AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028699

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028699.t1 MTR_2g035730 70.492 122 7 2 42 163 1 93 3.84e-48 156
MS.gene028699.t1 MTR_1g023660 51.613 124 60 0 172 295 217 340 9.08e-43 150
MS.gene028699.t1 MTR_8g099780 53.846 104 48 0 192 295 209 312 5.65e-40 142
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028699.t1 AT2G01930 55.556 297 101 10 16 295 1 283 4.23e-97 287
MS.gene028699.t1 AT2G01930 55.556 297 101 10 16 295 1 283 4.23e-97 287
MS.gene028699.t1 AT1G14685 51.178 297 110 10 16 295 1 279 1.07e-87 263
MS.gene028699.t1 AT1G14685 51.178 297 110 10 16 295 1 279 1.07e-87 263
MS.gene028699.t1 AT1G14685 51.178 297 110 10 16 295 1 279 1.07e-87 263
MS.gene028699.t1 AT1G14685 51.178 297 110 10 16 295 1 279 1.07e-87 263
MS.gene028699.t1 AT1G68120 47.079 291 121 9 16 295 1 269 4.78e-74 228
MS.gene028699.t1 AT2G35550 47.159 176 81 2 123 295 60 226 9.20e-50 164
MS.gene028699.t1 AT2G35550 47.159 176 81 2 123 295 60 226 9.20e-50 164
MS.gene028699.t1 AT2G35550 47.159 176 81 2 123 295 60 226 9.20e-50 164
MS.gene028699.t1 AT2G35550 47.159 176 81 2 123 295 105 271 1.54e-49 165
MS.gene028699.t1 AT5G42520 56.190 105 45 1 192 295 178 282 1.56e-39 140
MS.gene028699.t1 AT5G42520 56.190 105 45 1 192 295 234 338 3.14e-39 140
MS.gene028699.t1 AT5G42520 56.190 105 45 1 192 295 238 342 3.92e-39 140
MS.gene028699.t1 AT2G21240 52.885 104 49 0 192 295 193 296 6.04e-39 139
MS.gene028699.t1 AT2G21240 52.885 104 49 0 192 295 193 296 6.04e-39 139
MS.gene028699.t1 AT4G38910 51.485 101 49 0 195 295 158 258 7.32e-38 135
MS.gene028699.t1 AT4G38910 51.485 101 49 0 195 295 183 283 1.31e-37 135

Find 88 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGGAAGAACAACATAATT+TGG 0.132141 2.1:+8442144 None:intergenic
CTTCGGCTTCCTGGGCTTCT+TGG 0.176884 2.1:+8441972 None:intergenic
GCTATCATCCTTCGGCTTCC+TGG 0.179821 2.1:+8441963 None:intergenic
GGAATTGAAATGGACATTTC+TGG 0.204966 2.1:-8441883 MS.gene028699:CDS
GCCACGAGATTGTGCTGTTT+CGG 0.312141 2.1:-8442247 MS.gene028699:CDS
TTCCTGGGCTTCTTGGCTTT+AGG 0.348093 2.1:+8441979 None:intergenic
CTTTGCCGATGAGCATAAAA+AGG 0.358237 2.1:-8441753 MS.gene028699:CDS
AGCGAAGAAAAGGAAGTCTA+AGG 0.361093 2.1:-8442016 MS.gene028699:CDS
TTAACTATGTGAGGGATAAC+TGG 0.365336 2.1:-8442209 MS.gene028699:CDS
TTCTCATTTCTTGATGCATC+AGG 0.370349 2.1:+8442084 None:intergenic
GAGAGTGATTGTGATGCTTC+TGG 0.377872 2.1:+8442126 None:intergenic
ATCACAATCACTCTCCTTGC+CGG 0.381788 2.1:-8442118 MS.gene028699:CDS
TGAAATCAATGGAATTGAAA+TGG 0.399496 2.1:-8441893 MS.gene028699:CDS
AGGCGTGGTGCAAGGATAGC+TGG 0.400781 2.1:-8441733 MS.gene028699:CDS
GCATCAGGCTGCGGTATAAC+CGG 0.400929 2.1:+8442099 None:intergenic
GGGTTACTATGAACCCTTCA+AGG 0.401456 2.1:-8442385 MS.gene028699:CDS
CTATTGATCTGAAGACTCAT+TGG 0.414033 2.1:-8441636 MS.gene028699:CDS
CTATCATCCTTCGGCTTCCT+GGG 0.424580 2.1:+8441964 None:intergenic
GCTATGATAGGAAGTGCAAA+TGG 0.431333 2.1:-8442285 MS.gene028699:CDS
TCAAGTGCCAAAGTCTTCTT+CGG 0.439736 2.1:+8441913 None:intergenic
ATCGCTGGGGCATTGGAGGC+TGG 0.440357 2.1:-8441813 MS.gene028699:CDS
GTTCCAGTTTGTTCTTGCAC+TGG 0.441790 2.1:-8441853 MS.gene028699:CDS
TTCCAGTTTGTTCTTGCACT+GGG 0.445134 2.1:-8441852 MS.gene028699:CDS
TTCCCAGTGCAAGAACAAAC+TGG 0.448442 2.1:+8441850 None:intergenic
TCCTCAGCAGTGTTATCGCT+GGG 0.451577 2.1:-8441827 MS.gene028699:CDS
TATTGATAAACCTATGTCTC+GGG 0.452192 2.1:+8442181 None:intergenic
TGTTATCGCTGGGGCATTGG+AGG 0.456365 2.1:-8441817 MS.gene028699:CDS
TTTGCACTTCCTATCATAGC+TGG 0.459364 2.1:+8442288 None:intergenic
CAGTGTTATCGCTGGGGCAT+TGG 0.459602 2.1:-8441820 MS.gene028699:CDS
TCTGATAGTGACAAACTTGT+TGG 0.463051 2.1:+8441600 None:intergenic
CATCAAGTAATTCCTTCTCT+CGG 0.464965 2.1:-8443865 MS.gene028699:CDS
GGGTTCATAGTAACCCCAAT+TGG 0.466238 2.1:+8442392 None:intergenic
ATCCTCAGCAGTGTTATCGC+TGG 0.466509 2.1:-8441828 MS.gene028699:CDS
TGAAGAATTGAGTGTTAAGA+AGG 0.467743 2.1:-8442049 MS.gene028699:CDS
ATGTGCTGAATATGCCCAAT+TGG 0.468228 2.1:-8442407 MS.gene028699:CDS
GGTCGTGATCCAGCTATGAT+AGG 0.473752 2.1:-8442297 MS.gene028699:CDS
ATCAAGTAATTCCTTCTCTC+GGG 0.478528 2.1:-8443864 MS.gene028699:CDS
AAGGTGGCAAAGCGAAGAAA+AGG 0.482686 2.1:-8442026 MS.gene028699:CDS
TTTCTTGATGCATCAGGCTG+CGG 0.490246 2.1:+8442090 None:intergenic
AGGATAAATTGACACATTTG+TGG 0.492724 2.1:+8441774 None:intergenic
GTGCAAGAACAAACTGGAAC+TGG 0.493288 2.1:+8441856 None:intergenic
ATCTCGTGGCTGAAAGGGCA+AGG 0.493413 2.1:+8442260 None:intergenic
AGCACAATCTCGTGGCTGAA+AGG 0.494131 2.1:+8442254 None:intergenic
TTATCCCTCACATAGTTAAA+CGG 0.497017 2.1:+8442213 None:intergenic
CTCCTAAAGCCAAGAAGCCC+AGG 0.497299 2.1:-8441981 MS.gene028699:CDS
GAAACCACCGAAGAAGACTT+TGG 0.499299 2.1:-8441920 MS.gene028699:CDS
ACTTTGGCACTTGAAATCAA+TGG 0.500357 2.1:-8441904 MS.gene028699:CDS
GTTTCACGATCATTCATGGC+AGG 0.511266 2.1:+8442336 None:intergenic
GGTTCATAGTAACCCCAATT+GGG 0.512276 2.1:+8442393 None:intergenic
AATTCCTTCTCTCGGGAACC+AGG 0.513249 2.1:-8443857 MS.gene028699:intron
GAAACACTGCTATCATCCTT+CGG 0.515134 2.1:+8441955 None:intergenic
TGTGCTGAATATGCCCAATT+GGG 0.516384 2.1:-8442406 MS.gene028699:CDS
GCACAATCTCGTGGCTGAAA+GGG 0.516986 2.1:+8442255 None:intergenic
TATTGATCTGAAGACTCATT+GGG 0.517362 2.1:-8441635 MS.gene028699:CDS
GGGCTTCTTGGCTTTAGGAG+TGG 0.523313 2.1:+8441984 None:intergenic
ATATTGATAAACCTATGTCT+CGG 0.524517 2.1:+8442180 None:intergenic
GTTACTATGAACCCTTCAAG+GGG 0.524731 2.1:-8442383 MS.gene028699:CDS
GAATTGAGTGTTAAGAAGGA+AGG 0.526010 2.1:-8442045 MS.gene028699:CDS
GGCGTGGTGCAAGGATAGCT+GGG 0.537643 2.1:-8441732 MS.gene028699:CDS
GGTTACTATGAACCCTTCAA+GGG 0.540273 2.1:-8442384 MS.gene028699:CDS
ACTGGATGACCCCGAGACAT+AGG 0.551501 2.1:-8442191 MS.gene028699:CDS
CAAGAAGCCCAGGAAGCCGA+AGG 0.555978 2.1:-8441971 MS.gene028699:CDS
TTGAGTGTTAAGAAGGAAGG+TGG 0.573084 2.1:-8442042 MS.gene028699:CDS
CTAACCTGGTTCCCGAGAGA+AGG 0.573473 2.1:+8443853 None:intergenic
TACTATGAACCCTTCAAGGG+GGG 0.578585 2.1:-8442381 MS.gene028699:CDS
CTATGATAGGAAGTGCAAAT+GGG 0.579589 2.1:-8442284 MS.gene028699:CDS
CTACTCCGTTTAACTATGTG+AGG 0.583386 2.1:-8442218 MS.gene028699:CDS
TTACTATGAACCCTTCAAGG+GGG 0.587367 2.1:-8442382 MS.gene028699:CDS
GCGAAGAAAAGGAAGTCTAA+GGG 0.589646 2.1:-8442015 MS.gene028699:CDS
AGTGCCAAAGTCTTCTTCGG+TGG 0.589946 2.1:+8441916 None:intergenic
ATCATAGCTGGATCACGACC+AGG 0.590957 2.1:+8442300 None:intergenic
ACTATGAACCCTTCAAGGGG+GGG 0.591165 2.1:-8442380 MS.gene028699:CDS
CTGGGAGGAAAATGAGCCGA+GGG 0.596548 2.1:-8441714 MS.gene028699:CDS
TCTCGTGGCTGAAAGGGCAA+GGG 0.616671 2.1:+8442261 None:intergenic
AAGACTCATTGGGCTAGACA+TGG 0.622877 2.1:-8441625 MS.gene028699:CDS
AGGCTGCGGTATAACCGGCA+AGG 0.642668 2.1:+8442104 None:intergenic
TCCGAAACAGCACAATCTCG+TGG 0.644189 2.1:+8442246 None:intergenic
TACTCCGTTTAACTATGTGA+GGG 0.653092 2.1:-8442217 MS.gene028699:CDS
CCCCAGCGATAACACTGCTG+AGG 0.654198 2.1:+8441826 None:intergenic
GATCATTCATGGCAGGCATG+AGG 0.655695 2.1:+8442343 None:intergenic
TGCATCAAGAAATGAGAATG+CGG 0.661175 2.1:-8442079 MS.gene028699:CDS
GGACCTTCTTAAAAGCCCCT+CGG 0.664989 2.1:+8441698 None:intergenic
ATTGATAAACCTATGTCTCG+GGG 0.666123 2.1:+8442182 None:intergenic
GCTGGGAGGAAAATGAGCCG+AGG 0.673676 2.1:-8441715 MS.gene028699:CDS
CCTCAGCAGTGTTATCGCTG+GGG 0.692437 2.1:-8441826 MS.gene028699:CDS
GTGCTGAATATGCCCAATTG+GGG 0.693408 2.1:-8442405 MS.gene028699:CDS
TGGGAGGAAAATGAGCCGAG+GGG 0.729606 2.1:-8441713 MS.gene028699:CDS
GTGGTGCAAGGATAGCTGGG+AGG 0.770772 2.1:-8441729 MS.gene028699:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAAATAAAAATCGAAAA+TGG + chr2.1:8442789-8442808 None:intergenic 10.0%
!! ATTAAATAAAAATCGAAAAT+GGG + chr2.1:8442788-8442807 None:intergenic 10.0%
!!! TTTGTTAAATGAAATTTTAT+AGG - chr2.1:8443005-8443024 MS.gene028699:intron 10.0%
!! AAAAATGAATTAAAATAAGG+AGG - chr2.1:8442194-8442213 MS.gene028699:CDS 15.0%
!! AACAAAAAATATTTATAAGG+AGG + chr2.1:8442489-8442508 None:intergenic 15.0%
!! CAGAACAAAAAATATTTATA+AGG + chr2.1:8442492-8442511 None:intergenic 15.0%
!! CTGTTCAAAAAAAATAATTT+CGG - chr2.1:8442592-8442611 MS.gene028699:intron 15.0%
!! TTCTGTTATGTTTATTTATT+TGG - chr2.1:8442909-8442928 MS.gene028699:intron 15.0%
!! AAAAAATATAGCAAAGTCAA+AGG + chr2.1:8442162-8442181 None:intergenic 20.0%
!! AAAGAGCGAAAAAAAAAAAT+TGG + chr2.1:8442019-8442038 None:intergenic 20.0%
!! AATCTTATGTGGAAAAAAAT+AGG + chr2.1:8441867-8441886 None:intergenic 20.0%
!! AGAATTATGTTATGTATGAA+TGG + chr2.1:8442255-8442274 None:intergenic 20.0%
!! GAATTATGTTATGTATGAAT+GGG + chr2.1:8442254-8442273 None:intergenic 20.0%
!!! AATACCATTTTTAAGCAAAA+AGG + chr2.1:8442716-8442735 None:intergenic 20.0%
!!! ATGTATTCAAATTTTAAAGG+AGG + chr2.1:8442443-8442462 None:intergenic 20.0%
!!! CCTTTAAAATTTGAATACAT+GGG - chr2.1:8442443-8442462 MS.gene028699:intron 20.0%
!!! TCCTTTAAAATTTGAATACA+TGG - chr2.1:8442442-8442461 MS.gene028699:intron 20.0%
!!! TGTATTCAAATTTTAAAGGA+GGG + chr2.1:8442442-8442461 None:intergenic 20.0%
! AAACCAAACTCTTTACTATT+TGG - chr2.1:8441907-8441926 MS.gene028699:CDS 25.0%
! AGATCCGAAAATTGAATTTA+GGG + chr2.1:8442568-8442587 None:intergenic 25.0%
! AGCGAAAAAAAAAAATTGGA+AGG + chr2.1:8442015-8442034 None:intergenic 25.0%
! ATATTGATAAACCTATGTCT+CGG + chr2.1:8443295-8443314 None:intergenic 25.0%
! CAAAAAATGAGAATAGATCT+AGG + chr2.1:8442542-8442561 None:intergenic 25.0%
! CTACAAACATTAAAGATATC+TGG - chr2.1:8441975-8441994 MS.gene028699:CDS 25.0%
! TAGATCCGAAAATTGAATTT+AGG + chr2.1:8442569-8442588 None:intergenic 25.0%
! TATAACAACATTCAGATCTA+GGG - chr2.1:8442652-8442671 MS.gene028699:intron 25.0%
! TATTTATAAGGAGGTTCAAA+AGG + chr2.1:8442480-8442499 None:intergenic 25.0%
! TGAAATCAATGGAATTGAAA+TGG - chr2.1:8443579-8443598 MS.gene028699:intron 25.0%
! TTAGGATTATATGTTATGCT+TGG - chr2.1:8441733-8441752 MS.gene028699:CDS 25.0%
!! AAATTCAATTTTCGGATCTA+CGG - chr2.1:8442569-8442588 MS.gene028699:intron 25.0%
!! CCCATGTATTCAAATTTTAA+AGG + chr2.1:8442446-8442465 None:intergenic 25.0%
!! GAAACCCTAAATTCAATTTT+CGG - chr2.1:8442561-8442580 MS.gene028699:intron 25.0%
!! GTCTTAATTTGAATCTTATG+TGG + chr2.1:8441878-8441897 None:intergenic 25.0%
AGGATAAATTGACACATTTG+TGG + chr2.1:8443701-8443720 None:intergenic 30.0%
ATAACAACATTCAGATCTAG+GGG - chr2.1:8442653-8442672 MS.gene028699:intron 30.0%
ATGGACCAAATTCAAAATGT+GGG - chr2.1:8442626-8442645 MS.gene028699:intron 30.0%
CTATAACAACATTCAGATCT+AGG - chr2.1:8442651-8442670 MS.gene028699:intron 30.0%
GATCCAAATAGTAAAGAGTT+TGG + chr2.1:8441913-8441932 None:intergenic 30.0%
TATTGATAAACCTATGTCTC+GGG + chr2.1:8443294-8443313 None:intergenic 30.0%
TATTGATCTGAAGACTCATT+GGG - chr2.1:8443837-8443856 MS.gene028699:intron 30.0%
TCTGGAAGAACAACATAATT+TGG + chr2.1:8443331-8443350 None:intergenic 30.0%
TGAAGAATTGAGTGTTAAGA+AGG - chr2.1:8443423-8443442 MS.gene028699:intron 30.0%
TTATCCCTCACATAGTTAAA+CGG + chr2.1:8443262-8443281 None:intergenic 30.0%
! AGAATAGATCTAGGTTGAAA+TGG + chr2.1:8442533-8442552 None:intergenic 30.0%
! CCAACCTTTTTGCTTAAAAA+TGG - chr2.1:8442709-8442728 MS.gene028699:intron 30.0%
! TAGTTCCCACATTTTGAATT+TGG + chr2.1:8442634-8442653 None:intergenic 30.0%
!! ATATGCATATGCTTGTTGTT+AGG - chr2.1:8441715-8441734 MS.gene028699:CDS 30.0%
!! CCATTTTTAAGCAAAAAGGT+TGG + chr2.1:8442712-8442731 None:intergenic 30.0%
!! TTTTGTTTCACGATCATTCA+TGG + chr2.1:8443143-8443162 None:intergenic 30.0%
!!! GAAACAAAACCATTTTTTCC+TGG - chr2.1:8443154-8443173 MS.gene028699:intron 30.0%
!!! TTGTTGTTTTGTAGTTGTCA+TGG - chr2.1:8443036-8443055 MS.gene028699:intron 30.0%
AAAAAAAATCGAGTCTCGTC+TGG - chr2.1:8441806-8441825 MS.gene028699:CDS 35.0%
ACTTTGGCACTTGAAATCAA+TGG - chr2.1:8443568-8443587 MS.gene028699:intron 35.0%
AGAGAGAAAACTTACTTGGT+GGG + chr2.1:8442369-8442388 None:intergenic 35.0%
ATCAAGTAATTCCTTCTCTC+GGG - chr2.1:8441608-8441627 MS.gene028699:CDS 35.0%
ATTGATAAACCTATGTCTCG+GGG + chr2.1:8443293-8443312 None:intergenic 35.0%
CATCAAGTAATTCCTTCTCT+CGG - chr2.1:8441607-8441626 MS.gene028699:CDS 35.0%
CATGGACCAAATTCAAAATG+TGG - chr2.1:8442625-8442644 MS.gene028699:intron 35.0%
CTATTGATCTGAAGACTCAT+TGG - chr2.1:8443836-8443855 MS.gene028699:intron 35.0%
CTATTTGGATCAACATGTGT+TGG - chr2.1:8441922-8441941 MS.gene028699:CDS 35.0%
GAATTGAGTGTTAAGAAGGA+AGG - chr2.1:8443427-8443446 MS.gene028699:intron 35.0%
GAGAAAGATAGATATGAGAG+AGG + chr2.1:8442282-8442301 None:intergenic 35.0%
GAGGTTCAAAAGGAGAAAAA+AGG + chr2.1:8442470-8442489 None:intergenic 35.0%
GGAATTGAAATGGACATTTC+TGG - chr2.1:8443589-8443608 MS.gene028699:intron 35.0%
TACTCCGTTTAACTATGTGA+GGG - chr2.1:8443255-8443274 MS.gene028699:intron 35.0%
TGCATCAAGAAATGAGAATG+CGG - chr2.1:8443393-8443412 MS.gene028699:intron 35.0%
TTAACTATGTGAGGGATAAC+TGG - chr2.1:8443263-8443282 MS.gene028699:intron 35.0%
TTCTCATTTCTTGATGCATC+AGG + chr2.1:8443391-8443410 None:intergenic 35.0%
TTGATTCTAGTAGAGGTTTC+AGG - chr2.1:8442091-8442110 MS.gene028699:CDS 35.0%
! AAGAGTTTGGTTTCTCACTA+AGG + chr2.1:8441900-8441919 None:intergenic 35.0%
! ATGTCCACATACTTCTTTTG+AGG - chr2.1:8441665-8441684 MS.gene028699:CDS 35.0%
! CACATTTTGAATTTGGTCCA+TGG + chr2.1:8442627-8442646 None:intergenic 35.0%
! CTATGATAGGAAGTGCAAAT+GGG - chr2.1:8443188-8443207 MS.gene028699:intron 35.0%
! GTAGAAGTTGATTCTAGTAG+AGG - chr2.1:8442084-8442103 MS.gene028699:CDS 35.0%
! TCTGATAGTGACAAACTTGT+TGG + chr2.1:8443875-8443894 None:intergenic 35.0%
! TGTCCACATACTTCTTTTGA+GGG - chr2.1:8441666-8441685 MS.gene028699:CDS 35.0%
! TTACCCTCAAAAGAAGTATG+TGG + chr2.1:8441672-8441691 None:intergenic 35.0%
!! GAGTAATTTGCTGCTTAGTA+AGG - chr2.1:8442965-8442984 MS.gene028699:intron 35.0%
!!! CCTTTTTCAATCTGCTTGTA+GGG - chr2.1:8442833-8442852 MS.gene028699:intron 35.0%
!!! TCCTTTTTCAATCTGCTTGT+AGG - chr2.1:8442832-8442851 MS.gene028699:intron 35.0%
AATTTCGGATCTACGTACCA+TGG - chr2.1:8442607-8442626 MS.gene028699:intron 40.0%
ACACGGAGAGAGAAAAAGAT+TGG + chr2.1:8442399-8442418 None:intergenic 40.0%
AGCGAAGAAAAGGAAGTCTA+AGG - chr2.1:8443456-8443475 MS.gene028699:intron 40.0%
ATCTGCTTGTAGGGTTTATG+TGG - chr2.1:8442842-8442861 MS.gene028699:intron 40.0%
ATGTGCTGAATATGCCCAAT+TGG - chr2.1:8443065-8443084 MS.gene028699:intron 40.0%
CCCTACAAGCAGATTGAAAA+AGG + chr2.1:8442836-8442855 None:intergenic 40.0%
CTACTCCGTTTAACTATGTG+AGG - chr2.1:8443254-8443273 MS.gene028699:intron 40.0%
CTTTGCCGATGAGCATAAAA+AGG - chr2.1:8443719-8443738 MS.gene028699:intron 40.0%
GAAACACTGCTATCATCCTT+CGG + chr2.1:8443520-8443539 None:intergenic 40.0%
GAGAGAAAAAGATTGGAGAG+AGG + chr2.1:8442392-8442411 None:intergenic 40.0%
GAGAGAGAAAACTTACTTGG+TGG + chr2.1:8442370-8442389 None:intergenic 40.0%
GAGGAGAGAGAAAACTTACT+TGG + chr2.1:8442373-8442392 None:intergenic 40.0%
GCGAAGAAAAGGAAGTCTAA+GGG - chr2.1:8443457-8443476 MS.gene028699:intron 40.0%
GGTGGATAATGAAACTCGAA+TGG - chr2.1:8441827-8441846 MS.gene028699:CDS 40.0%
GGTTACTATGAACCCTTCAA+GGG - chr2.1:8443088-8443107 MS.gene028699:intron 40.0%
GGTTCATAGTAACCCCAATT+GGG + chr2.1:8443082-8443101 None:intergenic 40.0%
GTTACTATGAACCCTTCAAG+GGG - chr2.1:8443089-8443108 MS.gene028699:intron 40.0%
GTTATGCTTGGTTGATCAAG+TGG - chr2.1:8441745-8441764 MS.gene028699:CDS 40.0%
TCAAACACCACATCTATGCT+TGG + chr2.1:8442061-8442080 None:intergenic 40.0%
TCAAGTGCCAAAGTCTTCTT+CGG + chr2.1:8443562-8443581 None:intergenic 40.0%
TCAATCTTCTCTCTCCAGAA+GGG - chr2.1:8442321-8442340 MS.gene028699:CDS 40.0%
TGTGCTGAATATGCCCAATT+GGG - chr2.1:8443066-8443085 MS.gene028699:intron 40.0%
TTACTATGAACCCTTCAAGG+GGG - chr2.1:8443090-8443109 MS.gene028699:intron 40.0%
TTCAATCTTCTCTCTCCAGA+AGG - chr2.1:8442320-8442339 MS.gene028699:CDS 40.0%
TTGAGTGTTAAGAAGGAAGG+TGG - chr2.1:8443430-8443449 MS.gene028699:intron 40.0%
TTTAAAGGAGGGTGAGAGAA+GGG + chr2.1:8442431-8442450 None:intergenic 40.0%
TTTGCACTTCCTATCATAGC+TGG + chr2.1:8443187-8443206 None:intergenic 40.0%
! CTTTTTATGCTCATCGGCAA+AGG + chr2.1:8443721-8443740 None:intergenic 40.0%
! GCTATGATAGGAAGTGCAAA+TGG - chr2.1:8443187-8443206 MS.gene028699:intron 40.0%
! TTCCAGTTTGTTCTTGCACT+GGG - chr2.1:8443620-8443639 MS.gene028699:intron 40.0%
!! GTATTTAGGTTTTGCCCTTC+TGG + chr2.1:8442338-8442357 None:intergenic 40.0%
!! TTTTAAAGGAGGGTGAGAGA+AGG + chr2.1:8442432-8442451 None:intergenic 40.0%
AAAAATCGAGTCTCGTCTGG+TGG - chr2.1:8441809-8441828 MS.gene028699:CDS 45.0%
AAGGTGGCAAAGCGAAGAAA+AGG - chr2.1:8443446-8443465 MS.gene028699:intron 45.0%
ATCACAATCACTCTCCTTGC+CGG - chr2.1:8443354-8443373 MS.gene028699:intron 45.0%
CACTCAACCAAGCATAGATG+TGG - chr2.1:8442051-8442070 MS.gene028699:CDS 45.0%
GAAACCACCGAAGAAGACTT+TGG - chr2.1:8443552-8443571 MS.gene028699:intron 45.0%
GAGAGTGATTGTGATGCTTC+TGG + chr2.1:8443349-8443368 None:intergenic 45.0%
GGATCACGACCAGGAAAAAA+TGG + chr2.1:8443166-8443185 None:intergenic 45.0%
GGGTTACTATGAACCCTTCA+AGG - chr2.1:8443087-8443106 MS.gene028699:intron 45.0%
GGGTTCATAGTAACCCCAAT+TGG + chr2.1:8443083-8443102 None:intergenic 45.0%
GTGCAAGAACAAACTGGAAC+TGG + chr2.1:8443619-8443638 None:intergenic 45.0%
GTGCTGAATATGCCCAATTG+GGG - chr2.1:8443067-8443086 MS.gene028699:intron 45.0%
GTTTCACGATCATTCATGGC+AGG + chr2.1:8443139-8443158 None:intergenic 45.0%
TAAGCCAGTGATGACTAACC+TGG + chr2.1:8441636-8441655 None:intergenic 45.0%
TACTATGAACCCTTCAAGGG+GGG - chr2.1:8443091-8443110 MS.gene028699:intron 45.0%
TCTATGCTTGGTTGAGTGAG+TGG + chr2.1:8442049-8442068 None:intergenic 45.0%
TTAAAGGAGGGTGAGAGAAG+GGG + chr2.1:8442430-8442449 None:intergenic 45.0%
TTCCCAGTGCAAGAACAAAC+TGG + chr2.1:8443625-8443644 None:intergenic 45.0%
TTTCTTGATGCATCAGGCTG+CGG + chr2.1:8443385-8443404 None:intergenic 45.0%
! AAGACTCATTGGGCTAGACA+TGG - chr2.1:8443847-8443866 MS.gene028699:intron 45.0%
! ACTTACTTGGTGGGTGCAAA+CGG + chr2.1:8442360-8442379 None:intergenic 45.0%
! CCACGCCTTTTTATGCTCAT+CGG + chr2.1:8443727-8443746 None:intergenic 45.0%
! CTTCAGCAGCAAGTTTTTCC+AGG + chr2.1:8443798-8443817 None:intergenic 45.0%
! GTTCCAGTTTGTTCTTGCAC+TGG - chr2.1:8443619-8443638 MS.gene028699:intron 45.0%
!! CTGGAAAAACTTGCTGCTGA+AGG - chr2.1:8443796-8443815 MS.gene028699:intron 45.0%
!! TAAAAAAATGAATTAAAATA+AGG - chr2.1:8442191-8442210 MS.gene028699:CDS 5.0%
AATTCCTTCTCTCGGGAACC+AGG - chr2.1:8441615-8441634 MS.gene028699:CDS 50.0%
ACTATGAACCCTTCAAGGGG+GGG - chr2.1:8443092-8443111 MS.gene028699:intron 50.0%
AGCACAATCTCGTGGCTGAA+AGG + chr2.1:8443221-8443240 None:intergenic 50.0%
AGTGCCAAAGTCTTCTTCGG+TGG + chr2.1:8443559-8443578 None:intergenic 50.0%
ATCATAGCTGGATCACGACC+AGG + chr2.1:8443175-8443194 None:intergenic 50.0%
ATCCTCAGCAGTGTTATCGC+TGG - chr2.1:8443644-8443663 MS.gene028699:intron 50.0%
CCGATGAGCATAAAAAGGCG+TGG - chr2.1:8443724-8443743 MS.gene028699:intron 50.0%
CTATCATCCTTCGGCTTCCT+GGG + chr2.1:8443511-8443530 None:intergenic 50.0%
GATCATTCATGGCAGGCATG+AGG + chr2.1:8443132-8443151 None:intergenic 50.0%
GCACAATCTCGTGGCTGAAA+GGG + chr2.1:8443220-8443239 None:intergenic 50.0%
GGACCTTCTTAAAAGCCCCT+CGG + chr2.1:8443777-8443796 None:intergenic 50.0%
GGTCGTGATCCAGCTATGAT+AGG - chr2.1:8443175-8443194 MS.gene028699:intron 50.0%
GGTGGGTGCAAACGGTATTT+AGG + chr2.1:8442352-8442371 None:intergenic 50.0%
TCCGAAACAGCACAATCTCG+TGG + chr2.1:8443229-8443248 None:intergenic 50.0%
TCCTCAGCAGTGTTATCGCT+GGG - chr2.1:8443645-8443664 MS.gene028699:intron 50.0%
! GCATAAAAAGGCGTGGTGCA+AGG - chr2.1:8443731-8443750 MS.gene028699:intron 50.0%
! GGAACCAGGTTAGTCATCAC+TGG - chr2.1:8441629-8441648 MS.gene028699:CDS 50.0%
! TTCCTGGGCTTCTTGGCTTT+AGG + chr2.1:8443496-8443515 None:intergenic 50.0%
!! AGGGGCTTTTAAGAAGGTCC+TGG - chr2.1:8443777-8443796 MS.gene028699:intron 50.0%
!! GCCACGAGATTGTGCTGTTT+CGG - chr2.1:8443225-8443244 MS.gene028699:intron 50.0%
ACTGGATGACCCCGAGACAT+AGG - chr2.1:8443281-8443300 MS.gene028699:intron 55.0%
ATCTCGTGGCTGAAAGGGCA+AGG + chr2.1:8443215-8443234 None:intergenic 55.0%
CAGTGTTATCGCTGGGGCAT+TGG - chr2.1:8443652-8443671 MS.gene028699:intron 55.0%
CCTCAGCAGTGTTATCGCTG+GGG - chr2.1:8443646-8443665 MS.gene028699:intron 55.0%
CTAACCTGGTTCCCGAGAGA+AGG + chr2.1:8441622-8441641 None:intergenic 55.0%
CTCCTAAAGCCAAGAAGCCC+AGG - chr2.1:8443491-8443510 MS.gene028699:intron 55.0%
CTGGGAGGAAAATGAGCCGA+GGG - chr2.1:8443758-8443777 MS.gene028699:intron 55.0%
GAGAAGGGGAGAGAGAGACA+CGG + chr2.1:8442416-8442435 None:intergenic 55.0%
GCATCAGGCTGCGGTATAAC+CGG + chr2.1:8443376-8443395 None:intergenic 55.0%
GCTATCATCCTTCGGCTTCC+TGG + chr2.1:8443512-8443531 None:intergenic 55.0%
TCTCGTGGCTGAAAGGGCAA+GGG + chr2.1:8443214-8443233 None:intergenic 55.0%
TGGGAGGAAAATGAGCCGAG+GGG - chr2.1:8443759-8443778 MS.gene028699:intron 55.0%
!! GAGCCGAGGGGCTTTTAAGA+AGG - chr2.1:8443771-8443790 MS.gene028699:intron 55.0%
!! GGGCTTCTTGGCTTTAGGAG+TGG + chr2.1:8443491-8443510 None:intergenic 55.0%
!! TGTTATCGCTGGGGCATTGG+AGG - chr2.1:8443655-8443674 MS.gene028699:intron 55.0%
ACCGAGATTCCCCCCCTTGA+AGG + chr2.1:8443104-8443123 None:intergenic 60.0%
AGGCGTGGTGCAAGGATAGC+TGG - chr2.1:8443739-8443758 MS.gene028699:intron 60.0%
AGGCTGCGGTATAACCGGCA+AGG + chr2.1:8443371-8443390 None:intergenic 60.0%
CAAGAAGCCCAGGAAGCCGA+AGG - chr2.1:8443501-8443520 MS.gene028699:intron 60.0%
CCCCAGCGATAACACTGCTG+AGG + chr2.1:8443649-8443668 None:intergenic 60.0%
CCCTTCAAGGGGGGGAATCT+CGG - chr2.1:8443100-8443119 MS.gene028699:intron 60.0%
CCGAGATTCCCCCCCTTGAA+GGG + chr2.1:8443103-8443122 None:intergenic 60.0%
CTTCGGCTTCCTGGGCTTCT+TGG + chr2.1:8443503-8443522 None:intergenic 60.0%
GCTGGGAGGAAAATGAGCCG+AGG - chr2.1:8443757-8443776 MS.gene028699:intron 60.0%
GGCGTGGTGCAAGGATAGCT+GGG - chr2.1:8443740-8443759 MS.gene028699:intron 60.0%
GTGGTGCAAGGATAGCTGGG+AGG - chr2.1:8443743-8443762 MS.gene028699:intron 60.0%
!! ATCGCTGGGGCATTGGAGGC+TGG - chr2.1:8443659-8443678 MS.gene028699:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 8441597 8443897 8441597 ID=MS.gene028699
chr2.1 mRNA 8441597 8443897 8441597 ID=MS.gene028699.t1;Parent=MS.gene028699
chr2.1 exon 8443858 8443897 8443858 ID=MS.gene028699.t1.exon1;Parent=MS.gene028699.t1
chr2.1 CDS 8443858 8443897 8443858 ID=cds.MS.gene028699.t1;Parent=MS.gene028699.t1
chr2.1 exon 8441597 8442444 8441597 ID=MS.gene028699.t1.exon2;Parent=MS.gene028699.t1
chr2.1 CDS 8441597 8442444 8441597 ID=cds.MS.gene028699.t1;Parent=MS.gene028699.t1
Gene Sequence

>MS.gene028699

ATGGATTATTCATCAAGTAATTCCTTCTCTCGGGAACCAGGTTAGTCATCACTGGCTTATAAAATAGTATGTCCACATACTTCTTTTGAGGGTAACATTCTAAGTTTTACGTTTTTATATATGCATATGCTTGTTGTTAGGATTATATGTTATGCTTGGTTGATCAAGTGGAAAATAAAAATCATTTCTCATTAAGACACATGAATCACAAAAAAAATCGAGTCTCGTCTGGTGGATAATGAAACTCGAATGGATTCACTTATACTGCCTATTTTTTTCCACATAAGATTCAAATTAAGACCTTAGTGAGAAACCAAACTCTTTACTATTTGGATCAACATGTGTTGGCAATAAAAGTATATTTTCACATAACAAAGTCTACAAACATTAAAGATATCTGGATTGCGTTGTTCAACCTTCCAATTTTTTTTTTTCGCTCTTTATCTTCTCCACTCACTCAACCAAGCATAGATGTGGTGTTTGAACTGTAGAAGTTGATTCTAGTAGAGGTTTCAGGTTTCAATGAGATAATGAAATATCATAAAAAATTGAGATATGTATTCCTTTGACTTTGCTATATTTTTTAATTTTAAATAAAAAAATGAATTAAAATAAGGAGGCAAATATTTATATGTCTTAACATCGTTTCTCTCTCCCATTCATACATAACATAATTCTATCTCCTCTCTCATATCTATCTTTCTCTTTCATTCAGATTTCAATTTCAATCTTCTCTCTCCAGAAGGGCAAAACCTAAATACCGTTTGCACCCACCAAGTAAGTTTTCTCTCTCCTCTCTCCAATCTTTTTCTCTCTCCGTGTCTCTCTCTCCCCTTCTCTCACCCTCCTTTAAAATTTGAATACATGGGTCCTTTTTTCTCCTTTTGAACCTCCTTATAAATATTTTTTGTTCTGTAAATTTGTCTCTATTCACCATTTCAACCTAGATCTATTCTCATTTTTTGAAACCCTAAATTCAATTTTCGGATCTACGGCTGTTCAAAAAAAATAATTTCGGATCTACGTACCATGGACCAAATTCAAAATGTGGGAACTATAACAACATTCAGATCTAGGGGTTTCAACTTTGTGATGATTAAATTCATAAAGTTCCAACCTTTTTGCTTAAAAATGGTATTTTTAGATTCATTTCAGCTTGATCTATGAATTTGACTTTAATTATCATAACCCATTTTCGATTTTTATTTAATTTGTGCTTTGTTTTATGATTTTTATCCTTTTTCAATCTGCTTGTAGGGTTTATGTGGTTAATTGATCTATAATTTACTTTGTTATATTGATACTTTATGTATTCTGTTATGTTTATTTATTTGGTTTTGTTCAAATTGATGAACTCTATTGCTTCTAGAGTAATTTGCTGCTTAGTAAGGAAAAAAAAGTTTTTTTTTTTGTTAAATGAAATTTTATAGGATCGTTTGTTGTTGTTTTGTAGTTGTCATGGATGATGATGTGCTGAATATGCCCAATTGGGGTTACTATGAACCCTTCAAGGGGGGGAATCTCGGTCTGAACCTCATGCCTGCCATGAATGATCGTGAAACAAAACCATTTTTTCCTGGTCGTGATCCAGCTATGATAGGAAGTGCAAATGGGCCCTTGCCCTTTCAGCCACGAGATTGTGCTGTTTCGGAGTCTGCTACTCCGTTTAACTATGTGAGGGATAACTGGATGACCCCGAGACATAGGTTTATCAATATGCAGCTGACAAATCCAAATTATGTTGTTCTTCCAGAAGCATCACAATCACTCTCCTTGCCGGTTATACCGCAGCCTGATGCATCAAGAAATGAGAATGCGGAGAATGCTGAAGAATTGAGTGTTAAGAAGGAAGGTGGCAAAGCGAAGAAAAGGAAGTCTAAGGGTGCTGTTACCACTCCTAAAGCCAAGAAGCCCAGGAAGCCGAAGGATGATAGCAGTGTTTCAGCTCAAGTTGTGAAACCACCGAAGAAGACTTTGGCACTTGAAATCAATGGAATTGAAATGGACATTTCTGGTCTACCAGTTCCAGTTTGTTCTTGCACTGGGAATCCTCAGCAGTGTTATCGCTGGGGCATTGGAGGCTGGCAATCAGCTTGCTGTACCACAAATGTGTCAATTTATCCTTTGCCGATGAGCATAAAAAGGCGTGGTGCAAGGATAGCTGGGAGGAAAATGAGCCGAGGGGCTTTTAAGAAGGTCCTGGAAAAACTTGCTGCTGAAGGTTATAATTTTGCTAATGCTATTGATCTGAAGACTCATTGGGCTAGACATGGCACCAACAAGTTTGTCACTATCAGATAG
Protein sequence

>MS.gene028699.t1

MDYSSSNSFSREPVVMDDDVLNMPNWGYYEPFKGGNLGLNLMPAMNDRETKPFFPGRDPAMIGSANGPLPFQPRDCAVSESATPFNYVRDNWMTPRHRFINMQLTNPNYVVLPEASQSLSLPVIPQPDASRNENAENAEELSVKKEGGKAKKRKSKGAVTTPKAKKPRKPKDDSSVSAQVVKPPKKTLALEINGIEMDISGLPVPVCSCTGNPQQCYRWGIGGWQSACCTTNVSIYPLPMSIKRRGARIAGRKMSRGAFKKVLEKLAAEGYNFANAIDLKTHWARHGTNKFVTIR