Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028891.t1 | XP_013465109.1 | 82 | 217 | 32 | 2 | 1 | 210 | 1 | 217 | 7.50E-87 | 330.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028891.t1 | F4JIJ7 | 40.6 | 180 | 87 | 5 | 12 | 174 | 11 | 187 | 1.8e-25 | 117.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028891.t1 | A0A072VAT4 | 82.0 | 217 | 32 | 2 | 1 | 210 | 1 | 217 | 5.4e-87 | 330.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene028891.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028891.t1 | MTR_2g090440 | 85.253 | 217 | 25 | 3 | 1 | 210 | 1 | 217 | 6.61e-128 | 359 |
| MS.gene028891.t1 | MTR_2g090480 | 81.279 | 219 | 32 | 2 | 1 | 210 | 1 | 219 | 2.47e-123 | 348 |
| MS.gene028891.t1 | MTR_2g090425 | 80.693 | 202 | 29 | 2 | 1 | 192 | 1 | 202 | 2.24e-109 | 317 |
| MS.gene028891.t1 | MTR_2g090500 | 86.550 | 171 | 17 | 2 | 46 | 210 | 60 | 230 | 6.76e-99 | 286 |
| MS.gene028891.t1 | MTR_4g078350 | 65.979 | 194 | 58 | 4 | 9 | 194 | 8 | 201 | 2.45e-82 | 244 |
| MS.gene028891.t1 | MTR_2g013920 | 45.143 | 175 | 83 | 4 | 7 | 173 | 2 | 171 | 2.34e-44 | 146 |
| MS.gene028891.t1 | MTR_2g090515 | 78.182 | 55 | 12 | 0 | 1 | 55 | 1 | 55 | 3.14e-23 | 89.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028891.t1 | AT4G20970 | 40.884 | 181 | 87 | 6 | 12 | 175 | 11 | 188 | 6.93e-34 | 120 |
| MS.gene028891.t1 | AT1G10585 | 28.902 | 173 | 117 | 3 | 7 | 177 | 9 | 177 | 1.44e-14 | 69.3 |
| MS.gene028891.t1 | AT1G10586 | 25.882 | 170 | 120 | 3 | 7 | 174 | 9 | 174 | 2.40e-11 | 60.5 |
Find 40 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACAAACTTTGTTACAATT+TGG | 0.173105 | 2.3:+9922827 | MS.gene028891:CDS |
| TATCTCTTCAAATTCTTCTT+TGG | 0.234992 | 2.3:+9922087 | MS.gene028891:CDS |
| CAATTGTGGGATCTTGAAAT+TGG | 0.247122 | 2.3:+9922920 | MS.gene028891:CDS |
| ATGCTTCTTAATTAAGGATT+TGG | 0.262973 | 2.3:-9922989 | None:intergenic |
| AACAAGAAAGAAAGTTTAAA+AGG | 0.273993 | 2.3:+9921932 | MS.gene028891:CDS |
| AGAGAAGAAAGAAAGTTTAA+TGG | 0.275748 | 2.3:+9921907 | MS.gene028891:CDS |
| GAGAGTTAAGTTTGGAATAG+AGG | 0.305206 | 2.3:-9921173 | None:intergenic |
| GAGAAGAAAGAAAGTTTAAT+GGG | 0.355968 | 2.3:+9921908 | MS.gene028891:CDS |
| CCAACTTTAGATTTGTCTCT+AGG | 0.356186 | 2.3:-9921880 | None:intergenic |
| AGGAAGAAGAGAGTTAAGTT+TGG | 0.360183 | 2.3:-9921181 | None:intergenic |
| CCAAATTGTAACAAAGTTTG+TGG | 0.389197 | 2.3:-9922827 | None:intergenic |
| CTACAAGTGATTGTAACATA+TGG | 0.392190 | 2.3:+9922001 | MS.gene028891:CDS |
| AACATCCTTTCTTTCGACTT+TGG | 0.397896 | 2.3:-9921121 | None:intergenic |
| TCTTCAAATTCTTCTTTGGC+TGG | 0.403407 | 2.3:+9922091 | MS.gene028891:CDS |
| TTGAAATTGGAAGTGAGAGT+TGG | 0.404544 | 2.3:+9922933 | MS.gene028891:CDS |
| TAATTTCACAGAAAATAAAA+TGG | 0.425918 | 2.3:-9922033 | None:intergenic |
| TACAAGTGATTGTAACATAT+GGG | 0.426502 | 2.3:+9922002 | MS.gene028891:CDS |
| ATAGCCTCATCTACTTGATC+AGG | 0.429867 | 2.3:-9921842 | None:intergenic |
| CTTTCGACTTTGGTTGAACA+AGG | 0.449950 | 2.3:-9921111 | None:intergenic |
| TGAAATTGGAAGTGAGAGTT+GGG | 0.469657 | 2.3:+9922934 | MS.gene028891:CDS |
| TCTTCATACCGTGCATGCTC+AGG | 0.472764 | 2.3:+9922123 | MS.gene028891:CDS |
| AAAATAGAGATTCATGAAAT+GGG | 0.475198 | 2.3:+9921971 | MS.gene028891:CDS |
| TCTTGTTGATTATGTTCCAT+TGG | 0.483862 | 2.3:-9921081 | None:intergenic |
| TTCAACCAAAGTCGAAAGAA+AGG | 0.492351 | 2.3:+9921116 | MS.gene028891:CDS |
| CCTAGAGACAAATCTAAAGT+TGG | 0.498284 | 2.3:+9921880 | MS.gene028891:CDS |
| ATTTCAAGATCCCACAATTG+AGG | 0.509351 | 2.3:-9922917 | None:intergenic |
| TTAAGGATTTGGTAAACTCA+TGG | 0.514490 | 2.3:-9922978 | None:intergenic |
| TCATCTACTTGATCAGGTAA+TGG | 0.522136 | 2.3:-9921836 | None:intergenic |
| GGAAACACAGCCTCAATTGT+GGG | 0.559018 | 2.3:+9922907 | MS.gene028891:CDS |
| TGGAAACACAGCCTCAATTG+TGG | 0.568137 | 2.3:+9922906 | MS.gene028891:CDS |
| ATGACCTTCGAATTATAGCT+AGG | 0.581881 | 2.3:-9921201 | None:intergenic |
| TCTTCCTAGCTATAATTCGA+AGG | 0.587922 | 2.3:+9921197 | MS.gene028891:CDS |
| TATATATACCTGAGCATGCA+CGG | 0.590008 | 2.3:-9922131 | None:intergenic |
| GAGCAATCAAAATAAGTGAG+AGG | 0.593941 | 2.3:+9922849 | MS.gene028891:CDS |
| AGGTTGAAAGAGTTTGTGAA+CGG | 0.618184 | 2.3:+9922869 | MS.gene028891:CDS |
| TGTTACAATCACTTGTAGAG+TGG | 0.619858 | 2.3:-9921996 | None:intergenic |
| ATTACCTGATCAAGTAGATG+AGG | 0.636526 | 2.3:+9921838 | MS.gene028891:CDS |
| ACAAGTGATTGTAACATATG+GGG | 0.655911 | 2.3:+9922003 | MS.gene028891:CDS |
| GAACGGATCTGTGAGCGATG+TGG | 0.701045 | 2.3:+9922886 | MS.gene028891:CDS |
| ACTGCATGAAGAGAACGTCG+AGG | 0.768331 | 2.3:+9922063 | MS.gene028891:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAAAATGTATAAATAAAT+AGG | + | chr2.3:9921609-9921628 | MS.gene028891:intron | 10.0% |
| !! | TAATTAGAGTTATATTGTAT+TGG | + | chr2.3:9921672-9921691 | MS.gene028891:intron | 15.0% |
| !! | TAATTTCACAGAAAATAAAA+TGG | - | chr2.3:9922036-9922055 | None:intergenic | 15.0% |
| !! | TTATTAAATAATGCTACATT+AGG | - | chr2.3:9921533-9921552 | None:intergenic | 15.0% |
| !!! | AAAATTTTTTCTTTGACTTT+TGG | - | chr2.3:9922439-9922458 | None:intergenic | 15.0% |
| !!! | AATTTAGAGCTTATTTTTTA+GGG | - | chr2.3:9922514-9922533 | None:intergenic | 15.0% |
| !!! | ATTTATTTTTCTAATTTTGG+CGG | - | chr2.3:9921378-9921397 | None:intergenic | 15.0% |
| !!! | CAGCTTTAATAATAATATAA+GGG | - | chr2.3:9922214-9922233 | None:intergenic | 15.0% |
| !!! | TGTTATTTTTAATTTAATGC+AGG | + | chr2.3:9921802-9921821 | MS.gene028891:intron | 15.0% |
| !!! | TTCTGATTTGATTATTAATT+TGG | + | chr2.3:9922795-9922814 | MS.gene028891:intron | 15.0% |
| !!! | TTTTTTTTCGTAATAAGTTT+TGG | - | chr2.3:9922560-9922579 | None:intergenic | 15.0% |
| !! | AAAAATAGAGATTCATGAAA+TGG | + | chr2.3:9921970-9921989 | MS.gene028891:CDS | 20.0% |
| !! | AAAATAGAGATTCATGAAAT+GGG | + | chr2.3:9921971-9921990 | MS.gene028891:CDS | 20.0% |
| !! | AAAGTGGAATATATTGTATT+GGG | + | chr2.3:9921632-9921651 | MS.gene028891:intron | 20.0% |
| !! | AACAAGAAAGAAAGTTTAAA+AGG | + | chr2.3:9921932-9921951 | MS.gene028891:CDS | 20.0% |
| !! | CGAAAAGTTTGAATATTTAT+AGG | + | chr2.3:9922470-9922489 | MS.gene028891:intron | 20.0% |
| !! | TAAAAAGATAAGGTTGAATA+TGG | - | chr2.3:9922291-9922310 | None:intergenic | 20.0% |
| !! | TGTATAAATAAATAGGAAAG+TGG | + | chr2.3:9921616-9921635 | MS.gene028891:intron | 20.0% |
| !! | TTTAACGCTATAAAAAGATA+AGG | - | chr2.3:9922301-9922320 | None:intergenic | 20.0% |
| !!! | ATTTCATGAATCTCTATTTT+TGG | - | chr2.3:9921971-9921990 | None:intergenic | 20.0% |
| !!! | CAATTTAGAGCTTATTTTTT+AGG | - | chr2.3:9922515-9922534 | None:intergenic | 20.0% |
| !!! | GACACTTTTTTTTAGAAAAT+GGG | + | chr2.3:9921742-9921761 | MS.gene028891:intron | 20.0% |
| !!! | GCAGCTTTAATAATAATATA+AGG | - | chr2.3:9922215-9922234 | None:intergenic | 20.0% |
| !!! | TAACATAGATTATTTTGTGA+AGG | + | chr2.3:9921433-9921452 | MS.gene028891:intron | 20.0% |
| !!! | TGAAATGACTCAATTATTTT+GGG | + | chr2.3:9921720-9921739 | MS.gene028891:intron | 20.0% |
| !!! | TTATTATTCAAATGCACTTT+TGG | - | chr2.3:9922662-9922681 | None:intergenic | 20.0% |
| !!! | TTGAAATGACTCAATTATTT+TGG | + | chr2.3:9921719-9921738 | MS.gene028891:intron | 20.0% |
| !!! | TTTTATAGCGTTAAAAGATA+GGG | + | chr2.3:9922306-9922325 | MS.gene028891:intron | 20.0% |
| !!! | TTTTTATAGCGTTAAAAGAT+AGG | + | chr2.3:9922305-9922324 | MS.gene028891:intron | 20.0% |
| ! | ACTAGACTTGAATACATATT+TGG | + | chr2.3:9922262-9922281 | MS.gene028891:intron | 25.0% |
| ! | AGAGAAGAAAGAAAGTTTAA+TGG | + | chr2.3:9921907-9921926 | MS.gene028891:CDS | 25.0% |
| ! | GAAAGTGGAATATATTGTAT+TGG | + | chr2.3:9921631-9921650 | MS.gene028891:intron | 25.0% |
| ! | GAGAAGAAAGAAAGTTTAAT+GGG | + | chr2.3:9921908-9921927 | MS.gene028891:CDS | 25.0% |
| ! | TACAAGTGATTGTAACATAT+GGG | + | chr2.3:9922002-9922021 | MS.gene028891:CDS | 25.0% |
| ! | TATCTCTTCAAATTCTTCTT+TGG | + | chr2.3:9922087-9922106 | MS.gene028891:CDS | 25.0% |
| !! | CTCTTAACAATATTTTGCAA+AGG | + | chr2.3:9922710-9922729 | MS.gene028891:intron | 25.0% |
| !!! | GGACACTTTTTTTTAGAAAA+TGG | + | chr2.3:9921741-9921760 | MS.gene028891:intron | 25.0% |
| !!! | GTGATGATGTAAACTTTTAA+GGG | + | chr2.3:9922412-9922431 | MS.gene028891:intron | 25.0% |
| !!! | TAAAAATTTTTTCGCACGTT+TGG | - | chr2.3:9922637-9922656 | None:intergenic | 25.0% |
| !!! | TCAAGTCTAGTTTTAAACTA+CGG | - | chr2.3:9922253-9922272 | None:intergenic | 25.0% |
| !!! | TGTGATGATGTAAACTTTTA+AGG | + | chr2.3:9922411-9922430 | MS.gene028891:intron | 25.0% |
| !!! | TGTTAGATAGTTTAGTCTTA+TGG | - | chr2.3:9921418-9921437 | None:intergenic | 25.0% |
| ACAAGTGATTGTAACATATG+GGG | + | chr2.3:9922003-9922022 | MS.gene028891:CDS | 30.0% | |
| ACGGATGAAGTATATTACTT+TGG | + | chr2.3:9921779-9921798 | MS.gene028891:intron | 30.0% | |
| AGAAAATGGGTCAAATATTC+CGG | + | chr2.3:9921755-9921774 | MS.gene028891:intron | 30.0% | |
| ATTAAAGCTGCAATTGCAAT+TGG | + | chr2.3:9922224-9922243 | MS.gene028891:intron | 30.0% | |
| CAAACTGAGTCAGATAAAAT+GGG | - | chr2.3:9921572-9921591 | None:intergenic | 30.0% | |
| CCAAATTGTAACAAAGTTTG+TGG | - | chr2.3:9922830-9922849 | None:intergenic | 30.0% | |
| CCACAAACTTTGTTACAATT+TGG | + | chr2.3:9922827-9922846 | MS.gene028891:CDS | 30.0% | |
| CTACAAGTGATTGTAACATA+TGG | + | chr2.3:9922001-9922020 | MS.gene028891:CDS | 30.0% | |
| GAAAGACTAAATCATGTTTC+TGG | + | chr2.3:9922599-9922618 | MS.gene028891:intron | 30.0% | |
| GAAAGGATGTTGAGAAAAAT+AGG | + | chr2.3:9921133-9921152 | MS.gene028891:CDS | 30.0% | |
| GATGTAAATAATGCAACATG+TGG | + | chr2.3:9921310-9921329 | MS.gene028891:intron | 30.0% | |
| ! | TTAAGGATTTGGTAAACTCA+TGG | - | chr2.3:9922981-9923000 | None:intergenic | 30.0% |
| !! | GTTTAGTCTTATGGTATGTT+AGG | - | chr2.3:9921409-9921428 | None:intergenic | 30.0% |
| !! | TAAGCTCTAAATTGAGTTGT+AGG | + | chr2.3:9922521-9922540 | MS.gene028891:intron | 30.0% |
| AACATCCTTTCTTTCGACTT+TGG | - | chr2.3:9921124-9921143 | None:intergenic | 35.0% | |
| AGGAAGAAGAGAGTTAAGTT+TGG | - | chr2.3:9921184-9921203 | None:intergenic | 35.0% | |
| AGGTTGAAAGAGTTTGTGAA+CGG | + | chr2.3:9922869-9922888 | MS.gene028891:CDS | 35.0% | |
| ATGACCTTCGAATTATAGCT+AGG | - | chr2.3:9921204-9921223 | None:intergenic | 35.0% | |
| ATTACCTGATCAAGTAGATG+AGG | + | chr2.3:9921838-9921857 | MS.gene028891:CDS | 35.0% | |
| ATTTCAAGATCCCACAATTG+AGG | - | chr2.3:9922920-9922939 | None:intergenic | 35.0% | |
| CAATTGTGGGATCTTGAAAT+TGG | + | chr2.3:9922920-9922939 | MS.gene028891:CDS | 35.0% | |
| CCAAACTGAGTCAGATAAAA+TGG | - | chr2.3:9921573-9921592 | None:intergenic | 35.0% | |
| CCAACTTTAGATTTGTCTCT+AGG | - | chr2.3:9921883-9921902 | None:intergenic | 35.0% | |
| GAAAATGGGTCAAATATTCC+GGG | + | chr2.3:9921756-9921775 | MS.gene028891:intron | 35.0% | |
| GAGCAATCAAAATAAGTGAG+AGG | + | chr2.3:9922849-9922868 | MS.gene028891:CDS | 35.0% | |
| TATATATACCTGAGCATGCA+CGG | - | chr2.3:9922134-9922153 | None:intergenic | 35.0% | |
| TCATCTACTTGATCAGGTAA+TGG | - | chr2.3:9921839-9921858 | None:intergenic | 35.0% | |
| TCTTCAAATTCTTCTTTGGC+TGG | + | chr2.3:9922091-9922110 | MS.gene028891:CDS | 35.0% | |
| TCTTCCTAGCTATAATTCGA+AGG | + | chr2.3:9921197-9921216 | MS.gene028891:CDS | 35.0% | |
| TGAAATTGGAAGTGAGAGTT+GGG | + | chr2.3:9922934-9922953 | MS.gene028891:CDS | 35.0% | |
| TGTTACAATCACTTGTAGAG+TGG | - | chr2.3:9921999-9922018 | None:intergenic | 35.0% | |
| TTCAACCAAAGTCGAAAGAA+AGG | + | chr2.3:9921116-9921135 | MS.gene028891:CDS | 35.0% | |
| TTGAAATTGGAAGTGAGAGT+TGG | + | chr2.3:9922933-9922952 | MS.gene028891:CDS | 35.0% | |
| ! | CCATTTTATCTGACTCAGTT+TGG | + | chr2.3:9921570-9921589 | MS.gene028891:intron | 35.0% |
| ! | CCTAGAGACAAATCTAAAGT+TGG | + | chr2.3:9921880-9921899 | MS.gene028891:CDS | 35.0% |
| ! | GAGAGTTAAGTTTGGAATAG+AGG | - | chr2.3:9921176-9921195 | None:intergenic | 35.0% |
| ATAGCCTCATCTACTTGATC+AGG | - | chr2.3:9921845-9921864 | None:intergenic | 40.0% | |
| CTGAGTCAGATAAAATGGGA+CGG | - | chr2.3:9921568-9921587 | None:intergenic | 40.0% | |
| CTTTCGACTTTGGTTGAACA+AGG | - | chr2.3:9921114-9921133 | None:intergenic | 40.0% | |
| GTAATATACTTCATCCGTCC+CGG | - | chr2.3:9921777-9921796 | None:intergenic | 40.0% | |
| AGTCAGATAAAATGGGACGG+AGG | - | chr2.3:9921565-9921584 | None:intergenic | 45.0% | |
| ATGGGTCAAATATTCCGGGA+CGG | + | chr2.3:9921760-9921779 | MS.gene028891:intron | 45.0% | |
| GGAAACACAGCCTCAATTGT+GGG | + | chr2.3:9922907-9922926 | MS.gene028891:CDS | 45.0% | |
| GTCAGATAAAATGGGACGGA+GGG | - | chr2.3:9921564-9921583 | None:intergenic | 45.0% | |
| TGGAAACACAGCCTCAATTG+TGG | + | chr2.3:9922906-9922925 | MS.gene028891:CDS | 45.0% | |
| !!! | TAAATTTATTTTTCTAATTT+TGG | - | chr2.3:9921381-9921400 | None:intergenic | 5.0% |
| ACTGCATGAAGAGAACGTCG+AGG | + | chr2.3:9922063-9922082 | MS.gene028891:CDS | 50.0% | |
| TCTTCATACCGTGCATGCTC+AGG | + | chr2.3:9922123-9922142 | MS.gene028891:CDS | 50.0% | |
| GAACGGATCTGTGAGCGATG+TGG | + | chr2.3:9922886-9922905 | MS.gene028891:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 9921084 | 9923000 | 9921084 | ID=MS.gene028891 |
| chr2.3 | mRNA | 9921084 | 9923000 | 9921084 | ID=MS.gene028891.t1;Parent=MS.gene028891 |
| chr2.3 | exon | 9921084 | 9921218 | 9921084 | ID=MS.gene028891.t1.exon1;Parent=MS.gene028891.t1 |
| chr2.3 | CDS | 9921084 | 9921218 | 9921084 | ID=cds.MS.gene028891.t1;Parent=MS.gene028891.t1 |
| chr2.3 | exon | 9921824 | 9922144 | 9921824 | ID=MS.gene028891.t1.exon2;Parent=MS.gene028891.t1 |
| chr2.3 | CDS | 9921824 | 9922144 | 9921824 | ID=cds.MS.gene028891.t1;Parent=MS.gene028891.t1 |
| chr2.3 | exon | 9922824 | 9923000 | 9922824 | ID=MS.gene028891.t1.exon3;Parent=MS.gene028891.t1 |
| chr2.3 | CDS | 9922824 | 9923000 | 9922824 | ID=cds.MS.gene028891.t1;Parent=MS.gene028891.t1 |
| Gene Sequence |
| Protein sequence |
