506 MS.gene028317:intron 20.0%
!!! ATAACTGGCAATTTTTAAAA+TGG + chr4.2:17623764-17623783 None:intergenic 20.0%
!!! CCATAAAAATTTAGAACAAA+TGG + chr4.2:17622998-17623017 None:intergenic 20.0%
!!! CCATCATTTTATTCAATTTT+GGG + chr4.2:17622587-17622606 None:intergenic 20.0%
!!! CCATTTGTTCTAAATTTTTA+TGG - chr4.2:17622995-17623014 MS.gene028317:intron 20.0%
!!! TTTTATTTTCATAAGCTCTT+CGG - chr4.2:17622439-17622458 MS.gene028317:intron 20.0%
! AACAGAGTATAACAAGAAAA+TGG - chr4.2:17624975-17624994 MS.gene028317:intron 25.0%
! AATAAGACATAGTAGAAACA+AGG + chr4.2:17623386-17623405 None:intergenic 25.0%
! ATAGTAGTATAATTGAACAG+AGG + chr4.2:17623411-17623430 None:intergenic 25.0%
! ATTATAAAGACACGTAAACT+AGG + chr4.2:17624587-17624606 None:intergenic 25.0%
! CCCAAAATTGAATAAAATGA+TGG - chr4.2:17622584-17622603 MS.gene028317:intron 25.0%
! CTAACTTTCTTCTATAGTTA+CGG - chr4.2:17622308-17622327 MS.gene028317:intron 25.0%
! TAACAAAAATTGTATCTGAC+AGG - chr4.2:17624894-17624913 MS.gene028317:intron 25.0%
! TACATCATAAACAAAGAACA+AGG + chr4.2:17622927-17622946 None:intergenic 25.0%
! TAGACGAAATATTAATACGA+CGG + chr4.2:17623643-17623662 None:intergenic 25.0%
! TAGTAATGAATCAGTAATTG+TGG + chr4.2:17623092-17623111 None:intergenic 25.0%
! TTACAATATATTTGGCATTG+TGG - chr4.2:17623431-17623450 MS.gene028317:intron 25.0%
! TTCAAAATTACTGTGTTTAC+TGG - chr4.2:17623525-17623544 MS.gene028317:intron 25.0%
!! AATTCAGGTTAAATATGCAA+GGG + chr4.2:17623963-17623982 None:intergenic 25.0%
!! ACCATTTGTTGATTAACAAT+TGG - chr4.2:17623263-17623282 MS.gene028317:intron 25.0%
!! ATAAACAATGATGATGATGA+TGG + chr4.2:17623814-17623833 None:intergenic 25.0%
!! GAATTTTGTCAAAACTGTTT+AGG - chr4.2:17624133-17624152 MS.gene028317:intron 25.0%
!! GCCATCATTTTATTCAATTT+TGG + chr4.2:17622588-17622607 None:intergenic 25.0%
!! TAACCTGAATTGTGTTATTA+TGG - chr4.2:17623972-17623991 MS.gene028317:intron 25.0%
!!! AAACTAGTTCTGTATTGATT+TGG + chr4.2:17624763-17624782 None:intergenic 25.0%
!!! AACTGTTTAGGTTTTTAATG+TGG - chr4.2:17624145-17624164 MS.gene028317:intron 25.0%
!!! ATCAGTTTTTTGACTCATTT+AGG - chr4.2:17624422-17624441 MS.gene028317:CDS 25.0%
!!! GAAGTTTTTTTTCATTCTGA+AGG - chr4.2:17623547-17623566 MS.gene028317:intron 25.0%
!!! TATTTTACGGATTTTGACTT+AGG - chr4.2:17623199-17623218 MS.gene028317:intron 25.0%
!!! TCAGTTTTTTGACTCATTTA+GGG - chr4.2:17624423-17624442 MS.gene028317:CDS 25.0%
!!! TTTATTTTCATAAGCTCTTC+GGG - chr4.2:17622440-17622459 MS.gene028317:intron 25.0%
ATCATAAACAAAGAACAAGG+AGG + chr4.2:17622924-17622943 None:intergenic 30.0%
CAACCATAATAACACAATTC+AGG + chr4.2:17623978-17623997 None:intergenic 30.0%
GCACATTGGTCATAATAAAA+GGG - chr4.2:17622530-17622549 MS.gene028317:intron 30.0%
GCCAATTGTTAATCAACAAA+TGG + chr4.2:17623267-17623286 None:intergenic 30.0%
TAAACATGTTTGAAGATGAG+TGG + chr4.2:17623319-17623338 None:intergenic 30.0%
TAACCTATCTGTCATATTCA+TGG - chr4.2:17624998-17625017 MS.gene028317:intron 30.0%
TGCACATTGGTCATAATAAA+AGG - chr4.2:17622529-17622548 MS.gene028317:intron 30.0%
TGTAAAACAAAGTTGCTTCA+GGG + chr4.2:17623689-17623708 None:intergenic 30.0%
TTATGACCAATGTGCAATTT+AGG + chr4.2:17622525-17622544 None:intergenic 30.0%
TTGTAAAACAAAGTTGCTTC+AGG + chr4.2:17623690-17623709 None:intergenic 30.0%
! ATGTTTCAAAAATTGGCTCA+GGG - chr4.2:17624111-17624130 MS.gene028317:intron 30.0%
! ATTTGGAATAAATAGCAGCA+CGG + chr4.2:17624746-17624765 None:intergenic 30.0%
! CAATTCAGGTTAAATATGCA+AGG + chr4.2:17623964-17623983 None:intergenic 30.0%
! TATGTTTCAAAAATTGGCTC+AGG - chr4.2:17624110-17624129 MS.gene028317:intron 30.0%
!! AAGATCACTTTGGAATTTCT+AGG - chr4.2:17624314-17624333 MS.gene028317:intron 30.0%
!! TGGATGGTATTTTAGAAAAC+TGG + chr4.2:17624651-17624670 None:intergenic 30.0%
!! TTTCTAAAATACCATCCATG+TGG - chr4.2:17624653-17624672 MS.gene028317:intron 30.0%
!!! TTTTTTTTCATTCTGAAGGG+AGG - chr4.2:17623551-17623570 MS.gene028317:intron 30.0%
AAGAAGTAGAAGAAAGCAGT+GGG + chr4.2:17622884-17622903 None:intergenic 35.0%
AAGAAGTCATGATCAAGACA+TGG + chr4.2:17624192-17624211 None:intergenic 35.0%
CAAGTACTACAAACCTACAA+CGG + chr4.2:17624514-17624533 None:intergenic 35.0%
CCATTGTCAGGAATTTAAAC+AGG + chr4.2:17624799-17624818 None:intergenic 35.0%
CCTGTTTAAATTCCTGACAA+TGG - chr4.2:17624796-17624815 MS.gene028317:intron 35.0%
CGAATATGAAGAAACTGATG+AGG - chr4.2:17625093-17625112 MS.gene028317:CDS 35.0%
GACATAGTAGAAACAAGGAT+AGG + chr4.2:17623381-17623400 None:intergenic 35.0%
GCAAAAAAGAAACACCACTT+TGG - chr4.2:17624858-17624877 MS.gene028317:intron 35.0%
GTAAAACAAAGTTGCTTCAG+GGG + chr4.2:17623688-17623707 None:intergenic 35.0%
TAGCCATGAATATGACAGAT+AGG + chr4.2:17625004-17625023 None:intergenic 35.0%
TCAAAAGAACGCTTCTTCTT+CGG - chr4.2:17622212-17622231 MS.gene028317:CDS 35.0%
TGGAAAGTTGGAATTCATTG+CGG - chr4.2:17624279-17624298 MS.gene028317:intron 35.0%
TTGTCAGTTGTGATGTTTGA+CGG - chr4.2:17624063-17624082 MS.gene028317:intron 35.0%
TTTCTTCTATAGTTACGGTC+TGG - chr4.2:17622313-17622332 MS.gene028317:intron 35.0%
! AATGCTGCATTGAGTTTTTC+AGG - chr4.2:17622726-17622745 MS.gene028317:intron 35.0%
! GTGGAAAACAATGACTTTTG+AGG - chr4.2:17623916-17623935 MS.gene028317:intron 35.0%
! TATACTATCGCGTAAGTTGA+TGG - chr4.2:17623165-17623184 MS.gene028317:intron 35.0%
!! ATACTATCGCGTAAGTTGAT+GGG - chr4.2:17623166-17623185 MS.gene028317:intron 35.0%
!! CTTATTTTCACAAGCTCTTC+AGG - chr4.2:17622372-17622391 MS.gene028317:CDS 35.0%
!! TTTGTTTTCTGATGCTTTGC+AGG - chr4.2:17625063-17625082 MS.gene028317:intron 35.0%
!!! AGGTAGAGTGTATTTTGACT+TGG - chr4.2:17623219-17623238 MS.gene028317:intron 35.0%
!!! GTTCTTTTTTTGCACTGCTT+TGG + chr4.2:17622825-17622844 None:intergenic 35.0%
!!! GTTTTTCAGGAAACTAGTGT+TGG - chr4.2:17622739-17622758 MS.gene028317:intron 35.0%
!!! TAGAGTGTATTTTGACTTGG+AGG - chr4.2:17623222-17623241 MS.gene028317:intron 35.0%
!!! TGTATTTTGACTTGGAGGAA+TGG - chr4.2:17623227-17623246 MS.gene028317:intron 35.0%
AACATAGAGCTCAACCAAAG+TGG + chr4.2:17624875-17624894 None:intergenic 40.0%
AAGGGACCAACAATGCAATT+AGG - chr4.2:17622548-17622567 MS.gene028317:intron 40.0%
ACGCGATAGTATAACTCCAA+AGG + chr4.2:17623158-17623177 None:intergenic 40.0%
ACTGATCTGTGTGTTTCCTT+TGG - chr4.2:17623139-17623158 MS.gene028317:intron 40.0%
AGAAGTAGAAGAAAGCAGTG+GGG + chr4.2:17622883-17622902 None:intergenic 40.0%
AGCAGAACTGAAGATCACTT+TGG - chr4.2:17624304-17624323 MS.gene028317:intron 40.0%
AGGAGAGTAGAGAAAGTGAA+AGG + chr4.2:17622255-17622274 None:intergenic 40.0%
ATACTTGACACTCCATTGTC+AGG + chr4.2:17624811-17624830 None:intergenic 40.0%
ATCCATGTGGAAACTTACCT+GGG - chr4.2:17624666-17624685 MS.gene028317:intron 40.0%
CAAGAAGTAGAAGAAAGCAG+TGG + chr4.2:17622885-17622904 None:intergenic 40.0%
CAATCTGTATGAACACGGAA+AGG + chr4.2:17623877-17623896 None:intergenic 40.0%
CTTGCCAATCTGTATGAACA+CGG + chr4.2:17623882-17623901 None:intergenic 40.0%
CTTTCCGTGTTCATACAGAT+TGG - chr4.2:17623875-17623894 MS.gene028317:intron 40.0%
GGAAGACCTAAATTGCACAT+TGG - chr4.2:17622516-17622535 MS.gene028317:intron 40.0%
GGAGAGTAGAGAAAGTGAAA+GGG + chr4.2:17622254-17622273 None:intergenic 40.0%
TAGCTTCAGGTGTTAGTAGT+GGG - chr4.2:17622646-17622665 MS.gene028317:intron 40.0%
TGCTCAACTCTTGAACTTGA+GGG + chr4.2:17623295-17623314 None:intergenic 40.0%
TTGCTCAACTCTTGAACTTG+AGG + chr4.2:17623296-17623315 None:intergenic 40.0%
TTGGCGATCAAATCGAATGA+GGG - chr4.2:17622758-17622777 MS.gene028317:intron 40.0%
! ACAAGGGTTTTGCTGCATTA+AGG - chr4.2:17623035-17623054 MS.gene028317:intron 40.0%
! ACTCTCCTTTTCGTTCACAA+GGG - chr4.2:17622267-17622286 MS.gene028317:intron 40.0%
! CACGTAAACTAGGTGCTATA+AGG + chr4.2:17624577-17624596 None:intergenic 40.0%
! CTAGCGAACTGACTCTTATT+TGG - chr4.2:17625021-17625040 MS.gene028317:intron 40.0%
! CTTTTCCCTTGTGAACGAAA+AGG + chr4.2:17622275-17622294 None:intergenic 40.0%
! GGGGGTTCTGTTGTATTTTA+CGG - chr4.2:17623186-17623205 MS.gene028317:intron 40.0%
! TACTCTCCTTTTCGTTCACA+AGG - chr4.2:17622266-17622285 MS.gene028317:intron 40.0%
!! CAATTTTGGGTCTAAGACCA+AGG + chr4.2:17622574-17622593 None:intergenic 40.0%
!! CATTCTGAAGGGAGGATTTT+CGG - chr4.2:17623559-17623578 MS.gene028317:intron 40.0%
!! TACTATCGCGTAAGTTGATG+GGG - chr4.2:17623167-17623186 MS.gene028317:intron 40.0%
!! TCACGCATTGACTTAACATC+TGG + chr4.2:17623606-17623625 None:intergenic 40.0%
AAGTAGAAGAAAGCAGTGGG+GGG + chr4.2:17622881-17622900 None:intergenic 45.0%
AGCTTCAGGTGTTAGTAGTG+GGG - chr4.2:17622647-17622666 MS.gene028317:intron 45.0%
AGGGAATGAAGAGATCGTTG+CGG - chr4.2:17622777-17622796 MS.gene028317:intron 45.0%
ATACAGATTGGCAAGGCATG+TGG - chr4.2:17623887-17623906 MS.gene028317:intron 45.0%
CAGGTAAGTTTCCACATGGA+TGG + chr4.2:17624667-17624686 None:intergenic 45.0%
CATCCATGTGGAAACTTACC+TGG - chr4.2:17624665-17624684 MS.gene028317:intron 45.0%
CCAACAATGCAATTAGGCCT+TGG - chr4.2:17622554-17622573 MS.gene028317:intron 45.0%
CCAAGGCCTAATTGCATTGT+TGG + chr4.2:17622557-17622576 None:intergenic 45.0%
CGTGTTCATACAGATTGGCA+AGG - chr4.2:17623880-17623899 MS.gene028317:intron 45.0%
CTACTAACACCTGAAGCTAG+AGG + chr4.2:17622645-17622664 None:intergenic 45.0%
CTAGCTTCAGGTGTTAGTAG+TGG - chr4.2:17622645-17622664 MS.gene028317:intron 45.0%
GAAGTAGAAGAAAGCAGTGG+GGG + chr4.2:17622882-17622901 None:intergenic 45.0%
GCTCAACTCTTGAACTTGAG+GGG + chr4.2:17623294-17623313 None:intergenic 45.0%
GTTGGCGATCAAATCGAATG+AGG - chr4.2:17622757-17622776 MS.gene028317:intron 45.0%
TCTGATGCTTTGCAGGAAAG+CGG - chr4.2:17625070-17625089 MS.gene028317:intron 45.0%
TCTGTATGAACACGGAAAGG+AGG + chr4.2:17623874-17623893 None:intergenic 45.0%
TGGAAACTTACCTGGGACTA+AGG - chr4.2:17624673-17624692 MS.gene028317:intron 45.0%
TGTGAGTTTCAACCGTGTCT+AGG - chr4.2:17622613-17622632 MS.gene028317:intron 45.0%
! AGCTTCTTCTTGATAGCAGC+AGG + chr4.2:17624709-17624728 None:intergenic 45.0%
! GTTAACACGAGTACCGTTGT+AGG - chr4.2:17624498-17624517 MS.gene028317:CDS 45.0%
! TAGAGGTGTAGACCTAGACA+CGG + chr4.2:17622628-17622647 None:intergenic 45.0%
!! ACTATCGCGTAAGTTGATGG+GGG - chr4.2:17623168-17623187 MS.gene028317:intron 45.0%
!! AGAGAAAGTGAAAGGGTACC+CGG + chr4.2:17622247-17622266 None:intergenic 45.0%
!! AGCGTTCTTTTGAGAGTGAG+AGG + chr4.2:17622205-17622224 None:intergenic 45.0%
AAAGCAGTGGGGGGTTTCTT+CGG + chr4.2:17622872-17622891 None:intergenic 50.0%
AACTGATGATCATGCCAGCC+TGG + chr4.2:17624409-17624428 None:intergenic 50.0%
AGTTGCAGAACTGTCTCCTC+TGG - chr4.2:17624375-17624394 MS.gene028317:intron 50.0%
GTCCCAGGTAAGTTTCCACA+TGG + chr4.2:17624671-17624690 None:intergenic 50.0%
TCTCCTCTGGTTGGAATTCC+AGG - chr4.2:17624388-17624407 MS.gene028317:intron 50.0%
! AGGTCTACACCTCTAGCTTC+AGG - chr4.2:17622633-17622652 MS.gene028317:intron 50.0%
CAGCCTGGAATTCCAACCAG+AGG + chr4.2:17624394-17624413 None:intergenic 55.0%
CTCTGGTTGGAATTCCAGGC+TGG - chr4.2:17624392-17624411 MS.gene028317:intron 55.0%
CTTTCACGTGCCTTAGTCCC+AGG + chr4.2:17624686-17624705 None:intergenic 55.0%
GCAAGGCATGTGGAGAGAAG+TGG - chr4.2:17623897-17623916 MS.gene028317:intron 55.0%
GCAGAACTGTCTCCTCTGGT+TGG - chr4.2:17624379-17624398 MS.gene028317:intron 55.0%
TCTTCTTCGGCCTCAACCTC+CGG - chr4.2:17622225-17622244 MS.gene028317:intron 55.0%
!! GAAAGTGAAAGGGTACCCGG+AGG + chr4.2:17622244-17622263 None:intergenic 55.0%
CTTCTTCGGCCTCAACCTCC+GGG - chr4.2:17622226-17622245 MS.gene028317:intron 60.0%
GACTTGGAGGAATGGCGCAG+AGG - chr4.2:17623235-17623254 MS.gene028317:intron 60.0%
TTGGAGGAATGGCGCAGAGG+AGG - chr4.2:17623238-17623257 MS.gene028317:intron 60.0%
!! GAAAGGGTACCCGGAGGTTG+AGG + chr4.2:17622238-17622257 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 17622183 17625135 17622183 ID=MS.gene028317
chr4.2 mRNA 17622183 17625135 17622183 ID=MS.gene028317.t1;Parent=MS.gene028317
chr4.2 exon 17625071 17625135 17625071 ID=MS.gene028317.t1.exon1;Parent=MS.gene028317.t1
chr4.2 CDS 17625071 17625135 17625071 ID=cds.MS.gene028317.t1;Parent=MS.gene028317.t1
chr4.2 exon 17624415 17624570 17624415 ID=MS.gene028317.t1.exon2;Parent=MS.gene028317.t1
chr4.2 CDS 17624415 17624570 17624415 ID=cds.MS.gene028317.t1;Parent=MS.gene028317.t1
chr4.2 exon 17623692 17623749 17623692 ID=MS.gene028317.t1.exon3;Parent=MS.gene028317.t1
chr4.2 CDS 17623692 17623749 17623692 ID=cds.MS.gene028317.t1;Parent=MS.gene028317.t1
chr4.2 exon 17623381 17623425 17623381 ID=MS.gene028317.t1.exon4;Parent=MS.gene028317.t1
chr4.2 CDS 17623381 17623425 17623381 ID=cds.MS.gene028317.t1;Parent=MS.gene028317.t1
chr4.2 exon 17622322 17622402 17622322 ID=MS.gene028317.t1.exon5;Parent=MS.gene028317.t1
chr4.2 CDS 17622322 17622402 17622322 ID=cds.MS.gene028317.t1;Parent=MS.gene028317.t1
chr4.2 exon 17622183 17622233 17622183 ID=MS.gene028317.t1.exon6;Parent=MS.gene028317.t1
chr4.2 CDS 17622183 17622233 17622183 ID=cds.MS.gene028317.t1;Parent=MS.gene028317.t1
Gene Sequence

>MS.gene028317

ATGGCGAAAAAAGCTAAGGCCTCTCACTCTCAAAAGAACGCTTCTTCTTCGGCCTCAACCTCCGGGTACCCTTTCACTTTCTCTACTCTCCTTTTCGTTCACAAGGGAAAAGCTATATTTTAAAACTAACTTTCTTCTATAGTTACGGTCTGGAGACAATTTATGACATCGTCCATAAATTGTTTTCTGCTTATTTTCACAAGCTCTTCAGGATAACTTATGAAAACAGCTTGCAGCTTATATGAAAACAAATTGATTTTATTTTCATAAGCTCTTCGGGATAAATTGATTTTTAGAAGATTTAGGGACCTCATTCAAGTAAAAAAAATTATTGGAAGACCTAAATTGCACATTGGTCATAATAAAAGGGACCAACAATGCAATTAGGCCTTGGTCTTAGACCCAAAATTGAATAAAATGATGGCGGCATTGTGAGTTTCAACCGTGTCTAGGTCTACACCTCTAGCTTCAGGTGTTAGTAGTGGGGAAATTTGATTTATTACATTTAACTATGTGAAGAAATAGTTTTGCTTAACTAAACACAATGCTGCATTGAGTTTTTCAGGAAACTAGTGTTGGCGATCAAATCGAATGAGGGAATGAAGAGATCGTTGCGGCAGACTAAGTCAAGAGACTTAACCAAAGCAGTGCAAAAAAAGAACAAGCAAAAGATTGATGCTAAGAAGCCGAAGAAACCCCCCACTGCTTTCTTCTACTTCTTGTAAAACTTCTTTTATTCCTCCTTGTTCTTTGTTTATGATGTATAATTCTTATTTTTAATATGTGTTAGTATAAACTACTCTTTGCATTTTCCATTTGTTCTAAATTTTTATGGAATACATTATTAATTTAACAAGGGTTTTGCTGCATTAAGGTTTATTTGTCTGTATATATGAAAAAGTAGCACCACAATTACTGATTCATTACTAGAATGAATAAGTATAGTTAATGTTCATACTGATCTGTGTGTTTCCTTTGGAGTTATACTATCGCGTAAGTTGATGGGGGTTCTGTTGTATTTTACGGATTTTGACTTAGGTAGAGTGTATTTTGACTTGGAGGAATGGCGCAGAGGAGGTAACCATTTGTTGATTAACAATTGGCATTACCCCTCAAGTTCAAGAGTTGAGCAACCACTCATCTTCAAACATGTTTATCATTAGTCATTTAAAAACAATTTTCTTCTGATAGTTTTCCTATCCTTGTTTCTACTATGTCTTATTGTCCTCTGTTCAATTATACTACTATTTACAATATATTTGGCATTGTGGTAATTAATCTAGCACAGTGTCTGTGTATAACAAACAATTGCTTCTTTTTTATATGGAAAATCTTATAATGCTTCAAAATTACTGTGTTTACTGGAAGTTTTTTTTCATTCTGAAGGGAGGATTTTCGGAAAGAGTTTAAAGATCAGAATCCAGATGTTAAGTCAATGCGTGATGTATGTATAGAATCCGTCGTATTAATATTTCGTCTAATTTATTTTATACTTTTAATTTCCCCTGAAGCAACTTTGTTTTACAATTGATCGTTTAGAATTTTCAAAATTATATAAACAACACAGATGTCTAACATCCATTTTAAAAATTGCCAGTTATTTTATGTTTTTGAGACTGAAAATATCACCATCATCATCATCATTGTTTATCATCATCATGCAAAGTAATATTTTTCTGTTTTGTTTTCCTCCTTTCCGTGTTCATACAGATTGGCAAGGCATGTGGAGAGAAGTGGAAAACAATGACTTTTGAGGTTAGTTTTTCTTTGCTCTTCTCCCTTGCATATTTAACCTGAATTGTGTTATTATGGTTGTGATGTTTGACTGCTGAAAACTGAGTGAATCATAAATTAAGTTTCAAAAATTGGCTCAGAGAATTTTGTCAGTTGTGATGTTTGACGGCTAAAAACTGAGTGAATCATAAATTATGTTTCAAAAATTGGCTCAGGGAATTTTGTCAAAACTGTTTAGGTTTTTAATGTGGCTGAATGTTTGAACTTGATTGCCATGTCTTGATCATGACTTCTTCGAATATCAATGAATTAAATTTTGAACAATTCTTGAAGAACAAGTTTTTGTTATATTTGAAAAAAATTGGAAAGTTGGAATTCATTGCGGCAAGCAGAACTGAAGATCACTTTGGAATTTCTAGGTTATGTACAATTTTGCAATTCAGCTACTGTAATGCAAAAGTTGCAGAACTGTCTCCTCTGGTTGGAATTCCAGGCTGGCATGATCATCAGTTTTTTGACTCATTTAGGGACAGAGTGTTTTTTTTCTTCGATTTTTATGTTTAATTATTGGTGTTGGTAGCGTTAACACGAGTACCGTTGTAGGTTTGTAGTACTTGTATTGACTGCACAAAATGTTGATTCAGTCATTTCATCAAACCTTATAGCACCTAGTTTACGTGTCTTTATAATCATCTTTAAAAATAAAATCAATTAACGAAAAGGGGTTCAAACCAGTTTTCTAAAATACCATCCATGTGGAAACTTACCTGGGACTAAGGCACGTGAAAGCCTGCTGCTATCAAGAAGAAGCTTCTGCTTGTGCTAACCGTGCTGCTATTTATTCCAAATCAATACAGAACTAGTTTTTCAAGTTTGTTTCCTGTTTAAATTCCTGACAATGGAGTGTCAAGTATCCATATATTATGTTCAATTTGATCTGAGCAAAAAAGAAACACCACTTTGGTTGAGCTCTATGTTAACAAAAATTGTATCTGACAGGAGAAAGTTCAATACTATGATATAGCAACAGAGAAACGTGCAGAATTTGATAGAGCAACAACAGAGTATAACAAGAAAATGGTAACCTATCTGTCATATTCATGGCTAGCGAACTGACTCTTATTTGGCATGTTACTAAATATACAATTTGTTTTCTGATGCTTTGCAGGAAAGCGGCGAATATGAAGAAACTGATGAGGAGTCAGAGTATGACGAGTAA
Protein sequence

>MS.gene028317.t1

MAKKAKASHSQKNASSSASTSGKLVLAIKSNEGMKRSLRQTKSRDLTKAVQKKNKQKIDAKKPKKPPTAFFYFLEDFRKEFKDQNPDVKSMRDIGKACGEKWKTMTFEEKVQYYDIATEKRAEFDRATTEYNKKMESGEYEETDEESEYDE