AlfalfaGEDB | MS.gene028544

M. sativa cultivar XinJiangDaYe / MS.gene028544

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028544.t1	AES90509.2	91.3	426	32	3	36	461	174	594	2.40E-223	784.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028544.t1	Q9ASS4	38.4	346	177	8	148	459	274	617	2.6e-56	221.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028544.t1	G7JSZ5	91.3	426	32	3	36	461	174	594	1.8e-223	784.6

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028544.t1	MTR_4g092530	88.937	461	45	4	1	461	140	594	0.0	833
MS.gene028544.t1	MTR_4g092520	71.552	464	84	6	1	461	153	571	0.0	649
MS.gene028544.t1	MTR_4g092550	72.959	392	101	2	62	453	46	432	0.0	572
MS.gene028544.t1	MTR_7g080810	37.908	459	215	11	2	430	162	580	1.35e-87	280
MS.gene028544.t1	MTR_8g010180	36.424	453	220	13	2	424	164	578	7.22e-76	249
MS.gene028544.t1	MTR_4g092560	45.645	287	130	6	159	430	21	296	3.10e-74	238
MS.gene028544.t1	MTR_4g092570	91.964	112	9	0	340	451	17	128	1.29e-68	215
MS.gene028544.t1	MTR_5g057660	43.450	313	148	8	159	456	15	313	7.52e-67	217
MS.gene028544.t1	MTR_3g095100	32.292	480	260	15	1	443	709	1160	3.51e-58	207
MS.gene028544.t1	MTR_5g057345	43.262	282	131	8	190	456	1	268	4.48e-58	192

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028544.t1	AT5G48380	34.483	493	252	12	2	459	161	617	2.55e-81	263
MS.gene028544.t1	AT3G13380	32.599	454	250	15	2	423	699	1128	7.67e-56	200
MS.gene028544.t1	AT1G55610	31.373	459	251	15	2	423	699	1130	8.29e-55	197
MS.gene028544.t1	AT1G55610	31.373	459	251	15	2	423	699	1130	8.29e-55	197
MS.gene028544.t1	AT3G28450	30.753	465	263	12	2	442	163	592	1.06e-52	187
MS.gene028544.t1	AT2G01950	29.459	499	270	14	2	444	671	1143	2.01e-52	191
MS.gene028544.t1	AT4G39400	31.352	488	267	16	2	448	714	1174	1.80e-51	187
MS.gene028544.t1	AT5G07280	31.223	458	263	14	1	430	759	1192	1.54e-49	182
MS.gene028544.t1	AT1G27190	30.853	457	254	11	2	430	157	579	3.96e-48	174
MS.gene028544.t1	AT1G69990	28.698	453	263	11	2	430	150	566	7.26e-47	171

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Find 94 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGGTGAACACACTCAC+AGG	0.574145	4.1:-20637347	None:intergenic
AAAATATAGTTCCCATGCTT+GGG	0.399198	4.1:+20636816	MS.gene028544:CDS
AAATTTGGCCTCTCCAAAAT+TGG	0.218462	4.1:-20637083	None:intergenic
AACCTGCTCCTTTCCTTCAA+TGG	0.349416	4.1:-20636843	None:intergenic
AACGTTAGGAAGGGACCAAA+TGG	0.513438	4.1:+20637415	MS.gene028544:CDS
AACTCGACGATTTCATCTAT+AGG	0.424335	4.1:-20637490	None:intergenic
AAGAAGGATGTCTACGACTT+TGG	0.316828	4.1:+20637160	MS.gene028544:CDS
AAGATGGGAACGATGTACCA+AGG	0.699980	4.1:+20636686	MS.gene028544:CDS
AAGATTGACATGGCATCAAA+GGG	0.554516	4.1:+20636934	MS.gene028544:CDS
AATATGAGCAACGTTAGGAA+GGG	0.657864	4.1:+20637406	MS.gene028544:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAATATAGTTCCCATGCT+TGG	+	chr4.1:20636815-20636834	MS.gene028544:CDS	30.0%
AAAATATAGTTCCCATGCTT+GGG	+	chr4.1:20636816-20636835	MS.gene028544:CDS	30.0%
CTAAACAAAGCCATAGAATT+GGG	+	chr4.1:20636345-20636364	MS.gene028544:CDS	30.0%
CTTAGATGATCATCAATGTT+GGG	-	chr4.1:20637115-20637134	None:intergenic	30.0%
CTTATGACTTCACTTTCTAA+AGG	-	chr4.1:20636917-20636936	None:intergenic	30.0%
AAATTTGGCCTCTCCAAAAT+TGG	-	chr4.1:20637086-20637105	None:intergenic	35.0%
AACTCGACGATTTCATCTAT+AGG	-	chr4.1:20637493-20637512	None:intergenic	35.0%
AAGATTGACATGGCATCAAA+GGG	+	chr4.1:20636934-20636953	MS.gene028544:CDS	35.0%
AATATGAGCAACGTTAGGAA+GGG	+	chr4.1:20637406-20637425	MS.gene028544:CDS	35.0%
AATATGCTTGCCCAATTCTA+TGG	-	chr4.1:20636358-20636377	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	20636012	20637516	20636012	ID=MS.gene028544
chr4.1	mRNA	20636012	20637516	20636012	ID=MS.gene028544.t1;Parent=MS.gene028544
chr4.1	exon	20636012	20636467	20636012	ID=MS.gene028544.t1.exon1;Parent=MS.gene028544.t1
chr4.1	CDS	20636012	20636467	20636012	ID=cds.MS.gene028544.t1;Parent=MS.gene028544.t1
chr4.1	exon	20636587	20637516	20636587	ID=MS.gene028544.t1.exon2;Parent=MS.gene028544.t1
chr4.1	CDS	20636587	20637516	20636587	ID=cds.MS.gene028544.t1;Parent=MS.gene028544.t1

Gene Sequence

>MS.gene028544

ATGTTAAGCGGTGAAATTCCACCACAATTTGCCGGACTTGCGAGGCTTAAAACAATTTCTTTTTCCAACAATCAATTGTATGGGCCAGTGCCAAATTTCAAAACAGGATTAGTAGATGTTGCTAGCTATGCTAACAATAGTGGACTTTGTGGAGCGCCTTTGAGACCTTGCTCCTTGACACGTGATAAGCACGGCGATTTCCATCAATCGTTCAAGAGTGGTCTTATTGTTGGTTATGTTTTTTCACTAATTTTTTCTGTTACGCTCACTTATATCTTCTACTCCAAATGTGCACATTGGGTGCATCAATCGAAAAAGAAGAAAAACAATCACCTAAACAAAGCCATAGAATTGGGCAAGCATATTATCTCCATCACCAGCAGGAGAACACAGATGGTAGCTGATCAGATGCACCAATTGTTACATTCGTGGATTGTACACAAAGAGACCAAAGAGGTACTCAATCTCTTCTTTAAATAGTTCTATCTTTTTAGACACTCCTTGTTTTGAATTGTTTTTTAGGTTAATCAGTGTATATTGTCTGTTGTAAAATATAATTACACATGCATCCACAGATCTCTATACTAATGGAGAGACTGACTTCAACAATATGGTTGGAAGAACTGCGTGACGCGACCGATTGTTTTGCTGTGGACAATGCCATTGGGGTAGGAAAGATGGGAACGATGTACCAAGGATTTCTGCCTAATGGTCAATTACTAGCTGTTAAGAGACTATTTGACTCTCGCCTCTTTAAAAGGCAGTTCCTTTTAGAAACAGCAATTTTGTGCAAGTACAGACACAAAAATATAGTTCCCATGCTTGGGTTTTCCATTGAAGGAAAGGAGCAGGTTTTGGCATATGCATACATGTCAAATGGAAGGCTTTCCAAATGGTTGCATCCTTTAGAAAGTGAAGTCATAAGATTGACATGGCATCAAAGGGTTAACATTGCACTTGGAATAGCAAGAGGATTATCATGGCTGCATCATACATGCGATTTGGGCATAGTGCATTTTAGCATATGTTCCGAATGCATATTGCTAGATGAGAATTTTGAACCAAAAATATCCAATTTTGGAGAGGCCAAATTTATGAATCCCAACATTGATGATCATCTAAGCACAATGTTTAAAGTGAATGACGGGAAGAAGGATGTCTACGACTTTGGAAGTGTGCTTTTTGAATTGATGACAGGGAAAACATATAATGAATTGTCGCGTTCATCTGACACTACTAATCTATGTGGTAACCCTTTGAGTTTTTATAATGTCATTGATAAATCTTTGACCGAGGAAGGACTTGAAAATGAAGTATGTACTCTCATCAAGATTGCCTGTGAGTGTGTTCACCCTTTTCCAGACCAAAGGCCAACAATGCTCGAAGTTTACAACAATATGAGCAACGTTAGGAAGGGACCAAATGGTTCTGGTGATGATTCTGACACATTAAGGAGATTAGAATATGCTTCTTCTATTCCTATAGATGAAATCGTCGAGTTTTAA

Protein sequence

>MS.gene028544.t1

MLSGEIPPQFAGLARLKTISFSNNQLYGPVPNFKTGLVDVASYANNSGLCGAPLRPCSLTRDKHGDFHQSFKSGLIVGYVFSLIFSVTLTYIFYSKCAHWVHQSKKKKNNHLNKAIELGKHIISITSRRTQMVADQMHQLLHSWIVHKETKEISILMERLTSTIWLEELRDATDCFAVDNAIGVGKMGTMYQGFLPNGQLLAVKRLFDSRLFKRQFLLETAILCKYRHKNIVPMLGFSIEGKEQVLAYAYMSNGRLSKWLHPLESEVIRLTWHQRVNIALGIARGLSWLHHTCDLGIVHFSICSECILLDENFEPKISNFGEAKFMNPNIDDHLSTMFKVNDGKKDVYDFGSVLFELMTGKTYNELSRSSDTTNLCGNPLSFYNVIDKSLTEEGLENEVCTLIKIACECVHPFPDQRPTMLEVYNNMSNVRKGPNGSGDDSDTLRRLEYASSIPIDEIVEF